Rmu_sc0004190.1_g000002

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004190.1
OG0000673
Physical Location & Seq
Forward (+)
1403 .. 2506
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004190.1_g000002.1.cds

Sequence Viewer

Length: 915 bp
atgacacaatcacaccaacactgttggtctgatcttccggtcgatctgttaacagaagtgactgaacgtctatcataccatgatgctcttcgctttagcgtcatatgcaaacaatggcagctagctattcccgacgaccgacgtgtaatactgttgcagggccgctgttacagccagtcaccagcctatacaaagagtaaagataagacgttctttgttcataatcctcttcgcaaagtgactatggaattggaactgcctccatttagttgcgcaaaggttcaggtagcaaccttgacgaccactccaacttctcccgattgtattatttttatcctatggaaaaccaattttagtgtaattaatatttacagtattacaacatataaccaacgagatagaacatggagtatctataatattccaccttaccgaggcggaattagaacctcattctacataggtctccatttggatggtgcaatcaagggcgtggcttgtgcggatggaattttctattttcttgttgttaccaactcgtcggaaattatgttgggcaccttcgatgcagcacttcaaaagtgggttctaaaggacaagccccgctccacactgaaactaccatatcgtctggtacatctttacctggttaaatctaatggggagctcttattggccgtctatcatcaacagcaaggtagtggtgaacttttgtgccctgaggtttaccggtttgattatttgtcacaaaagaattggacaagagttaataatttaggaggccgagttctgattttgagtccctcatgttcgatttcgactccagcagtgggggttacaagtaaatttgcaaacatgatatatgctgaggatgtggagtgcatcccgacattggagaagtacaacattgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

304

Amino Acids

34.62

Weight (kDa)

