Rw6G031400

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000673
Physical Location & Seq
Reverse (-)
55379935 .. 55381128
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G031400.1

Sequence Viewer

Length: 1194 bp
ATGGTAGACAATAATCTTTCTTCATGGTCAGATCCTCCAATCGATATCTTATGCGCGGTAGTTATTCGTCTCCTCTCCGTATGCGAGTATAAAGATCTGGTTCGTCTTTCTGCTGTATGCAAGAGCTGGAGATTTATTATATATAGGGTCTATCTTCAACCAATCGATATTTTATGCGAGGTCGCTAATCGTCTCCAATATGAAGAACTCGTTCGTCTTTCTGCTGTTTGCAAGATCTGGAGATTTATTATATATAGGGTCTATCTTCAACTACGCTCCCCTCTCCGTATGCCAGGCCTGACGCTATTCAGTCCATATCATCAAGACGAACACGGCCGGATAATAAGCTTCTTTGATATATCAATCCAGAAAATCCGCCGTATAAAGCTTAAGATTCGAGAAGAATTAAAAACATTCTGGCAACTTGATTCAAAATATGGTTGGATCATTTTGCAAAACGGGTGCGAATCCAAACCTGCACTCGTCTTGTATAATCCTTTAACCAAAGAGATTATACAACTGCCACAGTTGAACCTAAGCACCAATAGTGAATGTATAGCAACATTCACAAGCCCTCCGACTTCTCCTGACTGTGTCATCTTTGCCTTCTTCCGAGTAGAAAGAGGAGAAGCTTGCATTGTAGGCACATGTAAGCTGGGAGATAACATATGGAGATTTCGAAAAATAACTGACTTTAAGGGTTGGAAGGGCAGGAACTACGTTGCGAATGTGACCTATTTCAATGGAATTTTCTGTTTTGTTATTCAAACGTGTGAACCTGATTGGTTGGTAGAGTTGGGTACCTTGGATGTATCGGTTCAAGACTGGGTCATGGAATATGCTTCCATAACAGTAGCACAAGAACAGAATCAGTATTGGGAGTGGTATTGGCTGGTTGAGTCTGATGGGAAGCTGTTATTGGCAGTTTATCATAGCCATGGGGATGTTGCAATGACCTATTGGCATGTTTACCAATTCGATTGGTCACGAAAGGATTGGACTAGGGTTCGAGGTTTAGGAAACCAGGTATTGATTTTACGTCGACATGATTGGTCCAGGTTGACATCATTTGCTTGTCCCACATCCGGAGACGATAGTAAACTTTCAAACATGATATTTTGTGACGCCACCAGACGCGGTGCCCATTTTTATACTTGCAGCAGTGATGGCGTTTGGAGAATCGCTAGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

397

Amino Acids

46.5

Weight (kDa)

8.28

Isoelectric Point (pI)

