RLG00000033754

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000673
Physical Location & Seq
Forward (+)
31786947 .. 31789594
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033754

Sequence Viewer

Length: 1212 bp
ATGACACAATCACACCAACACTGTTGGTCTGATCTTCCGGTCGATCTGTTAACAGAAGTGACTGAACGTCTCTCATACAGTGATGTTCTTCGCTTTAGCGTCGTATGCAAAAAATGGCAGCTAGCTTTTCCCGAAGACCGACGTGTAATACTGTTCCAGGCCCGCTGTTACAGCCGTAAGCGCCGCCGAGGTGCTATTGAAACCTACTGTGAAGCCAGATCAGAGTCACCAGCCTATACAAGGAGTAAAGATAAGACGTTCTTTGTCCATAATCCTCTTCGCAAAGTGACTATGGAATTGGAACTGCCTAAATCTAGTTGCTCAGAGGTTCAGAACACCGTCGTCGATCTGGCAACAGCCTCCTTTGCGGCTTCTCGGCCATCACCGCTGGCTAGTCCATCATCGAGCACAGGCAACCATTCCTGTCCCCATCAGAAGCAGCACCGGACCACCGTGCCAATCACTAAGTCAAATCACCGGACCACCGTGCCATTCCTGTCCCCATCAGAAGCACCACCGTGCCGATCACTAAGACAAACCACAAATCTGGAGATCAACGTCGACCTCAAACACGAAAGCCCGACCTTGACCCTATTCCAAATCCGATTAGACAGCCTGAGACGCCGAGACCACCTTGAAAGGGAGCCAACCCAAGATAAGGACAGTGTGATTAATAGTTACACTATTACAACATATAACCAACGAGATAGAACATGGAGTATCTATAAAATTCCACCTTACCGAGGCGAAATAAGAATGTGGTTCTACATAGGTGTTGAAATCAGGGACGTGGCTTGTGCGGATGGAATTTTCTATTTTCTTGTTGTTATCAACTCGTCGGAAATTATGTTGGGCACCTTCGATGCAGCACTTCAAAAGTGGGTTCTAATGGACAATCCCAGCTCCACACTGAAACTACCATATCGTCCGGTATATCTTTACCTTGTTAAATCTGATGGGGAGCTCTTATTGGCAGTCTATCATCAACAGCAAGGTAGTGGTGAACTTTTGTGCCCTGAGGTTTACCGGTTTGATTATTTGTCACAAAAGAATTGGACAAGAGTTAATAAATTAGGAGGCCGAGTTCTGATTTTGAGTCCCTCTTGGTCGATTTCGACTCCAGCTGTGGGGGTTACAAGTAAAGTTGCAAACATGATATATGCTGAGGATGCGGAGTGCATCCCGACATTGGAGAAGTACAACATTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

404

Amino Acids

46.2

Weight (kDa)

