Prupe.3G010100_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000654
Physical Location & Seq
Forward (+)
646107 .. 650051
3945 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G010100.1

Sequence Viewer

Length: 3339 bp
ATGGCTGCTACCAGCACGCAGCAGGATCCGTCCTCTCACCGGTGCACTTATGATGCATTCTTGAGCTTCAGGGGCACAGATACACGCAAGGGATTTACTGATCACCTTTACAGGGCTTTGGAGGTGGCAGGGATCCACACTTTTAGAGATGATGACGAAATCGAAAGAGGAGCAAATATTTCAGCAGAGCTTCAAAAAGCAATACAAGAGTCTCGAGTATCCATCATTGTCTTCTCCAAGGACTACGCTTCCTCGAGATGGTGCCTGGATGAACTAGTGACGATCATGGACCGTAGAGAAACTAATGAGCACATGGTTATGCCGATTTTCTATGATGTGGATCCATCCCATGTCAGGAACCAGACAGGAATTTTTGAACAAGCTTTTGCCAGACACCAACAGCGCTTCAACAAGGAGATGGACAAGGTGGAGAAGTGGAGAAAAGCTCTCAGAGATGTTGCAGATTTGGGAGGGATGGTTTTAGGAGATCGGTACGAGTCGCAGTTTGTCCAAGATATAGTTGAAATTATTGGAAATAAACTGGATCACACATGGAATAGGAGATTAAGAGTCGATCCCTATGTAGTTGGAATGGATTATCGTGTGAGAGGCCTAAACATGTGGTTAGAAGATGGATCAAGTGATGTTGGAGTAGCTGTAGTCTATGGGATGGGTGGAATTGGCAAGACCACCATTGCCAAGACTGCTTATAACCAGAACTACAATAAATTTCAAGGTAGCAGCTTTCTTGCAGATATTAGAGCAACTTCAAAACTTCCCAATGGTTTTGTACACTTGCAAAGGAACCTTCTTTCAGATCTCCAAAAGGGGAAAGCAAAGAAAATATACAGCTTGGATGAAGGAATAACAAAGATCAAACGGGCGATACGTTGCAAAAGAGTTCTTATTGCTCTTGATGATGTGGACAACTTGGAACAATTCAATGCAATTCTTGGAATGCGAGAATGGCTTCATCCAGGAAGTAAAATAATCATAACAACTAGACATGAACATTTGTTAAAGGCTCATGAGAATTATGCAATATTGTTTAAGGTGGAAGGGTTGCATGAGTATGAATCACTTGAGCTTTTCAGTTGGCATGCCTTTAGACAACCCCATCCTAGCGAAGGTTACATGGATCTTTCAAGACCTGTAGTGCAACACTGTGGAGGGGTTCCATTAGCTCTTCAAGTTCTAGGATCTTCTCTATTTGGTAAAGCTGCAGATGTATGGAAAAATGCATTACAGAACTTAGATGTAATTACTGAAGGAAAAATTCAAAAGATTCTCAGAATAAGCTTTGATTCTCTGCAAGATCATGACAAACGTCTGTTCCTCCATATAGCCTGTTTCTTCGTAGGAAAAGACAATGATTTTTCAACTACAGTCCTTGATGAATGTGAGTTTGCCACAAACATTGGAATGCAAAATCTGGTTGATAGATGTTTGCTGATAATTGATGGATTCAACAAGTTAACCATGCACCAATTACTTCAAGACATGGGAAGGGGAATTATTCGCGAAGAATCGCCTGAGGATCCTGGAAAACGTACTAGAGTGTGGAATAAAGATGCCTCTAATGTTTTGAGAAAATTAACTGGTACAGAAACTATTAAGGGCCTCGTGCTCAACATTCCTATGTTAATAAAAGATGAGTCTTCTAAGATAATATTCAGTGGAAGTAACAGAAAAAGATTCCATGTTGAAGATTATGATGGAAACTGTTCAAGCAGCCGACGTCGGCTTGGTTTCTTCTCCTGGCAGTCAATTACAAACTCATTTCCTGTATCAAATGAGATAGGTTTCAAAACAGAGGCATTTAGAAGGATGCACAATCTTGAACTCCTGCTGCTTGATAATGTAAAAATCAGTGGAGGCTATGAAGATTTCCCAAAAAATTTAATATGGTTGTCTTGGCGAGGATTCGCTTTAAAGTCATTACCAACCAATTTTTACTTGGAAAATCTAATTGTTCTTGACTTGCGGAACAGCAGCCTCCAACATGTTTGGAAGGGAACTAGGTTTCTTCTAAGACTGAAAATCTTAAATCTCAGTCATTCACATGGCCTTGTGACAACCCCTGACTTGTCAGGACTCCCTAACCTCGAGAGATTAATTCTTAAAGATTGCATAAATTTGAAAGAGGTGGATGAATCCATTGGAGATTTGGAGAAACTTGTTTTCTTGAATCTAAAAGATTGCAAAAATCTCATGAAGCTTCCAATAAGAATTAGTATGCTGCGATCTCTCCAGAAACTTATTCTCTCTGGTTGCCCAAATCTTGTGCTGCCTGCCAGTATGATTGTTAAAAATCAGTCAGACTCTGTACCTAGTGACATGAAGCAACTCAGTCTGTTATCTGCAGTTAAATCGTGGCAATCAATCCGATCATGGGTATTGCCAAGAAAAAATCTCCAACTTACCAGTGCAAGTTTGCCACAATTTTTAAAAAGCTTAAGTATGGCATACTGCAATCTGTCAGAAATTCCCGATGGTCTTAGTAGCCTATCCTCATTGAAGCATTTGAATCTAAGTGGAAACCCATTTTTGAGCCTAAACATGAATGGTCTTAGTAAGCTCCAGAGTCTTTTGTTGGATGGTTGCACAAACCTCGAAATGCTTGCCGAGCTCCCACCGAGTGTAGAGAGATTGCAAGCATGGGAATGTACTTCATTGAAAAGAGTACAAACTTACTTACCCAACAAGTTATCAATGGGACATGAGGTTTTGAGATGCGAGAATTTAGTTGAATTTCAAAACGTGTTCAAAACAAGACCGTTGAGAAGCTTCGACATAGAAATGATCAAAGATATCGGTCTGTTCAATTTGGAATCCATAGGAAGCACTGAAGTTGAAATGATCAACTACTTGACACGTACAACGAGGAAGGGTCCTCTCCAGGGACTGGATGAATGTGGTATATTTAGCATATTCCTTCCTGGGAGCGAGGTTCCAGATTGGTTCTGCTACAAGTCGAGCATGGGTAACTCTGAGCTGTCTATAACTATACCTCCACATCTTAATTTAAAGATCCGAGGCCTAAATGCATGTGTTGTTTATGCACAAGGTAAGGTTCTTGTTGAGGATTTGAAATGTTACTCGGTCGTTCCCTTTCTTCGCATCAGCAATGAGACCAAGGGTTTTAAGTGGACCTATTTGCCAGTCACAATAGGGTTTCCAAAAGAGAAGGAAGACATGTTATGGCTTAGTCATTGGCGATTCACAAACGATGAATTGGAGGGTGGGGAAGAAATACGTGTTTCAGTTAGAGATGAATCCGATAGTTTCTGGACAAAGGAATTTGGAATCCAGCTTGTGTACGAGCAGAATAATAATGAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1113

Amino Acids

127.0

Weight (kDa)

7.56

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 711
AatII GACGTC 1 cut(s) 1741
AccB1I GGYRCC 1 cut(s) 261
AccB7I CCANNNNNTGG 1 cut(s) 2905
AccII CGCG 1 cut(s) 1521
AciI CCGC 1 cut(s) 1984
AcsI RAATTY 9 cut(s) 369, 728, 1275, 1897, 2134, 2484, 2740, 2750, 3299
AcuI CTGAAG 3 cut(s) 52, 1287, 2868
AcyI GRCGYC 1 cut(s) 1738
AdeI CACNNNGTG 1 cut(s) 2639
AfaI GTAC 9 cut(s) 494, 792, 1552, 1603, 2328, 2668, 2685, 2878, 3320
AfeI AGCGCT 1 cut(s) 404
AfiI CCNNNNNNNGG 8 cut(s) 39, 112, 258, 354, 1127, 2392, 2900, 2905
AflII CTTAAG 1 cut(s) 2455
AflIII ACRYGT 6 cut(s) 618, 2002, 2760, 2873, 3195, 3256
AgeI ACCGGT 1 cut(s) 39
AhlI ACTAGT 1 cut(s) 274
