RchiOBHm_Chr6g0298451

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000509
Physical Location & Seq
Reverse (-)
59881548 .. 59884770
3223 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26792

Sequence Viewer

Length: 2388 bp
ATGGCCATGTTTCTTCCTCTAATTTGCTTGATATCATCATTCTTTAGCTTTCTTTCGTTTTCATCTCGTGCAACAGATACTCTTACACCTTCAGACACGTTGAGAGATGGTCAAATCCTGGTTTCAGCTGGTGGAGTTTTTGAGCTTGGCTTCTTCGCTGAAAGCTTTTCAGGCTATCACTATCTGGGAATTTGGTTGAGAGCTGATGCAAGCAAGGTTGTCTGGGTTGATCGCGATACTCCCATATTGGATTCATCAGGCATGCTTCAAATCCTGTCAGGAAATTTGGTTCTCACAGACCGTCGACAAGTGCAATTGATAGTCAACACAGGAAGTCTTGCCACGGCCACAACTGCTAACGCAAGTGCAACACTTCTTGATACTGGAAATTTTGTCCTCAAGGAAGCAGATACAGGTAATACTATATGGCAAAGTTTTGATATACCGAGTGATACTTATCTTCCTGGGATGAAACTTGGGTTGTTTGGCCGAAACATATTTCACAGTATTGTTTCTTGGGCAAGCCCCCAAAACCCAGCTCGTGGCATCTTCACCTTAAGCATTAATAGCAACGACACCAGAAAGCTTTCAGTTCAGCGAGGAAATAATGGAGAGAAAATAGAAATCAGTTTCTGGGATGGAAATGGCATCCGGTTTATATTTGAAAATTCAACTGCCACTATGTACAATTTTAGCTTCCAGTCCAATGCAACCGAAGCATTCTACACTTTTACTACCAGTAAGAATTATGACCTTGTGTGGTTTGTGATGAATTACACTGGAAACCTGGACCAATATTTTATGGTGGATGGGAAGATCTCGTCTGTGAGTCATGCTTTGTGTGAGGCGTCAGCCGGTGGTAGTACTCGGACGTGTTCTAGTACAATACCTTCTAACTGTAAAGATGGTGCTGACTTTTCTCAGGCTAATGGTTTGCTGCCATCTACATCTAATACTACAGGTTCCCTAAATATGGTGATTACTGATTGTGAAACTTTCTGCAAGAACAATTGTTCTTGTACCGCGTTCGCTTCAGTTCAGAATGGTCAACCAGGATGTCAACTTTACTATGGGAGTAAACAGGATTTATTGAAGAACATAGAGAAGGGGGCAGGGCTTATTTACATCCGGGGTGGTGCTCCAAGTGATGCTAAGAAGTGGAAACTCAGGTTGGCTGTGGCGATTCCTGTAGCTTCTCTCTTGGTTCTTATTCCAATCTTCTTCACCTGCTACCTGTGCTGGAGAAAGCGACTGAAAGAACAAACTCAACAAGAAGCCATTTTCAATTCTGATCAAGTGAGATTGTTCCATGCGTTGCCATTTCATCACCATGAAGTTGGAGGTGTGAATAACATGGAACTAGGCATACACAAGGATAAAGAATTGCCCCTTTTTAGTTTTGCCACCATAAAGAAGGCAACAAATTACTTTGCAGATTCTAATAAACTTGGAGAAGGTGGATATGGGCCTGTCTATAAGGCAAGAATCGTTGAAGGACATGAAATTGCAGTGAAAAGGCTGTCCAAGATTTCCAGGCAAGGGTTGAGGGAGTTCAAAAACGAAGTCTCACTGATTTGTAAGCTTCAACATAGAAACTTGGTTAGGCTTCTGGGATGCTGCATTGAAGCAGAAGAAAGCATACTGATTTACGAGTACATGCCCAACAAAAGTCTAGATTTCTTCATTTTTGACTCAACCAAACAGGTACTTTTGGATTGGAGAAGGCGTGTTAACATTATTGAAGGGATTGCACAAGGTCTTCTATATCTTCACAAATACTCGAGATTAAGGATCATTCACCGTGATTTAAAGGCCAGCAATATTCTGCTGGACGGTGACATGAACCCCAAGATTTCTGATTTTGGCATGGCAAGAATTTTCAGGGACAATGACATTAGAGGAAAAACAAACCGGGTTGTTGGTACATTTGGTTATATGTCTCCTGAGTACGCAATGGGCGGTCTCTTTTCTGAAAAATCCGATGTCTTTAGCTTTGGGGTGATCTTATTAGAGATCATAAGCGGCGAGAAGAATATTGCATTCTTTGAGGCTGATCACTCTCTGAACTTACTCAGCAAAGCTTGGCATTTGTGGAAAGAAGGAAGCAGCATGGAGTTAATGGATTCAACACTGCGTGCTTCGTGTTCAAGCAGTGAAGTTATCAGATGCATACAGATGGGTCTTCTGTGCGTACAAGAAAAAGCTATTGATCGACCAACCATGTCGGATGTTGTTTCTATGCTAAGCAATCAGCCATTTGCTCTGCCTCTTCCCAAAGAACCTGCATTTTTGAGTCATTCAAGCTCCACTGATGCAGATTCATCTTCCAGTAGGCAAAGATATTACTCTAAAAATGATATGACAATTTCAGAAGAATGTGGCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

