Rh5AG254200

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000673
Physical Location & Seq
Forward (+)
32759480 .. 32760610
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG254200.1

Sequence Viewer

Length: 981 bp
ATGATGAAAAATTCTAACAGAAAGAAAAACTCGTGTGATCAAACTCGAGCAGATCGTATCACTCCAACCCGTTCAAACCATCGTTGTTGGTCTGATCTTCCAGTTGACCTGTCAAGATCAGTGGTTGAGCGTCTCATATATCCTGATTTTCTTCGCTTTCGTGCCGTATACAAAAACTGGCAGCGAGCTTTTTCCGACGACGATGACCTTCAGCGACGGGCAGTATTTCCTTTAATACGGGTTGACAGTTCTCAACTTCTCATTACTTCCATTATACCAATACCCAAACCGTTTGTACAGCGCGCTTCTTTGATTTGCTGTCCCCATTACAAGTCTTCCCTATCAGAAACATGCGAGACTGTGGAATTGCATCTACCTCCATCAGTTTCGCGGCACTACCTTTCCAGAGAGGTAGAGGTAGCAGGCTTCTCGACCACTCCAACTTCTCTGGATTGTGTTATCTTTCTCATGTGGAAAATTCATAAATGCAATGGTTATGATTCTGATCCTAATTACGAGGAGTGGAATGAGTTCTTTATAACCACGTACAACCAAAAGTATAGAAGTTGGAGTGTGCATGAACTTCCTCGTCGCAATCCCAAAAATAAGGAGTGGAAGTTCATCAAGGACTTCAAGAGGGGTTGGAGTTTACATGGATGGAAGTTTCTATTGTGCTGTGTCTATGAATTCAAAGGTTATATTGGGTACCTTAAATGTAGCACTTCAAGAGGGGTTATGATGATGAAGAGATCCTTGAGACGACCATCTCGTCCAGAACTCATGAACTTGGTTAAATCTGATGAAGGGCTAGTATTGGCAGTTTGTTTTAAGGAGCAAGATGGTGATGAATTTTTCTACCATATTTACCAGTTTGATTTGTCACGAAAAGAATGGACAAGAGTTAAAAGTTTGGGAAACCAAGTATTGATGTTGCATAGCCGTCCGTGGCTTGGACTCGGACAGCTATGTTGCTTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

326

Amino Acids

38.7

Weight (kDa)

9.26

Isoelectric Point (pI)

