FvH4_2g26662

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000673
Physical Location & Seq
Forward (+)
21376494 .. 21377935
1442 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26662.t1

Sequence Viewer

Length: 1224 bp
ATGACAACGATGATTTCTGAATGGGTTCGATCTGGTGTGGGAAAATCTCAAGCTCTACTAGTGAAGCTGTTATTGCTCGCTCCCAATTTCTCCTCTCGCCTCCAGCCGAGGACGACATCTCAAGAAGAAGAAGAAGAAGAATGGCGATGTTTCAATGGTAGTAATAATAATAACTCGATGCATATTGCAGAGAATAAACATTCTAGTTGGTCCGACCTTCCGCTCGATATCTTATGTGAAGTGGCCAATCGTCTCCTATACTGGGATCTTGTTCGTTCTGCTGCCGTATGCAAGACCTGGTGGCGTGTCATATATGGGGCTCATCTTCCACAACGCTCCACATTGCTTGTGCCCTACCTGATGAGCTATACCTGTAATCGTGATGGTAAACACATAGAGTTTTTGGACATGCAGGGAGAAACTTACAAATATACAGTCAAGCACGAACTCTTGGTCGACGGAACCAATCCAGGACCCTATGCGTCAAAGTATGGTTGGTTGCTTCTGAATAGATATGGGTTGAACAAAGAACAGTTCTATCTATACAATCCCTTCTGCAATGAGGTTATACAACTACCCGAGTTGGATTTGGAGCCGGGATTTTATGTAGAGATTGCAACATTCACAGCCCCTTCGACTTCTCCCAACTGTGTTATCTTTGTCCTCTGCAGAAAGTATCAGAGTAGAAGTCAAAACTGCATCATAAGCACTTATAAGCTCGGAAATAGAAGATGGAGTAATTATAAAACTACATGTCAGGGTTCTGTCGAGGGAGTTACTTACGTAGATGGAATTTTCTATTGTATTTTTCCAGAGAAAAATCACTTAGATGGTTCGTCGTCGGTGTTTATCAGTACCTTCGATATAGCAGTTCAAAAATGGGTCATGGACTATACATCCATAAAAATAGAAGACCATAATGAAAGCAGAACCTTTTACTTGGTTGAGTCGATAAATGGGAAGCTATTACTGGCAGTGTCTGACCTAGCTCATGATGTTAGAAGCCACTGCTGGTTTCTATACCAATTTGATTGGTTACGAAAAGATTGGACTATGGTTCGAAGTTTGGGGCACCAAGTATTGCTTCTAAGTAGCTCATCGTCTATTTCTAGTCCCACATTGGGGAAGGATAATAAATTATCAGACATGATATTTCATCTTAACTGGGGAAATGAGCCTTTTACTTGGAGCAGCGACGGCGTTTGGAGAAAAGCTCTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

408

Amino Acids

47.04

Weight (kDa)

