FvH4_2g29544

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000509
Physical Location & Seq
Reverse (-)
22888658 .. 22892139
3482 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29544.t1

Sequence Viewer

Length: 2346 bp
ATGTGGTCCAAGGAGCTTGAATCAAGTGGTGATCAGCTTTGCACTCCTGCAGTCATGCTTGTTCCTTTAATTTGGTTGATATCATCATCTAGCTTCTTTACATTTTCATCTGCAACAGACACTCTTACACCGTCAGACTCATTGAGGGACAATCAGGTGCTGGTTTCAGCTGGTGGAGTGTTTGAGCTTGGCTTATTCAATGAAAGCATTTCAGGCTATTACTATCTGGGGATTTGGTTGAGAGCTGATCCAACCAGGATTGTCTGGGTTGCTCGTGATAATCCCATACTGGATTCATCTGGTCTACTTCAAATCCTGTCTGGGAATTTGGTTCTCACAGACCGTCGACAAGTGCAATTGATACTCAACTCAGGAAGTCTTGCCAAGGCCACAACTGCTAACACAAGTGCAACACTTCTTGATACTGGAAATTTTGTCCTTAAGGAAGTGGATACAGGTAATACTATATGGCAAAGTTTTGATGTAGCGAGTGATACTTACCTTCCTGGTATGAAGCTTGGGTTGTTTGGACAAGAGCTTCATAATATTGTTTCTTGGGCAAGCCCTCAAAACCCAGCTCGTGGCATCTTCAACTTAGGCATGAGCAACGATACCAGTAAGCTTTCAGTTGGGCTCGGAAATAATGGAGAGAGTATAGAGATCAGTTTCTGGGATGGAAATAGCATCCGGTTTATATTTGAAAATTCGACTGCCAATATGTACAATTTTAGCTTCCACTCCAATGCAAATGAAGCATTCTACAGTTTCACTACCAGTAAGAATTACGACCTTGTGTGGTTTGTGATAAATTATACTGGAAACCTGGACCAGTATTATATGGTGGATGGGAAGATCTCGTCTGTGAGTCATGCTTTGTGTGCAGATTCAACCGGTGGTAATACTAGGACCTGCATTAATTCAATACCTTCTGACTGTGAAGATGGTGCTGACTTTTCTCAGATTAATGGTTTGCTGCCATCTACACCTAATACTAGTGGTTCCCATAATATGGTGATTCCTGATTGTGAAACTTTCTGCAAGAACAATTGTTCTTGTACTGCTTTCGCTTCAGTTCGGATTGGTCAACCAGGATGTCAATTTTACTATGGGAGTAAACAGGATTTATTGAAGAACATAGAGAAGGGGGCAGGGTTTATCTACATCCGAGGTGGTGGTCCAAGTGATGCTAAGAAGTGGAAAGTCAGGTTGGCTGTGGCGATTCCTTTGGCTTCTCTCTTGGTTTTTGTTCCAATCTCCTTCACCTGCTATCTGTGCTGGAGAAGGCAACTCAAAGGTCTCCTCCTAAACAAAGAATTAGGTGTACACAAGGATCAAGAATTGCCCCTTTATAGTTTTGCCACCATAAAGAAGGCAACAAATTACTTTTCAGAGTCTAATAAACTTGGAGAAGGTGGATATGGGCCTGTCTATAAGGGTGTATTGCTAGTTGATGGACAAGAAATTGCAGTGAAAAGGCTGTCCAAGATTTCCAGGCAAGGGTTGAGGGAGTTCAAAAACGAAATCTCACTGATTTGTAAGCTTCAACATAGAAATTTGGTTAGGCTTCTGGGATGCTGCATTGAAGCAGAAGAAAGTATACTGATTTATGAGTACTTGCCCAACAAAAGTCTAGATTTCTTCATTTTTGACTCAATCAAACATGTACTTTTGGATTGGAGAAGGCGTGTTAATATTATTGAAGGGATTGCACAAGGTCTTCTATACCTTCACAAATACTCGAGATTAAGGATCATTCACCGTGATTTAAAGACCAGCAATATTCTCCTGGATAGTGAGATGAACCCCAAGATTTCTGATTTTGGCATGGCAACAATTTTCGGGGACAGTGACATCAGAGGAAGAACAAACCGGGTTGTTGGTACATTTGGTTACATGTCTCCTGAGTATGCAACATATGGCCTCTTTTCTGAAAAATCTGATGTCTTTAGCTTTGGGGTGATCTTATTGGAGATCATAAGTGGCGAGAAGAACATTGCGTTCTTTGAGGCTGATCACACCCTGAACTTAATCAGCAAAGCTTGGCATTTGTGGAAAGAAGGGAGCAGCATGGAGTTAATGGATTCAACACTGCGTGCTTCGTGTTCAAGTGGTGAAGTTATCAGATGCATACATATAGGTCTTCTGTGTGTACAAGAAAGAGCTATTGATCGACCAACTATGTCGGATGTCATTTCTCTGCTAAGCAATCAGTCATCTGCATTGCCTCTACCTAAAGAACCTGCATTTTTGAGTCATTCAGGCTCCACTGATGCAGATTCATCTTCCAGTAGGCAAAGATATCACTCTCAAAATGAAGTGACCATATCAGAAGAATGCGGCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