44.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
AasI GACNNNNNNGTC 1 cut(s) 768
Acc65I GGTACC 1 cut(s) 705
AccB1I GGYRCC 1 cut(s) 705
AccI GTMKAC 1 cut(s) 168
AccII CGCG 2 cut(s) 303, 391
AciI CCGC 1 cut(s) 391
AclWI GGATC 2 cut(s) 500, 744
AcsI RAATTY 4 cut(s) 10, 477, 686, 848
AcuI CTGAAG 1 cut(s) 194
AfaI GTAC 3 cut(s) 297, 548, 707
AfiI CCNNNNNNNGG 1 cut(s) 950
AgsI TTSAA 4 cut(s) 75, 634, 691, 726
AloI GAACNNNNNNTCC 2 cut(s) 602, 634
AluBI AGCT 2 cut(s) 188, 964
AluI AGCT 2 cut(s) 188, 964
Alw26I GTCTC 3 cut(s) 137, 350, 751
AlwI GGATC 2 cut(s) 500, 744
Ama87I CYCGRG 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 181
ApoI RAATTY 4 cut(s) 10, 477, 686, 848
Asp700I GAANNNNTTC 1 cut(s) 530
Asp718I GGTACC 1 cut(s) 705
AspLEI GCGC 2 cut(s) 303, 305
AsuHPI GGTGA 1 cut(s) 854
AvaI CYCGRG 1 cut(s) 45
BanI GGYRCC 1 cut(s) 705
BauI CACGAG 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 193
BccI CCATC 5 cut(s) 87, 388, 651, 772, 833
BceAI ACGGC 2 cut(s) 149, 924
BcgI CGANNNNNNTGC 2 cut(s) 411, 445
BclI TGATCA 1 cut(s) 37
BcoDI GTCTC 3 cut(s) 137, 350, 751
BfaI CTAG 2 cut(s) 809, 979
BisI GCNGC 2 cut(s) 182, 392
BlsI GCNGC 2 cut(s) 183, 393
BmeT110I CYCGRG 1 cut(s) 45
BmiI GGNNCC 1 cut(s) 707
BmsI GCATC 1 cut(s) 379
BpiI GAAGAC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 775
BsaAI YACGTR 1 cut(s) 546
BsaBI GATNNNNATC 1 cut(s) 504
BsaJI CCNNGG 1 cut(s) 944
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 1 cut(s) 950
Bse1I ACTGG 3 cut(s) 101, 182, 868
Bse3DI GCAATG 1 cut(s) 496
Bse8I GATNNNNATC 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 944
BseGI GGATG 1 cut(s) 662
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 950
BseMI GCAATG 1 cut(s) 496
BseNI ACTGG 3 cut(s) 101, 182, 868
BsePI GCGCGC 1 cut(s) 301
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 1 cut(s) 193
Bsh1236I CGCG 2 cut(s) 303, 391
BshNI GGYRCC 1 cut(s) 705
BsiHKCI CYCGRG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 1 cut(s) 950
BsmAI GTCTC 3 cut(s) 137, 350, 751
BsmBI CGTCTC 2 cut(s) 137, 751
BsmFI GGGAC 1 cut(s) 306
BsoBI CYCGRG 1 cut(s) 45
Bsp1407I TGTACA 1 cut(s) 295
Bsp143I GATC 6 cut(s) 37, 52, 94, 116, 505, 749
BspACI CCGC 1 cut(s) 391
BspFNI CGCG 2 cut(s) 303, 391
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 1 cut(s) 707
BspPI GGATC 2 cut(s) 500, 744
BspT107I GGYRCC 1 cut(s) 705
BsrDI GCAATG 1 cut(s) 496
BsrGI TGTACA 1 cut(s) 295
BsrI ACTGG 3 cut(s) 101, 182, 868
BssECI CCNNGG 1 cut(s) 944
BssHII GCGCGC 1 cut(s) 301
BssMI GATC 6 cut(s) 37, 52, 94, 116, 505, 749
BssNAI GTATAC 1 cut(s) 169
BssSI CACGAG 1 cut(s) 31
Bst1107I GTATAC 1 cut(s) 169
Bst2BI CACGAG 1 cut(s) 31
Bst4CI ACNGT 3 cut(s) 248, 291, 361
Bst6I CTCTTC 1 cut(s) 740
BstAUI TGTACA 1 cut(s) 295
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 3 cut(s) 186, 303, 424
BstDSI CCRYGG 1 cut(s) 944
BstF5I GGATG 1 cut(s) 662
BstFNI CGCG 2 cut(s) 303, 391
BstHHI GCGC 2 cut(s) 303, 305
BstKTI GATC 6 cut(s) 40, 55, 97, 119, 508, 752
BstMAI GTCTC 3 cut(s) 137, 350, 751
BstMBI GATC 6 cut(s) 37, 52, 94, 116, 505, 749
BstNSI RCATGY 1 cut(s) 354
BstUI CGCG 2 cut(s) 303, 391
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 749
BstYI RGATCY 1 cut(s) 749
BstZ17I GTATAC 1 cut(s) 169
BtgI CCRYGG 1 cut(s) 944
BtsCI GGATG 1 cut(s) 662
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 3 cut(s) 186, 303, 424
CciI TCATGA 1 cut(s) 780
CfoI GCGC 2 cut(s) 303, 305
CseI GACGC 1 cut(s) 119
Csp6I GTAC 3 cut(s) 296, 547, 706
CspCI CAANNNNNGTGG 2 cut(s) 102, 137
CviAII CATG 5 cut(s) 351, 469, 578, 653, 781
CviJI RGCY 6 cut(s) 188, 426, 808, 939, 949, 964
CviKI_1 RGCY 6 cut(s) 188, 426, 808, 939, 949, 964
CviQI GTAC 3 cut(s) 296, 547, 706
DpnI GATC 6 cut(s) 39, 54, 96, 118, 507, 751
DpnII GATC 6 cut(s) 37, 52, 94, 116, 505, 749
DrdI GACNNNNNNGTC 1 cut(s) 768
DseDI GACNNNNNNGTC 1 cut(s) 768
