RLG00000011844

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000673
Physical Location & Seq
Forward (+)
13227779 .. 13229214
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011844

Sequence Viewer

Length: 1197 bp
ATGGTAGACAATAATCTTTCTTCATGGTCAGATCCTCCAATCGATATCTTATGCGCGGTAGTTATTCGTCTCCTCTCCGTATGCGAGTATAAAGATCTGGTTCGTCTTTCTGCTGTATGCAGGAGCTGGAGATTTATTATATATAGGGTCTATCTTCAACCAATCGATATTTTATGCGAGGTCGCTAATCGTCTCCAATATGAAGAAATCGTTCGTCTTTCTTCTGTATGCAAGATCTGGAGATTTATTATATATAGGGTCTATCTTCAACTACGCTCCCCTCTCCGTATGCCAGGCCTGAGTTTATACAGTCCATATCATCAAGACGAACACGGCCGGGTAATAAGCTTCTTTGATATATCAATCCAGAAAATCCGCCGTATAAAGCTGAAGATTCGAGAAGAATTAAAAACATTCTGGCAACTTGATTCAAAATATGGTTGGATCATTTTGCAAAATGGGTGCAAATCCAATCCTGCACTCGTCTTGTATAATCCTTTAACCAAAGAGATTATACAACTGCCACAGTTGAACCTAAGCACCAATAGTGAATGTATAGCAACATTCACAAGCCCTCCGACTTCTCCTGACTGTGTTATCTTTGCCTTCTACCAAGTAGAAAGAGGAGAAGCTTGCATTGTAGGCACATGTAAGCTGCGAGATAATATATGGAGATTTCGTAAAATAACTGACTGTAAGGGTTGGAAGGGCACGAACCGAGTTACGAATGTGACCTATTTCAATGGAATTTTCTGTTTTGTTATTAAAAAAAGGTTATCTGATTGGTCGGTAGAGTTGGGTACCTTGGATGTATCGGTTCAAGACTGGGTCATGGACTATGCTTCCATAACAGTAGCACAAGAACAGTATCGGTATTGGAAGTGGTATTGGCTGGTTGAGTCTGATGGGAAGCTGTTATTGGCAGTTTATCGTGGCCATGGGGATGTTACAATGAGCTATTGGCATGTTTACCAATTCGATTGGTCACGAAAGGATTGGACTAGGGTTCGAGGTTTAGGAAACCAGGTATTGATTTTACGTCGACATGATTGGTCCAGGTTGACATCATTTGCTTGTCCCACATCCGGAGACGATAGTAAACTTTCAAACATGATATTTTGTGATGGCGTTTGGGGACGCGGTGCCCTTTTTTATACTGGCAGCAGTGATGGCGTTTGGAGAATCGCCAGCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

399

Amino Acids

46.62

Weight (kDa)

