Prupe.3G010300_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000654
Physical Location & Seq
Forward (+)
653401 .. 656614
3214 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G010300.1

Sequence Viewer

Length: 2472 bp
ATGAACCTTTCAAGATCTGTAGTACAACACTGTGAAGGCCTTCCACTAGCTCTCCAAGTTTTGGGATCTTCTCTATTTGGAAAAAGTGTAGATCTATGGCAAAGTGCATTACAGAAGTTGCACGTGATTCCTGATGACAAGATTCAAAAGATTCTTAGAATAAGCTTTGATTCTCTAAAAGATGATCATGACAGGAATTTATTCCTTCATATTGTCTGTTTCTTCATAGAAAAGAAGATGGGTTATACAATTACAGTACTAGACAATTTAAATTTTTACACAAGGATTGGAATTCAAAATCTAGTTGATAGATGTCTAGTGGAAATTGATATAGACAACAGGTTGATCGTCTATCAATTACTTAGAGACATGGGAAGGGCAATTATTCGTGAAGAATCACCCGAGGACCCTGGAAAGCGTAGTAGAGTGTGGCATAAAGATTCCTCTGACGTTTTGAGAAAATTAACTCAGGGAACAGAAACTATTAAGGGCCTCATGCTCAACCTTCCAAGCGAGGCAATATTTAGTACAAGTAACCAAAAGCGGCGCCATGTTGAAGACTTTGATGGAAATTGTTCGAGACGACGTCGACTTGGTTACTCTTGGATATCTATTAACTCTTCCTCAACAAATTCAACTGCTGCATCAAATGAGGTGGACTTCAAGGCAGAGGCATTTAGAAGAATGCACAATCTTGAACTGCTCCTGCTCGAAAATGTAAAAGTCAGTGGAGATTATGAAGATTTTCCAAAAAAATTAATATGGTTGTGCTGGCGAGGATTCCCTTTAAAATCAATACCAGAAAAATTTTATTTGGAAAATCTAGTTGGTCTTGACTTGCGAAATAGCAACCTACAACATGTTTGGAAGGGAACCAGGTTTCTTCTAGGACTGAAAATTCTTAATCTCAGTCATTCGCATAGCCTTGTGACAACCCCCGACTTGTCAGGAGTCCCTAACCTTGAGAAATTGATTCTTAAAGACTGCATAAATTTGGTTGTGATTGATGAATCCCTTGGAAACCTAGAGAAACTCATCTTCTTGAATCTAAAAGACTGCAGAAGTCTCATGAAGCTTCCAACAAGAATTACCATGTTGAGATCTCTTCAGGAACTTGATCTTTCTGGGTGCTCAAAGCTTGTGCTTCATACCAGTACGACTGCTGCTAATCACTTACACTTTACAACTAGAGTTAGGAAGAAATTAAATATGTTATCAGAAAAAATATGGCAATCGACATGGTCATGGAGGTCATGGGTATCAACAAGAAATAAGCTTGAATTAGCCAGTTTGTCCATGGAAATCTGGCCAAATTGCTTAGGAACCTTAAGTCTGGCTGACTGCAATCTGTCAGAGATTCCTGGTGATCTTAGTATCTTGTCCTTGTTGAAGCATTTGAATCTAAGTAGAAACCCAATTCTGAGGCTACCAGAAAACATGAACGGTCTTATTATGCTCCAGACTCTTGAGATACAAGGTTGCGCAAAGCTACGCACTCTTCCAAAGCTCCCACGGAGTCTAAGAAAATTGCATGCAAGTTATTGTACATCACTGGAAAGAATAACGAGTCTACCAAACATGTTTGAATCATTGGATTCAAGTCTTTGGAAATGCAAGAAATTAGTTGAAGTTCAAAGCTTGTTCGATATAAAACCGTTGAAAAGGGTTGACATAGAAATGATCAGGGATATGGGCCTGTTTAATTTGGAATCCACAGGCGTAGGTACTGAGGTTGAAATGACCAATTACCTGACATGTACAACAAGGAACGGCCCCCTCCAGGGACTCTATGAATGTGGCATATTTAGTATTTTCGTTCATGGAAACAAGATTCCAGATCGGTTCACATACAGGAGCATGGGTAAATCTGTTTTGTCTATCATTCTACCTTCACATTTTAATCTCAAGATCCGAGGCTTAAATATATGTGTTATGCATTCACGCTGCCCATTTTGGTTTAGTAGTAGAAACTTTCTTAAAGTCAGTAATGAGACCAAAGGTCTTAAGTGGACCTATTGCCCAGTTGCAGCAGGTCTCCCAAAAAAGAACCAAGACATGCTATGGCTAAGCCACTGGCGGTTTGAAAATGATGAATTGGAGGAAGGAGAACAAGTACATGTTTCAATAAATGAGGAATTTAGTTTCTGGGCAAAGGAGTTCTGTATCCAGCTTGTGTACGAGAAGGACCCATCAAATAGTGAAGATATAATAATCCAACAGGAAACACCTCCTTCGAGCCAGATTGCTGCTGTTGGAAATGTATCAGCATCAGCGTCCAAGTATCAATTCTGGACGGGCAAATACTTTCTATGTAACCATCGGGCTCGTATTCATCAACATCAATTCTCTAGGAGGCAGACGAACCCATCTTATCTTGAACATTACAAGCCCGAGACGGATACCTTTCATTACTTATTTGATCAAGATGTTCATCCTCAGGTCAATCATTCCATCTATTCGGAAGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

824

Amino Acids

94.87

Weight (kDa)

8.7

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 589
Acc16I TGCGCA 1 cut(s) 1483
Acc36I ACCTGC 1 cut(s) 2021
AccB1I GGYRCC 1 cut(s) 546
AccB7I CCANNNNNTGG 1 cut(s) 61
AccI GTMKAC 2 cut(s) 589, 1570
AciI CCGC 2 cut(s) 544, 2077
AclWI GGATC 2 cut(s) 73, 1903
AcoI YGGCCR 1 cut(s) 1307
AcsI RAATTY 8 cut(s) 196, 271, 291, 631, 806, 897, 991, 2135
AcuI CTGAAG 1 cut(s) 1091
AcvI CACGTG 1 cut(s) 124
AcyI GRCGYC 2 cut(s) 547, 586
AfaI GTAC 9 cut(s) 24, 258, 529, 1156, 1546, 1726, 1759, 2115, 2177
AfiI CCNNNNNNNGG 3 cut(s) 61, 1780, 1781
AflII CTTAAG 2 cut(s) 1327, 2003
AflIII ACRYGT 4 cut(s) 859, 1578, 1754, 2116
AhdI GACNNNNNGTC 1 cut(s) 1998
AjnI CCWGG 4 cut(s) 409, 875, 1360, 1779
AjuI GAANNNNNNNTTGG 4 cut(s) 810, 842, 2270, 2302
AluBI AGCT 9 cut(s) 50, 165, 1075, 1138, 1276, 1489, 1507, 1638, 2170
AluI AGCT 9 cut(s) 50, 165, 1075, 1138, 1276, 1489, 1507, 1638, 2170
Alw21I GWGCWC 1 cut(s) 1133
Alw26I GTCTC 6 cut(s) 360, 574, 1070, 1985, 2039, 2387
AlwI GGATC 2 cut(s) 73, 1903
Ama87I CYCGRG 2 cut(s) 401, 2390
AoxI GGCC 5 cut(s) 37, 490, 1307, 1693, 1771
ApeKI GCWGC 5 cut(s) 641, 1163, 1944, 2027, 2246
ApoI RAATTY 8 cut(s) 196, 271, 291, 631, 806, 897, 991, 2135
AseI ATTAAT 1 cut(s) 758
Asp700I GAANNNNTTC 4 cut(s) 39, 200, 574, 744
AspLEI GCGC 2 cut(s) 549, 1484
AspS9I GGNCC 6 cut(s) 406, 490, 1693, 1772, 2010, 2185
AsuHPI GGTGA 2 cut(s) 390, 1376
AvaI CYCGRG 2 cut(s) 401, 2390
AvaII GGWCC 3 cut(s) 406, 2010, 2185
AxyI CCTNAGG 1 cut(s) 2436
BaeI ACNNNNGTAYC 2 cut(s) 1716, 1749
BalI TGGCCA 1 cut(s) 1309
BanI GGYRCC 1 cut(s) 546
BanII GRGCYC 1 cut(s) 2326
BbrPI CACGTG 1 cut(s) 124
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 1133