6.42

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 159 - 373 1e-24 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 714, 744
AccB1I GGYRCC 1 cut(s) 1071
AccBSI CCGCTC 1 cut(s) 223
AccI GTMKAC 1 cut(s) 456
AciI CCGC 1 cut(s) 221
AclWI GGATC 1 cut(s) 273
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 792
AfaI GTAC 1 cut(s) 856
AfiI CCNNNNNNNGG 3 cut(s) 262, 1121, 1122
AflIII ACRYGT 1 cut(s) 752
AgsI TTSAA 3 cut(s) 154, 523, 875
AhlI ACTAGT 1 cut(s) 58
AjnI CCWGG 2 cut(s) 296, 469
AjuI GAANNNNNNNTTGG 2 cut(s) 1068, 1100
AluBI AGCT 8 cut(s) 53, 67, 366, 718, 964, 989, 1095, 1214
AluI AGCT 8 cut(s) 53, 67, 366, 718, 964, 989, 1095, 1214
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 1 cut(s) 273
AlwNI CAGNNNCTG 1 cut(s) 980
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 1 cut(s) 243
ApeKI GCWGC 2 cut(s) 281, 1191
ApoI RAATTY 1 cut(s) 792
ArsI GACNNNNNNTTYG 4 cut(s) 420, 438, 452, 470
Asp700I GAANNNNTTC 1 cut(s) 24
AspS9I GGNCC 2 cut(s) 210, 473
AsuC2I CCSGG 1 cut(s) 597
AsuII TTCGAA 1 cut(s) 1060
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 2 cut(s) 210, 473
BaeGI GKGCMC 2 cut(s) 354, 1074
BalI TGGCCA 1 cut(s) 245
BanI GGYRCC 1 cut(s) 1071
BanII GRGCYC 1 cut(s) 322
BbsI GAAGAC 1 cut(s) 918
BbvI GCAGC 2 cut(s) 268, 1203
BccI CCATC 4 cut(s) 377, 726, 782, 824
BceAI ACGGC 2 cut(s) 269, 1213
BciT130I CCWGG 2 cut(s) 298, 471
BcnI CCSGG 1 cut(s) 597
BcoDI GTCTC 1 cut(s) 257
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 4 cut(s) 59, 204, 986, 1110
BfmI CTRYAG 1 cut(s) 667
BisI GCNGC 2 cut(s) 282, 1192
BlsI GCNGC 2 cut(s) 283, 1193
Bme1390I CCNGG 3 cut(s) 298, 471, 597
Bme18I GGWCC 2 cut(s) 210, 473
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 2 cut(s) 210, 473
BmiI GGNNCC 4 cut(s) 463, 475, 594, 1073
BmrFI CCNGG 3 cut(s) 298, 471, 597
BmrI ACTGGG 2 cut(s) 271, 1174
BmsI GCATC 2 cut(s) 168, 708
BmuI ACTGGG 2 cut(s) 271, 1174
BpiI GAAGAC 1 cut(s) 918
BplI GAGNNNNNCTC 1 cut(s) 1198
BpmI CTGGAG 1 cut(s) 86
Bpu14I TTCGAA 1 cut(s) 1060
BpuEI CTTGAG 2 cut(s) 33, 105
BpuMI CCSGG 1 cut(s) 597
BsaAI YACGTR 1 cut(s) 784
BsaJI CCNNGG 1 cut(s) 107
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 1121, 1122
Bse1I ACTGG 3 cut(s) 266, 975, 1169
Bse3DI GCAATG 2 cut(s) 341, 565
BseBI CCWGG 2 cut(s) 298, 471
BseDI CCNNGG 1 cut(s) 107
BseGI GGATG 1 cut(s) 896
BseLI CCNNNNNNNGG 3 cut(s) 262, 1121, 1122
BseMI GCAATG 2 cut(s) 341, 565
BseNI ACTGG 3 cut(s) 266, 975, 1169
BseRI GAGGAG 1 cut(s) 82
BseSI GKGCMC 2 cut(s) 354, 1074
BseXI GCAGC 2 cut(s) 268, 1203
BshFI GGCC 1 cut(s) 245
BshNI GGYRCC 1 cut(s) 1071
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 1 cut(s) 596
BslFI GGGAC 1 cut(s) 1098
BslI CCNNNNNNNGG 3 cut(s) 262, 1121, 1122
BsmAI GTCTC 1 cut(s) 257
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 1098
BsnI GGCC 1 cut(s) 245
BsoBI CYCGRG 1 cut(s) 578
Bsp119I TTCGAA 1 cut(s) 1060
Bsp1286I GDGCHC 3 cut(s) 322, 354, 1074
Bsp143I GATC 2 cut(s) 29, 265
BspACI CCGC 1 cut(s) 221
BspANI GGCC 1 cut(s) 245
BspHI TCATGA 1 cut(s) 991
BspLI GGNNCC 4 cut(s) 463, 475, 594, 1073
BspMAI CTGCAG 1 cut(s) 671
BspPI GGATC 1 cut(s) 273
