pycom15g33620

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000654
Physical Location & Seq
Forward (+)
33078803 .. 33082651
3849 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33620.4

Sequence Viewer

Length: 3342 bp
ATGGCTGCAATGCGCACCGAGCAACAGAATGCTTCTTCGTCCTCTTACCGGTGCACGTATGATGCATTCTTGAGTTTCAGAGGCACAGATACACGCAAGGGCTTTACAGATCACCTCTACAGTGCTTTGGAGTGGGCAGGAATCCACACTTTTAGGGACGATGAAGAAATTGAGACAGGAGAAAACATTGAACAGGAGCTACGGACGGCAATACAAGAGTCACGAGTATCAATCATTGTTTTCTCCAAGGACTACGCCTCTTCCAGTTGGTGCCTGAATGAACTTGCTATCATCATGGAACGTAAAAGAACAGATGGACACATGGTTATCCCAGTTTTCTATGATGTGGAACCATCAGATGTCAGGAAGCAGACGGGCAGTTTTGCAGAATCATTTACTAAACATGAAAAGCGCTTCAAGGACAAGGTGGAGGAGTGGAAGCGAGCTCTTAGAGATGTTGCAGATTTGGCAGGGATGGTTTATAGAGAACAGTACGAGTCGCAGTTTATCCAAGATATTGTTGAAGCGATTGGAAATAAGCTGAACGACATGACGAATACGAGATTAAGAGTTGAGCCCAATGTGATTGGAAGAGATTATCATGTGAAAAGACTAAACATGTGGCTGGAAGATGGATCAAATGATGTTGGAGTATCTGTCATCTATGGAATGGGTGGAATAGGCAAGACCACCATTGCTAAAACTGTTTATAACCAGAACTCCGATAAATTTCAAGCTAGCAGCTTTCTTTCAGATATTTGGGAAACTTCCAAACAAGGCAATGGTATTGTTCACTTGCAAAGGAAGCTTCTTTCAGATATCCAAAAGGGGAAAATGGAAAAAATATACAGCCTTGATGAAGGTATGGTAAAGATCGAACGGGTTGTACGTTGCAAAAGAGTTCTTATTGTTCTTGATGATGTGGAGAACTCAGAACAGTTCAATGCAATTCTTGGAATGCAAGACTGGTTCCATCCTGGAAGTAAAATTATCATAACAACTAGACATGAACATTTGTTAAAGGCTCATGAAGTTTGTGTAAGGTTTAAGGTTGAAGGATTACATGCGCATCAATCTCTTATGCTTTTCAGTTGGCATGCCTTCGGACAACCCCATCCTCAGGAAGGTTACATGGAGCATTCAAGAACCTTAGTAAAACATTGTGGAGGGGTTCCATTAGCTCTTCAAGTTCTGGGATCTTCTCTATTTGAAAAAACAGCAAATGTATGGGAAAATGTAGTACGCAACTTAGATGTTTTAGACGTGATTACTGAGGGAAAAATTAAAAAGATTCTTTGCAAAAGTTTTGAATCTTTACAAGATCATGATAGACGTTTATTCCTCCATATAGCCTGTTTCTTCGTAGGAAAGGACAAGGATTTCGCAACTACAATCCTTGACAAATGTGATTTTGTAACAGAGATTGGACTTCAACATCTAGTTGATAGATGCCTTTTGGAAATTGATGCCCACAGCAAGTTAACCATGCATCAATTGCTTCAAGACATGGGAAGGGCAATAATTCACGACCAATCACCTGAGGACCCTGGAAAACGCACTAGACTGTGGGATAAAGATGCAATTAACGTTTTGAGAAAATTAACTGGTACGAGAACTATCAAGGGCCTCATGCTCAACTTTCCCTCAACAAAGTCATCGCCGGTATTAAATGAGGTTCGTTTCGAAACAAAGGCATTTACAGAGATGCTCAATCTTGAACTACTTCTGCTTGATAATGTAAAGTTGAGTGGAAGCTATGAAGATTTTCCAAAAAATTTAATATGGCTGTCTTGGCGAGGATTCTCTTTAAAATCAATACCATCCAATTTTTGTATGGAACATCTAGTTGTTCTTGACTTACGGTACAGCAGTCTGCAACATATTTGGAAAGGAACCAAGATTCTTACAAGATTGAAAATCCTTAATCTCAGTCATTCACATGGTCTTAGGACAACCTCTGACTTGTCTGGATTCCCTAACCTTGAGAAACTAATTCTTAAGGATTGCATTAATTTGGTTGATGTTGATGAATCCATTGGAAACTTGGGGAAACTTGTTTTCTTGAATCTAAAAGACTGCAAAAGCCTTATGAAGCTTCCAAAAAGAATGAACTGGTTGAGGTCTCTTGAGGAACTTATTCTTAGCGGTTGCTCAAAGCTTGTGCTTCATGACAGTGCCACTGTAATTCATGTACACGCGATATCTGGGGATATGAATAAACCTAGACTGTTGTCAACTTTATCATGGACACCAATCAGGTCTTGGTGGATGTGGGCATGGCCGGGAAAGACTCTTCAATCAACCAGTTTCTCACTGGCAAGTTTACCGCGTTGTTTGGGAAGCTTAAATCTGTCTCGTTGCAATCTGTCAGAGGTTCCCAATGATCTGTGTTCAATATCTTCATTGAAGTATTTGGATCTAAGTAGAAACCCAATTTTGTGCCTACCACAAAACATGAAGAGTCTTATTATGCTCGAGACTCTTTTGTTATATAAGTGCACAAACCTCGAAATGCTTCCAGAGCTCCCAGCAAGGTTGAGAAGTTTGCAAGCAGCTTATTGTACATCATTGAAAAAATTAACAAATTTACCAAACTTGTTCAAATCATCGGAAATATCTTTTTGGCGTTGCCCTCGGTTAGTTGAAGTTGAAAGCTTGTTGAATATAAAAACGTTGAGAAGCGCTGACATAGAAATGACAAGATATATGGGACTGTTCGATTTGAAATCCATAGCAAGCACTGATGTCGAAATGCTCAACCACTTGACCAGTACTACCAGGAAGGTTCCTGTCCAGGCAGTCTATGAGTGTGGCATATATAGCATATTCCTTCATGCAAGCAAGATTCCAGATGGATTTGACTCCACAATACAGACATCTGTGCTGTCAGTTATTGCACCTTCACATCCTAATCTCAAAATCCAAGGCTTGAATGTAGGCGTTTTATATGCCAAAAATGAACATCAGATTGAAGCCACTGTAGAAGCTGTGATTGAAGTTAGTAATCAGACGAAGGCTCTCATGTGGACCTATTGCTCACTCACAATAGGTTTGCCAACTGAGAAGGAAGATGTGTTGTGGCTTAGCCATTTTCGATTTGGAAACAACGAATTGGAGGATGGGGACCAAATACGTGTTTCGGTGGAGATGGGTGCTTTTTGTCGGGTGAAGGAGTTCGGAATCCAGCTTGTGTACGAGCCGGAAAGACCTCCTTCTAGCCAGAATAATGTTGTTGCTGGAGATGTGTCACTGTCAGCATCAGAGTACCAAATGGGGACAGGAAAATACTTTCTCAGTATTTTTGGGCACCGTTCTCGGCCTTCGTGGGCACCGTTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1114

Amino Acids

126.7

Weight (kDa)

7.21

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 711
Acc16I TGCGCA 2 cut(s) 14, 1068
AccB1I GGYRCC 3 cut(s) 270, 3306, 3328
AccII CGCG 2 cut(s) 2198, 2329
AciI CCGC 2 cut(s) 2145, 2327
AclI AACGTT 2 cut(s) 1587, 2672
AclWI GGATC 3 cut(s) 643, 1204, 2424
AcoI YGGCCR 1 cut(s) 2279
AcsI RAATTY 3 cut(s) 728, 1774, 2584
AfeI AGCGCT 2 cut(s) 413, 2683
AfiI CCNNNNNNNGG 3 cut(s) 48, 1120, 1124
AflII CTTAAG 1 cut(s) 1997
AflIII ACRYGT 2 cut(s) 618, 3133
AgeI ACCGGT 1 cut(s) 48
AjiI CACGTC 1 cut(s) 1264
AjnI CCWGG 4 cut(s) 976, 1546, 2777, 2793
Alw21I GWGCWC 4 cut(s) 56, 448, 2501, 2526
