Rroxscaffold_7G00169630

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000509
Physical Location & Seq
Forward (+)
10220125 .. 10223369
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00169630.1

Sequence Viewer

Length: 2025 bp
ATGGCCATGCTTCTTCCTCTAATTTGCTTGATATCATCATTCTTTAGCTTCCCTTCGTTTTCATCTCCTGCAACAGATACTCTTGCACCTTCAGACACGTTGAGAGATGGTCAAATCCTGGTTTCAGCTGGTGGAGTTTTTGAGCTGGGCTTGTTCAATGAAAGCTTTTCAGGCTATCACTATCTGGGAATTTGGTTGAGAGCCGATGCAACCAAGGTTGTCTGGGTTGATCGCGATACTCCCATATTGGATTCATCAGGCATGCTTAAAATCCTGTCTGGGAATTTGGTTCTCACAGACCGTCGACAAGTGCAATTGATAGTCAACACAGGAAGTCTTGCCACGGCCACAACTGCCAACACAAGTGCAACACTTCTTGATACTGGAAATTTTGTCCTCAAGGAAGCAGATACAGGTAATACCATATGGCAAAGTTTTGATGTACCGAGTGATACTTATCTTCCTGGGATGAAACTTGGGTTGTTTGGCCGAAACCTATTTCACAGTATTGTTTCTTGGGCAAGCCCCCAAAACCCAGCTCGTGGCATCTTCACCTTAAGCATTAATAGCAACGACACCAGAAAGCTTTCAGTTCAGCGAGGAAATAATGGAGAGAAAATAGATATCAGTTTCTGGGATGGAAATGGCATCCGGTTTATATTTGAAAATTCAACTGCCACTATGTACAATTTTAGCTTCCAGTCCAATGCAACCGAAGCATTCTACACTTTTACTACCAGTAAGAATTATGACCTTGTGTGGTTTGTGATGAATTACACTGGAAACCTGGACCAATATTTTATGGTGGATGGGAAGATCTCGTCTGTGAGTCATGCTTTGTGTGAGGAGTCAGCCGGTGGTAGTACTCGGACGTGTTTTAGTACAATACCTTCTAACTGTGAAGATGGTGCTGACTTTTCTCAGATTAATGGTTTGCTGCCATCTACATCTAATACTACAGGTTCCCTAAATATGGTGATTACTGATTGTGAAAATTTCTGCAAGAACAATTGTTCTTGTACCGCATTTGCTTCAGTTCAGAATGGTCAACCAGGATGTCAACTTTACTATGGGAGTAAACAGGATTTATTGAAGAACATAGAGAAGGGGGCAGGGCTTATTTACATCAGGGGTGGTGCTCCAAGTGTTGAAGGACATGAAATTGCAGTGAAAAGGCTGTCCAAGATTTCGAGGCAAGGGTTGAGGGAGTTCAAAAACGAAGTCTCACTGATTTGTAAGCTTCAACATAGAAACTTGGTTAGGCTTCTGGGATGCTGCATTGAAGCAGAAGAAAGCATACTGATTTACGAGTACATGCCCAACAAAAACTCAACCAAACAGGTACTTTTGGATTGGAGAAGGCGTGTTAACATTATTGAAGGGATTGCACAAGGTCTTCTGTATCTTCACAAATACTCGAGATTAAGGATCATTCACCGTGATTTAAAGACCAGCAATATTCTGCTGGACAGTGACATGAACCCCAAGATTTCTGATTTTGGCATGGCACGAATTTTTAGGGACAATGACATCAGAGGAAAAACAAACCGGGTTGTTGGTACATTTGGTTATATGTCTCCTGAGTACGCAATGGGGGGTCTCTTTTCTGAAAAATCCGATGTCTTTAGCTTTGGGGTCATCTTATTAGAGATCATAAGTGGCGAGAAGAATATTGCATTCTTTGAGGCTGATCACTCTCTGAACTTACTCAGCAAAGCTTGGCATTTGTGGAAAGAAGGAATCAGCATGGAGTTAATGGATTCAACACTGCGTGCTTCGTGTTCAAGCAGTGAAGTTATCAGATGCATACAGATGGGTCTTCTGTGCGTACAAGAAAAAGCTAATGATCGACCAACCATGTCGGATGTTGTTTCTATGCTAAGCAATCAGCCATTTGCTTTGCCTTTTCCCAAAGAACCTGCATTTTTGAGTCATTCAAGCTCCAGTGATGCAGATTCATCTTCCATTAGGCAAAGATATTACTCTCAAAATGATATGACAATTTCAGAAGAATGTGGCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

674

Amino Acids

74.8

Weight (kDa)

5.8

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 173 2.2e-23 D-mannose binding lectin
PAN_2 PF08276 302 - 356 1.6e-06 PAN-like domain
