RLG00000033780

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000673
Physical Location & Seq
Forward (+)
32049505 .. 32050737
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033780

Sequence Viewer

Length: 1233 bp
ATGATGAAAAATTCTAACAGAAAGAAAAACTCGTGTGATCAAACTCGAGCACATCGTATCACTCCAATCCGTTCAAACCATCGTTGTTGGTCTGATCTTCCAGTTGACCTGTCAAGATCAGTAGCTGAACGTCTCATATATCCTGATTTTCTTCGCTTTCGTGCGGTATGCAAAAACTGGCAGCGAGCTTTTTCCGACGACGATGACCTTCAGCGACGGGCAGTATTTTCTTTAATACGGGTTGACAGTTCTCATTACTTCCACTATACCAAAACCCAAACAGTTTGTACAGCGCTCTTCTTTGATTTGCTGTCCCCATTACAAGTCTTCCCTATCAGAAACATGGTACAAGAACAAGTGCAAAAAGAGGACCTCGTTTATCCAAGTTTTTGTTGTTCATACTCCGGTTGGTTGCTGTTGTTAAAATCACATCCGTCAGATTACTATAAAGAGATAAAGTCCAGATTCTTTGTTCATAATCCTTTTCGCAGCGAGACTGTGGAATTGCATCTGCCTCCAGTTTCGCGGCACTACCTTTCCAGAGAGGTAGAGGTAGCAGGCTTCTCGACCACTCCAACTTCTCTGGATTGTGTTATCTTTCTCCTGTGGAAAATTCAAAAATGCAATGGTTATGATTCTGATCCTAATTACGAGGAGTGGAATGAGTTCTTTATAACCACGTACAACAGAAAACATCCAAATCAAAAGTATAGAAGTTGGAGTGTGTATGAACTTCCTCGTCGCAATCCCAAAAATAAGGAGTGGAAGTTCATCAAGGACGTGGCTTACATGGATGGAAGTTTCTATTGTGCTGTGGATATGAATTCAAAGGTTGTATTGGGTACCTTCAATGTAGCACTTCAAGAGTGGGTTATGGTGATGAAGAGATCCTTGAGACTACCATCTCGTCCAGAACTCATGAACTTGGTTAAATCTGATGAAGGGCTACTATTGGCAGTTTGTTATAAGGAGCAAGATGGTGATGAATTTTTCTACCATATTTACCAGTTTGATTTGTCACGAAAAGAATGGACAAGAGTTAAAAGTTTGGGAAACCGAGTATTGATGTTGCATAGCCGTCCGTCAGTTGTGACTCCGGCGGTGGGGATGACCAGTAAATTAGCAAACATGATATTTGTCCGTGGCTTGAACTCGGACAGCTATGTTGCTTTTACTTGGGAAGTTGAAGGCGAATGGAGATGGCGGCAATTAGATAGCATTATATCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

411

Amino Acids

48.59

Weight (kDa)

