RLG00000023960

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000673
Physical Location & Seq
Forward (+)
30472253 .. 30473378
1126 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023960

Sequence Viewer

Length: 1086 bp
ATGGCATCATTGTCTGATCTTTCAAACGATATCTTAATCACAATCGGCCTACTTCTTTCTTATTGTGATCTTGTTCGCCTTGCTACACTATGCAAGACTTTCCACCGATTAGCTGTTTATGTGGTTCAACTTCAACGTCGTCGAGATATACCATGGCTGATGAACATTGATCGCTATACGCTTCGCAGTAATATCTTCAAATCTGAGCTTTTTGATTTCCAGTTCAACCAGAGTTTCTCTCTTTCAAAAATTATAGGGAGCCAAGTGCGAGAACTACTCTATATTGGTTCTGTTGTGGCTTCAAAATATGGATGGTTGCTTATAACAGAATCTCGATTTCCGACGGATCCTAAGTACTGTTTTTGTAGTCCTCTTACAAATGAGATCATCGAATTGCCTCCGATAGGGTCGGACGACTATTTCATAGATGCAACGTTCTCCAGCCCTCCAACTTCCTCCGATTGTCTTGTATTCCTGATATGTAAAAGAAGTTTAACTAAAGCAAAAAGAAATATCTGCAGCATAAGCACATGCAATCTCAGAGAAAGAAGATGGACAACTTATACCTCTAATGTTCCTCCGGAAGCCAAAATTTGGGAAGTAGCGTATGTGAATGGAAGTTTTTATTGTGCCTTCACTGACGGTTTAACCGTGCTATTAGGTACCTTCAATGTTGCACTTCAGGACTGGACTATAGTGGACTATTCATCCTCCATAGAGATCAAACGGTATGATTGTCTCTATCTGGTTGATTCTGATGGAGAGCTCTTACTAATGGTTCTTCGTAAGCGTTTTAGGGGTGTTTATAGTGTTTGTCATCAGTTTAATCGATTACGAAAAGATTGGACTCGAATTGAGAGCTTGGGAAATCAAGTGTTGTTTTTCTCAGACAAGGGATCCACTGTAGTTCCAGCAGTTGGGGTCTTTAGTGATTTTGTTAATATGATAGTTTACTTTGGCTCGTCCGACCTCAGGTCTTCTACTTACAAAACTGAAGGCGATTGGAAATATCACCAAACATGGAACACCATCACGTTGCCAATCTGGATTGAACCCCTATATATTTTAGAAAGAAGTCCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

362

Amino Acids

41.66

Weight (kDa)

7.93

Isoelectric Point (pI)

