FvH4_4g17781

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000673
Physical Location & Seq
Reverse (-)
21760648 .. 21762094
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17781.t1

Sequence Viewer

Length: 1290 bp
ATGGCTAGGATGGATCAGAATGGTGAGTACATTCCTAGAACTGATAAAGATGCGCAGAACTGCAGCAACAAATTAGGCTCTGCAGGTGTCACTGGAGGTAGCAATACTAGTACTAGGTTATATAGAGGTAGAGGTACTTGCTACAAGTGCGGTGAGTCTGGGCACTTCTGGCGGGAATGCACTACAAGAACACAAGAAAAAGACCTGCAGCTTTACAATAGTTCTTCATCATGGTCAGATATTTCAACTGATATCTTTAGCCTAGTCACTGAGCGTCTCCTTGTTGATGATGTTATTCGCTTGGGGGCTGTATGCAAGAGTTGGCAGCAAGCTATTCGAGTTCAACCTCGTTGTCATGCAAGTATGCCTTGGGTAATGAAGACTTCGGCTTTCTCTTGCAACTTCAAGGTTTCTGACTTTTGGAGAAATCGAACTTACTATCTTGATAGAATAGTAAACAAAGATTTCGAAGAATTATGTCATAATCATCATGGAAAATTCAATATTTGTGATTCAAAATATGGTTGGTTGTTGCTCTTCTTCAAAGAACACGAGTACTCCATCACAAGGCAGCATAAAGAGTTTGAAGTCCATTTCTTGTTATACAATCCTTTGGTTAATGAGAAAATCAAATTGCCTCCATTACATTTGCTAGACGATGAACATGGAGAGATAGCAAAGTTCATGACAACTCCATCTGCAGGCCGTCCACCTACTTGGGATATATTTTTCATGTGTTATCAGAACCATGGTGGAGGAACAAAAGATTACATGTTGTATACATGTAATGAAGATAGACGCTGGAGTAGGCATAAAATTGATGTGAATGCATTTGATTGGTACGTAAAGTGTGACGTTGCTTATGCAGATGAAACTTTATACTGTGCTTTTCGTTCTCCTGGATCAGTAGTGATGGGTAGCTACAACGTTGCACTTCAACATTTTGCCATGGATCCTTCCCTTTCCTTCAACATATTTGGTTTTGATAACCTGTACCTTGCTGAGTTTGATGGAGATCTGTATTGCATTTCTATTAAACCTCGTGTTTGTTGTCATGTTTACCAGCTTGATCGGCCACGAAAAGTATGGAGTCTAGTTAAAAGCTTGGGAAATCGGGTTTTGATTCTGTCCGGCCGCTCGTCATGTATTGTAGTTCCAGCCGTGGGAGAAGCTGAGGAATATGCAGGCATGTTATTTTTATATGGGAACGACGACCTACAGGCCTTTATCAGCGACACCAAAATTTCACCAACGGCATCTTTTACTAGATTATTACTTCAGAGAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

430

Amino Acids

49.47

Weight (kDa)