BbvI GCAGC 5 cut(s) 628, 1150, 1931, 2039, 2233
BccI CCATC 6 cut(s) 232, 560, 2197, 2325, 2374, 2459
BceAI ACGGC 1 cut(s) 1786
BciT130I CCWGG 4 cut(s) 411, 877, 1362, 1781
BciVI GTATCC 2 cut(s) 2174, 2392
BclI TGATCA 3 cut(s) 184, 1680, 2419
BcoDI GTCTC 6 cut(s) 360, 574, 1070, 1985, 2039, 2387
BfaI CTAG 9 cut(s) 47, 260, 302, 317, 824, 887, 1025, 1188, 2349
BfmI CTRYAG 2 cut(s) 18, 1057
BfoI RGCGCY 1 cut(s) 550
BfrI CTTAAG 2 cut(s) 1327, 2003
BfuAI ACCTGC 1 cut(s) 2021
BfuI GTATCC 2 cut(s) 2174, 2392
BglII AGATCT 3 cut(s) 14, 91, 1100
BisI GCNGC 6 cut(s) 545, 642, 1164, 1945, 2028, 2247
BlpI GCTNAGC 1 cut(s) 2066
BlsI GCNGC 6 cut(s) 546, 643, 1165, 1946, 2029, 2248
BmcAI AGTACT 1 cut(s) 258
Bme1390I CCNGG 4 cut(s) 411, 877, 1362, 1781
Bme18I GGWCC 3 cut(s) 406, 2010, 2185
BmeRI GACNNNNNGTC 1 cut(s) 1998
BmeT110I CYCGRG 2 cut(s) 401, 2390
BmgT120I GGNCC 6 cut(s) 406, 490, 1693, 1772, 2010, 2185
BmiI GGNNCC 6 cut(s) 408, 548, 874, 1324, 1774, 2187
BmrFI CCNGG 4 cut(s) 411, 877, 1362, 1781
BmrI ACTGGG 1 cut(s) 2015
BmsI GCATC 2 cut(s) 653, 2276
BmuI ACTGGG 1 cut(s) 2015
BpiI GAAGAC 1 cut(s) 564
BpmI CTGGAG 2 cut(s) 1442, 1763
Bpu10I CCTNAGC 1 cut(s) 1318
Bpu1102I GCTNAGC 1 cut(s) 2066
BpuEI CTTGAG 3 cut(s) 983, 1487, 1889
BsaAI YACGTR 1 cut(s) 124
BsaBI GATNNNNATC 1 cut(s) 2430
BsaHI GRCGYC 2 cut(s) 547, 586
BsaI GGTCTC 2 cut(s) 1985, 2039
BsaJI CCNNGG 7 cut(s) 402, 409, 1015, 1296, 1511, 1780, 1912
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 1846, 1876, 2097, 2127
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 1780, 1781
Bse1I ACTGG 5 cut(s) 1152, 1287, 1557, 2021, 2078
Bse21I CCTNAGG 1 cut(s) 2436
Bse8I GATNNNNATC 1 cut(s) 2430
BseBI CCWGG 4 cut(s) 411, 877, 1362, 1781
BseDI CCNNGG 7 cut(s) 402, 409, 1015, 1296, 1511, 1780, 1912
BseGI GGATG 1 cut(s) 2431
BseJI GATNNNNATC 1 cut(s) 2430
BseLI CCNNNNNNNGG 3 cut(s) 61, 1780, 1781
BseMII CTCAG 5 cut(s) 482, 922, 1412, 1719, 2450
BseNI ACTGG 5 cut(s) 1152, 1287, 1557, 2021, 2078
BseXI GCAGC 5 cut(s) 628, 1150, 1931, 2039, 2233
BshFI GGCC 5 cut(s) 39, 492, 1309, 1695, 1773
BshNI GGYRCC 1 cut(s) 546
BsiHKAI GWGCWC 1 cut(s) 1133
BsiHKCI CYCGRG 2 cut(s) 401, 2390
BslFI GGGAC 2 cut(s) 938, 1797
BslI CCNNNNNNNGG 3 cut(s) 61, 1780, 1781
