Rh3BG060900

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000673
Physical Location & Seq
Reverse (-)
4125858 .. 4127239
1382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG060900.1

Sequence Viewer

Length: 1200 bp
ATGTCAAACACTAATGATCGTTCGTGGTCCGATACGCCAATTGAAATCTTAACCTCAGTTGCTGAACGTCTCTCACCTACACCAATCACTGCTGATACCAATCTTTTTCACTTTGGTTCCGTCTGCAATACTTGGCGACAAGCTAGCTTTCGTGCAGGAGTTCGACGTCGTTCAAGTTTGCCATGGCTAATTAACGGGGAATACGAGTTGATTTGCAGAAACTTACCAGCATTGAAGTGTCAGTTCTCCGATTTCTGGTCCAAACAAATTTTATACTCCTATGCAGAAGTCTTACCGAAAAAATTGTGGAGAACCCCTACTGCACATGCTTCAAAAAATGGCTGGTTGCTTTTGTCGACGGATGAGTGTATTAATCCTATTTTAGCTCGTACCTCATTCTTTTTTTTTAATCCTTTTTGTAAGAAGATGGCGATCATCGAGTTACCTGAAAATTTAAATTTTACAGTCAATCAGGAAATAGTAGCATCAACGTTCACAACAAATCCAACTTGTCCCAGTGATTGTCATATCTTTGTAGCGTGTGGTAGATCATCTAACCCACGAAAAATTTGCAGCATATATACTTGTAATCTGAGGGACGGCGAATGGAAAGAGCAGGATGTACACTGTGACATGGAACTATTTGATGTGGCATATATGGACGGAGCTTTCTATTGCGTTTTTTCAGACGGGATTTCGATAGTATTGCATGTCTTCACATTTAGAAGACGGAGCTTTCTGTCGCGTCTTAAGAAAGCCATTGGACAAGCTGATGATGTTGAACAAAGTGAGCCATTCGTTATCTCCTCCTCCTCCTCTTCCTTAAGAAAGAAGGAGAATGATGATCTCTACTTAGTTGAGGCTGATGGGGATCTCTTGTTAGCAATTTGTTCTCTTGATGACACTAGCAGTTATTGTCACATATTCCGGTTGGATCCGTCACGTAAAAAATGGAATCGAATTAGAAACTTGGGAAATCGAGTAATGATTTTGTCTAGCGATGGTGTTGGATCGATTGTAGTTCCTGCGGTGCGAGATGCTAAAGAATTTGCAAACACGATCTTTGTTTATCGAGACTCAAAGCATGAGCATTATATCTGCAAAAGTACTGAAGCCACTTGGATATTTGACAAAGTGCATCATTTAAATCCTCCTGAAGCAGGCAGTGCTCTCATTGAACCCCCTGACTTATGGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

399

Amino Acids

45.53

Weight (kDa)

6.42

Isoelectric Point (pI)