7.17

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 167 - 384 1.2e-15 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 74
Acc16I TGCGCA 1 cut(s) 54
Acc36I ACCTGC 2 cut(s) 74, 213
AccBSI CCGCTC 1 cut(s) 1137
AccI GTMKAC 1 cut(s) 779
AciI CCGC 3 cut(s) 150, 172, 1135
AclI AACGTT 1 cut(s) 927
AclWI GGATC 4 cut(s) 21, 910, 947, 960
AcoI YGGCCR 2 cut(s) 1073, 1132
AcsI RAATTY 2 cut(s) 497, 1242
AcuI CTGAAG 1 cut(s) 1262
AfaI GTAC 6 cut(s) 29, 112, 136, 557, 842, 995
AfiI CCNNNNNNNGG 3 cut(s) 567, 701, 1163
AflIII ACRYGT 2 cut(s) 771, 782
AgsI TTSAA 9 cut(s) 246, 344, 406, 502, 516, 544, 587, 938, 970
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 1 cut(s) 898
AluBI AGCT 6 cut(s) 211, 332, 921, 1066, 1104, 1172
AluI AGCT 6 cut(s) 211, 332, 921, 1066, 1104, 1172
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 4 cut(s) 21, 910, 947, 960
AoxI GGCC 4 cut(s) 703, 1073, 1132, 1221
ApeKI GCWGC 4 cut(s) 63, 208, 325, 571
ApoI RAATTY 2 cut(s) 497, 1242
AspLEI GCGC 1 cut(s) 55
AsuHPI GGTGA 3 cut(s) 35, 164, 1239
AsuII TTCGAA 1 cut(s) 468
BaeGI GKGCMC 1 cut(s) 165
BamHI GGATCC 1 cut(s) 952
BauI CACGAG 2 cut(s) 551, 1041
BbsI GAAGAC 1 cut(s) 386
BbvCI CCTCAGC 1 cut(s) 1173
BbvI GCAGC 4 cut(s) 75, 220, 337, 583
BccI CCATC 5 cut(s) 4, 569, 703, 907, 1004
BceAI ACGGC 3 cut(s) 690, 1145, 1269
BcgI CGANNNNNNTGC 2 cut(s) 317, 351
BciT130I CCWGG 1 cut(s) 900
BcoDI GTCTC 1 cut(s) 281
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 8 cut(s) 6, 36, 108, 114, 263, 653, 1094, 1266
BfmI CTRYAG 5 cut(s) 61, 81, 206, 699, 1217
BfuAI ACCTGC 2 cut(s) 74, 213
BglII AGATCT 1 cut(s) 1015
BisI GCNGC 5 cut(s) 64, 209, 326, 572, 1135
BlsI GCNGC 5 cut(s) 65, 210, 327, 573, 1136
BmcAI AGTACT 2 cut(s) 112, 557
Bme1390I CCNGG 1 cut(s) 900
BmiI GGNNCC 1 cut(s) 954
BmrFI CCNGG 1 cut(s) 900
BmsI GCATC 2 cut(s) 40, 1265
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 2 cut(s) 114, 823
Bpu10I CCTNAGC 1 cut(s) 1173
Bpu14I TTCGAA 1 cut(s) 468
BsaAI YACGTR 1 cut(s) 844
BsaBI GATNNNNATC 1 cut(s) 1014
BsaJI CCNNGG 4 cut(s) 368, 748, 948, 1161
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 3 cut(s) 567, 701, 1163
Bse1I ACTGG 1 cut(s) 97
Bse8I GATNNNNATC 1 cut(s) 1014
BseBI CCWGG 1 cut(s) 900
BseDI CCNNGG 4 cut(s) 368, 748, 948, 1161
BseGI GGATG 1 cut(s) 15
BseJI GATNNNNATC 1 cut(s) 1014
BseLI CCNNNNNNNGG 3 cut(s) 567, 701, 1163
BseMII CTCAG 3 cut(s) 261, 993, 1164
BseNI ACTGG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 165
BseX3I CGGCCG 1 cut(s) 1132
