Rorug04G0209000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000673
Physical Location & Seq
Reverse (-)
36453973 .. 36455944
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0209000.1

Sequence Viewer

Length: 1068 bp
ATGCCAAAGGAATTAGCAAAAGAAACGCAGGTAATGAATCCAAAGCCAAATAACAAGCATCCCCAAGTGGAATCTGGAAAGCACAGGAGCAACACTGAACCACTGAGGAAGAAGGAGAAGGAGAAGAAGAAGAAGGGTCAGCCGAGCATTATGGGTAAAGTAAGACGAAGATGGGAGGACTTAGAAAGGGACTGCTTGGTGAATGTTTTCAGAAGAGTGGGTATGGAGTCACTGCTATTCGATGTGCCTTTCGTGTGCAAGTCATGGTTTACAGCAACCCTTGATCCTTCATGCTGGCAATATCTCATTTTTCCAGATATTCCATCTGATCTTGCTGACCGTTTGGACAATATATTTTCTTTTATTTATCCTAGTGATTCCATTGAGCTTTATCCTGATCACTTTGATCCCTTAATAAGAAGATATATAGGAGCATATCGATTTGGATCAAGCCACGTATCTATTACTGCTTTTGTTGAGTTTGTCCTTCGTCGGAGCAATGGAAATGTCGTGTTTCTGAAGGTACCTCGCCACTGTCCAAAAGCTGCTCTAAAGTCTGTAGCACATGCGTGTCCTGACCTGAAGGCTTTGGCTTTGCCGAGAGACGCGTTGAATTCAGATGTAGTTCCTGAATTGGTTGGCAGCTTGACAAGTTTGAGGTCATTGTTGTTATTGGGAAGCTGCAATTATATTGAGAGATTTTTATTACCAATCAGCAAGTACTGCAAGAAATTTCGTGGTTTATGTTTTTATGAGGCTTATATTACTGAAGAGGCTGCATCTGCGATTGTTAACTCATTGCCGAATATCAAGTACTTGATTCTGAGGAGCACTTTGATTAGACCAGATGGTTTGATCACATTGCTGCGCGGCTGCAAACAGCTTGTGCTGTTGCATCTCAGTGAGTGTCCCTGTTTTCGGGTCAACGATGAGATACTGAATCTGGCATCTCGTATTAGCGACTTCAGATTTTCCAATCATTTTGGAGGTTTTCAAGGTTATTATGTGGACGATGAGCTAGGCTATATTAATTTTGATGAAGGCACCAGTGACGACGACTATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

355

Amino Acids

40.66

Weight (kDa)

8.39

Isoelectric Point (pI)

