Rh6BG400000

Domain of unknown function (DUF3403)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000509
Physical Location & Seq
Reverse (-)
62752104 .. 62755275
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG400000.1

Sequence Viewer

Length: 1278 bp
ATGGCAATGGCTCTTCCTCTTATTATTTGCTTGATATTATTCTCAAGTTTGTTTCCATTTCCATGCCATGCAATCACAGACACGCTCACAGCTTCAGACTCGTTGAGCGACAACCAAACGCTGGTTTCTGCAGGCGGAGTCTTCGTCCTTGGCTTCTTCATCGATAGCTCTTCAGGTAATCACTATCTAGGTATATGGCTGAGAGCTGATGCAAAGAGAGTTGTTTGGGTTGCCTACCGTGAAATACCTATATTGGACTCTTCCGGCGTGCTTCAAATCCGGTCAGGGAATCTGGTTGTCACCGACCGACGACAACTGCAGCTCATAGTCAACTCGGGAAACGTTGCCGCCACCACCAACACGAGTGCAACACTTCTTGATACGGGAAATCTTGTGCTTAAGGATGAAAATACAGGTACCACTGTATGGCAGAGCTTCGATGTACCAACTGATACATATCTTCCGGGGATGAAAGTTGGGTTGTTTGGCCTAAACACAAGCCAACAAAGCTTTCGGATTCTCACTTCCTGGGAAAGCCCTCAAAACCCTAGTCGTGGCCTCTTCACCTTCTCGCTGGATAGAATAGACTTCACTAAAATTGATGTTTGGCGAGGAGATGGACTCCAAATGCATATCGCTTTCTGGGACGGACATGATCTCCGGTTCATTTTCGAGAACACAACCGCAAACAATGACTACAACTTTAGCTACACTTCCATTAATGGAAGTGAAGCCTACTACACTTTTAGCCACAACAAGAAGTATGACCTCATGTGGTTTGTAATGGCTTCAACAGGAAACCTAGACCAGTACTTCATGGTGAATGGGAAGATTACTTCTACGAGTCATGCTTTGTGTGAAGATTCAAGTGGTGGTAATAGTGGAAAGTGCTTGACTTCTTTACCACCTATGTGTGGTAATACTTTTAACCAGACGAATGGTTCGTTGCCGTCGACGGTTAAGGTTGACTCAATTAATATAGGGGCTAATGATTGTGAGACTTTGTGCAAGAGCAATTGTTCTTGTACTGCATATGTTTCAGTTCAGAATGATCAGTCGGTTTGTCAACTTTATTATGGGAGTAAACAGGAGTTGATGAAGATCATGGAGATAGGGTCAGGGACTATCAACGTTCGTGGTTCTGCTAATACAAGTGATGGTAAGAAGTGGAAAAGGTGGTTGACTATCGTGCTCCCGGTGGTTTTCCTATCGGCTCTTATTTCAGTCTTCTTATTCTGTTATCTAGGTTGGAGAAGAGGACCTCAAGGTTTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

425

Amino Acids

46.84

Weight (kDa)

