FvH4_2g26661

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000673
Physical Location & Seq
Forward (+)
21373440 .. 21374849
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26661.t1

Sequence Viewer

Length: 1095 bp
ATGAGAAGAACAACAATTTCCGAATGGGTTCGACCTATTCTCAGTGGCCGCCGGCCGATGACGACTTCTGAAACAGAAGAAGAAAGACCTGGAGACGAAAAAAAGTCTTGTTGGTCCGACATACCTGTTGATATCTTATGTGAAGTGGCCGATCGTGTCCCATATTGGGATCTTTTTCGTTTTGCTACTGTATGCAAGACCTGGAGACTTGCCATATACAGGGTTCATCTTCCACAACGCTCCACATTGCTTGTGCCATACTTGTTGAGCTATACCTGTATACGTGAACGTGATGAATACATCGAGTTTTATGACCTGCATGGTGAAACTTACAAATACCCAATCGATAGCGAGAGACAACTCTTCGGCGGTACCAGCAGACTCTATGCTAGAAAATTTGGTTGGTTACTTTTGAAAAAGCTTGAGCTACCCTTTGAACAATTCTATCTGTTCAATCCTTTTTGGAATCTGGTTGTACTACTGCCCCCATTAGAGTTGGAGTTGGGTTTTCAAGTTGTATTAGCAACATTCACGAGCTCTCCAATACGCGGCTGTGTTATCTTGGTCATTGCCAGAAGGTATGGATCTAATGATCACATCATAAACACATGTAAGCTCCCAAAATTCGATTGGAGTGTTCACAAAACTAGATGTCAAGGTTCTGTTGAGGGAGTTGCTTATTTAGATGGGATTTTCTATTGTGTTGTTCCAAAGATAATTGATGATTTGAATTCTTTGTCGCTGTCGATCAGTACCTTCAATATAGCACTTCAAGCTTGGGTCATGGACTATGCCTCCACAACACTTCAACACCATACCCGAAGCTCAGCCTTTTGCGTGGTTGAGTCGGATGGGAAGCTATTATTAGCAGTTCGTGATGAACCAGTATTGCTTATATACCAGTTTGATTGGTTACGTAAAGATTGGATTAGGGTTCAAAATTTGGGGAACCATGTATTGCTTTTAGATCACTCATCATCAATTGCTTGTCCGACATTGGGGAGGGACAGTAAATTTTCAAACAAGATATTTCATCTTGACTGGGGCAAGGAGCCTTTTACTTTCAGCGGCAATGGTGTCTGGAGAAGGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

365

Amino Acids

42.38

Weight (kDa)

