Rroxscaffold_1G00043780

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000673
Physical Location & Seq
Forward (+)
62469662 .. 62470774
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043780.1

Sequence Viewer

Length: 1113 bp
ATGAGAGAATCACACCACCATTGTTGGTCTGATCTTCCGGTTGATCTGTTAAGAGAAGTGACTGAACGTCTCTCATACGGAGATGTTCTTCGCTTTGGCGTCGTATGCAAAAAATGGCAGCTAGCTATTCCCGACGACCGACGTGTAATACTGTTGCTGGGCCGCTGTTACAGGCGTATGCGCAGCCGAGGTGCTATTGAAACCAACTGTGAAGCCTGGTACGTTGATTTGACGTCGCAAGAGATTTCGAGATTCTACATCACAAACGTGGTCGTGGTACAAAAACAACGAAAAGGATGTTCTTCGATTCTGAATACAGTTTATTGTTGTCCGAATTCCGGTTGGTTGCTTTTATTTAGATCCAGCCATACAAGGAGAGAAGATACGACATTCTTTGTTCATAATCCTCTTCGAAAAGAGACTATAGAATTCGCATTGCCTGCCCAATTATTAAAGATGGACTTAGCAAAGGTCGAATTAGCCACCATGACGACCGCTCCAACTTCTCCCGATTGTGTTATTTTTATCCTATGGTTCACCGCTTACAGTGAGGAGCACAGATATTTCACTATAACAACATATAACCAACAAGATAAAAGATGGAGTATCTTTAGAGTTCCCCGTTTTGAAAGATTAATGAGGCCCTACATCCTTCCCTGTTTTGATGGTGAAATCCGGGATGTGGTTTATGCGGATGGAATTTTCTATGTTCTTGTTGTTGTTATCAACTCGTCGGAAATTATATTGGGAACCTTCGATGCAGCACTTCAACAGTGGGTTCTAATGGACAATCCCACCTCCACCCTCAAACTACCATATCGTCCAAAACATCTTTACTTGGTTAAATCTGATGGGGAACTCTTATTGGCCGTTTATCATCGAGAGCAAGGTTGTGGTGAATTTTTGTGCCCGGAGGTTTATCGTTTTGATTATTTGTCACAAAAGAATTGGCCAAGACTTGATACTTTAGGAGGCCGAGTTCTGATTTTAAGTCCCTCATCATCAATTTTGACTCCAGCAGTTGGGGCTATAAGTAAATTTGCAAACATAATATATGTTGAGGGTGTGAAAGGCATCCGCATCCCTACATTGGAGATATACAACATTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

370

Amino Acids

42.89

Weight (kDa)

8.56

Isoelectric Point (pI)