35.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 54 - 87 3.5e-06 F-box domain
Beta-prop_KIB1-4 PF03478 117 - 351 1.2e-21 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 484
Acc65I GGTACC 1 cut(s) 800
AccB1I GGYRCC 2 cut(s) 800, 1139
AccI GTMKAC 2 cut(s) 6, 1042
AccII CGCG 2 cut(s) 56, 1137
AccIII TCCGGA 1 cut(s) 1085
AciI CCGC 3 cut(s) 56, 376, 1137
AclWI GGATC 2 cut(s) 26, 452
AcoI YGGCCR 1 cut(s) 334
AcsI RAATTY 1 cut(s) 747
AcyI GRCGYC 1 cut(s) 1125
AfaI GTAC 1 cut(s) 802
AfiI CCNNNNNNNGG 1 cut(s) 1085
AflII CTTAAG 1 cut(s) 389
AflIII ACRYGT 2 cut(s) 647, 770
AgsI TTSAA 8 cut(s) 158, 269, 432, 532, 742, 767, 821, 1107
AjnI CCWGG 3 cut(s) 292, 1023, 1055
AjuI GAANNNNNNNTTGG 2 cut(s) 902, 934
AluBI AGCT 6 cut(s) 126, 348, 388, 632, 655, 913
AluI AGCT 6 cut(s) 126, 348, 388, 632, 655, 913
Alw26I GTCTC 3 cut(s) 74, 197, 1083
AlwI GGATC 2 cut(s) 26, 452
Aor13HI TCCGGA 1 cut(s) 1085
AoxI GGCC 2 cut(s) 295, 334
ApeKI GCWGC 1 cut(s) 1158
ApoI RAATTY 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 210
Asp718I GGTACC 1 cut(s) 800
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 1053
AsuII TTCGAA 1 cut(s) 679
AsuNHI GCTAGC 1 cut(s) 1184
AvaII GGWCC 1 cut(s) 1053
BaeGI GKGCMC 1 cut(s) 1144
BanI GGYRCC 2 cut(s) 800, 1139
BbvI GCAGC 1 cut(s) 1170
BccI CCATC 2 cut(s) 899, 1160
BceAI ACGGC 2 cut(s) 349, 363
BciT130I CCWGG 3 cut(s) 294, 1025, 1057
BcoDI GTCTC 3 cut(s) 74, 197, 1083
BfaI CTAG 2 cut(s) 1002, 1185
BfrI CTTAAG 1 cut(s) 389
BfuAI ACCTGC 1 cut(s) 484
BglII AGATCT 2 cut(s) 94, 234
BisI GCNGC 1 cut(s) 1159
BlsI GCNGC 1 cut(s) 1160
Bme1390I CCNGG 3 cut(s) 294, 1025, 1057
Bme18I GGWCC 1 cut(s) 1053
BmgT120I GGNCC 1 cut(s) 1053
BmiI GGNNCC 2 cut(s) 802, 1141
BmrFI CCNGG 3 cut(s) 294, 1025, 1057
BmrI ACTGGG 1 cut(s) 835
BmtI GCTAGC 1 cut(s) 1188
BmuI ACTGGG 1 cut(s) 835
BpmI CTGGAG 2 cut(s) 148, 259
Bpu10I CCTNAGC 1 cut(s) 536
Bpu14I TTCGAA 1 cut(s) 679
Bsa29I ATCGAT 2 cut(s) 42, 165
BsaHI GRCGYC 1 cut(s) 1125
BsaJI CCNNGG 2 cut(s) 804, 937
BsaWI WCCGGW 1 cut(s) 1085
BsaXI ACNNNNNCTCC 2 cut(s) 559, 589
Bsc4I CCNNNNNNNGG 1 cut(s) 1085
Bse1I ACTGG 1 cut(s) 830
Bse3DI GCAATG 1 cut(s) 957
BseAI TCCGGA 1 cut(s) 1085
BseBI CCWGG 3 cut(s) 294, 1025, 1057
BseCI ATCGAT 2 cut(s) 42, 165
BseDI CCNNGG 2 cut(s) 804, 937
BseGI GGATG 3 cut(s) 814, 949, 1082
BseLI CCNNNNNNNGG 1 cut(s) 1085
BseMI GCAATG 1 cut(s) 957
BseNI ACTGG 1 cut(s) 830
BseRI GAGGAG 2 cut(s) 62, 639
BseSI GKGCMC 1 cut(s) 1144
BseX3I CGGCCG 1 cut(s) 334
BseXI GCAGC 1 cut(s) 1170
BseYI CCCAGC 1 cut(s) 655