795

Amino Acids

88.52

Weight (kDa)

7.19

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 173 1e-22 D-mannose binding lectin
PAN_2 PF08276 312 - 356 7e-06 PAN-like domain
Pkinase PF00069 479 - 745 1.3e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 481 - 748 8e-47 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 754 - 790 2.1e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000509)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29542 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29544 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29560
malus_domestica MD15G1121300.v1.1
prunus_persica Prupe.1G473900_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474100_v2.0.a1 Prupe.1G474300_v2.0.a1 Prupe.1G474500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298411 RchiOBHm_Chr6g0298421 RchiOBHm_Chr6g0298431 RchiOBHm_Chr6g0298441 RchiOBHm_Chr6g0298451 RchiOBHm_Chr6g0298461 RchiOBHm_Chr6g0298471 RchiOBHm_Chr6g0298481 RchiOBHm_Chr6g0298491 RchiOBHm_Chr6g0298501 RchiOBHm_Chr6g0298521
rosa_laevigata RLG00000011506 RLG00000011508 RLG00000011509 RLG00000011511 RLG00000011513 RLG00000011514 RLG00000011515
rosa_multiflora Rmu_co8314937.1_g000001 Rmu_co8319199.1_g000001 Rmu_sc0004500.1_g000002 Rmu_sc0006543.1_g000001 Rmu_ssc0000289.1_g000039 Rmu_ssc0000289.1_g000040 Rmu_ssc0000289.1_g000047 Rmu_ssc0000289.1_g000048 Rmu_ssc0000289.1_g000049 Rmu_ssc0000289.1_g000051
rosa_roxburghii Rroxscaffold_7G00169570 Rroxscaffold_7G00169580 Rroxscaffold_7G00169600 Rroxscaffold_7G00169610 Rroxscaffold_7G00169620 Rroxscaffold_7G00169630 Rroxscaffold_7G00169650 Rroxscaffold_7G00169660 Rroxscaffold_7G00169670
rosa_rugosa Rorug06G0280700 Rorug06G0280800 Rorug06G0280900
rosa_samantha Rh6AG391700 Rh6AG391800 Rh6AG392000 Rh6AG392100 Rh6AG392200 Rh6BG399300 Rh6BG399400 Rh6BG399500 Rh6BG399700 Rh6BG400000 Rh6CG405400 Rh6CG405500 Rh6CG405600 Rh6CG405700 Rh6CG405800 Rh6DG391800 Rh6DG391900 Rh6DG392000 Rh6DG392100 Rh6DG392300
rosa_wichuraiana Rw6G034230 Rw6G034240 Rw6G034250 Rw6G034260 Rw6G034270 Rw6G034280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1235
Acc36I ACCTGC 3 cut(s) 1235, 2290, 2373
AccB7I CCANNNNNTGG 1 cut(s) 542
AccI GTMKAC 1 cut(s) 304
AccII CGCG 2 cut(s) 234, 1025