8.76

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 274
AccB1I GGYRCC 1 cut(s) 557
AccBSI CCGCTC 1 cut(s) 606
AciI CCGC 4 cut(s) 163, 438, 503, 604
AcoI YGGCCR 1 cut(s) 675
AcsI RAATTY 2 cut(s) 510, 845
AfaI GTAC 2 cut(s) 636, 902
AfiI CCNNNNNNNGG 3 cut(s) 434, 830, 892
AflIII ACRYGT 1 cut(s) 142
AgeI ACCGGT 1 cut(s) 729
AgsI TTSAA 1 cut(s) 578
AhdI GACNNNNNGTC 1 cut(s) 66
AjiI CACGTC 1 cut(s) 143
AjnI CCWGG 1 cut(s) 645
AloI GAACNNNNNNTCC 2 cut(s) 771, 803
AluBI AGCT 3 cut(s) 121, 125, 667
AluI AGCT 3 cut(s) 121, 125, 667
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 470
AoxI GGCC 3 cut(s) 160, 675, 781
ApeKI GCWGC 2 cut(s) 118, 569
ApoI RAATTY 2 cut(s) 510, 845
AseI ATTAAT 1 cut(s) 363
AsiGI ACCGGT 1 cut(s) 729
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 160
AsuHPI GGTGA 2 cut(s) 171, 716
AsuNHI GCTAGC 1 cut(s) 121
AxyI CCTNAGG 1 cut(s) 720
BaeGI GKGCMC 2 cut(s) 560, 719
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 1 cut(s) 669
Bbv12I GWGCWC 1 cut(s) 669
BbvCI CCTCAGC 1 cut(s) 867
BbvI GCAGC 2 cut(s) 130, 581
BccI CCATC 2 cut(s) 470, 500
BceAI ACGGC 1 cut(s) 662
BciT130I CCWGG 1 cut(s) 647
BcoDI GTCTC 1 cut(s) 470
BfaI CTAG 1 cut(s) 122
BisI GCNGC 3 cut(s) 119, 163, 570
BlsI GCNGC 3 cut(s) 120, 164, 571
Bme1390I CCNGG 1 cut(s) 647
BmeRI GACNNNNNGTC 1 cut(s) 66
BmgBI CACGTC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 1 cut(s) 647
BmsI GCATC 3 cut(s) 73, 556, 891
BmtI GCTAGC 1 cut(s) 125
BpmI CTGGAG 1 cut(s) 807
Bpu10I CCTNAGC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 1 cut(s) 433
BsaWI WCCGGW 2 cut(s) 37, 729
BsaXI ACNNNNNCTCC 4 cut(s) 289, 319, 771, 801
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 830, 892
Bse118I RCCGGY 1 cut(s) 729
Bse1I ACTGG 1 cut(s) 175
Bse21I CCTNAGG 1 cut(s) 720
Bse3DI GCAATG 1 cut(s) 906
BseBI CCWGG 1 cut(s) 647
BseDI CCNNGG 1 cut(s) 433
BseGI GGATG 4 cut(s) 481, 511, 877, 882
BseLI CCNNNNNNNGG 3 cut(s) 434, 830, 892
BseMI GCAATG 1 cut(s) 906
BseMII CTCAG 2 cut(s) 711, 858
BseNI ACTGG 1 cut(s) 175
BseSI GKGCMC 2 cut(s) 560, 719
BseXI GCAGC 2 cut(s) 130, 581
Bsh1285I CGRYCG 2 cut(s) 42, 139
BshFI GGCC 3 cut(s) 162, 677, 783
BshNI GGYRCC 1 cut(s) 557
BshTI ACCGGT 1 cut(s) 729
BsiEI CGRYCG 2 cut(s) 42, 139
BsiHKAI GWGCWC 1 cut(s) 669
BsiSI CCGG 2 cut(s) 38, 730
BslFI GGGAC 1 cut(s) 786
BslI CCNNNNNNNGG 3 cut(s) 434, 830, 892
BsmAI GTCTC 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 786
BsnI GGCC 3 cut(s) 162, 677, 783
Bso31I GGTCTC 1 cut(s) 470
Bsp1286I GDGCHC 3 cut(s) 560, 669, 719
Bsp143I GATC 2 cut(s) 31, 43
BspACI CCGC 4 cut(s) 163, 438, 503, 604
BspANI GGCC 3 cut(s) 162, 677, 783
BspCNI CTCAG 2 cut(s) 712, 859
BspLI GGNNCC 1 cut(s) 559
BspOI GCTAGC 1 cut(s) 125
BspQI GCTCTTC 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 470
BsrBI CCGCTC 1 cut(s) 606
BsrDI GCAATG 1 cut(s) 906
BsrFI RCCGGY 1 cut(s) 729
BsrI ACTGG 1 cut(s) 175
