Rroxscaffold_1G00043510

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000673
Physical Location & Seq
Reverse (-)
62020184 .. 62027517
7334 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043510.1

Sequence Viewer

Length: 846 bp
ATGCAAACATGTGACACCATCGCCGCCAGTTATCCAACAGCCACAATAACTGCTTGGGCCACCGATATTGGCTCAATTTCTCCAAGACACGTCTTGTCCGGCCAAAATGCTCATTTTGGCGAGACTGTGGAATTGCATGTGCCTCCATCAGTTTCGTGGCACTACGTTTCCAGAGAGGTAGAGGTAGCAGGCTTCTCGACCACTCCAACTTCTCTGGATTGTGTTATCTTTCTCCTGTGGAAAATTCATAAATGCAATGGTTATGATTCTGATCCTAATTACGAGGAGTGGGATGAGTTCTTTATAACCACATACAACAGAGAACATCGAAGCCAAAAGTATAGAAGTTGGAGTGTGCATGAACTTCCTCGTCGCAATCCCAAAAATAAGAAGTGGAAGTTCATCAAGGACGTGGCTTACATGGATGGAAGTTTCTATTGTGCTGTGGATATGAATTCAGAGGTTGTATTGGGTACCTTCAATCTAGCACTTCAAGAAGATCCCTTGAGACTACCATCTCGTCCAGAACTCATGAACTTGGTTAAATCTGATGAAGGGCTACTATTGACAGTTTGTTATAAGGAGCAAGATGGTGATGAATTTTTCTACCATATTTACCAGTTTGATTTGTCACGAAAAGAATGGACAAGAGTTAAAAGTTTAGGAAACCGAGTATTGATGTTGCATAGCCGTCCGTCAGTTGCGACTCCGGCGGTGGGGATGATCAGTAAATTCGCAAACATGATATTTGTCCGTGGCTTGAACTCTGACAGCTATGTTGCTTTTACTTGGGAAGTTGAAGGCGAATGGAGACGGCGGCAATTAGATAGCATTATATCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

32.51

Weight (kDa)