51.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 19
Acc65I GGTACC 1 cut(s) 523
AccB1I GGYRCC 2 cut(s) 523, 1043
AccII CGCG 2 cut(s) 608, 870
AciI CCGC 1 cut(s) 870
AclWI GGATC 3 cut(s) 278, 401, 454
AcsI RAATTY 2 cut(s) 613, 731
AcuI CTGAAG 4 cut(s) 539, 602, 789, 949
AfaI GTAC 3 cut(s) 525, 722, 815
AfiI CCNNNNNNNGG 1 cut(s) 918
AflIII ACRYGT 1 cut(s) 606
AgsI TTSAA 2 cut(s) 613, 995
AleI CACNNNNGTG 1 cut(s) 568
AluBI AGCT 6 cut(s) 388, 545, 645, 681, 883, 1018
AluI AGCT 6 cut(s) 388, 545, 645, 681, 883, 1018
Alw21I GWGCWC 1 cut(s) 833
Alw26I GTCTC 1 cut(s) 597
AlwI GGATC 3 cut(s) 278, 401, 454
ApeKI GCWGC 6 cut(s) 545, 642, 681, 776, 865, 873
ApoI RAATTY 2 cut(s) 613, 731
AseI ATTAAT 1 cut(s) 1029
Asp700I GAANNNNTTC 1 cut(s) 206
Asp718I GGTACC 1 cut(s) 523
AspLEI GCGC 1 cut(s) 870
AsuHPI GGTGA 1 cut(s) 211
BanI GGYRCC 2 cut(s) 523, 1043
Bbv12I GWGCWC 1 cut(s) 833
BbvI GCAGC 6 cut(s) 532, 654, 668, 763, 852, 860
BccI CCATC 3 cut(s) 165, 331, 842
BclI TGATCA 2 cut(s) 397, 855
BcoDI GTCTC 1 cut(s) 597
BfaI CTAG 2 cut(s) 372, 1019
BfmI CTRYAG 1 cut(s) 558
BfuAI ACCTGC 1 cut(s) 19
BisI GCNGC 7 cut(s) 546, 643, 682, 777, 866, 871, 874
BlsI GCNGC 7 cut(s) 547, 644, 683, 778, 867, 872, 875
BmcAI AGTACT 2 cut(s) 722, 815
BmiI GGNNCC 2 cut(s) 525, 1045
BmsI GCATC 4 cut(s) 67, 788, 904, 956
Bsa29I ATCGAT 1 cut(s) 439
BsaAI YACGTR 1 cut(s) 457
BsaBI GATNNNNATC 1 cut(s) 445
Bsc4I CCNNNNNNNGG 1 cut(s) 918
Bse1I ACTGG 1 cut(s) 1047
Bse3DI GCAATG 3 cut(s) 505, 797, 860
Bse8I GATNNNNATC 1 cut(s) 445
BseCI ATCGAT 1 cut(s) 439
BseGI GGATG 1 cut(s) 58
BseJI GATNNNNATC 1 cut(s) 445
BseLI CCNNNNNNNGG 1 cut(s) 918
BseMI GCAATG 3 cut(s) 505, 797, 860
BseMII CTCAG 3 cut(s) 95, 815, 913
BseNI ACTGG 1 cut(s) 1047
BseRI GAGGAG 1 cut(s) 841
BseXI GCAGC 6 cut(s) 532, 654, 668, 763, 852, 860
Bsh1236I CGCG 2 cut(s) 608, 870
BshNI GGYRCC 2 cut(s) 523, 1043
BshVI ATCGAT 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 833
BslFI GGGAC 2 cut(s) 203, 894
BslI CCNNNNNNNGG 1 cut(s) 918
BsmAI GTCTC 1 cut(s) 597
BsmBI CGTCTC 1 cut(s) 597
BsmFI GGGAC 2 cut(s) 203, 894
Bsp1286I GDGCHC 1 cut(s) 833
Bsp143I GATC 6 cut(s) 283, 328, 397, 406, 446, 855
BspACI CCGC 1 cut(s) 870
BspCNI CTCAG 3 cut(s) 96, 816, 912
BspDI ATCGAT 1 cut(s) 439
BspFNI CGCG 2 cut(s) 608, 870
BspLI GGNNCC 2 cut(s) 525, 1045
BspMI ACCTGC 1 cut(s) 19
BspPI GGATC 3 cut(s) 278, 401, 454
BspT107I GGYRCC 2 cut(s) 523, 1043
BsrDI GCAATG 3 cut(s) 505, 797, 860
BsrI ACTGG 1 cut(s) 1047
BssMI GATC 6 cut(s) 283, 328, 397, 406, 446, 855
Bst4CI ACNGT 2 cut(s) 341, 536
Bst6I CTCTTC 2 cut(s) 208, 765
BstAPI GCANNNNNTGC 1 cut(s) 723