782

Amino Acids

86.61

Weight (kDa)

5.86

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 87 - 185 1.5e-21 D-mannose binding lectin
PAN_2 PF08276 314 - 367 4e-06 PAN-like domain
Pkinase PF00069 463 - 672 3.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 465 - 732 9.3e-45 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 738 - 776 9.8e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000509)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29542 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29544 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29560
malus_domestica MD15G1121300.v1.1
prunus_persica Prupe.1G473900_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474100_v2.0.a1 Prupe.1G474300_v2.0.a1 Prupe.1G474500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298411 RchiOBHm_Chr6g0298421 RchiOBHm_Chr6g0298431 RchiOBHm_Chr6g0298441 RchiOBHm_Chr6g0298451 RchiOBHm_Chr6g0298461 RchiOBHm_Chr6g0298471 RchiOBHm_Chr6g0298481 RchiOBHm_Chr6g0298491 RchiOBHm_Chr6g0298501 RchiOBHm_Chr6g0298521
rosa_laevigata RLG00000011506 RLG00000011508 RLG00000011509 RLG00000011511 RLG00000011513 RLG00000011514 RLG00000011515
rosa_multiflora Rmu_co8314937.1_g000001 Rmu_co8319199.1_g000001 Rmu_sc0004500.1_g000002 Rmu_sc0006543.1_g000001 Rmu_ssc0000289.1_g000039 Rmu_ssc0000289.1_g000040 Rmu_ssc0000289.1_g000047 Rmu_ssc0000289.1_g000048 Rmu_ssc0000289.1_g000049 Rmu_ssc0000289.1_g000051
rosa_roxburghii Rroxscaffold_7G00169570 Rroxscaffold_7G00169580 Rroxscaffold_7G00169600 Rroxscaffold_7G00169610 Rroxscaffold_7G00169620 Rroxscaffold_7G00169630 Rroxscaffold_7G00169650 Rroxscaffold_7G00169660 Rroxscaffold_7G00169670
rosa_rugosa Rorug06G0280700 Rorug06G0280800 Rorug06G0280900
rosa_samantha Rh6AG391700 Rh6AG391800 Rh6AG392000 Rh6AG392100 Rh6AG392200 Rh6BG399300 Rh6BG399400 Rh6BG399500 Rh6BG399700 Rh6BG400000 Rh6CG405400 Rh6CG405500 Rh6CG405600 Rh6CG405700 Rh6CG405800 Rh6DG391800 Rh6DG391900 Rh6DG392000 Rh6DG392100 Rh6DG392300