PK_Tyr_Ser-Thr PF07714 384 - 627 1.1e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 384 - 625 2.9e-37 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000509)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29542 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29544 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29560
malus_domestica MD15G1121300.v1.1
prunus_persica Prupe.1G473900_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474100_v2.0.a1 Prupe.1G474300_v2.0.a1 Prupe.1G474500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298411 RchiOBHm_Chr6g0298421 RchiOBHm_Chr6g0298431 RchiOBHm_Chr6g0298441 RchiOBHm_Chr6g0298451 RchiOBHm_Chr6g0298461 RchiOBHm_Chr6g0298471 RchiOBHm_Chr6g0298481 RchiOBHm_Chr6g0298491 RchiOBHm_Chr6g0298501 RchiOBHm_Chr6g0298521
rosa_laevigata RLG00000011506 RLG00000011508 RLG00000011509 RLG00000011511 RLG00000011513 RLG00000011514 RLG00000011515
rosa_multiflora Rmu_co8314937.1_g000001 Rmu_co8319199.1_g000001 Rmu_sc0004500.1_g000002 Rmu_sc0006543.1_g000001 Rmu_ssc0000289.1_g000039 Rmu_ssc0000289.1_g000040 Rmu_ssc0000289.1_g000047 Rmu_ssc0000289.1_g000048 Rmu_ssc0000289.1_g000049 Rmu_ssc0000289.1_g000051
rosa_roxburghii Rroxscaffold_7G00169570 Rroxscaffold_7G00169580 Rroxscaffold_7G00169600 Rroxscaffold_7G00169610 Rroxscaffold_7G00169620 Rroxscaffold_7G00169630 Rroxscaffold_7G00169650 Rroxscaffold_7G00169660 Rroxscaffold_7G00169670
rosa_rugosa Rorug06G0280700 Rorug06G0280800 Rorug06G0280900
rosa_samantha Rh6AG391700 Rh6AG391800 Rh6AG392000 Rh6AG392100 Rh6AG392200 Rh6BG399300 Rh6BG399400 Rh6BG399500 Rh6BG399700 Rh6BG400000 Rh6CG405400 Rh6CG405500 Rh6CG405600 Rh6CG405700 Rh6CG405800 Rh6DG391800 Rh6DG391900 Rh6DG392000 Rh6DG392100 Rh6DG392300
rosa_wichuraiana Rw6G034230 Rw6G034240 Rw6G034250 Rw6G034260 Rw6G034270 Rw6G034280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1927, 2010
AccB7I CCANNNNNTGG 1 cut(s) 542
AccI GTMKAC 1 cut(s) 304
AccII CGCG 1 cut(s) 234
AciI CCGC 1 cut(s) 1023
AclWI GGATC 1 cut(s) 1436
AcoI YGGCCR 3 cut(s) 3, 345, 487
AcsI RAATTY 6 cut(s) 189, 283, 388, 667, 994, 1512
AcuI CTGAAG 2 cut(s) 75, 1017
AdeI CACNNNGTG 1 cut(s) 1772
AfiI CCNNNNNNNGG 2 cut(s) 542, 973
AflII CTTAAG 1 cut(s) 556
AflIII ACRYGT 2 cut(s) 96, 872
AjiI CACGTC 1 cut(s) 873
AjnI CCWGG 4 cut(s) 117, 463, 786, 1051
Alw21I GWGCWC 1 cut(s) 1141
Alw26I GTCTC 3 cut(s) 1228, 1581, 1604
AlwI GGATC 1 cut(s) 1436
AlwNI CAGNNNCTG 1 cut(s) 633
Ama87I CYCGRG 1 cut(s) 1417
AoxI GGCC 3 cut(s) 3, 345, 487
ApeKI GCWGC 2 cut(s) 937, 1275
ApoI RAATTY 6 cut(s) 189, 283, 388, 667, 994, 1512
ArsI GACNNNNNNTTYG 2 cut(s) 1206, 1238
AseI ATTAAT 2 cut(s) 564, 927
Asp700I GAANNNNTTC 1 cut(s) 586
AspS9I GGNCC 1 cut(s) 790
AsuC2I CCSGG 1 cut(s) 1550