48.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 9 - 47 5.1e-08 F-box domain
F-box-like PF12937 9 - 46 7.4e-06 F-box-like
Beta-prop_KIB1-4 PF03478 106 - 352 7e-18 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 236
Acc16I TGCGCA 1 cut(s) 182
AccB7I CCANNNNNTGG 1 cut(s) 1022
AccBSI CCGCTC 1 cut(s) 497
AciI CCGC 5 cut(s) 163, 495, 540, 692, 1078
AclWI GGATC 1 cut(s) 354
AcoI YGGCCR 2 cut(s) 867, 950
AcsI RAATTY 5 cut(s) 334, 428, 699, 899, 1037
AcyI GRCGYC 2 cut(s) 99, 233
AfaI GTAC 2 cut(s) 221, 279
AfiI CCNNNNNNNGG 4 cut(s) 338, 682, 1022, 1090
AflIII ACRYGT 1 cut(s) 142
AgsI TTSAA 3 cut(s) 200, 629, 770
AhdI GACNNNNNGTC 1 cut(s) 66
AjiI CACGTC 1 cut(s) 143
AjnI CCWGG 1 cut(s) 215
AleI CACNNNNGTG 1 cut(s) 266
AloI GAACNNNNNNTCC 2 cut(s) 963, 995
AluBI AGCT 2 cut(s) 121, 125
AluI AGCT 2 cut(s) 121, 125
Alw21I GWGCWC 1 cut(s) 558
Alw26I GTCTC 2 cut(s) 74, 413
AlwI GGATC 1 cut(s) 354
AoxI GGCC 5 cut(s) 160, 641, 867, 950, 973
ApeKI GCWGC 3 cut(s) 118, 183, 761
ApoI RAATTY 5 cut(s) 334, 428, 699, 899, 1037
AseI ATTAAT 1 cut(s) 635
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 2 cut(s) 160, 642
AsuC2I CCSGG 2 cut(s) 677, 911
AsuHPI GGTGA 3 cut(s) 529, 680, 908
AsuII TTCGAA 1 cut(s) 412
AsuNHI GCTAGC 1 cut(s) 121
BaeGI GKGCMC 1 cut(s) 911
BalI TGGCCA 1 cut(s) 952
Bbv12I GWGCWC 1 cut(s) 558
BbvI GCAGC 3 cut(s) 130, 195, 773
BccI CCATC 5 cut(s) 451, 594, 659, 689, 845
BceAI ACGGC 1 cut(s) 854
BciT130I CCWGG 1 cut(s) 217
BcnI CCSGG 2 cut(s) 677, 911
BcoDI GTCTC 2 cut(s) 74, 413
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 1 cut(s) 423
BisI GCNGC 4 cut(s) 119, 163, 184, 762
BlsI GCNGC 4 cut(s) 120, 164, 185, 763
Bme1390I CCNGG 3 cut(s) 217, 677, 911
BmeRI GACNNNNNGTC 1 cut(s) 66
BmgBI CACGTC 1 cut(s) 143
BmgT120I GGNCC 2 cut(s) 160, 642
BmiI GGNNCC 1 cut(s) 751
BmrFI CCNGG 3 cut(s) 217, 677, 911
BmsI GCATC 3 cut(s) 748, 1083, 1089
BmtI GCTAGC 1 cut(s) 125
BpmI CTGGAG 1 cut(s) 999
Bpu14I TTCGAA 1 cut(s) 412
BpuMI CCSGG 2 cut(s) 677, 911
BsaHI GRCGYC 2 cut(s) 99, 233
BsaJI CCNNGG 1 cut(s) 187
BsaWI WCCGGW 2 cut(s) 37, 338
BsaXI ACNNNNNCTCC 4 cut(s) 481, 511, 963, 993
Bsc4I CCNNNNNNNGG 4 cut(s) 338, 682, 1022, 1090
Bse3DI GCAATG 1 cut(s) 434
BseBI CCWGG 1 cut(s) 217
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 6 cut(s) 302, 648, 685, 700, 1074, 1080
BseLI CCNNNNNNNGG 4 cut(s) 338, 682, 1022, 1090
BseMI GCAATG 1 cut(s) 434
BseRI GAGGAG 1 cut(s) 566
BseSI GKGCMC 1 cut(s) 911
BseXI GCAGC 3 cut(s) 130, 195, 773
BseYI CCCAGC 1 cut(s) 157
Bsh1285I CGRYCG 2 cut(s) 139, 495
BshFI GGCC 5 cut(s) 162, 643, 869, 952, 975
BsiEI CGRYCG 2 cut(s) 139, 495
BsiHKAI GWGCWC 1 cut(s) 558
BsiSI CCGG 4 cut(s) 38, 339, 676, 911
BslFI GGGAC 1 cut(s) 978
BslI CCNNNNNNNGG 4 cut(s) 338, 682, 1022, 1090
BsmAI GTCTC 2 cut(s) 74, 413
BsmBI CGTCTC 1 cut(s) 74
BsmFI GGGAC 1 cut(s) 978
BsnI GGCC 5 cut(s) 162, 643, 869, 952, 975
Bsp119I TTCGAA 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 558, 911
Bsp143I GATC 3 cut(s) 31, 43, 359