BsmAI GTCTC 6 cut(s) 360, 574, 1070, 1985, 2039, 2387
BsmBI CGTCTC 2 cut(s) 574, 2387
BsmFI GGGAC 2 cut(s) 938, 1797
BsmI GAATGC 2 cut(s) 690, 1936
BsnI GGCC 5 cut(s) 39, 492, 1309, 1695, 1773
Bso31I GGTCTC 2 cut(s) 1985, 2039
BsoBI CYCGRG 2 cut(s) 401, 2390
Bsp1286I GDGCHC 2 cut(s) 1133, 2326
Bsp1407I TGTACA 2 cut(s) 1544, 1757
Bsp1720I GCTNAGC 1 cut(s) 2066
Bsp19I CCATGG 1 cut(s) 1296
BspACI CCGC 2 cut(s) 544, 2077
BspANI GGCC 5 cut(s) 39, 492, 1309, 1695, 1773
BspCNI CTCAG 5 cut(s) 481, 921, 1413, 1720, 2449
BspHI TCATGA 2 cut(s) 187, 1068
BspLI GGNNCC 6 cut(s) 408, 548, 874, 1324, 1774, 2187
BspMAI CTGCAG 1 cut(s) 1061
BspMI ACCTGC 1 cut(s) 2021
BspPI GGATC 2 cut(s) 73, 1903
BspT107I GGYRCC 1 cut(s) 546
BspTI CTTAAG 2 cut(s) 1327, 2003
BspTNI GGTCTC 2 cut(s) 1985, 2039
BsrGI TGTACA 2 cut(s) 1544, 1757
BsrI ACTGG 5 cut(s) 1152, 1287, 1557, 2021, 2078
BssECI CCNNGG 7 cut(s) 402, 409, 1015, 1296, 1511, 1780, 1912
BssNI GRCGYC 2 cut(s) 547, 586
BssT1I CCWWGG 2 cut(s) 1015, 1296
Bst2UI CCWGG 4 cut(s) 411, 877, 1362, 1781
Bst4CI ACNGT 4 cut(s) 32, 256, 1445, 1656
Bst6I CTCTTC 3 cut(s) 625, 1110, 1503
BstACI GRCGYC 2 cut(s) 547, 586
BstAFI CTTAAG 2 cut(s) 1327, 2003
BstAUI TGTACA 2 cut(s) 1544, 1757
BstBAI YACGTR 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 773, 1533
BstDSI CCRYGG 2 cut(s) 1296, 1511
BstF5I GGATG 1 cut(s) 2431
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 2 cut(s) 549, 1484
BstMAI GTCTC 6 cut(s) 360, 574, 1070, 1985, 2039, 2387
BstNI CCWGG 4 cut(s) 411, 877, 1362, 1781
BstNSI RCATGY 6 cut(s) 863, 1535, 1582, 1758, 2059, 2120
BstSCI CCNGG 4 cut(s) 409, 875, 1360, 1779
BstSFI CTRYAG 2 cut(s) 18, 1057
BstV1I GCAGC 5 cut(s) 628, 1150, 1931, 2039, 2233
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 5 cut(s) 14, 65, 91, 1100, 1908
BstYI RGATCY 5 cut(s) 14, 65, 91, 1100, 1908
Bsu36I CCTNAGG 1 cut(s) 2436
BsuI GTATCC 2 cut(s) 2174, 2392
BsuRI GGCC 5 cut(s) 39, 492, 1309, 1695, 1773
BtgI CCRYGG 2 cut(s) 1296, 1511
BtsCI GGATG 1 cut(s) 2431
BtsIMutI CAGTG 4 cut(s) 28, 733, 1550, 2071
BveI ACCTGC 1 cut(s) 2021
Cac8I GCNNGC 2 cut(s) 773, 1533
CciI TCATGA 2 cut(s) 187, 1068
CfoI GCGC 2 cut(s) 549, 1484
Cfr13I GGNCC 6 cut(s) 406, 490, 1693, 1772, 2010, 2185