6.0

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 108 - 250 5e-13 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 305, 579
Acc65I GGTACC 1 cut(s) 473
AccB1I GGYRCC 1 cut(s) 473
AciI CCGC 3 cut(s) 24, 713, 817
AclWI GGATC 2 cut(s) 266, 494
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 4 cut(s) 243, 454, 599, 731
AfaI GTAC 1 cut(s) 475
AfiI CCNNNNNNNGG 1 cut(s) 716
AflIII ACRYGT 2 cut(s) 8, 88
AgsI TTSAA 4 cut(s) 481, 494, 763, 800
AjiI CACGTC 2 cut(s) 91, 412
AluBI AGCT 1 cut(s) 774
AluI AGCT 1 cut(s) 774
Alw26I GTCTC 3 cut(s) 116, 502, 805
AlwI GGATC 2 cut(s) 266, 494
AoxI GGCC 2 cut(s) 57, 100
ApoI RAATTY 4 cut(s) 243, 454, 599, 731
Asp718I GGTACC 1 cut(s) 473
AspS9I GGNCC 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 605
BanI GGYRCC 1 cut(s) 473
BccI CCATC 5 cut(s) 26, 154, 419, 523, 584
BceAI ACGGC 2 cut(s) 675, 830
BcgI CGANNNNNNTGC 2 cut(s) 177, 211
BclI TGATCA 1 cut(s) 723
BcoDI GTCTC 3 cut(s) 116, 502, 805
BfaI CTAG 1 cut(s) 485
BisI GCNGC 2 cut(s) 24, 818
BlsI GCNGC 2 cut(s) 25, 819
BmgBI CACGTC 2 cut(s) 91, 412
BmgT120I GGNCC 1 cut(s) 57
BmiI GGNNCC 1 cut(s) 475
BpuEI CTTGAG 1 cut(s) 526
BsaBI GATNNNNATC 1 cut(s) 270
BsaJI CCNNGG 1 cut(s) 754
Bsc4I CCNNNNNNNGG 1 cut(s) 716
Bse1I ACTGG 2 cut(s) 27, 619
Bse3DI GCAATG 1 cut(s) 262
Bse8I GATNNNNATC 1 cut(s) 270
BseDI CCNNGG 1 cut(s) 754
BseGI GGATG 3 cut(s) 298, 430, 726
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMI GCAATG 1 cut(s) 262
BseNI ACTGG 2 cut(s) 27, 619
BseRI GAGGAG 1 cut(s) 299
BshFI GGCC 2 cut(s) 59, 102
BshNI GGYRCC 1 cut(s) 473
BsiSI CCGG 2 cut(s) 99, 710
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 3 cut(s) 116, 502, 805
BsmBI CGTCTC 1 cut(s) 805
BsnI GGCC 2 cut(s) 59, 102
Bsp143I GATC 3 cut(s) 271, 499, 723
BspACI CCGC 3 cut(s) 24, 713, 817
BspANI GGCC 2 cut(s) 59, 102
BspHI TCATGA 1 cut(s) 531
BspLI GGNNCC 1 cut(s) 475
BspPI GGATC 2 cut(s) 266, 494
BspT107I GGYRCC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 2 cut(s) 27, 619
BssECI CCNNGG 1 cut(s) 754
BssMI GATC 3 cut(s) 271, 499, 723
Bst4CI ACNGT 2 cut(s) 127, 571
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 1 cut(s) 840
BstDSI CCRYGG 1 cut(s) 754
BstF5I GGATG 3 cut(s) 298, 430, 726
BstKTI GATC 3 cut(s) 274, 502, 726
BstMAI GTCTC 3 cut(s) 116, 502, 805
BstMBI GATC 3 cut(s) 271, 499, 723
BstMWI GCNNNNNNNGC 1 cut(s) 710
BstNSI RCATGY 2 cut(s) 12, 140
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BsuRI GGCC 2 cut(s) 59, 102
BtgI CCRYGG 1 cut(s) 754
BtgZI GCGATG 1 cut(s) 4
BtrI CACGTC 2 cut(s) 91, 412
BtsCI GGATG 3 cut(s) 298, 430, 726
Cac8I GCNNGC 1 cut(s) 190
CciI TCATGA 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 57
Csp6I GTAC 1 cut(s) 474
CspCI CAANNNNNGTGG 2 cut(s) 49, 84
CviAII CATG 6 cut(s) 9, 137, 359, 421, 532, 742
CviQI GTAC 1 cut(s) 474
DdeI CTNAG 1 cut(s) 840
DpnI GATC 3 cut(s) 273, 501, 725
DpnII GATC 3 cut(s) 271, 499, 723
EaeI YGGCCR 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 454
Esp3I CGTCTC 1 cut(s) 805
