Rmu_sc0000475.1_g000009

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000475.1
OG0000655
Physical Location & Seq
Reverse (-)
49863 .. 50831
969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000475.1_g000009.1.cds

Sequence Viewer

Length: 405 bp
atggctggtggtgcccgttgcggtgtccgttcaggcggaggtttgagatcgtccggaagaattggaggaaaatccactggcctaaggtcaggtcccagattaagtaccagtgaacaacaagaagaccttgtgaggagatatcctggtgcagcttatattgttactcgtagaagatcagaacctaccgctggcgctggcgctgtggcttctgacgaaaattatccgtttgattactgccagaggcttctggattactactatgatacaacacgcttgtgtgggagcgactataccgatagtgtattatatcgcaacgctatacttaatatttgtggtattcaggtgtcacctgatgagttagacaagaaggcctctacttccaccaccgctgcagctcatctttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

134

Amino Acids

14.38

Weight (kDa)

8.73

Isoelectric Point (pI)

60.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 11
AccIII TCCGGA 1 cut(s) 53
AciI CCGC 4 cut(s) 21, 36, 186, 387
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 1 cut(s) 188
AjnI CCWGG 1 cut(s) 142
AleI CACNNNNGTG 1 cut(s) 274
AluBI AGCT 2 cut(s) 152, 395
AluI AGCT 2 cut(s) 152, 395
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 2 cut(s) 79, 369
ApeKI GCWGC 3 cut(s) 149, 389, 392
AspLEI GCGC 2 cut(s) 194, 200
AspS9I GGNCC 1 cut(s) 92
AsuHPI GGTGA 1 cut(s) 339
AvaII GGWCC 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 83
BaeGI GKGCMC 1 cut(s) 16
BanI GGYRCC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 129
BbvI GCAGC 2 cut(s) 161, 376
BciT130I CCWGG 1 cut(s) 144
BfmI CTRYAG 1 cut(s) 390
BfoI RGCGCY 2 cut(s) 195, 201
BisI GCNGC 3 cut(s) 150, 390, 393
BlsI GCNGC 3 cut(s) 151, 391, 394
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 1 cut(s) 92
BmiI GGNNCC 2 cut(s) 13, 94
BmrFI CCNGG 1 cut(s) 144
BpiI GAAGAC 1 cut(s) 129
BsaWI WCCGGW 1 cut(s) 53
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse1I ACTGG 2 cut(s) 82, 108
Bse21I CCTNAGG 1 cut(s) 83
BseAI TCCGGA 1 cut(s) 53
BseBI CCWGG 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 188
BseNI ACTGG 2 cut(s) 82, 108
BseRI GAGGAG 1 cut(s) 148
BseSI GKGCMC 1 cut(s) 16
BseXI GCAGC 2 cut(s) 161, 376
BsgI GTGCAG 1 cut(s) 168
BshFI GGCC 2 cut(s) 81, 371
BshNI GGYRCC 1 cut(s) 11
BsiSI CCGG 1 cut(s) 54
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 1 cut(s) 188
BsmFI GGGAC 1 cut(s) 78
BsnI GGCC 2 cut(s) 81, 371
Bsp1286I GDGCHC 1 cut(s) 16
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 2 cut(s) 47, 173
BspACI CCGC 4 cut(s) 21, 36, 186, 387
BspANI GGCC 2 cut(s) 81, 371
BspEI TCCGGA 1 cut(s) 53
BspLI GGNNCC 2 cut(s) 13, 94
BspMAI CTGCAG 1 cut(s) 394
BspT107I GGYRCC 1 cut(s) 11
BsrI ACTGG 2 cut(s) 82, 108
BssMI GATC 2 cut(s) 47, 173
Bst2UI CCWGG 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 190, 196
BstDEI CTNAG 1 cut(s) 83
BstH2I RGCGCY 2 cut(s) 195, 201
BstHHI GCGC 2 cut(s) 194, 200
BstKTI GATC 2 cut(s) 50, 176
BstMBI GATC 2 cut(s) 47, 173
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 16
BstV1I GCAGC 2 cut(s) 161, 376
BstV2I GAAGAC 1 cut(s) 129
Bsu36I CCTNAGG 1 cut(s) 83
BsuRI GGCC 2 cut(s) 81, 371