Alw26I GTCTC 4 cut(s) 167, 2127, 2358, 2471
Alw44I GTGCAC 2 cut(s) 52, 2497
AlwI GGATC 3 cut(s) 643, 1204, 2424
Ama87I CYCGRG 1 cut(s) 2474
Aor51HI AGCGCT 2 cut(s) 413, 2683
AoxI GGCC 3 cut(s) 1624, 2279, 3317
ApaLI GTGCAC 2 cut(s) 52, 2497
ApeKI GCWGC 3 cut(s) 5, 741, 2552
ApoI RAATTY 3 cut(s) 728, 1774, 2584
ArsI GACNNNNNNTTYG 2 cut(s) 1364, 1396
AseI ATTAAT 1 cut(s) 2010
AsiGI ACCGGT 1 cut(s) 48
Asp700I GAANNNNTTC 5 cut(s) 1722, 1764, 2136, 2514, 3173
AspLEI GCGC 4 cut(s) 15, 414, 1069, 2684
AspS9I GGNCC 4 cut(s) 1543, 1624, 3027, 3124
AsuC2I CCSGG 1 cut(s) 2283
AsuHPI GGTGA 3 cut(s) 104, 1527, 3178
AsuII TTCGAA 1 cut(s) 1683
AsuNHI GCTAGC 1 cut(s) 737
AvaI CYCGRG 1 cut(s) 2474
AvaII GGWCC 3 cut(s) 1543, 3027, 3124
AxyI CCTNAGG 2 cut(s) 1119, 1539
BaeGI GKGCMC 4 cut(s) 56, 2501, 3309, 3331
BaeI ACNNNNGTAYC 2 cut(s) 1855, 1888
BanI GGYRCC 3 cut(s) 270, 3306, 3328
BanII GRGCYC 3 cut(s) 448, 579, 2526
BauI CACGAG 1 cut(s) 222
Bbv12I GWGCWC 4 cut(s) 56, 448, 2501, 2526
BbvI GCAGC 2 cut(s) 753, 2564
BceAI ACGGC 1 cut(s) 222
BcgI CGANNNNNNTGC 6 cut(s) 2488, 2522, 2728, 2762, 3296, 3330
BciT130I CCWGG 4 cut(s) 978, 1548, 2779, 2795
BcnI CCSGG 1 cut(s) 2283
BcoDI GTCTC 4 cut(s) 167, 2127, 2358, 2471
BfaI CTAG 7 cut(s) 738, 1002, 1439, 1560, 1844, 2223, 3216
BfmI CTRYAG 2 cut(s) 118, 2979
BfoI RGCGCY 2 cut(s) 415, 2685
BfrI CTTAAG 1 cut(s) 1997
BisI GCNGC 3 cut(s) 6, 742, 2553
BlpI GCTNAGC 1 cut(s) 3083
BlsI GCNGC 3 cut(s) 7, 743, 2554
BmcAI AGTACT 1 cut(s) 2773
Bme1390I CCNGG 5 cut(s) 978, 1548, 2283, 2779, 2795
Bme18I GGWCC 3 cut(s) 1543, 3027, 3124
BmeT110I CYCGRG 1 cut(s) 2474
BmgBI CACGTC 1 cut(s) 1264
BmgT120I GGNCC 4 cut(s) 1543, 1624, 3027, 3124
BmrFI CCNGG 5 cut(s) 978, 1548, 2283, 2779, 2795
BmrI ACTGGG 1 cut(s) 326
BmsI GCATC 8 cut(s) 52, 1078, 1439, 1456, 1498, 1567, 1695, 3266
BmtI GCTAGC 1 cut(s) 741
BmuI ACTGGG 1 cut(s) 326
BoxI GACNNNNGTC 1 cut(s) 2230
BpmI CTGGAG 1 cut(s) 3258
Bpu1102I GCTNAGC 1 cut(s) 3083
Bpu14I TTCGAA 1 cut(s) 1683
BpuEI CTTGAG 3 cut(s) 91, 2003, 2147
BpuMI CCSGG 1 cut(s) 2283
BsaAI YACGTR 2 cut(s) 57, 3134
BsaI GGTCTC 1 cut(s) 2127
BsaJI CCNNGG 4 cut(s) 246, 1546, 2633, 2923