rosa_wichuraiana Rw6G034230 Rw6G034240 Rw6G034250 Rw6G034260 Rw6G034270 Rw6G034280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1271
Acc36I ACCTGC 4 cut(s) 917, 1271, 2248, 2331
AccB7I CCANNNNNTGG 2 cut(s) 581, 1009
AccI GTMKAC 3 cut(s) 304, 346, 1597
AciI CCGC 1 cut(s) 2337
AclWI GGATC 3 cut(s) 242, 1338, 1757
AcsI RAATTY 4 cut(s) 325, 430, 703, 1552
AcuI CTGAAG 1 cut(s) 1053
AdeI CACNNNGTG 1 cut(s) 2093
AfaI GTAC 7 cut(s) 722, 1057, 1323, 1613, 1665, 1882, 2151
AfiI CCNNNNNNNGG 3 cut(s) 581, 1009, 1497
AflII CTTAAG 1 cut(s) 440
AflIII ACRYGT 2 cut(s) 1660, 1893
AgeI ACCGGT 1 cut(s) 890
AhlI ACTAGT 1 cut(s) 992
AjnI CCWGG 6 cut(s) 254, 505, 822, 1087, 1490, 1785
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
Alw26I GTCTC 2 cut(s) 1301, 1902
AlwI GGATC 3 cut(s) 242, 1338, 1757
AlwNI CAGNNNCTG 2 cut(s) 160, 669
Ama87I CYCGRG 1 cut(s) 1738
AoxI GGCC 3 cut(s) 387, 1421, 1918
ApeKI GCWGC 3 cut(s) 973, 1575, 2064
ApoI RAATTY 4 cut(s) 325, 430, 703, 1552
AseI ATTAAT 2 cut(s) 915, 963
AsiGI ACCGGT 1 cut(s) 890
AspS9I GGNCC 5 cut(s) 6, 826, 906, 1175, 1421
AsuC2I CCSGG 1 cut(s) 1871
AsuHPI GGTGA 6 cut(s) 41, 1024, 1252, 1748, 1969, 2123
AvaI CYCGRG 1 cut(s) 1738
AvaII GGWCC 4 cut(s) 6, 826, 906, 1175
BanII GRGCYC 1 cut(s) 636
BarI GAAGNNNNNNTAC 2 cut(s) 1581, 1613
BauI CACGAG 2 cut(s) 273, 579
BbsI GAAGAC 2 cut(s) 1709, 2132
BbvI GCAGC 3 cut(s) 960, 1562, 2076
BccI CCATC 5 cut(s) 668, 839, 935, 985, 1445
BciT130I CCWGG 6 cut(s) 256, 507, 824, 1089, 1492, 1787
BciVI GTATCC 1 cut(s) 445
BclI TGATCA 2 cut(s) 31, 2011
BcnI CCSGG 1 cut(s) 1871
BcoDI GTCTC 2 cut(s) 1301, 1902
BcuI ACTAGT 1 cut(s) 992
BfaI CTAG 5 cut(s) 90, 903, 993, 1445, 1631
BfmI CTRYAG 2 cut(s) 48, 760
BfrI CTTAAG 1 cut(s) 440
BfuAI ACCTGC 4 cut(s) 917, 1271, 2248, 2331
BfuI GTATCC 1 cut(s) 445
BglII AGATCT 1 cut(s) 852
BisI GCNGC 4 cut(s) 974, 1576, 2065, 2338
BlpI GCTNAGC 1 cut(s) 2201
BlsI GCNGC 4 cut(s) 975, 1577, 2066, 2339
BmcAI AGTACT 1 cut(s) 1613
Bme1390I CCNGG 7 cut(s) 256, 507, 824, 1089, 1492, 1787, 1871
Bme18I GGWCC 4 cut(s) 6, 826, 906, 1175
BmeT110I CYCGRG 1 cut(s) 1738
BmgT120I GGNCC 5 cut(s) 6, 826, 906, 1175, 1421
BmiI GGNNCC 2 cut(s) 1000, 2263
BmrFI CCNGG 7 cut(s) 256, 507, 824, 1089, 1492, 1787, 1871
BmsI GCATC 6 cut(s) 594, 693, 1174, 1562, 2114, 2260
BpiI GAAGAC 2 cut(s) 1709, 2132
BpmI CTGGAG 1 cut(s) 1297