BseXI GCAGC 4 cut(s) 75, 220, 337, 583
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 4 cut(s) 705, 1075, 1134, 1223
BsiEI CGRYCG 1 cut(s) 1135
BsiSI CCGG 1 cut(s) 1131
BslI CCNNNNNNNGG 3 cut(s) 567, 701, 1163
BsmAI GTCTC 1 cut(s) 281
BsmBI CGTCTC 1 cut(s) 281
BsmI GAATGC 2 cut(s) 182, 832
BsnI GGCC 4 cut(s) 705, 1075, 1134, 1223
Bsp119I TTCGAA 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 5 cut(s) 13, 902, 952, 1015, 1069
Bsp19I CCATGG 2 cut(s) 748, 948
BspACI CCGC 3 cut(s) 150, 172, 1135
BspANI GGCC 4 cut(s) 705, 1075, 1134, 1223
BspCNI CTCAG 3 cut(s) 262, 994, 1165
BspHI TCATGA 1 cut(s) 684
BspLI GGNNCC 1 cut(s) 954
BspMAI CTGCAG 4 cut(s) 65, 85, 210, 703
BspMI ACCTGC 2 cut(s) 74, 213
BspPI GGATC 4 cut(s) 21, 910, 947, 960
BspQI GCTCTTC 1 cut(s) 542
BspT104I TTCGAA 1 cut(s) 468
BsrBI CCGCTC 1 cut(s) 1137
BsrI ACTGG 1 cut(s) 97
BssECI CCNNGG 4 cut(s) 368, 748, 948, 1161
BssMI GATC 5 cut(s) 13, 902, 952, 1015, 1069
BssNAI GTATAC 1 cut(s) 780
BssSI CACGAG 2 cut(s) 551, 1041
BssT1I CCWWGG 3 cut(s) 368, 748, 948
Bst1107I GTATAC 1 cut(s) 780
Bst2BI CACGAG 2 cut(s) 551, 1041
Bst2UI CCWGG 1 cut(s) 900
Bst4CI ACNGT 1 cut(s) 884
Bst6I CTCTTC 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 844
BstBI TTCGAA 1 cut(s) 468
BstC8I GCNNGC 3 cut(s) 330, 703, 1186
BstDEI CTNAG 3 cut(s) 270, 1002, 1173
BstDSI CCRYGG 3 cut(s) 748, 948, 1161
BstF5I GGATG 1 cut(s) 15
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 5 cut(s) 16, 905, 955, 1018, 1072
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 5 cut(s) 13, 902, 952, 1015, 1069
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 3 cut(s) 147, 169, 1072
BstNI CCWGG 1 cut(s) 900
BstNSI RCATGY 3 cut(s) 775, 786, 1192
BstSCI CCNGG 1 cut(s) 898
BstSFI CTRYAG 5 cut(s) 61, 81, 206, 699, 1217
BstSLI GKGCMC 1 cut(s) 165
BstSNI TACGTA 1 cut(s) 844
BstV1I GCAGC 4 cut(s) 75, 220, 337, 583
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 2 cut(s) 952, 1015
BstXI CCANNNNNNTGG 1 cut(s) 717
BstYI RGATCY 2 cut(s) 952, 1015
BstZ17I GTATAC 1 cut(s) 780
BstZI CGGCCG 1 cut(s) 1132
BsuRI GGCC 4 cut(s) 705, 1075, 1134, 1223
BtgI CCRYGG 3 cut(s) 748, 948, 1161
BtsCI GGATG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 90, 267
BveI ACCTGC 2 cut(s) 74, 213
Cac8I GCNNGC 3 cut(s) 330, 703, 1186
CciI TCATGA 1 cut(s) 684
CfoI GCGC 1 cut(s) 55
CseI GACGC 2 cut(s) 263, 807
Csp6I GTAC 6 cut(s) 28, 111, 135, 556, 841, 994
CviQI GTAC 6 cut(s) 28, 111, 135, 556, 841, 994
DdeI CTNAG 3 cut(s) 270, 1002, 1173