9.03

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 54 - 87 2.7e-06 F-box domain
Beta-prop_KIB1-4 PF03478 117 - 351 1.5e-21 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 800
AccB1I GGYRCC 2 cut(s) 800, 1142
AccI GTMKAC 2 cut(s) 6, 1042
AccII CGCG 2 cut(s) 56, 1140
AccIII TCCGGA 1 cut(s) 1085
AciI CCGC 3 cut(s) 56, 376, 1140
AclWI GGATC 2 cut(s) 26, 452
AcoI YGGCCR 2 cut(s) 334, 934
AcsI RAATTY 1 cut(s) 747
AcuI CTGAAG 1 cut(s) 410
AfaI GTAC 1 cut(s) 802
AfiI CCNNNNNNNGG 1 cut(s) 1085
AflIII ACRYGT 1 cut(s) 647
AgsI TTSAA 7 cut(s) 158, 269, 432, 532, 742, 821, 1107
AjnI CCWGG 3 cut(s) 292, 1023, 1055
AjuI GAANNNNNNNTTGG 2 cut(s) 902, 934
AluBI AGCT 7 cut(s) 126, 348, 388, 632, 655, 913, 957
AluI AGCT 7 cut(s) 126, 348, 388, 632, 655, 913, 957
Alw26I GTCTC 3 cut(s) 74, 197, 1083
AlwI GGATC 2 cut(s) 26, 452
AlwNI CAGNNNCTG 1 cut(s) 126
Aor13HI TCCGGA 1 cut(s) 1085
AoxI GGCC 3 cut(s) 295, 334, 934
ApeKI GCWGC 2 cut(s) 655, 1161
ApoI RAATTY 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 210
Asp718I GGTACC 1 cut(s) 800
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 1053
AsuC2I CCSGG 1 cut(s) 338
AvaII GGWCC 1 cut(s) 1053
BaeGI GKGCMC 2 cut(s) 713, 1147
BalI TGGCCA 1 cut(s) 936
BanI GGYRCC 2 cut(s) 800, 1142
BbvI GCAGC 2 cut(s) 642, 1173
BccI CCATC 3 cut(s) 899, 1118, 1163
BceAI ACGGC 2 cut(s) 349, 363
BciT130I CCWGG 3 cut(s) 294, 1025, 1057
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 3 cut(s) 74, 197, 1083
BfaI CTAG 1 cut(s) 1002
BglII AGATCT 2 cut(s) 94, 234
BisI GCNGC 2 cut(s) 656, 1162
BlsI GCNGC 2 cut(s) 657, 1163
Bme1390I CCNGG 4 cut(s) 294, 338, 1025, 1057
Bme18I GGWCC 1 cut(s) 1053
BmgT120I GGNCC 1 cut(s) 1053
BmiI GGNNCC 2 cut(s) 802, 1144
BmrFI CCNGG 4 cut(s) 294, 338, 1025, 1057
BmrI ACTGGG 1 cut(s) 835
BmuI ACTGGG 1 cut(s) 835
BpmI CTGGAG 2 cut(s) 148, 259
Bpu10I CCTNAGC 1 cut(s) 536
BpuMI CCSGG 1 cut(s) 338
Bsa29I ATCGAT 2 cut(s) 42, 165
BsaJI CCNNGG 2 cut(s) 804, 937
BsaWI WCCGGW 1 cut(s) 1085
BsaXI ACNNNNNCTCC 4 cut(s) 559, 589, 664, 694
Bsc4I CCNNNNNNNGG 1 cut(s) 1085
Bse1I ACTGG 2 cut(s) 830, 1162
BseAI TCCGGA 1 cut(s) 1085
BseBI CCWGG 3 cut(s) 294, 1025, 1057
BseCI ATCGAT 2 cut(s) 42, 165
BseDI CCNNGG 2 cut(s) 804, 937
BseGI GGATG 3 cut(s) 814, 949, 1082
BseLI CCNNNNNNNGG 1 cut(s) 1085
BseMII CTCAG 1 cut(s) 290
BseNI ACTGG 2 cut(s) 830, 1162
BseRI GAGGAG 2 cut(s) 62, 639
BseSI GKGCMC 2 cut(s) 713, 1147
BseX3I CGGCCG 1 cut(s) 334
