Rroxscaffold_5G00368090

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000673
Physical Location & Seq
Forward (+)
49839084 .. 49840201
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00368090.1

Sequence Viewer

Length: 660 bp
ATGTCAAATTCATGGTCTGTTCTTCTTCCAGGTGATAACTTCTCCCTAATTGTCGAACATATTTCGTTTAAGGACCTTGTTCGCCTTGGTGTGGTATGCAAGACTTGGAGACCGGTCGTTCTTGAAGCTATTCAACACCATACCATTATATGGAGTACAATTCATACAACTGGTCCTAGTGCTGCTGATATTGGTGATTTGGTCTGTATTGATGGAGTTTTACACTGCCTTTATTATAATGAGAGTGATAGTTCGTTTTTGAAATTGGCTACCTTCAATGTCACACTTCAAGATTGGACTGTGATGAACTATAGATACTCTGAAAGTTCGTTACTGAAACTAGCTGCCTCCGATGCCGCAGTTAATCGGACTCGAAAGAACGATAATTACTTTCATAAATATAACCCTAGGTTTTACTGCTTGGTTGAGTCGACTGATGGGGAGCTGTTCTTAGCAATCAGGTCTACATGTTACAAGTATACTGCTATTAACCAAAGTTATTGGCAAGTTTATCGATTTGGTCTGTCCGAATATGAATGGACTCGAATTACCAGCTTGGGAAATCAAGTATTGTTGATGAATGAGCAGGAATTTCGAGAGGATGGGTGGGAGCAGTGGTCGGCAATGGCAGTTCCAACAGTAGGAGAGGCTAGAGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

25.19

Weight (kDa)

5.49

Isoelectric Point (pI)