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 4 cut(s) 642, 672, 704, 734
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 1120, 1124
Bse118I RCCGGY 2 cut(s) 48, 1660
Bse1I ACTGG 8 cut(s) 264, 332, 971, 1609, 2117, 2304, 2319, 2769
Bse21I CCTNAGG 2 cut(s) 1119, 1539
Bse3DI GCAATG 3 cut(s) 15, 693, 787
BseBI CCWGG 4 cut(s) 978, 1548, 2779, 2795
BseDI CCNNGG 4 cut(s) 246, 1546, 2633, 2923
BseGI GGATG 7 cut(s) 480, 973, 1114, 1820, 2274, 2905, 3124
BseLI CCNNNNNNNGG 3 cut(s) 48, 1120, 1124
BseMI GCAATG 3 cut(s) 15, 693, 787
BseMII CTCAG 7 cut(s) 945, 1133, 1263, 1530, 1942, 3051, 3307
BseNI ACTGG 8 cut(s) 264, 332, 971, 1609, 2117, 2304, 2319, 2769
BseRI GAGGAG 1 cut(s) 446
BseSI GKGCMC 4 cut(s) 56, 2501, 3309, 3331
BseXI GCAGC 2 cut(s) 753, 2564
BseYI CCCAGC 1 cut(s) 2527
Bsh1236I CGCG 2 cut(s) 2198, 2329
BshFI GGCC 3 cut(s) 1626, 2281, 3319
BshNI GGYRCC 3 cut(s) 270, 3306, 3328
BshTI ACCGGT 1 cut(s) 48
BsiHKAI GWGCWC 4 cut(s) 56, 448, 2501, 2526
BsiHKCI CYCGRG 1 cut(s) 2474
BsiSI CCGG 4 cut(s) 49, 1661, 2282, 3200
BslFI GGGAC 4 cut(s) 170, 2724, 3137, 3289
BslI CCNNNNNNNGG 3 cut(s) 48, 1120, 1124
BsmAI GTCTC 4 cut(s) 167, 2127, 2358, 2471
BsmFI GGGAC 4 cut(s) 170, 2724, 3137, 3289
BsmI GAATGC 4 cut(s) 34, 65, 963, 1138
BsnI GGCC 3 cut(s) 1626, 2281, 3319
Bso31I GGTCTC 1 cut(s) 2127
BsoBI CYCGRG 1 cut(s) 2474
Bsp119I TTCGAA 1 cut(s) 1683
Bsp1286I GDGCHC 7 cut(s) 56, 448, 579, 2501, 2526, 3309, 3331
Bsp1407I TGTACA 2 cut(s) 2191, 2561
Bsp143I GATC 7 cut(s) 109, 635, 873, 1196, 1321, 2383, 2416
Bsp1720I GCTNAGC 1 cut(s) 3083
BspACI CCGC 2 cut(s) 2145, 2327
BspANI GGCC 3 cut(s) 1626, 2281, 3319
BspCNI CTCAG 7 cut(s) 944, 1132, 1264, 1531, 1941, 3052, 3306
BspFNI CGCG 2 cut(s) 2198, 2329
BspHI TCATGA 4 cut(s) 1027, 1324, 2167, 3338
BspOI GCTAGC 1 cut(s) 741
BspPI GGATC 3 cut(s) 643, 1204, 2424
BspQI GCTCTTC 1 cut(s) 1188
BspT104I TTCGAA 1 cut(s) 1683
BspT107I GGYRCC 3 cut(s) 270, 3306, 3328
BspTI CTTAAG 1 cut(s) 1997
BspTNI GGTCTC 1 cut(s) 2127
BsrDI GCAATG 3 cut(s) 15, 693, 787
BsrFI RCCGGY 2 cut(s) 48, 1660
BsrGI TGTACA 2 cut(s) 2191, 2561