BseXI GCAGC 2 cut(s) 642, 1173
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 2 cut(s) 56, 1140
Bsh1285I CGRYCG 1 cut(s) 337
BshFI GGCC 3 cut(s) 297, 336, 936
BshNI GGYRCC 2 cut(s) 800, 1142
BshVI ATCGAT 2 cut(s) 42, 165
BsiEI CGRYCG 1 cut(s) 337
BsiSI CCGG 2 cut(s) 337, 1086
BslFI GGGAC 2 cut(s) 1062, 1149
BslI CCNNNNNNNGG 1 cut(s) 1085
BsmAI GTCTC 3 cut(s) 74, 197, 1083
BsmBI CGTCTC 3 cut(s) 74, 197, 1083
BsmFI GGGAC 2 cut(s) 1062, 1149
BsnI GGCC 3 cut(s) 297, 336, 936
Bsp1286I GDGCHC 2 cut(s) 713, 1147
Bsp13I TCCGGA 1 cut(s) 1085
Bsp143I GATC 4 cut(s) 31, 94, 234, 444
Bsp19I CCATGG 1 cut(s) 937
BspACI CCGC 3 cut(s) 56, 376, 1140
BspANI GGCC 3 cut(s) 297, 336, 936
BspCNI CTCAG 1 cut(s) 291
BspDI ATCGAT 2 cut(s) 42, 165
BspEI TCCGGA 1 cut(s) 1085
BspFNI CGCG 2 cut(s) 56, 1140
BspLI GGNNCC 2 cut(s) 802, 1144
BspPI GGATC 2 cut(s) 26, 452
BspT107I GGYRCC 2 cut(s) 800, 1142
BsrI ACTGG 2 cut(s) 830, 1162
BssECI CCNNGG 2 cut(s) 804, 937
BssMI GATC 4 cut(s) 31, 94, 234, 444
BssT1I CCWWGG 2 cut(s) 804, 937
Bst2UI CCWGG 3 cut(s) 294, 1025, 1057
Bst4CI ACNGT 6 cut(s) 311, 528, 593, 695, 853, 867
BstC8I GCNNGC 2 cut(s) 634, 1189
BstDEI CTNAG 2 cut(s) 299, 536
BstDSI CCRYGG 1 cut(s) 937
BstF5I GGATG 3 cut(s) 814, 949, 1082
BstFNI CGCG 2 cut(s) 56, 1140
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 4 cut(s) 34, 97, 237, 447
BstMAI GTCTC 3 cut(s) 74, 197, 1083
BstMBI GATC 4 cut(s) 31, 94, 234, 444
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 2 cut(s) 642, 1170
BstNI CCWGG 3 cut(s) 294, 1025, 1057
BstNSI RCATGY 2 cut(s) 651, 968
BstSCI CCNGG 4 cut(s) 292, 336, 1023, 1055
BstSLI GKGCMC 2 cut(s) 713, 1147
BstUI CGCG 2 cut(s) 56, 1140
BstV1I GCAGC 2 cut(s) 642, 1173
BstX2I RGATCY 3 cut(s) 31, 94, 234
BstYI RGATCY 3 cut(s) 31, 94, 234
BstZI CGGCCG 1 cut(s) 334
Bsu15I ATCGAT 2 cut(s) 42, 165
BsuRI GGCC 3 cut(s) 297, 336, 936
BsuTUI ATCGAT 2 cut(s) 42, 165
BtgI CCRYGG 1 cut(s) 937
BtsCI GGATG 3 cut(s) 814, 949, 1082
BtsI GCAGTG 1 cut(s) 1171
BtsIMutI CAGTG 1 cut(s) 1171
Cac8I GCNNGC 2 cut(s) 634, 1189
CaiI CAGNNNCTG 1 cut(s) 126
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 1053
ClaI ATCGAT 2 cut(s) 42, 165
CseI GACGC 1 cut(s) 1146
CsiI ACCWGGT 1 cut(s) 1023
Csp6I GTAC 1 cut(s) 801
CviAII CATG 7 cut(s) 24, 648, 832, 938, 965, 1046, 1111
CviQI GTAC 1 cut(s) 801
DdeI CTNAG 2 cut(s) 299, 536
DpnI GATC 4 cut(s) 33, 96, 236, 446
DpnII GATC 4 cut(s) 31, 94, 234, 444
EaeI YGGCCR 2 cut(s) 334, 934