DpnI GATC 5 cut(s) 15, 904, 954, 1017, 1071
DpnII GATC 5 cut(s) 13, 902, 952, 1015, 1069
EaeI YGGCCR 2 cut(s) 1073, 1132
EagI CGGCCG 1 cut(s) 1132
Eam1104I CTCTTC 1 cut(s) 542
EarI CTCTTC 1 cut(s) 542
EclXI CGGCCG 1 cut(s) 1132
Eco105I TACGTA 1 cut(s) 844
Eco130I CCWWGG 3 cut(s) 368, 748, 948
Eco147I AGGCCT 1 cut(s) 1223
Eco32I GATATC 1 cut(s) 253
Eco52I CGGCCG 1 cut(s) 1132
Eco57I CTGAAG 1 cut(s) 1262
EcoRII CCWGG 1 cut(s) 898
EcoRV GATATC 1 cut(s) 253
EcoT14I CCWWGG 3 cut(s) 368, 748, 948
EcoT22I ATGCAT 1 cut(s) 832
ErhI CCWWGG 3 cut(s) 368, 748, 948
Esp3I CGTCTC 1 cut(s) 281
FalI AAGNNNNNCTT 2 cut(s) 352, 384
FauI CCCGC 1 cut(s) 165
FblI GTMKAC 1 cut(s) 779
Fnu4HI GCNGC 5 cut(s) 64, 209, 326, 572, 1135
FokI GGATG 1 cut(s) 22
Fsp4HI GCNGC 5 cut(s) 64, 209, 326, 572, 1135
FspBI CTAG 8 cut(s) 6, 36, 108, 114, 263, 653, 1094, 1266
FspI TGCGCA 1 cut(s) 54
GlaI GCGC 1 cut(s) 54
GluI GCNGC 5 cut(s) 64, 209, 326, 572, 1135
GsuI CTGGAG 2 cut(s) 114, 823
HaeIII GGCC 4 cut(s) 705, 1075, 1134, 1223
HapII CCGG 1 cut(s) 1131
HgaI GACGC 2 cut(s) 263, 807
HhaI GCGC 1 cut(s) 55
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HindIII AAGCTT 1 cut(s) 1102
HinfI GANTC 4 cut(s) 155, 512, 1090, 1123
HpaII CCGG 1 cut(s) 1131
HphI GGTGA 3 cut(s) 35, 164, 1239
Hpy166II GTNNAC 4 cut(s) 457, 710, 780, 1060
Hpy188I TCNGA 5 cut(s) 18, 238, 415, 744, 1281
Hpy188III TCNNGA 2 cut(s) 443, 685
Hpy8I GTNNAC 4 cut(s) 457, 710, 780, 1060
Hpy99I CGWCG 1 cut(s) 1214
HpyAV CCTTC 2 cut(s) 966, 976
HpyCH4III ACNGT 1 cut(s) 884
HpyCH4IV ACGT 3 cut(s) 843, 855, 927
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 169, 1072
HpyF3I CTNAG 3 cut(s) 270, 1002, 1173
HpySE526I ACGT 3 cut(s) 843, 855, 927
HspAI GCGC 1 cut(s) 53
Kzo9I GATC 5 cut(s) 13, 902, 952, 1015, 1069
LguI GCTCTTC 1 cut(s) 542
Lsp1109I GCAGC 4 cut(s) 75, 220, 337, 583
LweI GCATC 2 cut(s) 40, 1265
MaeI CTAG 8 cut(s) 6, 36, 108, 114, 263, 653, 1094, 1266
MaeII ACGT 3 cut(s) 843, 855, 927
MaeIII GTNAC 3 cut(s) 88, 265, 851
MalI GATC 5 cut(s) 15, 904, 954, 1017, 1071
MbiI CCGCTC 1 cut(s) 1137
MboI GATC 5 cut(s) 13, 902, 952, 1015, 1069
MboII GAAGA 6 cut(s) 216, 391, 482, 529, 532, 803
MflI RGATCY 2 cut(s) 952, 1015
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 6 cut(s) 71, 473, 497, 632, 816, 1242
MlyI GAGTC 2 cut(s) 164, 1099
MnlI CCTC 8 cut(s) 89, 119, 125, 357, 648, 749, 1050, 1168
Mph1103I ATGCAT 1 cut(s) 832
MseI TTAA 3 cut(s) 618, 1035, 1098
MspI CCGG 1 cut(s) 1131
MspR9I CCNGG 1 cut(s) 900