BstBAI YACGTR 1 cut(s) 457
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 4 cut(s) 104, 181, 824, 899
BstF5I GGATG 1 cut(s) 58
BstFNI CGCG 2 cut(s) 608, 870
BstHHI GCGC 1 cut(s) 870
BstKTI GATC 6 cut(s) 286, 331, 400, 409, 449, 858
BstMAI GTCTC 1 cut(s) 597
BstMBI GATC 6 cut(s) 283, 328, 397, 406, 446, 855
BstMWI GCNNNNNNNGC 2 cut(s) 723, 782
BstNSI RCATGY 1 cut(s) 569
BstSFI CTRYAG 1 cut(s) 558
BstUI CGCG 2 cut(s) 608, 870
BstV1I GCAGC 6 cut(s) 532, 654, 668, 763, 852, 860
Bsu15I ATCGAT 1 cut(s) 439
BsuTUI ATCGAT 1 cut(s) 439
BtsCI GGATG 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 230
BtsIMutI CAGTG 6 cut(s) 93, 101, 230, 532, 907, 1054
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 296
CfoI GCGC 1 cut(s) 870
ClaI ATCGAT 1 cut(s) 439
CseI GACGC 1 cut(s) 614
Csp6I GTAC 3 cut(s) 524, 721, 814
CviAII CATG 3 cut(s) 264, 291, 566
CviQI GTAC 3 cut(s) 524, 721, 814
DdeI CTNAG 4 cut(s) 104, 181, 824, 899
DpnI GATC 6 cut(s) 285, 330, 399, 408, 448, 857
DpnII GATC 6 cut(s) 283, 328, 397, 406, 446, 855
Eam1104I CTCTTC 2 cut(s) 208, 765
EarI CTCTTC 2 cut(s) 208, 765
Eco57I CTGAAG 4 cut(s) 539, 602, 789, 949
EcoRI GAATTC 1 cut(s) 613
Esp3I CGTCTC 1 cut(s) 597
FaeI CATG 3 cut(s) 267, 294, 569
FaqI GGGAC 2 cut(s) 203, 894
FatI CATG 3 cut(s) 263, 290, 565
FbaI TGATCA 2 cut(s) 397, 855
Fnu4HI GCNGC 7 cut(s) 546, 643, 682, 777, 866, 871, 874
FokI GGATG 1 cut(s) 45
Fsp4HI GCNGC 7 cut(s) 546, 643, 682, 777, 866, 871, 874
FspBI CTAG 2 cut(s) 372, 1019
GlaI GCGC 1 cut(s) 869
GluI GCNGC 7 cut(s) 546, 643, 682, 777, 866, 871, 874
HgaI GACGC 1 cut(s) 614
HhaI GCGC 1 cut(s) 870
Hin1II CATG 3 cut(s) 267, 294, 569
Hin6I GCGC 1 cut(s) 868
HinP1I GCGC 1 cut(s) 868
HincII GTYRAC 2 cut(s) 793, 925
HindII GTYRAC 2 cut(s) 793, 925
HinfI GANTC 6 cut(s) 37, 71, 227, 377, 820, 940
HpaI GTTAAC 1 cut(s) 793
HphI GGTGA 1 cut(s) 211
Hpy166II GTNNAC 4 cut(s) 270, 793, 925, 1009
Hpy188I TCNGA 7 cut(s) 212, 328, 495, 519, 619, 825, 968
Hpy188III TCNNGA 5 cut(s) 75, 314, 395, 575, 629
Hpy8I GTNNAC 4 cut(s) 270, 793, 925, 1009
Hpy99I CGWCG 2 cut(s) 495, 1058
HpyAV CCTTC 8 cut(s) 106, 112, 127, 297, 497, 514, 577, 1034
HpyCH4III ACNGT 2 cut(s) 341, 536
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 6 cut(s) 258, 684, 726, 779, 876, 895
HpyF10VI GCNNNNNNNGC 2 cut(s) 723, 782
HpyF3I CTNAG 4 cut(s) 104, 181, 824, 899
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 3 cut(s) 267, 294, 569
HspAI GCGC 1 cut(s) 868
KpnI GGTACC 1 cut(s) 527
Ksp22I TGATCA 2 cut(s) 397, 855
KspAI GTTAAC 1 cut(s) 793
Kzo9I GATC 6 cut(s) 283, 328, 397, 406, 446, 855
LmnI GCTCC 4 cut(s) 87, 431, 495, 828
