Rmu_sc0001576.1_g000022

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001576.1
OG0000673
Physical Location & Seq
Forward (+)
91310 .. 93203
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001576.1_g000022.1.cds

Sequence Viewer

Length: 1344 bp
atgtctatggaaaacaactattcttgttggtctaatatttcgtttgacatcttaagttcagtagccgaacgcctcccttatcgtgatctctttcgcatcggcgcagtgtgcaggagttggagagcttgtatgattcgtgggattcaatccacagcgataattaggtactgttcttacgagcagaaatatgcactggagctcgtggacctcttgcacatgcaatttcactgtatcggaaacatgaatatccttcaaggacatcagctctttggttgccgtcttcatgcttcgaaacatggttggttacttttctcaaaaccacaacaagaacagcctgcttcaactacaacaaaacatggtttgttacttttcccaaaaccacaacaagaacagcgttcaactagaacattctttttctacaaccctctgggcaagactatggaactaccctttttgaacatggacatgtttgataatgacgaagatgttaaggtagcagctatcttttcgactagtccaacttgtcatgactgtgtaatctttgtgatcctctatagaagtaccgatggtcgatgttctacaagcaaacattgctttataagcacctgtaaactcagtgataaaagatggattactcgagagtatcctcggcctcaagatcatgaaattgggggcttagcttatgtggatggagttgtccactgtgttttttacctgggatactcgtcaataacatttgctaccttccatgtagcccttaaagactgggttatgatagactattccgccttttcaaaactacttgttttctttccatggaagtgttacttggttgagtctggtagggagctcttatttgcattttacggtctggacaactcttttgataaggatagagagattctttactggcatatctaccaatttgatcgctcaaggaaagattggactagagttttaacgttagggaaccgagcattactcatatatggcgtatcatcctccattatcataagtgtggcagaggggaaaactggggaaattgcagacaaggagagcaccaacctcatcacaaggttcttttctgtgccttttggaaaaggacttctgcctttggttattaccatgcctcctatcaccactctcttccccgccggaactctcaccatcatcacggccttgagtttcgatgccagcaaggcgttcactaagttgttcaatcaccccttgatttcctccatgtcgttggtattgccacgaccctccatccgaaaacctctcctaccacgtctgttagccatggaacccgtctatcgatcccaccaaacagaatctgggctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

447

Amino Acids

51.26

Weight (kDa)

