Rw6G031850

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000673
Physical Location & Seq
Forward (+)
55918958 .. 55920166
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G031850.1

Sequence Viewer

Length: 1209 bp
ATGATTTCTGCACGGGATCGAGCTTGTGTTGAAAAATGTCGAGCTATACCAAGGAAGCTGTTATTGCCCGCTCGAATTTTCTCCTGTTGCCTCGAGCCGAGGACGAGTTCTAAAGAAGAAGAAGAATTGTTGGTCGAAGAAGAAGAAGAAGAAGAAGAAGAAGGATGTTTCAATAATAATAACAACTTGATCCAGAACACAGGCAATGAACATTCTTTTTGGTCCGACCTTCCAATCGATATCTTATGCGAAGTCGCCGATCGTCTCCCATATTGGGATCTTGTTCGTTTTGCTGCCGTATGCAGGACCTGGAGAGTTATCATATATGGGGTTCATCTTCCACAACGCTCCACATTGCTTGTGCCGTACCTGATGAGCTATTCATGTATTCGCGATCGTAAACACATAGAATTCGTAGACCTGCAAGGTGAAACTTACAAATACACAGTTGGGAGAATACTCTTCGACGATACCAGACTCTTTGCTTCAAAATATGGTTGGTTGCTGTTGAAAAAATATGAGCGACCCACAGAACAATTCTATCTCTACAATCCTTTTTGGAATGATGTTATACGACTGCCCCGGTTAGATTTGGAGTTGGATTTTCAGGTAGTATTAGCAACATTCACGACCCCTCCAATCTATGCCGACTGTGTTATCTTCGTCATTTCCAGAAGGTATGGATATACTGATGATCACATCATAAACACATGTAAGCTCCGAGATAGAAGATGGAGTGTTCATAAAACTAGATATCAAGGTTCTGTTGAAGGAGTTACTTACCTAGATGGAATTTTCTATTGTGTTATTCCACAGATAATTAATGGTTCTTTGTCACTTTCGATCAATACCTTCAATATAGCAGTCCAAGACTGGGTCATGGACTATGCCTCCATAACACTAGAACACCATACCCAAAGCACAGCCTTTTGCGTGGTTGAGTCGGATGGGAAATTATTATTAGCAGTTTCTGATGAACTAGTACTTCATGGTACCCGCAATTGGTTTATATACCAGTTTGATTGGTTACGAAAATATTGGACTAGGGTTCAAAGTTTGGGGAATCAGGTATTGCTTCTAAGTCGCTCATCATCAATTGCTTGTCCGACATTGGGGAGGGATAGTAAATTTTCAAACATGATATTTCATCTTAACCTGGGCAAGGAGCCTTTTACTTTCAGTGGTGATGGTGTCTGGAGAAGGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

402

Amino Acids

47.05

Weight (kDa)