9.01

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 30 - 68 1.7e-06 F-box domain
Beta-prop_KIB1-4 PF03478 122 - 380 2.5e-19 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 674, 966
Acc65I GGTACC 1 cut(s) 842
AccB1I GGYRCC 1 cut(s) 842
AccII CGCG 1 cut(s) 526
AciI CCGC 4 cut(s) 164, 526, 1100, 1204
AclWI GGATC 2 cut(s) 635, 882
AcsI RAATTY 4 cut(s) 10, 612, 823, 986
AcuI CTGAAG 1 cut(s) 194
AfaI GTAC 4 cut(s) 289, 348, 683, 844
AfeI AGCGCT 1 cut(s) 294
AfiI CCNNNNNNNGG 1 cut(s) 1103
AgsI TTSAA 7 cut(s) 75, 617, 828, 850, 863, 1150, 1187
AjiI CACGTC 1 cut(s) 781
AloI GAACNNNNNNTCC 2 cut(s) 752, 784
AluBI AGCT 3 cut(s) 125, 188, 1161
AluI AGCT 3 cut(s) 125, 188, 1161
Alw21I GWGCWC 1 cut(s) 52
Alw26I GTCTC 3 cut(s) 137, 488, 889
AlwI GGATC 2 cut(s) 635, 882
AlwNI CAGNNNCTG 1 cut(s) 125
Ama87I CYCGRG 1 cut(s) 45
Aor51HI AGCGCT 1 cut(s) 294
ApeKI GCWGC 2 cut(s) 181, 489
ApoI RAATTY 4 cut(s) 10, 612, 823, 986
Asp700I GAANNNNTTC 1 cut(s) 665
Asp718I GGTACC 1 cut(s) 842
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 370
AsuHPI GGTGA 2 cut(s) 889, 992
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 1 cut(s) 370
BanI GGYRCC 1 cut(s) 842
BauI CACGAG 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 52
BbvI GCAGC 2 cut(s) 193, 501
BccI CCATC 5 cut(s) 87, 788, 910, 971, 1194
BceAI ACGGC 1 cut(s) 1062
BcgI CGANNNNNNTGC 2 cut(s) 546, 580
BclI TGATCA 1 cut(s) 37
BcoDI GTCTC 3 cut(s) 137, 488, 889
BfoI RGCGCY 1 cut(s) 296
BisI GCNGC 4 cut(s) 182, 490, 527, 1205
BlsI GCNGC 4 cut(s) 183, 491, 528, 1206
Bme18I GGWCC 1 cut(s) 370
BmeT110I CYCGRG 1 cut(s) 45
BmgBI CACGTC 1 cut(s) 781
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 1 cut(s) 844
BmsI GCATC 1 cut(s) 517
BpiI GAAGAC 1 cut(s) 319
BpmI CTGGAG 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 913
BsaAI YACGTR 1 cut(s) 681
BsaBI GATNNNNATC 1 cut(s) 639
BsaJI CCNNGG 1 cut(s) 1141
BsaWI WCCGGW 1 cut(s) 404
BsaXI ACNNNNNCTCC 2 cut(s) 752, 782
Bsc4I CCNNNNNNNGG 1 cut(s) 1103
Bse1I ACTGG 5 cut(s) 101, 182, 518, 1006, 1113
Bse3DI GCAATG 1 cut(s) 631
Bse8I GATNNNNATC 1 cut(s) 639
BseDI CCNNGG 1 cut(s) 1141
BseGI GGATG 4 cut(s) 430, 694, 799, 1113
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 1 cut(s) 1103
BseMI GCAATG 1 cut(s) 631
BseNI ACTGG 5 cut(s) 101, 182, 518, 1006, 1113
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 2 cut(s) 193, 501
Bsh1236I CGCG 1 cut(s) 526
BshNI GGYRCC 1 cut(s) 842
BsiHKAI GWGCWC 1 cut(s) 52
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 2 cut(s) 405, 1097
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 1103
BsmAI GTCTC 3 cut(s) 137, 488, 889
BsmBI CGTCTC 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 298
BsoBI CYCGRG 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 52
Bsp1407I TGTACA 1 cut(s) 287
Bsp143I GATC 5 cut(s) 37, 94, 116, 640, 887
