RLG00000026813

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000655
Physical Location & Seq
Forward (+)
6225976 .. 6229307
3332 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026813

Sequence Viewer

Length: 354 bp
ATGGCTCAATCAAGCTCGGAAGGTACTCCTAGTCAACGTCAGGATGAGATTGTGAAGAAATTTCCAGGGTCAAAGTTTATTCGTCGTCCAAATCCAGTGGAAGAAGCCGATGATTCAACTTACATTAAATTTCCAGAACATGACTGCGGGAATTATATGGAAAGATTCTATGAGAACCAGAGTCGCCGCCGGATCAGGTTGCATCCGGAGCTCGTGCATCCCAGCGACCACAGTTATGAAATTCCGGTGGAGGCGAAAGCGAGGACTGTAGCCGGTGAAGGAGAGCTTGTGATTGTTGGAGCTCGAGTCGAGGAAGTGAATCGGAGATTGGGGTCGTTGACCCGGTCGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

13.48

Weight (kDa)

6.43

Isoelectric Point (pI)

73.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 2 cut(s) 147, 187
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 3 cut(s) 59, 128, 240
AfaI GTAC 1 cut(s) 25
AfiI CCNNNNNNNGG 1 cut(s) 348
AgsI TTSAA 1 cut(s) 117
AjnI CCWGG 1 cut(s) 64
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 4 cut(s) 15, 211, 286, 302
AluI AGCT 4 cut(s) 15, 211, 286, 302
Alw21I GWGCWC 2 cut(s) 213, 304
AlwI GGATC 1 cut(s) 200
Ama87I CYCGRG 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 205
ApoI RAATTY 3 cut(s) 59, 128, 240
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 287
AvaI CYCGRG 1 cut(s) 303
BanII GRGCYC 2 cut(s) 213, 304
BauI CACGAG 1 cut(s) 212
Bbv12I GWGCWC 2 cut(s) 213, 304
BciT130I CCWGG 1 cut(s) 66
BcnI CCSGG 1 cut(s) 343
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 267
BisI GCNGC 1 cut(s) 187
BlsI GCNGC 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 66, 343
BmeT110I CYCGRG 1 cut(s) 303
BmrFI CCNGG 2 cut(s) 66, 343
BmsI GCATC 2 cut(s) 211, 226
BpuMI CCSGG 1 cut(s) 343
BsaJI CCNNGG 1 cut(s) 65
BsaWI WCCGGW 2 cut(s) 205, 244
BsaXI ACNNNNNCTCC 4 cut(s) 273, 291, 303, 321
Bsc4I CCNNNNNNNGG 1 cut(s) 348
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 95
BseAI TCCGGA 1 cut(s) 205
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 3 cut(s) 49, 202, 217
BseLI CCNNNNNNNGG 1 cut(s) 348
BseNI ACTGG 1 cut(s) 95
BseYI CCCAGC 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 347
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 2 cut(s) 213, 304
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 5 cut(s) 190, 206, 245, 273, 343
BslI CCNNNNNNNGG 1 cut(s) 348
BsoBI CYCGRG 1 cut(s) 303
Bsp1286I GDGCHC 2 cut(s) 213, 304
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 1 cut(s) 192
BspACI CCGC 2 cut(s) 147, 187
BspEI TCCGGA 1 cut(s) 205
BspPI GGATC 1 cut(s) 200
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 1 cut(s) 95
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 1 cut(s) 192
BssSI CACGAG 1 cut(s) 212
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 2 cut(s) 233, 268
BstF5I GGATG 3 cut(s) 49, 202, 217
BstKTI GATC 1 cut(s) 195
BstMBI GATC 1 cut(s) 192
BstMCI CGRYCG 1 cut(s) 347
BstMWI GCNNNNNNNGC 1 cut(s) 208
BstNI CCWGG 1 cut(s) 66
BstSCI CCNGG 2 cut(s) 64, 341
BstSFI CTRYAG 1 cut(s) 267
BtsCI GGATG 3 cut(s) 49, 202, 217
BtsIMutI CAGTG 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 272
Csp6I GTAC 1 cut(s) 24
CspCI CAANNNNNGTGG 2 cut(s) 78, 113
CviAII CATG 1 cut(s) 140
CviJI RGCY 7 cut(s) 5, 15, 107, 211, 272, 286, 302
CviKI_1 RGCY 7 cut(s) 5, 15, 107, 211, 272, 286, 302
CviQI GTAC 1 cut(s) 24
DpnI GATC 1 cut(s) 194
DpnII GATC 1 cut(s) 192