5.91

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 74 - 111 4.1e-10 F-box domain
F-box-like PF12937 74 - 110 3.3e-08 F-box-like
Beta-prop_KIB1-4 PF03478 143 - 372 2.3e-24 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 429
Acc65I GGTACC 1 cut(s) 992
AccB1I GGYRCC 1 cut(s) 992
AccBSI CCGCTC 1 cut(s) 71
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 393
AciI CCGC 2 cut(s) 69, 997
AclWI GGATC 3 cut(s) 24, 184, 285
AcsI RAATTY 4 cut(s) 75, 410, 792, 1127
AfaI GTAC 3 cut(s) 368, 984, 994
AfiI CCNNNNNNNGG 6 cut(s) 274, 303, 874, 1002, 1112, 1162
AflIII ACRYGT 1 cut(s) 710
AgsI TTSAA 8 cut(s) 32, 172, 489, 511, 770, 856, 1052, 1134
AhlI ACTAGT 1 cut(s) 979
AjnI CCWGG 2 cut(s) 308, 1155
AluBI AGCT 5 cut(s) 23, 44, 58, 378, 718
AluI AGCT 5 cut(s) 23, 44, 58, 378, 718
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 3 cut(s) 24, 184, 285
AlwNI CAGNNNCTG 2 cut(s) 309, 971
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 1 cut(s) 1202
ApeKI GCWGC 1 cut(s) 293
ApoI RAATTY 4 cut(s) 75, 410, 792, 1127
AseI ATTAAT 1 cut(s) 822
Asp718I GGTACC 1 cut(s) 992
AspS9I GGNCC 3 cut(s) 222, 306, 1203
AsuC2I CCSGG 1 cut(s) 583
AsuHPI GGTGA 2 cut(s) 440, 1196
AvaI CYCGRG 1 cut(s) 92
AvaII GGWCC 2 cut(s) 222, 306
BanI GGYRCC 1 cut(s) 992
BbvI GCAGC 1 cut(s) 280
BccI CCATC 4 cut(s) 726, 782, 941, 1181
BceAI ACGGC 2 cut(s) 281, 349
BciT130I CCWGG 2 cut(s) 310, 1157
BclI TGATCA 1 cut(s) 694
BcnI CCSGG 1 cut(s) 583
BcoDI GTCTC 1 cut(s) 269
BcuI ACTAGT 1 cut(s) 979
BfaI CTAG 6 cut(s) 750, 785, 902, 980, 1044, 1207
BfuAI ACCTGC 1 cut(s) 429
BisI GCNGC 1 cut(s) 294
BlsI GCNGC 1 cut(s) 295
BmcAI AGTACT 1 cut(s) 984
Bme1390I CCNGG 3 cut(s) 310, 583, 1157
Bme18I GGWCC 2 cut(s) 222, 306
BmeT110I CYCGRG 1 cut(s) 92
BmgT120I GGNCC 3 cut(s) 222, 306, 1203
BmiI GGNNCC 2 cut(s) 994, 1167
BmrFI CCNGG 3 cut(s) 310, 583, 1157
BmrI ACTGGG 1 cut(s) 883
BmuI ACTGGG 1 cut(s) 883
BpmI CTGGAG 1 cut(s) 331
BpuMI CCSGG 1 cut(s) 583
Bsa29I ATCGAT 1 cut(s) 237
BsaJI CCNNGG 4 cut(s) 50, 98, 581, 1156
BsaXI ACNNNNNCTCC 6 cut(s) 619, 649, 875, 905, 1108, 1138
Bsc4I CCNNNNNNNGG 6 cut(s) 274, 303, 874, 1002, 1112, 1162
Bse1I ACTGG 2 cut(s) 878, 1015
Bse3DI GCAATG 2 cut(s) 211, 353
BseBI CCWGG 2 cut(s) 310, 1157
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 4 cut(s) 50, 98, 581, 1156
BseGI GGATG 2 cut(s) 170, 952
BseLI CCNNNNNNNGG 6 cut(s) 274, 303, 874, 1002, 1112, 1162
BseMI GCAATG 2 cut(s) 211, 353
BseNI ACTGG 2 cut(s) 878, 1015
BseXI GCAGC 1 cut(s) 280
Bsh1236I CGCG 1 cut(s) 393
Bsh1285I CGRYCG 2 cut(s) 262, 397
BshFI GGCC 1 cut(s) 1204
BshNI GGYRCC 1 cut(s) 992
BshVI ATCGAT 1 cut(s) 237
BsiEI CGRYCG 2 cut(s) 262, 397
BsiHKCI CYCGRG 1 cut(s) 92
BsiSI CCGG 1 cut(s) 583
BslI CCNNNNNNNGG 6 cut(s) 274, 303, 874, 1002, 1112, 1162
BsmAI GTCTC 1 cut(s) 269
BsmBI CGTCTC 1 cut(s) 269
BsnI GGCC 1 cut(s) 1204
BsoBI CYCGRG 1 cut(s) 92
Bsp143I GATC 7 cut(s) 16, 189, 259, 277, 394, 694, 843
Bsp68I TCGCGA 1 cut(s) 393
BspACI CCGC 2 cut(s) 69, 997
BspANI GGCC 1 cut(s) 1204
BspDI ATCGAT 1 cut(s) 237
BspFNI CGCG 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 994, 1167