CseI GACGC 1 cut(s) 2262
CsiI ACCWGGT 1 cut(s) 875
Csp6I GTAC 9 cut(s) 23, 257, 528, 1155, 1545, 1725, 1758, 2114, 2176
CspCI CAANNNNNGTGG 2 cut(s) 636, 671
CviQI GTAC 9 cut(s) 23, 257, 528, 1155, 1545, 1725, 1758, 2114, 2176
DinI GGCGCC 1 cut(s) 548
DraI TTTAAA 2 cut(s) 270, 789
DriI GACNNNNNGTC 1 cut(s) 1998
EaeI YGGCCR 1 cut(s) 1307
Eam1104I CTCTTC 3 cut(s) 625, 1110, 1503
Eam1105I GACNNNNNGTC 1 cut(s) 1998
EarI CTCTTC 3 cut(s) 625, 1110, 1503
Eco130I CCWWGG 2 cut(s) 1015, 1296
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 2326
Eco31I GGTCTC 2 cut(s) 1985, 2039
Eco32I GATATC 1 cut(s) 609
Eco47I GGWCC 3 cut(s) 406, 2010, 2185
Eco57I CTGAAG 1 cut(s) 1091
Eco72I CACGTG 1 cut(s) 124
Eco81I CCTNAGG 1 cut(s) 2436
Eco88I CYCGRG 2 cut(s) 401, 2390
EcoO109I RGGNCCY 3 cut(s) 406, 490, 2185
EcoRI GAATTC 1 cut(s) 291
EcoRII CCWGG 4 cut(s) 409, 875, 1360, 1779
EcoRV GATATC 1 cut(s) 609
EcoT14I CCWWGG 2 cut(s) 1015, 1296
EcoT22I ATGCAT 1 cut(s) 1938
EcoT38I GRGCYC 1 cut(s) 2326
EgeI GGCGCC 1 cut(s) 548
EheI GGCGCC 1 cut(s) 548
ErhI CCWWGG 2 cut(s) 1015, 1296
Esp3I CGTCTC 2 cut(s) 574, 2387
FaqI GGGAC 2 cut(s) 938, 1797
FbaI TGATCA 3 cut(s) 184, 1680, 2419
FblI GTMKAC 2 cut(s) 589, 1570
Fnu4HI GCNGC 6 cut(s) 545, 642, 1164, 1945, 2028, 2247
FokI GGATG 1 cut(s) 2418
FriOI GRGCYC 1 cut(s) 2326
Fsp4HI GCNGC 6 cut(s) 545, 642, 1164, 1945, 2028, 2247
FspBI CTAG 9 cut(s) 47, 260, 302, 317, 824, 887, 1025, 1188, 2349
FspI TGCGCA 1 cut(s) 1483
GlaI GCGC 2 cut(s) 548, 1483
GluI GCNGC 6 cut(s) 545, 642, 1164, 1945, 2028, 2247
GsuI CTGGAG 2 cut(s) 1442, 1763
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 5 cut(s) 39, 492, 1309, 1695, 1773
HgaI GACGC 1 cut(s) 2262
HhaI GCGC 2 cut(s) 549, 1484
Hin1I GRCGYC 2 cut(s) 547, 586
Hin6I GCGC 2 cut(s) 547, 1482
HinP1I GCGC 2 cut(s) 547, 1482
HincII GTYRAC 2 cut(s) 590, 1669
HindII GTYRAC 2 cut(s) 590, 1669
HindIII AAGCTT 5 cut(s) 163, 1073, 1136, 1274, 1636
HphI GGTGA 2 cut(s) 390, 1376
Hpy166II GTNNAC 7 cut(s) 590, 658, 1571, 1669, 1845, 2010, 2176
Hpy188I TCNGA 6 cut(s) 448, 1219, 1354, 1422, 1913, 2461
Hpy8I GTNNAC 7 cut(s) 590, 658, 1571, 1669, 1845, 2010, 2176
Hpy99I CGWCG 2 cut(s) 588, 591
HpyCH4III ACNGT 4 cut(s) 32, 256, 1445, 1656
HpyCH4IV ACGT 3 cut(s) 123, 450, 586
