FvH4_4g20671

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000673
Physical Location & Seq
Forward (+)
23811722 .. 23812846
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20671.t1

Sequence Viewer

Length: 723 bp
ATGTCAAACTCATGGTCTGATCTTGTTCCAGGTGATATCTTCTCTCTAATTGCTGAACATCTTTCGTTTAAGGACCTAGTTCGCCTTACTCTGATATGCAAGACTTGGCGACCGGTCGTTCTTGAAGCTATTCAACACCATCCCCTTATGTGTTTGCCATGGCTAACTAATTCGCATGATCCTTGGAATAGTACGATGCGCATTGGACCAATCTTCAACAATATGTCGACCAAACAAACTTACCACTCTCTTAAAGAAACGGTTGTGAGTAAAGAGTCTGTATTGATGGTGTTTTACGTACATTGCCTTTATTATAATGAGATTGATGGTTCGTTCATGATCAAATTGCTTACCTTCAATGTCACATATGAATGTTGGAGTACTGTGAAGAACAACTACAGAATAGCTACCACCAACACCGCAGTTAAAGAATGGAGACTAAAAGAGCGAGAGTACAAACGTGACGGCAAGTTTTATAGCTTGGTTAAGTTGACTGATGGGGGAGCTGTTATTAGTGATCAGGAATTTCGAGATGATAGGTGTAAGCAGTGGTCAGCATTGGCAGTTCCAACAGTAGGAGAGGCTAGGAAATTTGCAAACATGATAGTTGTTGTTGAAGGGAAGCGATGCCTGTGTTTTACCTGCAACACTGAAGGCCATTGGATATATCAAGAAACTTACTTACCCTATGGACAAGGACAAAGGATACTACAGTTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

241

Amino Acids

27.96

Weight (kDa)

8.07

Isoelectric Point (pI)

