FvH4_7g27070

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000655
Physical Location & Seq
Forward (+)
20310662 .. 20311781
1120 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27070.t1

Sequence Viewer

Length: 384 bp
ATGGGTCGCAATCGTTCTCGTGCAGGAGGAGGAGGAGGATCGCTTACCAGAAGAGGAAGATCGCTTAATTACAGAAGTGCAGGTTATGGCTTGAGTACCAGCCAGCAACAAGAGGCTCTTATCAAGAATTCTCCTAATCCCGGGGCTGCTTATATCGTTCATGGTTCACAGATATCTCAGGGTAATAGATATCCGTATGATCAATGCGAAAGATTTCTCAATTACCTCTATGATGTAACACGATTGTGTGGGAGCGATTGTCACCTTGCTCACGCATATAGAGATAGCACAAGGATCTTTTGTGGTATTGATGTGTTAACGGATCATGAATTAGAGAAGAAGAAGAAGAAGGCCACTGATGCAGCAGCAGCAGCTCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

13.95

Weight (kDa)

9.44

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 71
AclWI GGATC 3 cut(s) 46, 302, 330
AcsI RAATTY 1 cut(s) 127
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 1 cut(s) 140
AleI CACNNNNGTG 1 cut(s) 244
AluBI AGCT 1 cut(s) 374
AluI AGCT 1 cut(s) 374
AlwI GGATC 3 cut(s) 46, 302, 330
Ama87I CYCGRG 1 cut(s) 140
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 5 cut(s) 146, 362, 365, 368, 371
ApoI RAATTY 1 cut(s) 127
Asp700I GAANNNNTTC 1 cut(s) 213
AsuC2I CCSGG 2 cut(s) 141, 142
AsuHPI GGTGA 1 cut(s) 254
AvaI CYCGRG 1 cut(s) 140
BauI CACGAG 1 cut(s) 18
BbvI GCAGC 4 cut(s) 133, 374, 377, 380
BclI TGATCA 1 cut(s) 199
BcnI CCSGG 2 cut(s) 141, 142
BfuAI ACCTGC 1 cut(s) 71
BisI GCNGC 5 cut(s) 147, 363, 366, 369, 372
BlsI GCNGC 5 cut(s) 148, 364, 367, 370, 373
Bme1390I CCNGG 2 cut(s) 141, 142
BmeT110I CYCGRG 1 cut(s) 140
BmrFI CCNGG 2 cut(s) 141, 142
BmsI GCATC 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 112
BpuMI CCSGG 2 cut(s) 141, 142
BsaJI CCNNGG 2 cut(s) 140, 141
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 140
BseDI CCNNGG 2 cut(s) 140, 141
BseLI CCNNNNNNNGG 1 cut(s) 140
BseMII CTCAG 1 cut(s) 191
BseRI GAGGAG 3 cut(s) 42, 45, 48
BseXI GCAGC 4 cut(s) 133, 374, 377, 380
BsgI GTGCAG 2 cut(s) 42, 99
BshFI GGCC 1 cut(s) 353
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 140
BsnI GGCC 1 cut(s) 353
BsoBI CYCGRG 1 cut(s) 140
Bsp143I GATC 5 cut(s) 38, 59, 199, 294, 322
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 1 cut(s) 190
BspHI TCATGA 1 cut(s) 325
BspMI ACCTGC 1 cut(s) 71
BspPI GGATC 3 cut(s) 46, 302, 330
BssECI CCNNGG 2 cut(s) 140, 141
BssMI GATC 5 cut(s) 38, 59, 199, 294, 322
BssSI CACGAG 1 cut(s) 18
Bst2BI CACGAG 1 cut(s) 18
Bst6I CTCTTC 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 1 cut(s) 177
BstKTI GATC 5 cut(s) 41, 62, 202, 297, 325
BstMBI GATC 5 cut(s) 38, 59, 199, 294, 322
BstMWI GCNNNNNNNGC 3 cut(s) 359, 368, 371
BstSCI CCNGG 2 cut(s) 139, 140
BstV1I GCAGC 4 cut(s) 133, 374, 377, 380
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuRI GGCC 1 cut(s) 353
BtsIMutI CAGTG 1 cut(s) 354
BveI ACCTGC 1 cut(s) 71
Cac8I GCNNGC 1 cut(s) 104
CciI TCATGA 1 cut(s) 325
Cfr9I CCCGGG 1 cut(s) 140
Csp6I GTAC 1 cut(s) 96
CviAII CATG 2 cut(s) 161, 326
CviJI RGCY 6 cut(s) 90, 102, 116, 146, 353, 374
CviKI_1 RGCY 6 cut(s) 90, 102, 116, 146, 353, 374