AciI CCGC 3 cut(s) 1023, 1959, 2022
AclWI GGATC 1 cut(s) 1799
AcoI YGGCCR 3 cut(s) 3, 345, 487
AcsI RAATTY 5 cut(s) 189, 283, 388, 667, 1875
AcuI CTGAAG 2 cut(s) 75, 1017
AcyI GRCGYC 1 cut(s) 848
AdeI CACNNNGTG 1 cut(s) 2135
AfaI GTAC 9 cut(s) 686, 865, 883, 1021, 1655, 1707, 1924, 1949, 2193
AfiI CCNNNNNNNGG 3 cut(s) 542, 973, 1539
AflII CTTAAG 1 cut(s) 556
AflIII ACRYGT 2 cut(s) 96, 872
AjiI CACGTC 1 cut(s) 873
AjnI CCWGG 5 cut(s) 117, 463, 786, 1051, 1532
AjuI GAANNNNNNNTTGG 2 cut(s) 1154, 1186
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
Alw21I GWGCWC 1 cut(s) 1141
Alw26I GTCTC 3 cut(s) 1570, 1944, 1967
AlwI GGATC 1 cut(s) 1799
AlwNI CAGNNNCTG 1 cut(s) 633
Ama87I CYCGRG 1 cut(s) 1780
AoxI GGCC 5 cut(s) 3, 345, 487, 1466, 1812
ApeKI GCWGC 3 cut(s) 937, 1617, 2106
ApoI RAATTY 5 cut(s) 189, 283, 388, 667, 1875
ArsI GACNNNNNNTTYG 2 cut(s) 1548, 1580
AseI ATTAAT 1 cut(s) 564
Asp700I GAANNNNTTC 1 cut(s) 586
AspS9I GGNCC 2 cut(s) 790, 1466
AsuC2I CCSGG 2 cut(s) 1130, 1913
AsuHPI GGTGA 7 cut(s) 544, 988, 1216, 1319, 1790, 1847, 2011
AvaI CYCGRG 1 cut(s) 1780
AvaII GGWCC 1 cut(s) 790
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 4 cut(s) 874, 906, 1623, 1655
BauI CACGAG 2 cut(s) 66, 540
BbsI GAAGAC 2 cut(s) 1751, 2174
Bbv12I GWGCWC 1 cut(s) 1141
BbvI GCAGC 3 cut(s) 924, 1604, 2118
BccI CCATC 6 cut(s) 101, 632, 803, 899, 949, 2170
BceAI ACGGC 1 cut(s) 360
BciT130I CCWGG 5 cut(s) 119, 465, 788, 1053, 1534
BclI TGATCA 2 cut(s) 1291, 2053
BcnI CCSGG 2 cut(s) 1130, 1913
BcoDI GTCTC 3 cut(s) 1570, 1944, 1967
BfaI CTAG 3 cut(s) 879, 1361, 1673
BfmI CTRYAG 2 cut(s) 957, 1188
BfrI CTTAAG 1 cut(s) 556
BfuAI ACCTGC 3 cut(s) 1235, 2290, 2373
BglII AGATCT 1 cut(s) 816
BisI GCNGC 4 cut(s) 938, 1618, 2023, 2107
BlpI GCTNAGC 1 cut(s) 2243
BlsI GCNGC 4 cut(s) 939, 1619, 2024, 2108
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 7 cut(s) 119, 465, 788, 1053, 1130, 1534, 1913
Bme18I GGWCC 1 cut(s) 790
BmeT110I CYCGRG 1 cut(s) 1780
BmgBI CACGTC 1 cut(s) 873
BmgT120I GGNCC 2 cut(s) 790, 1466
BmiI GGNNCC 1 cut(s) 964
BmrFI CCNGG 7 cut(s) 119, 465, 788, 1053, 1130, 1534, 1913
BmsI GCATC 7 cut(s) 196, 555, 657, 1138, 1604, 2156, 2302
BpiI GAAGAC 2 cut(s) 1751, 2174
BpmI CTGGAG 1 cut(s) 1261
Bpu1102I GCTNAGC 1 cut(s) 2243
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 2 cut(s) 1130, 1913
BsaBI GATNNNNATC 1 cut(s) 456
BsaHI GRCGYC 1 cut(s) 848
BsaI GGTCTC 1 cut(s) 1967
BsaJI CCNNGG 3 cut(s) 342, 464, 1129
BsaWI WCCGGW 1 cut(s) 651