8.89

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 9 - 40 2.1e-08 F-box domain
F-box-like PF12937 9 - 41 2.4e-06 F-box-like
Beta-prop_KIB1-4 PF03478 173 - 390 4.1e-13 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 854
AccI GTMKAC 1 cut(s) 561
AciI CCGC 6 cut(s) 163, 184, 368, 386, 800, 1172
AcoI YGGCCR 1 cut(s) 377
AcsI RAATTY 2 cut(s) 729, 807
AcyI GRCGYC 1 cut(s) 622
AfaI GTAC 1 cut(s) 1199
AfiI CCNNNNNNNGG 6 cut(s) 240, 640, 658, 743, 1127, 1189
AflIII ACRYGT 1 cut(s) 142
AgeI ACCGGT 1 cut(s) 1026
AgsI TTSAA 4 cut(s) 200, 638, 779, 875
AhdI GACNNNNNGTC 1 cut(s) 66
AjiI CACGTC 2 cut(s) 143, 790
AjnI CCWGG 1 cut(s) 156
AleI CACNNNNGTG 1 cut(s) 517
AloI GAACNNNNNNTCC 2 cut(s) 1068, 1100
AluBI AGCT 5 cut(s) 121, 125, 903, 964, 1124
AluI AGCT 5 cut(s) 121, 125, 903, 964, 1124
Alw21I GWGCWC 2 cut(s) 410, 966
Alw26I GTCTC 3 cut(s) 74, 613, 621
AoxI GGCC 3 cut(s) 159, 377, 1078
ApeKI GCWGC 3 cut(s) 118, 439, 866
ApoI RAATTY 2 cut(s) 729, 807
AseI ATTAAT 1 cut(s) 672
AsiGI ACCGGT 1 cut(s) 1026
AspLEI GCGC 1 cut(s) 183
AspS9I GGNCC 3 cut(s) 160, 447, 480
AsuHPI GGTGA 4 cut(s) 219, 375, 467, 1013
AsuNHI GCTAGC 1 cut(s) 121
AvaII GGWCC 2 cut(s) 447, 480
AxyI CCTNAGG 1 cut(s) 1017
BaeGI GKGCMC 2 cut(s) 857, 1016
BanI GGYRCC 1 cut(s) 854
BanII GRGCYC 1 cut(s) 966
BbsI GAAGAC 1 cut(s) 141
Bbv12I GWGCWC 2 cut(s) 410, 966
BbvCI CCTCAGC 1 cut(s) 1164
BbvI GCAGC 3 cut(s) 130, 451, 878
BccI CCATC 6 cut(s) 388, 406, 438, 511, 797, 950
BceAI ACGGC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 158
BcoDI GTCTC 3 cut(s) 74, 613, 621
BfaI CTAG 3 cut(s) 122, 315, 393
BfoI RGCGCY 1 cut(s) 184
BisI GCNGC 5 cut(s) 119, 184, 369, 440, 867
BlsI GCNGC 5 cut(s) 120, 185, 370, 441, 868
Bme1390I CCNGG 1 cut(s) 158
Bme18I GGWCC 2 cut(s) 447, 480
BmeRI GACNNNNNGTC 1 cut(s) 66
BmgBI CACGTC 2 cut(s) 143, 790
BmgT120I GGNCC 3 cut(s) 160, 447, 480
BmiI GGNNCC 2 cut(s) 645, 856
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 3 cut(s) 853, 1159, 1188
BmtI GCTAGC 1 cut(s) 125
BpiI GAAGAC 1 cut(s) 141
BpmI CTGGAG 2 cut(s) 569, 1104
Bpu10I CCTNAGC 1 cut(s) 1164
BsaHI GRCGYC 1 cut(s) 622
BsaI GGTCTC 1 cut(s) 621
BsaJI CCNNGG 2 cut(s) 187, 742
BsaWI WCCGGW 5 cut(s) 37, 444, 477, 928, 1026
BsaXI ACNNNNNCTCC 2 cut(s) 1068, 1098
Bsc4I CCNNNNNNNGG 6 cut(s) 240, 640, 658, 743, 1127, 1189
Bse118I RCCGGY 1 cut(s) 1026
Bse21I CCTNAGG 1 cut(s) 1017
Bse3DI GCAATG 1 cut(s) 1203
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 2 cut(s) 187, 742
BseGI GGATG 3 cut(s) 808, 1174, 1179
BseLI CCNNNNNNNGG 6 cut(s) 240, 640, 658, 743, 1127, 1189