Bpu1102I GCTNAGC 1 cut(s) 2201
BpuMI CCSGG 1 cut(s) 1871
BsaI GGTCTC 1 cut(s) 1301
BsaJI CCNNGG 3 cut(s) 9, 384, 1165
BsaWI WCCGGW 2 cut(s) 687, 890
BsaXI ACNNNNNCTCC 2 cut(s) 1669, 1699
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 1009, 1497
Bse118I RCCGGY 1 cut(s) 890
Bse1I ACTGG 7 cut(s) 294, 430, 615, 774, 820, 829, 2286
Bse3DI GCAATG 2 cut(s) 1992, 2219
BseBI CCWGG 6 cut(s) 256, 507, 824, 1089, 1492, 1787
BseDI CCNNGG 3 cut(s) 9, 384, 1165
BseGI GGATG 7 cut(s) 679, 684, 850, 1097, 1161, 1577, 2191
BseLI CCNNNNNNNGG 3 cut(s) 581, 1009, 1497
BseMI GCAATG 2 cut(s) 1992, 2219
BseMII CTCAG 3 cut(s) 384, 971, 1893
BseNI ACTGG 7 cut(s) 294, 430, 615, 774, 820, 829, 2286
BseRI GAGGAG 1 cut(s) 1289
BseXI GCAGC 3 cut(s) 960, 1562, 2076
BseYI CCCAGC 1 cut(s) 574
BsgI GTGCAG 1 cut(s) 900
BshFI GGCC 3 cut(s) 389, 1423, 1920
BshTI ACCGGT 1 cut(s) 890
BsiHKCI CYCGRG 1 cut(s) 1738
BsiSI CCGG 3 cut(s) 688, 891, 1870
BslFI GGGAC 2 cut(s) 161, 1856
BslI CCNNNNNNNGG 3 cut(s) 581, 1009, 1497
BsmAI GTCTC 2 cut(s) 1301, 1902
BsmFI GGGAC 2 cut(s) 161, 1856
BsmI GAATGC 2 cut(s) 755, 2339
BsnI GGCC 3 cut(s) 389, 1423, 1920
Bso31I GGTCTC 1 cut(s) 1301
BsoBI CYCGRG 1 cut(s) 1738
Bsp1286I GDGCHC 1 cut(s) 636
Bsp1407I TGTACA 3 cut(s) 720, 1321, 2149
Bsp1720I GCTNAGC 1 cut(s) 2201
BspACI CCGC 1 cut(s) 2337
BspANI GGCC 3 cut(s) 389, 1423, 1920
BspCNI CTCAG 3 cut(s) 383, 970, 1894
BspLI GGNNCC 2 cut(s) 1000, 2263
BspMAI CTGCAG 1 cut(s) 52
BspMI ACCTGC 4 cut(s) 917, 1271, 2248, 2331
BspPI GGATC 3 cut(s) 242, 1338, 1757
BspTI CTTAAG 1 cut(s) 440
BspTNI GGTCTC 1 cut(s) 1301
BsrDI GCAATG 2 cut(s) 1992, 2219
BsrFI RCCGGY 1 cut(s) 890
BsrGI TGTACA 3 cut(s) 720, 1321, 2149
BsrI ACTGG 7 cut(s) 294, 430, 615, 774, 820, 829, 2286
BssAI RCCGGY 1 cut(s) 890
BssECI CCNNGG 3 cut(s) 9, 384, 1165
BssNAI GTATAC 1 cut(s) 1598
BssSI CACGAG 2 cut(s) 273, 579
BssT1I CCWWGG 2 cut(s) 9, 384
Bst1107I GTATAC 1 cut(s) 1598
Bst2BI CACGAG 2 cut(s) 273, 579
Bst2UI CCWGG 6 cut(s) 256, 507, 824, 1089, 1492, 1787
Bst4CI ACNGT 6 cut(s) 132, 344, 764, 935, 1760, 1847
BstAFI CTTAAG 1 cut(s) 440
BstAUI TGTACA 3 cut(s) 720, 1321, 2149
BstC8I GCNNGC 2 cut(s) 562, 2094
BstDEI CTNAG 6 cut(s) 370, 595, 957, 1188, 1902, 2201
BstF5I GGATG 7 cut(s) 679, 684, 850, 1097, 1161, 1577, 2191
BstMAI GTCTC 2 cut(s) 1301, 1902
