MD17G1275900.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000654
Physical Location & Seq
Forward (+)
33675273 .. 33679564
4292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1275900.v1.1.491

Sequence Viewer

Length: 3861 bp
ATGGATGCTTTCCGAGATTCCTCTTCCTCCTCTTACCGGTGTTCCTATCATGCCTTCTTGAGTTTCAGAGGCCAGGACACGCGCAGGGGCTTTACTGATCACCTCTATAGAGCCTTGGAGCTAGCAGGAATCCACACGTTTAGAGACGATGATGAAATCAAGAGAGGGGAGAATATTGAGTCGGAGTTAGACAAGGCGATACAGGAGTCACAAGTATCGATAATTGTCTTCTCCAAGGACTACGCTTCCTCAAGGTGGTGTTTGAACGAACTTCTGAAGATCGTGGAACGTAGAAATACTGATCATAGACATGTGGTTCTGCCAGTTTTCTATGATGTGGATCCATCCGATGTCAGGAAGCAGAGTGGTACTTTTGCTAAAGCATTTGCCAGACATGAGGAGCGATTCAGTACCGAGATGGACAAGGTGGAGCAGTGGAGAAGAGCTCTTGGAGATGTTGCATCTTTGGGAGGCATGGTTTTAGGAGATCGGTATGAGGGGCAGTTCATCCAAGATGTTGTTGAAGAGATTAGTAATAAACTGGATCCCGCAGCTTTAGACGTTGCTCCCTTTGCAGTTGGAATAGATAATCGTGTGCAAGACCTAAATATGTGGTTACAAGATGGATCAAATGATGTTGGTGTAGCTGTCATCTGTGGTATGGGTGGAATTGGCAAGAGCACCATTGTGAAAGCTGCTTATAACCAAAACTTTGATAGATTTCAAGGTAGCAGCTTTCTTGCAGATATACGAGAATCTTCAGAACAACCTAATGGTTTTGCTCGCTTGCAAAGAAAACTTCTTTTAGATATCCAAAAGGGGAACGCAAAGAAAGTATACAATGTGGCTGAAGGAATAAGCAGGATCAAACGAGCCGTAGGCTATAAAAGAGTTCTTATTGTTCTCGATGATGTGAACAGCCGGGATCAACTCAATGCAATTCTTGGAATGCGAGAATGGCTTCATCCAGGAAGTAAAATTATCATAACAACTCGACATGAACATTTGTTAAATGCTCATGAAGTTTGTGAAAAGTTTATGGTTCGAGAACTGGATGAAGATGAATCACTTGAGCTTTTCAGTTGGCATGCCTTCGGACAATCCCATCCGGTAGAAGGTTACATGGAGCTTTCAAGACATGTGGTGCAGCATTGTGGAGGGCTTCCATTAGCTATTCAAGTTCTGGGATCTTCTTTATCTGGAAAAAGTGTAGAAGTATGGCAAAGTGCATTGCAGAAGTTAGACGTGATCCCTAATGACAAAATTCAAAAAGTTCTGAGGATAAGTTTTGATTCTTTACACGAAGATCATGACCAAAATTTATTCCTTCATATAGCTAGCTTCTTCACAGGAAAGACGATGGATTATATAATTACAATACTGGACAACTTAGAGTTTTGCACAAGGGTTGGGATTGAAAATCTAGTTGATAGATGTCTAGTGACAATTCAAGGGACGAAGTTGGTCATGCATCAATTGTTGAGAAACATGGCAATGGCAATTATTCGCAAAGAATCACCTCATGACCCTGGAAAACGTAGTAGAGTGTTGCAGAACGATGCCTCTAATATTTTGAGAAAATTAAGTGGCACAGAAAATATTAAGGGCCTCATGCTCAACCTTGCTAGCAAAAGAACATTTGTTGGAAGTGATAACAAACGATGCCATGCTGAAGATTATGATGGAAACTGTTCAAGACGACGTCGGCTTGGTTTCTTCTCTTGGAAGCCAGTTAGTTTTTCCTCCACAAACTCAGCTTCTGCATCAAATGAGATAGATTTCAAATCTGAGGCTTTTAGAAGGATGCACAATCTTGAAATTCTCATGCTCAACAACGTCAACGTCAGGGGAAGCTACGAAGAATTTTCAAAAAAATTAGTATGTTTATCTTGGCGAGGATTCCACTTAAAATCAATACCCGAAAATTTTTGTTTGGAAAACCTGGTTGTTCTAGACATGCGAAATAGTAGCCTACAACATGTTTGGAATGAGACCAGGGTTATTCCGAGGCTGAAGATTCTTAATTTCAGTCATTCGCATGGCCTTAGGACAGTCCCCGATTTCTCAGGACTCTATAACCTTGAGAGGTTGATTCTTAAACATTGCATAAATTTGGTTGAGGTTCATGAGTCCATCGGAAACCTAGAAAAACTTGCTGTTTTGAATCTAGAAGGCTGCAAAAATCTCATGAAGCTTCCAGTATTGAGATCTATTCAGGATCTTATTCTTTCTGGTTGCTCAAAGCTTGAAATGAAGAAACTCAATCTGGTATCAGCTAAATCATGGTATTCAGTCTGGTCGTGGGTGTCGCCAAGAAAACATACTGAATTAAGCAGTTTCTCATTGGCAAGTTTACCACGTTCTTTGATAAGTTTAAGTCTGGATTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTCGTTAGAGTACTTGAAGCTGGATGATAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGAGTCTTAAAGTATCTGGTTGCAGAAACCTCACAATGCTTCCAGAGCTCCCACGTTCTTTGACAAGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTTATTAGAGTACTTGGATCTTGATGGTAATCCGATTACAAGCCTACCAGAAAGCATGAACAATGTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCACGTTCTTTGACATGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCTAGTGCCCTAACTATGCTGTCCTCATTAGAGTACTTGGATCTTGATGGTAATCCGATTACAAGCCTACCAGAAAGCATGAACAATGTTGTTAAGCTCCAAACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCACGTTCTTTGACAGGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCAGTCCTCATTAGAGTACTTGGATCTTGATGGTAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCACGTTCTTTGACAGGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCAGTCCTCATTAGAGTACTTGGATCTTGATGGTAATCGGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCACGTTCTTTGACATGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCAGCCCTCATTAGAGTACTTGGATCTTGATTGTAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCAAGTTCTTTGACATGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTCGTTGGAGTACTTGGATCTTGATGGTAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCACGTTCTTTGACATGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCCTCGTTGGAGTACTTGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1287

Amino Acids

143.44

Weight (kDa)

6.26

Isoelectric Point (pI)

52.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 183 8.4e-51 TIR domain
TIR_2 PF13676 19 - 116 5.2e-17 TIR domain
NB-ARC PF00931 197 - 366 6.8e-20 NB-ARC domain
WHD_ROQ1 PF23282 436 - 506 2.3e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_8 PF13855 788 - 844 2.3e-07 Leucine rich repeat
LRR_14 PF23598 792 - 859 3.1e-06 Leucine-rich repeat region
LRR_8 PF13855 854 - 911 1.1e-06 Leucine rich repeat
