FvH4_2g27260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000655
Physical Location & Seq
Reverse (-)
21680215 .. 21681002
788 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27260.t1

Sequence Viewer

Length: 537 bp
ATGCATGGCATACCGAGGCACAGCAGCCGTACCCAAAAGATAGTACCAGATCCAAGTAAAACAGAAGCCCAAATATATTATATATATAAATCACCATATATAGCTATCTTCTCAAAAACAGAAACCCTAGATCCATCGAGATTGCGGCTATCGGATCGAATTGATATGGCTGGTGGTGCCCGTTGCCGTGTCCGTTCAGGCGGAGGGTTGAGATCGTCCGGAAGAAAATCCACCGGATCAGTAAGTATCAGTGAACATCAAGAGAGCCTTGTGAGGAGATATCCTGGTGCAACTTATATTGTTCTTCGTCCAAGATCAGGATCTGCCGTCAGCGCTTCGCTTCCTGACGATTATCAGTTTGATATGTGCCAGAATATTCTGGATCAGTACTATGATATGAGCCGCTTGTGTGGGAGCGACTTTCCTTTGTGTGAAACACGTCGCGGCACTATACTCGACCTTTGTGGTATCGAGGTGCCTCCTGATGATTTAGACAAGAAGGTCTCTACTTCCACCAATGCAGCAGCTCAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.71

Weight (kDa)

8.83

Isoelectric Point (pI)

