MD17G1275400.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000654
Physical Location & Seq
Forward (+)
33662307 .. 33666660
4354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1275400.v1.1.491

Sequence Viewer

Length: 2826 bp
ATGGCTGCTAAAAGTTCCCGAGATTCTTCTTCGTCCTCTTACCGGTGTTCCTATCATGCCTTCCTGAGTTTCAGGGGCCAGGACACGCGCAGGGGCTTTACTGATCACCTCTATAGAGCTTTGGAGATAGCAGGAATCCACACGTTTAGAGACGATGATGAAATCAAGAGAGGGGAGAATATTGAGTCGGAGTTAGAGAAGGCGATACAGGAGTCACAAGTATCGATAATTGTCTTCTCCAAGGACTACGCTTCCTCAAGGTGGTGTTTGAACGAACTTCTGAAGATCGTGGAACGTAGAAATACTGATCATAGACATGTGGTTCTGCCAGTTTTCTATGAGGTGGATCCATCCGATGTCAGGAAGCAGAGTGGTCCTTTTGCTGAAGCATTTGCCAGACATGAGGAACGATTCAGTACGGAGATGGACAAGGTGGAGCAGTGGAGAAGAGCTCTTGGAGATGTTGCATCTTTGGGAGGAATGGTTTTAGGAGATCGGTATGAGGGGCAGTTCATCCAAGAAATTGTTGAAGAGATTAGAAATAAAGTGGGTCACGCAGCTTTAGACGTCGCTCCCTTTGCAGTTGGAATGGATAATCGTGTGCAAGACCTAAACATGTGGCTACAAGATGGATCCAATGATGTTGGTGTAGCTGTCATCTGTGGGATGGGTGGAATTGGCAAGAGCACCATCGCGAAAGCCGCGTATAACCGCAACTTTGATAGATTTCAAGGTAGCAGCTTTCTTGCAGATATACGAGAATCTGCAGAACAACCCAATGGTTTTGCTTGCTTGCAAAGAAAACTTCTTTCAGATATCCAAAAGGGGAAAGCAAAGAAAGTATACAATATGGATGAAGGAACAATCAGGATCAAACAAGCCGTTGGCAATAAAAAAGTTCTTATTGTTCTTGATGATGTGAGCAACCGGGATCAACTCAATGCAATTCTTGGAATGCGAGAGTGGCTTCATCCAGGAAGTAAAATTATCATAACAACTAGACATGAGCATTTGTTAAATGCTCATAAAGTTTTTGAAAAGTTTATGGTTCAAGGACTGGATGAGGATGAATCACTAGAGCTTTTCTGTTGGCATGCCTTCAGACAATCCCATCCAGAAGAAGGTTACATGAAGCTTTCAAGACATGTGGTCCAGCACTGTGGAGGGCTTCCATTAGCTCTTCAAGTTCTGGGATCTTCTCTATCCGGAAAATGTGTAGAAGTGTGGCAAAGTGCATTGCAGAAGTTAGACGTGATCCCTAATGACAAAATTCAAAAAGTTCTGAGGATAAGTTTTGATTCTTTACAAGATGATCATGACAAAAATTTATTCCTTCACATAGCCAGTTTCTTCACAGGAAAGAGGATGGATTATACAAGTAGAATATTGGACAGCTTAGAGTTTTACACAAGGATTGGGATTGAAAATCTAGTTGACAGATGCCTAGTGAAAATTCAATGGAACAGGTTGGTCATGCATCAATTGGTGAGAAAAATGGCAATGGAAATTATTCGTGAAGAATCACCTGATGACCCTGGAAAACGTAGTAGAGTGTTGCAGAAAGATGCCTCTAATATTTTGAGAAAATTAAGTGGTACGGAAAATATTAAGGGCCTCATGCTCAACCTTGCTAGCAAAAGAACGTTTGTTCGAAGTGATAAAAAACGATGCCATGTTGAAGATTATGATGGAAATTGTTCAAGACGACGTCGGCTTGGTTTCTTCTCTTGGAAGCCAATTAGCTTTTCCTCCACAAACTCAGCTTCTGCATCAAATGAGATAGATTTCAAATCTGAGGCATTTAGAAGGATGCAGAATCTTGAAATTCTCATTCTCAACAACGTCAACATCAGGGGAAGCTACGAAGAATTTTCAAAAAATTTAGTATGTTTATCTTGGCGGGGATTCCACTTAAAATCAATACCAGAAAATTTTTGTTTGGAAAACCTGGTTGTTCTAGACATGCGAAATAGTAGCCTACAACATGTTTGGAATGGGACCAGGGTTATTCCGAGGCTGAAGATTCTTAATTTCAGTCATTCGCATGGCCTTAGGACAATCCCCGACTTCTCAGGACTCTATAACCTTGAGAGGTTGATTCTTAAACATTGCTTAAATTTGGTTGAGGTTCATGAGTCCATCGGACACCTAGAAAAACTTCTTGTTTTGAATCTAAAAGGCTGCAAAAATCTCATGAAGCTTCCAATATTGAGATCTATTCAGGATCTTATTCTTTCTGGTTGCTCAAAGCTTGAAATGAAGAAACTCAATCTGGTATCATCTAAATCATGGTATTCAGTCTGGTCGTGGGTGTCGCCAAGAAAAAATACTAAATTAAGCAGTTTCTCATTGGCAAGTTTACCACGTTCTCTGACAAGTTTAAGTCTGGATTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTCGTTAGAGTACTTGAATCTGGATGGTAATCCGATTACAAGCCTACCAGAAAGCATGAACTATCTTGTTAAGCTCCGGACTCTTAAAGGATCTGAATGCAGAAACCTCACAACACTTCCAGAGCTCCCACGTTCTTTGACGAGTTTAACGCTGGTTCGCTGCAATATATCAGAGATTCCAAGTTCCCTAACTATGCTGTCCGCGTTAAAGTACTTGAATCTGCATGGTAGTCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAGAGTATCTGGTTGCAGAAACCTCACAATGCTTCCAGAGCTCCCACGTAGTTTGGAACAATTGCATGATAGAGGTTGCGCATCATTGAAAAGAATAACAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

942

Amino Acids

107.08

Weight (kDa)

9.02

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 185 3.3e-50 TIR domain
TIR_2 PF13676 21 - 117 3.9e-17 TIR domain
NB-ARC PF00931 199 - 370 5.7e-20 NB-ARC domain
WHD_ROQ1 PF23282 438 - 508 1.3e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_8 PF13855 789 - 840 5.6e-07 Leucine rich repeat
LRR_14 PF23598 793 - 863 3.6e-06 Leucine-rich repeat region
LRR_8 PF13855 856 - 913 6.6e-07 Leucine rich repeat
LRR_14 PF23598 874 - 928 4.7e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 570, 1711
Acc16I TGCGCA 1 cut(s) 2800
AccI GTMKAC 1 cut(s) 843
AccII CGCG 4 cut(s) 88, 695, 704, 2639
AccIII TCCGGA 2 cut(s) 1205, 2511
AciI CCGC 4 cut(s) 702, 712, 1900, 2637
AclI AACGTT 1 cut(s) 1643
AcsI RAATTY 8 cut(s) 1269, 1324, 1452, 1824, 1868, 1879, 1930, 2116
AcuI CTGAAG 4 cut(s) 302, 405, 1084, 2039
AcyI GRCGYC 2 cut(s) 567, 1708
AfaI GTAC 4 cut(s) 418, 1597, 2447, 2648
AfiI CCNNNNNNNGG 4 cut(s) 42, 261, 360, 1121
AflIII ACRYGT 5 cut(s) 141, 316, 615, 1144, 1984
AgeI ACCGGT 1 cut(s) 42
AhdI GACNNNNNGTC 1 cut(s) 1148
AjiI CACGTC 1 cut(s) 1252
AjnI CCWGG 5 cut(s) 78, 973, 1534, 1947, 2000
AjuI GAANNNNNNNTTGG 2 cut(s) 1729, 1761
Alw21I GWGCWC 4 cut(s) 454, 689, 2562, 2763
Alw26I GTCTC 1 cut(s) 144
AlwNI CAGNNNCTG 1 cut(s) 1766
Ama87I CYCGRG 1 cut(s) 18
Aor13HI TCCGGA 2 cut(s) 1205, 2511
AoxI GGCC 3 cut(s) 76, 1612, 2047
ApeKI GCWGC 6 cut(s) 5, 557, 738, 2181, 2394, 2595
ApoI RAATTY 8 cut(s) 1269, 1324, 1452, 1824, 1868, 1879, 1930, 2116
AsiGI ACCGGT 1 cut(s) 42