BsrI ACTGG 8 cut(s) 264, 332, 971, 1609, 2117, 2304, 2319, 2769
BssAI RCCGGY 2 cut(s) 48, 1660
BssECI CCNNGG 4 cut(s) 246, 1546, 2633, 2923
BssMI GATC 7 cut(s) 109, 635, 873, 1196, 1321, 2383, 2416
BssSI CACGAG 1 cut(s) 222
BssT1I CCWWGG 2 cut(s) 246, 2923
Bst2BI CACGAG 1 cut(s) 222
Bst2UI CCWGG 4 cut(s) 978, 1548, 2779, 2795
Bst6I CTCTTC 5 cut(s) 265, 586, 1188, 2298, 2453
BstAFI CTTAAG 1 cut(s) 1997
BstAPI GCANNNNNTGC 1 cut(s) 1495
BstAUI TGTACA 2 cut(s) 2191, 2561
BstBAI YACGTR 2 cut(s) 57, 3134
BstBI TTCGAA 1 cut(s) 1683
BstC8I GCNNGC 6 cut(s) 444, 739, 1098, 2550, 2737, 2839
BstENI CCTNNNNNAGG 1 cut(s) 1122
BstF5I GGATG 7 cut(s) 480, 973, 1114, 1820, 2274, 2905, 3124
BstFNI CGCG 2 cut(s) 2198, 2329
BstH2I RGCGCY 2 cut(s) 415, 2685
BstHHI GCGC 4 cut(s) 15, 414, 1069, 2684
BstKTI GATC 7 cut(s) 112, 638, 876, 1199, 1324, 2386, 2419
BstMAI GTCTC 4 cut(s) 167, 2127, 2358, 2471
BstMBI GATC 7 cut(s) 109, 635, 873, 1196, 1321, 2383, 2416
BstMWI GCNNNNNNNGC 7 cut(s) 19, 467, 805, 1495, 1792, 2521, 2820
BstNI CCWGG 4 cut(s) 978, 1548, 2779, 2795
BstNSI RCATGY 3 cut(s) 622, 1067, 1100
BstPAI GACNNNNGTC 1 cut(s) 2230
BstSCI CCNGG 5 cut(s) 976, 1546, 2281, 2777, 2793
BstSFI CTRYAG 2 cut(s) 118, 2979
BstSLI GKGCMC 4 cut(s) 56, 2501, 3309, 3331
BstUI CGCG 2 cut(s) 2198, 2329
BstV1I GCAGC 2 cut(s) 753, 2564
BstX2I RGATCY 2 cut(s) 1196, 2416
BstYI RGATCY 2 cut(s) 1196, 2416
Bsu36I CCTNAGG 2 cut(s) 1119, 1539
BsuRI GGCC 3 cut(s) 1626, 2281, 3319
BtgZI GCGATG 1 cut(s) 1641
BtrI CACGTC 1 cut(s) 1264
BtsCI GGATG 7 cut(s) 480, 973, 1114, 1820, 2274, 2905, 3124
BtsIMutI CAGTG 7 cut(s) 127, 2178, 2179, 2312, 2739, 2976, 3248
Cac8I GCNNGC 6 cut(s) 444, 739, 1098, 2550, 2737, 2839
CciI TCATGA 4 cut(s) 1027, 1324, 2167, 3338
CfoI GCGC 4 cut(s) 15, 414, 1069, 2684
Cfr10I RCCGGY 2 cut(s) 48, 1660
Cfr13I GGNCC 4 cut(s) 1543, 1624, 3027, 3124
CspAI ACCGGT 1 cut(s) 48
DpnI GATC 7 cut(s) 111, 637, 875, 1198, 1323, 2385, 2418
DpnII GATC 7 cut(s) 109, 635, 873, 1196, 1321, 2383, 2416
DraI TTTAAA 1 cut(s) 1809
EaeI YGGCCR 1 cut(s) 2279
Eam1104I CTCTTC 5 cut(s) 265, 586, 1188, 2298, 2453
EarI CTCTTC 5 cut(s) 265, 586, 1188, 2298, 2453
Ecl136II GAGCTC 2 cut(s) 446, 2524