8.77

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 599
AciI CCGC 2 cut(s) 786, 1152
AclI AACGTT 1 cut(s) 962
AclWI GGATC 2 cut(s) 541, 1311
AfaI GTAC 2 cut(s) 167, 562
AflII CTTAAG 1 cut(s) 52
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 7 cut(s) 146, 254, 342, 399, 457, 795, 1219
AhlI ACTAGT 1 cut(s) 512
AjiI CACGTC 1 cut(s) 1289
AjnI CCWGG 1 cut(s) 714
AjuI GAANNNNNNNTTGG 2 cut(s) 812, 844
AluBI AGCT 6 cut(s) 125, 199, 265, 500, 680, 850
AluI AGCT 6 cut(s) 125, 199, 265, 500, 680, 850
Alw21I GWGCWC 3 cut(s) 201, 852, 1061
AlwI GGATC 2 cut(s) 541, 1311
AlwNI CAGNNNCTG 1 cut(s) 1334
Ama87I CYCGRG 1 cut(s) 636
AoxI GGCC 2 cut(s) 650, 1176
ApeKI GCWGC 1 cut(s) 497
ArsI GACNNNNNNTTYG 2 cut(s) 866, 898
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 3 cut(s) 1129, 1156, 1214
AsuII TTCGAA 1 cut(s) 290
AvaI CYCGRG 1 cut(s) 636
AvaII GGWCC 1 cut(s) 205
BanII GRGCYC 3 cut(s) 201, 852, 1341
BauI CACGAG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 272
Bbv12I GWGCWC 3 cut(s) 201, 852, 1061
BbvI GCAGC 1 cut(s) 509
BccI CCATC 5 cut(s) 560, 621, 683, 1175, 1274
BceAI ACGGC 2 cut(s) 261, 1191
BciT130I CCWGG 1 cut(s) 716
BciVI GTATCC 2 cut(s) 654, 713
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 3 cut(s) 402, 513, 951
BfmI CTRYAG 1 cut(s) 553
BfrI CTTAAG 1 cut(s) 52
BfuI GTATCC 2 cut(s) 654, 713
BglI GCCNNNNNGGC 1 cut(s) 1199
BisI GCNGC 1 cut(s) 498
BlpI GCTNAGC 1 cut(s) 676
BlsI GCNGC 1 cut(s) 499
Bme1390I CCNGG 1 cut(s) 716
Bme18I GGWCC 1 cut(s) 205
BmeT110I CYCGRG 1 cut(s) 636
BmgBI CACGTC 1 cut(s) 1289
BmgT120I GGNCC 1 cut(s) 205
BmiI GGNNCC 2 cut(s) 971, 1305
BmrFI CCNGG 1 cut(s) 716
BmrI ACTGGG 2 cut(s) 775, 1044
BmsI GCATC 2 cut(s) 105, 1180
BmuI ACTGGG 2 cut(s) 775, 1044
BpiI GAAGAC 1 cut(s) 272
BplI GAGNNNNNCTC 2 cut(s) 966, 998
BpmI CTGGAG 1 cut(s) 215
Bpu1102I GCTNAGC 1 cut(s) 676
Bpu14I TTCGAA 1 cut(s) 290
BpuEI CTTGAG 3 cut(s) 639, 919, 1201
Bsa29I ATCGAT 1 cut(s) 1315
BsaJI CCNNGG 4 cut(s) 647, 715, 815, 1299
BsaXI ACNNNNNCTCC 4 cut(s) 112, 142, 1114, 1144
Bse1I ACTGG 4 cut(s) 198, 770, 914, 1039
Bse3DI GCAATG 1 cut(s) 589
BseBI CCWGG 1 cut(s) 716
BseCI ATCGAT 1 cut(s) 1315
BseDI CCNNGG 4 cut(s) 647, 715, 815, 1299
BseGI GGATG 3 cut(s) 694, 998, 1266
BseMI GCAATG 1 cut(s) 589
BseMII CTCAG 1 cut(s) 628
BseNI ACTGG 4 cut(s) 198, 770, 914, 1039
BseXI GCAGC 1 cut(s) 509
BsgI GTGCAG 1 cut(s) 130
BshFI GGCC 2 cut(s) 652, 1178
BshVI ATCGAT 1 cut(s) 1315
BsiHKAI GWGCWC 3 cut(s) 201, 852, 1061
BsiHKCI CYCGRG 1 cut(s) 636
BsiSI CCGG 1 cut(s) 1155
BsnI GGCC 2 cut(s) 652, 1178
BsoBI CYCGRG 1 cut(s) 636
Bsp119I TTCGAA 1 cut(s) 290
Bsp1286I GDGCHC 4 cut(s) 201, 852, 1061, 1341
Bsp143I GATC 5 cut(s) 85, 546, 658, 928, 1316
Bsp1720I GCTNAGC 1 cut(s) 676
Bsp19I CCATGG 2 cut(s) 815, 1299
BspACI CCGC 2 cut(s) 786, 1152
BspANI GGCC 2 cut(s) 652, 1178
BspCNI CTCAG 1 cut(s) 627
BspDI ATCGAT 1 cut(s) 1315
BspHI TCATGA 2 cut(s) 526, 661
BspLI GGNNCC 2 cut(s) 971, 1305
BspPI GGATC 2 cut(s) 541, 1311
BspT104I TTCGAA 1 cut(s) 290
BspTI CTTAAG 1 cut(s) 52
