Rh4BG126200

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000654
Physical Location & Seq
Forward (+)
21113572 .. 21114363
792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG126200.1

Sequence Viewer

Length: 792 bp
ATGAAGCTTCCGACCAGAATCAAAATGTCAAGTTCTCTTCAAACACTTATTCTTTCTGGCTGCTCAAAGCTTGTGCGTCATTCTAATATGGTTGCAACAAATCAGTCACTCTTGACAGCTAGTGATATGAAGAAAGACGATTGTTTATCACCTAACTTGTGGAAATCGTTCTTATCATGGGCAGTACCAAGCAGAAATATCATACCAACCAGTTTCTCAGTATCAAATTTACCACGTTCTTTACGAAGCTTAAGCTTAGCTGACTGCAACCTATTACAGATTCCAGGTGATCTTAGTATCCTCTCCTCATTGAAGTATTTAGACCTATGTGGGAACCCAATTCAGAGCCTCCCAGAAAACATAAGATCTCTTAGTAAGCTTGAGACACTTATGTTAGAGGACTGCACAGAACTCAGAACGCTTCCAGAGCTCCCACCAAGTCTCCAGAGTTTGCTTGCAAGTTCTTGTAAATCATTGAAAAGAATAACAAATTTACCAAACTTGTTCAAATCATTGGATACAGATTTTCTGGATTGCAAGAAGTTAGTTGAAGTTGAAAGCTTGTTCACTATAAAACCATTGACAACCACTGACATAGAAATGATCAAAGATATGGGACTGTTCAATTTTAAATCCATTGGAAGCTATGCTGAGGTTGAAATGATCAACTACATTACAAATACAACCAAGAAGGTCCCCATCCAGGTTCTCTCCTTCCTCTATCACTCATGTATCCTTGTTTTAATTATAATTTGTATGATACAAATGCTAGCTAGCTGTTTGAATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

263

Amino Acids

29.36

Weight (kDa)