LRR_14 PF23598 858 - 926 9e-06 Leucine-rich repeat region
LRR_8 PF13855 921 - 978 2.2e-06 Leucine rich repeat
LRR_14 PF23598 923 - 992 6.5e-06 Leucine-rich repeat region
LRR_8 PF13855 988 - 1045 1.7e-06 Leucine rich repeat
LRR_8 PF13855 1055 - 1112 2.3e-07 Leucine rich repeat
LRR_8 PF13855 1122 - 1179 9.1e-07 Leucine rich repeat
LRR_14 PF23598 1146 - 1215 4e-06 Leucine-rich repeat region
LRR_8 PF13855 1190 - 1246 2.8e-07 Leucine rich repeat
LRR_14 PF23598 1191 - 1262 3.2e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AatII GACGTC 1 cut(s) 1705
AccI GTMKAC 1 cut(s) 837
AccII CGCG 1 cut(s) 82
AciI CCGC 1 cut(s) 549
AcsI RAATTY 6 cut(s) 1263, 1318, 1818, 1862, 1924, 2110
AcuI CTGAAG 5 cut(s) 296, 744, 870, 1692, 2033
AcyI GRCGYC 1 cut(s) 1702
AfiI CCNNNNNNNGG 4 cut(s) 36, 255, 354, 1115
AflIII ACRYGT 8 cut(s) 135, 310, 1138, 1978, 2771, 3374, 3575, 3776
AgeI ACCGGT 1 cut(s) 36
AjiI CACGTC 1 cut(s) 1246
AjnI CCWGG 5 cut(s) 72, 967, 1528, 1941, 1994
AleI CACNNNNGTG 1 cut(s) 686
Alw21I GWGCWC 9 cut(s) 448, 683, 2556, 2757, 2958, 3159, 3360, 3561, 3762
Alw26I GTCTC 2 cut(s) 138, 1985
AlwNI CAGNNNCTG 1 cut(s) 1760
AoxI GGCC 3 cut(s) 70, 1606, 2041
ApoI RAATTY 6 cut(s) 1263, 1318, 1818, 1862, 1924, 2110
AsiGI ACCGGT 1 cut(s) 36
Asp700I GAANNNNTTC 2 cut(s) 960, 1690
AspLEI GCGC 1 cut(s) 84
AspS9I GGNCC 1 cut(s) 1606
AsuC2I CCSGG 1 cut(s) 923
AsuHPI GGTGA 2 cut(s) 92, 1509
AsuNHI GCTAGC 3 cut(s) 121, 1337, 1625
AxyI CCTNAGG 1 cut(s) 2045
BaeGI GKGCMC 8 cut(s) 2416, 2617, 2818, 3019, 3220, 3421, 3622, 3823
BaeI ACNNNNGTAYC 2 cut(s) 191, 224
BamHI GGATCC 2 cut(s) 340, 544
BanII GRGCYC 8 cut(s) 448, 2556, 2757, 2958, 3159, 3360, 3561, 3762
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 9 cut(s) 448, 683, 2556, 2757, 2958, 3159, 3360, 3561, 3762
BceAI ACGGC 1 cut(s) 860
BciT130I CCWGG 5 cut(s) 74, 969, 1530, 1943, 1996
BclI TGATCA 2 cut(s) 97, 301
BcnI CCSGG 1 cut(s) 923
BcoDI GTCTC 2 cut(s) 138, 1985
BfaI CTAG 9 cut(s) 122, 1338, 1424, 1439, 1626, 1952, 2144, 2168, 2811
BfmI CTRYAG 1 cut(s) 106
BglII AGATCT 1 cut(s) 2207
BmcAI AGTACT 8 cut(s) 2441, 2642, 2843, 3044, 3245, 3446, 3647, 3849
Bme1390I CCNGG 6 cut(s) 74, 923, 969, 1530, 1943, 1996
BmgBI CACGTC 1 cut(s) 1246
BmgT120I GGNCC 1 cut(s) 1606
