Rroxscaffold_7G00182360

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000654
Physical Location & Seq
Forward (+)
21323079 .. 21327826
4748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00182360.1

Sequence Viewer

Length: 3579 bp
ATGGCTGGTGTTCTGGATACTACTTCCTGCACTTACCGGTGTCGTTATCATGCCTTCTTGAGTTTCAGGGGCGAAGATACACGCAAGGGCTTTAGTGATCATCTCTATAGAGCTTTGGAGTTGGCAGGAATTCACACTTTCAGAGATGATGATGAAATTGAGAGAGGAGCAAATATAGCTACAGAGCTACATAAAGCTATACATGAGTCACGAGTAGCCATAATAGTCTTCTCTAATGACTATGCTTCCTCAAGTTGGTGCTTGGACGAACTTACCAAGATCATGGAACGTAAAAGGACCGATGGATATATGGTAGTGCCAGTTTTCTATAAGGTGGATCCATCTGTTGTTAGGTGGCAGATTAGGAGTTTTGCAGATGCATTTGCCAAACATGAAAAGCGCTTCAAGGAGGAGATCGGCAAGGTGGACAAGTGGAGAAAAGCTCTTAAAGATGTAGCCGATCTAGGAGGGATGGTCTTAGGAGATCAGTACGAATCACAGCTCATCCAAAATATTGTTGACGAGATTGAAAAAAAAATGGATCCCACTACATTAAATGTTGCTCCATATGCTGTTGGAATCAATGCTCGTGTGGAAGACCTCAACATGTGGTTAAGAGATGGATCAGTTGATGTTGGTGTGGCTGGCATCTACGGGATGGGTGGAATTGGCAAGACCACTATTGCTAAAGCTGCTTATAATCTGAACCGGGATAGATTTGAAGGAAGAAGTTTTCTTGCAGATGTTAGAGCAACTACAGAACAACCTAATGGTTTGGTTCGCTTGCAGAGGAACCTTCTTTCAGATATTCAAAGGAGAAAAACAAAAAAGATAAACAGCGTGGATGAAGGTACAAGCAAAATCAAGAATGTTGTATGCTGCAAAAGAGTACTTATTGTTCTTGATGATGTGAATGACTTGGACCAGTTCAATGCGATTCTTGGAATGCGAGAGTGGTTTCATCCAGGAAGCAAGATTATCATAACAACTAGAGATAAGCACTTGCTAACGGCTACTGGGGTTTCTAACATGTTCATGGTTCCAGAATTGGATGAGCATGAATCACTTCAGCTTTTCAGTTGGCATGCCTTCAGACAAGCCCATCCTATCGAAGGCTACATGGAGGTTTCAGCACTCATAGTAAAACACTGTGGAGGGCTACCGTTAGCCCTCCAAGTTCTGGGGTCTTCTCTATTTGGAAAAAGTGTACATGTATGGTACAGTACATTAGAGAAGTTAGATGTGGTTCCTGATAGCAAAATTCAAAGAATTCTTAGAATAAGCTACGATTATCTACAAGATCATCATGACAAAACTATATTTCTTCATATAGCATGTTTCTTCATAGGAAAGAAGAGAAACTTGACATTTACAGTACTGGACAGCCTTGAGTTTTTCACAGCAATTGGAGTTCAAAATCTAGTTGATAGATGTCTACTGCAAATTAATGACGGCAAGTTGATGATGCATCAATTAATCAGAGACATGGGAAGAGGAATTGTTCGCGAAGAATCAGTTGAGGACCCTGGAAAACGTAGTAGAGTGGCGCAAAAAGATGCCTATGATATTTTGAAAAACGCATCGGGCACGCAAACTATCAAGGGTCTCTTGCTCAACCTTTCTATGCTAATGGATGGTATGTCTTCCAAGGCAATACTTAACAATAAACGAGGGCATGCCGAAGAATCAGATGGAAATTGTTCAAGACGGCGTCGACTTAATTTCTTCTCCTGGCAATCAATCAACTATTCCCCAACAAATTTAGCTTCTATATCGAGTGTGGCAGACTTCAAGACAGAGGCATTCAAAAAGATGCCCAATCTTCAACTTCTCCTGCTCAATAATGTAAAAGTCAGTGGTGGCTACGGAGAGTTTCCAAAAACACTAATATGGTTGTCTTGGCAAGGGTTCCCCATGAAATCAATACCAGCCGATTTTTGTTTGAAAAATCTAGTTGCTCTTGAGTTGAAGCATAGCATCCTGCAAGTTGTATGGAAGGGAACCAAGTTTTTTCCAGGATTGAAAATCCTTGATCTCAGTCATTCACAGGGTCTTGTTAGAACCCCAGACTTGTCCGGACTCCCAAACCTTGAGAGATTAATTCTTAAAGATTGCATAAATATTGTCGAGATTGATGAATCCATCGGAGAACTTCAGAAACTTGTATTCTTGAATCTAAAAGACTGCAAAAATCTCTTGAAGCTTCCAAGAAGAATTAGCATGTTGAGATCTCTTCATGAGCTAATCCTTTCTGGCTGCTCAAAGCTTGGTGATACGGAGAAACTCGGTCTTTTATCAAATAAACCATGGCTATCAATCTGTTCGTGGGTATCACCAAGAAAAAGTCTTGAAGCAACCAGCAGCTTCTCGTTGGCATATTTGCCACAGTCTTTAGGAAGCTTAAGTCTGGCTGGTTGCAATCTATCAAAGATTTCCGATGATATTAGTGTTCTGTCCTCGTTAAAGCATCTGGATATAAGTGGCAACCCAATGCTAAGTCTTCCAGAGAGCATAAAGACTCTTTCTATGCTTGACACTCTTTTCTTAGGATACTGCACCAAGCTCAAAATGCTTCCAGAGCTCCCGCCAAGTTTAAGTTCTTTGCATGCAGAAGGTTGTACATCGTTGAGAAAAGTTACGAACTTACCTAACTTTTTCATATCCCTACAACCATTTCTTACGGATTGCAAAGAATTAGCTGAAGTTCAAAGCTTATTCACTATAAAACCATTGAGAAGCATCGACATACAGATGATAAAATATATGGGTCTGTACAATTTGGAATCCACTGAAAGCATTGAAGTCGAAATGAGCAATCACCTGACAGATACAAACAAGACAGGTCCACTCCAGGGACTCTATGAATGTGGAATATTTAGCATTTTCCTTCATGGAAGCACAATTCCTGACTGCTACACCTACAGAAGCATGGGTGATTCAGTGTTGTCTATAACTGTTCCTTCCCATCTTAAGCTTAAGATCCGAGGCTTAAATGTATGTATTCTATATGCACTAGGCCCATGCCAGCACCTTAAAGTCAGTAATGACACGAAGGGTCTTATGTGGACATACTGCCCAGTCACTGTAGGTGTTCCTAAAGAGGATGGACTGATGCTATGGCTAAGTCATTGGCTGTTTGAGAACCATGAGTTGGAGGCTGGGGATGAATTACGTGTCTCGGTGCATAATGGAGCTATATTCTTAGACCCTCTTGGTCGTCAAGACGTTCTTCCAGCAAAGGAATTTGGAATCCAACTTGTGTATGAATCGGAAAATAAGGACGTCCGATCCCTAAGTGAAGATCTTGCGGTACAACATGAAGCGCCTTATTGGAGTTACGATATTGTTACTGGGAATGGATCATTGTCAGCATCAAAGTATCAAATGTGGACAGGCAAATACTTTCTGTGCAATTATATTCACCATATGCGTCAGCTTTATCTCAGAAATTGCGAGGAGAATCCAGCTCATCTTGATTTTTCATATGAGCCCAAAGATCGTTTGAGTGGATATTGCCGTCATTTATTTGAGCACACTCACTTTCACAAGAAAAAATTTGAGTTTGTATTGAATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1192

Amino Acids

134.82

Weight (kDa)

7.87

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 184 2.3e-51 TIR domain
TIR_2 PF13676 19 - 128 1.1e-16 TIR domain
NB-ARC PF00931 197 - 366 1e-20 NB-ARC domain
WHD_ROQ1 PF23282 435 - 505 2e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 670 - 884 1.8e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 801 - 919 2.5e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 699