BspACI CCGC 5 cut(s) 163, 495, 540, 692, 1078
BspANI GGCC 5 cut(s) 162, 643, 869, 952, 975
BspLI GGNNCC 1 cut(s) 751
BspOI GCTAGC 1 cut(s) 125
BspPI GGATC 1 cut(s) 354
BspT104I TTCGAA 1 cut(s) 412
BsrBI CCGCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 434
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 3 cut(s) 31, 43, 359
BssNI GRCGYC 2 cut(s) 99, 233
Bst2UI CCWGG 1 cut(s) 217
Bst4CI ACNGT 5 cut(s) 153, 209, 319, 548, 774
Bst6I CTCTTC 1 cut(s) 414
BstACI GRCGYC 2 cut(s) 99, 233
BstAPI GCANNNNNTGC 1 cut(s) 440
BstBI TTCGAA 1 cut(s) 412
BstC8I GCNNGC 2 cut(s) 123, 441
BstDEI CTNAG 1 cut(s) 463
BstF5I GGATG 6 cut(s) 302, 648, 685, 700, 1074, 1080
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 3 cut(s) 34, 46, 362
BstMAI GTCTC 2 cut(s) 74, 413
BstMBI GATC 3 cut(s) 31, 43, 359
BstMCI CGRYCG 2 cut(s) 139, 495
BstMWI GCNNNNNNNGC 3 cut(s) 105, 440, 1025
BstNI CCWGG 1 cut(s) 217
BstSCI CCNGG 3 cut(s) 215, 675, 909
BstSFI CTRYAG 1 cut(s) 423
BstSLI GKGCMC 1 cut(s) 911
BstV1I GCAGC 3 cut(s) 130, 195, 773
BstX2I RGATCY 1 cut(s) 359
BstYI RGATCY 1 cut(s) 359
BsuRI GGCC 5 cut(s) 162, 643, 869, 952, 975
BtrI CACGTC 1 cut(s) 143
BtsCI GGATG 6 cut(s) 302, 648, 685, 700, 1074, 1080
BtsIMutI CAGTG 2 cut(s) 553, 779
Cac8I GCNNGC 2 cut(s) 123, 441
CfoI GCGC 1 cut(s) 183
Cfr13I GGNCC 2 cut(s) 160, 642
CseI GACGC 1 cut(s) 88
Csp6I GTAC 2 cut(s) 220, 278
CspCI CAANNNNNGTGG 2 cut(s) 5, 40
CviAII CATG 1 cut(s) 487
CviQI GTAC 2 cut(s) 220, 278
DdeI CTNAG 1 cut(s) 463
DpnI GATC 3 cut(s) 33, 45, 361
DpnII GATC 3 cut(s) 31, 43, 359
DriI GACNNNNNGTC 1 cut(s) 66
EaeI YGGCCR 2 cut(s) 867, 950
Eam1104I CTCTTC 1 cut(s) 414
Eam1105I GACNNNNNGTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 414
EcoO109I RGGNCCY 1 cut(s) 642
EcoRI GAATTC 2 cut(s) 334, 428
EcoRII CCWGG 1 cut(s) 215
Esp3I CGTCTC 1 cut(s) 74
FaeI CATG 1 cut(s) 490
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 1 cut(s) 978
FatI CATG 1 cut(s) 486
Fnu4HI GCNGC 4 cut(s) 119, 163, 184, 762
FokI GGATG 6 cut(s) 309, 635, 692, 707, 1061, 1067
Fsp4HI GCNGC 4 cut(s) 119, 163, 184, 762
FspBI CTAG 1 cut(s) 122
FspI TGCGCA 1 cut(s) 182
GlaI GCGC 1 cut(s) 182
GluI GCNGC 4 cut(s) 119, 163, 184, 762
GsaI CCCAGC 1 cut(s) 161
GsuI CTGGAG 1 cut(s) 999
HaeIII GGCC 5 cut(s) 162, 643, 869, 952, 975
HapII CCGG 4 cut(s) 38, 339, 676, 911
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 183
Hin1I GRCGYC 2 cut(s) 99, 233
Hin1II CATG 1 cut(s) 490
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HinfI GANTC 4 cut(s) 8, 252, 307, 1012
HpaII CCGG 4 cut(s) 38, 339, 676, 911
HphI GGTGA 3 cut(s) 529, 680, 908
Hpy166II GTNNAC 1 cut(s) 537
Hpy188I TCNGA 6 cut(s) 31, 312, 333, 736, 850, 984
Hpy188III TCNNGA 4 cut(s) 131, 249, 509, 881
Hpy8I GTNNAC 1 cut(s) 537
Hpy99I CGWCG 5 cut(s) 104, 137, 144, 238, 736
HpyAV CCTTC 2 cut(s) 662, 763
HpyCH4III ACNGT 5 cut(s) 153, 209, 319, 548, 774
HpyCH4IV ACGT 5 cut(s) 67, 142, 222, 233, 267
HpyCH4V TGCA 3 cut(s) 108, 761, 1043