52.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 99 - 359 4.8e-23 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 169
AccI GTMKAC 1 cut(s) 356
AccII CGCG 1 cut(s) 745
AciI CCGC 1 cut(s) 1028
AclI AACGTT 1 cut(s) 491
AclWI GGATC 4 cut(s) 879, 929, 942, 1018
AcsI RAATTY 5 cut(s) 267, 451, 457, 567, 1046
AcuI CTGAAG 2 cut(s) 1131, 1176
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 3 cut(s) 391, 624, 1108
AfiI CCNNNNNNNGG 2 cut(s) 255, 1160
AflII CTTAAG 2 cut(s) 749, 823
AgsI TTSAA 6 cut(s) 44, 174, 235, 333, 782, 1178
AluBI AGCT 6 cut(s) 143, 147, 386, 668, 735, 770
AluI AGCT 6 cut(s) 143, 147, 386, 668, 735, 770
Alw21I GWGCWC 1 cut(s) 1171
Alw26I GTCTC 2 cut(s) 74, 1068
AlwI GGATC 4 cut(s) 879, 929, 942, 1018
AlwNI CAGNNNCTG 1 cut(s) 62
ApeKI GCWGC 1 cut(s) 573
ApoI RAATTY 5 cut(s) 267, 451, 457, 567, 1046
AseI ATTAAT 1 cut(s) 372
AspS9I GGNCC 2 cut(s) 27, 258
AsuHPI GGTGA 1 cut(s) 66
AsuNHI GCTAGC 1 cut(s) 143
AvaII GGWCC 2 cut(s) 27, 258
BamHI GGATCC 1 cut(s) 934
BbsI GAAGAC 2 cut(s) 706, 733
Bbv12I GWGCWC 1 cut(s) 1171
BbvI GCAGC 1 cut(s) 585
BccI CCATC 3 cut(s) 421, 860, 995
BceAI ACGGC 1 cut(s) 616
BcgI CGANNNNNNTGC 4 cut(s) 552, 586, 688, 722
BcoDI GTCTC 2 cut(s) 74, 1068
BfaI CTAG 3 cut(s) 144, 906, 996
BfrI CTTAAG 2 cut(s) 749, 823
BisI GCNGC 1 cut(s) 574
BlsI GCNGC 1 cut(s) 575
BmcAI AGTACT 1 cut(s) 1108
Bme18I GGWCC 2 cut(s) 27, 258
BmgT120I GGNCC 2 cut(s) 27, 258
BmiI GGNNCC 2 cut(s) 118, 936
BmrI ACTGGG 1 cut(s) 510
BmsI GCATC 3 cut(s) 494, 1027, 1147
BmtI GCTAGC 1 cut(s) 147
BmuI ACTGGG 1 cut(s) 510
BpiI GAAGAC 2 cut(s) 706, 733
Bsa29I ATCGAT 1 cut(s) 1013
BsaAI YACGTR 1 cut(s) 944
BsaBI GATNNNNATC 3 cut(s) 99, 431, 870
BsaHI GRCGYC 1 cut(s) 166
BsaJI CCNNGG 1 cut(s) 182
BsaWI WCCGGW 1 cut(s) 927
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 1160
Bse1I ACTGG 1 cut(s) 516
Bse8I GATNNNNATC 3 cut(s) 99, 431, 870
BseCI ATCGAT 1 cut(s) 1013
BseDI CCNNGG 1 cut(s) 182
BseGI GGATG 2 cut(s) 367, 625
BseJI GATNNNNATC 3 cut(s) 99, 431, 870
BseLI CCNNNNNNNGG 2 cut(s) 255, 1160
BseMII CTCAG 2 cut(s) 69, 584
BseNI ACTGG 1 cut(s) 516
BseRI GAGGAG 4 cut(s) 796, 799, 802, 805
BseXI GCAGC 1 cut(s) 585
BsgI GTGCAG 2 cut(s) 174, 306
Bsh1236I CGCG 1 cut(s) 745
BshVI ATCGAT 1 cut(s) 1013
BsiHKAI GWGCWC 1 cut(s) 1171
BsiSI CCGG 1 cut(s) 928
BslFI GGGAC 2 cut(s) 498, 611
BslI CCNNNNNNNGG 2 cut(s) 255, 1160
BsmAI GTCTC 2 cut(s) 74, 1068
BsmBI CGTCTC 1 cut(s) 74
BsmFI GGGAC 2 cut(s) 498, 611
Bsp1286I GDGCHC 1 cut(s) 1171
Bsp1407I TGTACA 1 cut(s) 622
Bsp143I GATC 8 cut(s) 16, 432, 548, 844, 871, 934, 1010, 1059
Bsp19I CCATGG 1 cut(s) 182
BspACI CCGC 1 cut(s) 1028
BspCNI CTCAG 2 cut(s) 68, 585
BspDI ATCGAT 1 cut(s) 1013
BspFNI CGCG 1 cut(s) 745
BspLI GGNNCC 2 cut(s) 118, 936
BspOI GCTAGC 1 cut(s) 147
BspPI GGATC 4 cut(s) 879, 929, 942, 1018
BspTI CTTAAG 2 cut(s) 749, 823
BsrGI TGTACA 1 cut(s) 622
BsrI ACTGG 1 cut(s) 516
BssECI CCNNGG 1 cut(s) 182