HpySE526I ACGT 3 cut(s) 123, 450, 586
Hsp92I GRCGYC 2 cut(s) 547, 586
HspAI GCGC 2 cut(s) 547, 1482
KasI GGCGCC 1 cut(s) 546
Ksp22I TGATCA 3 cut(s) 184, 1680, 2419
LmnI GCTCC 4 cut(s) 708, 1461, 1512, 1854
Lsp1109I GCAGC 5 cut(s) 628, 1150, 1931, 2039, 2233
LweI GCATC 2 cut(s) 653, 2276
MabI ACCWGGT 1 cut(s) 875
MaeI CTAG 9 cut(s) 47, 260, 302, 317, 824, 887, 1025, 1188, 2349
MaeII ACGT 3 cut(s) 123, 450, 586
MaeIII GTNAC 4 cut(s) 533, 596, 928, 2312
MflI RGATCY 5 cut(s) 14, 65, 91, 1100, 1908
MhlI GDGCHC 2 cut(s) 1133, 2326
MlsI TGGCCA 1 cut(s) 1309
MluNI TGGCCA 1 cut(s) 1309
Mly113I GGCGCC 1 cut(s) 547
MlyI GAGTC 5 cut(s) 960, 1456, 1525, 1576, 1779
MmeI TCCRAC 3 cut(s) 1103, 2233, 2239
Mox20I TGGCCA 1 cut(s) 1309
Mph1103I ATGCAT 1 cut(s) 1938
MroXI GAANNNNTTC 4 cut(s) 39, 200, 574, 744
MscI TGGCCA 1 cut(s) 1309
MslI CAYNNNNRTG 2 cut(s) 1243, 1676
Msp20I TGGCCA 1 cut(s) 1309
MspCI CTTAAG 2 cut(s) 1327, 2003
MspR9I CCNGG 4 cut(s) 411, 877, 1362, 1781
Mva1269I GAATGC 2 cut(s) 690, 1936
MvaI CCWGG 4 cut(s) 411, 877, 1362, 1781
NarI GGCGCC 1 cut(s) 547
NcoI CCATGG 1 cut(s) 1296
NlaIV GGNNCC 6 cut(s) 408, 548, 874, 1324, 1774, 2187
NmuCI GTSAC 1 cut(s) 928
NsbI TGCGCA 1 cut(s) 1483
NsiI ATGCAT 1 cut(s) 1938
NspI RCATGY 6 cut(s) 863, 1535, 1582, 1758, 2059, 2120
PaeI GCATGC 1 cut(s) 1535
PagI TCATGA 2 cut(s) 187, 1068
PceI AGGCCT 1 cut(s) 39
PciI ACATGT 4 cut(s) 859, 1578, 1754, 2116
PctI GAATGC 2 cut(s) 690, 1936
PdmI GAANNNNTTC 4 cut(s) 39, 200, 574, 744
PflFI GACNNNGTC 2 cut(s) 585, 1240
PflMI CCANNNNNTGG 1 cut(s) 61
PkrI GCNGC 6 cut(s) 546, 643, 1165, 1946, 2029, 2248
PleI GAGTC 5 cut(s) 959, 1456, 1524, 1575, 1779
PluTI GGCGCC 1 cut(s) 550
PmaCI CACGTG 1 cut(s) 124
PmlI CACGTG 1 cut(s) 124
PpsI GAGTC 5 cut(s) 959, 1456, 1524, 1575, 1779
Ppu21I YACGTR 1 cut(s) 124
PpuMI RGGWCCY 2 cut(s) 406, 2185
PscI ACATGT 4 cut(s) 859, 1578, 1754, 2116
PshBI ATTAAT 1 cut(s) 758
Psp5II RGGWCCY 2 cut(s) 406, 2185
Psp6I CCWGG 4 cut(s) 409, 875, 1360, 1779
PspCI CACGTG 1 cut(s) 124
PspGI CCWGG 4 cut(s) 409, 875, 1360, 1779
PspN4I GGNNCC 6 cut(s) 408, 548, 874, 1324, 1774, 2187
PspPI GGNCC 6 cut(s) 406, 490, 1693, 1772, 2010, 2185
PspPPI RGGWCCY 2 cut(s) 406, 2185
PstI CTGCAG 1 cut(s) 1061