AatII GACGTC 1 cut(s) 3285
AccB7I CCANNNNNTGG 2 cut(s) 1180, 3151
AccI GTMKAC 2 cut(s) 1435, 1714
AccII CGCG 1 cut(s) 1506
AccIII TCCGGA 1 cut(s) 2075
AciI CCGC 2 cut(s) 2585, 3308
AclWI GGATC 8 cut(s) 332, 345, 536, 549, 631, 2974, 3282, 3367
AcsI RAATTY 6 cut(s) 129, 1260, 1269, 1759, 3242, 3554
AcuI CTGAAG 4 cut(s) 1052, 1075, 2138, 2721
AcyI GRCGYC 2 cut(s) 1711, 3282
AfeI AGCGCT 1 cut(s) 401
AfiI CCNNNNNNNGG 5 cut(s) 255, 1112, 1180, 2852, 3151
AflII CTTAAG 3 cut(s) 2401, 2969, 2975
AflIII ACRYGT 4 cut(s) 606, 1029, 1210, 3172
AgeI ACCGGT 1 cut(s) 36
AjnI CCWGG 5 cut(s) 964, 1525, 1730, 2014, 2850
AjuI GAANNNNNNNTTGG 4 cut(s) 2581, 2613, 3134, 3166
Alw21I GWGCWC 2 cut(s) 2583, 3534
Alw26I GTCTC 3 cut(s) 1476, 1610, 3181
AlwI GGATC 8 cut(s) 332, 345, 536, 549, 631, 2974, 3282, 3367
AlwNI CAGNNNCTG 1 cut(s) 3083
Aor13HI TCCGGA 1 cut(s) 2075
Aor51HI AGCGCT 1 cut(s) 401
AoxI GGCC 1 cut(s) 3016
ApeKI GCWGC 4 cut(s) 692, 879, 2256, 2361
ApoI RAATTY 6 cut(s) 129, 1260, 1269, 1759, 3242, 3554
AseI ATTAAT 3 cut(s) 1446, 1475, 2099
AsiGI ACCGGT 1 cut(s) 36
Asp700I GAANNNNTTC 2 cut(s) 1065, 1699
AspLEI GCGC 3 cut(s) 402, 1549, 3325
AspS9I GGNCC 5 cut(s) 297, 922, 1522, 2843, 3017
AsuC2I CCSGG 1 cut(s) 710
AsuHPI GGTGA 5 cut(s) 2282, 2325, 2810, 2945, 3413
AvaII GGWCC 4 cut(s) 297, 922, 1522, 2843
BaeGI GKGCMC 1 cut(s) 1589
BamHI GGATCC 2 cut(s) 337, 541
BanII GRGCYC 2 cut(s) 2583, 3492
BauI CACGAG 2 cut(s) 210, 588
BbsI GAAGAC 5 cut(s) 220, 603, 1179, 1635, 2492
Bbv12I GWGCWC 2 cut(s) 2583, 3534
BbvI GCAGC 4 cut(s) 679, 866, 2243, 2373
BceAI ACGGC 4 cut(s) 1026, 1468, 1724, 3501
BcgI CGANNNNNNTGC 2 cut(s) 2785, 2819
BciT130I CCWGG 5 cut(s) 966, 1527, 1732, 2016, 2852
BciVI GTATCC 2 cut(s) 10, 2543
BclI TGATCA 1 cut(s) 97
BcnI CCSGG 1 cut(s) 710
BcoDI GTCTC 3 cut(s) 1476, 1610, 3181
BfaI CTAG 5 cut(s) 464, 990, 1421, 1952, 3014
BfmI CTRYAG 5 cut(s) 106, 180, 756, 2920, 3084
BfoI RGCGCY 2 cut(s) 403, 3326
BfrI CTTAAG 3 cut(s) 2401, 2969, 2975
BfuI GTATCC 2 cut(s) 10, 2543
BglII AGATCT 2 cut(s) 2228, 3301
BisI GCNGC 4 cut(s) 693, 880, 2257, 2362
BlsI GCNGC 4 cut(s) 694, 881, 2258, 2363
BmcAI AGTACT 2 cut(s) 891, 1377