BsrDI GCAATG 1 cut(s) 589
BsrI ACTGG 4 cut(s) 198, 770, 914, 1039
BssECI CCNNGG 4 cut(s) 647, 715, 815, 1299
BssMI GATC 5 cut(s) 85, 546, 658, 928, 1316
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 2 cut(s) 815, 1299
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 716
Bst4CI ACNGT 5 cut(s) 170, 230, 533, 704, 869
Bst6I CTCTTC 1 cut(s) 1151
BstAFI CTTAAG 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 591
BstBI TTCGAA 1 cut(s) 290
BstC8I GCNNGC 2 cut(s) 336, 1195
BstDEI CTNAG 3 cut(s) 614, 676, 1209
BstDSI CCRYGG 2 cut(s) 815, 1299
BstF5I GGATG 3 cut(s) 694, 998, 1266
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 5 cut(s) 88, 549, 661, 931, 1319
BstMBI GATC 5 cut(s) 85, 546, 658, 928, 1316
BstMWI GCNNNNNNNGC 4 cut(s) 108, 591, 600, 1199
BstNI CCWGG 1 cut(s) 716
BstNSI RCATGY 2 cut(s) 220, 469
BstSCI CCNGG 1 cut(s) 714
BstSFI CTRYAG 1 cut(s) 553
BstV1I GCAGC 1 cut(s) 509
BstV2I GAAGAC 1 cut(s) 272
BstXI CCANNNNNNTGG 1 cut(s) 1246
Bsu15I ATCGAT 1 cut(s) 1315
BsuI GTATCC 2 cut(s) 654, 713
BsuRI GGCC 2 cut(s) 652, 1178
BsuTUI ATCGAT 1 cut(s) 1315
BtgI CCRYGG 2 cut(s) 815, 1299
BtrI CACGTC 1 cut(s) 1289
BtsCI GGATG 3 cut(s) 694, 998, 1266
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 5 cut(s) 111, 191, 226, 622, 700
Cac8I GCNNGC 2 cut(s) 336, 1195
CaiI CAGNNNCTG 1 cut(s) 1334
CciI TCATGA 2 cut(s) 526, 661
CfoI GCGC 1 cut(s) 104
Cfr13I GGNCC 1 cut(s) 205
ClaI ATCGAT 1 cut(s) 1315
Csp6I GTAC 2 cut(s) 166, 561
CviQI GTAC 2 cut(s) 166, 561
DdeI CTNAG 3 cut(s) 614, 676, 1209
DpnI GATC 5 cut(s) 87, 548, 660, 930, 1318
DpnII GATC 5 cut(s) 85, 546, 658, 928, 1316
Eam1104I CTCTTC 1 cut(s) 1151
EarI CTCTTC 1 cut(s) 1151
EciI GGCGGA 1 cut(s) 775
Ecl136II GAGCTC 2 cut(s) 199, 850
Eco130I CCWWGG 2 cut(s) 815, 1299
Eco24I GRGCYC 3 cut(s) 201, 852, 1341
Eco47I GGWCC 1 cut(s) 205
Eco53kI GAGCTC 2 cut(s) 199, 850
Eco88I CYCGRG 1 cut(s) 636
EcoICRI GAGCTC 2 cut(s) 199, 850
EcoRII CCWGG 1 cut(s) 714
EcoT14I CCWWGG 2 cut(s) 815, 1299
EcoT38I GRGCYC 3 cut(s) 201, 852, 1341
ErhI CCWWGG 2 cut(s) 815, 1299
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FauI CCCGC 1 cut(s) 1159
Fnu4HI GCNGC 1 cut(s) 498
FokI GGATG 3 cut(s) 701, 985, 1253
FriOI GRGCYC 3 cut(s) 201, 852, 1341
Fsp4HI GCNGC 1 cut(s) 498
FspBI CTAG 3 cut(s) 402, 513, 951
GlaI GCGC 1 cut(s) 103
GluI GCNGC 1 cut(s) 498
GsuI CTGGAG 1 cut(s) 215
HaeIII GGCC 2 cut(s) 652, 1178
HapII CCGG 1 cut(s) 1155
HhaI GCGC 1 cut(s) 104
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HinfI GANTC 5 cut(s) 133, 142, 836, 901, 1331
HpaII CCGG 1 cut(s) 1155
HphI GGTGA 3 cut(s) 1129, 1156, 1214
Hpy166II GTNNAC 4 cut(s) 205, 611, 700, 1206
Hpy188I TCNGA 3 cut(s) 236, 1271, 1343
Hpy188III TCNNGA 6 cut(s) 83, 527, 638, 656, 662, 872
Hpy8I GTNNAC 4 cut(s) 205, 611, 700, 1206
HpyAV CCTTC 2 cut(s) 260, 754
HpyCH4III ACNGT 5 cut(s) 170, 230, 533, 704, 869
HpyCH4IV ACGT 2 cut(s) 962, 1288
HpyCH4V TGCA 6 cut(s) 111, 191, 214, 220, 860, 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 591, 600, 1199