BmiI GGNNCC 2 cut(s) 342, 546
BmrFI CCNGG 6 cut(s) 74, 923, 969, 1530, 1943, 1996
BmsI GCATC 6 cut(s) 470, 1480, 1549, 1652, 1772, 1794
BmtI GCTAGC 3 cut(s) 125, 1341, 1629
BpiI GAAGAC 1 cut(s) 220
BplI GAGNNNNNCTC 2 cut(s) 430, 462
BpmI CTGGAG 6 cut(s) 2489, 2690, 3092, 3293, 3494, 3695
BpuEI CTTGAG 4 cut(s) 79, 235, 1091, 2102
BpuMI CCSGG 1 cut(s) 923
Bsa29I ATCGAT 1 cut(s) 218
BsaBI GATNNNNATC 8 cut(s) 339, 2457, 2658, 2859, 3060, 3261, 3462, 3663
BsaHI GRCGYC 1 cut(s) 1702
BsaI GGTCTC 1 cut(s) 1985
BsaJI CCNNGG 5 cut(s) 114, 234, 1528, 1995, 2006
BsaWI WCCGGW 2 cut(s) 36, 1108
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 255, 354, 1115
Bse118I RCCGGY 1 cut(s) 36
Bse21I CCTNAGG 1 cut(s) 2045
Bse3DI GCAATG 3 cut(s) 1229, 1500, 2101
Bse8I GATNNNNATC 8 cut(s) 339, 2457, 2658, 2859, 3060, 3261, 3462, 3663
BseBI CCWGG 5 cut(s) 74, 969, 1530, 1943, 1996
BseCI ATCGAT 1 cut(s) 218
BseDI CCNNGG 5 cut(s) 114, 234, 1528, 1995, 2006
BseGI GGATG 8 cut(s) 10, 344, 507, 964, 1060, 1105, 1809, 2458
BseJI GATNNNNATC 8 cut(s) 339, 2457, 2658, 2859, 3060, 3261, 3462, 3663
BseLI CCNNNNNNNGG 4 cut(s) 36, 255, 354, 1115
BseMI GCAATG 3 cut(s) 1229, 1500, 2101
BseMII CTCAG 4 cut(s) 1268, 1767, 1779, 2079
BseRI GAGGAG 2 cut(s) 19, 413
BseSI GKGCMC 8 cut(s) 2416, 2617, 2818, 3019, 3220, 3421, 3622, 3823
BsgI GTGCAG 1 cut(s) 1166
Bsh1236I CGCG 1 cut(s) 82
BshFI GGCC 3 cut(s) 72, 1608, 2043
BshTI ACCGGT 1 cut(s) 36
BshVI ATCGAT 1 cut(s) 218
BsiHKAI GWGCWC 9 cut(s) 448, 683, 2556, 2757, 2958, 3159, 3360, 3561, 3762
BsiSI CCGG 3 cut(s) 37, 922, 1109
BslFI GGGAC 3 cut(s) 1468, 2039, 3820
BslI CCNNNNNNNGG 4 cut(s) 36, 255, 354, 1115
BsmAI GTCTC 2 cut(s) 138, 1985
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 3 cut(s) 1468, 2039, 3820
BsmI GAATGC 1 cut(s) 954
BsnI GGCC 3 cut(s) 72, 1608, 2043
Bso31I GGTCTC 1 cut(s) 1985
BspACI CCGC 1 cut(s) 549
BspANI GGCC 3 cut(s) 72, 1608, 2043
BspCNI CTCAG 4 cut(s) 1269, 1766, 1780, 2078
BspDI ATCGAT 1 cut(s) 218
BspFNI CGCG 1 cut(s) 82
BspHI TCATGA 5 cut(s) 1018, 1309, 1522, 2125, 2187
BspLI GGNNCC 2 cut(s) 342, 546
BspOI GCTAGC 3 cut(s) 125, 1341, 1629
BspQI GCTCTTC 1 cut(s) 436
BspTNI GGTCTC 1 cut(s) 1985
BsrDI GCAATG 3 cut(s) 1229, 1500, 2101
BsrFI RCCGGY 1 cut(s) 36