Bme1390I CCNGG 6 cut(s) 710, 966, 1527, 1732, 2016, 2852
Bme18I GGWCC 4 cut(s) 297, 922, 1522, 2843
BmgT120I GGNCC 5 cut(s) 297, 922, 1522, 2843, 3017
BmiI GGNNCC 8 cut(s) 339, 543, 794, 1041, 1248, 1524, 1910, 2002
BmrFI CCNGG 6 cut(s) 710, 966, 1527, 1732, 2016, 2852
BmrI ACTGGG 3 cut(s) 1026, 3071, 3360
BmuI ACTGGG 3 cut(s) 1026, 3071, 3360
BpiI GAAGAC 5 cut(s) 220, 603, 1179, 1635, 2492
BplI GAGNNNNNCTC 2 cut(s) 427, 459
BpmI CTGGAG 1 cut(s) 2834
BpuEI CTTGAG 5 cut(s) 79, 235, 1409, 1982, 2111
BpuMI CCSGG 1 cut(s) 710
BsaAI YACGTR 1 cut(s) 3173
BsaHI GRCGYC 2 cut(s) 1711, 3282
BsaI GGTCTC 1 cut(s) 1610
BsaJI CCNNGG 5 cut(s) 1525, 1647, 2306, 2851, 2983
BsaWI WCCGGW 2 cut(s) 36, 2075
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 1112, 1180, 2852, 3151
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 6 cut(s) 320, 925, 1021, 1383, 3077, 3355
BseAI TCCGGA 1 cut(s) 2075
BseBI CCWGG 5 cut(s) 966, 1527, 1732, 2016, 2852
BseDI CCNNGG 5 cut(s) 1525, 1647, 2306, 2851, 2983
BseLI CCNNNNNNNGG 5 cut(s) 255, 1112, 1180, 2852, 3151
BseMII CTCAG 2 cut(s) 2050, 3457
BseNI ACTGG 6 cut(s) 320, 925, 1021, 1383, 3077, 3355
BseRI GAGGAG 3 cut(s) 180, 425, 3470
BseSI GKGCMC 1 cut(s) 1589
BseXI GCAGC 4 cut(s) 679, 866, 2243, 2373
BseYI CCCAGC 1 cut(s) 3158
BsgI GTGCAG 2 cut(s) 13, 2539
Bsh1236I CGCG 1 cut(s) 1506
BshFI GGCC 1 cut(s) 3018
BshTI ACCGGT 1 cut(s) 36
BsiHKAI GWGCWC 2 cut(s) 2583, 3534
BsiSI CCGG 3 cut(s) 37, 709, 2076
BslFI GGGAC 1 cut(s) 2868
BslI CCNNNNNNNGG 5 cut(s) 255, 1112, 1180, 2852, 3151
BsmAI GTCTC 3 cut(s) 1476, 1610, 3181
BsmFI GGGAC 1 cut(s) 2868
BsmI GAATGC 2 cut(s) 951, 1802
BsnI GGCC 1 cut(s) 3018
Bso31I GGTCTC 1 cut(s) 1610
Bsp1286I GDGCHC 4 cut(s) 1589, 2583, 3492, 3534
Bsp13I TCCGGA 1 cut(s) 2075
Bsp1407I TGTACA 3 cut(s) 1207, 2618, 2772
Bsp19I CCATGG 1 cut(s) 2306
Bsp68I TCGCGA 1 cut(s) 1506
BspACI CCGC 2 cut(s) 2585, 3308
BspANI GGCC 1 cut(s) 3018
BspCNI CTCAG 2 cut(s) 2049, 3456
BspEI TCCGGA 1 cut(s) 2075
BspFNI CGCG 1 cut(s) 1506
BspHI TCATGA 2 cut(s) 1306, 2236
BspLI GGNNCC 8 cut(s) 339, 543, 794, 1041, 1248, 1524, 1910, 2002
BspPI GGATC 8 cut(s) 332, 345, 536, 549, 631, 2974, 3282, 3367