HpyF10VI GCNNNNNNNGC 3 cut(s) 105, 440, 1025
HpyF3I CTNAG 1 cut(s) 463
HpySE526I ACGT 5 cut(s) 67, 142, 222, 233, 267
Hsp92I GRCGYC 2 cut(s) 99, 233
Hsp92II CATG 1 cut(s) 490
HspAI GCGC 1 cut(s) 181
Kzo9I GATC 3 cut(s) 31, 43, 359
LmnI GCTCC 2 cut(s) 502, 553
Lsp1109I GCAGC 3 cut(s) 130, 195, 773
LweI GCATC 3 cut(s) 748, 1083, 1089
MaeI CTAG 1 cut(s) 122
MaeII ACGT 5 cut(s) 67, 142, 222, 233, 267
MaeIII GTNAC 3 cut(s) 58, 167, 936
MalI GATC 3 cut(s) 33, 45, 361
MbiI CCGCTC 1 cut(s) 497
MboI GATC 3 cut(s) 31, 43, 359
MboII GAAGA 5 cut(s) 26, 80, 294, 392, 401
MflI RGATCY 1 cut(s) 359
MhlI GDGCHC 2 cut(s) 558, 911
MlsI TGGCCA 1 cut(s) 952
MluNI TGGCCA 1 cut(s) 952
MlyI GAGTC 1 cut(s) 1006
MmeI TCCRAC 2 cut(s) 524, 714
Mox20I TGGCCA 1 cut(s) 952
MscI TGGCCA 1 cut(s) 952
MseI TTAA 5 cut(s) 50, 452, 635, 843, 989
MslI CAYNNNNRTG 1 cut(s) 266
Msp20I TGGCCA 1 cut(s) 952
MspA1I CMGCKG 1 cut(s) 165
MspI CCGG 4 cut(s) 38, 339, 676, 911
MspR9I CCNGG 3 cut(s) 217, 677, 911
MvaI CCWGG 1 cut(s) 217
MwoI GCNNNNNNNGC 3 cut(s) 105, 440, 1025
NciI CCSGG 2 cut(s) 677, 911
NdeII GATC 3 cut(s) 31, 43, 359
NheI GCTAGC 1 cut(s) 121
NlaIII CATG 1 cut(s) 490
NlaIV GGNNCC 1 cut(s) 751
NmeAIII GCCGAG 2 cut(s) 212, 1001
NmuCI GTSAC 2 cut(s) 58, 936
NsbI TGCGCA 1 cut(s) 182
NspV TTCGAA 1 cut(s) 412
OliI CACNNNNGTG 1 cut(s) 266
PfeI GAWTC 3 cut(s) 8, 252, 307
PflMI CCANNNNNTGG 1 cut(s) 1022
PfoI TCCNGGA 1 cut(s) 675
PkrI GCNGC 4 cut(s) 120, 164, 185, 763
PleI GAGTC 1 cut(s) 1006
PpsI GAGTC 1 cut(s) 1006
PshBI ATTAAT 1 cut(s) 635
Psp6I CCWGG 1 cut(s) 215
PspFI CCCAGC 1 cut(s) 157
PspGI CCWGG 1 cut(s) 215
PspN4I GGNNCC 1 cut(s) 751
PspPI GGNCC 2 cut(s) 160, 642
PsuI RGATCY 1 cut(s) 359
RsaI GTAC 2 cut(s) 221, 279
RsaNI GTAC 2 cut(s) 220, 278
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 5 cut(s) 50, 452, 635, 843, 989
SatI GCNGC 4 cut(s) 119, 163, 184, 762
Sau3AI GATC 3 cut(s) 31, 43, 359
Sau96I GGNCC 2 cut(s) 160, 642
SchI GAGTC 1 cut(s) 1006
ScrFI CCNGG 3 cut(s) 217, 677, 911
SduI GDGCHC 2 cut(s) 558, 911
SfaNI GCATC 3 cut(s) 748, 1083, 1089
SfcI CTRYAG 1 cut(s) 423
SfuI TTCGAA 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 266
SsiI CCGC 5 cut(s) 163, 495, 540, 692, 1078
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 3 cut(s) 215, 675, 909
TaaI ACNGT 5 cut(s) 153, 209, 319, 548, 774
TaiI ACGT 5 cut(s) 70, 145, 225, 236, 270
TaqI TCGA 6 cut(s) 248, 305, 412, 474, 756, 880
TaqII GACCGA 1 cut(s) 153
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 3 cut(s) 8, 252, 307
Tru1I TTAA 5 cut(s) 50, 452, 635, 843, 989
Tru9I TTAA 5 cut(s) 50, 452, 635, 843, 989
TscAI CASTG 2 cut(s) 553, 779
TseFI GTSAC 2 cut(s) 58, 936
TseI GCWGC 3 cut(s) 118, 183, 761
Tsp45I GTSAC 2 cut(s) 58, 936
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 2 cut(s) 553, 779
Van91I CCANNNNNTGG 1 cut(s) 1022
VspI ATTAAT 1 cut(s) 635
XapI RAATTY 5 cut(s) 334, 428, 699, 899, 1037
XspI CTAG 1 cut(s) 122
ZraI GACGTC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.