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 2 cut(s) 56, 1137
Bsh1285I CGRYCG 1 cut(s) 337
BshFI GGCC 2 cut(s) 297, 336
BshNI GGYRCC 2 cut(s) 800, 1139
BshVI ATCGAT 2 cut(s) 42, 165
BsiEI CGRYCG 1 cut(s) 337
BsiSI CCGG 2 cut(s) 337, 1086
BslFI GGGAC 1 cut(s) 1062
BslI CCNNNNNNNGG 1 cut(s) 1085
BsmAI GTCTC 3 cut(s) 74, 197, 1083
BsmBI CGTCTC 3 cut(s) 74, 197, 1083
BsmFI GGGAC 1 cut(s) 1062
BsnI GGCC 2 cut(s) 297, 336
Bsp119I TTCGAA 1 cut(s) 679
Bsp1286I GDGCHC 1 cut(s) 1144
Bsp13I TCCGGA 1 cut(s) 1085
Bsp143I GATC 4 cut(s) 31, 94, 234, 444
Bsp19I CCATGG 1 cut(s) 937
BspACI CCGC 3 cut(s) 56, 376, 1137
BspANI GGCC 2 cut(s) 297, 336
BspDI ATCGAT 2 cut(s) 42, 165
BspEI TCCGGA 1 cut(s) 1085
BspFNI CGCG 2 cut(s) 56, 1137
BspLI GGNNCC 2 cut(s) 802, 1141
BspMI ACCTGC 1 cut(s) 484
BspOI GCTAGC 1 cut(s) 1188
BspPI GGATC 2 cut(s) 26, 452
BspT104I TTCGAA 1 cut(s) 679
BspT107I GGYRCC 2 cut(s) 800, 1139
BspTI CTTAAG 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 957
BsrI ACTGG 1 cut(s) 830
BssECI CCNNGG 2 cut(s) 804, 937
BssMI GATC 4 cut(s) 31, 94, 234, 444
BssNI GRCGYC 1 cut(s) 1125
BssT1I CCWWGG 2 cut(s) 804, 937
Bst2UI CCWGG 3 cut(s) 294, 1025, 1057
Bst4CI ACNGT 3 cut(s) 528, 593, 853
BstACI GRCGYC 1 cut(s) 1125
BstAFI CTTAAG 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 679
BstC8I GCNNGC 2 cut(s) 634, 1186
BstDEI CTNAG 1 cut(s) 536
BstDSI CCRYGG 1 cut(s) 937
BstF5I GGATG 3 cut(s) 814, 949, 1082
BstFNI CGCG 2 cut(s) 56, 1137
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 4 cut(s) 34, 97, 237, 447
BstMAI GTCTC 3 cut(s) 74, 197, 1083
BstMBI GATC 4 cut(s) 31, 94, 234, 444
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 2 cut(s) 642, 1167
BstNI CCWGG 3 cut(s) 294, 1025, 1057
BstNSI RCATGY 2 cut(s) 651, 968
BstSCI CCNGG 3 cut(s) 292, 1023, 1055
BstSLI GKGCMC 1 cut(s) 1144
BstUI CGCG 2 cut(s) 56, 1137
BstV1I GCAGC 1 cut(s) 1170
BstX2I RGATCY 3 cut(s) 31, 94, 234
BstYI RGATCY 3 cut(s) 31, 94, 234
BstZI CGGCCG 1 cut(s) 334
Bsu15I ATCGAT 2 cut(s) 42, 165
BsuRI GGCC 2 cut(s) 297, 336
BsuTUI ATCGAT 2 cut(s) 42, 165
BtgI CCRYGG 1 cut(s) 937
BtsCI GGATG 3 cut(s) 814, 949, 1082
BtsI GCAGTG 1 cut(s) 1168
BtsIMutI CAGTG 1 cut(s) 1168
BveI ACCTGC 1 cut(s) 484
Cac8I GCNNGC 2 cut(s) 634, 1186
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 1053
ClaI ATCGAT 2 cut(s) 42, 165
CseI GACGC 3 cut(s) 310, 1133, 1143
CsiI ACCWGGT 1 cut(s) 1023
Csp6I GTAC 1 cut(s) 801
CviAII CATG 7 cut(s) 24, 648, 832, 938, 965, 1046, 1111
CviQI GTAC 1 cut(s) 801
DdeI CTNAG 1 cut(s) 536
DpnI GATC 4 cut(s) 33, 96, 236, 446
DpnII GATC 4 cut(s) 31, 94, 234, 444