BssAI RCCGGY 1 cut(s) 36
BssECI CCNNGG 5 cut(s) 114, 234, 1528, 1995, 2006
BssNAI GTATAC 1 cut(s) 838
BssNI GRCGYC 1 cut(s) 1702
BssT1I CCWWGG 2 cut(s) 114, 234
Bst1107I GTATAC 1 cut(s) 838
Bst2UI CCWGG 5 cut(s) 74, 969, 1530, 1943, 1996
Bst4CI ACNGT 2 cut(s) 1691, 2053
Bst6I CTCTTC 3 cut(s) 28, 436, 519
BstACI GRCGYC 1 cut(s) 1702
BstDEI CTNAG 6 cut(s) 1277, 1390, 1753, 1788, 2045, 2065
BstF5I GGATG 8 cut(s) 10, 344, 507, 964, 1060, 1105, 1809, 2458
BstFNI CGCG 1 cut(s) 82
BstHHI GCGC 1 cut(s) 84
BstMAI GTCTC 2 cut(s) 138, 1985
BstMWI GCNNNNNNNGC 3 cut(s) 572, 958, 2551
BstNI CCWGG 5 cut(s) 74, 969, 1530, 1943, 1996
BstNSI RCATGY 9 cut(s) 314, 1091, 1142, 1960, 1982, 2775, 3378, 3579, 3780
BstSCI CCNGG 6 cut(s) 72, 921, 967, 1528, 1941, 1994
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 8 cut(s) 2416, 2617, 2818, 3019, 3220, 3421, 3622, 3823
BstUI CGCG 1 cut(s) 82
BstV2I GAAGAC 1 cut(s) 220
BstZ17I GTATAC 1 cut(s) 838
Bsu15I ATCGAT 1 cut(s) 218
Bsu36I CCTNAGG 1 cut(s) 2045
BsuRI GGCC 3 cut(s) 72, 1608, 2043
BsuTUI ATCGAT 1 cut(s) 218
BtrI CACGTC 1 cut(s) 1246
BtsCI GGATG 8 cut(s) 10, 344, 507, 964, 1060, 1105, 1809, 2458
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 8 cut(s) 440, 2579, 2780, 2981, 3182, 3383, 3584, 3785
CaiI CAGNNNCTG 1 cut(s) 1760
CciI TCATGA 5 cut(s) 1018, 1309, 1522, 2125, 2187
CfoI GCGC 1 cut(s) 84
Cfr10I RCCGGY 1 cut(s) 36
Cfr13I GGNCC 1 cut(s) 1606
ClaI ATCGAT 1 cut(s) 218
CsiI ACCWGGT 1 cut(s) 1941
CspAI ACCGGT 1 cut(s) 36
DdeI CTNAG 6 cut(s) 1277, 1390, 1753, 1788, 2045, 2065
Eam1104I CTCTTC 3 cut(s) 28, 436, 519
EarI CTCTTC 3 cut(s) 28, 436, 519
Ecl136II GAGCTC 8 cut(s) 446, 2554, 2755, 2956, 3157, 3358, 3559, 3760
Eco130I CCWWGG 2 cut(s) 114, 234
Eco24I GRGCYC 8 cut(s) 448, 2556, 2757, 2958, 3159, 3360, 3561, 3762
Eco31I GGTCTC 1 cut(s) 1985
Eco32I GATATC 1 cut(s) 811
Eco53kI GAGCTC 8 cut(s) 446, 2554, 2755, 2956, 3157, 3358, 3559, 3760
Eco57I CTGAAG 5 cut(s) 296, 744, 870, 1692, 2033
Eco81I CCTNAGG 1 cut(s) 2045
EcoICRI GAGCTC 8 cut(s) 446, 2554, 2755, 2956, 3157, 3358, 3559, 3760
EcoO109I RGGNCCY 1 cut(s) 1606
EcoRII CCWGG 5 cut(s) 72, 967, 1528, 1941, 1994
EcoRV GATATC 1 cut(s) 811
EcoT14I CCWWGG 2 cut(s) 114, 234
EcoT22I ATGCAT 1 cut(s) 1473