AjnI CCWGG 6 cut(s) 264, 976, 1540, 1757, 2898, 2938
Alw21I GWGCWC 4 cut(s) 47, 312, 1629, 2631
Alw26I GTCTC 2 cut(s) 216, 3125
Alw44I GTGCAC 1 cut(s) 43
AlwNI CAGNNNCTG 2 cut(s) 2324, 2905
Ama87I CYCGRG 3 cut(s) 213, 253, 2105
Aor51HI AGCGCT 1 cut(s) 404
AoxI GGCC 4 cut(s) 610, 1618, 2065, 3037
ApaLI GTGCAC 1 cut(s) 43
ApeKI GCWGC 9 cut(s) 5, 19, 741, 1220, 1731, 1849, 1992, 2239, 2287
ApoI RAATTY 9 cut(s) 369, 728, 1275, 1897, 2134, 2484, 2740, 2750, 3299
AseI ATTAAT 1 cut(s) 2114
AsiGI ACCGGT 1 cut(s) 39
Asp700I GAANNNNTTC 2 cut(s) 969, 1723
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 4 cut(s) 289, 1618, 2891, 3150
AsuHPI GGTGA 2 cut(s) 29, 95
AvaI CYCGRG 3 cut(s) 213, 253, 2105
AvaII GGWCC 3 cut(s) 289, 2891, 3150
AxyI CCTNAGG 1 cut(s) 1533
BaeGI GKGCMC 2 cut(s) 47, 77
BamHI GGATCC 4 cut(s) 25, 132, 340, 1537
BanI GGYRCC 1 cut(s) 261
BanII GRGCYC 1 cut(s) 2631
BauI CACGAG 1 cut(s) 1622
BbsI GAAGAC 3 cut(s) 223, 1650, 3198
Bbv12I GWGCWC 4 cut(s) 47, 312, 1629, 2631
BbvI GCAGC 8 cut(s) 31, 753, 1207, 1743, 1836, 2004, 2226, 2274
BcgI CGANNNNNNTGC 6 cut(s) 2352, 2386, 2593, 2603, 2627, 2637
BciT130I CCWGG 6 cut(s) 266, 978, 1542, 1759, 2900, 2940
BciVI GTATCC 1 cut(s) 229
BclI TGATCA 3 cut(s) 100, 2802, 2859
BcoDI GTCTC 2 cut(s) 216, 3125
BcuI ACTAGT 1 cut(s) 274
BfaI CTAG 7 cut(s) 275, 1002, 1122, 1196, 1554, 2019, 2331
BfmI CTRYAG 5 cut(s) 657, 1152, 1221, 1383, 2361
BfoI RGCGCY 1 cut(s) 406
BfrI CTTAAG 1 cut(s) 2455
BfuI GTATCC 1 cut(s) 229
BglII AGATCT 1 cut(s) 817
BisI GCNGC 9 cut(s) 6, 20, 742, 1221, 1732, 1850, 1993, 2240, 2288
BlsI GCNGC 9 cut(s) 7, 21, 743, 1222, 1733, 1851, 1994, 2241, 2289
Bme1390I CCNGG 6 cut(s) 266, 978, 1542, 1759, 2900, 2940
Bme18I GGWCC 3 cut(s) 289, 2891, 3150
BmeT110I CYCGRG 3 cut(s) 213, 253, 2105
BmgT120I GGNCC 4 cut(s) 289, 1618, 2891, 3150
BmrFI CCNGG 6 cut(s) 266, 978, 1542, 1759, 2900, 2940
BmsI GCATC 5 cut(s) 43, 1561, 1818, 2723, 3129
BoxI GACNNNNGTC 1 cut(s) 1326
BpiI GAAGAC 3 cut(s) 223, 1650, 3198
BplI GAGNNNNNCTC 2 cut(s) 430, 462
BpmI CTGGAG 3 cut(s) 2234, 2564, 2882
BpuEI CTTGAG 2 cut(s) 82, 1103
BsaAI YACGTR 2 cut(s) 2876, 3257
BsaBI GATNNNNATC 1 cut(s) 339
BsaHI GRCGYC 1 cut(s) 1738