BseMI GCAATG 1 cut(s) 1203
BseMII CTCAG 4 cut(s) 336, 608, 1008, 1155
BseSI GKGCMC 2 cut(s) 857, 1016
BseXI GCAGC 3 cut(s) 130, 451, 878
BseYI CCCAGC 1 cut(s) 899
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 3 cut(s) 161, 379, 1080
BshNI GGYRCC 1 cut(s) 854
BshTI ACCGGT 1 cut(s) 1026
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 2 cut(s) 410, 966
BsiSI CCGG 5 cut(s) 38, 445, 478, 929, 1027
BslFI GGGAC 4 cut(s) 411, 484, 800, 1083
BslI CCNNNNNNNGG 6 cut(s) 240, 640, 658, 743, 1127, 1189
BsmAI GTCTC 3 cut(s) 74, 613, 621
BsmBI CGTCTC 2 cut(s) 74, 613
BsmFI GGGAC 4 cut(s) 411, 484, 800, 1083
BsnI GGCC 3 cut(s) 161, 379, 1080
Bso31I GGTCTC 1 cut(s) 621
Bsp1286I GDGCHC 4 cut(s) 410, 857, 966, 1016
Bsp143I GATC 6 cut(s) 31, 43, 218, 346, 524, 552
BspACI CCGC 6 cut(s) 163, 184, 368, 386, 800, 1172
BspANI GGCC 3 cut(s) 161, 379, 1080
BspCNI CTCAG 4 cut(s) 335, 609, 1009, 1156
BspLI GGNNCC 2 cut(s) 645, 856
BspOI GCTAGC 1 cut(s) 125
BspT107I GGYRCC 1 cut(s) 854
BspTNI GGTCTC 1 cut(s) 621
BsrDI GCAATG 1 cut(s) 1203
BsrFI RCCGGY 1 cut(s) 1026
BssAI RCCGGY 1 cut(s) 1026
BssECI CCNNGG 2 cut(s) 187, 742
BssMI GATC 6 cut(s) 31, 43, 218, 346, 524, 552
BssNI GRCGYC 1 cut(s) 622
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 9 cut(s) 23, 80, 153, 209, 340, 454, 487, 519, 665
Bst6I CTCTTC 1 cut(s) 282
BstACI GRCGYC 1 cut(s) 622
BstC8I GCNNGC 3 cut(s) 123, 163, 390
BstDEI CTNAG 6 cut(s) 322, 465, 530, 617, 1017, 1164
BstENI CCTNNNNNAGG 2 cut(s) 238, 741
BstF5I GGATG 3 cut(s) 808, 1174, 1179
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 6 cut(s) 34, 46, 221, 349, 527, 555
BstMAI GTCTC 3 cut(s) 74, 613, 621
BstMBI GATC 6 cut(s) 31, 43, 218, 346, 524, 552
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 7 cut(s) 105, 171, 180, 365, 385, 621, 1169
BstNI CCWGG 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 156
BstSLI GKGCMC 2 cut(s) 857, 1016
BstV1I GCAGC 3 cut(s) 130, 451, 878
BstV2I GAAGAC 1 cut(s) 141
BstXI CCANNNNNNTGG 1 cut(s) 547
Bsu36I CCTNAGG 1 cut(s) 1017
BsuRI GGCC 3 cut(s) 161, 379, 1080
BtrI CACGTC 2 cut(s) 143, 790
BtsCI GGATG 3 cut(s) 808, 1174, 1179
BtsIMutI CAGTG 4 cut(s) 19, 85, 670, 908
Cac8I GCNNGC 3 cut(s) 123, 163, 390
CfoI GCGC 1 cut(s) 183
Cfr10I RCCGGY 1 cut(s) 1026
Cfr13I GGNCC 3 cut(s) 160, 447, 480
CseI GACGC 2 cut(s) 88, 630
Csp6I GTAC 1 cut(s) 1198
CspAI ACCGGT 1 cut(s) 1026
CviAII CATG 2 cut(s) 714, 1153
CviQI GTAC 1 cut(s) 1198
DdeI CTNAG 6 cut(s) 322, 465, 530, 617, 1017, 1164
DpnI GATC 6 cut(s) 33, 45, 220, 348, 526, 554