BspMI ACCTGC 1 cut(s) 429
BspPI GGATC 3 cut(s) 24, 184, 285
BspT107I GGYRCC 1 cut(s) 992
BsrBI CCGCTC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 211, 353
BsrI ACTGG 2 cut(s) 878, 1015
BssECI CCNNGG 4 cut(s) 50, 98, 581, 1156
BssMI GATC 7 cut(s) 16, 189, 259, 277, 394, 694, 843
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 310, 1157
Bst4CI ACNGT 2 cut(s) 448, 653
Bst6I CTCTTC 1 cut(s) 467
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 1079
BstENI CCTNNNNNAGG 1 cut(s) 1160
BstF5I GGATG 2 cut(s) 170, 952
BstFNI CGCG 1 cut(s) 393
BstKTI GATC 7 cut(s) 19, 192, 262, 280, 397, 697, 846
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 7 cut(s) 16, 189, 259, 277, 394, 694, 843
BstMCI CGRYCG 2 cut(s) 262, 397
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNI CCWGG 2 cut(s) 310, 1157
BstNSI RCATGY 1 cut(s) 714
BstSCI CCNGG 3 cut(s) 308, 581, 1155
BstUI CGCG 1 cut(s) 393
BstV1I GCAGC 1 cut(s) 280
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 237
BsuRI GGCC 1 cut(s) 1204
BsuTUI ATCGAT 1 cut(s) 237
BtsCI GGATG 2 cut(s) 170, 952
BtsIMutI CAGTG 1 cut(s) 1186
BtuMI TCGCGA 1 cut(s) 393
BveI ACCTGC 1 cut(s) 429
Cac8I GCNNGC 1 cut(s) 69
CaiI CAGNNNCTG 2 cut(s) 309, 971
Cfr13I GGNCC 3 cut(s) 222, 306, 1203
ClaI ATCGAT 1 cut(s) 237
Csp6I GTAC 3 cut(s) 367, 983, 993
CspCI CAANNNNNGTGG 2 cut(s) 340, 375
CviAII CATG 5 cut(s) 384, 711, 880, 989, 1138
CviJI RGCY 9 cut(s) 23, 44, 58, 97, 378, 718, 926, 1168, 1204
CviKI_1 RGCY 9 cut(s) 23, 44, 58, 97, 378, 718, 926, 1168, 1204
CviQI GTAC 3 cut(s) 367, 983, 993
DdeI CTNAG 1 cut(s) 1079
DpnI GATC 7 cut(s) 18, 191, 261, 279, 396, 696, 845
DpnII GATC 7 cut(s) 16, 189, 259, 277, 394, 694, 843
Eam1104I CTCTTC 1 cut(s) 467
EarI CTCTTC 1 cut(s) 467
Eco130I CCWWGG 1 cut(s) 50
Eco32I GATATC 2 cut(s) 241, 755
Eco47I GGWCC 2 cut(s) 222, 306
Eco88I CYCGRG 1 cut(s) 92
EcoNI CCTNNNNNAGG 1 cut(s) 1160
EcoO109I RGGNCCY 2 cut(s) 306, 1203
EcoRI GAATTC 1 cut(s) 410
EcoRII CCWGG 2 cut(s) 308, 1155
EcoRV GATATC 2 cut(s) 241, 755
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 5 cut(s) 387, 714, 883, 992, 1141
FatI CATG 5 cut(s) 383, 710, 879, 988, 1137
FauI CCCGC 2 cut(s) 76, 1004
FbaI TGATCA 1 cut(s) 694
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 1 cut(s) 294
FokI GGATG 2 cut(s) 177, 959
Fsp4HI GCNGC 1 cut(s) 294
FspBI CTAG 6 cut(s) 750, 785, 902, 980, 1044, 1207
GluI GCNGC 1 cut(s) 294
GsuI CTGGAG 1 cut(s) 331
HaeIII GGCC 1 cut(s) 1204
HapII CCGG 1 cut(s) 583
Hin1II CATG 5 cut(s) 387, 714, 883, 992, 1141
HinfI GANTC 3 cut(s) 477, 941, 1063
HpaII CCGG 1 cut(s) 583
HphI GGTGA 2 cut(s) 440, 1196
Hpy166II GTNNAC 2 cut(s) 401, 418
Hpy188I TCNGA 5 cut(s) 226, 722, 946, 973, 1107
Hpy188III TCNNGA 5 cut(s) 193, 392, 628, 672, 1195
Hpy8I GTNNAC 2 cut(s) 401, 418
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 6 cut(s) 155, 239, 669, 764, 862, 1194
HpyCH4III ACNGT 2 cut(s) 448, 653
HpyCH4V TGCA 3 cut(s) 11, 303, 424
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpyF3I CTNAG 1 cut(s) 1079
Hsp92II CATG 5 cut(s) 387, 714, 883, 992, 1141