BsaI GGTCTC 1 cut(s) 3125
BsaJI CCNNGG 5 cut(s) 237, 2899, 2939, 3034, 3135
BsaWI WCCGGW 1 cut(s) 39
BsaXI ACNNNNNCTCC 6 cut(s) 462, 492, 642, 672, 2995, 3025
Bsc4I CCNNNNNNNGG 8 cut(s) 39, 112, 258, 354, 1127, 2392, 2900, 2905
Bse118I RCCGGY 1 cut(s) 39
Bse1I ACTGG 6 cut(s) 546, 1603, 2295, 2424, 2910, 3161
Bse21I CCTNAGG 1 cut(s) 1533
Bse3DI GCAATG 2 cut(s) 693, 3133
Bse8I GATNNNNATC 1 cut(s) 339
BseBI CCWGG 6 cut(s) 266, 978, 1542, 1759, 2900, 2940
BseDI CCNNGG 5 cut(s) 237, 2899, 2939, 3034, 3135
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 8 cut(s) 39, 112, 258, 354, 1127, 2392, 2900, 2905
BseMI GCAATG 2 cut(s) 693, 3133
BseMII CTCAG 6 cut(s) 463, 1303, 1524, 2065, 2362, 2982
BseNI ACTGG 6 cut(s) 546, 1603, 2295, 2424, 2910, 3161
BseRI GAGGAG 1 cut(s) 183
BseSI GKGCMC 2 cut(s) 47, 77
BseXI GCAGC 8 cut(s) 31, 753, 1207, 1743, 1836, 2004, 2226, 2274
Bsh1236I CGCG 1 cut(s) 1521
Bsh1285I CGRYCG 1 cut(s) 3105
BshFI GGCC 4 cut(s) 612, 1620, 2067, 3039
BshNI GGYRCC 1 cut(s) 261
BshTI ACCGGT 1 cut(s) 39
BsiEI CGRYCG 1 cut(s) 3105
BsiHKAI GWGCWC 4 cut(s) 47, 312, 1629, 2631
BsiHKCI CYCGRG 3 cut(s) 213, 253, 2105
BsiSI CCGG 1 cut(s) 40
BslFI GGGAC 2 cut(s) 2730, 2916
BslI CCNNNNNNNGG 8 cut(s) 39, 112, 258, 354, 1127, 2392, 2900, 2905
BsmAI GTCTC 2 cut(s) 216, 3125
BsmFI GGGAC 2 cut(s) 2730, 2916
BsmI GAATGC 3 cut(s) 56, 963, 1428
BsnI GGCC 4 cut(s) 612, 1620, 2067, 3039
Bso31I GGTCTC 1 cut(s) 3125
BsoBI CYCGRG 3 cut(s) 213, 253, 2105
Bsp1286I GDGCHC 5 cut(s) 47, 77, 312, 1629, 2631
Bsp1407I TGTACA 1 cut(s) 790
Bsp68I TCGCGA 1 cut(s) 1521
BspACI CCGC 1 cut(s) 1984
BspANI GGCC 4 cut(s) 612, 1620, 2067, 3039
BspCNI CTCAG 6 cut(s) 462, 1302, 1525, 2064, 2361, 2983
BspFNI CGCG 1 cut(s) 1521
BspHI TCATGA 3 cut(s) 1027, 1318, 2211
BspMAI CTGCAG 2 cut(s) 1225, 2365
BspQI GCTCTTC 1 cut(s) 1191
BspT107I GGYRCC 1 cut(s) 261
BspTI CTTAAG 1 cut(s) 2455
BspTNI GGTCTC 1 cut(s) 3125
BsrDI GCAATG 2 cut(s) 693, 3133
BsrFI RCCGGY 1 cut(s) 39
BsrGI TGTACA 1 cut(s) 790
BsrI ACTGG 6 cut(s) 546, 1603, 2295, 2424, 2910, 3161
BssAI RCCGGY 1 cut(s) 39
BssECI CCNNGG 5 cut(s) 237, 2899, 2939, 3034, 3135
BssNI GRCGYC 1 cut(s) 1738