BsaXI ACNNNNNCTCC 2 cut(s) 1711, 1741
Bsc4I CCNNNNNNNGG 3 cut(s) 542, 973, 1539
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 5 cut(s) 388, 700, 738, 784, 2328
Bse3DI GCAATG 1 cut(s) 1959
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 5 cut(s) 119, 465, 788, 1053, 1534
BseDI CCNNGG 3 cut(s) 342, 464, 1129
BseGI GGATG 8 cut(s) 474, 643, 648, 814, 1061, 1125, 1619, 2233
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 3 cut(s) 542, 973, 1539
BseMI GCAATG 1 cut(s) 1959
BseMII CTCAG 4 cut(s) 935, 1180, 1935, 2086
BseNI ACTGG 5 cut(s) 388, 700, 738, 784, 2328
BseXI GCAGC 3 cut(s) 924, 1604, 2118
BseYI CCCAGC 1 cut(s) 535
Bsh1236I CGCG 2 cut(s) 234, 1025
BshFI GGCC 5 cut(s) 5, 347, 489, 1468, 1814
BsiHKAI GWGCWC 1 cut(s) 1141
BsiHKCI CYCGRG 1 cut(s) 1780
BsiSI CCGG 4 cut(s) 652, 855, 1129, 1912
BslFI GGGAC 1 cut(s) 1898
BslI CCNNNNNNNGG 3 cut(s) 542, 973, 1539
BsmAI GTCTC 3 cut(s) 1570, 1944, 1967
BsmFI GGGAC 1 cut(s) 1898
BsmI GAATGC 2 cut(s) 719, 2039
BsnI GGCC 5 cut(s) 5, 347, 489, 1468, 1814
Bso31I GGTCTC 1 cut(s) 1967
BsoBI CYCGRG 1 cut(s) 1780
Bsp1286I GDGCHC 1 cut(s) 1141
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 8 cut(s) 229, 816, 1291, 1791, 2001, 2013, 2053, 2209
Bsp1720I GCTNAGC 1 cut(s) 2243
Bsp68I TCGCGA 1 cut(s) 234
BspACI CCGC 3 cut(s) 1023, 1959, 2022
BspANI GGCC 5 cut(s) 5, 347, 489, 1468, 1814
BspCNI CTCAG 4 cut(s) 934, 1179, 1936, 2085
BspFNI CGCG 2 cut(s) 234, 1025
BspLI GGNNCC 1 cut(s) 964
BspMI ACCTGC 3 cut(s) 1235, 2290, 2373
BspPI GGATC 1 cut(s) 1799
BspTI CTTAAG 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 1967
BsrDI GCAATG 1 cut(s) 1959
BsrFI RCCGGY 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 5 cut(s) 388, 700, 738, 784, 2328
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 3 cut(s) 342, 464, 1129
BssMI GATC 8 cut(s) 229, 816, 1291, 1791, 2001, 2013, 2053, 2209
BssNI GRCGYC 1 cut(s) 848
BssSI CACGAG 2 cut(s) 66, 540
Bst2BI CACGAG 2 cut(s) 66, 540
Bst2UI CCWGG 5 cut(s) 119, 465, 788, 1053, 1534
Bst4CI ACNGT 5 cut(s) 302, 506, 899, 1802, 1835
Bst6I CTCTTC 1 cut(s) 2274
BstACI GRCGYC 1 cut(s) 848
BstAFI CTTAAG 1 cut(s) 556
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 5 cut(s) 211, 263, 523, 1816, 2136
BstDEI CTNAG 6 cut(s) 921, 1152, 1166, 1944, 2072, 2243
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 8 cut(s) 474, 643, 648, 814, 1061, 1125, 1619, 2233
BstFNI CGCG 2 cut(s) 234, 1025
BstKTI GATC 8 cut(s) 232, 819, 1294, 1794, 2004, 2016, 2056, 2212