EcoT38I GRGCYC 8 cut(s) 448, 2556, 2757, 2958, 3159, 3360, 3561, 3762
ErhI CCWWGG 2 cut(s) 114, 234
Esp3I CGTCTC 1 cut(s) 138
FaqI GGGAC 3 cut(s) 1468, 2039, 3820
FauI CCCGC 1 cut(s) 556
FbaI TGATCA 2 cut(s) 97, 301
FblI GTMKAC 1 cut(s) 837
FokI GGATG 8 cut(s) 17, 331, 494, 951, 1067, 1092, 1816, 2465
FriOI GRGCYC 8 cut(s) 448, 2556, 2757, 2958, 3159, 3360, 3561, 3762
FspBI CTAG 9 cut(s) 122, 1338, 1424, 1439, 1626, 1952, 2144, 2168, 2811
GlaI GCGC 1 cut(s) 83
GsuI CTGGAG 6 cut(s) 2489, 2690, 3092, 3293, 3494, 3695
HaeIII GGCC 3 cut(s) 72, 1608, 2043
HapII CCGG 3 cut(s) 37, 922, 1109
HhaI GCGC 1 cut(s) 84
Hin1I GRCGYC 1 cut(s) 1702
Hin6I GCGC 1 cut(s) 82
HinP1I GCGC 1 cut(s) 82
HincII GTYRAC 1 cut(s) 1840
HindII GTYRAC 1 cut(s) 1840
HindIII AAGCTT 2 cut(s) 2192, 2243
HpaII CCGG 3 cut(s) 37, 922, 1109
HphI GGTGA 2 cut(s) 92, 1509
Hpy166II GTNNAC 4 cut(s) 838, 916, 1840, 2354
Hpy8I GTNNAC 4 cut(s) 838, 916, 1840, 2354
Hpy99I CGWCG 2 cut(s) 1704, 1707
HpyAV CCTTC 7 cut(s) 64, 845, 1102, 1109, 1337, 1794, 2165
HpyCH4III ACNGT 2 cut(s) 1691, 2053
HpyF10VI GCNNNNNNNGC 3 cut(s) 572, 958, 2551
HpyF3I CTNAG 6 cut(s) 1277, 1390, 1753, 1788, 2045, 2065
Hsp92I GRCGYC 1 cut(s) 1702
HspAI GCGC 1 cut(s) 82
Ksp22I TGATCA 2 cut(s) 97, 301
LguI GCTCTTC 1 cut(s) 436
LweI GCATC 6 cut(s) 470, 1480, 1549, 1652, 1772, 1794
MabI ACCWGGT 1 cut(s) 1941
MaeI CTAG 9 cut(s) 122, 1338, 1424, 1439, 1626, 1952, 2144, 2168, 2811
MaeIII GTNAC 4 cut(s) 207, 615, 1118, 1441
MfeI CAATTG 1 cut(s) 1475
MmeI TCCRAC 5 cut(s) 162, 559, 1624, 3621, 3823
Mph1103I ATGCAT 1 cut(s) 1473
MroXI GAANNNNTTC 2 cut(s) 960, 1690
MslI CAYNNNNRTG 4 cut(s) 309, 686, 1493, 2037
MspI CCGG 3 cut(s) 37, 922, 1109
MspR9I CCNGG 6 cut(s) 74, 923, 969, 1530, 1943, 1996
MunI CAATTG 1 cut(s) 1475
Mva1269I GAATGC 1 cut(s) 954
MvaI CCWGG 5 cut(s) 74, 969, 1530, 1943, 1996
MvnI CGCG 1 cut(s) 82
MwoI GCNNNNNNNGC 3 cut(s) 572, 958, 2551
NciI CCSGG 1 cut(s) 923
NheI GCTAGC 3 cut(s) 121, 1337, 1625
NlaIV GGNNCC 2 cut(s) 342, 546
NmuCI GTSAC 2 cut(s) 207, 1441
NsiI ATGCAT 1 cut(s) 1473
NspI RCATGY 9 cut(s) 314, 1091, 1142, 1960, 1982, 2775, 3378, 3579, 3780
OliI CACNNNNGTG 1 cut(s) 686
PaeI GCATGC 1 cut(s) 1091
PagI TCATGA 5 cut(s) 1018, 1309, 1522, 2125, 2187