7.99

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 38 - 76 9.7e-09 F-box domain
F-box-like PF12937 38 - 75 4.2e-06 F-box-like
Beta-prop_KIB1-4 PF03478 124 - 347 2.3e-17 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 324
Acc65I GGTACC 1 cut(s) 371
AccB1I GGYRCC 1 cut(s) 371
AccI GTMKAC 1 cut(s) 280
AccII CGCG 1 cut(s) 549
AciI CCGC 4 cut(s) 49, 369, 549, 1068
AclWI GGATC 2 cut(s) 177, 592
AcoI YGGCCR 3 cut(s) 46, 53, 147
AcsI RAATTY 5 cut(s) 395, 623, 730, 940, 1013
AfaI GTAC 3 cut(s) 373, 477, 754
AfiI CCNNNNNNNGG 4 cut(s) 166, 219, 548, 998
AflIII ACRYGT 1 cut(s) 608
AjnI CCWGG 2 cut(s) 88, 200
AjuI GAANNNNNNNTTGG 2 cut(s) 385, 417
AluBI AGCT 8 cut(s) 270, 421, 427, 537, 616, 776, 825, 859
AluI AGCT 8 cut(s) 270, 421, 427, 537, 616, 776, 825, 859
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 3 cut(s) 87, 199, 349
AlwI GGATC 2 cut(s) 177, 592
AoxI GGCC 4 cut(s) 46, 53, 147, 1088
ApoI RAATTY 5 cut(s) 395, 623, 730, 940, 1013
Asp700I GAANNNNTTC 1 cut(s) 27
Asp718I GGTACC 1 cut(s) 371
AspS9I GGNCC 2 cut(s) 114, 1089
AsuHPI GGTGA 1 cut(s) 335
AvaII GGWCC 1 cut(s) 114
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 539
BauI CACGAG 1 cut(s) 532
Bbv12I GWGCWC 1 cut(s) 539
BccI CCATC 2 cut(s) 680, 845
BciT130I CCWGG 2 cut(s) 90, 202
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 3 cut(s) 87, 199, 349
BfaI CTAG 2 cut(s) 390, 648
BfuAI ACCTGC 1 cut(s) 324
BisI GCNGC 3 cut(s) 49, 550, 1069
BlpI GCTNAGC 1 cut(s) 826
BlsI GCNGC 3 cut(s) 50, 551, 1070
Bme1390I CCNGG 2 cut(s) 90, 202
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 2 cut(s) 114, 1089
BmiI GGNNCC 3 cut(s) 373, 950, 1053
BmrFI CCNGG 2 cut(s) 90, 202
BmrI ACTGGG 1 cut(s) 1051
BmuI ACTGGG 1 cut(s) 1051
BpmI CTGGAG 2 cut(s) 111, 223
Bpu1102I GCTNAGC 1 cut(s) 826
BpuEI CTTGAG 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 345
BsaAI YACGTR 2 cut(s) 284, 917
BsaXI ACNNNNNCTCC 6 cut(s) 523, 553, 779, 809, 994, 1024
Bsc4I CCNNNNNNNGG 4 cut(s) 166, 219, 548, 998
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 3 cut(s) 884, 901, 1046
Bse3DI GCAATG 3 cut(s) 245, 567, 1078
BseBI CCWGG 2 cut(s) 90, 202
BseCI ATCGAT 1 cut(s) 345
BseGI GGATG 1 cut(s) 856
BseLI CCNNNNNNNGG 4 cut(s) 166, 219, 548, 998
BseMI GCAATG 3 cut(s) 245, 567, 1078
BseMII CTCAG 2 cut(s) 55, 840
BseNI ACTGG 3 cut(s) 884, 901, 1046
BseX3I CGGCCG 1 cut(s) 53
Bsh1236I CGCG 1 cut(s) 549
Bsh1285I CGRYCG 2 cut(s) 56, 154
BshFI GGCC 4 cut(s) 48, 55, 149, 1090
BshNI GGYRCC 1 cut(s) 371
BshVI ATCGAT 1 cut(s) 345
BsiEI CGRYCG 2 cut(s) 56, 154
BsiHKAI GWGCWC 1 cut(s) 539
BsiSI CCGG 1 cut(s) 52
BslFI GGGAC 2 cut(s) 143, 1019
BslI CCNNNNNNNGG 4 cut(s) 166, 219, 548, 998
BsmAI GTCTC 3 cut(s) 87, 199, 349
BsmBI CGTCTC 1 cut(s) 87
BsmFI GGGAC 2 cut(s) 143, 1019
BsnI GGCC 4 cut(s) 48, 55, 149, 1090
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 6 cut(s) 151, 169, 584, 592, 747, 967
Bsp1720I GCTNAGC 1 cut(s) 826
BspACI CCGC 4 cut(s) 49, 369, 549, 1068
BspANI GGCC 4 cut(s) 48, 55, 149, 1090
BspCNI CTCAG 2 cut(s) 54, 839
BspDI ATCGAT 1 cut(s) 345