KpnI GGTACC 1 cut(s) 996
Ksp22I TGATCA 1 cut(s) 694
Kzo9I GATC 7 cut(s) 16, 189, 259, 277, 394, 694, 843
LmnI GCTCC 3 cut(s) 353, 723, 1165
Lsp1109I GCAGC 1 cut(s) 280
MaeI CTAG 6 cut(s) 750, 785, 902, 980, 1044, 1207
MaeIII GTNAC 3 cut(s) 775, 834, 1026
MalI GATC 7 cut(s) 18, 191, 261, 279, 396, 696, 845
MbiI CCGCTC 1 cut(s) 71
MboI GATC 7 cut(s) 16, 189, 259, 277, 394, 694, 843
MfeI CAATTG 2 cut(s) 1000, 1095
MflI RGATCY 1 cut(s) 277
MlyI GAGTC 2 cut(s) 471, 950
MmeI TCCRAC 4 cut(s) 249, 579, 924, 1130
MnlI CCTC 5 cut(s) 93, 101, 645, 901, 1110
MseI TTAA 2 cut(s) 822, 1152
MspI CCGG 1 cut(s) 583
MspR9I CCNGG 3 cut(s) 310, 583, 1157
MunI CAATTG 2 cut(s) 1000, 1095
MvaI CCWGG 2 cut(s) 310, 1157
MvnI CGCG 1 cut(s) 393
MwoI GCNNNNNNNGC 1 cut(s) 64
NciI CCSGG 1 cut(s) 583
NdeII GATC 7 cut(s) 16, 189, 259, 277, 394, 694, 843
NlaIII CATG 5 cut(s) 387, 714, 883, 992, 1141
NlaIV GGNNCC 2 cut(s) 994, 1167
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 834
NruI TCGCGA 1 cut(s) 393
NspI RCATGY 1 cut(s) 714
PaeR7I CTCGAG 1 cut(s) 92
PciI ACATGT 1 cut(s) 710
PfeI GAWTC 1 cut(s) 1063
PflFI GACNNNGTC 1 cut(s) 875
PkrI GCNGC 1 cut(s) 295
Ple19I CGATCG 2 cut(s) 262, 397
PleI GAGTC 2 cut(s) 471, 949
PpsI GAGTC 2 cut(s) 471, 949
PpuMI RGGWCCY 1 cut(s) 306
PscI ACATGT 1 cut(s) 710
PshBI ATTAAT 1 cut(s) 822
Psp5II RGGWCCY 1 cut(s) 306
Psp6I CCWGG 2 cut(s) 308, 1155
PspGI CCWGG 2 cut(s) 308, 1155
PspN4I GGNNCC 2 cut(s) 994, 1167
PspPI GGNCC 3 cut(s) 222, 306, 1203
PspPPI RGGWCCY 1 cut(s) 306
PspXI VCTCGAGB 1 cut(s) 92
PstNI CAGNNNCTG 2 cut(s) 309, 971
PsuI RGATCY 1 cut(s) 277
PsyI GACNNNGTC 1 cut(s) 875
PvuI CGATCG 2 cut(s) 262, 397
RruI TCGCGA 1 cut(s) 393
RsaI GTAC 3 cut(s) 368, 984, 994
RsaNI GTAC 3 cut(s) 367, 983, 993
SaqAI TTAA 2 cut(s) 822, 1152
SatI GCNGC 1 cut(s) 294
Sau3AI GATC 7 cut(s) 16, 189, 259, 277, 394, 694, 843
Sau96I GGNCC 3 cut(s) 222, 306, 1203
ScaI AGTACT 1 cut(s) 984
SchI GAGTC 2 cut(s) 471, 950
ScrFI CCNGG 3 cut(s) 310, 583, 1157
Sfr274I CTCGAG 1 cut(s) 92
SinI GGWCC 2 cut(s) 222, 306
SlaI CTCGAG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SpeI ACTAGT 1 cut(s) 979
SsiI CCGC 2 cut(s) 69, 997
SspI AATATT 1 cut(s) 1037
SspMI CTAG 6 cut(s) 750, 785, 902, 980, 1044, 1207
StyD4I CCNGG 3 cut(s) 308, 581, 1155
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 448, 653
TaqI TCGA 8 cut(s) 19, 40, 73, 93, 135, 237, 465, 842
TatI WGTACW 1 cut(s) 982
TfiI GAWTC 1 cut(s) 1063
Tru1I TTAA 2 cut(s) 822, 1152
Tru9I TTAA 2 cut(s) 822, 1152
TscAI CASTG 1 cut(s) 1186
TseFI GTSAC 1 cut(s) 834
TseI GCWGC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 834
TspDTI ATGAA 7 cut(s) 222, 323, 372, 731, 977, 990, 1136
TspRI CASTG 1 cut(s) 1186
Tth111I GACNNNGTC 1 cut(s) 875
VpaK11BI GGWCC 2 cut(s) 222, 306
VspI ATTAAT 1 cut(s) 822
XagI CCTNNNNNAGG 1 cut(s) 1160
XapI RAATTY 4 cut(s) 75, 410, 792, 1127
XceI RCATGY 1 cut(s) 714
XhoI CTCGAG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 417
XspI CTAG 6 cut(s) 750, 785, 902, 980, 1044, 1207
ZrmI AGTACT 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.