FvH4_5g08112

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000673
Physical Location & Seq
Reverse (-)
4738181 .. 4739299
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08112.t1

Sequence Viewer

Length: 1119 bp
ATGTTACGTGCAAGCGCCGATCATATAGGTTTATACACAAGGCAAAAATCAAAATCAAAGAAGTATCGTTGGTCTGATCTTCCGTGTGACATGTTACGTGCAATTGCGGAACGACTCCCGTATGTCGATCTCTTTCGCTTCTGCGCCGTGTGCAAGACTTGGAGAGATTGTGCAACAAGTGGGATTCAATACGCCCAAATCCTAATCAGGTACCGTGATTACCAGGGTGAATGTGGCCTGGAGGTCGTTGATTTCTTCACCATGCAGTACCACCGTATCGGAAACAAGATCCGTCGAGGACATCAGCTCTTCGGTTGCTGTCTTCATGCTTCCAAACATGGTTGGTTGCTTTTCTCAAAACCACAAGAAAAACAGCCTTCTGCTACAACAACAACGTTCTTTCTGTACAACCCTCTGGGCAAGACCATCGAGCTACCTTCACTGAACATGGACGGCAAGTTATTTGATAGCAGGAAAGAAGATGTTAAGGTAGTAGCTACCTTTTCGACTAGTGCCATCTTTGTGGTCTCGTACACAAGTTCTATGAGAAAAGATTGCGTTATAAGCACATGTAATCTCAATGATGAAGCAGCAGGATGGATTATTCGAAATTACTCTCGCCCTGAAGATCATGAACTTGAGAACTTAGCCTATATGGATGGAGTTTTCCACTGTGTTTTTAACAAGGGTGAACAGTCATTATTATTTTGTACCTTCAATGTAGAATCTGAAGACTGGGATGTGACACACTATTCCGACTTGTCGAATTTACAATGTTATAATATTTCACGGAGGCCAAGGAAGTATTACTTGGTTGAGTCTGGTGGGGAGCTCTTCTTTGCATTATATCGTCTGGACAGTATTGATTTTACAGTTAGACCAGGCATACACAGCAGCTGGCATATCTACCAGTTTGATAGATCACAGAAAAATTGGACAAGAGTTGAGCTAGGAAACCGAGCATTACTCTTATGTGGCACATCATCCTCCATTCTCATGAGTCTGCATCAGGGGGAAACTAGCAAGAAATTTGCAGGCGAGGTTTTACTTTATGAGTATCCCTGGATGTCCCTCTATCCCTTTTCTTATCCAGACAGGATTGTTCCTTTCTTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

373

Amino Acids

43.29

Weight (kDa)