PsuI RGATCY 5 cut(s) 14, 65, 91, 1100, 1908
PsyI GACNNNGTC 2 cut(s) 585, 1240
RsaI GTAC 9 cut(s) 24, 258, 529, 1156, 1546, 1726, 1759, 2115, 2177
RsaNI GTAC 9 cut(s) 23, 257, 528, 1155, 1545, 1725, 1758, 2114, 2176
RseI CAYNNNNRTG 2 cut(s) 1243, 1676
SalI GTCGAC 1 cut(s) 588
SatI GCNGC 6 cut(s) 545, 642, 1164, 1945, 2028, 2247
Sau96I GGNCC 6 cut(s) 406, 490, 1693, 1772, 2010, 2185
ScaI AGTACT 1 cut(s) 258
SchI GAGTC 5 cut(s) 960, 1456, 1525, 1576, 1779
ScrFI CCNGG 4 cut(s) 411, 877, 1362, 1781
SduI GDGCHC 2 cut(s) 1133, 2326
SexAI ACCWGGT 1 cut(s) 875
SfaNI GCATC 2 cut(s) 653, 2276
SfcI CTRYAG 2 cut(s) 18, 1057
SfoI GGCGCC 1 cut(s) 548
SinI GGWCC 3 cut(s) 406, 2010, 2185
SmiI ATTTAAAT 1 cut(s) 270
SmiMI CAYNNNNRTG 2 cut(s) 1243, 1676
SmlI CTYRAG 5 cut(s) 962, 1327, 1466, 1904, 2003
SmoI CTYRAG 5 cut(s) 962, 1327, 1466, 1904, 2003
SphI GCATGC 1 cut(s) 1535
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 2 cut(s) 544, 2077
SspDI GGCGCC 1 cut(s) 546
SspI AATATT 1 cut(s) 522
SspMI CTAG 9 cut(s) 47, 260, 302, 317, 824, 887, 1025, 1188, 2349
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 4 cut(s) 409, 875, 1360, 1779
StyI CCWWGG 2 cut(s) 1015, 1296
SwaI ATTTAAAT 1 cut(s) 270
TaaI ACNGT 4 cut(s) 32, 256, 1445, 1656
TaiI ACGT 3 cut(s) 126, 453, 589
TaqI TCGA 6 cut(s) 578, 589, 711, 1235, 1644, 2234
TatI WGTACW 6 cut(s) 22, 256, 527, 1544, 1757, 2113
TauI GCSGC 1 cut(s) 547
TscAI CASTG 4 cut(s) 35, 733, 1557, 2078
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 5 cut(s) 641, 1163, 1944, 2027, 2246
Tsp45I GTSAC 1 cut(s) 928
TspGWI ACGGA 2 cut(s) 1528, 2411
TspRI CASTG 4 cut(s) 35, 733, 1557, 2078
Tth111I GACNNNGTC 2 cut(s) 585, 1240
Van91I CCANNNNNTGG 1 cut(s) 61
Vha464I CTTAAG 2 cut(s) 1327, 2003
VpaK11BI GGWCC 3 cut(s) 406, 2010, 2185
VspI ATTAAT 1 cut(s) 758
XapI RAATTY 8 cut(s) 196, 271, 291, 631, 806, 897, 991, 2135
XceI RCATGY 6 cut(s) 863, 1535, 1582, 1758, 2059, 2120
XcmI CCANNNNNNNNNTGG 1 cut(s) 1294
XmiI GTMKAC 2 cut(s) 589, 1570
XmnI GAANNNNTTC 4 cut(s) 39, 200, 574, 744
XspI CTAG 9 cut(s) 47, 260, 302, 317, 824, 887, 1025, 1188, 2349
ZraI GACGTC 1 cut(s) 587
ZrmI AGTACT 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 1938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.