DpnII GATC 6 cut(s) 31, 43, 218, 346, 524, 552
DriI GACNNNNNGTC 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 377
Eam1104I CTCTTC 1 cut(s) 282
Eam1105I GACNNNNNGTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 964
Eco24I GRGCYC 1 cut(s) 966
Eco31I GGTCTC 1 cut(s) 621
Eco47I GGWCC 2 cut(s) 447, 480
Eco53kI GAGCTC 1 cut(s) 964
Eco81I CCTNAGG 1 cut(s) 1017
EcoICRI GAGCTC 1 cut(s) 964
EcoNI CCTNNNNNAGG 2 cut(s) 238, 741
EcoRII CCWGG 1 cut(s) 156
EcoT38I GRGCYC 1 cut(s) 966
Esp3I CGTCTC 2 cut(s) 74, 613
FaeI CATG 2 cut(s) 717, 1156
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 4 cut(s) 411, 484, 800, 1083
FatI CATG 2 cut(s) 713, 1152
FauI CCCGC 1 cut(s) 170
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 5 cut(s) 119, 184, 369, 440, 867
FokI GGATG 3 cut(s) 815, 1166, 1181
FriOI GRGCYC 1 cut(s) 966
Fsp4HI GCNGC 5 cut(s) 119, 184, 369, 440, 867
FspBI CTAG 3 cut(s) 122, 315, 393
GlaI GCGC 1 cut(s) 182
GluI GCNGC 5 cut(s) 119, 184, 369, 440, 867
GsaI CCCAGC 1 cut(s) 903
GsuI CTGGAG 2 cut(s) 569, 1104
HaeII RGCGCY 1 cut(s) 184
HaeIII GGCC 3 cut(s) 161, 379, 1080
HapII CCGG 5 cut(s) 38, 445, 478, 929, 1027
HgaI GACGC 2 cut(s) 88, 630
HhaI GCGC 1 cut(s) 183
Hin1I GRCGYC 1 cut(s) 622
Hin1II CATG 2 cut(s) 717, 1156
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HincII GTYRAC 2 cut(s) 51, 562
HindII GTYRAC 2 cut(s) 51, 562
HinfI GANTC 3 cut(s) 224, 1096, 1117
HpaI GTTAAC 1 cut(s) 51
HpaII CCGG 5 cut(s) 38, 445, 478, 929, 1027
HphI GGTGA 4 cut(s) 219, 375, 467, 1013
Hpy166II GTNNAC 4 cut(s) 51, 562, 1004, 1024
Hpy188III TCNNGA 3 cut(s) 131, 548, 1183
Hpy8I GTNNAC 4 cut(s) 51, 562, 1004, 1024
Hpy99I CGWCG 6 cut(s) 104, 144, 344, 347, 563, 841
HpyAV CCTTC 1 cut(s) 868
HpyCH4III ACNGT 9 cut(s) 23, 80, 153, 209, 340, 454, 487, 519, 665
HpyCH4IV ACGT 5 cut(s) 67, 142, 257, 558, 789
HpyCH4V TGCA 4 cut(s) 108, 866, 1148, 1179
HpyF10VI GCNNNNNNNGC 7 cut(s) 105, 171, 180, 365, 385, 621, 1169
HpyF3I CTNAG 6 cut(s) 322, 465, 530, 617, 1017, 1164
HpySE526I ACGT 5 cut(s) 67, 142, 257, 558, 789
Hsp92I GRCGYC 1 cut(s) 622
Hsp92II CATG 2 cut(s) 717, 1156
HspAI GCGC 1 cut(s) 181
KspAI GTTAAC 1 cut(s) 51
Kzo9I GATC 6 cut(s) 31, 43, 218, 346, 524, 552
LmnI GCTCC 3 cut(s) 643, 908, 961
Lsp1109I GCAGC 3 cut(s) 130, 451, 878
LweI GCATC 3 cut(s) 853, 1159, 1188
MaeI CTAG 3 cut(s) 122, 315, 393
MaeII ACGT 5 cut(s) 67, 142, 257, 558, 789
MaeIII GTNAC 7 cut(s) 58, 167, 225, 286, 677, 1041, 1132
MalI GATC 6 cut(s) 33, 45, 220, 348, 526, 554
MboI GATC 6 cut(s) 31, 43, 218, 346, 524, 552
MboII GAAGA 4 cut(s) 26, 80, 146, 269