Asp700I GAANNNNTTC 3 cut(s) 1509, 1696, 2157
AspLEI GCGC 2 cut(s) 90, 2801
AspS9I GGNCC 5 cut(s) 76, 374, 1150, 1612, 1998
AsuC2I CCSGG 1 cut(s) 929
AsuHPI GGTGA 3 cut(s) 98, 1498, 1515
AsuII TTCGAA 1 cut(s) 1651
AsuNHI GCTAGC 1 cut(s) 1631
AvaI CYCGRG 1 cut(s) 18
AvaII GGWCC 3 cut(s) 374, 1150, 1998
AxyI CCTNAGG 1 cut(s) 2051
BaeGI GKGCMC 1 cut(s) 2422
BaeI ACNNNNGTAYC 2 cut(s) 197, 230
BamHI GGATCC 2 cut(s) 346, 632
BanII GRGCYC 3 cut(s) 454, 2562, 2763
BbsI GAAGAC 1 cut(s) 226
Bbv12I GWGCWC 4 cut(s) 454, 689, 2562, 2763
BbvI GCAGC 5 cut(s) 569, 750, 2168, 2381, 2582
BceAI ACGGC 1 cut(s) 866
BciT130I CCWGG 5 cut(s) 80, 975, 1536, 1949, 2002
BclI TGATCA 3 cut(s) 103, 307, 1312
BcnI CCSGG 1 cut(s) 929
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 7 cut(s) 999, 1076, 1430, 1445, 1632, 1958, 2150
BfmI CTRYAG 2 cut(s) 112, 765
BglII AGATCT 1 cut(s) 2213
BisI GCNGC 7 cut(s) 6, 558, 702, 739, 2182, 2395, 2596
BlsI GCNGC 7 cut(s) 7, 559, 703, 740, 2183, 2396, 2597
BmcAI AGTACT 2 cut(s) 2447, 2648
Bme1390I CCNGG 6 cut(s) 80, 929, 975, 1536, 1949, 2002
Bme18I GGWCC 3 cut(s) 374, 1150, 1998
BmeRI GACNNNNNGTC 1 cut(s) 1148
BmeT110I CYCGRG 1 cut(s) 18
BmgBI CACGTC 1 cut(s) 1252
BmgT120I GGNCC 5 cut(s) 76, 374, 1150, 1612, 1998
BmiI GGNNCC 4 cut(s) 77, 348, 634, 1999
BmrFI CCNGG 6 cut(s) 80, 929, 975, 1536, 1949, 2002
BmsI GCATC 8 cut(s) 476, 1430, 1486, 1555, 1658, 1778, 1800, 2810
BmtI GCTAGC 1 cut(s) 1635
BpiI GAAGAC 1 cut(s) 226
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpmI CTGGAG 1 cut(s) 2696
Bpu14I TTCGAA 1 cut(s) 1651
BpuEI CTTGAG 2 cut(s) 241, 2108
BpuMI CCSGG 1 cut(s) 929
Bsa29I ATCGAT 1 cut(s) 224
BsaAI YACGTR 1 cut(s) 2768
BsaBI GATNNNNATC 1 cut(s) 2463
BsaHI GRCGYC 2 cut(s) 567, 1708
BsaJI CCNNGG 4 cut(s) 240, 1534, 2001, 2012
BsaWI WCCGGW 3 cut(s) 42, 1205, 2511
BsaXI ACNNNNNCTCC 2 cut(s) 468, 498
Bsc4I CCNNNNNNNGG 4 cut(s) 42, 261, 360, 1121
Bse118I RCCGGY 1 cut(s) 42
Bse1I ACTGG 3 cut(s) 329, 1062, 1344
Bse21I CCTNAGG 1 cut(s) 2051
Bse3DI GCAATG 3 cut(s) 1235, 1506, 2107
Bse8I GATNNNNATC 1 cut(s) 2463
BseAI TCCGGA 2 cut(s) 1205, 2511
BseBI CCWGG 5 cut(s) 80, 975, 1536, 1949, 2002
BseCI ATCGAT 1 cut(s) 224
BseDI CCNNGG 4 cut(s) 240, 1534, 2001, 2012
BseJI GATNNNNATC 1 cut(s) 2463
BseLI CCNNNNNNNGG 4 cut(s) 42, 261, 360, 1121
BseMI GCAATG 3 cut(s) 1235, 1506, 2107