Eam1104I CTCTTC 1 cut(s) 740
EarI CTCTTC 1 cut(s) 740
Eco57I CTGAAG 1 cut(s) 194
Eco88I CYCGRG 1 cut(s) 45
EcoRI GAATTC 1 cut(s) 686
Esp3I CGTCTC 2 cut(s) 137, 751
FaeI CATG 5 cut(s) 354, 472, 581, 656, 784
FalI AAGNNNNNCTT 2 cut(s) 737, 769
FaqI GGGAC 1 cut(s) 306
FatI CATG 5 cut(s) 350, 468, 577, 652, 780
FbaI TGATCA 1 cut(s) 37
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 2 cut(s) 182, 392
FokI GGATG 1 cut(s) 669
Fsp4HI GCNGC 2 cut(s) 182, 392
FspBI CTAG 2 cut(s) 809, 979
GlaI GCGC 2 cut(s) 302, 304
GluI GCNGC 2 cut(s) 182, 392
HgaI GACGC 1 cut(s) 119
HhaI GCGC 2 cut(s) 303, 305
Hin1II CATG 5 cut(s) 354, 472, 581, 656, 784
Hin6I GCGC 2 cut(s) 301, 303
HinP1I GCGC 2 cut(s) 301, 303
HincII GTYRAC 2 cut(s) 106, 244
HindII GTYRAC 2 cut(s) 106, 244
HinfI GANTC 2 cut(s) 500, 954
HphI GGTGA 1 cut(s) 854
Hpy166II GTNNAC 4 cut(s) 106, 169, 244, 650
Hpy188I TCNGA 6 cut(s) 94, 196, 346, 505, 799, 959
Hpy8I GTNNAC 4 cut(s) 106, 169, 244, 650
Hpy99I CGWCG 4 cut(s) 200, 203, 219, 594
HpyAV CCTTC 2 cut(s) 218, 797
HpyCH4III ACNGT 3 cut(s) 248, 291, 361
HpyCH4IV ACGT 1 cut(s) 545
HpyCH4V TGCA 4 cut(s) 370, 489, 577, 934
HpySE526I ACGT 1 cut(s) 545
Hsp92II CATG 5 cut(s) 354, 472, 581, 656, 784
HspAI GCGC 2 cut(s) 301, 303
KpnI GGTACC 1 cut(s) 709
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 6 cut(s) 37, 52, 94, 116, 505, 749
LmnI GCTCC 1 cut(s) 832
LpnPI CCDG 9 cut(s) 114, 122, 156, 163, 408, 418, 434, 786, 881
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 1 cut(s) 379
MaeI CTAG 2 cut(s) 809, 979
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 1 cut(s) 879
MalI GATC 6 cut(s) 39, 54, 96, 118, 507, 751
MboI GATC 6 cut(s) 37, 52, 94, 116, 505, 749
MboII GAAGA 4 cut(s) 89, 143, 327, 757
MflI RGATCY 1 cut(s) 749
MluCI AATT 6 cut(s) 10, 365, 477, 511, 686, 848
MlyI GAGTC 1 cut(s) 948
MmeI TCCRAC 5 cut(s) 89, 219, 464, 548, 623
MnlI CCTC 7 cut(s) 387, 403, 409, 511, 597, 630, 722
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 5 cut(s) 233, 711, 792, 828, 903
MvnI CGCG 2 cut(s) 303, 391
NdeII GATC 6 cut(s) 37, 52, 94, 116, 505, 749
NlaIII CATG 5 cut(s) 354, 472, 581, 656, 784
NlaIV GGNNCC 1 cut(s) 707
NmuCI GTSAC 1 cut(s) 879
NspI RCATGY 1 cut(s) 354
PaeR7I CTCGAG 1 cut(s) 45
PagI TCATGA 1 cut(s) 780
PauI GCGCGC 1 cut(s) 301
PcsI WCGNNNNNNNCGW 2 cut(s) 52, 766
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 1 cut(s) 500
PkrI GCNGC 2 cut(s) 183, 393
PleI GAGTC 1 cut(s) 948
PpsI GAGTC 1 cut(s) 948
Ppu21I YACGTR 1 cut(s) 546
PsiI TTATAA 1 cut(s) 539
PspN4I GGNNCC 1 cut(s) 707
PspXI VCTCGAGB 1 cut(s) 45
PsuI RGATCY 1 cut(s) 749
PteI GCGCGC 1 cut(s) 301
RsaI GTAC 3 cut(s) 297, 548, 707
RsaNI GTAC 3 cut(s) 296, 547, 706
SaqAI TTAA 5 cut(s) 233, 711, 792, 828, 903
SatI GCNGC 2 cut(s) 182, 392
Sau3AI GATC 6 cut(s) 37, 52, 94, 116, 505, 749
SchI GAGTC 1 cut(s) 948
SfaNI GCATC 1 cut(s) 379
Sfr274I CTCGAG 1 cut(s) 45
SlaI CTCGAG 1 cut(s) 45
SmlI CTYRAG 2 cut(s) 45, 754
SmoI CTYRAG 2 cut(s) 45, 754
Sse9I AATT 6 cut(s) 10, 365, 477, 511, 686, 848
SsiI CCGC 1 cut(s) 391
SspMI CTAG 2 cut(s) 809, 979
TaaI ACNGT 3 cut(s) 248, 291, 361
TaiI ACGT 1 cut(s) 548
TaqI TCGA 2 cut(s) 46, 431
TasI AATT 6 cut(s) 10, 365, 477, 511, 686, 848
TatI WGTACW 1 cut(s) 295
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 1 cut(s) 500
Tru1I TTAA 5 cut(s) 233, 711, 792, 828, 903
Tru9I TTAA 5 cut(s) 233, 711, 792, 828, 903
TscAI CASTG 1 cut(s) 126
TseFI GTSAC 1 cut(s) 879
TseI GCWGC 1 cut(s) 181
Tsp45I GTSAC 1 cut(s) 879
TspDTI ATGAA 9 cut(s) 20, 470, 594, 610, 699, 758, 797, 816, 861
TspGWI ACGGA 1 cut(s) 933
TspRI CASTG 1 cut(s) 126
XapI RAATTY 4 cut(s) 10, 477, 686, 848
XceI RCATGY 1 cut(s) 354
XhoI CTCGAG 1 cut(s) 45
XmiI GTMKAC 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 2 cut(s) 809, 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.