BssSI CACGAG 1 cut(s) 1622
BssT1I CCWWGG 2 cut(s) 237, 3135
Bst2BI CACGAG 1 cut(s) 1622
Bst2UI CCWGG 6 cut(s) 266, 978, 1542, 1759, 2900, 2940
Bst4CI ACNGT 5 cut(s) 293, 1166, 1387, 1724, 2778
Bst6I CTCTTC 1 cut(s) 1191
BstACI GRCGYC 1 cut(s) 1738
BstAFI CTTAAG 1 cut(s) 2455
BstAUI TGTACA 1 cut(s) 790
BstBAI YACGTR 2 cut(s) 2876, 3257
BstC8I GCNNGC 5 cut(s) 17, 1101, 2292, 2622, 2655
BstENI CCTNNNNNAGG 1 cut(s) 1125
BstFNI CGCG 1 cut(s) 1521
BstH2I RGCGCY 1 cut(s) 406
BstHHI GCGC 1 cut(s) 405
BstMAI GTCTC 2 cut(s) 216, 3125
BstMCI CGRYCG 1 cut(s) 3105
BstMWI GCNNNNNNNGC 4 cut(s) 72, 704, 967, 2626
BstNI CCWGG 6 cut(s) 266, 978, 1542, 1759, 2900, 2940
BstNSI RCATGY 5 cut(s) 622, 1103, 2006, 3051, 3199
BstPAI GACNNNNGTC 1 cut(s) 1326
BstSCI CCNGG 6 cut(s) 264, 976, 1540, 1757, 2898, 2938
BstSFI CTRYAG 5 cut(s) 657, 1152, 1221, 1383, 2361
BstSLI GKGCMC 2 cut(s) 47, 77
BstUI CGCG 1 cut(s) 1521
BstV1I GCAGC 8 cut(s) 31, 753, 1207, 1743, 1836, 2004, 2226, 2274
BstV2I GAAGAC 3 cut(s) 223, 1650, 3198
BstX2I RGATCY 8 cut(s) 25, 132, 340, 817, 1138, 1199, 1537, 3030
BstYI RGATCY 8 cut(s) 25, 132, 340, 817, 1138, 1199, 1537, 3030
Bsu36I CCTNAGG 1 cut(s) 1533
BsuI GTATCC 1 cut(s) 229
BsuRI GGCC 4 cut(s) 612, 1620, 2067, 3039
BtsIMutI CAGTG 5 cut(s) 1162, 1681, 1876, 2431, 2844
BtuMI TCGCGA 1 cut(s) 1521
Cac8I GCNNGC 5 cut(s) 17, 1101, 2292, 2622, 2655
CaiI CAGNNNCTG 2 cut(s) 2324, 2905
CciI TCATGA 3 cut(s) 1027, 1318, 2211
CfoI GCGC 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 39
Cfr13I GGNCC 4 cut(s) 289, 1618, 2891, 3150
Csp6I GTAC 9 cut(s) 493, 791, 1551, 1602, 2327, 2667, 2684, 2877, 3319
CspAI ACCGGT 1 cut(s) 39
CspCI CAANNNNNGTGG 2 cut(s) 1399, 1434
CviQI GTAC 9 cut(s) 493, 791, 1551, 1602, 2327, 2667, 2684, 2877, 3319
DraI TTTAAA 3 cut(s) 1932, 2448, 3027
DraIII CACNNNGTG 1 cut(s) 2639
Eam1104I CTCTTC 1 cut(s) 1191
EarI CTCTTC 1 cut(s) 1191
Ecl136II GAGCTC 1 cut(s) 2629
Eco130I CCWWGG 2 cut(s) 237, 3135
Eco147I AGGCCT 2 cut(s) 612, 3039
Eco24I GRGCYC 1 cut(s) 2631
Eco31I GGTCTC 1 cut(s) 3125
Eco32I GATATC 1 cut(s) 2812
Eco47I GGWCC 3 cut(s) 289, 2891, 3150
Eco47III AGCGCT 1 cut(s) 404
Eco53kI GAGCTC 1 cut(s) 2629
Eco57I CTGAAG 3 cut(s) 52, 1287, 2868