BspT104I TTCGAA 1 cut(s) 1060
BspT107I GGYRCC 1 cut(s) 1071
BsrBI CCGCTC 1 cut(s) 223
BsrDI GCAATG 2 cut(s) 341, 565
BsrI ACTGG 3 cut(s) 266, 975, 1169
BssECI CCNNGG 1 cut(s) 107
BssMI GATC 2 cut(s) 29, 265
Bst2UI CCWGG 2 cut(s) 298, 471
Bst4CI ACNGT 3 cut(s) 436, 534, 650
BstBAI YACGTR 1 cut(s) 784
BstBI TTCGAA 1 cut(s) 1060
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 2 cut(s) 826, 1088
BstF5I GGATG 1 cut(s) 896
BstKTI GATC 2 cut(s) 32, 268
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 2 cut(s) 29, 265
BstMWI GCNNNNNNNGC 3 cut(s) 73, 705, 1197
BstNI CCWGG 2 cut(s) 298, 471
BstNSI RCATGY 2 cut(s) 412, 756
BstSCI CCNGG 3 cut(s) 296, 469, 595
BstSFI CTRYAG 1 cut(s) 667
BstSLI GKGCMC 2 cut(s) 354, 1074
BstSNI TACGTA 1 cut(s) 784
BstV1I GCAGC 2 cut(s) 268, 1203
BstV2I GAAGAC 1 cut(s) 918
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 1 cut(s) 245
BtgZI GCGATG 1 cut(s) 160
BtsCI GGATG 1 cut(s) 896
BtsI GCAGTG 2 cut(s) 981, 1006
BtsIMutI CAGTG 2 cut(s) 981, 1006
Cac8I GCNNGC 1 cut(s) 78
CaiI CAGNNNCTG 1 cut(s) 980
CciI TCATGA 1 cut(s) 991
Cfr13I GGNCC 2 cut(s) 210, 473
CseI GACGC 1 cut(s) 471
CsiI ACCWGGT 1 cut(s) 296
Csp6I GTAC 1 cut(s) 855
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 5 cut(s) 409, 753, 886, 992, 1147
CviQI GTAC 1 cut(s) 855
DdeI CTNAG 2 cut(s) 826, 1088
DpnI GATC 2 cut(s) 31, 267
DpnII GATC 2 cut(s) 29, 265
EaeI YGGCCR 1 cut(s) 243
Eco105I TACGTA 1 cut(s) 784
Eco24I GRGCYC 1 cut(s) 322
Eco32I GATATC 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 210, 473
Eco88I CYCGRG 1 cut(s) 578
EcoO109I RGGNCCY 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 296, 469
EcoRV GATATC 1 cut(s) 229
EcoT22I ATGCAT 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 322
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 5 cut(s) 412, 756, 889, 995, 1150
FalI AAGNNNNNCTT 3 cut(s) 1068, 1100, 1203
FaqI GGGAC 1 cut(s) 1098
FatI CATG 5 cut(s) 408, 752, 885, 991, 1146
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 2 cut(s) 282, 1192
FokI GGATG 1 cut(s) 883
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 2 cut(s) 282, 1192
FspBI CTAG 4 cut(s) 59, 204, 986, 1110
GluI GCNGC 2 cut(s) 282, 1192
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 1 cut(s) 245
HapII CCGG 1 cut(s) 596
HgaI GACGC 1 cut(s) 471
Hin1II CATG 5 cut(s) 412, 756, 889, 995, 1150
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HinfI GANTC 1 cut(s) 947
HpaII CCGG 1 cut(s) 596
Hpy166II GTNNAC 2 cut(s) 389, 457
Hpy188I TCNGA 7 cut(s) 19, 214, 507, 681, 722, 982, 1144
Hpy188III TCNNGA 4 cut(s) 122, 380, 812, 992
Hpy8I GTNNAC 2 cut(s) 389, 457
Hpy99I CGWCG 4 cut(s) 461, 841, 844, 1199
HpyAV CCTTC 5 cut(s) 227, 562, 642, 868, 1120
HpyCH4III ACNGT 3 cut(s) 436, 534, 650
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 8 cut(s) 181, 188, 291, 412, 558, 617, 669, 699
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 705, 1197
HpyF3I CTNAG 2 cut(s) 826, 1088
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 5 cut(s) 412, 756, 889, 995, 1150
Kzo9I GATC 2 cut(s) 29, 265
LmnI GCTCC 4 cut(s) 85, 341, 592, 1188
Lsp1109I GCAGC 2 cut(s) 268, 1203