Lsp1109I GCAGC 6 cut(s) 532, 654, 668, 763, 852, 860
LweI GCATC 4 cut(s) 67, 788, 904, 956
MaeI CTAG 2 cut(s) 372, 1019
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 2 cut(s) 228, 1049
MalI GATC 6 cut(s) 285, 330, 399, 408, 448, 857
MboI GATC 6 cut(s) 283, 328, 397, 406, 446, 855
MboII GAAGA 8 cut(s) 121, 136, 139, 142, 180, 225, 432, 782
MhlI GDGCHC 1 cut(s) 833
MluCI AATT 6 cut(s) 11, 613, 632, 685, 731, 1030
MluI ACGCGT 1 cut(s) 606
MlyI GAGTC 1 cut(s) 236
MmeI TCCRAC 1 cut(s) 473
MnlI CCTC 8 cut(s) 99, 169, 537, 651, 748, 766, 819, 980
MroXI GAANNNNTTC 1 cut(s) 206
MseI TTAA 4 cut(s) 413, 792, 1029, 1066
MslI CAYNNNNRTG 2 cut(s) 568, 900
MvnI CGCG 2 cut(s) 608, 870
MwoI GCNNNNNNNGC 2 cut(s) 723, 782
NdeII GATC 6 cut(s) 283, 328, 397, 406, 446, 855
NlaIII CATG 3 cut(s) 267, 294, 569
NlaIV GGNNCC 2 cut(s) 525, 1045
NmeAIII GCCGAG 2 cut(s) 168, 624
NmuCI GTSAC 2 cut(s) 228, 1049
NspI RCATGY 1 cut(s) 569
OliI CACNNNNGTG 1 cut(s) 568
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 5 cut(s) 37, 71, 377, 820, 940
PkrI GCNGC 7 cut(s) 547, 644, 683, 778, 867, 872, 875
PleI GAGTC 1 cut(s) 235
PpsI GAGTC 1 cut(s) 235
Ppu21I YACGTR 1 cut(s) 457
PshBI ATTAAT 1 cut(s) 1029
PspN4I GGNNCC 2 cut(s) 525, 1045
RsaI GTAC 3 cut(s) 525, 722, 815
RsaNI GTAC 3 cut(s) 524, 721, 814
RseI CAYNNNNRTG 2 cut(s) 568, 900
SaqAI TTAA 4 cut(s) 413, 792, 1029, 1066
SatI GCNGC 7 cut(s) 546, 643, 682, 777, 866, 871, 874
Sau3AI GATC 6 cut(s) 283, 328, 397, 406, 446, 855
ScaI AGTACT 2 cut(s) 722, 815
SchI GAGTC 1 cut(s) 236
SduI GDGCHC 1 cut(s) 833
SfaNI GCATC 4 cut(s) 67, 788, 904, 956
SfcI CTRYAG 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 568, 900
Sse9I AATT 6 cut(s) 11, 613, 632, 685, 731, 1030
SsiI CCGC 1 cut(s) 870
SspMI CTAG 2 cut(s) 372, 1019
TaaI ACNGT 2 cut(s) 341, 536
TaiI ACGT 1 cut(s) 459
TaqI TCGA 2 cut(s) 240, 439
TasI AATT 6 cut(s) 11, 613, 632, 685, 731, 1030
TatI WGTACW 2 cut(s) 720, 813
TauI GCSGC 1 cut(s) 873
TfiI GAWTC 5 cut(s) 37, 71, 377, 820, 940
Tru1I TTAA 4 cut(s) 413, 792, 1029, 1066
Tru9I TTAA 4 cut(s) 413, 792, 1029, 1066
TscAI CASTG 6 cut(s) 100, 108, 237, 539, 907, 1054
TseFI GTSAC 2 cut(s) 228, 1049
TseI GCWGC 6 cut(s) 545, 642, 681, 776, 865, 873
Tsp45I GTSAC 2 cut(s) 228, 1049
TspDTI ATGAA 3 cut(s) 50, 279, 1053
TspRI CASTG 6 cut(s) 100, 108, 237, 539, 907, 1054
VspI ATTAAT 1 cut(s) 1029
XapI RAATTY 2 cut(s) 613, 731
XceI RCATGY 1 cut(s) 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 71
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 2 cut(s) 372, 1019
ZrmI AGTACT 2 cut(s) 722, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.