5.87

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 177 6.9e-27 D-mannose binding lectin
PAN_2 PF08276 315 - 357 6.3e-06 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000509)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29542 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29544 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29560
malus_domestica MD15G1121300.v1.1
prunus_persica Prupe.1G473900_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474100_v2.0.a1 Prupe.1G474300_v2.0.a1 Prupe.1G474500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298411 RchiOBHm_Chr6g0298421 RchiOBHm_Chr6g0298431 RchiOBHm_Chr6g0298441 RchiOBHm_Chr6g0298451 RchiOBHm_Chr6g0298461 RchiOBHm_Chr6g0298471 RchiOBHm_Chr6g0298481 RchiOBHm_Chr6g0298491 RchiOBHm_Chr6g0298501 RchiOBHm_Chr6g0298521
rosa_laevigata RLG00000011506 RLG00000011508 RLG00000011509 RLG00000011511 RLG00000011513 RLG00000011514 RLG00000011515
rosa_multiflora Rmu_co8314937.1_g000001 Rmu_co8319199.1_g000001 Rmu_sc0004500.1_g000002 Rmu_sc0006543.1_g000001 Rmu_ssc0000289.1_g000039 Rmu_ssc0000289.1_g000040 Rmu_ssc0000289.1_g000047 Rmu_ssc0000289.1_g000048 Rmu_ssc0000289.1_g000049 Rmu_ssc0000289.1_g000051
rosa_roxburghii Rroxscaffold_7G00169570 Rroxscaffold_7G00169580 Rroxscaffold_7G00169600 Rroxscaffold_7G00169610 Rroxscaffold_7G00169620 Rroxscaffold_7G00169630 Rroxscaffold_7G00169650 Rroxscaffold_7G00169660 Rroxscaffold_7G00169670
rosa_rugosa Rorug06G0280700 Rorug06G0280800 Rorug06G0280900
rosa_samantha Rh6AG391700 Rh6AG391800 Rh6AG392000 Rh6AG392100 Rh6AG392200 Rh6BG399300 Rh6BG399400 Rh6BG399500 Rh6BG399700 Rh6BG400000 Rh6CG405400 Rh6CG405500 Rh6CG405600 Rh6CG405700 Rh6CG405800 Rh6DG391800 Rh6DG391900 Rh6DG392000 Rh6DG392100 Rh6DG392300
rosa_wichuraiana Rw6G034230 Rw6G034240 Rw6G034250 Rw6G034260 Rw6G034270 Rw6G034280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 416
AccB7I CCANNNNNTGG 2 cut(s) 121, 426
AccI GTMKAC 1 cut(s) 953
AciI CCGC 3 cut(s) 135, 348, 684
AclI AACGTT 2 cut(s) 342, 1131
AcuI CTGAAG 2 cut(s) 78, 156
AfaI GTAC 4 cut(s) 418, 444, 812, 1027
AfiI CCNNNNNNNGG 4 cut(s) 121, 426, 554, 914
AflII CTTAAG 1 cut(s) 398
AgsI TTSAA 3 cut(s) 275, 792, 867
AjnI CCWGG 1 cut(s) 527
AjuI GAANNNNNNNTTGG 2 cut(s) 495, 527
AleI CACNNNNGTG 1 cut(s) 910
AluBI AGCT 7 cut(s) 92, 168, 206, 322, 435, 510, 708
AluI AGCT 7 cut(s) 92, 168, 206, 322, 435, 510, 708
Alw21I GWGCWC 1 cut(s) 1194
Alw26I GTCTC 1 cut(s) 992
Ama87I CYCGRG 1 cut(s) 334
AoxI GGCC 2 cut(s) 487, 556
ApeKI GCWGC 1 cut(s) 319
ArsI GACNNNNNNTTYG 4 cut(s) 535, 567, 925, 957
AseI ATTAAT 2 cut(s) 720, 975
Asp718I GGTACC 1 cut(s) 416
AspS9I GGNCC 1 cut(s) 1259
AsuC2I CCSGG 2 cut(s) 465, 1196
AsuHPI GGTGA 3 cut(s) 292, 556, 832
AvaI CYCGRG 1 cut(s) 334
AvaII GGWCC 1 cut(s) 1259
BaeI ACNNNNGTAYC 2 cut(s) 408, 441
BanI GGYRCC 1 cut(s) 416
BauI CACGAG 1 cut(s) 361
BbsI GAAGAC 2 cut(s) 133, 1219