BspTI CTTAAG 3 cut(s) 2401, 2969, 2975
BspTNI GGTCTC 1 cut(s) 1610
BsrFI RCCGGY 1 cut(s) 36
BsrGI TGTACA 3 cut(s) 1207, 2618, 2772
BsrI ACTGG 6 cut(s) 320, 925, 1021, 1383, 3077, 3355
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 5 cut(s) 1525, 1647, 2306, 2851, 2983
BssNI GRCGYC 2 cut(s) 1711, 3282
BssSI CACGAG 2 cut(s) 210, 588
BssT1I CCWWGG 2 cut(s) 1647, 2306
Bst2BI CACGAG 2 cut(s) 210, 588
Bst2UI CCWGG 5 cut(s) 966, 1527, 1732, 2016, 2852
Bst4CI ACNGT 7 cut(s) 1151, 1164, 1223, 1375, 2388, 2956, 3085
Bst6I CTCTTC 3 cut(s) 1349, 1486, 2238
BstACI GRCGYC 2 cut(s) 1711, 3282
BstAFI CTTAAG 3 cut(s) 2401, 2969, 2975
BstAUI TGTACA 3 cut(s) 1207, 2618, 2772
BstBAI YACGTR 1 cut(s) 3173
BstC8I GCNNGC 7 cut(s) 646, 785, 1086, 1589, 1677, 2607, 3026
BstDEI CTNAG 9 cut(s) 478, 1274, 2036, 2495, 2545, 3122, 3202, 3293, 3443
BstDSI CCRYGG 1 cut(s) 2306
BstENI CCTNNNNNAGG 1 cut(s) 1110
BstFNI CGCG 1 cut(s) 1506
BstH2I RGCGCY 2 cut(s) 403, 3326
BstHHI GCGC 3 cut(s) 402, 1549, 3325
BstMAI GTCTC 3 cut(s) 1476, 1610, 3181
BstMWI GCNNNNNNNGC 5 cut(s) 176, 569, 692, 2569, 2578
BstNI CCWGG 5 cut(s) 966, 1527, 1732, 2016, 2852
BstNSI RCATGY 8 cut(s) 610, 1033, 1088, 1214, 1338, 1679, 2224, 2609
BstSCI CCNGG 6 cut(s) 708, 964, 1525, 1730, 2014, 2850
BstSFI CTRYAG 5 cut(s) 106, 180, 756, 2920, 3084
BstSLI GKGCMC 1 cut(s) 1589
BstUI CGCG 1 cut(s) 1506
BstV1I GCAGC 4 cut(s) 679, 866, 2243, 2373
BstV2I GAAGAC 5 cut(s) 220, 603, 1179, 1635, 2492
BstX2I RGATCY 5 cut(s) 337, 541, 2228, 2979, 3301
BstXI CCANNNNNNTGG 1 cut(s) 283
BstYI RGATCY 5 cut(s) 337, 541, 2228, 2979, 3301
BsuI GTATCC 2 cut(s) 10, 2543
BsuRI GGCC 1 cut(s) 3018
BtgI CCRYGG 1 cut(s) 2306
BtsIMutI CAGTG 5 cut(s) 1147, 1861, 2787, 2946, 3081
BtuMI TCGCGA 1 cut(s) 1506
Cac8I GCNNGC 7 cut(s) 646, 785, 1086, 1589, 1677, 2607, 3026
CaiI CAGNNNCTG 1 cut(s) 3083
CciI TCATGA 2 cut(s) 1306, 2236
CfoI GCGC 3 cut(s) 402, 1549, 3325
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 5 cut(s) 297, 922, 1522, 2843, 3017
CseI GACGC 2 cut(s) 1700, 3419
CspAI ACCGGT 1 cut(s) 36
DdeI CTNAG 9 cut(s) 478, 1274, 2036, 2495, 2545, 3122, 3202, 3293, 3443