8.49

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 79 - 172 6.6e-10 Leucine-rich repeat region
LRR_8 PF13855 79 - 135 4e-07 Leucine rich repeat
LRR_4 PF12799 103 - 145 2.4e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 749
AcsI RAATTY 2 cut(s) 226, 490
AfaI GTAC 1 cut(s) 186
AfiI CCNNNNNNNGG 1 cut(s) 703
AflII CTTAAG 1 cut(s) 250
AgsI TTSAA 9 cut(s) 41, 313, 478, 508, 551, 557, 625, 659, 784
AjnI CCWGG 2 cut(s) 283, 702
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 2 cut(s) 377, 446
ApeKI GCWGC 1 cut(s) 60
ApoI RAATTY 2 cut(s) 226, 490
Asp700I GAANNNNTTC 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 694
AsuHPI GGTGA 2 cut(s) 141, 299
AsuNHI GCTAGC 2 cut(s) 769, 773
AvaII GGWCC 1 cut(s) 694
BaeI ACNNNNGTAYC 2 cut(s) 715, 748
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 432
BbvCI CCTCAGC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 47
BccI CCATC 1 cut(s) 707
BciT130I CCWGG 2 cut(s) 285, 704
BciVI GTATCC 3 cut(s) 308, 511, 743
BclI TGATCA 2 cut(s) 603, 663
BcoDI GTCTC 2 cut(s) 377, 446
BfaI CTAG 3 cut(s) 120, 770, 774
BfrI CTTAAG 1 cut(s) 250
BfuI GTATCC 3 cut(s) 308, 511, 743
BglII AGATCT 1 cut(s) 365
BisI GCNGC 1 cut(s) 61
BlpI GCTNAGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 62
Bme1390I CCNGG 2 cut(s) 285, 704
Bme18I GGWCC 1 cut(s) 694
BmgT120I GGNCC 1 cut(s) 694
BmiI GGNNCC 2 cut(s) 335, 696
BmrFI CCNGG 2 cut(s) 285, 704
BmtI GCTAGC 2 cut(s) 773, 777
BpmI CTGGAG 1 cut(s) 428
Bpu10I CCTNAGC 1 cut(s) 651
Bpu1102I GCTNAGC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 401
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bsc4I CCNNNNNNNGG 1 cut(s) 703
Bse1I ACTGG 1 cut(s) 210
BseBI CCWGG 2 cut(s) 285, 704
BseGI GGATG 1 cut(s) 699
BseLI CCNNNNNNNGG 1 cut(s) 703
BseMII CTCAG 3 cut(s) 231, 427, 642
BseNI ACTGG 1 cut(s) 210
BseRI GAGGAG 1 cut(s) 295
BseXI GCAGC 1 cut(s) 47
BsgI GTGCAG 1 cut(s) 388
BsiHKAI GWGCWC 1 cut(s) 432
BslFI GGGAC 2 cut(s) 630, 680
BslI CCNNNNNNNGG 1 cut(s) 703
BsmAI GTCTC 2 cut(s) 377, 446
BsmFI GGGAC 2 cut(s) 630, 680
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 4 cut(s) 289, 365, 603, 663
Bsp1720I GCTNAGC 1 cut(s) 256
BspCNI CTCAG 3 cut(s) 230, 426, 643
BspLI GGNNCC 2 cut(s) 335, 696
BspOI GCTAGC 2 cut(s) 773, 777
BspTI CTTAAG 1 cut(s) 250
BsrI ACTGG 1 cut(s) 210
BssMI GATC 4 cut(s) 289, 365, 603, 663
Bst2UI CCWGG 2 cut(s) 285, 704
Bst4CI ACNGT 1 cut(s) 621
Bst6I CTCTTC 1 cut(s) 42
BstAFI CTTAAG 1 cut(s) 250
BstC8I GCNNGC 3 cut(s) 456, 771, 775
BstDEI CTNAG 6 cut(s) 217, 256, 293, 371, 413, 651
BstF5I GGATG 1 cut(s) 699
BstKTI GATC 4 cut(s) 292, 368, 606, 666
BstMAI GTCTC 2 cut(s) 377, 446
BstMBI GATC 4 cut(s) 289, 365, 603, 663
BstMWI GCNNNNNNNGC 1 cut(s) 427
BstNI CCWGG 2 cut(s) 285, 704
BstSCI CCNGG 2 cut(s) 283, 702
BstV1I GCAGC 1 cut(s) 47
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BsuI GTATCC 3 cut(s) 308, 511, 743
BtsCI GGATG 1 cut(s) 699
BtsIMutI CAGTG 1 cut(s) 588
Cac8I GCNNGC 3 cut(s) 456, 771, 775
Cfr13I GGNCC 1 cut(s) 694
CseI GACGC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 185
CviAII CATG 2 cut(s) 177, 729
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 6 cut(s) 217, 256, 293, 371, 413, 651
DpnI GATC 4 cut(s) 291, 367, 605, 665
DpnII GATC 4 cut(s) 289, 365, 603, 663
DraI TTTAAA 1 cut(s) 631
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 430
Eco24I GRGCYC 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 694
