RchiOBHm_Chr6g0295501

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000673
Physical Location & Seq
Forward (+)
57489472 .. 57490635
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26518

Sequence Viewer

Length: 1164 bp
ATGTCGAGCTATACCAAGGAAGCTGTTATTGCTCCTCGAATTTTCTCCTGTTGCCTCGAGCTGAGGACGAGTTCTAAAGAAGAAGAAGAATTGTTGGTCGAAGAAGAAGAAGAAGAAGGATGTTTCAATAATAATAACAACTTGATCCAGAACACAGGCAATGAACATTCTTTTTGGTCCGACCTTCCAATCGATATCTTATGCGAAATCGCCGATCGTCTCCCATATTGGGATCTTGTTCGTTTTGCTGCCGTATGCAGGACCTGGAGAGTTATCATATATGGGGTTCATCTTCCACAACGCTCCACATTGCTTGTGCCGTACCTGATGAGCTATACATGTATTCGCGATCGTAAACACATAGAATTCGTAGACCTGCAAGGTGAAACTTACAAATACACAGTTGGGAGAATACTCTTCGACGATACCAGACTCTATGCTTCAAAATATGGTTGGTTGCTGTTGAAAAAATATGAGCGACCCACAGAACAATTCTATCTCTACAATCCTTTTTGGAATGATGTTATACGACTGCCCCGGTTAGATTTGGAGTTGGATTTTCAGGTAGTATTAGCAACATTCACGACCCCTCCAATCTATGCCGACTGTGTTATCTTCGTCATTTCCAGAAGGTATGGATATACTGATGATCACATCATAAACGCATGTAAGCTCCGAGATAGAAGATGGAGTGTTCATAAAACTAGATATCAAGGTTCTGTTGAAGGAGTTACTTACCTAGATGGAATTTTCTATTGTGTTATTCCACAGATAATTGATGGTTCTTTGTCACTTTCGATCAATACCTTCAATATAGCACTCCAAGACTGGGTCATGGACTATGCCTCCATAACACTAGAACACCATACCCAAAGCACAGCCTTTTGCGTGGTTGAGTCGGATGGGAAATTATTATTAGCAGTTTCTGATGAACTAGTACTTCATGGTACCCGCAATTGGTTTATATACCAGTTTGATTGGTTACGAAAATATTGGACTAGGGTTCAAAGTTTGGGGAATCAGGTATTGCTTCTAAGTCGCTCATCATCAATTGCATGTCCAACATTGGGGAGGGATAGTAAATTTTCAAACATGATATTTCATCTTAACCTGGGCAAGGAGCCTTTTACTTTCAGTGGTGATGGTGTCTGGAGAAGGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

387

Amino Acids

45.3

Weight (kDa)