BssMI GATC 8 cut(s) 16, 432, 548, 844, 871, 934, 1010, 1059
BssNI GRCGYC 1 cut(s) 166
BssT1I CCWWGG 1 cut(s) 182
Bst4CI ACNGT 2 cut(s) 466, 629
Bst6I CTCTTC 1 cut(s) 823
BstACI GRCGYC 1 cut(s) 166
BstAFI CTTAAG 2 cut(s) 749, 823
BstAPI GCANNNNNTGC 1 cut(s) 1166
BstAUI TGTACA 1 cut(s) 622
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 2 cut(s) 145, 1162
BstDEI CTNAG 3 cut(s) 55, 593, 853
BstDSI CCRYGG 1 cut(s) 182
BstENI CCTNNNNNAGG 1 cut(s) 1158
BstF5I GGATG 2 cut(s) 367, 625
BstFNI CGCG 1 cut(s) 745
BstKTI GATC 8 cut(s) 19, 435, 551, 847, 874, 937, 1013, 1062
BstMAI GTCTC 2 cut(s) 74, 1068
BstMBI GATC 8 cut(s) 16, 432, 548, 844, 871, 934, 1010, 1059
BstMWI GCNNNNNNNGC 1 cut(s) 1166
BstNSI RCATGY 2 cut(s) 329, 713
BstUI CGCG 1 cut(s) 745
BstV1I GCAGC 1 cut(s) 585
BstV2I GAAGAC 2 cut(s) 706, 733
BstX2I RGATCY 2 cut(s) 871, 934
BstYI RGATCY 2 cut(s) 871, 934
Bsu15I ATCGAT 1 cut(s) 1013
BsuTUI ATCGAT 1 cut(s) 1013
BtgI CCRYGG 1 cut(s) 182
BtgZI GCGATG 1 cut(s) 1014
BtsCI GGATG 2 cut(s) 367, 625
BtsI GCAGTG 2 cut(s) 87, 1171
BtsIMutI CAGTG 4 cut(s) 87, 523, 625, 1171
Cac8I GCNNGC 2 cut(s) 145, 1162
CaiI CAGNNNCTG 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 27, 258
ClaI ATCGAT 1 cut(s) 1013
CseI GACGC 1 cut(s) 734
Csp6I GTAC 3 cut(s) 390, 623, 1107
CviAII CATG 5 cut(s) 183, 326, 634, 710, 1085
CviQI GTAC 3 cut(s) 390, 623, 1107
DdeI CTNAG 3 cut(s) 55, 593, 853
DpnI GATC 8 cut(s) 18, 434, 550, 846, 873, 936, 1012, 1061
DpnII GATC 8 cut(s) 16, 432, 548, 844, 871, 934, 1010, 1059
DraI TTTAAA 2 cut(s) 456, 1146
Eam1104I CTCTTC 1 cut(s) 823
EarI CTCTTC 1 cut(s) 823
Eco130I CCWWGG 1 cut(s) 182
Eco47I GGWCC 2 cut(s) 27, 258
Eco57I CTGAAG 2 cut(s) 1131, 1176
EcoNI CCTNNNNNAGG 1 cut(s) 1158
EcoT14I CCWWGG 1 cut(s) 182
ErhI CCWWGG 1 cut(s) 182
Esp3I CGTCTC 1 cut(s) 74
FaeI CATG 5 cut(s) 186, 329, 637, 713, 1088
FaqI GGGAC 2 cut(s) 498, 611
FatI CATG 5 cut(s) 182, 325, 633, 709, 1084
FblI GTMKAC 1 cut(s) 356
Fnu4HI GCNGC 1 cut(s) 574
FokI GGATG 2 cut(s) 374, 632
Fsp4HI GCNGC 1 cut(s) 574
FspBI CTAG 3 cut(s) 144, 906, 996
GluI GCNGC 1 cut(s) 574
HapII CCGG 1 cut(s) 928
HgaI GACGC 1 cut(s) 734
Hin1I GRCGYC 1 cut(s) 166
Hin1II CATG 5 cut(s) 186, 329, 637, 713, 1088
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 2 cut(s) 955, 1076
HpaII CCGG 1 cut(s) 928
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 3 cut(s) 357, 495, 625
Hpy188I TCNGA 4 cut(s) 31, 250, 594, 688
Hpy188III TCNNGA 4 cut(s) 473, 896, 1073, 1154
Hpy8I GTNNAC 3 cut(s) 357, 495, 625
Hpy99I CGWCG 3 cut(s) 168, 171, 361
HpyAV CCTTC 1 cut(s) 826
HpyCH4III ACNGT 2 cut(s) 466, 629
HpyCH4IV ACGT 4 cut(s) 67, 166, 491, 943
HpyF10VI GCNNNNNNNGC 1 cut(s) 1166
HpyF3I CTNAG 3 cut(s) 55, 593, 853
HpySE526I ACGT 4 cut(s) 67, 166, 491, 943
Hsp92I GRCGYC 1 cut(s) 166
Hsp92II CATG 5 cut(s) 186, 329, 637, 713, 1088
Kzo9I GATC 8 cut(s) 16, 432, 548, 844, 871, 934, 1010, 1059
LmnI GCTCC 2 cut(s) 665, 732
Lsp1109I GCAGC 1 cut(s) 585