8.32

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 24 - 60 2.6e-09 F-box domain
F-box-like PF12937 24 - 61 4.2e-07 F-box-like
Beta-prop_KIB1-4 PF03478 105 - 332 1e-16 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 563, 780
Acc65I GGTACC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 210
AciI CCGC 1 cut(s) 107
AclI AACGTT 1 cut(s) 395
AclWI GGATC 1 cut(s) 283
AcsI RAATTY 2 cut(s) 766, 1028
AcuI CTGAAG 2 cut(s) 645, 750
AfaI GTAC 5 cut(s) 212, 269, 407, 533, 712
AflIII ACRYGT 2 cut(s) 90, 569
AgsI TTSAA 2 cut(s) 188, 718
AhlI ACTAGT 1 cut(s) 509
AjnI CCWGG 4 cut(s) 222, 237, 880, 1061
AjuI GAANNNNNNNTTGG 2 cut(s) 794, 826
AluBI AGCT 6 cut(s) 307, 433, 497, 832, 897, 949
AluI AGCT 6 cut(s) 307, 433, 497, 832, 897, 949
Alw21I GWGCWC 1 cut(s) 834
Alw26I GTCTC 1 cut(s) 532
AlwI GGATC 1 cut(s) 283
AlwNI CAGNNNCTG 1 cut(s) 897
AoxI GGCC 2 cut(s) 235, 794
ApeKI GCWGC 2 cut(s) 590, 894
ApoI RAATTY 2 cut(s) 766, 1028
Asp718I GGTACC 1 cut(s) 210
AspLEI GCGC 2 cut(s) 17, 146
AsuHPI GGTGA 3 cut(s) 239, 250, 701
AsuII TTCGAA 1 cut(s) 607
BaeI ACNNNNGTAYC 2 cut(s) 259, 292
BanI GGYRCC 1 cut(s) 210
BanII GRGCYC 1 cut(s) 834
BbsI GAAGAC 2 cut(s) 314, 738
Bbv12I GWGCWC 1 cut(s) 834
BbvI GCAGC 2 cut(s) 602, 906
BccI CCATC 4 cut(s) 434, 524, 591, 653
BceAI ACGGC 2 cut(s) 131, 469
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 4 cut(s) 224, 239, 882, 1063
BciVI GTATCC 1 cut(s) 1068
BcoDI GTCTC 1 cut(s) 532
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 3 cut(s) 510, 950, 1020
BfoI RGCGCY 1 cut(s) 18
BfuI GTATCC 1 cut(s) 1068
BisI GCNGC 2 cut(s) 591, 895
BlsI GCNGC 2 cut(s) 592, 896
Bme1390I CCNGG 4 cut(s) 224, 239, 882, 1063
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 4 cut(s) 224, 239, 882, 1063
BmrI ACTGGG 1 cut(s) 745
BmsI GCATC 1 cut(s) 1015
BmuI ACTGGG 1 cut(s) 745
BpiI GAAGAC 2 cut(s) 314, 738
BplI GAGNNNNNCTC 2 cut(s) 951, 983
BpmI CTGGAG 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 607
BpuEI CTTGAG 1 cut(s) 659
BsaAI YACGTR 2 cut(s) 8, 98
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 3 cut(s) 223, 797, 1061
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bse1I ACTGG 2 cut(s) 740, 910
BseBI CCWGG 4 cut(s) 224, 239, 882, 1063
BseDI CCNNGG 3 cut(s) 223, 797, 1061
BseGI GGATG 5 cut(s) 602, 664, 745, 983, 1071
BseNI ACTGG 2 cut(s) 740, 910
BseXI GCAGC 2 cut(s) 602, 906
BshFI GGCC 2 cut(s) 237, 796
BshNI GGYRCC 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 834
BslFI GGGAC 1 cut(s) 1054
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 1054
BsnI GGCC 2 cut(s) 237, 796
Bso31I GGTCTC 1 cut(s) 532
Bsp119I TTCGAA 1 cut(s) 607
Bsp1286I GDGCHC 1 cut(s) 834
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 6 cut(s) 19, 76, 127, 288, 628, 920
BspACI CCGC 1 cut(s) 107
BspANI GGCC 2 cut(s) 237, 796
BspHI TCATGA 2 cut(s) 631, 996
BspLI GGNNCC 1 cut(s) 212
BspPI GGATC 1 cut(s) 283
BspQI GCTCTTC 2 cut(s) 314, 839
BspT104I TTCGAA 1 cut(s) 607
BspT107I GGYRCC 1 cut(s) 210
BspTNI GGTCTC 1 cut(s) 532
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 740, 910
BssECI CCNNGG 3 cut(s) 223, 797, 1061
