Rh4BG126300

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000654
Physical Location & Seq
Forward (+)
21114399 .. 21115070
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG126300.1

Sequence Viewer

Length: 672 bp
ATGAAACAGGTGTTGTCTGAATGTGGCATATCTAGCATTTACCTAAACGGAAACGAGATTCCAGATTGGTTTCGCCACAGGAGAATGGGCAACTCTGTGTTGTCTATTATAGTACCTCCACATCTTAATCATAGAATTCGAGGCTTAAATGCATGTGTTGTATATGCACGACGGCCAGATATTGGTCGTTCTGCTAAATATCAAAACTTGTTTAAAGTCAGCAATGAGACCAAGGGTCTCATGTGGACCTATTCCCCTATCACAATAGGTATTCCAAAGGAGAATGAAGACATGTTATGGCTAAGCCATTGGAGGTTTGGAGAACATGATCTGGGAGGGGGAGACGAAGTACGTGTTTCGGTTGAGTTTGGATCCGAGCTTGGATACAATCTAAATTTATGGACAAAGGAATTGGGAATCCAACTTGTGTATGAGCAGGAAAGTAGTGAAGATGGAAAGAAACACAATTTCCTTGCTGGAGATGTATCAGTGTCATCCTCAAGTTACCAAATGTGGACGGGAAAATACTTTCTTTGTAATAACCTGAATCGTGCTCAGAAAACACAATTCAAGAGTAGCCAGGAAAACCCATCTCATCTTGATTCTGACAAAACCAAGAGCCCCTTCCTTTTCTTATTTGAGCATGATGATGTTTCACAGGCTGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

223

Amino Acids

25.56

Weight (kDa)