BstMWI GCNNNNNNNGC 5 cut(s) 213, 395, 752, 878, 1272
BstNI CCWGG 6 cut(s) 256, 507, 824, 1089, 1492, 1787
BstNSI RCATGY 2 cut(s) 1664, 1897
BstSCI CCNGG 7 cut(s) 254, 505, 822, 1087, 1490, 1785, 1869
BstSFI CTRYAG 2 cut(s) 48, 760
BstV1I GCAGC 3 cut(s) 960, 1562, 2076
BstV2I GAAGAC 2 cut(s) 1709, 2132
BstX2I RGATCY 1 cut(s) 852
BstYI RGATCY 1 cut(s) 852
BstZ17I GTATAC 1 cut(s) 1598
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 3 cut(s) 389, 1423, 1920
BtsCI GGATG 7 cut(s) 679, 684, 850, 1097, 1161, 1577, 2191
BtsI GCAGTG 2 cut(s) 1473, 2087
BtsIMutI CAGTG 5 cut(s) 1473, 1526, 1852, 2087, 2265
BveI ACCTGC 4 cut(s) 917, 1271, 2248, 2331
Cac8I GCNNGC 2 cut(s) 562, 2094
CaiI CAGNNNCTG 2 cut(s) 160, 669
Cfr10I RCCGGY 1 cut(s) 890
Cfr13I GGNCC 5 cut(s) 6, 826, 906, 1175, 1421
Csp6I GTAC 7 cut(s) 721, 1056, 1322, 1612, 1664, 1881, 2150
CspAI ACCGGT 1 cut(s) 890
CviAII CATG 7 cut(s) 55, 601, 869, 1661, 1825, 1894, 2068
CviQI GTAC 7 cut(s) 721, 1056, 1322, 1612, 1664, 1881, 2150
DdeI CTNAG 6 cut(s) 370, 595, 957, 1188, 1902, 2201
DraI TTTAAA 1 cut(s) 1767
DraIII CACNNNGTG 1 cut(s) 2093
Eco130I CCWWGG 2 cut(s) 9, 384
Eco24I GRGCYC 1 cut(s) 636
Eco31I GGTCTC 1 cut(s) 1301
Eco32I GATATC 2 cut(s) 81, 2300
Eco47I GGWCC 4 cut(s) 6, 826, 906, 1175
Eco57I CTGAAG 1 cut(s) 1053
Eco88I CYCGRG 1 cut(s) 1738
EcoO109I RGGNCCY 1 cut(s) 906
EcoRII CCWGG 6 cut(s) 254, 505, 822, 1087, 1490, 1785
EcoRV GATATC 2 cut(s) 81, 2300
EcoT14I CCWWGG 2 cut(s) 9, 384
EcoT22I ATGCAT 1 cut(s) 2129
EcoT38I GRGCYC 1 cut(s) 636
ErhI CCWWGG 2 cut(s) 9, 384
FaeI CATG 7 cut(s) 58, 604, 872, 1664, 1828, 1897, 2071
FaqI GGGAC 2 cut(s) 161, 1856
FatI CATG 7 cut(s) 54, 600, 868, 1660, 1824, 1893, 2067
FauNDI CATATG 1 cut(s) 1915
FbaI TGATCA 2 cut(s) 31, 2011
FblI GTMKAC 3 cut(s) 304, 346, 1597
Fnu4HI GCNGC 4 cut(s) 974, 1576, 2065, 2338
FokI GGATG 7 cut(s) 671, 686, 857, 1104, 1148, 1584, 2198
FriOI GRGCYC 1 cut(s) 636
Fsp4HI GCNGC 4 cut(s) 974, 1576, 2065, 2338
FspBI CTAG 5 cut(s) 90, 903, 993, 1445, 1631
GluI GCNGC 4 cut(s) 974, 1576, 2065, 2338
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 1 cut(s) 1297
HaeIII GGCC 3 cut(s) 389, 1423, 1920
HapII CCGG 3 cut(s) 688, 891, 1870
Hin1II CATG 7 cut(s) 58, 604, 872, 1664, 1828, 1897, 2071
HincII GTYRAC 2 cut(s) 347, 1085
HindII GTYRAC 2 cut(s) 347, 1085
HindIII AAGCTT 4 cut(s) 515, 620, 1538, 2037