Eco81I CCTNAGG 1 cut(s) 1533
Eco88I CYCGRG 3 cut(s) 213, 253, 2105
EcoICRI GAGCTC 1 cut(s) 2629
EcoNI CCTNNNNNAGG 1 cut(s) 1125
EcoO109I RGGNCCY 2 cut(s) 1618, 2891
EcoRII CCWGG 6 cut(s) 264, 976, 1540, 1757, 2898, 2938
EcoRV GATATC 1 cut(s) 2812
EcoT14I CCWWGG 2 cut(s) 237, 3135
EcoT22I ATGCAT 3 cut(s) 58, 1243, 3049
EcoT38I GRGCYC 1 cut(s) 2631
ErhI CCWWGG 2 cut(s) 237, 3135
FaqI GGGAC 2 cut(s) 2730, 2916
FbaI TGATCA 3 cut(s) 100, 2802, 2859
Fnu4HI GCNGC 9 cut(s) 6, 20, 742, 1221, 1732, 1850, 1993, 2240, 2288
FriOI GRGCYC 1 cut(s) 2631
Fsp4HI GCNGC 9 cut(s) 6, 20, 742, 1221, 1732, 1850, 1993, 2240, 2288
FspBI CTAG 7 cut(s) 275, 1002, 1122, 1196, 1554, 2019, 2331
GlaI GCGC 1 cut(s) 404
GluI GCNGC 9 cut(s) 6, 20, 742, 1221, 1732, 1850, 1993, 2240, 2288
GsuI CTGGAG 3 cut(s) 2234, 2564, 2882
HaeII RGCGCY 1 cut(s) 406
HaeIII GGCC 4 cut(s) 612, 1620, 2067, 3039
HapII CCGG 1 cut(s) 40
HhaI GCGC 1 cut(s) 405
Hin1I GRCGYC 1 cut(s) 1738
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HincII GTYRAC 1 cut(s) 1476
HindII GTYRAC 1 cut(s) 1476
HindIII AAGCTT 5 cut(s) 381, 1297, 2216, 2452, 2785
HpaI GTTAAC 1 cut(s) 1476
HpaII CCGG 1 cut(s) 40
HphI GGTGA 2 cut(s) 29, 95
Hpy166II GTNNAC 6 cut(s) 45, 793, 925, 1476, 3150, 3319
Hpy188I TCNGA 9 cut(s) 452, 817, 1292, 2320, 2387, 2482, 2992, 3035, 3280
Hpy8I GTNNAC 6 cut(s) 45, 793, 925, 1476, 3150, 3319
Hpy99I CGWCG 2 cut(s) 1740, 1743
HpyCH4III ACNGT 5 cut(s) 293, 1166, 1387, 1724, 2778
HpyCH4IV ACGT 7 cut(s) 889, 1327, 1549, 1738, 2760, 2875, 3256
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 704, 967, 2626
HpySE526I ACGT 7 cut(s) 889, 1327, 1549, 1738, 2760, 2875, 3256
Hsp92I GRCGYC 1 cut(s) 1738
HspAI GCGC 1 cut(s) 403
Ksp22I TGATCA 3 cut(s) 100, 2802, 2859
KspAI GTTAAC 1 cut(s) 1476
LguI GCTCTTC 1 cut(s) 1191
LmnI GCTCC 4 cut(s) 170, 2583, 2634, 2943
Lsp1109I GCAGC 8 cut(s) 31, 753, 1207, 1743, 1836, 2004, 2226, 2274
LweI GCATC 5 cut(s) 43, 1561, 1818, 2723, 3129
MaeI CTAG 7 cut(s) 275, 1002, 1122, 1196, 1554, 2019, 2331
MaeII ACGT 7 cut(s) 889, 1327, 1549, 1738, 2760, 2875, 3256
MaeIII GTNAC 8 cut(s) 277, 1130, 1682, 2071, 2333, 2984, 3095, 3163