BseMII CTCAG 5 cut(s) 56, 1274, 1773, 1785, 2085
BseNI ACTGG 3 cut(s) 329, 1062, 1344
BseSI GKGCMC 1 cut(s) 2422
BseXI GCAGC 5 cut(s) 569, 750, 2168, 2381, 2582
Bsh1236I CGCG 4 cut(s) 88, 695, 704, 2639
BshFI GGCC 3 cut(s) 78, 1614, 2049
BshTI ACCGGT 1 cut(s) 42
BshVI ATCGAT 1 cut(s) 224
BsiHKAI GWGCWC 4 cut(s) 454, 689, 2562, 2763
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 4 cut(s) 43, 928, 1206, 2512
BslFI GGGAC 1 cut(s) 2011
BslI CCNNNNNNNGG 4 cut(s) 42, 261, 360, 1121
BsmAI GTCTC 1 cut(s) 144
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 2011
BsmI GAATGC 2 cut(s) 960, 2537
BsnI GGCC 3 cut(s) 78, 1614, 2049
BsoBI CYCGRG 1 cut(s) 18
Bsp119I TTCGAA 1 cut(s) 1651
Bsp1286I GDGCHC 5 cut(s) 454, 689, 2422, 2562, 2763
Bsp13I TCCGGA 2 cut(s) 1205, 2511
Bsp68I TCGCGA 1 cut(s) 695
BspACI CCGC 4 cut(s) 702, 712, 1900, 2637
BspANI GGCC 3 cut(s) 78, 1614, 2049
BspCNI CTCAG 5 cut(s) 57, 1275, 1772, 1786, 2084
BspDI ATCGAT 1 cut(s) 224
BspEI TCCGGA 2 cut(s) 1205, 2511
BspFNI CGCG 4 cut(s) 88, 695, 704, 2639
BspHI TCATGA 3 cut(s) 1315, 2131, 2193
BspLI GGNNCC 4 cut(s) 77, 348, 634, 1999
BspMAI CTGCAG 1 cut(s) 769
BspOI GCTAGC 1 cut(s) 1635
BspQI GCTCTTC 2 cut(s) 442, 1185
BspT104I TTCGAA 1 cut(s) 1651
BsrDI GCAATG 3 cut(s) 1235, 1506, 2107
BsrFI RCCGGY 1 cut(s) 42
BsrI ACTGG 3 cut(s) 329, 1062, 1344
BssAI RCCGGY 1 cut(s) 42
BssECI CCNNGG 4 cut(s) 240, 1534, 2001, 2012
BssNAI GTATAC 1 cut(s) 844
BssNI GRCGYC 2 cut(s) 567, 1708
BssT1I CCWWGG 1 cut(s) 240
Bst1107I GTATAC 1 cut(s) 844
Bst2UI CCWGG 5 cut(s) 80, 975, 1536, 1949, 2002
Bst4CI ACNGT 1 cut(s) 1160
Bst6I CTCTTC 3 cut(s) 442, 525, 1185
BstACI GRCGYC 2 cut(s) 567, 1708
BstBAI YACGTR 1 cut(s) 2768
BstBI TTCGAA 1 cut(s) 1651
BstC8I GCNNGC 4 cut(s) 790, 794, 1095, 1633
BstDEI CTNAG 8 cut(s) 65, 1283, 1396, 1759, 1794, 2051, 2071, 2720
BstFNI CGCG 4 cut(s) 88, 695, 704, 2639
BstHHI GCGC 2 cut(s) 90, 2801
BstMAI GTCTC 1 cut(s) 144
BstMWI GCNNNNNNNGC 4 cut(s) 578, 701, 964, 2758
BstNI CCWGG 5 cut(s) 80, 975, 1536, 1949, 2002
BstNSI RCATGY 6 cut(s) 320, 619, 1097, 1148, 1966, 1988
BstSCI CCNGG 6 cut(s) 78, 927, 973, 1534, 1947, 2000
BstSFI CTRYAG 2 cut(s) 112, 765
BstSLI GKGCMC 1 cut(s) 2422
BstUI CGCG 4 cut(s) 88, 695, 704, 2639
BstV1I GCAGC 5 cut(s) 569, 750, 2168, 2381, 2582
BstV2I GAAGAC 1 cut(s) 226
BstX2I RGATCY 6 cut(s) 346, 632, 1193, 2213, 2224, 2525