5.66

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 59 - 96 1.6e-10 F-box domain
F-box-like PF12937 59 - 95 7.3e-09 F-box-like
Beta-prop_KIB1-4 PF03478 129 - 357 4e-24 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 384
Acc65I GGTACC 1 cut(s) 947
AccB1I GGYRCC 1 cut(s) 947
AccI GTMKAC 1 cut(s) 372
AccII CGCG 1 cut(s) 348
AciI CCGC 1 cut(s) 952
AclWI GGATC 2 cut(s) 139, 240
AcsI RAATTY 4 cut(s) 39, 365, 747, 1082
AfaI GTAC 3 cut(s) 323, 939, 949
AfiI CCNNNNNNNGG 6 cut(s) 229, 258, 829, 957, 1067, 1117
AflIII ACRYGT 1 cut(s) 338
AgsI TTSAA 7 cut(s) 127, 444, 466, 725, 811, 1007, 1089
AhlI ACTAGT 1 cut(s) 934
AjnI CCWGG 2 cut(s) 263, 1110
AluBI AGCT 5 cut(s) 9, 23, 61, 333, 673
AluI AGCT 5 cut(s) 9, 23, 61, 333, 673
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 2 cut(s) 139, 240
AlwNI CAGNNNCTG 2 cut(s) 264, 926
Ama87I CYCGRG 1 cut(s) 56
AoxI GGCC 1 cut(s) 1157
ApeKI GCWGC 1 cut(s) 248
ApoI RAATTY 4 cut(s) 39, 365, 747, 1082
Asp718I GGTACC 1 cut(s) 947
AspS9I GGNCC 3 cut(s) 177, 261, 1158
AsuC2I CCSGG 1 cut(s) 538
AsuHPI GGTGA 2 cut(s) 395, 1151
AvaI CYCGRG 1 cut(s) 56
AvaII GGWCC 2 cut(s) 177, 261
BanI GGYRCC 1 cut(s) 947
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 235
BccI CCATC 5 cut(s) 681, 737, 773, 896, 1136
BceAI ACGGC 2 cut(s) 236, 304
BciT130I CCWGG 2 cut(s) 265, 1112
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 1 cut(s) 538
BcoDI GTCTC 1 cut(s) 224
BcuI ACTAGT 1 cut(s) 934
BfaI CTAG 5 cut(s) 705, 740, 857, 935, 999
BfuAI ACCTGC 1 cut(s) 384
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
BmcAI AGTACT 1 cut(s) 939
Bme1390I CCNGG 3 cut(s) 265, 538, 1112
Bme18I GGWCC 2 cut(s) 177, 261
BmeT110I CYCGRG 1 cut(s) 56
BmgT120I GGNCC 3 cut(s) 177, 261, 1158
BmiI GGNNCC 2 cut(s) 949, 1122
BmrFI CCNGG 3 cut(s) 265, 538, 1112
BmrI ACTGGG 1 cut(s) 838
BmuI ACTGGG 1 cut(s) 838
BpmI CTGGAG 1 cut(s) 286
Bpu10I CCTNAGC 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 538
Bsa29I ATCGAT 1 cut(s) 192
BsaJI CCNNGG 3 cut(s) 15, 536, 1111
BsaXI ACNNNNNCTCC 6 cut(s) 574, 604, 830, 860, 1063, 1093
Bsc4I CCNNNNNNNGG 6 cut(s) 229, 258, 829, 957, 1067, 1117
Bse1I ACTGG 2 cut(s) 833, 970
Bse3DI GCAATG 2 cut(s) 166, 308
BseBI CCWGG 2 cut(s) 265, 1112
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 3 cut(s) 15, 536, 1111
BseGI GGATG 2 cut(s) 125, 907
BseLI CCNNNNNNNGG 6 cut(s) 229, 258, 829, 957, 1067, 1117
BseMI GCAATG 2 cut(s) 166, 308
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 2 cut(s) 833, 970
BseRI GAGGAG 1 cut(s) 24
BseXI GCAGC 1 cut(s) 235
Bsh1236I CGCG 1 cut(s) 348
Bsh1285I CGRYCG 2 cut(s) 217, 352
BshFI GGCC 1 cut(s) 1159
BshNI GGYRCC 1 cut(s) 947
BshVI ATCGAT 1 cut(s) 192
BsiEI CGRYCG 2 cut(s) 217, 352
BsiHKCI CYCGRG 1 cut(s) 56
BsiSI CCGG 1 cut(s) 538
BslI CCNNNNNNNGG 6 cut(s) 229, 258, 829, 957, 1067, 1117
BsmAI GTCTC 1 cut(s) 224
BsmBI CGTCTC 1 cut(s) 224
BsnI GGCC 1 cut(s) 1159
BsoBI CYCGRG 1 cut(s) 56
Bsp143I GATC 6 cut(s) 144, 214, 232, 349, 649, 798
Bsp68I TCGCGA 1 cut(s) 348
BspACI CCGC 1 cut(s) 952