Eam1104I CTCTTC 3 cut(s) 1349, 1486, 2238
EarI CTCTTC 3 cut(s) 1349, 1486, 2238
Ecl136II GAGCTC 1 cut(s) 2581
Eco130I CCWWGG 2 cut(s) 1647, 2306
Eco24I GRGCYC 2 cut(s) 2583, 3492
Eco31I GGTCTC 1 cut(s) 1610
Eco47I GGWCC 4 cut(s) 297, 922, 1522, 2843
Eco47III AGCGCT 1 cut(s) 401
Eco53kI GAGCTC 1 cut(s) 2581
Eco57I CTGAAG 4 cut(s) 1052, 1075, 2138, 2721
EcoICRI GAGCTC 1 cut(s) 2581
EcoNI CCTNNNNNAGG 1 cut(s) 1110
EcoO109I RGGNCCY 1 cut(s) 1522
EcoRI GAATTC 2 cut(s) 129, 1269
EcoRII CCWGG 5 cut(s) 964, 1525, 1730, 2014, 2850
EcoT14I CCWWGG 2 cut(s) 1647, 2306
EcoT22I ATGCAT 2 cut(s) 382, 1470
EcoT38I GRGCYC 2 cut(s) 2583, 3492
ErhI CCWWGG 2 cut(s) 1647, 2306
FalI AAGNNNNNCTT 6 cut(s) 1346, 1378, 1592, 1624, 3213, 3245
FaqI GGGAC 1 cut(s) 2868
FauI CCCGC 1 cut(s) 2592
FauNDI CATATG 3 cut(s) 568, 3426, 3484
FbaI TGATCA 1 cut(s) 97
FblI GTMKAC 2 cut(s) 1435, 1714
Fnu4HI GCNGC 4 cut(s) 693, 880, 2257, 2362
FriOI GRGCYC 2 cut(s) 2583, 3492
Fsp4HI GCNGC 4 cut(s) 693, 880, 2257, 2362
FspBI CTAG 5 cut(s) 464, 990, 1421, 1952, 3014
GlaI GCGC 3 cut(s) 401, 1548, 3324
GluI GCNGC 4 cut(s) 693, 880, 2257, 2362
GsaI CCCAGC 1 cut(s) 3162
GsuI CTGGAG 1 cut(s) 2834
HaeII RGCGCY 2 cut(s) 403, 3326
HaeIII GGCC 1 cut(s) 3018
HapII CCGG 3 cut(s) 37, 709, 2076
HgaI GACGC 2 cut(s) 1700, 3419
HhaI GCGC 3 cut(s) 402, 1549, 3325
Hin1I GRCGYC 2 cut(s) 1711, 3282
Hin6I GCGC 3 cut(s) 400, 1547, 3323
HinP1I GCGC 3 cut(s) 400, 1547, 3323
HincII GTYRAC 2 cut(s) 520, 1715
HindII GTYRAC 2 cut(s) 520, 1715
HindIII AAGCTT 5 cut(s) 2201, 2264, 2398, 2710, 2972
HpaII CCGG 3 cut(s) 37, 709, 2076
HphI GGTGA 5 cut(s) 2282, 2325, 2810, 2945, 3413
Hpy166II GTNNAC 8 cut(s) 427, 520, 1208, 1436, 1715, 2846, 3066, 3390
Hpy8I GTNNAC 8 cut(s) 427, 520, 1208, 1436, 1715, 2846, 3066, 3390
Hpy99I CGWCG 1 cut(s) 1716
HpyCH4III ACNGT 7 cut(s) 1151, 1164, 1223, 1375, 2388, 2956, 3085
HpyCH4IV ACGT 5 cut(s) 289, 1534, 3172, 3225, 3282
HpyF10VI GCNNNNNNNGC 5 cut(s) 176, 569, 692, 2569, 2578
HpyF3I CTNAG 9 cut(s) 478, 1274, 2036, 2495, 2545, 3122, 3202, 3293, 3443
HpySE526I ACGT 5 cut(s) 289, 1534, 3172, 3225, 3282
Hsp92I GRCGYC 2 cut(s) 1711, 3282
HspAI GCGC 3 cut(s) 400, 1547, 3323