Eco53kI GAGCTC 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 430
EcoO109I RGGNCCY 1 cut(s) 694
EcoRII CCWGG 2 cut(s) 283, 702
EcoT38I GRGCYC 1 cut(s) 432
FaeI CATG 2 cut(s) 180, 732
FaqI GGGAC 2 cut(s) 630, 680
FatI CATG 2 cut(s) 176, 728
FbaI TGATCA 2 cut(s) 603, 663
Fnu4HI GCNGC 1 cut(s) 61
FokI GGATG 1 cut(s) 686
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 1 cut(s) 61
FspBI CTAG 3 cut(s) 120, 770, 774
GluI GCNGC 1 cut(s) 61
GsuI CTGGAG 1 cut(s) 428
HgaI GACGC 1 cut(s) 65
Hin1II CATG 2 cut(s) 180, 732
HindIII AAGCTT 6 cut(s) 5, 68, 247, 253, 377, 559
HinfI GANTC 2 cut(s) 18, 280
HphI GGTGA 2 cut(s) 141, 299
Hpy166II GTNNAC 1 cut(s) 567
Hpy188I TCNGA 3 cut(s) 12, 345, 416
Hpy188III TCNNGA 4 cut(s) 112, 425, 445, 530
Hpy8I GTNNAC 1 cut(s) 567
HpyAV CCTTC 2 cut(s) 685, 724
HpyCH4III ACNGT 1 cut(s) 621
HpyCH4IV ACGT 1 cut(s) 235
HpyCH4V TGCA 5 cut(s) 95, 267, 405, 458, 537
HpyF10VI GCNNNNNNNGC 1 cut(s) 427
HpyF3I CTNAG 6 cut(s) 217, 256, 293, 371, 413, 651
HpySE526I ACGT 1 cut(s) 235
Hsp92II CATG 2 cut(s) 180, 732
Ksp22I TGATCA 2 cut(s) 603, 663
Kzo9I GATC 4 cut(s) 289, 365, 603, 663
LmnI GCTCC 1 cut(s) 435
Lsp1109I GCAGC 1 cut(s) 47
MaeI CTAG 3 cut(s) 120, 770, 774
MaeII ACGT 1 cut(s) 235
MaeIII GTNAC 1 cut(s) 105
MalI GATC 4 cut(s) 291, 367, 605, 665
MboI GATC 4 cut(s) 289, 365, 603, 663
MboII GAAGA 2 cut(s) 29, 142
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 7 cut(s) 226, 339, 490, 625, 744, 750, 787
MmeI TCCRAC 1 cut(s) 35
MnlI CCTC 6 cut(s) 311, 316, 359, 391, 646, 728
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 3 cut(s) 251, 630, 743
MslI CAYNNNNRTG 1 cut(s) 599
MspCI CTTAAG 1 cut(s) 250
MspR9I CCNGG 2 cut(s) 285, 704
MvaI CCWGG 2 cut(s) 285, 704
MwoI GCNNNNNNNGC 1 cut(s) 427
NdeII GATC 4 cut(s) 289, 365, 603, 663
NheI GCTAGC 2 cut(s) 769, 773
NlaIII CATG 2 cut(s) 180, 732
NlaIV GGNNCC 2 cut(s) 335, 696
NmuCI GTSAC 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 18, 280
PkrI GCNGC 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 694
PsiI TTATAA 1 cut(s) 749
Psp124BI GAGCTC 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 694
Psp6I CCWGG 2 cut(s) 283, 702
PspGI CCWGG 2 cut(s) 283, 702
PspN4I GGNNCC 2 cut(s) 335, 696
PspPI GGNCC 1 cut(s) 694
PspPPI RGGWCCY 1 cut(s) 694
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
RseI CAYNNNNRTG 1 cut(s) 599
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 3 cut(s) 251, 630, 743
SatI GCNGC 1 cut(s) 61
Sau3AI GATC 4 cut(s) 289, 365, 603, 663
Sau96I GGNCC 1 cut(s) 694
ScrFI CCNGG 2 cut(s) 285, 704
SduI GDGCHC 1 cut(s) 432
SinI GGWCC 1 cut(s) 694
SmiMI CAYNNNNRTG 1 cut(s) 599
SmlI CTYRAG 2 cut(s) 250, 380
SmoI CTYRAG 2 cut(s) 250, 380
Sse9I AATT 7 cut(s) 226, 339, 490, 625, 744, 750, 787
SspMI CTAG 3 cut(s) 120, 770, 774
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 2 cut(s) 283, 702
TaaI ACNGT 1 cut(s) 621
TaiI ACGT 1 cut(s) 238
TasI AATT 7 cut(s) 226, 339, 490, 625, 744, 750, 787
TfiI GAWTC 2 cut(s) 18, 280
Tru1I TTAA 3 cut(s) 251, 630, 743
Tru9I TTAA 3 cut(s) 251, 630, 743
TscAI CASTG 1 cut(s) 595
TseFI GTSAC 1 cut(s) 105
TseI GCWGC 1 cut(s) 60
Tsp45I GTSAC 1 cut(s) 105
TspDTI ATGAA 2 cut(s) 17, 143
TspRI CASTG 1 cut(s) 595
Vha464I CTTAAG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 694
XapI RAATTY 2 cut(s) 226, 490
XmnI GAANNNNTTC 1 cut(s) 167
XspI CTAG 3 cut(s) 120, 770, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.