Rw3G004540

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000673
Physical Location & Seq
Reverse (-)
3917706 .. 3918875
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004540.1

Sequence Viewer

Length: 1170 bp
ATGCCAATTGAAATCTTAACCTCAGTTGCTGAACGTCTCTCACCTACACCAATCACTGCTGATATCAATCTTTTTCACTTTGGTTCCGTCTGCAAAACTTGGCGACAAGCTAGCTTTCGTGCAGGAGTTCGACGTCGTTCAAGTTTGCCATGGCTAATTAACGTCGAATACGAGTTGATTTGCAGAAACTTACCAGCATTGAAGTGTCAGTTCTCCGATTTCTGGTCCAAACAAATTTTATACTCCTATGCAGAAGTCTTACCGAAAAAATTGTGGAGAAACCCTACTGCACATGCTTCAAAAAATGGCTGGTTGCTTTTGTCGACGGATGAGTGTATTAATCCTATTTTGGCAAGTACCTCATTCTTTTTTTTTAATCCTTTTCGTGAGAAGATGGCGATCATCGAGTTACCTGAAGATTTGTATTTTTCACTCATTTACGAAATAGTAGAATCAACGTTTACAGCAAATCCAACTTGTCCCAATGATTGTCATATCTTTGTAGCGTGTGGTAGATCATCTAACCCTCGAAAAATTTGCAGCATATATACTTGTAATCTGCAGGAAGGTGAATGGAAAGAACAGTATATAGACTGTGACATGGAACTATTTACTGTGGCATATATGGATGGAGCTTTCTATGGCGTTTTTTCAGACGGGATTTCGATAGTACTGCATGTGTTCACATTTAGAAGACGGAGCTTTCTATCGCGTCTTAAGAAAGCCATTGGACAAGCTGATGATGGTGAACAATGTGATCCATTCGTTATCTCCTCCTCCTCCTCTTCCTTAAGAAAGAAGGAGAATGATGATCTATACTTAGTTGAGGCTGATGGGGATCTCTTATTAGCAATTTGTTCTATTGATGACACTCCCAGTTATTGTCACATATACCGGTTGGATCCGTCACGTAAAAAATGGAATCGAATTAGGAACTTGGGAAATCGAGTAATGATTTTGTCTTGCGATGCTGTTGGATCGTTTGTAGTTCCTGCAGTGCGAGATGCTAAAGAATTTGCAAATACGGTCTTTATTTATCGAGACTCAAGTAATGCTCATGCTATCTGCGAAAGTACTGAAGCCACTTGGATATTTGACAAAAAAATCCATCATGTAAATCCTTCTGAAGCAGGCACCACTCTCATTGAACCCCCTGACTTATGGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

389

Amino Acids

44.44

Weight (kDa)

5.89

Isoelectric Point (pI)