AsuHPI GGTGA 3 cut(s) 544, 988, 1427
AvaI CYCGRG 1 cut(s) 1417
AvaII GGWCC 1 cut(s) 790
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 4 cut(s) 874, 906, 1281, 1313
BauI CACGAG 1 cut(s) 540
BbsI GAAGAC 2 cut(s) 1388, 1811
Bbv12I GWGCWC 1 cut(s) 1141
BbvI GCAGC 2 cut(s) 924, 1262
BccI CCATC 6 cut(s) 101, 632, 803, 899, 949, 1807
BceAI ACGGC 1 cut(s) 360
BciT130I CCWGG 4 cut(s) 119, 465, 788, 1053
BclI TGATCA 1 cut(s) 1690
BcnI CCSGG 1 cut(s) 1550
BcoDI GTCTC 3 cut(s) 1228, 1581, 1604
BfmI CTRYAG 1 cut(s) 957
BfrI CTTAAG 1 cut(s) 556
BfuAI ACCTGC 2 cut(s) 1927, 2010
BglII AGATCT 1 cut(s) 816
BisI GCNGC 2 cut(s) 938, 1276
BlpI GCTNAGC 1 cut(s) 1880
BlsI GCNGC 2 cut(s) 939, 1277
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 5 cut(s) 119, 465, 788, 1053, 1550
Bme18I GGWCC 1 cut(s) 790
BmeT110I CYCGRG 1 cut(s) 1417
BmgBI CACGTC 1 cut(s) 873
BmgT120I GGNCC 1 cut(s) 790
BmiI GGNNCC 1 cut(s) 964
BmrFI CCNGG 5 cut(s) 119, 465, 788, 1053, 1550
BmsI GCATC 6 cut(s) 196, 555, 657, 1262, 1793, 1939
BpiI GAAGAC 2 cut(s) 1388, 1811
BpmI CTGGAG 1 cut(s) 1927
Bpu1102I GCTNAGC 1 cut(s) 1880
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 1550
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 1604
BsaJI CCNNGG 3 cut(s) 213, 342, 464
BsaWI WCCGGW 1 cut(s) 651
BsaXI ACNNNNNCTCC 2 cut(s) 1348, 1378
Bsc4I CCNNNNNNNGG 2 cut(s) 542, 973
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 5 cut(s) 388, 700, 738, 784, 1944
Bse3DI GCAATG 1 cut(s) 1596
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 4 cut(s) 119, 465, 788, 1053
BseDI CCNNGG 3 cut(s) 213, 342, 464
BseGI GGATG 7 cut(s) 474, 643, 648, 814, 1061, 1277, 1870
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 2 cut(s) 542, 973
BseMI GCAATG 1 cut(s) 1596
BseMII CTCAG 3 cut(s) 935, 1572, 1723
BseNI ACTGG 5 cut(s) 388, 700, 738, 784, 1944
BseRI GAGGAG 1 cut(s) 860
BseXI GCAGC 2 cut(s) 924, 1262
BseYI CCCAGC 2 cut(s) 145, 535
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 3 cut(s) 5, 347, 489
BsiHKAI GWGCWC 1 cut(s) 1141
BsiHKCI CYCGRG 1 cut(s) 1417
BsiSI CCGG 3 cut(s) 652, 855, 1549
BslFI GGGAC 1 cut(s) 1535
BslI CCNNNNNNNGG 2 cut(s) 542, 973
BsmAI GTCTC 3 cut(s) 1228, 1581, 1604
BsmFI GGGAC 1 cut(s) 1535
BsmI GAATGC 2 cut(s) 719, 1676
BsnI GGCC 3 cut(s) 5, 347, 489
Bso31I GGTCTC 1 cut(s) 1604
BsoBI CYCGRG 1 cut(s) 1417
Bsp1286I GDGCHC 1 cut(s) 1141
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 6 cut(s) 229, 816, 1428, 1650, 1690, 1846
Bsp1720I GCTNAGC 1 cut(s) 1880
Bsp68I TCGCGA 1 cut(s) 234
BspACI CCGC 1 cut(s) 1023
BspANI GGCC 3 cut(s) 5, 347, 489
BspCNI CTCAG 3 cut(s) 934, 1573, 1722