BstMAI GTCTC 3 cut(s) 1570, 1944, 1967
BstMBI GATC 8 cut(s) 229, 816, 1291, 1791, 2001, 2013, 2053, 2209
BstMWI GCNNNNNNNGC 5 cut(s) 171, 353, 567, 716, 1236
BstNI CCWGG 5 cut(s) 119, 465, 788, 1053, 1534
BstNSI RCATGY 2 cut(s) 265, 1660
BstSCI CCNGG 7 cut(s) 117, 463, 786, 1051, 1128, 1532, 1911
BstSFI CTRYAG 2 cut(s) 957, 1188
BstUI CGCG 2 cut(s) 234, 1025
BstV1I GCAGC 3 cut(s) 924, 1604, 2118
BstV2I GAAGAC 2 cut(s) 1751, 2174
BstX2I RGATCY 1 cut(s) 816
BstXI CCANNNNNNTGG 1 cut(s) 1337
BstYI RGATCY 1 cut(s) 816
BsuRI GGCC 5 cut(s) 5, 347, 489, 1468, 1814
BtgI CCRYGG 1 cut(s) 342
BtrI CACGTC 1 cut(s) 873
BtsCI GGATG 8 cut(s) 474, 643, 648, 814, 1061, 1125, 1619, 2233
BtsI GCAGTG 3 cut(s) 1515, 2129, 2158
BtsIMutI CAGTG 6 cut(s) 777, 1515, 1568, 2129, 2158, 2307
BtuMI TCGCGA 1 cut(s) 234
BveI ACCTGC 3 cut(s) 1235, 2290, 2373
Cac8I GCNNGC 5 cut(s) 211, 263, 523, 1816, 2136
CaiI CAGNNNCTG 1 cut(s) 633
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 2 cut(s) 790, 1466
CseI GACGC 1 cut(s) 837
Csp6I GTAC 9 cut(s) 685, 864, 882, 1020, 1654, 1706, 1923, 1948, 2192
CviQI GTAC 9 cut(s) 685, 864, 882, 1020, 1654, 1706, 1923, 1948, 2192
DdeI CTNAG 6 cut(s) 921, 1152, 1166, 1944, 2072, 2243
DpnI GATC 8 cut(s) 231, 818, 1293, 1793, 2003, 2015, 2055, 2211
DpnII GATC 8 cut(s) 229, 816, 1291, 1791, 2001, 2013, 2053, 2209
DraI TTTAAA 1 cut(s) 1809
DraIII CACNNNGTG 1 cut(s) 2135
EaeI YGGCCR 3 cut(s) 3, 345, 487
Eam1104I CTCTTC 1 cut(s) 2274
EarI CTCTTC 1 cut(s) 2274
Eco31I GGTCTC 1 cut(s) 1967
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 790
Eco57I CTGAAG 2 cut(s) 75, 1017
Eco88I CYCGRG 1 cut(s) 1780
EcoRII CCWGG 5 cut(s) 117, 463, 786, 1051, 1532
EcoRV GATATC 1 cut(s) 33
EcoT22I ATGCAT 1 cut(s) 2171
FaqI GGGAC 1 cut(s) 1898
FbaI TGATCA 2 cut(s) 1291, 2053
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 938, 1618, 2023, 2107
FokI GGATG 8 cut(s) 481, 635, 650, 821, 1068, 1112, 1626, 2240
Fsp4HI GCNGC 4 cut(s) 938, 1618, 2023, 2107
FspBI CTAG 3 cut(s) 879, 1361, 1673
GluI GCNGC 4 cut(s) 938, 1618, 2023, 2107
GsaI CCCAGC 1 cut(s) 539
GsuI CTGGAG 1 cut(s) 1261
HaeIII GGCC 5 cut(s) 5, 347, 489, 1468, 1814
HapII CCGG 4 cut(s) 652, 855, 1129, 1912
HgaI GACGC 1 cut(s) 837
Hin1I GRCGYC 1 cut(s) 848
HincII GTYRAC 5 cut(s) 305, 325, 1049, 1061, 1732
HindII GTYRAC 5 cut(s) 305, 325, 1049, 1061, 1732
HindIII AAGCTT 4 cut(s) 163, 584, 1580, 2079
HinfI GANTC 9 cut(s) 251, 829, 1183, 1436, 1485, 1691, 2123, 2293, 2318