HpaII CCGG 3 cut(s) 688, 891, 1870
HphI GGTGA 6 cut(s) 41, 1024, 1252, 1748, 1969, 2123
Hpy166II GTNNAC 8 cut(s) 305, 347, 1085, 1115, 1322, 1324, 1598, 2150
Hpy188III TCNNGA 8 cut(s) 275, 372, 419, 1019, 1334, 1631, 1740, 1901
Hpy8I GTNNAC 8 cut(s) 305, 347, 1085, 1115, 1322, 1324, 1598, 2150
Hpy99I CGWCG 1 cut(s) 348
HpyCH4III ACNGT 6 cut(s) 132, 344, 764, 935, 1760, 1847
HpyF10VI GCNNNNNNNGC 5 cut(s) 213, 395, 752, 878, 1272
HpyF3I CTNAG 6 cut(s) 370, 595, 957, 1188, 1902, 2201
Hsp92II CATG 7 cut(s) 58, 604, 872, 1664, 1828, 1897, 2071
Ksp22I TGATCA 2 cut(s) 31, 2011
LmnI GCTCC 3 cut(s) 13, 2061, 2267
Lsp1109I GCAGC 3 cut(s) 960, 1562, 2076
LweI GCATC 6 cut(s) 594, 693, 1174, 1562, 2114, 2260
MaeI CTAG 5 cut(s) 90, 903, 993, 1445, 1631
MaeIII GTNAC 3 cut(s) 1847, 1889, 2317
MfeI CAATTG 2 cut(s) 356, 1045
MflI RGATCY 1 cut(s) 852
MhlI GDGCHC 1 cut(s) 636
MlyI GAGTC 5 cut(s) 131, 874, 1400, 1643, 2260
MmeI TCCRAC 2 cut(s) 275, 2163
MnlI CCTC 9 cut(s) 138, 576, 1160, 1310, 1497, 1850, 1931, 1999, 2235
Mph1103I ATGCAT 1 cut(s) 2129
MseI TTAA 9 cut(s) 68, 441, 915, 963, 1689, 1745, 1766, 2027, 2075
MslI CAYNNNNRTG 1 cut(s) 741
MspA1I CMGCKG 1 cut(s) 170
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 3 cut(s) 688, 891, 1870
MspR9I CCNGG 7 cut(s) 256, 507, 824, 1089, 1492, 1787, 1871
MunI CAATTG 2 cut(s) 356, 1045
Mva1269I GAATGC 2 cut(s) 755, 2339
MvaI CCWGG 6 cut(s) 256, 507, 824, 1089, 1492, 1787
MwoI GCNNNNNNNGC 5 cut(s) 213, 395, 752, 878, 1272
NciI CCSGG 1 cut(s) 1871
NdeI CATATG 1 cut(s) 1915
NlaIII CATG 7 cut(s) 58, 604, 872, 1664, 1828, 1897, 2071
NlaIV GGNNCC 2 cut(s) 1000, 2263
NmuCI GTSAC 2 cut(s) 1847, 2317
NsiI ATGCAT 1 cut(s) 2129
NspI RCATGY 2 cut(s) 1664, 1897
PaeR7I CTCGAG 1 cut(s) 1738
PaqCI CACCTGC 1 cut(s) 1271
PciI ACATGT 2 cut(s) 1660, 1893
PctI GAATGC 2 cut(s) 755, 2339
PfeI GAWTC 7 cut(s) 20, 293, 884, 1015, 1219, 2081, 2276
PflMI CCANNNNNTGG 2 cut(s) 581, 1009
PfoI TCCNGGA 1 cut(s) 1785
PinAI ACCGGT 1 cut(s) 890
PkrI GCNGC 4 cut(s) 975, 1577, 2066, 2339
PleI GAGTC 5 cut(s) 131, 873, 1399, 1643, 2259
PpsI GAGTC 5 cut(s) 131, 873, 1399, 1643, 2259
PpuMI RGGWCCY 1 cut(s) 906
PscI ACATGT 2 cut(s) 1660, 1893
PshBI ATTAAT 2 cut(s) 915, 963
Psp5II RGGWCCY 1 cut(s) 906
Psp6I CCWGG 6 cut(s) 254, 505, 822, 1087, 1490, 1785