LweI GCATC 3 cut(s) 494, 1027, 1147
MaeI CTAG 3 cut(s) 144, 906, 996
MaeII ACGT 4 cut(s) 67, 166, 491, 943
MaeIII GTNAC 4 cut(s) 441, 629, 917, 939
MalI GATC 8 cut(s) 18, 434, 550, 846, 873, 936, 1012, 1061
MboI GATC 8 cut(s) 16, 432, 548, 844, 871, 934, 1010, 1059
MboII GAAGA 4 cut(s) 436, 706, 738, 810
MfeI CAATTG 1 cut(s) 39
MflI RGATCY 2 cut(s) 871, 934
MhlI GDGCHC 1 cut(s) 1171
MlyI GAGTC 1 cut(s) 1070
MmeI TCCRAC 3 cut(s) 530, 912, 988
MnlI CCTC 9 cut(s) 64, 403, 588, 817, 820, 823, 826, 853, 1161
MseI TTAA 8 cut(s) 50, 192, 372, 408, 455, 750, 824, 1145
MslI CAYNNNNRTG 1 cut(s) 235
MspCI CTTAAG 2 cut(s) 749, 823
MspI CCGG 1 cut(s) 928
MunI CAATTG 1 cut(s) 39
MvnI CGCG 1 cut(s) 745
MwoI GCNNNNNNNGC 1 cut(s) 1166
NcoI CCATGG 1 cut(s) 182
NdeII GATC 8 cut(s) 16, 432, 548, 844, 871, 934, 1010, 1059
NheI GCTAGC 1 cut(s) 143
NlaIII CATG 5 cut(s) 186, 329, 637, 713, 1088
NlaIV GGNNCC 2 cut(s) 118, 936
NmuCI GTSAC 3 cut(s) 629, 917, 939
NspI RCATGY 2 cut(s) 329, 713
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 1 cut(s) 955
PkrI GCNGC 1 cut(s) 575
PleI GAGTC 1 cut(s) 1070
PpsI GAGTC 1 cut(s) 1070
Ppu21I YACGTR 1 cut(s) 944
PshBI ATTAAT 1 cut(s) 372
Psp1406I AACGTT 1 cut(s) 491
PspN4I GGNNCC 2 cut(s) 118, 936
PspPI GGNCC 2 cut(s) 27, 258
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 2 cut(s) 871, 934
RsaI GTAC 3 cut(s) 391, 624, 1108
RsaNI GTAC 3 cut(s) 390, 623, 1107
RseI CAYNNNNRTG 1 cut(s) 235
SalI GTCGAC 1 cut(s) 355
SaqAI TTAA 8 cut(s) 50, 192, 372, 408, 455, 750, 824, 1145
SatI GCNGC 1 cut(s) 574
Sau3AI GATC 8 cut(s) 16, 432, 548, 844, 871, 934, 1010, 1059
Sau96I GGNCC 2 cut(s) 27, 258
ScaI AGTACT 1 cut(s) 1108
SchI GAGTC 1 cut(s) 1070
SduI GDGCHC 1 cut(s) 1171
SfaNI GCATC 3 cut(s) 494, 1027, 1147
SinI GGWCC 2 cut(s) 27, 258
SmiI ATTTAAAT 2 cut(s) 456, 1146
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 2 cut(s) 749, 823
SmoI CTYRAG 2 cut(s) 749, 823
SsiI CCGC 1 cut(s) 1028
SspMI CTAG 3 cut(s) 144, 906, 996
StyI CCWWGG 1 cut(s) 182
SwaI ATTTAAAT 2 cut(s) 456, 1146
TaaI ACNGT 2 cut(s) 466, 629
TaiI ACGT 4 cut(s) 70, 169, 494, 946
TaqI TCGA 8 cut(s) 163, 356, 438, 698, 958, 979, 1013, 1072
TatI WGTACW 2 cut(s) 622, 1106
TfiI GAWTC 1 cut(s) 955
Tru1I TTAA 8 cut(s) 50, 192, 372, 408, 455, 750, 824, 1145
Tru9I TTAA 8 cut(s) 50, 192, 372, 408, 455, 750, 824, 1145
TscAI CASTG 4 cut(s) 94, 523, 632, 1171
TseFI GTSAC 3 cut(s) 629, 917, 939
TseI GCWGC 1 cut(s) 573
Tsp45I GTSAC 3 cut(s) 629, 917, 939
TspGWI ACGGA 5 cut(s) 109, 374, 678, 745, 927
TspRI CASTG 4 cut(s) 94, 523, 632, 1171
Vha464I CTTAAG 2 cut(s) 749, 823
VpaK11BI GGWCC 2 cut(s) 27, 258
VspI ATTAAT 1 cut(s) 372
XagI CCTNNNNNAGG 1 cut(s) 1158
XapI RAATTY 5 cut(s) 267, 451, 457, 567, 1046
XceI RCATGY 2 cut(s) 329, 713
XmiI GTMKAC 1 cut(s) 356
XspI CTAG 3 cut(s) 144, 906, 996
ZraI GACGTC 1 cut(s) 167
ZrmI AGTACT 1 cut(s) 1108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.