EagI CGGCCG 1 cut(s) 334
EciI GGCGGA 1 cut(s) 365
EclXI CGGCCG 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 804, 937
Eco147I AGGCCT 1 cut(s) 297
Eco32I GATATC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 1053
Eco52I CGGCCG 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 410
EcoRII CCWGG 3 cut(s) 292, 1023, 1055
EcoRV GATATC 1 cut(s) 46
EcoT14I CCWWGG 2 cut(s) 804, 937
ErhI CCWWGG 2 cut(s) 804, 937
Esp3I CGTCTC 3 cut(s) 74, 197, 1083
FaeI CATG 7 cut(s) 27, 651, 835, 941, 968, 1049, 1114
FaqI GGGAC 2 cut(s) 1062, 1149
FatI CATG 7 cut(s) 23, 647, 831, 937, 964, 1045, 1110
FblI GTMKAC 2 cut(s) 6, 1042
Fnu4HI GCNGC 2 cut(s) 656, 1162
FokI GGATG 3 cut(s) 821, 956, 1069
Fsp4HI GCNGC 2 cut(s) 656, 1162
FspBI CTAG 1 cut(s) 1002
GlaI GCGC 1 cut(s) 55
GluI GCNGC 2 cut(s) 656, 1162
GsuI CTGGAG 2 cut(s) 148, 259
HaeIII GGCC 3 cut(s) 297, 336, 936
HapII CCGG 2 cut(s) 337, 1086
HgaI GACGC 1 cut(s) 1146
HhaI GCGC 1 cut(s) 56
Hin1II CATG 7 cut(s) 27, 651, 835, 941, 968, 1049, 1114
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 2 cut(s) 1043, 1062
HindII GTYRAC 2 cut(s) 1043, 1062
HindIII AAGCTT 2 cut(s) 346, 630
HinfI GANTC 4 cut(s) 394, 428, 899, 1182
HpaII CCGG 2 cut(s) 337, 1086
Hpy166II GTNNAC 5 cut(s) 7, 970, 1043, 1062, 1100
Hpy188I TCNGA 4 cut(s) 31, 579, 781, 904
Hpy188III TCNNGA 8 cut(s) 238, 323, 367, 398, 587, 821, 987, 1086
Hpy8I GTNNAC 5 cut(s) 7, 970, 1043, 1062, 1100
Hpy99I CGWCG 1 cut(s) 1044
HpyAV CCTTC 2 cut(s) 616, 700
HpyCH4III ACNGT 6 cut(s) 311, 528, 593, 695, 853, 867
HpyCH4IV ACGT 1 cut(s) 1039
HpyCH4V TGCA 6 cut(s) 120, 231, 454, 465, 479, 636
HpyF10VI GCNNNNNNNGC 2 cut(s) 642, 1170
HpyF3I CTNAG 2 cut(s) 299, 536
HpySE526I ACGT 1 cut(s) 1039
Hsp92II CATG 7 cut(s) 27, 651, 835, 941, 968, 1049, 1114
HspAI GCGC 1 cut(s) 54
Kpn2I TCCGGA 1 cut(s) 1085
KpnI GGTACC 1 cut(s) 804
Kzo9I GATC 4 cut(s) 31, 94, 234, 444
LmnI GCTCC 2 cut(s) 123, 281
Lsp1109I GCAGC 2 cut(s) 642, 1173
MabI ACCWGGT 1 cut(s) 1023
MaeI CTAG 1 cut(s) 1002
MaeII ACGT 1 cut(s) 1039
MaeIII GTNAC 4 cut(s) 721, 730, 946, 984
MalI GATC 4 cut(s) 33, 96, 236, 446
MboI GATC 4 cut(s) 31, 94, 234, 444
MboII GAAGA 7 cut(s) 12, 146, 213, 215, 257, 403, 413
MflI RGATCY 3 cut(s) 31, 94, 234
MhlI GDGCHC 2 cut(s) 713, 1147
MlsI TGGCCA 1 cut(s) 936
MluCI AATT 3 cut(s) 404, 747, 974
MluNI TGGCCA 1 cut(s) 936
MlyI GAGTC 1 cut(s) 908
MmeI TCCRAC 3 cut(s) 422, 602, 683
MnlI CCTC 7 cut(s) 45, 83, 172, 291, 585, 617, 1004