Bbv12I GWGCWC 1 cut(s) 1194
BbvI GCAGC 1 cut(s) 331
BccI CCATC 2 cut(s) 611, 1151
BceAI ACGGC 1 cut(s) 934
BciT130I CCWGG 1 cut(s) 529
BclI TGATCA 1 cut(s) 1051
BcnI CCSGG 2 cut(s) 465, 1196
BcoDI GTCTC 1 cut(s) 992
BfaI CTAG 4 cut(s) 188, 549, 803, 1244
BfmI CTRYAG 2 cut(s) 129, 317
BfrI CTTAAG 1 cut(s) 398
BisI GCNGC 2 cut(s) 320, 348
BlsI GCNGC 2 cut(s) 321, 349
BmcAI AGTACT 1 cut(s) 812
Bme1390I CCNGG 3 cut(s) 465, 529, 1196
Bme18I GGWCC 1 cut(s) 1259
BmeT110I CYCGRG 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 1259
BmiI GGNNCC 1 cut(s) 418
BmrFI CCNGG 3 cut(s) 465, 529, 1196
BmsI GCATC 1 cut(s) 199
BpiI GAAGAC 2 cut(s) 133, 1219
BplI GAGNNNNNCTC 2 cut(s) 606, 638
BpuEI CTTGAG 2 cut(s) 28, 1248
BpuMI CCSGG 2 cut(s) 465, 1196
Bsa29I ATCGAT 1 cut(s) 162
BsaBI GATNNNNATC 2 cut(s) 456, 1100
BsaJI CCNNGG 3 cut(s) 148, 464, 528
BsaWI WCCGGW 2 cut(s) 279, 660
BsaXI ACNNNNNCTCC 6 cut(s) 129, 159, 642, 672, 1100, 1130
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 426, 554, 914
Bse1I ACTGG 1 cut(s) 808
Bse3DI GCAATG 1 cut(s) 12
Bse8I GATNNNNATC 2 cut(s) 456, 1100
BseBI CCWGG 1 cut(s) 529
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 3 cut(s) 148, 464, 528
BseGI GGATG 2 cut(s) 409, 474
BseJI GATNNNNATC 2 cut(s) 456, 1100
BseLI CCNNNNNNNGG 4 cut(s) 121, 426, 554, 914
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 1 cut(s) 808
BseRI GAGGAG 1 cut(s) 627
BseXI GCAGC 1 cut(s) 331
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 2 cut(s) 489, 558
BshNI GGYRCC 1 cut(s) 416
BshVI ATCGAT 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 1194
BsiHKCI CYCGRG 1 cut(s) 334
BsiSI CCGG 5 cut(s) 264, 280, 464, 661, 1196
BslFI GGGAC 2 cut(s) 659, 1135
BslI CCNNNNNNNGG 4 cut(s) 121, 426, 554, 914
BsmAI GTCTC 1 cut(s) 992
BsmFI GGGAC 2 cut(s) 659, 1135
BsnI GGCC 2 cut(s) 489, 558
BsoBI CYCGRG 1 cut(s) 334
Bsp1286I GDGCHC 1 cut(s) 1194
Bsp143I GATC 3 cut(s) 655, 1051, 1101
BspACI CCGC 3 cut(s) 135, 348, 684
BspANI GGCC 2 cut(s) 489, 558
BspCNI CTCAG 1 cut(s) 192
BspDI ATCGAT 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 418
BspMAI CTGCAG 2 cut(s) 133, 321
BspQI GCTCTTC 2 cut(s) 18, 175
BspT107I GGYRCC 1 cut(s) 416
BspTI CTTAAG 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 1 cut(s) 808
BssECI CCNNGG 3 cut(s) 148, 464, 528
BssMI GATC 3 cut(s) 655, 1051, 1101
BssSI CACGAG 1 cut(s) 361
BssT1I CCWWGG 1 cut(s) 148
Bst2BI CACGAG 1 cut(s) 361
Bst2UI CCWGG 1 cut(s) 529
Bst4CI ACNGT 3 cut(s) 239, 424, 958
Bst6I CTCTTC 5 cut(s) 18, 175, 265, 566, 1249
BstAFI CTTAAG 1 cut(s) 398
BstC8I GCNNGC 2 cut(s) 133, 269
BstDEI CTNAG 1 cut(s) 200
BstF5I GGATG 2 cut(s) 409, 474
BstKTI GATC 3 cut(s) 658, 1054, 1104
BstMAI GTCTC 1 cut(s) 992
BstMBI GATC 3 cut(s) 655, 1051, 1101
BstMCI CGRYCG 1 cut(s) 307
BstMWI GCNNNNNNNGC 1 cut(s) 507
BstNI CCWGG 1 cut(s) 529
BstSCI CCNGG 3 cut(s) 463, 527, 1194