27.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 51 - 195 2.3e-07 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 237
AccB7I CCANNNNNTGG 1 cut(s) 150
AccI GTMKAC 3 cut(s) 431, 464, 479
AciI CCGC 1 cut(s) 357
AcsI RAATTY 2 cut(s) 7, 590
AfaI GTAC 1 cut(s) 157
AfiI CCNNNNNNNGG 3 cut(s) 91, 150, 641
AflIII ACRYGT 1 cut(s) 467
AgeI ACCGGT 1 cut(s) 112
AgsI TTSAA 5 cut(s) 125, 134, 262, 277, 290
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 4 cut(s) 128, 344, 445, 555
AluI AGCT 4 cut(s) 128, 344, 445, 555
Alw26I GTCTC 1 cut(s) 103
ApeKI GCWGC 2 cut(s) 182, 344
ApoI RAATTY 2 cut(s) 7, 590
ArsI GACNNNNNNTTYG 1 cut(s) 31
AsiGI ACCGGT 1 cut(s) 112
Asp700I GAANNNNTTC 1 cut(s) 129
AspA2I CCTAGG 1 cut(s) 407
AspS9I GGNCC 2 cut(s) 73, 173
AsuHPI GGTGA 2 cut(s) 44, 206
AvaII GGWCC 2 cut(s) 73, 173
AvrII CCTAGG 1 cut(s) 407
BbvI GCAGC 2 cut(s) 169, 331
BccI CCATC 3 cut(s) 206, 431, 596
BcgI CGANNNNNNTGC 4 cut(s) 494, 528, 575, 609
BciT130I CCWGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 103
BfaI CTAG 4 cut(s) 177, 341, 408, 651
BfmI CTRYAG 1 cut(s) 310
BisI GCNGC 3 cut(s) 183, 345, 357
BlnI CCTAGG 1 cut(s) 407
BlsI GCNGC 3 cut(s) 184, 346, 358
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 2 cut(s) 73, 173
BmgT120I GGNCC 2 cut(s) 73, 173
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 1 cut(s) 343
Bsa29I ATCGAT 1 cut(s) 514
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 2 cut(s) 85, 407
BsaWI WCCGGW 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 150, 641
Bse118I RCCGGY 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 630
BseBI CCWGG 1 cut(s) 30
BseCI ATCGAT 1 cut(s) 514
BseDI CCNNGG 2 cut(s) 85, 407
BseGI GGATG 1 cut(s) 607
BseLI CCNNNNNNNGG 3 cut(s) 91, 150, 641
BseMI GCAATG 1 cut(s) 630
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 2 cut(s) 169, 331
Bsh1285I CGRYCG 1 cut(s) 117
BshTI ACCGGT 1 cut(s) 112
BshVI ATCGAT 1 cut(s) 514
BsiEI CGRYCG 1 cut(s) 117
BsiSI CCGG 1 cut(s) 113
BslI CCNNNNNNNGG 3 cut(s) 91, 150, 641
BsmAI GTCTC 1 cut(s) 103
Bso31I GGTCTC 1 cut(s) 103
BspACI CCGC 1 cut(s) 357
BspDI ATCGAT 1 cut(s) 514
BspTNI GGTCTC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 630
BsrFI RCCGGY 1 cut(s) 112
BsrI ACTGG 1 cut(s) 175
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 85, 407
BssNAI GTATAC 1 cut(s) 480
BssT1I CCWWGG 2 cut(s) 85, 407
Bst1107I GTATAC 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 301, 640
BstDEI CTNAG 1 cut(s) 451
BstF5I GGATG 1 cut(s) 607
BstMAI GTCTC 1 cut(s) 103
BstMCI CGRYCG 1 cut(s) 117
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 1 cut(s) 310
BstV1I GCAGC 2 cut(s) 169, 331
BstZ17I GTATAC 1 cut(s) 480
Bsu15I ATCGAT 1 cut(s) 514
BsuTUI ATCGAT 1 cut(s) 514
BtsCI GGATG 1 cut(s) 607
BtsI GCAGTG 2 cut(s) 223, 620
BtsIMutI CAGTG 2 cut(s) 223, 620
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 73, 173
ClaI ATCGAT 1 cut(s) 514
Csp6I GTAC 1 cut(s) 156
CspAI ACCGGT 1 cut(s) 112
CviAII CATG 2 cut(s) 12, 468
CviJI RGCY 6 cut(s) 128, 269, 344, 445, 555, 650
CviKI_1 RGCY 6 cut(s) 128, 269, 344, 445, 555, 650
CviQI GTAC 1 cut(s) 156
DdeI CTNAG 1 cut(s) 451
Eco130I CCWWGG 2 cut(s) 85, 407
Eco31I GGTCTC 1 cut(s) 103
Eco47I GGWCC 2 cut(s) 73, 173