Ecl136II GAGCTC 2 cut(s) 211, 302
Eco24I GRGCYC 2 cut(s) 213, 304
Eco53kI GAGCTC 2 cut(s) 211, 302
Eco88I CYCGRG 1 cut(s) 303
EcoICRI GAGCTC 2 cut(s) 211, 302
EcoRII CCWGG 1 cut(s) 64
EcoT38I GRGCYC 2 cut(s) 213, 304
FaeI CATG 1 cut(s) 143
FaiI YATR 5 cut(s) 141, 156, 158, 171, 237
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FatI CATG 1 cut(s) 139
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 1 cut(s) 187
FokI GGATG 3 cut(s) 56, 189, 204
FriOI GRGCYC 2 cut(s) 213, 304
Fsp4HI GCNGC 1 cut(s) 187
FspBI CTAG 1 cut(s) 30
GluI GCNGC 1 cut(s) 187
GsaI CCCAGC 1 cut(s) 225
HapII CCGG 5 cut(s) 190, 206, 245, 273, 343
Hin1II CATG 1 cut(s) 143
HincII GTYRAC 2 cut(s) 35, 339
HindII GTYRAC 2 cut(s) 35, 339
HinfI GANTC 5 cut(s) 113, 165, 181, 306, 319
HpaII CCGG 5 cut(s) 190, 206, 245, 273, 343
HphI GGTGA 1 cut(s) 287
Hpy166II GTNNAC 2 cut(s) 35, 339
Hpy188I TCNGA 2 cut(s) 19, 324
Hpy188III TCNNGA 3 cut(s) 41, 134, 206
Hpy8I GTNNAC 2 cut(s) 35, 339
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 2 cut(s) 14, 272
HpyCH4III ACNGT 2 cut(s) 233, 268
HpyCH4IV ACGT 1 cut(s) 37
HpyCH4V TGCA 2 cut(s) 202, 217
HpyF10VI GCNNNNNNNGC 1 cut(s) 208
HpySE526I ACGT 1 cut(s) 37
Hsp92II CATG 1 cut(s) 143
Kpn2I TCCGGA 1 cut(s) 205
Kzo9I GATC 1 cut(s) 192
LmnI GCTCC 2 cut(s) 208, 299
LweI GCATC 2 cut(s) 211, 226
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 37
MalI GATC 1 cut(s) 194
MboI GATC 1 cut(s) 192
MboII GAAGA 2 cut(s) 67, 113
MhlI GDGCHC 2 cut(s) 213, 304
MluCI AATT 4 cut(s) 59, 128, 151, 240
MlyI GAGTC 2 cut(s) 190, 315
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 3 cut(s) 244, 255, 304
MroI TCCGGA 1 cut(s) 205
MseI TTAA 1 cut(s) 126
MslI CAYNNNNRTG 1 cut(s) 234
MspI CCGG 5 cut(s) 190, 206, 245, 273, 343
MspR9I CCNGG 2 cut(s) 66, 343
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 1 cut(s) 208
NciI CCSGG 1 cut(s) 343
NdeII GATC 1 cut(s) 192
NlaIII CATG 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 303
PfeI GAWTC 3 cut(s) 113, 165, 319
PflFI GACNNNGTC 1 cut(s) 343
PkrI GCNGC 1 cut(s) 188
PleI GAGTC 2 cut(s) 189, 314
PpsI GAGTC 2 cut(s) 189, 314
Psp124BI GAGCTC 2 cut(s) 213, 304
Psp6I CCWGG 1 cut(s) 64
PspFI CCCAGC 1 cut(s) 221
PspGI CCWGG 1 cut(s) 64
PspXI VCTCGAGB 1 cut(s) 303
PsyI GACNNNGTC 1 cut(s) 343
RsaI GTAC 1 cut(s) 25
RsaNI GTAC 1 cut(s) 24
RseI CAYNNNNRTG 1 cut(s) 234
SacI GAGCTC 2 cut(s) 213, 304
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 1 cut(s) 187
Sau3AI GATC 1 cut(s) 192
SchI GAGTC 2 cut(s) 190, 315
ScrFI CCNGG 2 cut(s) 66, 343
SduI GDGCHC 2 cut(s) 213, 304
SetI ASST 7 cut(s) 17, 25, 40, 200, 213, 288, 304
SfaNI GCATC 2 cut(s) 211, 226
SfcI CTRYAG 1 cut(s) 267
Sfr274I CTCGAG 1 cut(s) 303
SlaI CTCGAG 1 cut(s) 303
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
Sse9I AATT 4 cut(s) 59, 128, 151, 240
SsiI CCGC 2 cut(s) 147, 187
SspMI CTAG 1 cut(s) 30
SstI GAGCTC 2 cut(s) 213, 304
StyD4I CCNGG 2 cut(s) 64, 341
TaaI ACNGT 2 cut(s) 233, 268
TaiI ACGT 1 cut(s) 40
TaqI TCGA 2 cut(s) 304, 309
TasI AATT 4 cut(s) 59, 128, 151, 240
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 3 cut(s) 113, 165, 319
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 1 cut(s) 102
TspDTI ATGAA 1 cut(s) 252
TspRI CASTG 1 cut(s) 102
Tth111I GACNNNGTC 1 cut(s) 343
XapI RAATTY 3 cut(s) 59, 128, 240
XhoI CTCGAG 1 cut(s) 303
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.