BssMI GATC 6 cut(s) 19, 76, 127, 288, 628, 920
BssT1I CCWWGG 1 cut(s) 797
Bst2UI CCWGG 4 cut(s) 224, 239, 882, 1063
Bst4CI ACNGT 6 cut(s) 215, 275, 674, 696, 860, 874
Bst6I CTCTTC 2 cut(s) 314, 839
BstAUI TGTACA 1 cut(s) 405
BstBAI YACGTR 2 cut(s) 8, 98
BstBI TTCGAA 1 cut(s) 607
BstC8I GCNNGC 3 cut(s) 13, 899, 1036
BstDEI CTNAG 1 cut(s) 646
BstF5I GGATG 5 cut(s) 602, 664, 745, 983, 1071
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 2 cut(s) 17, 146
BstKTI GATC 6 cut(s) 22, 79, 130, 291, 631, 923
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 6 cut(s) 19, 76, 127, 288, 628, 920
BstMWI GCNNNNNNNGC 3 cut(s) 150, 564, 891
BstNI CCWGG 4 cut(s) 224, 239, 882, 1063
BstNSI RCATGY 2 cut(s) 94, 573
BstSCI CCNGG 4 cut(s) 222, 237, 880, 1061
BstV1I GCAGC 2 cut(s) 602, 906
BstV2I GAAGAC 2 cut(s) 314, 738
BstX2I RGATCY 1 cut(s) 288
BstXI CCANNNNNNTGG 1 cut(s) 523
BstYI RGATCY 1 cut(s) 288
BsuI GTATCC 1 cut(s) 1068
BsuRI GGCC 2 cut(s) 237, 796
BtsCI GGATG 5 cut(s) 602, 664, 745, 983, 1071
BtsIMutI CAGTG 2 cut(s) 440, 670
Cac8I GCNNGC 3 cut(s) 13, 899, 1036
CaiI CAGNNNCTG 1 cut(s) 897
CciI TCATGA 2 cut(s) 631, 996
CfoI GCGC 2 cut(s) 17, 146
Csp6I GTAC 5 cut(s) 211, 268, 406, 532, 711
CviAII CATG 8 cut(s) 91, 262, 326, 338, 448, 570, 632, 997
CviQI GTAC 5 cut(s) 211, 268, 406, 532, 711
DdeI CTNAG 1 cut(s) 646
DpnI GATC 6 cut(s) 21, 78, 129, 290, 630, 922
DpnII GATC 6 cut(s) 19, 76, 127, 288, 628, 920
Eam1104I CTCTTC 2 cut(s) 314, 839
EarI CTCTTC 2 cut(s) 314, 839
Ecl136II GAGCTC 1 cut(s) 832
Eco130I CCWWGG 1 cut(s) 797
Eco24I GRGCYC 1 cut(s) 834
Eco31I GGTCTC 1 cut(s) 532
Eco53kI GAGCTC 1 cut(s) 832
Eco57I CTGAAG 2 cut(s) 645, 750
EcoICRI GAGCTC 1 cut(s) 832
EcoRII CCWGG 4 cut(s) 222, 237, 880, 1061
EcoT14I CCWWGG 1 cut(s) 797
EcoT38I GRGCYC 1 cut(s) 834
ErhI CCWWGG 1 cut(s) 797
FaeI CATG 8 cut(s) 94, 265, 329, 341, 451, 573, 635, 1000
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FaqI GGGAC 1 cut(s) 1054
FatI CATG 8 cut(s) 90, 261, 325, 337, 447, 569, 631, 996
Fnu4HI GCNGC 2 cut(s) 591, 895
FokI GGATG 5 cut(s) 609, 671, 752, 970, 1078
FriOI GRGCYC 1 cut(s) 834
Fsp4HI GCNGC 2 cut(s) 591, 895
FspBI CTAG 3 cut(s) 510, 950, 1020
GlaI GCGC 2 cut(s) 16, 145
GluI GCNGC 2 cut(s) 591, 895
GsuI CTGGAG 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 2 cut(s) 237, 796
HhaI GCGC 2 cut(s) 17, 146
Hin1II CATG 8 cut(s) 94, 265, 329, 341, 451, 573, 635, 1000
Hin6I GCGC 2 cut(s) 15, 144
HinP1I GCGC 2 cut(s) 15, 144
HinfI GANTC 5 cut(s) 114, 184, 725, 818, 1000
HphI GGTGA 3 cut(s) 239, 250, 701
Hpy166II GTNNAC 2 cut(s) 534, 692
Hpy188I TCNGA 4 cut(s) 76, 281, 730, 757
Hpy188III TCNNGA 4 cut(s) 632, 854, 997, 1091
Hpy8I GTNNAC 2 cut(s) 534, 692
Hpy99I CGWCG 1 cut(s) 297
HpyAV CCTTC 3 cut(s) 387, 447, 724
HpyCH4III ACNGT 6 cut(s) 215, 275, 674, 696, 860, 874
HpyCH4IV ACGT 3 cut(s) 7, 97, 395
HpyCH4V TGCA 8 cut(s) 11, 101, 153, 173, 265, 842, 1006, 1034
HpyF10VI GCNNNNNNNGC 3 cut(s) 150, 564, 891
HpyF3I CTNAG 1 cut(s) 646
HpySE526I ACGT 3 cut(s) 7, 97, 395
Hsp92II CATG 8 cut(s) 94, 265, 329, 341, 451, 573, 635, 1000
HspAI GCGC 2 cut(s) 15, 144
KpnI GGTACC 1 cut(s) 214