BtsIMutI CAGTG 2 cut(s) 75, 115
Cac8I GCNNGC 2 cut(s) 190, 196
CfoI GCGC 2 cut(s) 194, 200
Cfr13I GGNCC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 105
CviJI RGCY 7 cut(s) 5, 81, 152, 206, 244, 371, 395
CviKI_1 RGCY 7 cut(s) 5, 81, 152, 206, 244, 371, 395
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 1 cut(s) 83
DpnI GATC 2 cut(s) 49, 175
DpnII GATC 2 cut(s) 47, 173
EciI GGCGGA 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 371
Eco32I GATATC 1 cut(s) 140
Eco47I GGWCC 1 cut(s) 92
Eco81I CCTNAGG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 142
EcoRV GATATC 1 cut(s) 140
FaiI YATR 5 cut(s) 156, 261, 291, 307, 320
FalI AAGNNNNNCTT 2 cut(s) 111, 143
FaqI GGGAC 1 cut(s) 78
Fnu4HI GCNGC 3 cut(s) 150, 390, 393
Fsp4HI GCNGC 3 cut(s) 150, 390, 393
GlaI GCGC 2 cut(s) 193, 199
GluI GCNGC 3 cut(s) 150, 390, 393
HaeII RGCGCY 2 cut(s) 195, 201
HaeIII GGCC 2 cut(s) 81, 371
HapII CCGG 1 cut(s) 54
HhaI GCGC 2 cut(s) 194, 200
Hin6I GCGC 2 cut(s) 192, 198
HinP1I GCGC 2 cut(s) 192, 198
HpaII CCGG 1 cut(s) 54
HphI GGTGA 1 cut(s) 339
Hpy166II GTNNAC 1 cut(s) 113
Hpy188I TCNGA 2 cut(s) 178, 211
Hpy188III TCNNGA 2 cut(s) 54, 248
Hpy8I GTNNAC 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 361
HpyCH4V TGCA 2 cut(s) 149, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 83
HspAI GCGC 2 cut(s) 192, 198
Kpn2I TCCGGA 1 cut(s) 53
Kzo9I GATC 2 cut(s) 47, 173
LmnI GCTCC 1 cut(s) 282
Lsp1109I GCAGC 2 cut(s) 161, 376
MaeIII GTNAC 2 cut(s) 160, 345
MalI GATC 2 cut(s) 49, 175
MboI GATC 2 cut(s) 47, 173
MboII GAAGA 3 cut(s) 69, 134, 183
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 2 cut(s) 60, 217
MnlI CCTC 5 cut(s) 32, 59, 126, 234, 382
MroI TCCGGA 1 cut(s) 53
MseI TTAA 2 cut(s) 101, 324
MslI CAYNNNNRTG 1 cut(s) 274
MspA1I CMGCKG 2 cut(s) 188, 389
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 11
NdeII GATC 2 cut(s) 47, 173
NlaIV GGNNCC 2 cut(s) 13, 94
NmuCI GTSAC 1 cut(s) 345
OliI CACNNNNGTG 1 cut(s) 274
PceI AGGCCT 1 cut(s) 371
PkrI GCNGC 3 cut(s) 151, 391, 394
PpuMI RGGWCCY 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 2 cut(s) 13, 94
PspPI GGNCC 1 cut(s) 92
PspPPI RGGWCCY 1 cut(s) 92
PstI CTGCAG 1 cut(s) 394
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
RseI CAYNNNNRTG 1 cut(s) 274
SaqAI TTAA 2 cut(s) 101, 324
SatI GCNGC 3 cut(s) 150, 390, 393
Sau3AI GATC 2 cut(s) 47, 173
Sau96I GGNCC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 1 cut(s) 16
SetI ASST 9 cut(s) 43, 89, 94, 129, 154, 184, 345, 352, 397
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 274
Sse9I AATT 2 cut(s) 60, 217
SseBI AGGCCT 1 cut(s) 371
SsiI CCGC 4 cut(s) 21, 36, 186, 387
SspI AATATT 1 cut(s) 328
StuI AGGCCT 1 cut(s) 371
StyD4I CCNGG 1 cut(s) 142
TasI AATT 2 cut(s) 60, 217
Tru1I TTAA 2 cut(s) 101, 324
Tru9I TTAA 2 cut(s) 101, 324
TscAI CASTG 2 cut(s) 82, 115
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 3 cut(s) 149, 389, 392
Tsp45I GTSAC 1 cut(s) 345
TspGWI ACGGA 2 cut(s) 17, 213
TspRI CASTG 2 cut(s) 82, 115
VpaK11BI GGWCC 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.