Rorug01G0380200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000655
Physical Location & Seq
Forward (+)
49297945 .. 49298969
1025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0380200.1

Sequence Viewer

Length: 528 bp
ATGGATCAGAACAAGAAGCAAGTCGGTACGACAAGCCATAGCCATGGCTCTTCTTCTTCCTCTTCATCCACGAACCTTGACCATCTCTTTGGTCCTAAGGACTCGTCGTCTTCAGCTTCTTCATCTCTATTCGGCTCTATTTTCCCACCACCACCATCCACGGGACCGGGGAGAGAAAGAAACTCTGGTAATACTACTCGAGGCAGCAGCAAGGGTTCCGTGCATCAGAATAATGAAGTTGTGGATCGTCCTTGCAACTTTAACTCATCAATCTACTATGGCGGCCAAGAAAACTATAATGTTCCGAAAAGTGGGAGTACTTACGAGTCCCAGCACCACCAGCATAACTCTAAGAAAGACGGGGTGGATGATGATTCGAATGGAAATAATTCAAACAGTACTTCAAGAGGAAACTGGTGGAAGGTGATCAACATTATTCGTGGTCACTTTATTACTAAATCAGTAAATCTTGCTGCACGTACTATGCGAATTCACCACCCTTTGAAAGAGGTGTGTGTCACTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

18.99

Weight (kDa)