BspFNI CGCG 1 cut(s) 549
BspLI GGNNCC 3 cut(s) 373, 950, 1053
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 2 cut(s) 177, 592
BspT107I GGYRCC 1 cut(s) 371
BsrDI GCAATG 3 cut(s) 245, 567, 1078
BsrFI RCCGGY 1 cut(s) 51
BsrI ACTGG 3 cut(s) 884, 901, 1046
BssAI RCCGGY 1 cut(s) 51
BssMI GATC 6 cut(s) 151, 169, 584, 592, 747, 967
BssNAI GTATAC 1 cut(s) 281
BssSI CACGAG 1 cut(s) 532
Bst1107I GTATAC 1 cut(s) 281
Bst2BI CACGAG 1 cut(s) 532
Bst2UI CCWGG 2 cut(s) 90, 202
Bst4CI ACNGT 2 cut(s) 190, 1010
Bst6I CTCTTC 1 cut(s) 368
BstBAI YACGTR 2 cut(s) 284, 917
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 2 cut(s) 41, 826
BstF5I GGATG 1 cut(s) 856
BstFNI CGCG 1 cut(s) 549
BstKTI GATC 6 cut(s) 154, 172, 587, 595, 750, 970
BstMAI GTCTC 3 cut(s) 87, 199, 349
BstMBI GATC 6 cut(s) 151, 169, 584, 592, 747, 967
BstMCI CGRYCG 2 cut(s) 56, 154
BstMWI GCNNNNNNNGC 2 cut(s) 375, 773
BstNI CCWGG 2 cut(s) 90, 202
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 2 cut(s) 88, 200
BstSNI TACGTA 1 cut(s) 917
BstUI CGCG 1 cut(s) 549
BstX2I RGATCY 2 cut(s) 169, 584
BstYI RGATCY 2 cut(s) 169, 584
BstZ17I GTATAC 1 cut(s) 281
BstZI CGGCCG 1 cut(s) 53
Bsu15I ATCGAT 1 cut(s) 345
BsuRI GGCC 4 cut(s) 48, 55, 149, 1090
BsuTUI ATCGAT 1 cut(s) 345
BtsCI GGATG 1 cut(s) 856
BtsIMutI CAGTG 1 cut(s) 49
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 2 cut(s) 114, 1089
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 3 cut(s) 372, 476, 753
CspCI CAANNNNNGTGG 2 cut(s) 232, 267
CviAII CATG 4 cut(s) 320, 609, 784, 953
CviQI GTAC 3 cut(s) 372, 476, 753
DdeI CTNAG 2 cut(s) 41, 826
DpnI GATC 6 cut(s) 153, 171, 586, 594, 749, 969
DpnII GATC 6 cut(s) 151, 169, 584, 592, 747, 967
EaeI YGGCCR 3 cut(s) 46, 53, 147
EagI CGGCCG 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Ecl136II GAGCTC 1 cut(s) 537
EclXI CGGCCG 1 cut(s) 53
Eco105I TACGTA 1 cut(s) 917
Eco24I GRGCYC 1 cut(s) 539
Eco32I GATATC 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 114
Eco52I CGGCCG 1 cut(s) 53
Eco53kI GAGCTC 1 cut(s) 537
EcoICRI GAGCTC 1 cut(s) 537
EcoO109I RGGNCCY 1 cut(s) 1089
EcoRI GAATTC 1 cut(s) 730
EcoRII CCWGG 2 cut(s) 88, 200
EcoRV GATATC 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 539
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 4 cut(s) 323, 612, 787, 956
FaqI GGGAC 2 cut(s) 143, 1019
FatI CATG 4 cut(s) 319, 608, 783, 952
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 3 cut(s) 49, 550, 1069
FokI GGATG 1 cut(s) 863
FriOI GRGCYC 1 cut(s) 539
Fsp4HI GCNGC 3 cut(s) 49, 550, 1069
FspBI CTAG 2 cut(s) 390, 648
GluI GCNGC 3 cut(s) 49, 550, 1069
GsuI CTGGAG 2 cut(s) 111, 223
HaeIII GGCC 4 cut(s) 48, 55, 149, 1090
HapII CCGG 1 cut(s) 52
Hin1II CATG 4 cut(s) 323, 612, 787, 956
HindIII AAGCTT 2 cut(s) 419, 774
HinfI GANTC 3 cut(s) 381, 466, 845
HpaII CCGG 1 cut(s) 52
HphI GGTGA 1 cut(s) 335
Hpy166II GTNNAC 3 cut(s) 281, 287, 640
Hpy188I TCNGA 5 cut(s) 22, 70, 118, 850, 993
Hpy188III TCNNGA 4 cut(s) 532, 875, 1037, 1081
Hpy8I GTNNAC 3 cut(s) 281, 287, 640
HpyAV CCTTC 3 cut(s) 570, 766, 1080
HpyCH4III ACNGT 2 cut(s) 190, 1010