6.3

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 16 - 148 9.8e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 182
AclWI GGATC 2 cut(s) 366, 379
AcoI YGGCCR 1 cut(s) 173
AcsI RAATTY 2 cut(s) 135, 394
AfaI GTAC 2 cut(s) 114, 351
AfiI CCNNNNNNNGG 1 cut(s) 182
AflIII ACRYGT 2 cut(s) 291, 352
AgsI TTSAA 1 cut(s) 571
AhdI GACNNNNNGTC 1 cut(s) 234
AjnI CCWGG 1 cut(s) 579
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 1 cut(s) 379
AluI AGCT 1 cut(s) 379
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 3 cut(s) 221, 242, 336
AlwI GGATC 2 cut(s) 366, 379
AoxI GGCC 1 cut(s) 173
ApoI RAATTY 2 cut(s) 135, 394
AspS9I GGNCC 1 cut(s) 246
AvaII GGWCC 1 cut(s) 246
BamHI GGATCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 623
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 556
BccI CCATC 2 cut(s) 446, 598
BceAI ACGGC 1 cut(s) 188
BciT130I CCWGG 1 cut(s) 581
BciVI GTATCC 1 cut(s) 377
BclI TGATCA 1 cut(s) 664
BcoDI GTCTC 3 cut(s) 221, 242, 336
BfaI CTAG 1 cut(s) 33
BfuI GTATCC 1 cut(s) 377
BlpI GCTNAGC 1 cut(s) 302
Bme1390I CCNGG 1 cut(s) 581
Bme18I GGWCC 1 cut(s) 246
BmeRI GACNNNNNGTC 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 1 cut(s) 581
BpiI GAAGAC 1 cut(s) 294
BpmI CTGGAG 1 cut(s) 498
Bpu1102I GCTNAGC 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 484
BsaAI YACGTR 1 cut(s) 353
BsaI GGTCTC 2 cut(s) 221, 242
BsaJI CCNNGG 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse3DI GCAATG 1 cut(s) 229
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 1 cut(s) 494
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 569
BshFI GGCC 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 556
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 3 cut(s) 221, 242, 336
BsmBI CGTCTC 1 cut(s) 336
BsnI GGCC 1 cut(s) 175
Bso31I GGTCTC 2 cut(s) 221, 242
Bsp1286I GDGCHC 2 cut(s) 556, 623
Bsp143I GATC 3 cut(s) 328, 371, 664
Bsp1720I GCTNAGC 1 cut(s) 302
BspANI GGCC 1 cut(s) 175
BspCNI CTCAG 1 cut(s) 568
BspLI GGNNCC 1 cut(s) 373
BspPI GGATC 2 cut(s) 366, 379
BspTNI GGTCTC 2 cut(s) 221, 242
BsrDI GCAATG 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 3 cut(s) 328, 371, 664
BssT1I CCWWGG 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 581
BstBAI YACGTR 1 cut(s) 353
BstDEI CTNAG 2 cut(s) 302, 555
BstF5I GGATG 1 cut(s) 494
BstKTI GATC 3 cut(s) 331, 374, 667
BstMAI GTCTC 3 cut(s) 221, 242, 336
BstMBI GATC 3 cut(s) 328, 371, 664
BstMWI GCNNNNNNNGC 1 cut(s) 33
BstNI CCWGG 1 cut(s) 581
BstNSI RCATGY 2 cut(s) 156, 295
BstSCI CCNGG 1 cut(s) 579
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 1 cut(s) 371
BstYI RGATCY 1 cut(s) 371
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 1 cut(s) 175
BtsCI GGATG 1 cut(s) 494
BtsIMutI CAGTG 1 cut(s) 495
Cfr13I GGNCC 1 cut(s) 246
Csp6I GTAC 2 cut(s) 113, 350
CviAII CATG 5 cut(s) 153, 241, 292, 326, 644
CviJI RGCY 8 cut(s) 144, 175, 301, 306, 379, 579, 621, 662
CviKI_1 RGCY 8 cut(s) 144, 175, 301, 306, 379, 579, 621, 662
CviQI GTAC 2 cut(s) 113, 350
DdeI CTNAG 2 cut(s) 302, 555
DpnI GATC 3 cut(s) 330, 373, 666
DpnII GATC 3 cut(s) 328, 371, 664
DraI TTTAAA 1 cut(s) 214
DriI GACNNNNNGTC 1 cut(s) 234
EaeI YGGCCR 1 cut(s) 173
Eam1105I GACNNNNNGTC 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 623
Eco31I GGTCTC 2 cut(s) 221, 242
Eco47I GGWCC 1 cut(s) 246
EcoRI GAATTC 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 579
EcoT14I CCWWGG 1 cut(s) 231
EcoT22I ATGCAT 1 cut(s) 154
EcoT38I GRGCYC 1 cut(s) 623
ErhI CCWWGG 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 5 cut(s) 156, 244, 295, 329, 647
FalI AAGNNNNNCTT 2 cut(s) 608, 640
FatI CATG 5 cut(s) 152, 240, 291, 325, 643
FbaI TGATCA 1 cut(s) 664
FokI GGATG 1 cut(s) 481
FriOI GRGCYC 1 cut(s) 623
FspBI CTAG 1 cut(s) 33
GsuI CTGGAG 1 cut(s) 498
HaeIII GGCC 1 cut(s) 175
Hin1II CATG 5 cut(s) 156, 244, 295, 329, 647
HinfI GANTC 4 cut(s) 58, 417, 547, 602
Hpy166II GTNNAC 2 cut(s) 246, 516
Hpy188I TCNGA 4 cut(s) 19, 376, 558, 607
Hpy188III TCNNGA 3 cut(s) 62, 571, 599
Hpy8I GTNNAC 2 cut(s) 246, 516
Hpy99I CGWCG 1 cut(s) 174
HpyAV CCTTC 1 cut(s) 634
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 2 cut(s) 152, 167
HpyF10VI GCNNNNNNNGC 1 cut(s) 33
HpyF3I CTNAG 2 cut(s) 302, 555
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 5 cut(s) 156, 244, 295, 329, 647
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 3 cut(s) 328, 371, 664
MaeI CTAG 1 cut(s) 33
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 1 cut(s) 503
MalI GATC 3 cut(s) 330, 373, 666
MboI GATC 3 cut(s) 328, 371, 664
MboII GAAGA 2 cut(s) 299, 461
MflI RGATCY 1 cut(s) 371
MhlI GDGCHC 2 cut(s) 556, 623
MluCI AATT 5 cut(s) 135, 394, 410, 466, 566
MmeI TCCRAC 1 cut(s) 445
MnlI CCTC 5 cut(s) 126, 134, 306, 329, 508
Mph1103I ATGCAT 1 cut(s) 154
MseI TTAA 3 cut(s) 126, 146, 213
MslI CAYNNNNRTG 1 cut(s) 648
MspR9I CCNGG 1 cut(s) 581
MvaI CCWGG 1 cut(s) 581
MwoI GCNNNNNNNGC 1 cut(s) 33
NdeII GATC 3 cut(s) 328, 371, 664
NlaIII CATG 5 cut(s) 156, 244, 295, 329, 647
NlaIV GGNNCC 1 cut(s) 373
NsiI ATGCAT 1 cut(s) 154
NspI RCATGY 2 cut(s) 156, 295
PciI ACATGT 1 cut(s) 291
PfeI GAWTC 4 cut(s) 58, 417, 547, 602
PflMI CCANNNNNTGG 1 cut(s) 182
Ppu21I YACGTR 1 cut(s) 353
PscI ACATGT 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 1 cut(s) 373
PspPI GGNCC 1 cut(s) 246
PsuI RGATCY 1 cut(s) 371
RsaI GTAC 2 cut(s) 114, 351
RsaNI GTAC 2 cut(s) 113, 350
RseI CAYNNNNRTG 1 cut(s) 648
SaqAI TTAA 3 cut(s) 126, 146, 213
Sau3AI GATC 3 cut(s) 328, 371, 664
Sau96I GGNCC 1 cut(s) 246
ScrFI CCNGG 1 cut(s) 581
SduI GDGCHC 2 cut(s) 556, 623
SetI ASST 9 cut(s) 12, 45, 118, 251, 271, 317, 355, 381, 546
SinI GGWCC 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 648
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
Sse9I AATT 5 cut(s) 135, 394, 410, 466, 566
SspMI CTAG 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 579
StyI CCWWGG 1 cut(s) 231
TaiI ACGT 1 cut(s) 355
TaqI TCGA 1 cut(s) 139
TasI AATT 5 cut(s) 135, 394, 410, 466, 566
TfiI GAWTC 4 cut(s) 58, 417, 547, 602
Tru1I TTAA 3 cut(s) 126, 146, 213
Tru9I TTAA 3 cut(s) 126, 146, 213
TscAI CASTG 1 cut(s) 495
TspDTI ATGAA 2 cut(s) 17, 300
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 1 cut(s) 495
Van91I CCANNNNNTGG 1 cut(s) 182
VpaK11BI GGWCC 1 cut(s) 246
XapI RAATTY 2 cut(s) 135, 394
XceI RCATGY 2 cut(s) 156, 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
XspI CTAG 1 cut(s) 33
Zsp2I ATGCAT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.