56.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 500
AccB1I GGYRCC 2 cut(s) 176, 475
AccII CGCG 1 cut(s) 444
AccIII TCCGGA 1 cut(s) 218
AciI CCGC 4 cut(s) 145, 201, 403, 444
AclWI GGATC 6 cut(s) 44, 125, 162, 244, 328, 390
AfaI GTAC 3 cut(s) 31, 45, 389
AfeI AGCGCT 1 cut(s) 334
AfiI CCNNNNNNNGG 1 cut(s) 317
AflIII ACRYGT 1 cut(s) 437
AjiI CACGTC 1 cut(s) 440
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 2 cut(s) 104, 527
AluI AGCT 2 cut(s) 104, 527
Alw26I GTCTC 1 cut(s) 508
AlwI GGATC 6 cut(s) 44, 125, 162, 244, 328, 390
AlwNI CAGNNNCTG 1 cut(s) 323
Aor13HI TCCGGA 1 cut(s) 218
Aor51HI AGCGCT 1 cut(s) 334
ApeKI GCWGC 3 cut(s) 24, 521, 524
AspLEI GCGC 1 cut(s) 335
AsuHPI GGTGA 1 cut(s) 84
BaeGI GKGCMC 1 cut(s) 181
BanI GGYRCC 2 cut(s) 176, 475
BbvI GCAGC 2 cut(s) 36, 533
BccI CCATC 1 cut(s) 142
BceAI ACGGC 3 cut(s) 12, 171, 311
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BciT130I CCWGG 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 508
BfaI CTAG 1 cut(s) 128
BfoI RGCGCY 1 cut(s) 336
BisI GCNGC 6 cut(s) 25, 146, 403, 445, 522, 525
BlsI GCNGC 6 cut(s) 26, 147, 404, 446, 523, 526
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 1 cut(s) 285
BmgBI CACGTC 1 cut(s) 440
BmiI GGNNCC 2 cut(s) 178, 477
BmrFI CCNGG 1 cut(s) 285
BsaBI GATNNNNATC 1 cut(s) 319
BsaI GGTCTC 1 cut(s) 508
BsaJI CCNNGG 1 cut(s) 14
BsaWI WCCGGW 2 cut(s) 218, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse8I GATNNNNATC 1 cut(s) 319
BseAI TCCGGA 1 cut(s) 218
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 14
BseJI GATNNNNATC 1 cut(s) 319
BseLI CCNNNNNNNGG 1 cut(s) 317
BseRI GAGGAG 1 cut(s) 289
BseSI GKGCMC 1 cut(s) 181
BseXI GCAGC 2 cut(s) 36, 533
Bsh1236I CGCG 1 cut(s) 444
BshNI GGYRCC 2 cut(s) 176, 475
BsiSI CCGG 2 cut(s) 219, 234
BslI CCNNNNNNNGG 1 cut(s) 317
BsmAI GTCTC 1 cut(s) 508
Bso31I GGTCTC 1 cut(s) 508
Bsp1286I GDGCHC 1 cut(s) 181
Bsp13I TCCGGA 1 cut(s) 218
Bsp143I GATC 8 cut(s) 49, 130, 154, 212, 236, 314, 320, 382
BspACI CCGC 4 cut(s) 145, 201, 403, 444
BspEI TCCGGA 1 cut(s) 218
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 2 cut(s) 178, 477
BspPI GGATC 6 cut(s) 44, 125, 162, 244, 328, 390
BspT107I GGYRCC 2 cut(s) 176, 475
BspTNI GGTCTC 1 cut(s) 508
BssECI CCNNGG 1 cut(s) 14
BssMI GATC 8 cut(s) 49, 130, 154, 212, 236, 314, 320, 382
Bst2UI CCWGG 1 cut(s) 285
BstFNI CGCG 1 cut(s) 444
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 8 cut(s) 52, 133, 157, 215, 239, 317, 323, 385
BstMAI GTCTC 1 cut(s) 508
BstMBI GATC 8 cut(s) 49, 130, 154, 212, 236, 314, 320, 382
BstMWI GCNNNNNNNGC 2 cut(s) 176, 332
BstNI CCWGG 1 cut(s) 285
BstSCI CCNGG 1 cut(s) 283
BstSLI GKGCMC 1 cut(s) 181
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 2 cut(s) 36, 533
BstX2I RGATCY 3 cut(s) 49, 130, 320
BstYI RGATCY 3 cut(s) 49, 130, 320
BtrI CACGTC 1 cut(s) 440
BtsIMutI CAGTG 1 cut(s) 256
CaiI CAGNNNCTG 1 cut(s) 323
CfoI GCGC 1 cut(s) 335
Csp6I GTAC 3 cut(s) 30, 44, 388
CviAII CATG 1 cut(s) 5
CviJI RGCY 8 cut(s) 27, 68, 104, 148, 170, 267, 402, 527
CviKI_1 RGCY 8 cut(s) 27, 68, 104, 148, 170, 267, 402, 527
CviQI GTAC 3 cut(s) 30, 44, 388
DpnI GATC 8 cut(s) 51, 132, 156, 214, 238, 316, 322, 384
DpnII GATC 8 cut(s) 49, 130, 154, 212, 236, 314, 320, 382
DrdI GACNNNNNNGTC 1 cut(s) 500
DseDI GACNNNNNNGTC 1 cut(s) 500
EciI GGCGGA 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 508
Eco32I GATATC 1 cut(s) 281
Eco47III AGCGCT 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 283
EcoRV GATATC 1 cut(s) 281
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 1 cut(s) 8
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FatI CATG 1 cut(s) 4
Fnu4HI GCNGC 6 cut(s) 25, 146, 403, 445, 522, 525
Fsp4HI GCNGC 6 cut(s) 25, 146, 403, 445, 522, 525
FspBI CTAG 1 cut(s) 128
GlaI GCGC 1 cut(s) 334
GluI GCNGC 6 cut(s) 25, 146, 403, 445, 522, 525
HaeII RGCGCY 1 cut(s) 336
HapII CCGG 2 cut(s) 219, 234
HhaI GCGC 1 cut(s) 335
Hin1II CATG 1 cut(s) 8
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HpaII CCGG 2 cut(s) 219, 234
HphI GGTGA 1 cut(s) 84
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 7 cut(s) 138, 219, 260, 318, 344, 380, 482
Hpy8I GTNNAC 1 cut(s) 254
Hpy99I CGWCG 1 cut(s) 444
HpyAV CCTTC 1 cut(s) 493
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 3 cut(s) 4, 290, 521
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 332
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 1 cut(s) 8
HspAI GCGC 1 cut(s) 333
Kpn2I TCCGGA 1 cut(s) 218
Kzo9I GATC 8 cut(s) 49, 130, 154, 212, 236, 314, 320, 382
LmnI GCTCC 1 cut(s) 414
Lsp1109I GCAGC 2 cut(s) 36, 533
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 439
MalI GATC 8 cut(s) 51, 132, 156, 214, 238, 316, 322, 384
MboI GATC 8 cut(s) 49, 130, 154, 212, 236, 314, 320, 382
MboII GAAGA 3 cut(s) 100, 234, 296
MflI RGATCY 3 cut(s) 49, 130, 320
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 1 cut(s) 159
MnlI CCTC 5 cut(s) 9, 197, 267, 466, 489
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 218
MspI CCGG 2 cut(s) 219, 234
MspR9I CCNGG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 285
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 2 cut(s) 176, 332
NdeII GATC 8 cut(s) 49, 130, 154, 212, 236, 314, 320, 382
NlaIII CATG 1 cut(s) 8
NlaIV GGNNCC 2 cut(s) 178, 477
NsiI ATGCAT 1 cut(s) 6
PkrI GCNGC 6 cut(s) 26, 147, 404, 446, 523, 526
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PspN4I GGNNCC 2 cut(s) 178, 477
PstNI CAGNNNCTG 1 cut(s) 323
PsuI RGATCY 3 cut(s) 49, 130, 320
RsaI GTAC 3 cut(s) 31, 45, 389
RsaNI GTAC 3 cut(s) 30, 44, 388
SatI GCNGC 6 cut(s) 25, 146, 403, 445, 522, 525
Sau3AI GATC 8 cut(s) 49, 130, 154, 212, 236, 314, 320, 382
ScaI AGTACT 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 285
SduI GDGCHC 1 cut(s) 181
SetI ASST 6 cut(s) 106, 442, 462, 477, 504, 529
Sse9I AATT 1 cut(s) 159
SsiI CCGC 4 cut(s) 145, 201, 403, 444
SspI AATATT 1 cut(s) 376
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 283
TaiI ACGT 1 cut(s) 442
TaqI TCGA 4 cut(s) 137, 157, 456, 471
TasI AATT 1 cut(s) 159
TatI WGTACW 1 cut(s) 387
TauI GCSGC 3 cut(s) 148, 405, 447
TscAI CASTG 1 cut(s) 256
TseI GCWGC 3 cut(s) 24, 521, 524
TspGWI ACGGA 1 cut(s) 182
TspRI CASTG 1 cut(s) 256
XspI CTAG 1 cut(s) 128
ZrmI AGTACT 1 cut(s) 389
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.