9.36

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 282
AclWI GGATC 2 cut(s) 12, 252
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 489
AcuI CTGAAG 1 cut(s) 96
AfaI GTAC 4 cut(s) 28, 319, 400, 481
AfiI CCNNNNNNNGG 2 cut(s) 161, 311
AgsI TTSAA 3 cut(s) 393, 405, 505
AhdI GACNNNNNGTC 1 cut(s) 106
AluBI AGCT 1 cut(s) 116
AluI AGCT 1 cut(s) 116
AlwI GGATC 2 cut(s) 12, 252
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 1 cut(s) 283
ApeKI GCWGC 3 cut(s) 204, 207, 473
ApoI RAATTY 1 cut(s) 489
ArsI GACNNNNNNTTYG 2 cut(s) 71, 103
Asp700I GAANNNNTTC 1 cut(s) 388
AspS9I GGNCC 2 cut(s) 92, 164
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 2 cut(s) 436, 485
AsuII TTCGAA 1 cut(s) 377
AvaI CYCGRG 1 cut(s) 198
AvaII GGWCC 2 cut(s) 92, 164
AxyI CCTNAGG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 102
BbvI GCAGC 3 cut(s) 216, 219, 460
BccI CCATC 2 cut(s) 90, 163
BclI TGATCA 1 cut(s) 426
BcnI CCSGG 1 cut(s) 168
BisI GCNGC 4 cut(s) 205, 208, 283, 474
BlsI GCNGC 4 cut(s) 206, 209, 284, 475
BmcAI AGTACT 2 cut(s) 319, 400
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 92, 164
BmeRI GACNNNNNGTC 1 cut(s) 106
BmeT110I CYCGRG 1 cut(s) 198
BmgT120I GGNCC 2 cut(s) 92, 164
BmiI GGNNCC 2 cut(s) 165, 217
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 377
BpuMI CCSGG 1 cut(s) 168
BsaAI YACGTR 1 cut(s) 479
BsaJI CCNNGG 3 cut(s) 43, 159, 167
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 311
Bse1I ACTGG 1 cut(s) 419
Bse21I CCTNAGG 1 cut(s) 96
BseDI CCNNGG 3 cut(s) 43, 159, 167
BseGI GGATG 3 cut(s) 65, 155, 373
BseLI CCNNNNNNNGG 2 cut(s) 161, 311
BseNI ACTGG 1 cut(s) 419
BseXI GCAGC 3 cut(s) 216, 219, 460
BseYI CCCAGC 1 cut(s) 330
BsgI GTGCAG 1 cut(s) 459
BshFI GGCC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 1 cut(s) 167
BslFI GGGAC 2 cut(s) 177, 313
BslI CCNNNNNNNGG 2 cut(s) 161, 311
BsmFI GGGAC 2 cut(s) 177, 313
BsnI GGCC 1 cut(s) 285
BsoBI CYCGRG 1 cut(s) 198
Bsp119I TTCGAA 1 cut(s) 377
Bsp143I GATC 3 cut(s) 4, 244, 426
Bsp19I CCATGG 1 cut(s) 43
BspACI CCGC 1 cut(s) 282
BspANI GGCC 1 cut(s) 285
BspLI GGNNCC 2 cut(s) 165, 217
BspPI GGATC 2 cut(s) 12, 252
BspQI GCTCTTC 1 cut(s) 55
BspT104I TTCGAA 1 cut(s) 377
BsrI ACTGG 1 cut(s) 419
BssECI CCNNGG 3 cut(s) 43, 159, 167
BssMI GATC 3 cut(s) 4, 244, 426
BssT1I CCWWGG 1 cut(s) 43
Bst4CI ACNGT 1 cut(s) 398
Bst6I CTCTTC 2 cut(s) 55, 67
BstBAI YACGTR 1 cut(s) 479
BstBI TTCGAA 1 cut(s) 377
BstDEI CTNAG 2 cut(s) 96, 351
BstDSI CCRYGG 2 cut(s) 43, 159
BstF5I GGATG 3 cut(s) 65, 155, 373
BstKTI GATC 3 cut(s) 7, 247, 429
BstMBI GATC 3 cut(s) 4, 244, 426
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstSCI CCNGG 1 cut(s) 166
BstV1I GCAGC 3 cut(s) 216, 219, 460
BstV2I GAAGAC 1 cut(s) 102
BstXI CCANNNNNNTGG 2 cut(s) 44, 89
Bsu36I CCTNAGG 1 cut(s) 96
BsuRI GGCC 1 cut(s) 285
BtgI CCRYGG 2 cut(s) 43, 159
BtsCI GGATG 3 cut(s) 65, 155, 373
Cfr13I GGNCC 2 cut(s) 92, 164
Csp6I GTAC 4 cut(s) 27, 318, 399, 480
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 1 cut(s) 44
CviJI RGCY 6 cut(s) 36, 42, 48, 116, 135, 285
CviKI_1 RGCY 6 cut(s) 36, 42, 48, 116, 135, 285
CviQI GTAC 4 cut(s) 27, 318, 399, 480
DdeI CTNAG 2 cut(s) 96, 351
DpnI GATC 3 cut(s) 6, 246, 428
DpnII GATC 3 cut(s) 4, 244, 426
DriI GACNNNNNGTC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 283
Eam1104I CTCTTC 2 cut(s) 55, 67
Eam1105I GACNNNNNGTC 1 cut(s) 106
EarI CTCTTC 2 cut(s) 55, 67
Eco130I CCWWGG 1 cut(s) 43
Eco47I GGWCC 2 cut(s) 92, 164
Eco57I CTGAAG 1 cut(s) 96
Eco81I CCTNAGG 1 cut(s) 96