LweI GCATC 2 cut(s) 168, 708
MabI ACCWGGT 1 cut(s) 296
MaeI CTAG 4 cut(s) 59, 204, 986, 1110
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 2 cut(s) 775, 1035
MalI GATC 2 cut(s) 31, 267
MbiI CCGCTC 1 cut(s) 223
MboI GATC 2 cut(s) 29, 265
MboII GAAGA 8 cut(s) 137, 140, 143, 146, 149, 317, 741, 923
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 3 cut(s) 322, 354, 1074
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 5 cut(s) 85, 739, 792, 1025, 1136
MluNI TGGCCA 1 cut(s) 245
MlyI GAGTC 1 cut(s) 956
MmeI TCCRAC 2 cut(s) 237, 564
MnlI CCTC 6 cut(s) 102, 103, 110, 556, 674, 763
Mox20I TGGCCA 1 cut(s) 245
Mph1103I ATGCAT 1 cut(s) 183
MroXI GAANNNNTTC 1 cut(s) 24
MscI TGGCCA 1 cut(s) 245
MseI TTAA 2 cut(s) 1161, 1222
MslI CAYNNNNRTG 1 cut(s) 828
Msp20I TGGCCA 1 cut(s) 245
MspI CCGG 1 cut(s) 596
MspR9I CCNGG 3 cut(s) 298, 471, 597
MvaI CCWGG 2 cut(s) 298, 471
MwoI GCNNNNNNNGC 3 cut(s) 73, 705, 1197
NciI CCSGG 1 cut(s) 597
NdeII GATC 2 cut(s) 29, 265
NlaIII CATG 5 cut(s) 412, 756, 889, 995, 1150
NlaIV GGNNCC 4 cut(s) 463, 475, 594, 1073
NmeAIII GCCGAG 1 cut(s) 132
NsiI ATGCAT 1 cut(s) 183
NspI RCATGY 2 cut(s) 412, 756
NspV TTCGAA 1 cut(s) 1060
PagI TCATGA 1 cut(s) 991
PciI ACATGT 1 cut(s) 752
PdmI GAANNNNTTC 1 cut(s) 24
PfoI TCCNGGA 1 cut(s) 469
PkrI GCNGC 2 cut(s) 283, 1193
PleI GAGTC 1 cut(s) 955
PpsI GAGTC 1 cut(s) 955
Ppu21I YACGTR 1 cut(s) 784
PpuMI RGGWCCY 1 cut(s) 473
PscI ACATGT 1 cut(s) 752
PsiI TTATAA 2 cut(s) 714, 744
Psp5II RGGWCCY 1 cut(s) 473
Psp6I CCWGG 2 cut(s) 296, 469
PspGI CCWGG 2 cut(s) 296, 469
PspN4I GGNNCC 4 cut(s) 463, 475, 594, 1073
PspPI GGNCC 2 cut(s) 210, 473
PspPPI RGGWCCY 1 cut(s) 473
PstI CTGCAG 1 cut(s) 671
PstNI CAGNNNCTG 1 cut(s) 980
PsuI RGATCY 1 cut(s) 265
RsaI GTAC 1 cut(s) 856
RsaNI GTAC 1 cut(s) 855
RseI CAYNNNNRTG 1 cut(s) 828
SalI GTCGAC 1 cut(s) 455
SaqAI TTAA 2 cut(s) 1161, 1222
SatI GCNGC 2 cut(s) 282, 1192
Sau3AI GATC 2 cut(s) 29, 265
Sau96I GGNCC 2 cut(s) 210, 473
SchI GAGTC 1 cut(s) 956
ScrFI CCNGG 3 cut(s) 298, 471, 597
SduI GDGCHC 3 cut(s) 322, 354, 1074
SexAI ACCWGGT 1 cut(s) 296
SfaNI GCATC 2 cut(s) 168, 708
SfcI CTRYAG 1 cut(s) 667
SfuI TTCGAA 1 cut(s) 1060
SinI GGWCC 2 cut(s) 210, 473
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 2 cut(s) 48, 120
SmoI CTYRAG 2 cut(s) 48, 120
SnaBI TACGTA 1 cut(s) 784
SpeI ACTAGT 1 cut(s) 58
Sse9I AATT 5 cut(s) 85, 739, 792, 1025, 1136
SsiI CCGC 1 cut(s) 221
SspMI CTAG 4 cut(s) 59, 204, 986, 1110
StyD4I CCNGG 3 cut(s) 296, 469, 595
TaaI ACNGT 3 cut(s) 436, 534, 650
TaiI ACGT 1 cut(s) 786
TaqI TCGA 9 cut(s) 28, 176, 225, 456, 635, 768, 861, 950, 1060
TasI AATT 5 cut(s) 85, 739, 792, 1025, 1136
Tru1I TTAA 2 cut(s) 1161, 1222
Tru9I TTAA 2 cut(s) 1161, 1222
TscAI CASTG 2 cut(s) 981, 1013
TseI GCWGC 2 cut(s) 281, 1191
TspDTI ATGAA 2 cut(s) 936, 1145
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 2 cut(s) 981, 1013
VpaK11BI GGWCC 2 cut(s) 210, 473
XapI RAATTY 1 cut(s) 792
XceI RCATGY 2 cut(s) 412, 756
XmiI GTMKAC 1 cut(s) 456
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 4 cut(s) 59, 204, 986, 1110
Zsp2I ATGCAT 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.