29.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 315
Acc16I TGCGCA 1 cut(s) 200
Acc36I ACCTGC 1 cut(s) 650
AccI GTMKAC 1 cut(s) 227
AciI CCGC 1 cut(s) 420
AclWI GGATC 1 cut(s) 173
AcsI RAATTY 2 cut(s) 524, 590
AcuI CTGAAG 1 cut(s) 672
AfaI GTAC 4 cut(s) 193, 300, 382, 455
AfiI CCNNNNNNNGG 1 cut(s) 575
AgeI ACCGGT 1 cut(s) 112
AgsI TTSAA 5 cut(s) 125, 134, 217, 358, 617
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 4 cut(s) 128, 407, 480, 506
AluI AGCT 4 cut(s) 128, 407, 480, 506
Alw26I GTCTC 1 cut(s) 430
AlwI GGATC 1 cut(s) 173
AoxI GGCC 1 cut(s) 655
ApoI RAATTY 2 cut(s) 524, 590
ArsI GACNNNNNNTTYG 1 cut(s) 31
AsiGI ACCGGT 1 cut(s) 112
Asp700I GAANNNNTTC 1 cut(s) 129
AspLEI GCGC 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 73, 206
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 2 cut(s) 73, 206
BaeI ACNNNNGTAYC 1 cut(s) 698
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
BccI CCATC 4 cut(s) 147, 280, 320, 491
BceAI ACGGC 1 cut(s) 481
BciT130I CCWGG 1 cut(s) 30
BciVI GTATCC 1 cut(s) 699
BclI TGATCA 2 cut(s) 339, 517
BcoDI GTCTC 1 cut(s) 430
BfaI CTAG 2 cut(s) 77, 585
BfmI CTRYAG 2 cut(s) 397, 710
BfuAI ACCTGC 1 cut(s) 650
BfuI GTATCC 1 cut(s) 699
BmcAI AGTACT 1 cut(s) 382
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 2 cut(s) 73, 206
BmgT120I GGNCC 2 cut(s) 73, 206
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 2 cut(s) 186, 617
BsaAI YACGTR 1 cut(s) 298
BsaJI CCNNGG 2 cut(s) 158, 182
BsaWI WCCGGW 1 cut(s) 112
Bsc4I CCNNNNNNNGG 1 cut(s) 575
Bse118I RCCGGY 1 cut(s) 112
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 158, 182
BseGI GGATG 1 cut(s) 139
BseLI CCNNNNNNNGG 1 cut(s) 575
BseMI GCAATG 1 cut(s) 301
Bsh1285I CGRYCG 2 cut(s) 113, 117
BshFI GGCC 1 cut(s) 657
BshTI ACCGGT 1 cut(s) 112
BsiEI CGRYCG 2 cut(s) 113, 117
BsiSI CCGG 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 575
BsmAI GTCTC 1 cut(s) 430
BsnI GGCC 1 cut(s) 657
Bsp143I GATC 4 cut(s) 19, 178, 339, 517
Bsp19I CCATGG 1 cut(s) 158
BspACI CCGC 1 cut(s) 420
BspANI GGCC 1 cut(s) 657
BspHI TCATGA 1 cut(s) 336
BspMI ACCTGC 1 cut(s) 650
BspPI GGATC 1 cut(s) 173
BsrDI GCAATG 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 112
BssECI CCNNGG 2 cut(s) 158, 182
BssMI GATC 4 cut(s) 19, 178, 339, 517
BssT1I CCWWGG 2 cut(s) 158, 182
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 4 cut(s) 262, 385, 574, 714
BstBAI YACGTR 1 cut(s) 298
BstDSI CCRYGG 1 cut(s) 158
BstF5I GGATG 1 cut(s) 139
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 4 cut(s) 22, 181, 342, 520
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 4 cut(s) 19, 178, 339, 517
BstMCI CGRYCG 2 cut(s) 113, 117
BstNI CCWGG 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 2 cut(s) 397, 710
BstSNI TACGTA 1 cut(s) 298
BsuI GTATCC 1 cut(s) 699
BsuRI GGCC 1 cut(s) 657
BtgI CCRYGG 1 cut(s) 158
BtgZI GCGATG 1 cut(s) 640
BtsCI GGATG 1 cut(s) 139
BtsI GCAGTG 1 cut(s) 554
BtsIMutI CAGTG 2 cut(s) 554, 648
BveI ACCTGC 1 cut(s) 650
CciI TCATGA 1 cut(s) 336
CfoI GCGC 1 cut(s) 201
Cfr10I RCCGGY 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 73, 206
Csp6I GTAC 4 cut(s) 192, 299, 381, 454
CspAI ACCGGT 1 cut(s) 112
CviAII CATG 5 cut(s) 12, 159, 176, 337, 601
CviJI RGCY 7 cut(s) 128, 163, 407, 480, 506, 584, 657
CviKI_1 RGCY 7 cut(s) 128, 163, 407, 480, 506, 584, 657
CviQI GTAC 4 cut(s) 192, 299, 381, 454
DpnI GATC 4 cut(s) 21, 180, 341, 519
DpnII GATC 4 cut(s) 19, 178, 339, 517
Eco105I TACGTA 1 cut(s) 298
Eco130I CCWWGG 2 cut(s) 158, 182
Eco32I GATATC 1 cut(s) 37
Eco47I GGWCC 2 cut(s) 73, 206
Eco57I CTGAAG 1 cut(s) 672