Eco88I CYCGRG 1 cut(s) 198
EcoRI GAATTC 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 1 cut(s) 47
FaiI YATR 7 cut(s) 39, 45, 279, 297, 345, 485, 526
FaqI GGGAC 2 cut(s) 177, 313
FatI CATG 1 cut(s) 43
FbaI TGATCA 1 cut(s) 426
Fnu4HI GCNGC 4 cut(s) 205, 208, 283, 474
FokI GGATG 3 cut(s) 52, 142, 380
Fsp4HI GCNGC 4 cut(s) 205, 208, 283, 474
GluI GCNGC 4 cut(s) 205, 208, 283, 474
GsaI CCCAGC 1 cut(s) 334
HaeIII GGCC 1 cut(s) 285
HapII CCGG 1 cut(s) 167
Hin1II CATG 1 cut(s) 47
HinfI GANTC 3 cut(s) 101, 326, 374
HpaII CCGG 1 cut(s) 167
HphI GGTGA 2 cut(s) 436, 485
Hpy188I TCNGA 3 cut(s) 9, 228, 306
Hpy188III TCNNGA 1 cut(s) 405
Hpy99I CGWCG 1 cut(s) 109
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 1 cut(s) 398
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 3 cut(s) 223, 255, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpyF3I CTNAG 2 cut(s) 96, 351
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 426
Kzo9I GATC 3 cut(s) 4, 244, 426
LguI GCTCTTC 1 cut(s) 55
LpnPI CCDG 5 cut(s) 171, 180, 344, 353, 400
Lsp1109I GCAGC 3 cut(s) 216, 219, 460
LweI GCATC 1 cut(s) 232
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 2 cut(s) 443, 517
MalI GATC 3 cut(s) 6, 246, 428
MboI GATC 3 cut(s) 4, 244, 426
MboII GAAGA 6 cut(s) 42, 45, 48, 54, 102, 111
MluCI AATT 2 cut(s) 388, 489
MlyI GAGTC 2 cut(s) 95, 335
MnlI CCTC 4 cut(s) 70, 194, 401, 502
MroXI GAANNNNTTC 1 cut(s) 388
MseI TTAA 1 cut(s) 261
MslI CAYNNNNRTG 1 cut(s) 42
MspI CCGG 1 cut(s) 167
MspR9I CCNGG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 340
NciI CCSGG 1 cut(s) 168
NcoI CCATGG 1 cut(s) 43
NdeII GATC 3 cut(s) 4, 244, 426
NlaIII CATG 1 cut(s) 47
NlaIV GGNNCC 2 cut(s) 165, 217
NmuCI GTSAC 2 cut(s) 443, 517
NspV TTCGAA 1 cut(s) 377
PaeR7I CTCGAG 1 cut(s) 198
PciSI GCTCTTC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 484
PdmI GAANNNNTTC 1 cut(s) 388
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 4 cut(s) 206, 209, 284, 475
PleI GAGTC 2 cut(s) 95, 334
PpsI GAGTC 2 cut(s) 95, 334
Ppu21I YACGTR 1 cut(s) 479
PspFI CCCAGC 1 cut(s) 330
PspN4I GGNNCC 2 cut(s) 165, 217
PspPI GGNCC 2 cut(s) 92, 164
PspXI VCTCGAGB 1 cut(s) 198
RsaI GTAC 4 cut(s) 28, 319, 400, 481
RsaNI GTAC 4 cut(s) 27, 318, 399, 480
RseI CAYNNNNRTG 1 cut(s) 42
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 261
SatI GCNGC 4 cut(s) 205, 208, 283, 474
Sau3AI GATC 3 cut(s) 4, 244, 426
Sau96I GGNCC 2 cut(s) 92, 164
ScaI AGTACT 2 cut(s) 319, 400
SchI GAGTC 2 cut(s) 95, 335
ScrFI CCNGG 1 cut(s) 168
SetI ASST 5 cut(s) 78, 118, 426, 481, 513
SfaNI GCATC 1 cut(s) 232
Sfr274I CTCGAG 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 377
SinI GGWCC 2 cut(s) 92, 164
SlaI CTCGAG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 42
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
Sse9I AATT 2 cut(s) 388, 489
SsiI CCGC 1 cut(s) 282
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 1 cut(s) 398
TaiI ACGT 1 cut(s) 481
TaqI TCGA 2 cut(s) 199, 377
TasI AATT 2 cut(s) 388, 489
TatI WGTACW 2 cut(s) 317, 398
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 1 cut(s) 261
Tru9I TTAA 1 cut(s) 261
TseFI GTSAC 2 cut(s) 443, 517
TseI GCWGC 3 cut(s) 204, 207, 473
Tsp45I GTSAC 2 cut(s) 443, 517
TspDTI ATGAA 3 cut(s) 54, 111, 249
TspGWI ACGGA 1 cut(s) 208
VpaK11BI GGWCC 2 cut(s) 92, 164
XapI RAATTY 1 cut(s) 489
XhoI CTCGAG 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 388
ZrmI AGTACT 2 cut(s) 319, 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.