EcoO109I RGGNCCY 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 2 cut(s) 85, 407
ErhI CCWWGG 2 cut(s) 85, 407
FaeI CATG 2 cut(s) 15, 471
FatI CATG 2 cut(s) 11, 467
FblI GTMKAC 3 cut(s) 431, 464, 479
Fnu4HI GCNGC 3 cut(s) 183, 345, 357
FokI GGATG 1 cut(s) 614
Fsp4HI GCNGC 3 cut(s) 183, 345, 357
FspBI CTAG 4 cut(s) 177, 341, 408, 651
GluI GCNGC 3 cut(s) 183, 345, 357
HapII CCGG 1 cut(s) 113
Hin1II CATG 2 cut(s) 15, 471
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HinfI GANTC 3 cut(s) 370, 428, 541
HpaII CCGG 1 cut(s) 113
HphI GGTGA 2 cut(s) 44, 206
Hpy166II GTNNAC 3 cut(s) 432, 465, 480
Hpy188I TCNGA 4 cut(s) 322, 352, 369, 529
Hpy188III TCNNGA 3 cut(s) 122, 290, 596
Hpy8I GTNNAC 3 cut(s) 432, 465, 480
HpyAV CCTTC 1 cut(s) 283
HpyCH4III ACNGT 2 cut(s) 301, 640
HpyCH4V TGCA 1 cut(s) 99
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 1 cut(s) 451
Hsp92II CATG 2 cut(s) 15, 471
LmnI GCTCC 2 cut(s) 442, 610
LpnPI CCDG 7 cut(s) 15, 42, 126, 156, 445, 565, 572
Lsp1109I GCAGC 2 cut(s) 169, 331
LweI GCATC 1 cut(s) 343
MaeI CTAG 4 cut(s) 177, 341, 408, 651
MaeIII GTNAC 3 cut(s) 280, 330, 470
MboII GAAGA 2 cut(s) 14, 17
MluCI AATT 7 cut(s) 7, 48, 159, 263, 385, 546, 590
MlyI GAGTC 3 cut(s) 364, 437, 535
MmeI TCCRAC 1 cut(s) 659
MnlI CCTC 4 cut(s) 358, 592, 640, 647
MroXI GAANNNNTTC 1 cut(s) 129
MseI TTAA 3 cut(s) 69, 363, 489
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 1 cut(s) 353
NlaIII CATG 2 cut(s) 15, 471
NmuCI GTSAC 1 cut(s) 280
NspI RCATGY 1 cut(s) 471
PciI ACATGT 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 129
PflMI CCANNNNNTGG 1 cut(s) 150
PinAI ACCGGT 1 cut(s) 112
PkrI GCNGC 3 cut(s) 184, 346, 358
PleI GAGTC 3 cut(s) 364, 436, 535
PpsI GAGTC 3 cut(s) 364, 436, 535
PpuMI RGGWCCY 1 cut(s) 73
PscI ACATGT 1 cut(s) 467
PsiI TTATAA 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspPI GGNCC 2 cut(s) 73, 173
PspPPI RGGWCCY 1 cut(s) 73
PsrI GAACNNNNNNTAC 4 cut(s) 299, 331, 371, 403
RsaI GTAC 1 cut(s) 157
RsaNI GTAC 1 cut(s) 156
SalI GTCGAC 1 cut(s) 430
SaqAI TTAA 3 cut(s) 69, 363, 489
SatI GCNGC 3 cut(s) 183, 345, 357
Sau96I GGNCC 2 cut(s) 73, 173
SchI GAGTC 3 cut(s) 364, 437, 535
ScrFI CCNGG 1 cut(s) 30
SetI ASST 9 cut(s) 34, 78, 130, 275, 346, 413, 447, 464, 557
SfaNI GCATC 1 cut(s) 343
SfcI CTRYAG 1 cut(s) 310
SinI GGWCC 2 cut(s) 73, 173
Sse9I AATT 7 cut(s) 7, 48, 159, 263, 385, 546, 590
SsiI CCGC 1 cut(s) 357
SspMI CTAG 4 cut(s) 177, 341, 408, 651
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 2 cut(s) 85, 407
TaaI ACNGT 2 cut(s) 301, 640
TaqI TCGA 6 cut(s) 54, 373, 431, 514, 544, 595
TasI AATT 7 cut(s) 7, 48, 159, 263, 385, 546, 590
TatI WGTACW 1 cut(s) 155
TauI GCSGC 1 cut(s) 359
Tru1I TTAA 3 cut(s) 69, 363, 489
Tru9I TTAA 3 cut(s) 69, 363, 489
TscAI CASTG 2 cut(s) 230, 620
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 2 cut(s) 182, 344
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 5 cut(s) 152, 320, 383, 549, 593
TspRI CASTG 2 cut(s) 230, 620
Van91I CCANNNNNTGG 1 cut(s) 150
VpaK11BI GGWCC 2 cut(s) 73, 173
XapI RAATTY 2 cut(s) 7, 590
XceI RCATGY 1 cut(s) 471
XmaJI CCTAGG 1 cut(s) 407
XmiI GTMKAC 3 cut(s) 431, 464, 479
XmnI GAANNNNTTC 1 cut(s) 129
XspI CTAG 4 cut(s) 177, 341, 408, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.