HpyF3I CTNAG 3 cut(s) 614, 676, 1209
HpySE526I ACGT 2 cut(s) 962, 1288
HspAI GCGC 1 cut(s) 102
Kzo9I GATC 5 cut(s) 85, 546, 658, 928, 1316
LmnI GCTCC 2 cut(s) 196, 847
Lsp1109I GCAGC 1 cut(s) 509
LweI GCATC 2 cut(s) 105, 1180
MaeI CTAG 3 cut(s) 402, 513, 951
MaeII ACGT 2 cut(s) 962, 1288
MaeIII GTNAC 3 cut(s) 303, 363, 824
MalI GATC 5 cut(s) 87, 548, 660, 930, 1318
MboI GATC 5 cut(s) 85, 546, 658, 928, 1316
MboII GAAGA 3 cut(s) 272, 494, 1138
MhlI GDGCHC 4 cut(s) 201, 852, 1061, 1341
MluCI AATT 5 cut(s) 159, 221, 666, 923, 1041
MlyI GAGTC 1 cut(s) 845
MmeI TCCRAC 2 cut(s) 98, 542
MseI TTAA 4 cut(s) 53, 489, 759, 959
MslI CAYNNNNRTG 4 cut(s) 464, 531, 820, 1016
MspCI CTTAAG 1 cut(s) 52
MspI CCGG 1 cut(s) 1155
MspR9I CCNGG 1 cut(s) 716
MvaI CCWGG 1 cut(s) 716
MwoI GCNNNNNNNGC 4 cut(s) 108, 591, 600, 1199
NcoI CCATGG 2 cut(s) 815, 1299
NdeII GATC 5 cut(s) 85, 546, 658, 928, 1316
NlaIV GGNNCC 2 cut(s) 971, 1305
NmeAIII GCCGAG 1 cut(s) 628
NspI RCATGY 2 cut(s) 220, 469
NspV TTCGAA 1 cut(s) 290
PaeR7I CTCGAG 1 cut(s) 636
PagI TCATGA 2 cut(s) 526, 661
PciI ACATGT 1 cut(s) 465
PfeI GAWTC 4 cut(s) 133, 142, 901, 1331
PkrI GCNGC 1 cut(s) 499
PleI GAGTC 1 cut(s) 844
PpsI GAGTC 1 cut(s) 844
PscI ACATGT 1 cut(s) 465
PsiI TTATAA 1 cut(s) 599
Psp124BI GAGCTC 2 cut(s) 201, 852
Psp1406I AACGTT 1 cut(s) 962
Psp6I CCWGG 1 cut(s) 714
PspGI CCWGG 1 cut(s) 714
PspN4I GGNNCC 2 cut(s) 971, 1305
PspPI GGNCC 1 cut(s) 205
PstNI CAGNNNCTG 1 cut(s) 1334
RsaI GTAC 2 cut(s) 167, 562
RsaNI GTAC 2 cut(s) 166, 561
RseI CAYNNNNRTG 4 cut(s) 464, 531, 820, 1016
SacI GAGCTC 2 cut(s) 201, 852
SaqAI TTAA 4 cut(s) 53, 489, 759, 959
SatI GCNGC 1 cut(s) 498
Sau3AI GATC 5 cut(s) 85, 546, 658, 928, 1316
Sau96I GGNCC 1 cut(s) 205
SchI GAGTC 1 cut(s) 845
ScrFI CCNGG 1 cut(s) 716
SduI GDGCHC 4 cut(s) 201, 852, 1061, 1341
SfaNI GCATC 2 cut(s) 105, 1180
SfcI CTRYAG 1 cut(s) 553
Sfr274I CTCGAG 1 cut(s) 636
SfuI TTCGAA 1 cut(s) 290
SinI GGWCC 1 cut(s) 205
SlaI CTCGAG 1 cut(s) 636
SmiMI CAYNNNNRTG 4 cut(s) 464, 531, 820, 1016
SmlI CTYRAG 5 cut(s) 52, 636, 654, 934, 1180
SmoI CTYRAG 5 cut(s) 52, 636, 654, 934, 1180
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 5 cut(s) 159, 221, 666, 923, 1041
SsiI CCGC 2 cut(s) 786, 1152
SspI AATATT 1 cut(s) 37
SspMI CTAG 3 cut(s) 402, 513, 951
SstI GAGCTC 2 cut(s) 201, 852
StyD4I CCNGG 1 cut(s) 714
StyI CCWWGG 2 cut(s) 815, 1299
TaaI ACNGT 5 cut(s) 170, 230, 533, 704, 869
TaiI ACGT 2 cut(s) 965, 1291
TaqI TCGA 6 cut(s) 290, 509, 571, 637, 1188, 1315
TasI AATT 5 cut(s) 159, 221, 666, 923, 1041
TfiI GAWTC 4 cut(s) 133, 142, 901, 1331
Tru1I TTAA 4 cut(s) 53, 489, 759, 959
Tru9I TTAA 4 cut(s) 53, 489, 759, 959
TscAI CASTG 5 cut(s) 111, 198, 233, 622, 707
TseI GCWGC 1 cut(s) 497
TspDTI ATGAA 3 cut(s) 257, 272, 678
TspRI CASTG 5 cut(s) 111, 198, 233, 622, 707
Vha464I CTTAAG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 205
XceI RCATGY 2 cut(s) 220, 469
XcmI CCANNNNNNNNNTGG 1 cut(s) 1331
XhoI CTCGAG 1 cut(s) 636
XspI CTAG 3 cut(s) 402, 513, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.