PciI ACATGT 7 cut(s) 310, 1138, 1978, 2771, 3374, 3575, 3776
PciSI GCTCTTC 1 cut(s) 436
PctI GAATGC 1 cut(s) 954
PdmI GAANNNNTTC 2 cut(s) 960, 1690
PflFI GACNNNGTC 1 cut(s) 1701
PfoI TCCNGGA 1 cut(s) 967
PinAI ACCGGT 1 cut(s) 36
PscI ACATGT 7 cut(s) 310, 1138, 1978, 2771, 3374, 3575, 3776
PsiI TTATAA 1 cut(s) 702
Psp124BI GAGCTC 8 cut(s) 448, 2556, 2757, 2958, 3159, 3360, 3561, 3762
Psp6I CCWGG 5 cut(s) 72, 967, 1528, 1941, 1994
PspGI CCWGG 5 cut(s) 72, 967, 1528, 1941, 1994
PspN4I GGNNCC 2 cut(s) 342, 546
PspPI GGNCC 1 cut(s) 1606
PstNI CAGNNNCTG 1 cut(s) 1760
PsyI GACNNNGTC 1 cut(s) 1701
RseI CAYNNNNRTG 4 cut(s) 309, 686, 1493, 2037
SacI GAGCTC 8 cut(s) 448, 2556, 2757, 2958, 3159, 3360, 3561, 3762
SapI GCTCTTC 1 cut(s) 436
Sau96I GGNCC 1 cut(s) 1606
ScaI AGTACT 8 cut(s) 2441, 2642, 2843, 3044, 3245, 3446, 3647, 3849
ScrFI CCNGG 6 cut(s) 74, 923, 969, 1530, 1943, 1996
SexAI ACCWGGT 1 cut(s) 1941
SfaNI GCATC 6 cut(s) 470, 1480, 1549, 1652, 1772, 1794
SfcI CTRYAG 1 cut(s) 106
SmiMI CAYNNNNRTG 4 cut(s) 309, 686, 1493, 2037
SmlI CTYRAG 4 cut(s) 58, 250, 1070, 2081
SmoI CTYRAG 4 cut(s) 58, 250, 1070, 2081
SphI GCATGC 1 cut(s) 1091
SsiI CCGC 1 cut(s) 549
SspI AATATT 3 cut(s) 175, 1570, 1600
SspMI CTAG 9 cut(s) 122, 1338, 1424, 1439, 1626, 1952, 2144, 2168, 2811
SstI GAGCTC 8 cut(s) 448, 2556, 2757, 2958, 3159, 3360, 3561, 3762
StyD4I CCNGG 6 cut(s) 72, 921, 967, 1528, 1941, 1994
StyI CCWWGG 2 cut(s) 114, 234
TaaI ACNGT 2 cut(s) 1691, 2053
TaqI TCGA 4 cut(s) 218, 906, 994, 1045
TatI WGTACW 8 cut(s) 2439, 2640, 2841, 3042, 3243, 3444, 3645, 3847
TscAI CASTG 8 cut(s) 440, 2586, 2787, 2988, 3189, 3390, 3591, 3792
TseFI GTSAC 2 cut(s) 207, 1441
Tsp45I GTSAC 2 cut(s) 207, 1441
TspRI CASTG 8 cut(s) 440, 2586, 2787, 2988, 3189, 3390, 3591, 3792
Tth111I GACNNNGTC 1 cut(s) 1701
XapI RAATTY 6 cut(s) 1263, 1318, 1818, 1862, 1924, 2110
XbaI TCTAGA 2 cut(s) 1951, 2167
XceI RCATGY 9 cut(s) 314, 1091, 1142, 1960, 1982, 2775, 3378, 3579, 3780
XmiI GTMKAC 1 cut(s) 837
XmnI GAANNNNTTC 2 cut(s) 960, 1690
XspI CTAG 9 cut(s) 122, 1338, 1424, 1439, 1626, 1952, 2144, 2168, 2811
ZraI GACGTC 1 cut(s) 1703
ZrmI AGTACT 8 cut(s) 2441, 2642, 2843, 3044, 3245, 3446, 3647, 3849
Zsp2I ATGCAT 1 cut(s) 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.