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 964
BspMI ACCTGC 2 cut(s) 1927, 2010
BspPI GGATC 1 cut(s) 1436
BspTI CTTAAG 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 1604
BsrDI GCAATG 1 cut(s) 1596
BsrFI RCCGGY 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 5 cut(s) 388, 700, 738, 784, 1944
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 3 cut(s) 213, 342, 464
BssMI GATC 6 cut(s) 229, 816, 1428, 1650, 1690, 1846
BssSI CACGAG 1 cut(s) 540
BssT1I CCWWGG 1 cut(s) 213
Bst2BI CACGAG 1 cut(s) 540
Bst2UI CCWGG 4 cut(s) 119, 465, 788, 1053
Bst4CI ACNGT 5 cut(s) 302, 506, 899, 1439, 1472
BstAFI CTTAAG 1 cut(s) 556
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 3 cut(s) 263, 523, 1773
BstDEI CTNAG 4 cut(s) 921, 1581, 1709, 1880
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 7 cut(s) 474, 643, 648, 814, 1061, 1277, 1870
BstFNI CGCG 1 cut(s) 234
BstKTI GATC 6 cut(s) 232, 819, 1431, 1653, 1693, 1849
BstMAI GTCTC 3 cut(s) 1228, 1581, 1604
BstMBI GATC 6 cut(s) 229, 816, 1428, 1650, 1690, 1846
BstMWI GCNNNNNNNGC 4 cut(s) 171, 353, 567, 716
BstNI CCWGG 4 cut(s) 119, 465, 788, 1053
BstNSI RCATGY 2 cut(s) 265, 1318
BstSCI CCNGG 5 cut(s) 117, 463, 786, 1051, 1548
BstSFI CTRYAG 1 cut(s) 957
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 2 cut(s) 924, 1262
BstV2I GAAGAC 2 cut(s) 1388, 1811
BstX2I RGATCY 1 cut(s) 816
BstYI RGATCY 1 cut(s) 816
BsuRI GGCC 3 cut(s) 5, 347, 489
BtgI CCRYGG 1 cut(s) 342
BtrI CACGTC 1 cut(s) 873
BtsCI GGATG 7 cut(s) 474, 643, 648, 814, 1061, 1277, 1870
BtsI GCAGTG 3 cut(s) 1173, 1766, 1795
BtsIMutI CAGTG 7 cut(s) 777, 1173, 1226, 1477, 1766, 1795, 1951
BtuMI TCGCGA 1 cut(s) 234
BveI ACCTGC 2 cut(s) 1927, 2010
Cac8I GCNNGC 3 cut(s) 263, 523, 1773
CaiI CAGNNNCTG 1 cut(s) 633
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 1 cut(s) 790
CviAII CATG 9 cut(s) 7, 262, 833, 1157, 1315, 1477, 1504, 1747, 1858
DdeI CTNAG 4 cut(s) 921, 1581, 1709, 1880
DpnI GATC 6 cut(s) 231, 818, 1430, 1652, 1692, 1848
DpnII GATC 6 cut(s) 229, 816, 1428, 1650, 1690, 1846
DraI TTTAAA 1 cut(s) 1446
DraIII CACNNNGTG 1 cut(s) 1772
EaeI YGGCCR 3 cut(s) 3, 345, 487
Eco130I CCWWGG 1 cut(s) 213
Eco31I GGTCTC 1 cut(s) 1604
Eco32I GATATC 2 cut(s) 33, 625
Eco47I GGWCC 1 cut(s) 790
Eco57I CTGAAG 2 cut(s) 75, 1017
Eco88I CYCGRG 1 cut(s) 1417
EcoRII CCWGG 4 cut(s) 117, 463, 786, 1051
EcoRV GATATC 2 cut(s) 33, 625
EcoT14I CCWWGG 1 cut(s) 213
EcoT22I ATGCAT 1 cut(s) 1808
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 9 cut(s) 10, 265, 836, 1160, 1318, 1480, 1507, 1750, 1861
FaqI GGGAC 1 cut(s) 1535