Mva1269I GAATGC 2 cut(s) 182, 832
MvaI CCWGG 1 cut(s) 900
MwoI GCNNNNNNNGC 3 cut(s) 147, 169, 1072
NcoI CCATGG 2 cut(s) 748, 948
NdeII GATC 5 cut(s) 13, 902, 952, 1015, 1069
NlaIV GGNNCC 1 cut(s) 954
NmuCI GTSAC 3 cut(s) 88, 265, 851
NsbI TGCGCA 1 cut(s) 54
NsiI ATGCAT 1 cut(s) 832
NspI RCATGY 3 cut(s) 775, 786, 1192
NspV TTCGAA 1 cut(s) 468
PagI TCATGA 1 cut(s) 684
PaqCI CACCTGC 1 cut(s) 74
PceI AGGCCT 1 cut(s) 1223
PciI ACATGT 2 cut(s) 771, 782
PciSI GCTCTTC 1 cut(s) 542
PctI GAATGC 2 cut(s) 182, 832
PfeI GAWTC 2 cut(s) 512, 1123
PfoI TCCNGGA 1 cut(s) 898
PkrI GCNGC 5 cut(s) 65, 210, 327, 573, 1136
PleI GAGTC 2 cut(s) 163, 1098
PpsI GAGTC 2 cut(s) 163, 1098
Ppu21I YACGTR 1 cut(s) 844
PscI ACATGT 2 cut(s) 771, 782
Psp1406I AACGTT 1 cut(s) 927
Psp6I CCWGG 1 cut(s) 898
PspGI CCWGG 1 cut(s) 898
PspN4I GGNNCC 1 cut(s) 954
PstI CTGCAG 4 cut(s) 65, 85, 210, 703
PsuI RGATCY 2 cut(s) 952, 1015
RsaI GTAC 6 cut(s) 29, 112, 136, 557, 842, 995
RsaNI GTAC 6 cut(s) 28, 111, 135, 556, 841, 994
SapI GCTCTTC 1 cut(s) 542
SaqAI TTAA 3 cut(s) 618, 1035, 1098
SatI GCNGC 5 cut(s) 64, 209, 326, 572, 1135
Sau3AI GATC 5 cut(s) 13, 902, 952, 1015, 1069
ScaI AGTACT 2 cut(s) 112, 557
SchI GAGTC 2 cut(s) 164, 1099
ScrFI CCNGG 1 cut(s) 900
SduI GDGCHC 1 cut(s) 165
SfaNI GCATC 2 cut(s) 40, 1265
SfcI CTRYAG 5 cut(s) 61, 81, 206, 699, 1217
SfuI TTCGAA 1 cut(s) 468
SnaBI TACGTA 1 cut(s) 844
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 6 cut(s) 71, 473, 497, 632, 816, 1242
SseBI AGGCCT 1 cut(s) 1223
SsiI CCGC 3 cut(s) 150, 172, 1135
SspI AATATT 1 cut(s) 505
SspMI CTAG 8 cut(s) 6, 36, 108, 114, 263, 653, 1094, 1266
StuI AGGCCT 1 cut(s) 1223
StyD4I CCNGG 1 cut(s) 898
StyI CCWWGG 3 cut(s) 368, 748, 948
TaaI ACNGT 1 cut(s) 884
TaiI ACGT 3 cut(s) 846, 858, 930
TaqI TCGA 3 cut(s) 337, 430, 468
TasI AATT 6 cut(s) 71, 473, 497, 632, 816, 1242
TatI WGTACW 3 cut(s) 27, 110, 555
TauI GCSGC 1 cut(s) 1137
TfiI GAWTC 2 cut(s) 512, 1123
Tru1I TTAA 3 cut(s) 618, 1035, 1098
Tru9I TTAA 3 cut(s) 618, 1035, 1098
TscAI CASTG 2 cut(s) 97, 274
TseFI GTSAC 3 cut(s) 88, 265, 851
TseI GCWGC 4 cut(s) 63, 208, 325, 571
Tsp45I GTSAC 3 cut(s) 88, 265, 851
TspDTI ATGAA 7 cut(s) 216, 392, 673, 675, 721, 804, 885
TspRI CASTG 2 cut(s) 97, 274
XapI RAATTY 2 cut(s) 497, 1242
XceI RCATGY 3 cut(s) 775, 786, 1192
XcmI CCANNNNNNNNNTGG 1 cut(s) 1083
XmiI GTMKAC 1 cut(s) 779
XspI CTAG 8 cut(s) 6, 36, 108, 114, 263, 653, 1094, 1266
ZrmI AGTACT 2 cut(s) 112, 557
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.