BspACI CCGC 4 cut(s) 164, 526, 1100, 1204
BspFNI CGCG 1 cut(s) 526
BspHI TCATGA 1 cut(s) 918
BspLI GGNNCC 1 cut(s) 844
BspPI GGATC 2 cut(s) 635, 882
BspQI GCTCTTC 1 cut(s) 302
BspT107I GGYRCC 1 cut(s) 842
BsrDI GCAATG 1 cut(s) 631
BsrGI TGTACA 1 cut(s) 287
BsrI ACTGG 5 cut(s) 101, 182, 518, 1006, 1113
BssECI CCNNGG 1 cut(s) 1141
BssMI GATC 5 cut(s) 37, 94, 116, 640, 887
BssSI CACGAG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 31
Bst4CI ACNGT 3 cut(s) 248, 283, 499
Bst6I CTCTTC 2 cut(s) 302, 878
BstAUI TGTACA 1 cut(s) 287
BstBAI YACGTR 1 cut(s) 681
BstC8I GCNNGC 2 cut(s) 186, 559
BstDEI CTNAG 1 cut(s) 1227
BstDSI CCRYGG 1 cut(s) 1141
BstF5I GGATG 4 cut(s) 430, 694, 799, 1113
BstFNI CGCG 1 cut(s) 526
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 5 cut(s) 40, 97, 119, 643, 890
BstMAI GTCTC 3 cut(s) 137, 488, 889
BstMBI GATC 5 cut(s) 37, 94, 116, 640, 887
BstUI CGCG 1 cut(s) 526
BstV1I GCAGC 2 cut(s) 193, 501
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 1 cut(s) 887
BstYI RGATCY 1 cut(s) 887
BtgI CCRYGG 1 cut(s) 1141
BtrI CACGTC 1 cut(s) 781
BtsCI GGATG 4 cut(s) 430, 694, 799, 1113
Cac8I GCNNGC 2 cut(s) 186, 559
CaiI CAGNNNCTG 1 cut(s) 125
CciI TCATGA 1 cut(s) 918
CfoI GCGC 1 cut(s) 295
Cfr13I GGNCC 1 cut(s) 370
Csp6I GTAC 4 cut(s) 288, 347, 682, 843
CviAII CATG 4 cut(s) 343, 790, 919, 1129
CviJI RGCY 8 cut(s) 125, 188, 561, 785, 946, 1077, 1146, 1161
CviKI_1 RGCY 8 cut(s) 125, 188, 561, 785, 946, 1077, 1146, 1161
CviQI GTAC 4 cut(s) 288, 347, 682, 843
DdeI CTNAG 1 cut(s) 1227
DpnI GATC 5 cut(s) 39, 96, 118, 642, 889
DpnII GATC 5 cut(s) 37, 94, 116, 640, 887
Eam1104I CTCTTC 2 cut(s) 302, 878
EarI CTCTTC 2 cut(s) 302, 878
Eco47I GGWCC 1 cut(s) 370
Eco47III AGCGCT 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 194
Eco88I CYCGRG 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 823
Esp3I CGTCTC 1 cut(s) 137
FaeI CATG 4 cut(s) 346, 793, 922, 1132
FalI AAGNNNNNCTT 2 cut(s) 875, 907
FaqI GGGAC 1 cut(s) 298
FatI CATG 4 cut(s) 342, 789, 918, 1128
FbaI TGATCA 1 cut(s) 37
Fnu4HI GCNGC 4 cut(s) 182, 490, 527, 1205
FokI GGATG 4 cut(s) 417, 681, 806, 1120
Fsp4HI GCNGC 4 cut(s) 182, 490, 527, 1205
GlaI GCGC 1 cut(s) 294
GluI GCNGC 4 cut(s) 182, 490, 527, 1205
GsuI CTGGAG 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 296
HapII CCGG 2 cut(s) 405, 1097
HhaI GCGC 1 cut(s) 295
Hin1II CATG 4 cut(s) 346, 793, 922, 1132
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 2 cut(s) 106, 244
HindII GTYRAC 2 cut(s) 106, 244
HinfI GANTC 3 cut(s) 465, 635, 1093
HpaII CCGG 2 cut(s) 405, 1097
HphI GGTGA 2 cut(s) 889, 992
Hpy166II GTNNAC 2 cut(s) 106, 244
Hpy188I TCNGA 7 cut(s) 94, 196, 338, 439, 640, 937, 1156
Hpy8I GTNNAC 2 cut(s) 106, 244
Hpy99I CGWCG 4 cut(s) 200, 203, 219, 744
HpyAV CCTTC 4 cut(s) 218, 856, 935, 1181
HpyCH4III ACNGT 3 cut(s) 248, 283, 499
HpyCH4IV ACGT 3 cut(s) 130, 680, 780