54.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 87 - 334 9.2e-20 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 136
AccB1I GGYRCC 1 cut(s) 1133
AccI GTMKAC 1 cut(s) 323
AccII CGCG 1 cut(s) 712
AclI AACGTT 1 cut(s) 458
AclWI GGATC 5 cut(s) 752, 846, 896, 909, 985
AcsI RAATTY 3 cut(s) 234, 534, 1013
AcuI CTGAAG 3 cut(s) 435, 1098, 1146
AcyI GRCGYC 1 cut(s) 133
AfaI GTAC 3 cut(s) 358, 672, 1075
AfiI CCNNNNNNNGG 1 cut(s) 222
AflII CTTAAG 2 cut(s) 716, 790
AgeI ACCGGT 1 cut(s) 894
AgsI TTSAA 5 cut(s) 11, 141, 202, 300, 1148
AluBI AGCT 5 cut(s) 110, 114, 635, 702, 737
AluI AGCT 5 cut(s) 110, 114, 635, 702, 737
Alw26I GTCTC 2 cut(s) 41, 1035
AlwI GGATC 5 cut(s) 752, 846, 896, 909, 985
AlwNI CAGNNNCTG 1 cut(s) 29
ApeKI GCWGC 1 cut(s) 540
ApoI RAATTY 3 cut(s) 234, 534, 1013
AseI ATTAAT 1 cut(s) 339
AsiGI ACCGGT 1 cut(s) 894
AspS9I GGNCC 1 cut(s) 225
AsuHPI GGTGA 3 cut(s) 33, 581, 758
AsuNHI GCTAGC 1 cut(s) 110
AvaII GGWCC 1 cut(s) 225
BamHI GGATCC 1 cut(s) 901
BanI GGYRCC 1 cut(s) 1133
BbsI GAAGAC 1 cut(s) 700
BbvI GCAGC 1 cut(s) 552
BccI CCATC 5 cut(s) 388, 623, 737, 827, 1116
BcgI CGANNNNNNTGC 4 cut(s) 519, 553, 655, 689
BcoDI GTCTC 2 cut(s) 41, 1035
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 2 cut(s) 560, 993
BfrI CTTAAG 2 cut(s) 716, 790
BisI GCNGC 1 cut(s) 541
BlsI GCNGC 1 cut(s) 542
BmcAI AGTACT 2 cut(s) 672, 1075
Bme18I GGWCC 1 cut(s) 225
BmgT120I GGNCC 1 cut(s) 225
BmiI GGNNCC 3 cut(s) 85, 903, 1135
BmrI ACTGGG 1 cut(s) 870
BmsI GCATC 2 cut(s) 958, 994
BmtI GCTAGC 1 cut(s) 114
BmuI ACTGGG 1 cut(s) 870
BpiI GAAGAC 1 cut(s) 700
BpuEI CTTGAG 1 cut(s) 1030
BsaAI YACGTR 1 cut(s) 911
BsaBI GATNNNNATC 3 cut(s) 66, 398, 837
BsaHI GRCGYC 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 149
BsaWI WCCGGW 1 cut(s) 894
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse118I RCCGGY 1 cut(s) 894
Bse1I ACTGG 1 cut(s) 876
Bse8I GATNNNNATC 3 cut(s) 66, 398, 837
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 2 cut(s) 334, 634
BseJI GATNNNNATC 3 cut(s) 66, 398, 837
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMII CTCAG 1 cut(s) 36
BseNI ACTGG 1 cut(s) 876
BseRI GAGGAG 4 cut(s) 763, 766, 769, 772
BseXI GCAGC 1 cut(s) 552
BsgI GTGCAG 2 cut(s) 141, 273
Bsh1236I CGCG 1 cut(s) 712
BshNI GGYRCC 1 cut(s) 1133
BshTI ACCGGT 1 cut(s) 894
BsiSI CCGG 1 cut(s) 895
BslFI GGGAC 1 cut(s) 465
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 2 cut(s) 41, 1035
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 465
Bsp143I GATC 7 cut(s) 399, 515, 757, 811, 838, 901, 977
Bsp19I CCATGG 1 cut(s) 149
BspCNI CTCAG 1 cut(s) 35
BspFNI CGCG 1 cut(s) 712
BspLI GGNNCC 3 cut(s) 85, 903, 1135
BspMAI CTGCAG 2 cut(s) 564, 997
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 5 cut(s) 752, 846, 896, 909, 985
BspT107I GGYRCC 1 cut(s) 1133
BspTI CTTAAG 2 cut(s) 716, 790
BsrFI RCCGGY 1 cut(s) 894
BsrI ACTGG 1 cut(s) 876