Kpn2I TCCGGA 1 cut(s) 2075
Ksp22I TGATCA 1 cut(s) 97
LmnI GCTCC 4 cut(s) 167, 568, 2586, 3191
Lsp1109I GCAGC 4 cut(s) 679, 866, 2243, 2373
MaeI CTAG 5 cut(s) 464, 990, 1421, 1952, 3014
MaeII ACGT 5 cut(s) 289, 1534, 3172, 3225, 3282
MaeIII GTNAC 5 cut(s) 207, 2635, 3079, 3335, 3346
MfeI CAATTG 1 cut(s) 1404
MflI RGATCY 5 cut(s) 337, 541, 2228, 2979, 3301
MhlI GDGCHC 4 cut(s) 1589, 2583, 3492, 3534
MlyI GAGTC 4 cut(s) 215, 2073, 2512, 2850
MmeI TCCRAC 3 cut(s) 556, 3132, 3277
Mph1103I ATGCAT 2 cut(s) 382, 1470
MroI TCCGGA 1 cut(s) 2075
MroXI GAANNNNTTC 2 cut(s) 1065, 1699
MslI CAYNNNNRTG 3 cut(s) 1034, 1888, 2750
MspCI CTTAAG 3 cut(s) 2401, 2969, 2975
MspI CCGG 3 cut(s) 37, 709, 2076
MspR9I CCNGG 6 cut(s) 710, 966, 1527, 1732, 2016, 2852
MunI CAATTG 1 cut(s) 1404
Mva1269I GAATGC 2 cut(s) 951, 1802
MvaI CCWGG 5 cut(s) 966, 1527, 1732, 2016, 2852
MvnI CGCG 1 cut(s) 1506
MwoI GCNNNNNNNGC 5 cut(s) 176, 569, 692, 2569, 2578
NciI CCSGG 1 cut(s) 710
NcoI CCATGG 1 cut(s) 2306
NdeI CATATG 3 cut(s) 568, 3426, 3484
NlaIV GGNNCC 8 cut(s) 339, 543, 794, 1041, 1248, 1524, 1910, 2002
NmuCI GTSAC 2 cut(s) 207, 3079
NruI TCGCGA 1 cut(s) 1506
NsiI ATGCAT 2 cut(s) 382, 1470
NspI RCATGY 8 cut(s) 610, 1033, 1088, 1214, 1338, 1679, 2224, 2609
PaeI GCATGC 3 cut(s) 1088, 1679, 2609
PagI TCATGA 2 cut(s) 1306, 2236
PciI ACATGT 3 cut(s) 606, 1029, 1210
PctI GAATGC 2 cut(s) 951, 1802
PdmI GAANNNNTTC 2 cut(s) 1065, 1699
PflFI GACNNNGTC 1 cut(s) 1710
PflMI CCANNNNNTGG 2 cut(s) 1180, 3151
PfoI TCCNGGA 2 cut(s) 964, 2014
PinAI ACCGGT 1 cut(s) 36
PkrI GCNGC 4 cut(s) 694, 881, 2258, 2363
PleI GAGTC 4 cut(s) 214, 2073, 2512, 2850
PpsI GAGTC 4 cut(s) 214, 2073, 2512, 2850
Ppu21I YACGTR 1 cut(s) 3173
PpuMI RGGWCCY 1 cut(s) 1522
PscI ACATGT 3 cut(s) 606, 1029, 1210
PshBI ATTAAT 3 cut(s) 1446, 1475, 2099
PsiI TTATAA 1 cut(s) 699
Psp124BI GAGCTC 1 cut(s) 2583
Psp5II RGGWCCY 1 cut(s) 1522
Psp6I CCWGG 5 cut(s) 964, 1525, 1730, 2014, 2850
PspFI CCCAGC 1 cut(s) 3158
PspGI CCWGG 5 cut(s) 964, 1525, 1730, 2014, 2850
PspN4I GGNNCC 8 cut(s) 339, 543, 794, 1041, 1248, 1524, 1910, 2002
PspPI GGNCC 5 cut(s) 297, 922, 1522, 2843, 3017
PspPPI RGGWCCY 1 cut(s) 1522