MflI RGATCY 8 cut(s) 25, 132, 340, 817, 1138, 1199, 1537, 3030
MhlI GDGCHC 5 cut(s) 47, 77, 312, 1629, 2631
MlyI GAGTC 7 cut(s) 218, 506, 579, 1664, 2088, 2315, 2593
MmeI TCCRAC 5 cut(s) 568, 628, 2023, 2440, 2574
Mph1103I ATGCAT 3 cut(s) 58, 1243, 3049
MroXI GAANNNNTTC 2 cut(s) 969, 1723
MslI CAYNNNNRTG 7 cut(s) 317, 1071, 1421, 1637, 2061, 2662, 2798
MspCI CTTAAG 1 cut(s) 2455
MspI CCGG 1 cut(s) 40
MspR9I CCNGG 6 cut(s) 266, 978, 1542, 1759, 2900, 2940
Mva1269I GAATGC 3 cut(s) 56, 963, 1428
MvaI CCWGG 6 cut(s) 266, 978, 1542, 1759, 2900, 2940
MvnI CGCG 1 cut(s) 1521
MwoI GCNNNNNNNGC 4 cut(s) 72, 704, 967, 2626
NmeAIII GCCGAG 1 cut(s) 2650
NmuCI GTSAC 4 cut(s) 277, 2071, 2333, 3163
NruI TCGCGA 1 cut(s) 1521
NsiI ATGCAT 3 cut(s) 58, 1243, 3049
NspI RCATGY 5 cut(s) 622, 1103, 2006, 3051, 3199
PaeI GCATGC 1 cut(s) 1103
PaeR7I CTCGAG 3 cut(s) 213, 253, 2105
PagI TCATGA 3 cut(s) 1027, 1318, 2211
PceI AGGCCT 2 cut(s) 612, 3039
PciI ACATGT 3 cut(s) 618, 2002, 3195
PciSI GCTCTTC 1 cut(s) 1191
PcsI WCGNNNNNNNCGW 1 cut(s) 886
PctI GAATGC 3 cut(s) 56, 963, 1428
PdmI GAANNNNTTC 2 cut(s) 969, 1723
PflMI CCANNNNNTGG 1 cut(s) 2905
PfoI TCCNGGA 2 cut(s) 976, 1540
PinAI ACCGGT 1 cut(s) 39
PkrI GCNGC 9 cut(s) 7, 21, 743, 1222, 1733, 1851, 1994, 2241, 2289
PleI GAGTC 7 cut(s) 217, 505, 578, 1663, 2088, 2315, 2592
PpsI GAGTC 7 cut(s) 217, 505, 578, 1663, 2088, 2315, 2592
Ppu21I YACGTR 2 cut(s) 2876, 3257
PpuMI RGGWCCY 1 cut(s) 2891
PscI ACATGT 3 cut(s) 618, 2002, 3195
PshAI GACNNNNGTC 1 cut(s) 1326
PshBI ATTAAT 1 cut(s) 2114
PsiI TTATAA 1 cut(s) 711
Psp124BI GAGCTC 1 cut(s) 2631
Psp5II RGGWCCY 1 cut(s) 2891
Psp6I CCWGG 6 cut(s) 264, 976, 1540, 1757, 2898, 2938
PspGI CCWGG 6 cut(s) 264, 976, 1540, 1757, 2898, 2938
PspPI GGNCC 4 cut(s) 289, 1618, 2891, 3150
PspPPI RGGWCCY 1 cut(s) 2891
PstI CTGCAG 2 cut(s) 1225, 2365
PstNI CAGNNNCTG 2 cut(s) 2324, 2905
PsuI RGATCY 8 cut(s) 25, 132, 340, 817, 1138, 1199, 1537, 3030
RruI TCGCGA 1 cut(s) 1521
RsaI GTAC 9 cut(s) 494, 792, 1552, 1603, 2328, 2668, 2685, 2878, 3320
RsaNI GTAC 9 cut(s) 493, 791, 1551, 1602, 2327, 2667, 2684, 2877, 3319
RseI CAYNNNNRTG 7 cut(s) 317, 1071, 1421, 1637, 2061, 2662, 2798
SacI GAGCTC 1 cut(s) 2631