BssAI RCCGGY 1 cut(s) 729
BssECI CCNNGG 1 cut(s) 433
BssMI GATC 2 cut(s) 31, 43
Bst2UI CCWGG 1 cut(s) 647
Bst4CI ACNGT 3 cut(s) 23, 153, 374
Bst6I CTCTTC 2 cut(s) 93, 234
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 2 cut(s) 720, 867
BstENI CCTNNNNNAGG 1 cut(s) 432
BstF5I GGATG 4 cut(s) 481, 511, 877, 882
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 2 cut(s) 34, 46
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 2 cut(s) 31, 43
BstMCI CGRYCG 2 cut(s) 42, 139
BstMWI GCNNNNNNNGC 2 cut(s) 105, 171
BstNI CCWGG 1 cut(s) 647
BstSCI CCNGG 1 cut(s) 645
BstSLI GKGCMC 2 cut(s) 560, 719
BstV1I GCAGC 2 cut(s) 130, 581
BstXI CCANNNNNNTGG 1 cut(s) 476
Bsu36I CCTNAGG 1 cut(s) 720
BsuRI GGCC 3 cut(s) 162, 677, 783
BtrI CACGTC 1 cut(s) 143
BtsCI GGATG 4 cut(s) 481, 511, 877, 882
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 3 cut(s) 19, 611, 834
Cac8I GCNNGC 1 cut(s) 123
CfoI GCGC 1 cut(s) 275
Cfr10I RCCGGY 1 cut(s) 729
Cfr13I GGNCC 1 cut(s) 160
CseI GACGC 1 cut(s) 88
CsiI ACCWGGT 1 cut(s) 645
Csp6I GTAC 2 cut(s) 635, 901
CspAI ACCGGT 1 cut(s) 729
CviAII CATG 4 cut(s) 80, 405, 807, 856
CviQI GTAC 2 cut(s) 635, 901
DdeI CTNAG 2 cut(s) 720, 867
DpnI GATC 2 cut(s) 33, 45
DpnII GATC 2 cut(s) 31, 43
DriI GACNNNNNGTC 1 cut(s) 66
EaeI YGGCCR 1 cut(s) 675
Eam1104I CTCTTC 2 cut(s) 93, 234
Eam1105I GACNNNNNGTC 1 cut(s) 66
EarI CTCTTC 2 cut(s) 93, 234
EciI GGCGGA 1 cut(s) 453
Ecl136II GAGCTC 1 cut(s) 667
Eco24I GRGCYC 1 cut(s) 669
Eco31I GGTCTC 1 cut(s) 470
Eco53kI GAGCTC 1 cut(s) 667
Eco81I CCTNAGG 1 cut(s) 720
EcoICRI GAGCTC 1 cut(s) 667
EcoNI CCTNNNNNAGG 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 645
EcoT38I GRGCYC 1 cut(s) 669
FaeI CATG 4 cut(s) 83, 408, 810, 859
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FaqI GGGAC 1 cut(s) 786
FatI CATG 4 cut(s) 79, 404, 806, 855
FauI CCCGC 1 cut(s) 611
FauNDI CATATG 1 cut(s) 104
Fnu4HI GCNGC 3 cut(s) 119, 163, 570
FokI GGATG 4 cut(s) 488, 518, 869, 884
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 3 cut(s) 119, 163, 570
FspBI CTAG 1 cut(s) 122
FspI TGCGCA 1 cut(s) 274
GlaI GCGC 1 cut(s) 274
GluI GCNGC 3 cut(s) 119, 163, 570
GsuI CTGGAG 1 cut(s) 807
HaeIII GGCC 3 cut(s) 162, 677, 783
HapII CCGG 2 cut(s) 38, 730
HgaI GACGC 1 cut(s) 88
HhaI GCGC 1 cut(s) 275
Hin1II CATG 4 cut(s) 83, 408, 810, 859
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HinfI GANTC 2 cut(s) 799, 820
HpaI GTTAAC 1 cut(s) 51
HpaII CCGG 2 cut(s) 38, 730
HphI GGTGA 2 cut(s) 171, 716
Hpy166II GTNNAC 3 cut(s) 51, 707, 727
Hpy188I TCNGA 3 cut(s) 31, 544, 792
Hpy188III TCNNGA 3 cut(s) 131, 317, 886
Hpy8I GTNNAC 3 cut(s) 51, 707, 727
Hpy99I CGWCG 3 cut(s) 137, 144, 544
HpyAV CCTTC 1 cut(s) 571
HpyCH4III ACNGT 3 cut(s) 23, 153, 374
HpyCH4IV ACGT 3 cut(s) 67, 142, 209
HpyCH4V TGCA 6 cut(s) 108, 157, 482, 569, 851, 882
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 171