Mox20I TGGCCA 1 cut(s) 936
MroI TCCGGA 1 cut(s) 1085
MroXI GAANNNNTTC 1 cut(s) 210
MscI TGGCCA 1 cut(s) 936
MseI TTAA 3 cut(s) 407, 500, 765
MslI CAYNNNNRTG 1 cut(s) 942
Msp20I TGGCCA 1 cut(s) 936
MspI CCGG 2 cut(s) 337, 1086
MspR9I CCNGG 4 cut(s) 294, 338, 1025, 1057
MvaI CCWGG 3 cut(s) 294, 1025, 1057
MvnI CGCG 2 cut(s) 56, 1140
MwoI GCNNNNNNNGC 2 cut(s) 642, 1170
NciI CCSGG 1 cut(s) 338
NcoI CCATGG 1 cut(s) 937
NdeII GATC 4 cut(s) 31, 94, 234, 444
NlaIII CATG 7 cut(s) 27, 651, 835, 941, 968, 1049, 1114
NlaIV GGNNCC 2 cut(s) 802, 1144
NmuCI GTSAC 2 cut(s) 730, 984
NspI RCATGY 2 cut(s) 651, 968
PceI AGGCCT 1 cut(s) 297
PciI ACATGT 1 cut(s) 647
PdmI GAANNNNTTC 1 cut(s) 210
PfeI GAWTC 3 cut(s) 394, 428, 1182
PflFI GACNNNGTC 1 cut(s) 827
PkrI GCNGC 2 cut(s) 657, 1163
PleI GAGTC 1 cut(s) 907
PpsI GAGTC 1 cut(s) 907
PscI ACATGT 1 cut(s) 647
Psp6I CCWGG 3 cut(s) 292, 1023, 1055
PspGI CCWGG 3 cut(s) 292, 1023, 1055
PspN4I GGNNCC 2 cut(s) 802, 1144
PspPI GGNCC 1 cut(s) 1053
PstNI CAGNNNCTG 1 cut(s) 126
PsuI RGATCY 3 cut(s) 31, 94, 234
PsyI GACNNNGTC 1 cut(s) 827
RsaI GTAC 1 cut(s) 802
RsaNI GTAC 1 cut(s) 801
RseI CAYNNNNRTG 1 cut(s) 942
SalI GTCGAC 1 cut(s) 1041
SaqAI TTAA 3 cut(s) 407, 500, 765
SatI GCNGC 2 cut(s) 656, 1162
Sau3AI GATC 4 cut(s) 31, 94, 234, 444
Sau96I GGNCC 1 cut(s) 1053
SchI GAGTC 1 cut(s) 908
ScrFI CCNGG 4 cut(s) 294, 338, 1025, 1057
SduI GDGCHC 2 cut(s) 713, 1147
SexAI ACCWGGT 1 cut(s) 1023
SinI GGWCC 1 cut(s) 1053
SmiMI CAYNNNNRTG 1 cut(s) 942
Sse9I AATT 3 cut(s) 404, 747, 974
SseBI AGGCCT 1 cut(s) 297
SsiI CCGC 3 cut(s) 56, 376, 1140
SspMI CTAG 1 cut(s) 1002
StuI AGGCCT 1 cut(s) 297
StyD4I CCNGG 4 cut(s) 292, 336, 1023, 1055
StyI CCWWGG 2 cut(s) 804, 937
TaaI ACNGT 6 cut(s) 311, 528, 593, 695, 853, 867
TaiI ACGT 1 cut(s) 1042
TaqI TCGA 6 cut(s) 42, 165, 397, 978, 1009, 1042
TasI AATT 3 cut(s) 404, 747, 974
TfiI GAWTC 3 cut(s) 394, 428, 1182
Tru1I TTAA 3 cut(s) 407, 500, 765
Tru9I TTAA 3 cut(s) 407, 500, 765
TscAI CASTG 1 cut(s) 1171
TseFI GTSAC 2 cut(s) 730, 984
TseI GCWGC 2 cut(s) 655, 1161
Tsp45I GTSAC 2 cut(s) 730, 984
TspDTI ATGAA 2 cut(s) 12, 216
TspGWI ACGGA 2 cut(s) 67, 275
TspRI CASTG 1 cut(s) 1171
Tth111I GACNNNGTC 1 cut(s) 827
VpaK11BI GGWCC 1 cut(s) 1053
XapI RAATTY 1 cut(s) 747
XceI RCATGY 2 cut(s) 651, 968
XmiI GTMKAC 2 cut(s) 6, 1042
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 1 cut(s) 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.