SapI GCTCTTC 1 cut(s) 1191
SatI GCNGC 9 cut(s) 6, 20, 742, 1221, 1732, 1850, 1993, 2240, 2288
Sau96I GGNCC 4 cut(s) 289, 1618, 2891, 3150
SchI GAGTC 7 cut(s) 218, 506, 579, 1664, 2088, 2315, 2593
ScrFI CCNGG 6 cut(s) 266, 978, 1542, 1759, 2900, 2940
SduI GDGCHC 5 cut(s) 47, 77, 312, 1629, 2631
SfaNI GCATC 5 cut(s) 43, 1561, 1818, 2723, 3129
SfcI CTRYAG 5 cut(s) 657, 1152, 1221, 1383, 2361
Sfr274I CTCGAG 3 cut(s) 213, 253, 2105
SgrAI CRCCGGYG 1 cut(s) 39
SinI GGWCC 3 cut(s) 289, 2891, 3150
SlaI CTCGAG 3 cut(s) 213, 253, 2105
SmiMI CAYNNNNRTG 7 cut(s) 317, 1071, 1421, 1637, 2061, 2662, 2798
SmlI CTYRAG 6 cut(s) 61, 213, 253, 1082, 2105, 2455
SmoI CTYRAG 6 cut(s) 61, 213, 253, 1082, 2105, 2455
SpeI ACTAGT 1 cut(s) 274
SphI GCATGC 1 cut(s) 1103
SseBI AGGCCT 2 cut(s) 612, 3039
SsiI CCGC 1 cut(s) 1984
SspI AATATT 3 cut(s) 178, 1044, 1671
SspMI CTAG 7 cut(s) 275, 1002, 1122, 1196, 1554, 2019, 2331
SstI GAGCTC 1 cut(s) 2631
StuI AGGCCT 2 cut(s) 612, 3039
StyD4I CCNGG 6 cut(s) 264, 976, 1540, 1757, 2898, 2938
StyI CCWWGG 2 cut(s) 237, 3135
TaaI ACNGT 5 cut(s) 293, 1166, 1387, 1724, 2778
TaiI ACGT 7 cut(s) 892, 1330, 1552, 1741, 2763, 2878, 3259
TaqI TCGA 8 cut(s) 162, 214, 254, 573, 2106, 2613, 2790, 2975
TaqII GACCGA 2 cut(s) 2804, 3091
TatI WGTACW 3 cut(s) 790, 2666, 2683
TscAI CASTG 5 cut(s) 1169, 1681, 1876, 2431, 2851
TseFI GTSAC 4 cut(s) 277, 2071, 2333, 3163
TseI GCWGC 9 cut(s) 5, 19, 741, 1220, 1731, 1849, 1992, 2239, 2287
Tsp45I GTSAC 4 cut(s) 277, 2071, 2333, 3163
TspGWI ACGGA 1 cut(s) 18
TspRI CASTG 5 cut(s) 1169, 1681, 1876, 2431, 2851
Van91I CCANNNNNTGG 1 cut(s) 2905
Vha464I CTTAAG 1 cut(s) 2455
VneI GTGCAC 1 cut(s) 43
VpaK11BI GGWCC 3 cut(s) 289, 2891, 3150
VspI ATTAAT 1 cut(s) 2114
XagI CCTNNNNNAGG 1 cut(s) 1125
XapI RAATTY 9 cut(s) 369, 728, 1275, 1897, 2134, 2484, 2740, 2750, 3299
XceI RCATGY 5 cut(s) 622, 1103, 2006, 3051, 3199
XcmI CCANNNNNNNNNTGG 2 cut(s) 1954, 2164
XhoI CTCGAG 3 cut(s) 213, 253, 2105
XmnI GAANNNNTTC 2 cut(s) 969, 1723
XspI CTAG 7 cut(s) 275, 1002, 1122, 1196, 1554, 2019, 2331
ZraI GACGTC 1 cut(s) 1739
Zsp2I ATGCAT 3 cut(s) 58, 1243, 3049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.