Eco130I CCWWGG 2 cut(s) 246, 2923
Eco24I GRGCYC 3 cut(s) 448, 579, 2526
Eco31I GGTCTC 1 cut(s) 2127
Eco32I GATATC 2 cut(s) 820, 2202
Eco47I GGWCC 3 cut(s) 1543, 3027, 3124
Eco47III AGCGCT 2 cut(s) 413, 2683
Eco53kI GAGCTC 2 cut(s) 446, 2524
Eco81I CCTNAGG 2 cut(s) 1119, 1539
Eco88I CYCGRG 1 cut(s) 2474
EcoICRI GAGCTC 2 cut(s) 446, 2524
EcoNI CCTNNNNNAGG 1 cut(s) 1122
EcoO109I RGGNCCY 2 cut(s) 1543, 1624
EcoRII CCWGG 4 cut(s) 976, 1546, 2777, 2793
EcoRV GATATC 2 cut(s) 820, 2202
EcoT14I CCWWGG 2 cut(s) 246, 2923
EcoT22I ATGCAT 2 cut(s) 67, 1491
EcoT38I GRGCYC 3 cut(s) 448, 579, 2526
ErhI CCWWGG 2 cut(s) 246, 2923
FalI AAGNNNNNCTT 2 cut(s) 3196, 3228
FaqI GGGAC 4 cut(s) 170, 2724, 3137, 3289
Fnu4HI GCNGC 3 cut(s) 6, 742, 2553
FokI GGATG 7 cut(s) 487, 960, 1101, 1807, 2281, 2892, 3131
FriOI GRGCYC 3 cut(s) 448, 579, 2526
Fsp4HI GCNGC 3 cut(s) 6, 742, 2553
FspAI RTGCGCAY 2 cut(s) 14, 1068
FspBI CTAG 7 cut(s) 738, 1002, 1439, 1560, 1844, 2223, 3216
FspI TGCGCA 2 cut(s) 14, 1068
GlaI GCGC 4 cut(s) 14, 413, 1068, 2683
GluI GCNGC 3 cut(s) 6, 742, 2553
GsaI CCCAGC 1 cut(s) 2531
GsuI CTGGAG 1 cut(s) 3258
HaeII RGCGCY 2 cut(s) 415, 2685
HaeIII GGCC 3 cut(s) 1626, 2281, 3319
HapII CCGG 4 cut(s) 49, 1661, 2282, 3200
HhaI GCGC 4 cut(s) 15, 414, 1069, 2684
Hin6I GCGC 4 cut(s) 13, 412, 1067, 2682
HinP1I GCGC 4 cut(s) 13, 412, 1067, 2682
HincII GTYRAC 2 cut(s) 1482, 2235
HindII GTYRAC 2 cut(s) 1482, 2235
HindIII AAGCTT 5 cut(s) 806, 2093, 2156, 2341, 2653
HpaI GTTAAC 1 cut(s) 1482
HpaII CCGG 4 cut(s) 49, 1661, 2282, 3200
HphI GGTGA 3 cut(s) 104, 1527, 3178
Hpy166II GTNNAC 9 cut(s) 54, 793, 1482, 2194, 2235, 2324, 2499, 3027, 3193
Hpy8I GTNNAC 9 cut(s) 54, 793, 1482, 2194, 2235, 2324, 2499, 3027, 3193
HpyCH4IV ACGT 8 cut(s) 56, 301, 889, 1263, 1333, 1587, 2672, 3133
HpyF10VI GCNNNNNNNGC 7 cut(s) 19, 467, 805, 1495, 1792, 2521, 2820
HpySE526I ACGT 8 cut(s) 56, 301, 889, 1263, 1333, 1587, 2672, 3133
HspAI GCGC 4 cut(s) 13, 412, 1067, 2682
KspAI GTTAAC 1 cut(s) 1482
Kzo9I GATC 7 cut(s) 109, 635, 873, 1196, 1321, 2383, 2416
LguI GCTCTTC 1 cut(s) 1188
LmnI GCTCC 3 cut(s) 196, 1135, 2529