HpaI GTTAAC 1 cut(s) 1732
HpaII CCGG 4 cut(s) 652, 855, 1129, 1912
HphI GGTGA 7 cut(s) 544, 988, 1216, 1319, 1790, 1847, 2011
Hpy166II GTNNAC 6 cut(s) 305, 325, 1049, 1061, 1079, 1732
Hpy188III TCNNGA 6 cut(s) 233, 279, 377, 1673, 1782, 1943
Hpy8I GTNNAC 6 cut(s) 305, 325, 1049, 1061, 1079, 1732
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 9 cut(s) 99, 900, 1099, 1408, 1448, 1487, 1716, 1736, 2093
HpyCH4III ACNGT 5 cut(s) 302, 506, 899, 1802, 1835
HpyCH4IV ACGT 2 cut(s) 98, 872
HpyF10VI GCNNNNNNNGC 5 cut(s) 171, 353, 567, 716, 1236
HpyF3I CTNAG 6 cut(s) 921, 1152, 1166, 1944, 2072, 2243
HpySE526I ACGT 2 cut(s) 98, 872
Hsp92I GRCGYC 1 cut(s) 848
Ksp22I TGATCA 2 cut(s) 1291, 2053
KspAI GTTAAC 1 cut(s) 1732
Kzo9I GATC 8 cut(s) 229, 816, 1291, 1791, 2001, 2013, 2053, 2209
LmnI GCTCC 2 cut(s) 1144, 2309
Lsp1109I GCAGC 3 cut(s) 924, 1604, 2118
LweI GCATC 7 cut(s) 196, 555, 657, 1138, 1604, 2156, 2302
MaeI CTAG 3 cut(s) 879, 1361, 1673
MaeII ACGT 2 cut(s) 98, 872
MaeIII GTNAC 1 cut(s) 1835
MalI GATC 8 cut(s) 231, 818, 1293, 1793, 2003, 2015, 2055, 2211
MboI GATC 8 cut(s) 229, 816, 1291, 1791, 2001, 2013, 2053, 2209
MfeI CAATTG 2 cut(s) 314, 1009
MflI RGATCY 1 cut(s) 816
MhlI GDGCHC 1 cut(s) 1141
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 838, 1685, 2302
MmeI TCCRAC 2 cut(s) 1318, 2205
MnlI CCTC 9 cut(s) 27, 407, 593, 838, 1334, 1539, 1892, 2041, 2277
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 2171
MroXI GAANNNNTTC 1 cut(s) 586
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 557, 564, 1731, 1787, 1808, 2117
MslI CAYNNNNRTG 2 cut(s) 1329, 2174
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 128
MspCI CTTAAG 1 cut(s) 556
MspI CCGG 4 cut(s) 652, 855, 1129, 1912
MspR9I CCNGG 7 cut(s) 119, 465, 788, 1053, 1130, 1534, 1913
MunI CAATTG 2 cut(s) 314, 1009
Mva1269I GAATGC 2 cut(s) 719, 2039
MvaI CCWGG 5 cut(s) 119, 465, 788, 1053, 1534
MvnI CGCG 2 cut(s) 234, 1025
MwoI GCNNNNNNNGC 5 cut(s) 171, 353, 567, 716, 1236
NciI CCSGG 2 cut(s) 1130, 1913
NdeII GATC 8 cut(s) 229, 816, 1291, 1791, 2001, 2013, 2053, 2209
NlaIV GGNNCC 1 cut(s) 964
NmuCI GTSAC 1 cut(s) 1835
NruI TCGCGA 1 cut(s) 234
NsiI ATGCAT 1 cut(s) 2171
NspI RCATGY 2 cut(s) 265, 1660
PaeI GCATGC 1 cut(s) 265
PaeR7I CTCGAG 1 cut(s) 1780
PaqCI CACCTGC 1 cut(s) 1235
PctI GAATGC 2 cut(s) 719, 2039
PdmI GAANNNNTTC 1 cut(s) 586
PfeI GAWTC 6 cut(s) 251, 1183, 1436, 1485, 2123, 2318
PflMI CCANNNNNTGG 1 cut(s) 542