FatI CATG 9 cut(s) 6, 261, 832, 1156, 1314, 1476, 1503, 1746, 1857
FauNDI CATATG 1 cut(s) 425
FbaI TGATCA 1 cut(s) 1690
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 2 cut(s) 938, 1276
FokI GGATG 7 cut(s) 481, 635, 650, 821, 1068, 1284, 1877
Fsp4HI GCNGC 2 cut(s) 938, 1276
GluI GCNGC 2 cut(s) 938, 1276
GsaI CCCAGC 2 cut(s) 149, 539
GsuI CTGGAG 1 cut(s) 1927
HaeIII GGCC 3 cut(s) 5, 347, 489
HapII CCGG 3 cut(s) 652, 855, 1549
Hin1II CATG 9 cut(s) 10, 265, 836, 1160, 1318, 1480, 1507, 1750, 1861
HincII GTYRAC 5 cut(s) 305, 325, 1049, 1061, 1369
HindII GTYRAC 5 cut(s) 305, 325, 1049, 1061, 1369
HindIII AAGCTT 4 cut(s) 163, 584, 1238, 1716
HinfI GANTC 7 cut(s) 251, 829, 848, 1740, 1760, 1930, 1955
HpaI GTTAAC 1 cut(s) 1369
HpaII CCGG 3 cut(s) 652, 855, 1549
HphI GGTGA 3 cut(s) 544, 988, 1427
Hpy166II GTNNAC 6 cut(s) 305, 325, 1049, 1061, 1079, 1369
Hpy188III TCNNGA 4 cut(s) 233, 377, 1419, 1580
Hpy8I GTNNAC 6 cut(s) 305, 325, 1049, 1061, 1079, 1369
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 8 cut(s) 63, 99, 900, 1099, 1145, 1353, 1373, 1730
HpyCH4III ACNGT 5 cut(s) 302, 506, 899, 1439, 1472
HpyCH4IV ACGT 2 cut(s) 98, 872
HpyF10VI GCNNNNNNNGC 4 cut(s) 171, 353, 567, 716
HpyF3I CTNAG 4 cut(s) 921, 1581, 1709, 1880
HpySE526I ACGT 2 cut(s) 98, 872
Hsp92II CATG 9 cut(s) 10, 265, 836, 1160, 1318, 1480, 1507, 1750, 1861
Ksp22I TGATCA 1 cut(s) 1690
KspAI GTTAAC 1 cut(s) 1369
Kzo9I GATC 6 cut(s) 229, 816, 1428, 1650, 1690, 1846
LmnI GCTCC 2 cut(s) 1144, 1946
Lsp1109I GCAGC 2 cut(s) 924, 1262
LweI GCATC 6 cut(s) 196, 555, 657, 1262, 1793, 1939
MaeII ACGT 2 cut(s) 98, 872
MaeIII GTNAC 1 cut(s) 1472
MalI GATC 6 cut(s) 231, 818, 1430, 1652, 1692, 1848
MboI GATC 6 cut(s) 229, 816, 1428, 1650, 1690, 1846
MfeI CAATTG 2 cut(s) 314, 1009
MflI RGATCY 1 cut(s) 816
MhlI GDGCHC 1 cut(s) 1141
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 838, 857, 1939
MmeI TCCRAC 1 cut(s) 1842
MnlI CCTC 8 cut(s) 27, 407, 593, 838, 1185, 1197, 1529, 1678
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 1808
MroXI GAANNNNTTC 1 cut(s) 586
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 267, 557, 564, 927, 1368, 1424, 1445, 1754
MslI CAYNNNNRTG 1 cut(s) 1811
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 128
MspCI CTTAAG 1 cut(s) 556
MspI CCGG 3 cut(s) 652, 855, 1549
MspR9I CCNGG 5 cut(s) 119, 465, 788, 1053, 1550
MunI CAATTG 2 cut(s) 314, 1009
Mva1269I GAATGC 2 cut(s) 719, 1676
MvaI CCWGG 4 cut(s) 119, 465, 788, 1053
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 4 cut(s) 171, 353, 567, 716
NciI CCSGG 1 cut(s) 1550
NdeI CATATG 1 cut(s) 425
NdeII GATC 6 cut(s) 229, 816, 1428, 1650, 1690, 1846