HpyCH4IV ACGT 3 cut(s) 283, 289, 916
HpyCH4V TGCA 2 cut(s) 195, 319
HpyF10VI GCNNNNNNNGC 2 cut(s) 375, 773
HpyF3I CTNAG 2 cut(s) 41, 826
HpySE526I ACGT 3 cut(s) 283, 289, 916
Hsp92II CATG 4 cut(s) 323, 612, 787, 956
KpnI GGTACC 1 cut(s) 375
KroI GCCGGC 1 cut(s) 51
KroNI GCCGGC 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 6 cut(s) 151, 169, 584, 592, 747, 967
LmnI GCTCC 3 cut(s) 245, 621, 1051
MaeI CTAG 2 cut(s) 390, 648
MaeII ACGT 3 cut(s) 283, 289, 916
MaeIII GTNAC 2 cut(s) 405, 912
MalI GATC 6 cut(s) 153, 171, 586, 594, 749, 969
MboI GATC 6 cut(s) 151, 169, 584, 592, 747, 967
MboII GAAGA 5 cut(s) 18, 89, 92, 221, 355
MfeI CAATTG 1 cut(s) 981
MflI RGATCY 2 cut(s) 169, 584
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 9 cut(s) 15, 395, 440, 623, 717, 730, 940, 981, 1013
MlyI GAGTC 2 cut(s) 375, 854
MmeI TCCRAC 4 cut(s) 141, 477, 828, 1016
MnlI CCTC 3 cut(s) 661, 805, 996
MroNI GCCGGC 1 cut(s) 51
MroXI GAANNNNTTC 1 cut(s) 27
MspA1I CMGCKG 1 cut(s) 1068
MspI CCGG 1 cut(s) 52
MspR9I CCNGG 2 cut(s) 90, 202
MunI CAATTG 1 cut(s) 981
MvaI CCWGG 2 cut(s) 90, 202
MvnI CGCG 1 cut(s) 549
MwoI GCNNNNNNNGC 2 cut(s) 375, 773
NaeI GCCGGC 1 cut(s) 53
NdeII GATC 6 cut(s) 151, 169, 584, 592, 747, 967
NgoMIV GCCGGC 1 cut(s) 51
NlaIII CATG 4 cut(s) 323, 612, 787, 956
NlaIV GGNNCC 3 cut(s) 373, 950, 1053
NspI RCATGY 1 cut(s) 612
PciI ACATGT 1 cut(s) 608
PdiI GCCGGC 1 cut(s) 53
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 1 cut(s) 466
PkrI GCNGC 3 cut(s) 50, 551, 1070
Ple19I CGATCG 1 cut(s) 154
PleI GAGTC 2 cut(s) 375, 853
PpsI GAGTC 2 cut(s) 375, 853
Ppu21I YACGTR 2 cut(s) 284, 917
PscI ACATGT 1 cut(s) 608
Psp124BI GAGCTC 1 cut(s) 539
Psp6I CCWGG 2 cut(s) 88, 200
PspGI CCWGG 2 cut(s) 88, 200
PspN4I GGNNCC 3 cut(s) 373, 950, 1053
PspPI GGNCC 2 cut(s) 114, 1089
PsuI RGATCY 2 cut(s) 169, 584
PvuI CGATCG 1 cut(s) 154
RsaI GTAC 3 cut(s) 373, 477, 754
RsaNI GTAC 3 cut(s) 372, 476, 753
SacI GAGCTC 1 cut(s) 539
SatI GCNGC 3 cut(s) 49, 550, 1069
Sau3AI GATC 6 cut(s) 151, 169, 584, 592, 747, 967
Sau96I GGNCC 2 cut(s) 114, 1089
SchI GAGTC 2 cut(s) 375, 854
ScrFI CCNGG 2 cut(s) 90, 202
SduI GDGCHC 1 cut(s) 539
SinI GGWCC 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SnaBI TACGTA 1 cut(s) 917
Sse9I AATT 9 cut(s) 15, 395, 440, 623, 717, 730, 940, 981, 1013
SsiI CCGC 4 cut(s) 49, 369, 549, 1068
SspMI CTAG 2 cut(s) 390, 648
SstI GAGCTC 1 cut(s) 539
StyD4I CCNGG 2 cut(s) 88, 200
TaaI ACNGT 2 cut(s) 190, 1010
TaiI ACGT 3 cut(s) 286, 292, 919
TaqI TCGA 5 cut(s) 31, 303, 345, 627, 746
TasI AATT 9 cut(s) 15, 395, 440, 623, 717, 730, 940, 981, 1013
TatI WGTACW 1 cut(s) 475
TauI GCSGC 3 cut(s) 51, 552, 1071
TfiI GAWTC 1 cut(s) 466
TscAI CASTG 1 cut(s) 49
TspDTI ATGAA 4 cut(s) 215, 309, 894, 1022
TspRI CASTG 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 114
XapI RAATTY 5 cut(s) 395, 623, 730, 940, 1013
XceI RCATGY 1 cut(s) 612
XcmI CCANNNNNNNNNTGG 1 cut(s) 627
XmiI GTMKAC 1 cut(s) 280
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 2 cut(s) 390, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.