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 1 cut(s) 177
DpnI GATC 5 cut(s) 40, 61, 201, 296, 324
DpnII GATC 5 cut(s) 38, 59, 199, 294, 322
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
Eco32I GATATC 2 cut(s) 174, 191
Eco88I CYCGRG 1 cut(s) 140
EcoRI GAATTC 1 cut(s) 127
EcoRV GATATC 2 cut(s) 174, 191
FaeI CATG 2 cut(s) 164, 329
FaiI YATR 8 cut(s) 87, 153, 162, 198, 231, 277, 279, 327
FalI AAGNNNNNCTT 2 cut(s) 102, 134
FatI CATG 2 cut(s) 160, 325
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 5 cut(s) 147, 363, 366, 369, 372
Fsp4HI GCNGC 5 cut(s) 147, 363, 366, 369, 372
GluI GCNGC 5 cut(s) 147, 363, 366, 369, 372
HaeIII GGCC 1 cut(s) 353
HapII CCGG 1 cut(s) 141
Hin1II CATG 2 cut(s) 164, 329
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HpaI GTTAAC 1 cut(s) 318
HpaII CCGG 1 cut(s) 141
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 2 cut(s) 167, 318
Hpy188III TCNNGA 2 cut(s) 124, 326
Hpy8I GTNNAC 2 cut(s) 167, 318
HpyAV CCTTC 1 cut(s) 343
HpyCH4V TGCA 3 cut(s) 23, 80, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 359, 368, 371
HpyF3I CTNAG 1 cut(s) 177
Hsp92II CATG 2 cut(s) 164, 329
Ksp22I TGATCA 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 318
Kzo9I GATC 5 cut(s) 38, 59, 199, 294, 322
LmnI GCTCC 1 cut(s) 252
LpnPI CCDG 7 cut(s) 9, 61, 66, 112, 116, 154, 164
Lsp1109I GCAGC 4 cut(s) 133, 374, 377, 380
LweI GCATC 1 cut(s) 349
MaeIII GTNAC 2 cut(s) 235, 260
MalI GATC 5 cut(s) 40, 61, 201, 296, 324
MboI GATC 5 cut(s) 38, 59, 199, 294, 322
MboII GAAGA 6 cut(s) 63, 69, 349, 352, 355, 358
MflI RGATCY 1 cut(s) 294
MluCI AATT 4 cut(s) 67, 127, 220, 329
MnlI CCTC 7 cut(s) 20, 23, 26, 29, 47, 106, 236
MroXI GAANNNNTTC 1 cut(s) 213
MseI TTAA 2 cut(s) 66, 317
MslI CAYNNNNRTG 1 cut(s) 244
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 2 cut(s) 141, 142
MwoI GCNNNNNNNGC 3 cut(s) 359, 368, 371
NciI CCSGG 2 cut(s) 141, 142
NdeII GATC 5 cut(s) 38, 59, 199, 294, 322
NlaIII CATG 2 cut(s) 164, 329
NmuCI GTSAC 1 cut(s) 260
OliI CACNNNNGTG 1 cut(s) 244
PagI TCATGA 1 cut(s) 325
PdmI GAANNNNTTC 1 cut(s) 213
PkrI GCNGC 5 cut(s) 148, 364, 367, 370, 373
PsuI RGATCY 1 cut(s) 294
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
RseI CAYNNNNRTG 1 cut(s) 244
SaqAI TTAA 2 cut(s) 66, 317
SatI GCNGC 5 cut(s) 147, 363, 366, 369, 372
Sau3AI GATC 5 cut(s) 38, 59, 199, 294, 322
ScrFI CCNGG 2 cut(s) 141, 142
SetI ASST 4 cut(s) 85, 228, 267, 376
SfaNI GCATC 1 cut(s) 349
SmaI CCCGGG 1 cut(s) 142
SmiMI CAYNNNNRTG 1 cut(s) 244
SmlI CTYRAG 1 cut(s) 91
SmoI CTYRAG 1 cut(s) 91
Sse9I AATT 4 cut(s) 67, 127, 220, 329
StyD4I CCNGG 2 cut(s) 139, 140
TasI AATT 4 cut(s) 67, 127, 220, 329
Tru1I TTAA 2 cut(s) 66, 317
Tru9I TTAA 2 cut(s) 66, 317
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 5 cut(s) 146, 362, 365, 368, 371
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 2 cut(s) 149, 342
TspGWI ACGGA 2 cut(s) 183, 335
TspMI CCCGGG 1 cut(s) 140
TspRI CASTG 1 cut(s) 361
XapI RAATTY 1 cut(s) 127
XmaI CCCGGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.