Lsp1109I GCAGC 2 cut(s) 753, 2564
LweI GCATC 8 cut(s) 52, 1078, 1439, 1456, 1498, 1567, 1695, 3266
MaeI CTAG 7 cut(s) 738, 1002, 1439, 1560, 1844, 2223, 3216
MaeII ACGT 8 cut(s) 56, 301, 889, 1263, 1333, 1587, 2672, 3133
MaeIII GTNAC 4 cut(s) 219, 1127, 1414, 3246
MalI GATC 7 cut(s) 111, 637, 875, 1198, 1323, 2385, 2418
MboI GATC 7 cut(s) 109, 635, 873, 1196, 1321, 2383, 2416
MfeI CAATTG 1 cut(s) 1493
MflI RGATCY 2 cut(s) 1196, 2416
MhlI GDGCHC 7 cut(s) 56, 448, 579, 2501, 2526, 3309, 3331
MlyI GAGTC 6 cut(s) 227, 506, 2284, 2470, 2473, 2855
MmeI TCCRAC 1 cut(s) 628
Mph1103I ATGCAT 2 cut(s) 67, 1491
MroXI GAANNNNTTC 5 cut(s) 1722, 1764, 2136, 2514, 3173
MslI CAYNNNNRTG 3 cut(s) 1938, 2172, 2693
MspCI CTTAAG 1 cut(s) 1997
MspI CCGG 4 cut(s) 49, 1661, 2282, 3200
MspR9I CCNGG 5 cut(s) 978, 1548, 2283, 2779, 2795
MunI CAATTG 1 cut(s) 1493
Mva1269I GAATGC 4 cut(s) 34, 65, 963, 1138
MvaI CCWGG 4 cut(s) 978, 1548, 2779, 2795
MvnI CGCG 2 cut(s) 2198, 2329
MwoI GCNNNNNNNGC 7 cut(s) 19, 467, 805, 1495, 1792, 2521, 2820
NciI CCSGG 1 cut(s) 2283
NdeII GATC 7 cut(s) 109, 635, 873, 1196, 1321, 2383, 2416
NheI GCTAGC 1 cut(s) 737
NmeAIII GCCGAG 1 cut(s) 3295
NmuCI GTSAC 2 cut(s) 219, 3246
NsbI TGCGCA 2 cut(s) 14, 1068
NsiI ATGCAT 2 cut(s) 67, 1491
NspI RCATGY 3 cut(s) 622, 1067, 1100
NspV TTCGAA 1 cut(s) 1683
PaeI GCATGC 1 cut(s) 1100
PaeR7I CTCGAG 1 cut(s) 2474
PagI TCATGA 4 cut(s) 1027, 1324, 2167, 3338
PciI ACATGT 1 cut(s) 618
PciSI GCTCTTC 1 cut(s) 1188
PcsI WCGNNNNNNNCGW 2 cut(s) 886, 3329
PctI GAATGC 4 cut(s) 34, 65, 963, 1138
PdmI GAANNNNTTC 5 cut(s) 1722, 1764, 2136, 2514, 3173
PfoI TCCNGGA 1 cut(s) 976
PinAI ACCGGT 1 cut(s) 48
PkrI GCNGC 3 cut(s) 7, 743, 2554
PleI GAGTC 6 cut(s) 226, 505, 2284, 2469, 2473, 2855
PpsI GAGTC 6 cut(s) 226, 505, 2284, 2469, 2473, 2855
Ppu21I YACGTR 2 cut(s) 57, 3134
PpuMI RGGWCCY 1 cut(s) 1543
PscI ACATGT 1 cut(s) 618
PshAI GACNNNNGTC 1 cut(s) 2230
PshBI ATTAAT 1 cut(s) 2010
PsiI TTATAA 1 cut(s) 711
Psp124BI GAGCTC 2 cut(s) 448, 2526
Psp1406I AACGTT 2 cut(s) 1587, 2672
Psp5II RGGWCCY 1 cut(s) 1543
Psp6I CCWGG 4 cut(s) 976, 1546, 2777, 2793
PspFI CCCAGC 1 cut(s) 2527