56.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 95 - 318 5.5e-25 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 323
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 662
AccB7I CCANNNNNTGG 2 cut(s) 594, 917
AccIII TCCGGA 1 cut(s) 580
AclI AACGTT 1 cut(s) 434
AclWI GGATC 4 cut(s) 341, 354, 891, 904
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 2 cut(s) 665, 1014
AfaI GTAC 2 cut(s) 356, 664
AfiI CCNNNNNNNGG 4 cut(s) 404, 594, 917, 972
AgsI TTSAA 9 cut(s) 24, 128, 134, 199, 226, 246, 303, 670, 1052
AhdI GACNNNNNGTC 1 cut(s) 973
AluBI AGCT 4 cut(s) 113, 208, 766, 861
AluI AGCT 4 cut(s) 113, 208, 766, 861
Alw21I GWGCWC 1 cut(s) 768
Alw26I GTCTC 1 cut(s) 743
AlwI GGATC 4 cut(s) 341, 354, 891, 904
Aor13HI TCCGGA 1 cut(s) 580
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 519
ApoI RAATTY 1 cut(s) 591
Asp718I GGTACC 1 cut(s) 662
AsuHPI GGTGA 1 cut(s) 1004
AxyI CCTNAGG 1 cut(s) 971
BamHI GGATCC 2 cut(s) 346, 896
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 768
BbsI GAAGAC 1 cut(s) 969
Bbv12I GWGCWC 1 cut(s) 768
BbvI GCAGC 1 cut(s) 531
BccI CCATC 4 cut(s) 306, 546, 752, 1037
BcoDI GTCTC 1 cut(s) 743
BfmI CTRYAG 3 cut(s) 517, 693, 903
BisI GCNGC 1 cut(s) 520
BlsI GCNGC 1 cut(s) 521
BmcAI AGTACT 1 cut(s) 356
BmeRI GACNNNNNGTC 1 cut(s) 973
BmiI GGNNCC 4 cut(s) 260, 348, 664, 898
BmsI GCATC 2 cut(s) 14, 418
BpiI GAAGAC 1 cut(s) 969
BplI GAGNNNNNCTC 4 cut(s) 223, 255, 261, 293
BpmI CTGGAG 1 cut(s) 424
Bsa29I ATCGAT 1 cut(s) 829
BsaJI CCNNGG 1 cut(s) 152
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 4 cut(s) 404, 594, 917, 972
Bse1I ACTGG 2 cut(s) 220, 692
Bse21I CCTNAGG 1 cut(s) 971
BseAI TCCGGA 1 cut(s) 580
BseCI ATCGAT 1 cut(s) 829
BseDI CCNNGG 1 cut(s) 152
BseGI GGATG 2 cut(s) 317, 707
BseLI CCNNNNNNNGG 4 cut(s) 404, 594, 917, 972
BseMII CTCAG 4 cut(s) 195, 553, 900, 985
BseNI ACTGG 2 cut(s) 220, 692
BseXI GCAGC 1 cut(s) 531
BshFI GGCC 1 cut(s) 48
BshNI GGYRCC 1 cut(s) 662
BshVI ATCGAT 1 cut(s) 829
BsiHKAI GWGCWC 1 cut(s) 768
BsiSI CCGG 1 cut(s) 581
BslI CCNNNNNNNGG 4 cut(s) 404, 594, 917, 972
BsmAI GTCTC 1 cut(s) 743
BsnI GGCC 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 768
Bsp13I TCCGGA 1 cut(s) 580
Bsp143I GATC 7 cut(s) 16, 67, 169, 346, 384, 720, 896
Bsp19I CCATGG 1 cut(s) 152
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 4 cut(s) 196, 552, 899, 984
BspDI ATCGAT 1 cut(s) 829
BspEI TCCGGA 1 cut(s) 580
BspLI GGNNCC 4 cut(s) 260, 348, 664, 898
BspMAI CTGCAG 1 cut(s) 521
BspPI GGATC 4 cut(s) 341, 354, 891, 904
BspT107I GGYRCC 1 cut(s) 662
BsrI ACTGG 2 cut(s) 220, 692
BssECI CCNNGG 1 cut(s) 152
BssMI GATC 7 cut(s) 16, 67, 169, 346, 384, 720, 896
BssT1I CCWWGG 1 cut(s) 152
Bst4CI ACNGT 5 cut(s) 359, 644, 652, 729, 904
BstDEI CTNAG 5 cut(s) 204, 351, 539, 886, 971
BstDSI CCRYGG 1 cut(s) 152
BstENI CCTNNNNNAGG 1 cut(s) 402
BstF5I GGATG 2 cut(s) 317, 707
BstKTI GATC 7 cut(s) 19, 70, 172, 349, 387, 723, 899
BstMAI GTCTC 1 cut(s) 743
BstMBI GATC 7 cut(s) 16, 67, 169, 346, 384, 720, 896
BstMWI GCNNNNNNNGC 1 cut(s) 525
BstNSI RCATGY 1 cut(s) 534
BstSFI CTRYAG 3 cut(s) 517, 693, 903
BstV1I GCAGC 1 cut(s) 531
BstV2I GAAGAC 1 cut(s) 969
BstX2I RGATCY 2 cut(s) 346, 896
BstYI RGATCY 2 cut(s) 346, 896
Bsu15I ATCGAT 1 cut(s) 829
Bsu36I CCTNAGG 1 cut(s) 971
BsuRI GGCC 1 cut(s) 48
BsuTUI ATCGAT 1 cut(s) 829
BtgI CCRYGG 1 cut(s) 152
BtsCI GGATG 2 cut(s) 317, 707
BtsIMutI CAGTG 2 cut(s) 636, 900
ClaI ATCGAT 1 cut(s) 829
Csp6I GTAC 2 cut(s) 355, 663
CviAII CATG 3 cut(s) 153, 531, 1020
CviQI GTAC 2 cut(s) 355, 663
DdeI CTNAG 5 cut(s) 204, 351, 539, 886, 971
DpnI GATC 7 cut(s) 18, 69, 171, 348, 386, 722, 898
DpnII GATC 7 cut(s) 16, 67, 169, 346, 384, 720, 896
DriI GACNNNNNGTC 1 cut(s) 973
