Prupe.3G130800_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000654
Physical Location & Seq
Reverse (-)
13369597 .. 13370649
1053 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G130800.1

Sequence Viewer

Length: 894 bp
ATGTTTGAATCATTGGATTCAAGTCTATGGAAATGCAAGAAATTAGTTGAAGTTCAAAGCTTGTTTGATATAAAACCGTTGAAAAGGGTTGACATAGAAATGATCAGGGATATGGGCCTGTTTAATTTGGAATCCACAGGCGTAGGTACTGAGGTTGAAATGACCAACTACCTGACATGTACAACAAGGAACGGGCCCCTCCAGGGACTGTATGAATGTGGCATATTTAGTATTTTCGTTCATGGAAACAAGATTCCAGATCGGTTCACCTACAGGAGCATGGGTAAATCTGTTTTGTCTATCATTCTACCATCACATTTCAATCTCAAGATCCGAGGCTTAAATATATGTGTTATGTATTCACGCCGCCCATTTTGGTTTAGTAGTAGAAACTTTCTTAAAGTCAGTAATGAGACCAAAGGTCTTAAGTGGACCTATTGCCCATTTGCAGCAGGTCTCCCAAAAAAGAACCAAGACGTGCTATGGCTAAGCCACTGGCGGTTTGAAAATGATGAATTGGAGGAAGGAGAACAAGTTCATGTTTCGATAAATGAGGAATTTAGTTTCTGGGCCAAGAAGTTCTGTATCCAGCTTGTGTACGAGAAGGACCCATCAAATAGTGAAGATATAATAATCCAACAGGAAACACCTTCTTCAAGCCAGATTGCTGCTGGTGGAAATGTATCAGCATCAGCGTCTAAGTATCAATTCTGGACGGGCAAATACTTTCTATGTAACCATCGGGCTCGTATTCATCAACATCAATTCTCTAGGAGGCAGATGAACCCATCTTATCTTGAACATTACAAGCCCGAGACGGATACTTTTCATTACTTATTTGATCAAGATGTTCATCCTCAAGACAATCATTCCATCTATTCTGAAGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

298

Amino Acids

34.71

Weight (kDa)