PspGI CCWGG 4 cut(s) 976, 1546, 2777, 2793
PspPI GGNCC 4 cut(s) 1543, 1624, 3027, 3124
PspPPI RGGWCCY 1 cut(s) 1543
PsrI GAACNNNNNNTAC 4 cut(s) 183, 215, 870, 902
PsuI RGATCY 2 cut(s) 1196, 2416
RseI CAYNNNNRTG 3 cut(s) 1938, 2172, 2693
SacI GAGCTC 2 cut(s) 448, 2526
SapI GCTCTTC 1 cut(s) 1188
SatI GCNGC 3 cut(s) 6, 742, 2553
Sau3AI GATC 7 cut(s) 109, 635, 873, 1196, 1321, 2383, 2416
Sau96I GGNCC 4 cut(s) 1543, 1624, 3027, 3124
ScaI AGTACT 1 cut(s) 2773
SchI GAGTC 6 cut(s) 227, 506, 2284, 2470, 2473, 2855
ScrFI CCNGG 5 cut(s) 978, 1548, 2283, 2779, 2795
SduI GDGCHC 7 cut(s) 56, 448, 579, 2501, 2526, 3309, 3331
SfaNI GCATC 8 cut(s) 52, 1078, 1439, 1456, 1498, 1567, 1695, 3266
SfcI CTRYAG 2 cut(s) 118, 2979
Sfr274I CTCGAG 1 cut(s) 2474
SfuI TTCGAA 1 cut(s) 1683
SinI GGWCC 3 cut(s) 1543, 3027, 3124
SlaI CTCGAG 1 cut(s) 2474
SmiMI CAYNNNNRTG 3 cut(s) 1938, 2172, 2693
SmlI CTYRAG 5 cut(s) 70, 1982, 1997, 2126, 2474
SmoI CTYRAG 5 cut(s) 70, 1982, 1997, 2126, 2474
SphI GCATGC 1 cut(s) 1100
SsiI CCGC 2 cut(s) 2145, 2327
SspMI CTAG 7 cut(s) 738, 1002, 1439, 1560, 1844, 2223, 3216
SstI GAGCTC 2 cut(s) 448, 2526
StyD4I CCNGG 5 cut(s) 976, 1546, 2281, 2777, 2793
StyI CCWWGG 2 cut(s) 246, 2923
TaiI ACGT 8 cut(s) 59, 304, 892, 1266, 1336, 1590, 2675, 3136
TaqI TCGA 7 cut(s) 876, 1683, 2475, 2508, 2718, 2748, 3094
TatI WGTACW 3 cut(s) 2191, 2561, 2771
TscAI CASTG 7 cut(s) 127, 2179, 2185, 2319, 2746, 2983, 3255
TseFI GTSAC 2 cut(s) 219, 3246
TseI GCWGC 3 cut(s) 5, 741, 2552
Tsp45I GTSAC 2 cut(s) 219, 3246
TspGWI ACGGA 1 cut(s) 217
TspRI CASTG 7 cut(s) 127, 2179, 2185, 2319, 2746, 2983, 3255
Vha464I CTTAAG 1 cut(s) 1997
VneI GTGCAC 2 cut(s) 52, 2497
VpaK11BI GGWCC 3 cut(s) 1543, 3027, 3124
VspI ATTAAT 1 cut(s) 2010
XagI CCTNNNNNAGG 1 cut(s) 1122
XapI RAATTY 3 cut(s) 728, 1774, 2584
XceI RCATGY 3 cut(s) 622, 1067, 1100
XcmI CCANNNNNNNNNTGG 5 cut(s) 1831, 2041, 2259, 2311, 3095
XhoI CTCGAG 1 cut(s) 2474
XmnI GAANNNNTTC 5 cut(s) 1722, 1764, 2136, 2514, 3173
XspI CTAG 7 cut(s) 738, 1002, 1439, 1560, 1844, 2223, 3216
ZrmI AGTACT 1 cut(s) 2773
Zsp2I ATGCAT 2 cut(s) 67, 1491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.