NlaIII CATG 9 cut(s) 10, 265, 836, 1160, 1318, 1480, 1507, 1750, 1861
NlaIV GGNNCC 1 cut(s) 964
NmuCI GTSAC 1 cut(s) 1472
NruI TCGCGA 1 cut(s) 234
NsiI ATGCAT 1 cut(s) 1808
NspI RCATGY 2 cut(s) 265, 1318
PaeI GCATGC 1 cut(s) 265
PaeR7I CTCGAG 1 cut(s) 1417
PctI GAATGC 2 cut(s) 719, 1676
PdmI GAANNNNTTC 1 cut(s) 586
PfeI GAWTC 4 cut(s) 251, 1740, 1760, 1955
PflMI CCANNNNNTGG 1 cut(s) 542
PkrI GCNGC 2 cut(s) 939, 1277
PleI GAGTC 3 cut(s) 837, 856, 1938
PpsI GAGTC 3 cut(s) 837, 856, 1938
PshBI ATTAAT 2 cut(s) 564, 927
Psp6I CCWGG 4 cut(s) 117, 463, 786, 1051
PspFI CCCAGC 2 cut(s) 145, 535
PspGI CCWGG 4 cut(s) 117, 463, 786, 1051
PspN4I GGNNCC 1 cut(s) 964
PspPI GGNCC 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 633
PsuI RGATCY 1 cut(s) 816
PvuII CAGCTG 1 cut(s) 128
RruI TCGCGA 1 cut(s) 234
RseI CAYNNNNRTG 1 cut(s) 1811
SalI GTCGAC 1 cut(s) 303
SaqAI TTAA 8 cut(s) 267, 557, 564, 927, 1368, 1424, 1445, 1754
SatI GCNGC 2 cut(s) 938, 1276
Sau3AI GATC 6 cut(s) 229, 816, 1428, 1650, 1690, 1846
Sau96I GGNCC 1 cut(s) 790
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 3 cut(s) 838, 857, 1939
ScrFI CCNGG 5 cut(s) 119, 465, 788, 1053, 1550
SduI GDGCHC 1 cut(s) 1141
SfaNI GCATC 6 cut(s) 196, 555, 657, 1262, 1793, 1939
SfcI CTRYAG 1 cut(s) 957
Sfr274I CTCGAG 1 cut(s) 1417
SinI GGWCC 1 cut(s) 790
SlaI CTCGAG 1 cut(s) 1417
SmiMI CAYNNNNRTG 1 cut(s) 1811
SmlI CTYRAG 3 cut(s) 398, 556, 1417
SmoI CTYRAG 3 cut(s) 398, 556, 1417
SphI GCATGC 1 cut(s) 265
SsiI CCGC 1 cut(s) 1023
SspI AATATT 3 cut(s) 797, 1459, 1672
StyD4I CCNGG 5 cut(s) 117, 463, 786, 1051, 1548
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 5 cut(s) 302, 506, 899, 1439, 1472
TaiI ACGT 2 cut(s) 101, 875
TaqI TCGA 4 cut(s) 304, 1190, 1418, 1849
TatI WGTACW 4 cut(s) 684, 863, 881, 1311
TfiI GAWTC 4 cut(s) 251, 1740, 1760, 1955
Tru1I TTAA 8 cut(s) 267, 557, 564, 927, 1368, 1424, 1445, 1754
Tru9I TTAA 8 cut(s) 267, 557, 564, 927, 1368, 1424, 1445, 1754
TscAI CASTG 7 cut(s) 784, 1173, 1233, 1477, 1773, 1795, 1951
TseFI GTSAC 1 cut(s) 1472
TseI GCWGC 2 cut(s) 937, 1275
Tsp45I GTSAC 1 cut(s) 1472
TspDTI ATGAA 8 cut(s) 51, 174, 243, 485, 785, 1173, 1493, 1947
TspRI CASTG 7 cut(s) 784, 1173, 1233, 1477, 1773, 1795, 1951
Van91I CCANNNNNTGG 1 cut(s) 542
Vha464I CTTAAG 1 cut(s) 556
VpaK11BI GGWCC 1 cut(s) 790
VspI ATTAAT 2 cut(s) 564, 927
XapI RAATTY 6 cut(s) 189, 283, 388, 667, 994, 1512
XceI RCATGY 2 cut(s) 265, 1318
XhoI CTCGAG 1 cut(s) 1417
XmiI GTMKAC 1 cut(s) 304
XmnI GAANNNNTTC 1 cut(s) 586
ZrmI AGTACT 1 cut(s) 865
Zsp2I ATGCAT 1 cut(s) 1808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.