6.6

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 443
AciI CCGC 2 cut(s) 367, 499
AclWI GGATC 1 cut(s) 325
AcsI RAATTY 1 cut(s) 557
AfaI GTAC 3 cut(s) 148, 181, 599
AfiI CCNNNNNNNGG 1 cut(s) 203
AflII CTTAAG 1 cut(s) 425
AflIII ACRYGT 1 cut(s) 176
AhdI GACNNNNNGTC 1 cut(s) 420
AjiI CACGTC 1 cut(s) 478
AjnI CCWGG 1 cut(s) 201
AloI GAACNNNNNNTCC 2 cut(s) 519, 551
AluBI AGCT 2 cut(s) 60, 592
AluI AGCT 2 cut(s) 60, 592
Alw26I GTCTC 3 cut(s) 407, 461, 809
AlwI GGATC 1 cut(s) 325
AlwNI CAGNNNCTG 1 cut(s) 208
Ama87I CYCGRG 1 cut(s) 812
AoxI GGCC 3 cut(s) 115, 194, 570
ApaI GGGCCC 1 cut(s) 198
ApeKI GCWGC 2 cut(s) 449, 668
ApoI RAATTY 1 cut(s) 557
Asp700I GAANNNNTTC 1 cut(s) 534
AspS9I GGNCC 6 cut(s) 115, 194, 195, 432, 570, 607
AsuHPI GGTGA 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 812
AvaII GGWCC 2 cut(s) 432, 607
BaeGI GKGCMC 1 cut(s) 198
BaeI ACNNNNGTAYC 2 cut(s) 138, 171
BanII GRGCYC 2 cut(s) 198, 748
BbvI GCAGC 2 cut(s) 461, 655
BccI CCATC 5 cut(s) 319, 619, 747, 796, 881
BciT130I CCWGG 1 cut(s) 203
BciVI GTATCC 2 cut(s) 596, 814
BclI TGATCA 2 cut(s) 102, 841
BcoDI GTCTC 3 cut(s) 407, 461, 809
BfaI CTAG 1 cut(s) 771
BfmI CTRYAG 1 cut(s) 271
BfrI CTTAAG 1 cut(s) 425
BfuAI ACCTGC 1 cut(s) 443
BfuI GTATCC 2 cut(s) 596, 814
BisI GCNGC 3 cut(s) 367, 450, 669
BlpI GCTNAGC 1 cut(s) 488
BlsI GCNGC 3 cut(s) 368, 451, 670
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 2 cut(s) 432, 607
BmeRI GACNNNNNGTC 1 cut(s) 420
BmeT110I CYCGRG 1 cut(s) 812
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 6 cut(s) 115, 194, 195, 432, 570, 607
BmiI GGNNCC 3 cut(s) 196, 197, 609
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 698
BpmI CTGGAG 1 cut(s) 185
Bpu1102I GCTNAGC 1 cut(s) 488
BpuEI CTTGAG 2 cut(s) 311, 843
BsaBI GATNNNNATC 1 cut(s) 852
BsaI GGTCTC 2 cut(s) 407, 461
BsaJI CCNNGG 2 cut(s) 202, 334
BsaXI ACNNNNNCTCC 4 cut(s) 268, 298, 519, 549
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 500
Bse8I GATNNNNATC 1 cut(s) 852
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 202, 334
BseGI GGATG 1 cut(s) 853
BseJI GATNNNNATC 1 cut(s) 852
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 500
BseSI GKGCMC 1 cut(s) 198
BseXI GCAGC 2 cut(s) 461, 655
BshFI GGCC 3 cut(s) 117, 196, 572
BsiHKCI CYCGRG 1 cut(s) 812
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 3 cut(s) 407, 461, 809
BsmBI CGTCTC 1 cut(s) 809
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 3 cut(s) 117, 196, 572
Bso31I GGTCTC 2 cut(s) 407, 461
BsoBI CYCGRG 1 cut(s) 812
Bsp120I GGGCCC 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 198, 748
Bsp1407I TGTACA 1 cut(s) 179
Bsp143I GATC 4 cut(s) 102, 259, 330, 841
Bsp1720I GCTNAGC 1 cut(s) 488
BspACI CCGC 2 cut(s) 367, 499
BspANI GGCC 3 cut(s) 117, 196, 572
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 3 cut(s) 196, 197, 609
BspMI ACCTGC 1 cut(s) 443
BspPI GGATC 1 cut(s) 325
BspTI CTTAAG 1 cut(s) 425
BspTNI GGTCTC 2 cut(s) 407, 461
BsrGI TGTACA 1 cut(s) 179
BsrI ACTGG 1 cut(s) 500
BssECI CCNNGG 2 cut(s) 202, 334
BssMI GATC 4 cut(s) 102, 259, 330, 841
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 78, 210
BstAFI CTTAAG 1 cut(s) 425
BstAUI TGTACA 1 cut(s) 179
BstDEI CTNAG 4 cut(s) 150, 488, 699, 891
BstF5I GGATG 1 cut(s) 853
BstKTI GATC 4 cut(s) 105, 262, 333, 844
BstMAI GTCTC 3 cut(s) 407, 461, 809
BstMBI GATC 4 cut(s) 102, 259, 330, 841
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 180
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 1 cut(s) 271
BstSLI GKGCMC 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 461, 655
BstX2I RGATCY 1 cut(s) 330
BstYI RGATCY 1 cut(s) 330
BsuI GTATCC 2 cut(s) 596, 814
BsuRI GGCC 3 cut(s) 117, 196, 572
BtrI CACGTC 1 cut(s) 478
BtsCI GGATG 1 cut(s) 853
BtsIMutI CAGTG 1 cut(s) 493
BveI ACCTGC 1 cut(s) 443
CaiI CAGNNNCTG 1 cut(s) 208
Cfr13I GGNCC 6 cut(s) 115, 194, 195, 432, 570, 607
CseI GACGC 1 cut(s) 684
Csp6I GTAC 3 cut(s) 147, 180, 598
CviAII CATG 4 cut(s) 177, 242, 280, 539
CviQI GTAC 3 cut(s) 147, 180, 598
DdeI CTNAG 4 cut(s) 150, 488, 699, 891