Kzo9I GATC 6 cut(s) 19, 76, 127, 288, 628, 920
LguI GCTCTTC 2 cut(s) 314, 839
LmnI GCTCC 1 cut(s) 829
Lsp1109I GCAGC 2 cut(s) 602, 906
LweI GCATC 1 cut(s) 1015
MaeI CTAG 3 cut(s) 510, 950, 1020
MaeII ACGT 3 cut(s) 7, 97, 395
MaeIII GTNAC 4 cut(s) 3, 86, 93, 742
MalI GATC 6 cut(s) 21, 78, 129, 290, 630, 922
MboI GATC 6 cut(s) 19, 76, 127, 288, 628, 920
MboII GAAGA 8 cut(s) 71, 247, 301, 314, 491, 638, 743, 826
MfeI CAATTG 1 cut(s) 102
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 1 cut(s) 834
MluCI AATT 5 cut(s) 102, 610, 766, 931, 1028
MlyI GAGTC 3 cut(s) 108, 827, 1009
MmeI TCCRAC 1 cut(s) 780
MnlI CCTC 7 cut(s) 235, 290, 423, 786, 997, 1033, 1082
MseI TTAA 2 cut(s) 486, 681
MslI CAYNNNNRTG 2 cut(s) 521, 995
MspA1I CMGCKG 1 cut(s) 897
MspR9I CCNGG 4 cut(s) 224, 239, 882, 1063
MunI CAATTG 1 cut(s) 102
MvaI CCWGG 4 cut(s) 224, 239, 882, 1063
MwoI GCNNNNNNNGC 3 cut(s) 150, 564, 891
NdeII GATC 6 cut(s) 19, 76, 127, 288, 628, 920
NlaIII CATG 8 cut(s) 94, 265, 329, 341, 451, 573, 635, 1000
NlaIV GGNNCC 1 cut(s) 212
NmuCI GTSAC 2 cut(s) 86, 742
NspI RCATGY 2 cut(s) 94, 573
NspV TTCGAA 1 cut(s) 607
PagI TCATGA 2 cut(s) 631, 996
PciI ACATGT 2 cut(s) 90, 569
PciSI GCTCTTC 2 cut(s) 314, 839
PfeI GAWTC 2 cut(s) 184, 725
PkrI GCNGC 2 cut(s) 592, 896
PleI GAGTC 3 cut(s) 108, 826, 1008
PpsI GAGTC 3 cut(s) 108, 826, 1008
Ppu21I YACGTR 2 cut(s) 8, 98
PscI ACATGT 2 cut(s) 90, 569
PsiI TTATAA 2 cut(s) 563, 780
Psp124BI GAGCTC 1 cut(s) 834
Psp1406I AACGTT 1 cut(s) 395
Psp6I CCWGG 4 cut(s) 222, 237, 880, 1061
PspGI CCWGG 4 cut(s) 222, 237, 880, 1061
PspN4I GGNNCC 1 cut(s) 212
PstNI CAGNNNCTG 1 cut(s) 897
PsuI RGATCY 1 cut(s) 288
PvuII CAGCTG 1 cut(s) 897
RsaI GTAC 5 cut(s) 212, 269, 407, 533, 712
RsaNI GTAC 5 cut(s) 211, 268, 406, 532, 711
RseI CAYNNNNRTG 2 cut(s) 521, 995
SacI GAGCTC 1 cut(s) 834
SapI GCTCTTC 2 cut(s) 314, 839
SaqAI TTAA 2 cut(s) 486, 681
SatI GCNGC 2 cut(s) 591, 895
Sau3AI GATC 6 cut(s) 19, 76, 127, 288, 628, 920
SchI GAGTC 3 cut(s) 108, 827, 1009
ScrFI CCNGG 4 cut(s) 224, 239, 882, 1063
SduI GDGCHC 1 cut(s) 834
SfaNI GCATC 1 cut(s) 1015
SfuI TTCGAA 1 cut(s) 607
SmiMI CAYNNNNRTG 2 cut(s) 521, 995
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
SpeI ACTAGT 1 cut(s) 509
Sse9I AATT 5 cut(s) 102, 610, 766, 931, 1028
SsiI CCGC 1 cut(s) 107
SspI AATATT 1 cut(s) 784
SspMI CTAG 3 cut(s) 510, 950, 1020
SstI GAGCTC 1 cut(s) 834
StyD4I CCNGG 4 cut(s) 222, 237, 880, 1061
StyI CCWWGG 1 cut(s) 797
TaaI ACNGT 6 cut(s) 215, 275, 674, 696, 860, 874
TaiI ACGT 3 cut(s) 10, 100, 398
TaqI TCGA 6 cut(s) 126, 295, 429, 506, 607, 764
TasI AATT 5 cut(s) 102, 610, 766, 931, 1028
TatI WGTACW 1 cut(s) 405
TfiI GAWTC 2 cut(s) 184, 725
Tru1I TTAA 2 cut(s) 486, 681
Tru9I TTAA 2 cut(s) 486, 681
TscAI CASTG 2 cut(s) 447, 677
TseFI GTSAC 2 cut(s) 86, 742
TseI GCWGC 2 cut(s) 590, 894
Tsp45I GTSAC 2 cut(s) 86, 742
TspDTI ATGAA 3 cut(s) 314, 600, 648
TspGWI ACGGA 3 cut(s) 72, 281, 805
TspRI CASTG 2 cut(s) 447, 677
XapI RAATTY 2 cut(s) 766, 1028
XceI RCATGY 2 cut(s) 94, 573
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 3 cut(s) 510, 950, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.