BspANI GGCC 1 cut(s) 1159
BspCNI CTCAG 1 cut(s) 54
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 2 cut(s) 949, 1122
BspMI ACCTGC 1 cut(s) 384
BspPI GGATC 2 cut(s) 139, 240
BspT107I GGYRCC 1 cut(s) 947
BsrDI GCAATG 2 cut(s) 166, 308
BsrI ACTGG 2 cut(s) 833, 970
BssECI CCNNGG 3 cut(s) 15, 536, 1111
BssMI GATC 6 cut(s) 144, 214, 232, 349, 649, 798
BssT1I CCWWGG 1 cut(s) 15
Bst2UI CCWGG 2 cut(s) 265, 1112
Bst4CI ACNGT 2 cut(s) 403, 608
Bst6I CTCTTC 1 cut(s) 422
BstDEI CTNAG 2 cut(s) 62, 1034
BstENI CCTNNNNNAGG 1 cut(s) 1115
BstF5I GGATG 2 cut(s) 125, 907
BstFNI CGCG 1 cut(s) 348
BstKTI GATC 6 cut(s) 147, 217, 235, 352, 652, 801
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 6 cut(s) 144, 214, 232, 349, 649, 798
BstMCI CGRYCG 2 cut(s) 217, 352
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstNI CCWGG 2 cut(s) 265, 1112
BstNSI RCATGY 3 cut(s) 342, 669, 1059
BstSCI CCNGG 3 cut(s) 263, 536, 1110
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 1 cut(s) 235
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 1 cut(s) 1159
BsuTUI ATCGAT 1 cut(s) 192
BtsCI GGATG 2 cut(s) 125, 907
BtsIMutI CAGTG 1 cut(s) 1141
BtuMI TCGCGA 1 cut(s) 348
BveI ACCTGC 1 cut(s) 384
CaiI CAGNNNCTG 2 cut(s) 264, 926
Cfr13I GGNCC 3 cut(s) 177, 261, 1158
ClaI ATCGAT 1 cut(s) 192
Csp6I GTAC 3 cut(s) 322, 938, 948
CspCI CAANNNNNGTGG 2 cut(s) 295, 330
CviAII CATG 6 cut(s) 339, 666, 835, 944, 1056, 1093
CviJI RGCY 8 cut(s) 9, 23, 61, 333, 673, 881, 1123, 1159
CviKI_1 RGCY 8 cut(s) 9, 23, 61, 333, 673, 881, 1123, 1159
CviQI GTAC 3 cut(s) 322, 938, 948
DdeI CTNAG 2 cut(s) 62, 1034
DpnI GATC 6 cut(s) 146, 216, 234, 351, 651, 800
DpnII GATC 6 cut(s) 144, 214, 232, 349, 649, 798
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
Eco130I CCWWGG 1 cut(s) 15
Eco32I GATATC 2 cut(s) 196, 710
Eco47I GGWCC 2 cut(s) 177, 261
Eco88I CYCGRG 1 cut(s) 56
EcoNI CCTNNNNNAGG 1 cut(s) 1115
EcoO109I RGGNCCY 2 cut(s) 261, 1158
EcoRI GAATTC 1 cut(s) 365
EcoRII CCWGG 2 cut(s) 263, 1110
EcoRV GATATC 2 cut(s) 196, 710
EcoT14I CCWWGG 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 15
Esp3I CGTCTC 1 cut(s) 224
FaeI CATG 6 cut(s) 342, 669, 838, 947, 1059, 1096
FatI CATG 6 cut(s) 338, 665, 834, 943, 1055, 1092
FauI CCCGC 1 cut(s) 959
FbaI TGATCA 1 cut(s) 649
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 1 cut(s) 249
FokI GGATG 2 cut(s) 132, 914
Fsp4HI GCNGC 1 cut(s) 249
FspBI CTAG 5 cut(s) 705, 740, 857, 935, 999
GluI GCNGC 1 cut(s) 249
GsuI CTGGAG 1 cut(s) 286
HaeIII GGCC 1 cut(s) 1159
HapII CCGG 1 cut(s) 538
Hin1II CATG 6 cut(s) 342, 669, 838, 947, 1059, 1096
HinfI GANTC 3 cut(s) 432, 896, 1018
HpaII CCGG 1 cut(s) 538
HphI GGTGA 2 cut(s) 395, 1151
Hpy166II GTNNAC 2 cut(s) 356, 373
Hpy188I TCNGA 4 cut(s) 181, 677, 901, 928
Hpy188III TCNNGA 5 cut(s) 148, 347, 583, 627, 1150
Hpy8I GTNNAC 2 cut(s) 356, 373
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 6 cut(s) 110, 194, 624, 719, 817, 1149
HpyCH4III ACNGT 2 cut(s) 403, 608