DpnI GATC 4 cut(s) 104, 261, 332, 843
DpnII GATC 4 cut(s) 102, 259, 330, 841
DriI GACNNNNNGTC 1 cut(s) 420
Eam1105I GACNNNNNGTC 1 cut(s) 420
Eco24I GRGCYC 2 cut(s) 198, 748
Eco31I GGTCTC 2 cut(s) 407, 461
Eco47I GGWCC 2 cut(s) 432, 607
Eco88I CYCGRG 1 cut(s) 812
EcoO109I RGGNCCY 2 cut(s) 195, 607
EcoRII CCWGG 1 cut(s) 201
EcoT38I GRGCYC 2 cut(s) 198, 748
Esp3I CGTCTC 1 cut(s) 809
FaeI CATG 4 cut(s) 180, 245, 283, 542
FaqI GGGAC 1 cut(s) 219
FatI CATG 4 cut(s) 176, 241, 279, 538
FbaI TGATCA 2 cut(s) 102, 841
Fnu4HI GCNGC 3 cut(s) 367, 450, 669
FokI GGATG 1 cut(s) 840
FriOI GRGCYC 2 cut(s) 198, 748
Fsp4HI GCNGC 3 cut(s) 367, 450, 669
FspBI CTAG 1 cut(s) 771
GluI GCNGC 3 cut(s) 367, 450, 669
GsuI CTGGAG 1 cut(s) 185
HaeIII GGCC 3 cut(s) 117, 196, 572
HgaI GACGC 1 cut(s) 684
Hin1II CATG 4 cut(s) 180, 245, 283, 542
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 58
HinfI GANTC 5 cut(s) 8, 17, 131, 253, 887
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 4 cut(s) 91, 267, 432, 598
Hpy188I TCNGA 2 cut(s) 335, 883
Hpy188III TCNNGA 6 cut(s) 257, 328, 712, 797, 845, 860
Hpy8I GTNNAC 4 cut(s) 91, 267, 432, 598
HpyAV CCTTC 3 cut(s) 518, 598, 660
HpyCH4III ACNGT 2 cut(s) 78, 210
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 2 cut(s) 36, 449
HpyF3I CTNAG 4 cut(s) 150, 488, 699, 891
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 4 cut(s) 180, 245, 283, 542
Ksp22I TGATCA 2 cut(s) 102, 841
Kzo9I GATC 4 cut(s) 102, 259, 330, 841
LmnI GCTCC 1 cut(s) 276
Lsp1109I GCAGC 2 cut(s) 461, 655
LweI GCATC 1 cut(s) 698
MaeI CTAG 1 cut(s) 771
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 1 cut(s) 734
MalI GATC 4 cut(s) 104, 261, 332, 843
MboI GATC 4 cut(s) 102, 259, 330, 841
MboII GAAGA 2 cut(s) 635, 645
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 2 cut(s) 198, 748
MluCI AATT 6 cut(s) 41, 124, 515, 557, 707, 764
MmeI TCCRAC 1 cut(s) 661
MnlI CCTC 7 cut(s) 145, 209, 329, 514, 547, 768, 867
MroXI GAANNNNTTC 1 cut(s) 534
MseI TTAA 4 cut(s) 123, 341, 399, 426
MslI CAYNNNNRTG 1 cut(s) 98
MspCI CTTAAG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 203
MvaI CCWGG 1 cut(s) 203
NdeII GATC 4 cut(s) 102, 259, 330, 841
NlaIII CATG 4 cut(s) 180, 245, 283, 542
NlaIV GGNNCC 3 cut(s) 196, 197, 609
NspI RCATGY 1 cut(s) 180
PciI ACATGT 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 534
PfeI GAWTC 5 cut(s) 8, 17, 131, 253, 887
PkrI GCNGC 3 cut(s) 368, 451, 670
PpuMI RGGWCCY 1 cut(s) 607
PscI ACATGT 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 607
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 3 cut(s) 196, 197, 609
PspOMI GGGCCC 1 cut(s) 194
PspPI GGNCC 6 cut(s) 115, 194, 195, 432, 570, 607
PspPPI RGGWCCY 1 cut(s) 607
PstNI CAGNNNCTG 1 cut(s) 208
PsuI RGATCY 1 cut(s) 330
RsaI GTAC 3 cut(s) 148, 181, 599
RsaNI GTAC 3 cut(s) 147, 180, 598
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 4 cut(s) 123, 341, 399, 426
SatI GCNGC 3 cut(s) 367, 450, 669
Sau3AI GATC 4 cut(s) 102, 259, 330, 841
Sau96I GGNCC 6 cut(s) 115, 194, 195, 432, 570, 607
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 2 cut(s) 198, 748
SfaNI GCATC 1 cut(s) 698
SfcI CTRYAG 1 cut(s) 271
SinI GGWCC 2 cut(s) 432, 607
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 3 cut(s) 326, 425, 858
SmoI CTYRAG 3 cut(s) 326, 425, 858
Sse9I AATT 6 cut(s) 41, 124, 515, 557, 707, 764
SsiI CCGC 2 cut(s) 367, 499
SspMI CTAG 1 cut(s) 771
StyD4I CCNGG 1 cut(s) 201
TaaI ACNGT 2 cut(s) 78, 210
TaiI ACGT 1 cut(s) 480
TaqI TCGA 1 cut(s) 545
TasI AATT 6 cut(s) 41, 124, 515, 557, 707, 764
TatI WGTACW 1 cut(s) 179
TauI GCSGC 1 cut(s) 369
TfiI GAWTC 5 cut(s) 8, 17, 131, 253, 887
Tru1I TTAA 4 cut(s) 123, 341, 399, 426
Tru9I TTAA 4 cut(s) 123, 341, 399, 426
TscAI CASTG 1 cut(s) 500
TseI GCWGC 2 cut(s) 449, 668
TspDTI ATGAA 8 cut(s) 228, 230, 527, 528, 743, 797, 818, 842
TspGWI ACGGA 1 cut(s) 833
TspRI CASTG 1 cut(s) 500
Vha464I CTTAAG 1 cut(s) 425
VpaK11BI GGWCC 2 cut(s) 432, 607
XapI RAATTY 1 cut(s) 557
XceI RCATGY 1 cut(s) 180
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.