FaeI CATG 6 cut(s) 12, 140, 362, 424, 535, 745
FatI CATG 6 cut(s) 8, 136, 358, 420, 531, 741
FbaI TGATCA 1 cut(s) 723
Fnu4HI GCNGC 2 cut(s) 24, 818
FokI GGATG 3 cut(s) 305, 437, 733
Fsp4HI GCNGC 2 cut(s) 24, 818
FspBI CTAG 1 cut(s) 485
GluI GCNGC 2 cut(s) 24, 818
HaeIII GGCC 2 cut(s) 59, 102
HapII CCGG 2 cut(s) 99, 710
Hin1II CATG 6 cut(s) 12, 140, 362, 424, 535, 745
HinfI GANTC 2 cut(s) 266, 706
HpaII CCGG 2 cut(s) 99, 710
HphI GGTGA 1 cut(s) 605
Hpy188I TCNGA 4 cut(s) 271, 460, 550, 769
Hpy188III TCNNGA 7 cut(s) 171, 196, 215, 494, 524, 532, 633
Hpy99I CGWCG 1 cut(s) 375
HpyAV CCTTC 3 cut(s) 487, 548, 794
HpyCH4III ACNGT 2 cut(s) 127, 571
HpyCH4IV ACGT 3 cut(s) 90, 165, 411
HpyCH4V TGCA 5 cut(s) 4, 136, 255, 358, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 710
HpyF3I CTNAG 1 cut(s) 840
HpySE526I ACGT 3 cut(s) 90, 165, 411
Hsp92II CATG 6 cut(s) 12, 140, 362, 424, 535, 745
KpnI GGTACC 1 cut(s) 477
Ksp22I TGATCA 1 cut(s) 723
Kzo9I GATC 3 cut(s) 271, 499, 723
LmnI GCTCC 1 cut(s) 583
LpnPI CCDG 9 cut(s) 40, 112, 174, 184, 200, 248, 537, 632, 723
MaeI CTAG 1 cut(s) 485
MaeII ACGT 3 cut(s) 90, 165, 411
MaeIII GTNAC 2 cut(s) 11, 630
MalI GATC 3 cut(s) 273, 501, 725
MboI GATC 3 cut(s) 271, 499, 723
MboII GAAGA 1 cut(s) 509
MflI RGATCY 1 cut(s) 499
MluCI AATT 8 cut(s) 75, 131, 243, 277, 454, 599, 731, 821
MlyI GAGTC 1 cut(s) 700
MmeI TCCRAC 3 cut(s) 59, 230, 329
MnlI CCTC 6 cut(s) 153, 169, 175, 277, 378, 454
MseI TTAA 2 cut(s) 543, 654
MspI CCGG 2 cut(s) 99, 710
MwoI GCNNNNNNNGC 1 cut(s) 710
NdeII GATC 3 cut(s) 271, 499, 723
NlaIII CATG 6 cut(s) 12, 140, 362, 424, 535, 745
NlaIV GGNNCC 1 cut(s) 475
NmuCI GTSAC 2 cut(s) 11, 630
NspI RCATGY 2 cut(s) 12, 140
PagI TCATGA 1 cut(s) 531
PciI ACATGT 1 cut(s) 8
PfeI GAWTC 1 cut(s) 266
PkrI GCNGC 2 cut(s) 25, 819
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
PscI ACATGT 1 cut(s) 8
PsiI TTATAA 2 cut(s) 305, 579
PspN4I GGNNCC 1 cut(s) 475
PspPI GGNCC 1 cut(s) 57
PsuI RGATCY 1 cut(s) 499
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
SaqAI TTAA 2 cut(s) 543, 654
SatI GCNGC 2 cut(s) 24, 818
Sau3AI GATC 3 cut(s) 271, 499, 723
Sau96I GGNCC 1 cut(s) 57
SchI GAGTC 1 cut(s) 700
SetI ASST 8 cut(s) 93, 168, 180, 186, 414, 465, 479, 776
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 8 cut(s) 75, 131, 243, 277, 454, 599, 731, 821
SsiI CCGC 3 cut(s) 24, 713, 817
SspMI CTAG 1 cut(s) 485
TaaI ACNGT 2 cut(s) 127, 571
TaiI ACGT 3 cut(s) 93, 168, 414
TaqI TCGA 2 cut(s) 197, 328
TasI AATT 8 cut(s) 75, 131, 243, 277, 454, 599, 731, 821
TauI GCSGC 2 cut(s) 26, 820
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 2 cut(s) 543, 654
Tru9I TTAA 2 cut(s) 543, 654
TseFI GTSAC 2 cut(s) 11, 630
Tsp45I GTSAC 2 cut(s) 11, 630
TspDTI ATGAA 7 cut(s) 236, 375, 391, 467, 548, 567, 612
TspGWI ACGGA 2 cut(s) 684, 743
XapI RAATTY 4 cut(s) 243, 454, 599, 731
XceI RCATGY 2 cut(s) 12, 140
XcmI CCANNNNNNNNNTGG 1 cut(s) 153
XspI CTAG 1 cut(s) 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.