HpyCH4V TGCA 3 cut(s) 258, 379, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 2 cut(s) 62, 1034
Hsp92II CATG 6 cut(s) 342, 669, 838, 947, 1059, 1096
KpnI GGTACC 1 cut(s) 951
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 6 cut(s) 144, 214, 232, 349, 649, 798
LmnI GCTCC 4 cut(s) 37, 308, 678, 1120
Lsp1109I GCAGC 1 cut(s) 235
MaeI CTAG 5 cut(s) 705, 740, 857, 935, 999
MaeIII GTNAC 3 cut(s) 730, 789, 981
MalI GATC 6 cut(s) 146, 216, 234, 351, 651, 800
MboI GATC 6 cut(s) 144, 214, 232, 349, 649, 798
MfeI CAATTG 2 cut(s) 955, 1050
MflI RGATCY 1 cut(s) 232
MlyI GAGTC 2 cut(s) 426, 905
MmeI TCCRAC 4 cut(s) 204, 534, 879, 1085
MnlI CCTC 6 cut(s) 45, 57, 65, 600, 856, 1065
MseI TTAA 1 cut(s) 1107
MspI CCGG 1 cut(s) 538
MspR9I CCNGG 3 cut(s) 265, 538, 1112
MunI CAATTG 2 cut(s) 955, 1050
MvaI CCWGG 2 cut(s) 265, 1112
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 1 cut(s) 29
NciI CCSGG 1 cut(s) 538
NdeII GATC 6 cut(s) 144, 214, 232, 349, 649, 798
NlaIII CATG 6 cut(s) 342, 669, 838, 947, 1059, 1096
NlaIV GGNNCC 2 cut(s) 949, 1122
NmuCI GTSAC 1 cut(s) 789
NruI TCGCGA 1 cut(s) 348
NspI RCATGY 3 cut(s) 342, 669, 1059
PaeR7I CTCGAG 1 cut(s) 56
PciI ACATGT 1 cut(s) 338
PfeI GAWTC 1 cut(s) 1018
PflFI GACNNNGTC 1 cut(s) 830
PkrI GCNGC 1 cut(s) 250
Ple19I CGATCG 2 cut(s) 217, 352
PleI GAGTC 2 cut(s) 426, 904
PpsI GAGTC 2 cut(s) 426, 904
PpuMI RGGWCCY 1 cut(s) 261
PscI ACATGT 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 263, 1110
PspGI CCWGG 2 cut(s) 263, 1110
PspN4I GGNNCC 2 cut(s) 949, 1122
PspPI GGNCC 3 cut(s) 177, 261, 1158
PspPPI RGGWCCY 1 cut(s) 261
PspXI VCTCGAGB 1 cut(s) 56
PstNI CAGNNNCTG 2 cut(s) 264, 926
PsuI RGATCY 1 cut(s) 232
PsyI GACNNNGTC 1 cut(s) 830
PvuI CGATCG 2 cut(s) 217, 352
RruI TCGCGA 1 cut(s) 348
RsaI GTAC 3 cut(s) 323, 939, 949
RsaNI GTAC 3 cut(s) 322, 938, 948
SaqAI TTAA 1 cut(s) 1107
SatI GCNGC 1 cut(s) 249
Sau3AI GATC 6 cut(s) 144, 214, 232, 349, 649, 798
Sau96I GGNCC 3 cut(s) 177, 261, 1158
ScaI AGTACT 1 cut(s) 939
SchI GAGTC 2 cut(s) 426, 905
ScrFI CCNGG 3 cut(s) 265, 538, 1112
Sfr274I CTCGAG 1 cut(s) 56
SinI GGWCC 2 cut(s) 177, 261
SlaI CTCGAG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SpeI ACTAGT 1 cut(s) 934
SsiI CCGC 1 cut(s) 952
SspI AATATT 1 cut(s) 992
SspMI CTAG 5 cut(s) 705, 740, 857, 935, 999
StyD4I CCNGG 3 cut(s) 263, 536, 1110
StyI CCWWGG 1 cut(s) 15
TaaI ACNGT 2 cut(s) 403, 608
TaqI TCGA 7 cut(s) 5, 37, 57, 99, 192, 420, 797
TatI WGTACW 1 cut(s) 937
TfiI GAWTC 1 cut(s) 1018
Tru1I TTAA 1 cut(s) 1107
Tru9I TTAA 1 cut(s) 1107
TscAI CASTG 1 cut(s) 1141
TseFI GTSAC 1 cut(s) 789
TseI GCWGC 1 cut(s) 248
Tsp45I GTSAC 1 cut(s) 789
TspDTI ATGAA 6 cut(s) 177, 278, 686, 932, 945, 1091
TspRI CASTG 1 cut(s) 1141
Tth111I GACNNNGTC 1 cut(s) 830
VpaK11BI GGWCC 2 cut(s) 177, 261
XagI CCTNNNNNAGG 1 cut(s) 1115
XapI RAATTY 4 cut(s) 39, 365, 747, 1082
XceI RCATGY 3 cut(s) 342, 669, 1059
XhoI CTCGAG 1 cut(s) 56
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 5 cut(s) 705, 740, 857, 935, 999
ZrmI AGTACT 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.