HpyF3I CTNAG 2 cut(s) 720, 867
HpySE526I ACGT 3 cut(s) 67, 142, 209
Hsp92II CATG 4 cut(s) 83, 408, 810, 859
HspAI GCGC 1 cut(s) 273
KspAI GTTAAC 1 cut(s) 51
Kzo9I GATC 2 cut(s) 31, 43
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 2 cut(s) 611, 664
Lsp1109I GCAGC 2 cut(s) 130, 581
LweI GCATC 3 cut(s) 73, 556, 891
MabI ACCWGGT 1 cut(s) 645
MaeI CTAG 1 cut(s) 122
MaeII ACGT 3 cut(s) 67, 142, 209
MaeIII GTNAC 7 cut(s) 58, 167, 177, 238, 529, 744, 835
MalI GATC 2 cut(s) 33, 45
MbiI CCGCTC 1 cut(s) 606
MboI GATC 2 cut(s) 31, 43
MboII GAAGA 3 cut(s) 26, 80, 221
MhlI GDGCHC 3 cut(s) 560, 669, 719
MluCI AATT 9 cut(s) 248, 349, 360, 441, 510, 546, 754, 772, 845
MlyI GAGTC 2 cut(s) 808, 814
MmeI TCCRAC 2 cut(s) 332, 522
MnlI CCTC 8 cut(s) 237, 270, 428, 460, 715, 773, 814, 862
MseI TTAA 5 cut(s) 50, 363, 651, 768, 913
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 165
MspI CCGG 2 cut(s) 38, 730
MspR9I CCNGG 1 cut(s) 647
MvaI CCWGG 1 cut(s) 647
MwoI GCNNNNNNNGC 2 cut(s) 105, 171
NdeI CATATG 1 cut(s) 104
NdeII GATC 2 cut(s) 31, 43
NheI GCTAGC 1 cut(s) 121
NlaIII CATG 4 cut(s) 83, 408, 810, 859
NlaIV GGNNCC 1 cut(s) 559
NmeAIII GCCGAG 1 cut(s) 809
NmuCI GTSAC 4 cut(s) 58, 177, 238, 744
NsbI TGCGCA 1 cut(s) 274
PciSI GCTCTTC 1 cut(s) 93
PinAI ACCGGT 1 cut(s) 729
PkrI GCNGC 3 cut(s) 120, 164, 571
PleI GAGTC 2 cut(s) 807, 814
PpsI GAGTC 2 cut(s) 807, 814
PshBI ATTAAT 1 cut(s) 363
Psp124BI GAGCTC 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 645
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 1 cut(s) 160
RsaI GTAC 2 cut(s) 636, 902
RsaNI GTAC 2 cut(s) 635, 901
RseI CAYNNNNRTG 1 cut(s) 474
SacI GAGCTC 1 cut(s) 669
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 5 cut(s) 50, 363, 651, 768, 913
SatI GCNGC 3 cut(s) 119, 163, 570
Sau3AI GATC 2 cut(s) 31, 43
Sau96I GGNCC 1 cut(s) 160
SchI GAGTC 2 cut(s) 808, 814
ScrFI CCNGG 1 cut(s) 647
SduI GDGCHC 3 cut(s) 560, 669, 719
SexAI ACCWGGT 1 cut(s) 645
SfaNI GCATC 3 cut(s) 73, 556, 891
SmiMI CAYNNNNRTG 1 cut(s) 474
Sse9I AATT 9 cut(s) 248, 349, 360, 441, 510, 546, 754, 772, 845
SsiI CCGC 4 cut(s) 163, 438, 503, 604
SspI AATATT 2 cut(s) 367, 421
SspMI CTAG 1 cut(s) 122
SstI GAGCTC 1 cut(s) 669
StyD4I CCNGG 1 cut(s) 645
TaaI ACNGT 3 cut(s) 23, 153, 374
TaiI ACGT 3 cut(s) 70, 145, 212
TaqI TCGA 4 cut(s) 42, 564, 812, 818
TaqII GACCGA 1 cut(s) 153
TasI AATT 9 cut(s) 248, 349, 360, 441, 510, 546, 754, 772, 845
TatI WGTACW 1 cut(s) 900
TauI GCSGC 1 cut(s) 165
Tru1I TTAA 5 cut(s) 50, 363, 651, 768, 913
Tru9I TTAA 5 cut(s) 50, 363, 651, 768, 913
TscAI CASTG 3 cut(s) 26, 618, 834
TseFI GTSAC 4 cut(s) 58, 177, 238, 744
TseI GCWGC 2 cut(s) 118, 569
Tsp45I GTSAC 4 cut(s) 58, 177, 238, 744
TspDTI ATGAA 1 cut(s) 209
TspRI CASTG 3 cut(s) 26, 618, 834
VspI ATTAAT 1 cut(s) 363
XagI CCTNNNNNAGG 1 cut(s) 432
XapI RAATTY 2 cut(s) 510, 845
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.