PsrI GAACNNNNNNTAC 2 cut(s) 882, 914
PstNI CAGNNNCTG 1 cut(s) 3083
PsuI RGATCY 5 cut(s) 337, 541, 2228, 2979, 3301
PsyI GACNNNGTC 1 cut(s) 1710
RruI TCGCGA 1 cut(s) 1506
RseI CAYNNNNRTG 3 cut(s) 1034, 1888, 2750
SacI GAGCTC 1 cut(s) 2583
SalI GTCGAC 1 cut(s) 1713
SatI GCNGC 4 cut(s) 693, 880, 2257, 2362
Sau96I GGNCC 5 cut(s) 297, 922, 1522, 2843, 3017
ScaI AGTACT 2 cut(s) 891, 1377
SchI GAGTC 4 cut(s) 215, 2073, 2512, 2850
ScrFI CCNGG 6 cut(s) 710, 966, 1527, 1732, 2016, 2852
SduI GDGCHC 4 cut(s) 1589, 2583, 3492, 3534
SfcI CTRYAG 5 cut(s) 106, 180, 756, 2920, 3084
SinI GGWCC 4 cut(s) 297, 922, 1522, 2843
SmiMI CAYNNNNRTG 3 cut(s) 1034, 1888, 2750
SmlI CTYRAG 8 cut(s) 58, 250, 1388, 1961, 2090, 2401, 2969, 2975
SmoI CTYRAG 8 cut(s) 58, 250, 1388, 1961, 2090, 2401, 2969, 2975
SphI GCATGC 3 cut(s) 1088, 1679, 2609
SsiI CCGC 2 cut(s) 2585, 3308
SspI AATATT 3 cut(s) 514, 2122, 2874
SspMI CTAG 5 cut(s) 464, 990, 1421, 1952, 3014
SstI GAGCTC 1 cut(s) 2583
StyD4I CCNGG 6 cut(s) 708, 964, 1525, 1730, 2014, 2850
StyI CCWWGG 2 cut(s) 1647, 2306
TaaI ACNGT 7 cut(s) 1151, 1164, 1223, 1375, 2388, 2956, 3085
TaiI ACGT 5 cut(s) 292, 1537, 3175, 3228, 3285
TaqI TCGA 6 cut(s) 1110, 1714, 1775, 2127, 2742, 2805
TaqII GACCGA 2 cut(s) 314, 2276
TatI WGTACW 6 cut(s) 889, 1207, 1223, 1375, 2618, 2772
TscAI CASTG 5 cut(s) 1154, 1861, 2794, 2946, 3088
TseFI GTSAC 2 cut(s) 207, 3079
TseI GCWGC 4 cut(s) 692, 879, 2256, 2361
Tsp45I GTSAC 2 cut(s) 207, 3079
TspGWI ACGGA 3 cut(s) 1881, 2291, 2696
TspRI CASTG 5 cut(s) 1154, 1861, 2794, 2946, 3088
Tth111I GACNNNGTC 1 cut(s) 1710
Van91I CCANNNNNTGG 2 cut(s) 1180, 3151
Vha464I CTTAAG 3 cut(s) 2401, 2969, 2975
VpaK11BI GGWCC 4 cut(s) 297, 922, 1522, 2843
VspI ATTAAT 3 cut(s) 1446, 1475, 2099
XagI CCTNNNNNAGG 1 cut(s) 1110
XapI RAATTY 6 cut(s) 129, 1260, 1269, 1759, 3242, 3554
XceI RCATGY 8 cut(s) 610, 1033, 1088, 1214, 1338, 1679, 2224, 2609
XmiI GTMKAC 2 cut(s) 1435, 1714
XmnI GAANNNNTTC 2 cut(s) 1065, 1699
XspI CTAG 5 cut(s) 464, 990, 1421, 1952, 3014
ZraI GACGTC 1 cut(s) 3283
ZrmI AGTACT 2 cut(s) 891, 1377
Zsp2I ATGCAT 2 cut(s) 382, 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.