BstSFI CTRYAG 2 cut(s) 129, 317
BstV1I GCAGC 1 cut(s) 331
BstV2I GAAGAC 2 cut(s) 133, 1219
BstXI CCANNNNNNTGG 1 cut(s) 938
Bsu15I ATCGAT 1 cut(s) 162
BsuRI GGCC 2 cut(s) 489, 558
BsuTUI ATCGAT 1 cut(s) 162
BtsCI GGATG 2 cut(s) 409, 474
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 2 cut(s) 133, 269
Cfr13I GGNCC 1 cut(s) 1259
ClaI ATCGAT 1 cut(s) 162
Csp6I GTAC 4 cut(s) 417, 443, 811, 1026
CspCI CAANNNNNGTGG 2 cut(s) 1117, 1152
CviAII CATG 7 cut(s) 63, 68, 653, 772, 817, 848, 1105
CviQI GTAC 4 cut(s) 417, 443, 811, 1026
DdeI CTNAG 1 cut(s) 200
DpnI GATC 3 cut(s) 657, 1053, 1103
DpnII GATC 3 cut(s) 655, 1051, 1101
Eam1104I CTCTTC 5 cut(s) 18, 175, 265, 566, 1249
EarI CTCTTC 5 cut(s) 18, 175, 265, 566, 1249
EciI GGCGGA 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 1259
Eco57I CTGAAG 2 cut(s) 78, 156
Eco88I CYCGRG 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 1259
EcoRII CCWGG 1 cut(s) 527
EcoT14I CCWWGG 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 633
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 7 cut(s) 66, 71, 656, 775, 820, 851, 1108
FaqI GGGAC 2 cut(s) 659, 1135
FatI CATG 7 cut(s) 62, 67, 652, 771, 816, 847, 1104
FauNDI CATATG 1 cut(s) 1033
FbaI TGATCA 1 cut(s) 1051
FblI GTMKAC 1 cut(s) 953
Fnu4HI GCNGC 2 cut(s) 320, 348
FokI GGATG 2 cut(s) 416, 481
Fsp4HI GCNGC 2 cut(s) 320, 348
FspBI CTAG 4 cut(s) 188, 549, 803, 1244
GluI GCNGC 2 cut(s) 320, 348
HaeIII GGCC 2 cut(s) 489, 558
HapII CCGG 5 cut(s) 264, 280, 464, 661, 1196
Hin1II CATG 7 cut(s) 66, 71, 656, 775, 820, 851, 1108
HincII GTYRAC 5 cut(s) 331, 954, 967, 1067, 1182
HindII GTYRAC 5 cut(s) 331, 954, 967, 1067, 1182
HindIII AAGCTT 1 cut(s) 508
HinfI GANTC 9 cut(s) 98, 138, 257, 289, 517, 621, 844, 863, 968
HpaII CCGG 5 cut(s) 264, 280, 464, 661, 1196
HphI GGTGA 3 cut(s) 292, 556, 832
Hpy166II GTNNAC 6 cut(s) 331, 954, 967, 1067, 1085, 1182
Hpy188I TCNGA 3 cut(s) 97, 516, 1047
Hpy188III TCNNGA 3 cut(s) 336, 377, 673
Hpy8I GTNNAC 6 cut(s) 331, 954, 967, 1067, 1085, 1182
Hpy99I CGWCG 3 cut(s) 312, 955, 958
HpyAV CCTTC 1 cut(s) 577
HpyCH4III ACNGT 3 cut(s) 239, 424, 958
HpyCH4IV ACGT 2 cut(s) 342, 1131
HpyCH4V TGCA 8 cut(s) 71, 131, 212, 319, 368, 631, 1008, 1031
HpyF10VI GCNNNNNNNGC 1 cut(s) 507
HpyF3I CTNAG 1 cut(s) 200
HpySE526I ACGT 2 cut(s) 342, 1131
Hsp92II CATG 7 cut(s) 66, 71, 656, 775, 820, 851, 1108
KpnI GGTACC 1 cut(s) 420
Ksp22I TGATCA 1 cut(s) 1051
Kzo9I GATC 3 cut(s) 655, 1051, 1101
LguI GCTCTTC 2 cut(s) 18, 175
LmnI GCTCC 1 cut(s) 1197
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 1 cut(s) 199
MaeI CTAG 4 cut(s) 188, 549, 803, 1244
MaeII ACGT 2 cut(s) 342, 1131
MaeIII GTNAC 1 cut(s) 298
MalI GATC 3 cut(s) 657, 1053, 1103
MboI GATC 3 cut(s) 655, 1051, 1101
MfeI CAATTG 1 cut(s) 1015
MhlI GDGCHC 1 cut(s) 1194
MluCI AATT 3 cut(s) 597, 972, 1015
MlyI GAGTC 6 cut(s) 92, 147, 251, 615, 853, 962
MmeI TCCRAC 1 cut(s) 1229