Eam1105I GACNNNNNGTC 1 cut(s) 973
Ecl136II GAGCTC 1 cut(s) 766
Eco130I CCWWGG 1 cut(s) 152
Eco24I GRGCYC 1 cut(s) 768
Eco32I GATATC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 766
Eco57I CTGAAG 2 cut(s) 665, 1014
Eco81I CCTNAGG 1 cut(s) 971
EcoICRI GAGCTC 1 cut(s) 766
EcoNI CCTNNNNNAGG 1 cut(s) 402
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 152
EcoT38I GRGCYC 1 cut(s) 768
ErhI CCWWGG 1 cut(s) 152
FaeI CATG 3 cut(s) 156, 534, 1023
FalI AAGNNNNNCTT 1 cut(s) 1063
FatI CATG 3 cut(s) 152, 530, 1019
Fnu4HI GCNGC 1 cut(s) 520
FokI GGATG 2 cut(s) 324, 694
FriOI GRGCYC 1 cut(s) 768
Fsp4HI GCNGC 1 cut(s) 520
GluI GCNGC 1 cut(s) 520
GsuI CTGGAG 1 cut(s) 424
HaeIII GGCC 1 cut(s) 48
HapII CCGG 1 cut(s) 581
Hin1II CATG 3 cut(s) 156, 534, 1023
HinfI GANTC 3 cut(s) 329, 752, 847
HpaII CCGG 1 cut(s) 581
HphI GGTGA 1 cut(s) 1004
Hpy166II GTNNAC 2 cut(s) 700, 952
Hpy188III TCNNGA 6 cut(s) 143, 333, 475, 581, 683, 1045
Hpy8I GTNNAC 2 cut(s) 700, 952
Hpy99I CGWCG 3 cut(s) 141, 144, 346
HpyAV CCTTC 3 cut(s) 643, 676, 989
HpyCH4III ACNGT 5 cut(s) 359, 644, 652, 729, 904
HpyCH4IV ACGT 3 cut(s) 136, 434, 1034
HpyCH4V TGCA 5 cut(s) 93, 431, 519, 534, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 525
HpyF3I CTNAG 5 cut(s) 204, 351, 539, 886, 971
HpySE526I ACGT 3 cut(s) 136, 434, 1034
Hsp92II CATG 3 cut(s) 156, 534, 1023
Kpn2I TCCGGA 1 cut(s) 580
KpnI GGTACC 1 cut(s) 666
Kzo9I GATC 7 cut(s) 16, 67, 169, 346, 384, 720, 896
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 1 cut(s) 531
LweI GCATC 2 cut(s) 14, 418
MaeII ACGT 3 cut(s) 136, 434, 1034
MalI GATC 7 cut(s) 18, 69, 171, 348, 386, 722, 898
MboI GATC 7 cut(s) 16, 67, 169, 346, 384, 720, 896
MboII GAAGA 4 cut(s) 187, 561, 773, 969
MflI RGATCY 2 cut(s) 346, 896
MhlI GDGCHC 1 cut(s) 768
MluCI AATT 4 cut(s) 249, 392, 591, 852
MlyI GAGTC 1 cut(s) 841
MmeI TCCRAC 4 cut(s) 365, 390, 473, 990
MnlI CCTC 8 cut(s) 381, 408, 456, 466, 577, 588, 721, 980
MroI TCCGGA 1 cut(s) 580
MseI TTAA 6 cut(s) 35, 494, 647, 825, 939, 1084
MspI CCGG 1 cut(s) 581
MwoI GCNNNNNNNGC 1 cut(s) 525
NcoI CCATGG 1 cut(s) 152
NdeII GATC 7 cut(s) 16, 67, 169, 346, 384, 720, 896
NlaIII CATG 3 cut(s) 156, 534, 1023
NlaIV GGNNCC 4 cut(s) 260, 348, 664, 898
NspI RCATGY 1 cut(s) 534
PcsI WCGNNNNNNNCGW 1 cut(s) 648
PfeI GAWTC 2 cut(s) 329, 752
PflMI CCANNNNNTGG 2 cut(s) 594, 917
PkrI GCNGC 1 cut(s) 521
PleI GAGTC 1 cut(s) 841
PpsI GAGTC 1 cut(s) 841
PsiI TTATAA 1 cut(s) 323
Psp124BI GAGCTC 1 cut(s) 768
Psp1406I AACGTT 1 cut(s) 434
PspN4I GGNNCC 4 cut(s) 260, 348, 664, 898
PstI CTGCAG 1 cut(s) 521
PsuI RGATCY 2 cut(s) 346, 896
RsaI GTAC 2 cut(s) 356, 664
RsaNI GTAC 2 cut(s) 355, 663
SacI GAGCTC 1 cut(s) 768
SaqAI TTAA 6 cut(s) 35, 494, 647, 825, 939, 1084
SatI GCNGC 1 cut(s) 520
Sau3AI GATC 7 cut(s) 16, 67, 169, 346, 384, 720, 896
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 1 cut(s) 841
SduI GDGCHC 1 cut(s) 768
SfaNI GCATC 2 cut(s) 14, 418
SfcI CTRYAG 3 cut(s) 517, 693, 903
Sse9I AATT 4 cut(s) 249, 392, 591, 852
SstI GAGCTC 1 cut(s) 768
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 5 cut(s) 359, 644, 652, 729, 904
TaiI ACGT 3 cut(s) 139, 437, 1037
TaqI TCGA 5 cut(s) 142, 334, 390, 829, 850
TasI AATT 4 cut(s) 249, 392, 591, 852
TatI WGTACW 1 cut(s) 354
TfiI GAWTC 2 cut(s) 329, 752
Tru1I TTAA 6 cut(s) 35, 494, 647, 825, 939, 1084
Tru9I TTAA 6 cut(s) 35, 494, 647, 825, 939, 1084
TscAI CASTG 2 cut(s) 643, 907
TseI GCWGC 1 cut(s) 519
TspDTI ATGAA 3 cut(s) 176, 412, 696
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 2 cut(s) 643, 907
Van91I CCANNNNNTGG 2 cut(s) 594, 917
XagI CCTNNNNNAGG 1 cut(s) 402
XapI RAATTY 1 cut(s) 591
XceI RCATGY 1 cut(s) 534
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.