BstXI CCANNNNNNTGG 1 cut(s) 1160
BstYI RGATCY 6 cut(s) 346, 632, 1193, 2213, 2224, 2525
BstZ17I GTATAC 1 cut(s) 844
Bsu15I ATCGAT 1 cut(s) 224
Bsu36I CCTNAGG 1 cut(s) 2051
BsuRI GGCC 3 cut(s) 78, 1614, 2049
BsuTUI ATCGAT 1 cut(s) 224
BtgZI GCGATG 1 cut(s) 676
BtrI CACGTC 1 cut(s) 1252
BtsI GCAGTG 1 cut(s) 446
BtsIMutI CAGTG 2 cut(s) 446, 1156
BtuMI TCGCGA 1 cut(s) 695
Cac8I GCNNGC 4 cut(s) 790, 794, 1095, 1633
CaiI CAGNNNCTG 1 cut(s) 1766
CciI TCATGA 3 cut(s) 1315, 2131, 2193
CfoI GCGC 2 cut(s) 90, 2801
Cfr10I RCCGGY 1 cut(s) 42
Cfr13I GGNCC 5 cut(s) 76, 374, 1150, 1612, 1998
ClaI ATCGAT 1 cut(s) 224
CsiI ACCWGGT 1 cut(s) 1947
Csp6I GTAC 4 cut(s) 417, 1596, 2446, 2647
CspAI ACCGGT 1 cut(s) 42
CspCI CAANNNNNGTGG 6 cut(s) 1128, 1163, 2553, 2588, 2754, 2789
CviQI GTAC 4 cut(s) 417, 1596, 2446, 2647
DdeI CTNAG 8 cut(s) 65, 1283, 1396, 1759, 1794, 2051, 2071, 2720
DriI GACNNNNNGTC 1 cut(s) 1148
Eam1104I CTCTTC 3 cut(s) 442, 525, 1185
Eam1105I GACNNNNNGTC 1 cut(s) 1148
EarI CTCTTC 3 cut(s) 442, 525, 1185
Ecl136II GAGCTC 3 cut(s) 452, 2560, 2761
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 3 cut(s) 454, 2562, 2763
Eco32I GATATC 1 cut(s) 817
Eco47I GGWCC 3 cut(s) 374, 1150, 1998
Eco53kI GAGCTC 3 cut(s) 452, 2560, 2761
Eco57I CTGAAG 4 cut(s) 302, 405, 1084, 2039
Eco81I CCTNAGG 1 cut(s) 2051
Eco88I CYCGRG 1 cut(s) 18
EcoICRI GAGCTC 3 cut(s) 452, 2560, 2761
EcoO109I RGGNCCY 1 cut(s) 1612
EcoRII CCWGG 5 cut(s) 78, 973, 1534, 1947, 2000
EcoRV GATATC 1 cut(s) 817
EcoT14I CCWWGG 1 cut(s) 240
EcoT22I ATGCAT 1 cut(s) 1479
EcoT38I GRGCYC 3 cut(s) 454, 2562, 2763
ErhI CCWWGG 1 cut(s) 240
Esp3I CGTCTC 1 cut(s) 144
FaqI GGGAC 1 cut(s) 2011
FauI CCCGC 1 cut(s) 1893
FbaI TGATCA 3 cut(s) 103, 307, 1312
FblI GTMKAC 1 cut(s) 843
Fnu4HI GCNGC 7 cut(s) 6, 558, 702, 739, 2182, 2395, 2596
FriOI GRGCYC 3 cut(s) 454, 2562, 2763
Fsp4HI GCNGC 7 cut(s) 6, 558, 702, 739, 2182, 2395, 2596
FspBI CTAG 7 cut(s) 999, 1076, 1430, 1445, 1632, 1958, 2150
FspI TGCGCA 1 cut(s) 2800
GlaI GCGC 2 cut(s) 89, 2800
GluI GCNGC 7 cut(s) 6, 558, 702, 739, 2182, 2395, 2596
GsuI CTGGAG 1 cut(s) 2696
HaeIII GGCC 3 cut(s) 78, 1614, 2049
HapII CCGG 4 cut(s) 43, 928, 1206, 2512
HhaI GCGC 2 cut(s) 90, 2801
Hin1I GRCGYC 2 cut(s) 567, 1708
Hin6I GCGC 2 cut(s) 88, 2799
HinP1I GCGC 2 cut(s) 88, 2799
HincII GTYRAC 2 cut(s) 1435, 1846