EaeI YGGCCR 1 cut(s) 334
EagI CGGCCG 1 cut(s) 334
EciI GGCGGA 1 cut(s) 365
EclXI CGGCCG 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 804, 937
Eco147I AGGCCT 1 cut(s) 297
Eco32I GATATC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 1053
Eco52I CGGCCG 1 cut(s) 334
EcoRII CCWGG 3 cut(s) 292, 1023, 1055
EcoRV GATATC 1 cut(s) 46
EcoT14I CCWWGG 2 cut(s) 804, 937
ErhI CCWWGG 2 cut(s) 804, 937
Esp3I CGTCTC 3 cut(s) 74, 197, 1083
FaeI CATG 7 cut(s) 27, 651, 835, 941, 968, 1049, 1114
FaqI GGGAC 1 cut(s) 1062
FatI CATG 7 cut(s) 23, 647, 831, 937, 964, 1045, 1110
FauNDI CATATG 1 cut(s) 668
FblI GTMKAC 2 cut(s) 6, 1042
Fnu4HI GCNGC 1 cut(s) 1159
FokI GGATG 3 cut(s) 821, 956, 1069
Fsp4HI GCNGC 1 cut(s) 1159
FspBI CTAG 2 cut(s) 1002, 1185
GlaI GCGC 1 cut(s) 55
GluI GCNGC 1 cut(s) 1159
GsaI CCCAGC 1 cut(s) 659
GsuI CTGGAG 2 cut(s) 148, 259
HaeIII GGCC 2 cut(s) 297, 336
HapII CCGG 2 cut(s) 337, 1086
HgaI GACGC 3 cut(s) 310, 1133, 1143
HhaI GCGC 1 cut(s) 56
Hin1I GRCGYC 1 cut(s) 1125
Hin1II CATG 7 cut(s) 27, 651, 835, 941, 968, 1049, 1114
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 2 cut(s) 1043, 1062
HindII GTYRAC 2 cut(s) 1043, 1062
HindIII AAGCTT 3 cut(s) 346, 386, 630
HinfI GANTC 6 cut(s) 394, 428, 467, 868, 899, 1179
HpaII CCGG 2 cut(s) 337, 1086
Hpy166II GTNNAC 6 cut(s) 7, 776, 970, 1043, 1062, 1100
Hpy188I TCNGA 4 cut(s) 31, 579, 614, 904
Hpy188III TCNNGA 8 cut(s) 238, 323, 367, 398, 587, 821, 987, 1086
Hpy8I GTNNAC 6 cut(s) 7, 776, 970, 1043, 1062, 1100
Hpy99I CGWCG 1 cut(s) 1044
HpyAV CCTTC 2 cut(s) 616, 700
HpyCH4III ACNGT 3 cut(s) 528, 593, 853
HpyCH4IV ACGT 3 cut(s) 720, 770, 1039
HpyCH4V TGCA 7 cut(s) 120, 231, 454, 479, 636, 950, 1158
HpyF10VI GCNNNNNNNGC 2 cut(s) 642, 1167
HpyF3I CTNAG 1 cut(s) 536
HpySE526I ACGT 3 cut(s) 720, 770, 1039
Hsp92I GRCGYC 1 cut(s) 1125
Hsp92II CATG 7 cut(s) 27, 651, 835, 941, 968, 1049, 1114
HspAI GCGC 1 cut(s) 54
Kpn2I TCCGGA 1 cut(s) 1085
KpnI GGTACC 1 cut(s) 804
Kzo9I GATC 4 cut(s) 31, 94, 234, 444
LmnI GCTCC 1 cut(s) 281
Lsp1109I GCAGC 1 cut(s) 1170
MabI ACCWGGT 1 cut(s) 1023
MaeI CTAG 2 cut(s) 1002, 1185
MaeII ACGT 3 cut(s) 720, 770, 1039
MaeIII GTNAC 3 cut(s) 730, 984, 1121
MalI GATC 4 cut(s) 33, 96, 236, 446
MboI GATC 4 cut(s) 31, 94, 234, 444
MboII GAAGA 6 cut(s) 12, 146, 215, 257, 413, 601
MflI RGATCY 3 cut(s) 31, 94, 234
MhlI GDGCHC 1 cut(s) 1144
MluCI AATT 3 cut(s) 404, 747, 974
MlyI GAGTC 1 cut(s) 908
MmeI TCCRAC 3 cut(s) 422, 602, 683
MnlI CCTC 7 cut(s) 45, 83, 172, 291, 585, 617, 1004
MroI TCCGGA 1 cut(s) 1085
MroXI GAANNNNTTC 1 cut(s) 210