BssAI RCCGGY 1 cut(s) 894
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 7 cut(s) 399, 515, 757, 811, 838, 901, 977
BssNI GRCGYC 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 149
Bst4CI ACNGT 4 cut(s) 585, 596, 616, 1027
Bst6I CTCTTC 1 cut(s) 790
BstACI GRCGYC 1 cut(s) 133
BstAFI CTTAAG 2 cut(s) 716, 790
BstBAI YACGTR 1 cut(s) 911
BstC8I GCNNGC 2 cut(s) 112, 1132
BstDEI CTNAG 2 cut(s) 22, 820
BstDSI CCRYGG 1 cut(s) 149
BstF5I GGATG 2 cut(s) 334, 634
BstFNI CGCG 1 cut(s) 712
BstKTI GATC 7 cut(s) 402, 518, 760, 814, 841, 904, 980
BstMAI GTCTC 2 cut(s) 41, 1035
BstMBI GATC 7 cut(s) 399, 515, 757, 811, 838, 901, 977
BstNSI RCATGY 2 cut(s) 296, 680
BstSFI CTRYAG 2 cut(s) 560, 993
BstUI CGCG 1 cut(s) 712
BstV1I GCAGC 1 cut(s) 552
BstV2I GAAGAC 1 cut(s) 700
BstX2I RGATCY 2 cut(s) 838, 901
BstYI RGATCY 2 cut(s) 838, 901
BtgI CCRYGG 1 cut(s) 149
BtgZI GCGATG 1 cut(s) 981
BtsCI GGATG 2 cut(s) 334, 634
BtsI GCAGTG 2 cut(s) 54, 1002
BtsIMutI CAGTG 2 cut(s) 54, 1002
Cac8I GCNNGC 2 cut(s) 112, 1132
CaiI CAGNNNCTG 1 cut(s) 29
Cfr10I RCCGGY 1 cut(s) 894
Cfr13I GGNCC 1 cut(s) 225
CseI GACGC 1 cut(s) 701
Csp6I GTAC 3 cut(s) 357, 671, 1074
CspAI ACCGGT 1 cut(s) 894
CspCI CAANNNNNGTGG 2 cut(s) 1126, 1161
CviAII CATG 6 cut(s) 150, 293, 601, 677, 1058, 1112
CviQI GTAC 3 cut(s) 357, 671, 1074
DdeI CTNAG 2 cut(s) 22, 820
DpnI GATC 7 cut(s) 401, 517, 759, 813, 840, 903, 979
DpnII GATC 7 cut(s) 399, 515, 757, 811, 838, 901, 977
Eam1104I CTCTTC 1 cut(s) 790
EarI CTCTTC 1 cut(s) 790
Eco130I CCWWGG 1 cut(s) 149
Eco32I GATATC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 225
Eco57I CTGAAG 3 cut(s) 435, 1098, 1146
EcoRV GATATC 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 41
FaeI CATG 6 cut(s) 153, 296, 604, 680, 1061, 1115
FaqI GGGAC 1 cut(s) 465
FatI CATG 6 cut(s) 149, 292, 600, 676, 1057, 1111
FblI GTMKAC 1 cut(s) 323
Fnu4HI GCNGC 1 cut(s) 541
FokI GGATG 2 cut(s) 341, 641
Fsp4HI GCNGC 1 cut(s) 541
FspBI CTAG 1 cut(s) 111
GluI GCNGC 1 cut(s) 541
HapII CCGG 1 cut(s) 895
HgaI GACGC 1 cut(s) 701
Hin1I GRCGYC 1 cut(s) 133
Hin1II CATG 6 cut(s) 153, 296, 604, 680, 1061, 1115
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HinfI GANTC 3 cut(s) 452, 922, 1043
HpaII CCGG 1 cut(s) 895
HphI GGTGA 3 cut(s) 33, 581, 758
Hpy166II GTNNAC 4 cut(s) 324, 462, 684, 749
Hpy188I TCNGA 3 cut(s) 217, 655, 1126
Hpy188III TCNNGA 2 cut(s) 386, 1040
Hpy8I GTNNAC 4 cut(s) 324, 462, 684, 749
Hpy99I CGWCG 4 cut(s) 135, 138, 167, 328
HpyAV CCTTC 3 cut(s) 560, 793, 1131
HpyCH4III ACNGT 4 cut(s) 585, 596, 616, 1027
HpyCH4IV ACGT 5 cut(s) 34, 133, 162, 458, 910
HpyF3I CTNAG 2 cut(s) 22, 820
HpySE526I ACGT 5 cut(s) 34, 133, 162, 458, 910
Hsp92I GRCGYC 1 cut(s) 133
Hsp92II CATG 6 cut(s) 153, 296, 604, 680, 1061, 1115
Kzo9I GATC 7 cut(s) 399, 515, 757, 811, 838, 901, 977
LmnI GCTCC 2 cut(s) 632, 699
Lsp1109I GCAGC 1 cut(s) 552