MhlI GDGCHC 4 cut(s) 410, 857, 966, 1016
MluCI AATT 6 cut(s) 296, 729, 807, 843, 1051, 1070
MlyI GAGTC 3 cut(s) 233, 1105, 1111
MmeI TCCRAC 1 cut(s) 819
MseI TTAA 5 cut(s) 50, 672, 948, 1065, 1210
MslI CAYNNNNRTG 1 cut(s) 517
MspA1I CMGCKG 3 cut(s) 165, 388, 1124
MspI CCGG 5 cut(s) 38, 445, 478, 929, 1027
MspR9I CCNGG 1 cut(s) 158
MvaI CCWGG 1 cut(s) 158
MwoI GCNNNNNNNGC 7 cut(s) 105, 171, 180, 365, 385, 621, 1169
NdeII GATC 6 cut(s) 31, 43, 218, 346, 524, 552
NheI GCTAGC 1 cut(s) 121
NlaIII CATG 2 cut(s) 717, 1156
NlaIV GGNNCC 2 cut(s) 645, 856
NmeAIII GCCGAG 4 cut(s) 212, 355, 650, 1106
NmuCI GTSAC 4 cut(s) 58, 225, 286, 1041
OliI CACNNNNGTG 1 cut(s) 517
PinAI ACCGGT 1 cut(s) 1026
PkrI GCNGC 5 cut(s) 120, 185, 370, 441, 868
PleI GAGTC 3 cut(s) 232, 1104, 1111
PpsI GAGTC 3 cut(s) 232, 1104, 1111
PshBI ATTAAT 1 cut(s) 672
Psp124BI GAGCTC 1 cut(s) 966
Psp6I CCWGG 1 cut(s) 156
PspFI CCCAGC 1 cut(s) 899
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 2 cut(s) 645, 856
PspPI GGNCC 3 cut(s) 160, 447, 480
PvuII CAGCTG 1 cut(s) 1124
RsaI GTAC 1 cut(s) 1199
RsaNI GTAC 1 cut(s) 1198
RseI CAYNNNNRTG 1 cut(s) 517
SacI GAGCTC 1 cut(s) 966
SalI GTCGAC 1 cut(s) 560
SaqAI TTAA 5 cut(s) 50, 672, 948, 1065, 1210
SatI GCNGC 5 cut(s) 119, 184, 369, 440, 867
Sau3AI GATC 6 cut(s) 31, 43, 218, 346, 524, 552
Sau96I GGNCC 3 cut(s) 160, 447, 480
SchI GAGTC 3 cut(s) 233, 1105, 1111
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 4 cut(s) 410, 857, 966, 1016
SfaNI GCATC 3 cut(s) 853, 1159, 1188
SinI GGWCC 2 cut(s) 447, 480
SmiMI CAYNNNNRTG 1 cut(s) 517
Sse9I AATT 6 cut(s) 296, 729, 807, 843, 1051, 1070
SsiI CCGC 6 cut(s) 163, 184, 368, 386, 800, 1172
SspMI CTAG 3 cut(s) 122, 315, 393
SstI GAGCTC 1 cut(s) 966
StyD4I CCNGG 1 cut(s) 156
TaaI ACNGT 9 cut(s) 23, 80, 153, 209, 340, 454, 487, 519, 665
TaiI ACGT 5 cut(s) 70, 145, 260, 561, 792
TaqI TCGA 7 cut(s) 42, 345, 404, 561, 861, 1109, 1115
TaqII GACCGA 1 cut(s) 153
TasI AATT 6 cut(s) 296, 729, 807, 843, 1051, 1070
TatI WGTACW 1 cut(s) 1197
TauI GCSGC 2 cut(s) 186, 371
Tru1I TTAA 5 cut(s) 50, 672, 948, 1065, 1210
Tru9I TTAA 5 cut(s) 50, 672, 948, 1065, 1210
TscAI CASTG 4 cut(s) 26, 85, 670, 915
TseFI GTSAC 4 cut(s) 58, 225, 286, 1041
TseI GCWGC 3 cut(s) 118, 439, 866
Tsp45I GTSAC 4 cut(s) 58, 225, 286, 1041
TspRI CASTG 4 cut(s) 26, 85, 670, 915
VpaK11BI GGWCC 2 cut(s) 447, 480
VspI ATTAAT 1 cut(s) 672
XagI CCTNNNNNAGG 2 cut(s) 238, 741
XapI RAATTY 2 cut(s) 729, 807
XmiI GTMKAC 1 cut(s) 561
XspI CTAG 3 cut(s) 122, 315, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.