MseI TTAA 4 cut(s) 390, 407, 500, 696
MslI CAYNNNNRTG 2 cut(s) 936, 942
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 2 cut(s) 337, 1086
MspR9I CCNGG 3 cut(s) 294, 1025, 1057
MvaI CCWGG 3 cut(s) 294, 1025, 1057
MvnI CGCG 2 cut(s) 56, 1137
MwoI GCNNNNNNNGC 2 cut(s) 642, 1167
NcoI CCATGG 1 cut(s) 937
NdeI CATATG 1 cut(s) 668
NdeII GATC 4 cut(s) 31, 94, 234, 444
NheI GCTAGC 1 cut(s) 1184
NlaIII CATG 7 cut(s) 27, 651, 835, 941, 968, 1049, 1114
NlaIV GGNNCC 2 cut(s) 802, 1141
NmuCI GTSAC 3 cut(s) 730, 984, 1121
NspI RCATGY 2 cut(s) 651, 968
NspV TTCGAA 1 cut(s) 679
PceI AGGCCT 1 cut(s) 297
PciI ACATGT 1 cut(s) 647
PdmI GAANNNNTTC 1 cut(s) 210
PfeI GAWTC 5 cut(s) 394, 428, 467, 868, 1179
PflFI GACNNNGTC 2 cut(s) 593, 827
PkrI GCNGC 1 cut(s) 1160
PleI GAGTC 1 cut(s) 907
PpsI GAGTC 1 cut(s) 907
PscI ACATGT 1 cut(s) 647
Psp6I CCWGG 3 cut(s) 292, 1023, 1055
PspFI CCCAGC 1 cut(s) 655
PspGI CCWGG 3 cut(s) 292, 1023, 1055
PspN4I GGNNCC 2 cut(s) 802, 1141
PspPI GGNCC 1 cut(s) 1053
PsuI RGATCY 3 cut(s) 31, 94, 234
PsyI GACNNNGTC 2 cut(s) 593, 827
RsaI GTAC 1 cut(s) 802
RsaNI GTAC 1 cut(s) 801
RseI CAYNNNNRTG 2 cut(s) 936, 942
SalI GTCGAC 1 cut(s) 1041
SaqAI TTAA 4 cut(s) 390, 407, 500, 696
SatI GCNGC 1 cut(s) 1159
Sau3AI GATC 4 cut(s) 31, 94, 234, 444
Sau96I GGNCC 1 cut(s) 1053
SchI GAGTC 1 cut(s) 908
ScrFI CCNGG 3 cut(s) 294, 1025, 1057
SduI GDGCHC 1 cut(s) 1144
SexAI ACCWGGT 1 cut(s) 1023
SfuI TTCGAA 1 cut(s) 679
SinI GGWCC 1 cut(s) 1053
SmiMI CAYNNNNRTG 2 cut(s) 936, 942
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 3 cut(s) 404, 747, 974
SseBI AGGCCT 1 cut(s) 297
SsiI CCGC 3 cut(s) 56, 376, 1137
SspMI CTAG 2 cut(s) 1002, 1185
StuI AGGCCT 1 cut(s) 297
StyD4I CCNGG 3 cut(s) 292, 1023, 1055
StyI CCWWGG 2 cut(s) 804, 937
TaaI ACNGT 3 cut(s) 528, 593, 853
TaiI ACGT 3 cut(s) 723, 773, 1042
TaqI TCGA 7 cut(s) 42, 165, 397, 679, 978, 1009, 1042
TasI AATT 3 cut(s) 404, 747, 974
TfiI GAWTC 5 cut(s) 394, 428, 467, 868, 1179
Tru1I TTAA 4 cut(s) 390, 407, 500, 696
Tru9I TTAA 4 cut(s) 390, 407, 500, 696
TscAI CASTG 1 cut(s) 1168
TseFI GTSAC 3 cut(s) 730, 984, 1121
TseI GCWGC 1 cut(s) 1158
Tsp45I GTSAC 3 cut(s) 730, 984, 1121
TspDTI ATGAA 2 cut(s) 12, 216
TspGWI ACGGA 2 cut(s) 67, 275
TspRI CASTG 1 cut(s) 1168
Tth111I GACNNNGTC 2 cut(s) 593, 827
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 1053
XapI RAATTY 1 cut(s) 747
XceI RCATGY 2 cut(s) 651, 968
XmiI GTMKAC 2 cut(s) 6, 1042
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 2 cut(s) 1002, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.