LweI GCATC 2 cut(s) 958, 994
MaeI CTAG 1 cut(s) 111
MaeII ACGT 5 cut(s) 34, 133, 162, 458, 910
MaeIII GTNAC 4 cut(s) 408, 596, 884, 906
MalI GATC 7 cut(s) 401, 517, 759, 813, 840, 903, 979
MboI GATC 7 cut(s) 399, 515, 757, 811, 838, 901, 977
MboII GAAGA 4 cut(s) 403, 428, 705, 777
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 2 cut(s) 838, 901
MluCI AATT 8 cut(s) 6, 156, 234, 269, 534, 852, 927, 1013
MlyI GAGTC 1 cut(s) 1037
MmeI TCCRAC 3 cut(s) 497, 879, 955
MnlI CCTC 8 cut(s) 31, 370, 537, 784, 787, 790, 793, 820
MseI TTAA 6 cut(s) 17, 159, 339, 375, 717, 791
MslI CAYNNNNRTG 1 cut(s) 202
MspCI CTTAAG 2 cut(s) 716, 790
MspI CCGG 1 cut(s) 895
MunI CAATTG 1 cut(s) 6
MvnI CGCG 1 cut(s) 712
NcoI CCATGG 1 cut(s) 149
NdeII GATC 7 cut(s) 399, 515, 757, 811, 838, 901, 977
NheI GCTAGC 1 cut(s) 110
NlaIII CATG 6 cut(s) 153, 296, 604, 680, 1061, 1115
NlaIV GGNNCC 3 cut(s) 85, 903, 1135
NmuCI GTSAC 3 cut(s) 596, 884, 906
NspI RCATGY 2 cut(s) 296, 680
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PfeI GAWTC 2 cut(s) 452, 922
PinAI ACCGGT 1 cut(s) 894
PkrI GCNGC 1 cut(s) 542
PleI GAGTC 1 cut(s) 1037
PpsI GAGTC 1 cut(s) 1037
Ppu21I YACGTR 1 cut(s) 911
PshBI ATTAAT 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 458
PspN4I GGNNCC 3 cut(s) 85, 903, 1135
PspPI GGNCC 1 cut(s) 225
PstI CTGCAG 2 cut(s) 564, 997
PstNI CAGNNNCTG 1 cut(s) 29
PsuI RGATCY 2 cut(s) 838, 901
RsaI GTAC 3 cut(s) 358, 672, 1075
RsaNI GTAC 3 cut(s) 357, 671, 1074
RseI CAYNNNNRTG 1 cut(s) 202
SalI GTCGAC 1 cut(s) 322
SaqAI TTAA 6 cut(s) 17, 159, 339, 375, 717, 791
SatI GCNGC 1 cut(s) 541
Sau3AI GATC 7 cut(s) 399, 515, 757, 811, 838, 901, 977
Sau96I GGNCC 1 cut(s) 225
ScaI AGTACT 2 cut(s) 672, 1075
SchI GAGTC 1 cut(s) 1037
SfaNI GCATC 2 cut(s) 958, 994
SfcI CTRYAG 2 cut(s) 560, 993
SinI GGWCC 1 cut(s) 225
SmiMI CAYNNNNRTG 1 cut(s) 202
SmlI CTYRAG 3 cut(s) 716, 790, 1045
SmoI CTYRAG 3 cut(s) 716, 790, 1045
Sse9I AATT 8 cut(s) 6, 156, 234, 269, 534, 852, 927, 1013
SspMI CTAG 1 cut(s) 111
StyI CCWWGG 1 cut(s) 149
TaaI ACNGT 4 cut(s) 585, 596, 616, 1027
TaiI ACGT 5 cut(s) 37, 136, 165, 461, 913
TaqI TCGA 9 cut(s) 130, 165, 323, 405, 529, 665, 925, 946, 1039
TasI AATT 8 cut(s) 6, 156, 234, 269, 534, 852, 927, 1013
TatI WGTACW 2 cut(s) 670, 1073
TfiI GAWTC 2 cut(s) 452, 922
Tru1I TTAA 6 cut(s) 17, 159, 339, 375, 717, 791
Tru9I TTAA 6 cut(s) 17, 159, 339, 375, 717, 791
TscAI CASTG 2 cut(s) 61, 1002
TseFI GTSAC 3 cut(s) 596, 884, 906
TseI GCWGC 1 cut(s) 540
Tsp45I GTSAC 3 cut(s) 596, 884, 906
TspGWI ACGGA 4 cut(s) 76, 341, 712, 894
TspRI CASTG 2 cut(s) 61, 1002
Vha464I CTTAAG 2 cut(s) 716, 790
VpaK11BI GGWCC 1 cut(s) 225
VspI ATTAAT 1 cut(s) 339
XapI RAATTY 3 cut(s) 234, 534, 1013
XceI RCATGY 2 cut(s) 296, 680
XmiI GTMKAC 1 cut(s) 323
XspI CTAG 1 cut(s) 111
ZraI GACGTC 1 cut(s) 134
ZrmI AGTACT 2 cut(s) 672, 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.