MnlI CCTC 7 cut(s) 27, 549, 569, 605, 779, 1250, 1272
Mph1103I ATGCAT 1 cut(s) 633
MseI TTAA 6 cut(s) 399, 720, 927, 960, 975, 1276
MslI CAYNNNNRTG 2 cut(s) 61, 910
MspCI CTTAAG 1 cut(s) 398
MspI CCGG 5 cut(s) 264, 280, 464, 661, 1196
MspR9I CCNGG 3 cut(s) 465, 529, 1196
MunI CAATTG 1 cut(s) 1015
MvaI CCWGG 1 cut(s) 529
MwoI GCNNNNNNNGC 1 cut(s) 507
NciI CCSGG 2 cut(s) 465, 1196
NdeI CATATG 1 cut(s) 1033
NdeII GATC 3 cut(s) 655, 1051, 1101
NlaIII CATG 7 cut(s) 66, 71, 656, 775, 820, 851, 1108
NlaIV GGNNCC 1 cut(s) 418
NmuCI GTSAC 1 cut(s) 298
NsiI ATGCAT 1 cut(s) 633
OliI CACNNNNGTG 1 cut(s) 910
PciSI GCTCTTC 2 cut(s) 18, 175
PcsI WCGNNNNNNNCGW 2 cut(s) 941, 950
PfeI GAWTC 3 cut(s) 289, 517, 863
PflMI CCANNNNNTGG 2 cut(s) 121, 426
PkrI GCNGC 2 cut(s) 321, 349
PleI GAGTC 6 cut(s) 92, 146, 251, 615, 852, 962
PpsI GAGTC 6 cut(s) 92, 146, 251, 615, 852, 962
PpuMI RGGWCCY 1 cut(s) 1259
PshBI ATTAAT 2 cut(s) 720, 975
Psp1406I AACGTT 2 cut(s) 342, 1131
Psp5II RGGWCCY 1 cut(s) 1259
Psp6I CCWGG 1 cut(s) 527
PspGI CCWGG 1 cut(s) 527
PspN4I GGNNCC 1 cut(s) 418
PspPI GGNCC 1 cut(s) 1259
PspPPI RGGWCCY 1 cut(s) 1259
PstI CTGCAG 2 cut(s) 133, 321
RsaI GTAC 4 cut(s) 418, 444, 812, 1027
RsaNI GTAC 4 cut(s) 417, 443, 811, 1026
RseI CAYNNNNRTG 2 cut(s) 61, 910
SalI GTCGAC 1 cut(s) 952
SapI GCTCTTC 2 cut(s) 18, 175
SaqAI TTAA 6 cut(s) 399, 720, 927, 960, 975, 1276
SatI GCNGC 2 cut(s) 320, 348
Sau3AI GATC 3 cut(s) 655, 1051, 1101
Sau96I GGNCC 1 cut(s) 1259
ScaI AGTACT 1 cut(s) 812
SchI GAGTC 6 cut(s) 92, 147, 251, 615, 853, 962
ScrFI CCNGG 3 cut(s) 465, 529, 1196
SduI GDGCHC 1 cut(s) 1194
SfaNI GCATC 1 cut(s) 199
SfcI CTRYAG 2 cut(s) 129, 317
SgrDI CGTCGACG 1 cut(s) 952
SinI GGWCC 1 cut(s) 1259
SmiMI CAYNNNNRTG 2 cut(s) 61, 910
SmlI CTYRAG 3 cut(s) 43, 398, 1263
SmoI CTYRAG 3 cut(s) 43, 398, 1263
Sse9I AATT 3 cut(s) 597, 972, 1015
SsiI CCGC 3 cut(s) 135, 348, 684
SspMI CTAG 4 cut(s) 188, 549, 803, 1244
StyD4I CCNGG 3 cut(s) 463, 527, 1194
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 3 cut(s) 239, 424, 958
TaiI ACGT 2 cut(s) 345, 1134
TaqI TCGA 4 cut(s) 162, 438, 672, 953
TaqII GACCGA 1 cut(s) 321
TasI AATT 3 cut(s) 597, 972, 1015
TatI WGTACW 2 cut(s) 810, 1025
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 3 cut(s) 289, 517, 863
Tru1I TTAA 6 cut(s) 399, 720, 927, 960, 975, 1276
Tru9I TTAA 6 cut(s) 399, 720, 927, 960, 975, 1276
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 1 cut(s) 319
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 6 cut(s) 148, 420, 485, 655, 805, 1112
TspGWI ACGGA 1 cut(s) 663
TspRI CASTG 1 cut(s) 427
Van91I CCANNNNNTGG 2 cut(s) 121, 426
Vha464I CTTAAG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 1259
VspI ATTAAT 2 cut(s) 720, 975
XmiI GTMKAC 1 cut(s) 953
XspI CTAG 4 cut(s) 188, 549, 803, 1244
ZrmI AGTACT 1 cut(s) 812
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.