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 6 cut(s) 254, 505, 822, 1087, 1490, 1785
PspN4I GGNNCC 2 cut(s) 1000, 2263
PspPI GGNCC 5 cut(s) 6, 826, 906, 1175, 1421
PspPPI RGGWCCY 1 cut(s) 906
PstI CTGCAG 1 cut(s) 52
PstNI CAGNNNCTG 2 cut(s) 160, 669
PsuI RGATCY 1 cut(s) 852
PvuII CAGCTG 1 cut(s) 170
RsaI GTAC 7 cut(s) 722, 1057, 1323, 1613, 1665, 1882, 2151
RsaNI GTAC 7 cut(s) 721, 1056, 1322, 1612, 1664, 1881, 2150
RseI CAYNNNNRTG 1 cut(s) 741
SalI GTCGAC 1 cut(s) 345
SaqAI TTAA 9 cut(s) 68, 441, 915, 963, 1689, 1745, 1766, 2027, 2075
SatI GCNGC 4 cut(s) 974, 1576, 2065, 2338
Sau96I GGNCC 5 cut(s) 6, 826, 906, 1175, 1421
ScaI AGTACT 1 cut(s) 1613
SchI GAGTC 5 cut(s) 131, 874, 1400, 1643, 2260
ScrFI CCNGG 7 cut(s) 256, 507, 824, 1089, 1492, 1787, 1871
SduI GDGCHC 1 cut(s) 636
SfaNI GCATC 6 cut(s) 594, 693, 1174, 1562, 2114, 2260
SfcI CTRYAG 2 cut(s) 48, 760
Sfr274I CTCGAG 1 cut(s) 1738
SinI GGWCC 4 cut(s) 6, 826, 906, 1175
SlaI CTCGAG 1 cut(s) 1738
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 2 cut(s) 440, 1738
SmoI CTYRAG 2 cut(s) 440, 1738
SpeI ACTAGT 1 cut(s) 992
SsiI CCGC 1 cut(s) 2337
SspI AATATT 3 cut(s) 547, 1693, 1780
SspMI CTAG 5 cut(s) 90, 903, 993, 1445, 1631
StyD4I CCNGG 7 cut(s) 254, 505, 822, 1087, 1490, 1785, 1869
StyI CCWWGG 2 cut(s) 9, 384
TaaI ACNGT 6 cut(s) 132, 344, 764, 935, 1760, 1847
TaqI TCGA 4 cut(s) 346, 707, 1739, 2170
TatI WGTACW 6 cut(s) 720, 1055, 1321, 1611, 1663, 2149
TauI GCSGC 1 cut(s) 2340
TfiI GAWTC 7 cut(s) 20, 293, 884, 1015, 1219, 2081, 2276
Tru1I TTAA 9 cut(s) 68, 441, 915, 963, 1689, 1745, 1766, 2027, 2075
Tru9I TTAA 9 cut(s) 68, 441, 915, 963, 1689, 1745, 1766, 2027, 2075
TscAI CASTG 5 cut(s) 1473, 1533, 1852, 2094, 2272
TseFI GTSAC 2 cut(s) 1847, 2317
TseI GCWGC 3 cut(s) 973, 1575, 2064
Tsp45I GTSAC 2 cut(s) 1847, 2317
TspRI CASTG 5 cut(s) 1473, 1533, 1852, 2094, 2272
Van91I CCANNNNNTGG 2 cut(s) 581, 1009
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 4 cut(s) 6, 826, 906, 1175
VspI ATTAAT 2 cut(s) 915, 963
XapI RAATTY 4 cut(s) 325, 430, 703, 1552
XbaI TCTAGA 1 cut(s) 1630
XceI RCATGY 2 cut(s) 1664, 1897
XhoI CTCGAG 1 cut(s) 1738
XmiI GTMKAC 3 cut(s) 304, 346, 1597
XspI CTAG 5 cut(s) 90, 903, 993, 1445, 1631
ZrmI AGTACT 1 cut(s) 1613
Zsp2I ATGCAT 1 cut(s) 2129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.