HpyCH4V TGCA 5 cut(s) 171, 361, 508, 624, 1072
HpyF3I CTNAG 1 cut(s) 1227
HpySE526I ACGT 3 cut(s) 130, 680, 780
Hsp92II CATG 4 cut(s) 346, 793, 922, 1132
HspAI GCGC 1 cut(s) 293
KpnI GGTACC 1 cut(s) 846
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 5 cut(s) 37, 94, 116, 640, 887
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 1 cut(s) 970
Lsp1109I GCAGC 2 cut(s) 193, 501
LweI GCATC 1 cut(s) 517
MaeII ACGT 3 cut(s) 130, 680, 780
MaeIII GTNAC 2 cut(s) 1017, 1090
MalI GATC 5 cut(s) 39, 96, 118, 642, 889
MboI GATC 5 cut(s) 37, 94, 116, 640, 887
MboII GAAGA 5 cut(s) 89, 143, 289, 319, 895
MflI RGATCY 1 cut(s) 887
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 8 cut(s) 10, 503, 612, 646, 823, 986, 1118, 1208
MlyI GAGTC 1 cut(s) 1087
MmeI TCCRAC 3 cut(s) 219, 599, 698
MnlI CCTC 7 cut(s) 361, 383, 525, 538, 544, 646, 747
MroXI GAANNNNTTC 1 cut(s) 665
MseI TTAA 4 cut(s) 233, 422, 930, 1041
MspI CCGG 2 cut(s) 405, 1097
MvnI CGCG 1 cut(s) 526
NdeII GATC 5 cut(s) 37, 94, 116, 640, 887
NlaIII CATG 4 cut(s) 346, 793, 922, 1132
NlaIV GGNNCC 1 cut(s) 844
NmuCI GTSAC 2 cut(s) 1017, 1090
PaeR7I CTCGAG 1 cut(s) 45
PagI TCATGA 1 cut(s) 918
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 52
PdmI GAANNNNTTC 1 cut(s) 665
PfeI GAWTC 2 cut(s) 465, 635
PkrI GCNGC 4 cut(s) 183, 491, 528, 1206
PleI GAGTC 1 cut(s) 1087
PpsI GAGTC 1 cut(s) 1087
Ppu21I YACGTR 1 cut(s) 681
PpuMI RGGWCCY 1 cut(s) 370
PsiI TTATAA 2 cut(s) 674, 966
Psp5II RGGWCCY 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 844
PspPI GGNCC 1 cut(s) 370
PspPPI RGGWCCY 1 cut(s) 370
PspXI VCTCGAGB 1 cut(s) 45
PstNI CAGNNNCTG 1 cut(s) 125
PsuI RGATCY 1 cut(s) 887
RsaI GTAC 4 cut(s) 289, 348, 683, 844
RsaNI GTAC 4 cut(s) 288, 347, 682, 843
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 4 cut(s) 233, 422, 930, 1041
SatI GCNGC 4 cut(s) 182, 490, 527, 1205
Sau3AI GATC 5 cut(s) 37, 94, 116, 640, 887
Sau96I GGNCC 1 cut(s) 370
SchI GAGTC 1 cut(s) 1087
SduI GDGCHC 1 cut(s) 52
SfaNI GCATC 1 cut(s) 517
Sfr274I CTCGAG 1 cut(s) 45
SinI GGWCC 1 cut(s) 370
SlaI CTCGAG 1 cut(s) 45
SmlI CTYRAG 2 cut(s) 45, 892
SmoI CTYRAG 2 cut(s) 45, 892
Sse9I AATT 8 cut(s) 10, 503, 612, 646, 823, 986, 1118, 1208
SsiI CCGC 4 cut(s) 164, 526, 1100, 1204
TaaI ACNGT 3 cut(s) 248, 283, 499
TaiI ACGT 3 cut(s) 133, 683, 783
TaqI TCGA 2 cut(s) 46, 566
TasI AATT 8 cut(s) 10, 503, 612, 646, 823, 986, 1118, 1208
TatI WGTACW 1 cut(s) 287
TauI GCSGC 2 cut(s) 529, 1207
TfiI GAWTC 2 cut(s) 465, 635
Tru1I TTAA 4 cut(s) 233, 422, 930, 1041
Tru9I TTAA 4 cut(s) 233, 422, 930, 1041
TseFI GTSAC 2 cut(s) 1017, 1090
TseI GCWGC 2 cut(s) 181, 489
Tsp45I GTSAC 2 cut(s) 1017, 1090
TspGWI ACGGA 4 cut(s) 59, 423, 1071, 1130
VpaK11BI GGWCC 1 cut(s) 370
XapI RAATTY 4 cut(s) 10, 612, 823, 986
XhoI CTCGAG 1 cut(s) 45
XmnI GAANNNNTTC 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.