EcoO109I RGGNCCY 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 28
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 2 cut(s) 158, 182
ErhI CCWWGG 2 cut(s) 158, 182
FaeI CATG 5 cut(s) 15, 162, 179, 340, 604
FatI CATG 5 cut(s) 11, 158, 175, 336, 600
FauNDI CATATG 1 cut(s) 367
FbaI TGATCA 2 cut(s) 339, 517
FblI GTMKAC 1 cut(s) 227
FokI GGATG 1 cut(s) 126
FspAI RTGCGCAY 1 cut(s) 200
FspBI CTAG 2 cut(s) 77, 585
FspI TGCGCA 1 cut(s) 200
GlaI GCGC 1 cut(s) 200
HaeIII GGCC 1 cut(s) 657
HapII CCGG 1 cut(s) 113
HhaI GCGC 1 cut(s) 201
Hin1II CATG 5 cut(s) 15, 162, 179, 340, 604
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HincII GTYRAC 2 cut(s) 228, 492
HindII GTYRAC 2 cut(s) 228, 492
HinfI GANTC 1 cut(s) 275
HpaII CCGG 1 cut(s) 113
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 2 cut(s) 228, 492
Hpy188I TCNGA 2 cut(s) 19, 93
Hpy188III TCNNGA 5 cut(s) 122, 337, 521, 530, 671
Hpy8I GTNNAC 2 cut(s) 228, 492
HpyAV CCTTC 3 cut(s) 364, 611, 647
HpyCH4III ACNGT 4 cut(s) 262, 385, 574, 714
HpyCH4IV ACGT 2 cut(s) 297, 460
HpyCH4V TGCA 3 cut(s) 99, 596, 645
HpySE526I ACGT 2 cut(s) 297, 460
Hsp92II CATG 5 cut(s) 15, 162, 179, 340, 604
HspAI GCGC 1 cut(s) 199
Ksp22I TGATCA 2 cut(s) 339, 517
Kzo9I GATC 4 cut(s) 19, 178, 339, 517
LmnI GCTCC 1 cut(s) 503
LpnPI CCDG 6 cut(s) 15, 42, 126, 506, 644, 655
LweI GCATC 2 cut(s) 186, 617
MaeI CTAG 2 cut(s) 77, 585
MaeII ACGT 2 cut(s) 297, 460
MaeIII GTNAC 2 cut(s) 361, 461
MalI GATC 4 cut(s) 21, 180, 341, 519
MboI GATC 4 cut(s) 19, 178, 339, 517
MboII GAAGA 3 cut(s) 31, 205, 400
MluCI AATT 5 cut(s) 48, 169, 344, 524, 590
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 3 cut(s) 356, 593, 696
MnlI CCTC 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 129
MseI TTAA 4 cut(s) 69, 252, 426, 486
MslI CAYNNNNRTG 1 cut(s) 370
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
NcoI CCATGG 1 cut(s) 158
NdeI CATATG 1 cut(s) 367
NdeII GATC 4 cut(s) 19, 178, 339, 517
NlaIII CATG 5 cut(s) 15, 162, 179, 340, 604
NmuCI GTSAC 2 cut(s) 361, 461
NsbI TGCGCA 1 cut(s) 200
PagI TCATGA 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 129
PinAI ACCGGT 1 cut(s) 112
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
Ppu21I YACGTR 1 cut(s) 298
PpuMI RGGWCCY 1 cut(s) 73
PsiI TTATAA 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspPI GGNCC 2 cut(s) 73, 206
PspPPI RGGWCCY 1 cut(s) 73
RsaI GTAC 4 cut(s) 193, 300, 382, 455
RsaNI GTAC 4 cut(s) 192, 299, 381, 454
RseI CAYNNNNRTG 1 cut(s) 370
SalI GTCGAC 1 cut(s) 226
SaqAI TTAA 4 cut(s) 69, 252, 426, 486
Sau3AI GATC 4 cut(s) 19, 178, 339, 517
Sau96I GGNCC 2 cut(s) 73, 206
ScaI AGTACT 1 cut(s) 382
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 1 cut(s) 30
SfaNI GCATC 2 cut(s) 186, 617
SfcI CTRYAG 2 cut(s) 397, 710
SinI GGWCC 2 cut(s) 73, 206
SmiMI CAYNNNNRTG 1 cut(s) 370
SnaBI TACGTA 1 cut(s) 298
Sse9I AATT 5 cut(s) 48, 169, 344, 524, 590
SsiI CCGC 1 cut(s) 420
SspMI CTAG 2 cut(s) 77, 585
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 2 cut(s) 158, 182
TaaI ACNGT 4 cut(s) 262, 385, 574, 714
TaiI ACGT 2 cut(s) 300, 463
TaqI TCGA 2 cut(s) 227, 529
TasI AATT 5 cut(s) 48, 169, 344, 524, 590
TatI WGTACW 2 cut(s) 380, 453
Tru1I TTAA 4 cut(s) 69, 252, 426, 486
Tru9I TTAA 4 cut(s) 69, 252, 426, 486
TscAI CASTG 2 cut(s) 554, 655
TseFI GTSAC 2 cut(s) 361, 461
Tsp45I GTSAC 2 cut(s) 361, 461
TspDTI ATGAA 2 cut(s) 325, 384
TspRI CASTG 2 cut(s) 554, 655
VpaK11BI GGWCC 2 cut(s) 73, 206
XapI RAATTY 2 cut(s) 524, 590
XmiI GTMKAC 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 129
XspI CTAG 2 cut(s) 77, 585
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.