PkrI GCNGC 4 cut(s) 939, 1619, 2024, 2108
PleI GAGTC 3 cut(s) 837, 1685, 2301
PpsI GAGTC 3 cut(s) 837, 1685, 2301
PshBI ATTAAT 1 cut(s) 564
Psp6I CCWGG 5 cut(s) 117, 463, 786, 1051, 1532
PspFI CCCAGC 1 cut(s) 535
PspGI CCWGG 5 cut(s) 117, 463, 786, 1051, 1532
PspN4I GGNNCC 1 cut(s) 964
PspPI GGNCC 2 cut(s) 790, 1466
PsrI GAACNNNNNNTAC 2 cut(s) 1350, 1382
PstNI CAGNNNCTG 1 cut(s) 633
PsuI RGATCY 1 cut(s) 816
PvuII CAGCTG 1 cut(s) 128
RruI TCGCGA 1 cut(s) 234
RsaI GTAC 9 cut(s) 686, 865, 883, 1021, 1655, 1707, 1924, 1949, 2193
RsaNI GTAC 9 cut(s) 685, 864, 882, 1020, 1654, 1706, 1923, 1948, 2192
RseI CAYNNNNRTG 2 cut(s) 1329, 2174
SalI GTCGAC 1 cut(s) 303
SaqAI TTAA 6 cut(s) 557, 564, 1731, 1787, 1808, 2117
SatI GCNGC 4 cut(s) 938, 1618, 2023, 2107
Sau3AI GATC 8 cut(s) 229, 816, 1291, 1791, 2001, 2013, 2053, 2209
Sau96I GGNCC 2 cut(s) 790, 1466
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 3 cut(s) 838, 1685, 2302
ScrFI CCNGG 7 cut(s) 119, 465, 788, 1053, 1130, 1534, 1913
SduI GDGCHC 1 cut(s) 1141
SfaNI GCATC 7 cut(s) 196, 555, 657, 1138, 1604, 2156, 2302
SfcI CTRYAG 2 cut(s) 957, 1188
Sfr274I CTCGAG 1 cut(s) 1780
SinI GGWCC 1 cut(s) 790
SlaI CTCGAG 1 cut(s) 1780
SmiMI CAYNNNNRTG 2 cut(s) 1329, 2174
SmlI CTYRAG 3 cut(s) 398, 556, 1780
SmoI CTYRAG 3 cut(s) 398, 556, 1780
SphI GCATGC 1 cut(s) 265
SsiI CCGC 3 cut(s) 1023, 1959, 2022
SspI AATATT 3 cut(s) 797, 1822, 2035
SspMI CTAG 3 cut(s) 879, 1361, 1673
StyD4I CCNGG 7 cut(s) 117, 463, 786, 1051, 1128, 1532, 1911
TaaI ACNGT 5 cut(s) 302, 506, 899, 1802, 1835
TaiI ACGT 2 cut(s) 101, 875
TaqI TCGA 3 cut(s) 304, 1781, 2212
TatI WGTACW 4 cut(s) 684, 863, 881, 1653
TauI GCSGC 1 cut(s) 2025
TfiI GAWTC 6 cut(s) 251, 1183, 1436, 1485, 2123, 2318
Tru1I TTAA 6 cut(s) 557, 564, 1731, 1787, 1808, 2117
Tru9I TTAA 6 cut(s) 557, 564, 1731, 1787, 1808, 2117
TscAI CASTG 6 cut(s) 784, 1515, 1575, 2136, 2158, 2314
TseFI GTSAC 1 cut(s) 1835
TseI GCWGC 3 cut(s) 937, 1617, 2106
Tsp45I GTSAC 1 cut(s) 1835
TspRI CASTG 6 cut(s) 784, 1515, 1575, 2136, 2158, 2314
Van91I CCANNNNNTGG 1 cut(s) 542
Vha464I CTTAAG 1 cut(s) 556
VpaK11BI GGWCC 1 cut(s) 790
VspI ATTAAT 1 cut(s) 564
XapI RAATTY 5 cut(s) 189, 283, 388, 667, 1875
XbaI TCTAGA 1 cut(s) 1672
XceI RCATGY 2 cut(s) 265, 1660
XhoI CTCGAG 1 cut(s) 1780
XmiI GTMKAC 1 cut(s) 304
XmnI GAANNNNTTC 1 cut(s) 586
XspI CTAG 3 cut(s) 879, 1361, 1673
ZrmI AGTACT 1 cut(s) 865
Zsp2I ATGCAT 1 cut(s) 2171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.