HindII GTYRAC 2 cut(s) 1435, 1846
HindIII AAGCTT 3 cut(s) 1133, 2198, 2249
HpaII CCGG 4 cut(s) 43, 928, 1206, 2512
HphI GGTGA 3 cut(s) 98, 1498, 1515
Hpy166II GTNNAC 4 cut(s) 844, 1435, 1846, 2360
Hpy8I GTNNAC 4 cut(s) 844, 1435, 1846, 2360
Hpy99I CGWCG 3 cut(s) 572, 1710, 1713
HpyAV CCTTC 7 cut(s) 70, 193, 851, 1108, 1115, 1343, 1800
HpyCH4III ACNGT 1 cut(s) 1160
HpyF10VI GCNNNNNNNGC 4 cut(s) 578, 701, 964, 2758
HpyF3I CTNAG 8 cut(s) 65, 1283, 1396, 1759, 1794, 2051, 2071, 2720
Hsp92I GRCGYC 2 cut(s) 567, 1708
HspAI GCGC 2 cut(s) 88, 2799
Kpn2I TCCGGA 2 cut(s) 1205, 2511
Ksp22I TGATCA 3 cut(s) 103, 307, 1312
LguI GCTCTTC 2 cut(s) 442, 1185
LmnI GCTCC 6 cut(s) 436, 577, 2514, 2565, 2715, 2766
Lsp1109I GCAGC 5 cut(s) 569, 750, 2168, 2381, 2582
LweI GCATC 8 cut(s) 476, 1430, 1486, 1555, 1658, 1778, 1800, 2810
MabI ACCWGGT 1 cut(s) 1947
MaeI CTAG 7 cut(s) 999, 1076, 1430, 1445, 1632, 1958, 2150
MaeIII GTNAC 3 cut(s) 213, 551, 1124
MfeI CAATTG 2 cut(s) 1481, 2780
MflI RGATCY 6 cut(s) 346, 632, 1193, 2213, 2224, 2525
MhlI GDGCHC 5 cut(s) 454, 689, 2422, 2562, 2763
MlyI GAGTC 6 cut(s) 194, 221, 2070, 2144, 2509, 2710
MmeI TCCRAC 2 cut(s) 168, 565
Mph1103I ATGCAT 1 cut(s) 1479
MroI TCCGGA 2 cut(s) 1205, 2511
MroXI GAANNNNTTC 3 cut(s) 1509, 1696, 2157
MslI CAYNNNNRTG 2 cut(s) 315, 2043
MspI CCGG 4 cut(s) 43, 928, 1206, 2512
MspR9I CCNGG 6 cut(s) 80, 929, 975, 1536, 1949, 2002
MunI CAATTG 2 cut(s) 1481, 2780
Mva1269I GAATGC 2 cut(s) 960, 2537
MvaI CCWGG 5 cut(s) 80, 975, 1536, 1949, 2002
MvnI CGCG 4 cut(s) 88, 695, 704, 2639
MwoI GCNNNNNNNGC 4 cut(s) 578, 701, 964, 2758
NciI CCSGG 1 cut(s) 929
NheI GCTAGC 1 cut(s) 1631
NlaIV GGNNCC 4 cut(s) 77, 348, 634, 1999
NmuCI GTSAC 2 cut(s) 213, 551
NruI TCGCGA 1 cut(s) 695
NsbI TGCGCA 1 cut(s) 2800
NsiI ATGCAT 1 cut(s) 1479
NspI RCATGY 6 cut(s) 320, 619, 1097, 1148, 1966, 1988
NspV TTCGAA 1 cut(s) 1651
PaeI GCATGC 1 cut(s) 1097
PagI TCATGA 3 cut(s) 1315, 2131, 2193
PciI ACATGT 4 cut(s) 316, 615, 1144, 1984
PciSI GCTCTTC 2 cut(s) 442, 1185
PctI GAATGC 2 cut(s) 960, 2537
PdmI GAANNNNTTC 3 cut(s) 1509, 1696, 2157
PflFI GACNNNGTC 1 cut(s) 1707
PfoI TCCNGGA 1 cut(s) 973
PinAI ACCGGT 1 cut(s) 42
PkrI GCNGC 7 cut(s) 7, 559, 703, 740, 2183, 2396, 2597
PleI GAGTC 6 cut(s) 193, 220, 2070, 2143, 2509, 2710
PpsI GAGTC 6 cut(s) 193, 220, 2070, 2143, 2509, 2710
Ppu21I YACGTR 1 cut(s) 2768
PscI ACATGT 4 cut(s) 316, 615, 1144, 1984
Psp124BI GAGCTC 3 cut(s) 454, 2562, 2763
Psp1406I AACGTT 1 cut(s) 1643
Psp6I CCWGG 5 cut(s) 78, 973, 1534, 1947, 2000
PspGI CCWGG 5 cut(s) 78, 973, 1534, 1947, 2000
PspN4I GGNNCC 4 cut(s) 77, 348, 634, 1999
PspPI GGNCC 5 cut(s) 76, 374, 1150, 1612, 1998
PstI CTGCAG 1 cut(s) 769
PstNI CAGNNNCTG 1 cut(s) 1766
PsuI RGATCY 6 cut(s) 346, 632, 1193, 2213, 2224, 2525
PsyI GACNNNGTC 1 cut(s) 1707
RruI TCGCGA 1 cut(s) 695
RsaI GTAC 4 cut(s) 418, 1597, 2447, 2648
RsaNI GTAC 4 cut(s) 417, 1596, 2446, 2647
RseI CAYNNNNRTG 2 cut(s) 315, 2043
SacI GAGCTC 3 cut(s) 454, 2562, 2763
SapI GCTCTTC 2 cut(s) 442, 1185
SatI GCNGC 7 cut(s) 6, 558, 702, 739, 2182, 2395, 2596
Sau96I GGNCC 5 cut(s) 76, 374, 1150, 1612, 1998
ScaI AGTACT 2 cut(s) 2447, 2648
SchI GAGTC 6 cut(s) 194, 221, 2070, 2144, 2509, 2710
ScrFI CCNGG 6 cut(s) 80, 929, 975, 1536, 1949, 2002
SduI GDGCHC 5 cut(s) 454, 689, 2422, 2562, 2763
SexAI ACCWGGT 1 cut(s) 1947
SfaNI GCATC 8 cut(s) 476, 1430, 1486, 1555, 1658, 1778, 1800, 2810
SfcI CTRYAG 2 cut(s) 112, 765
SfuI TTCGAA 1 cut(s) 1651
SinI GGWCC 3 cut(s) 374, 1150, 1998
SmiMI CAYNNNNRTG 2 cut(s) 315, 2043
SmlI CTYRAG 2 cut(s) 256, 2087
SmoI CTYRAG 2 cut(s) 256, 2087
SphI GCATGC 1 cut(s) 1097
SsiI CCGC 4 cut(s) 702, 712, 1900, 2637
SspI AATATT 5 cut(s) 181, 1386, 1576, 1606, 2208
SspMI CTAG 7 cut(s) 999, 1076, 1430, 1445, 1632, 1958, 2150
SstI GAGCTC 3 cut(s) 454, 2562, 2763
StyD4I CCNGG 6 cut(s) 78, 927, 973, 1534, 1947, 2000
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 1160
TaqI TCGA 2 cut(s) 224, 1651
TatI WGTACW 2 cut(s) 2445, 2646
TauI GCSGC 1 cut(s) 704
TscAI CASTG 2 cut(s) 446, 1163
TseFI GTSAC 2 cut(s) 213, 551
TseI GCWGC 6 cut(s) 5, 557, 738, 2181, 2394, 2595
Tsp45I GTSAC 2 cut(s) 213, 551
TspGWI ACGGA 2 cut(s) 434, 1613
TspRI CASTG 2 cut(s) 446, 1163
Tth111I GACNNNGTC 1 cut(s) 1707
VpaK11BI GGWCC 3 cut(s) 374, 1150, 1998
XapI RAATTY 8 cut(s) 1269, 1324, 1452, 1824, 1868, 1879, 1930, 2116
XbaI TCTAGA 1 cut(s) 1957
XceI RCATGY 6 cut(s) 320, 619, 1097, 1148, 1966, 1988
XmiI GTMKAC 1 cut(s) 843
XmnI GAANNNNTTC 3 cut(s) 1509, 1696, 2157
XspI CTAG 7 cut(s) 999, 1076, 1430, 1445, 1632, 1958, 2150
ZraI GACGTC 2 cut(s) 568, 1709
ZrmI AGTACT 2 cut(s) 2447, 2648
Zsp2I ATGCAT 1 cut(s) 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.