RLG00000011509

Domain of unknown function (DUF3403)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000509
Physical Location & Seq
Forward (+)
10032836 .. 10036999
4164 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011509

Sequence Viewer

Length: 1524 bp
ATGGCCAGCATCAAAAGAGAATTTCTTCATATTGTTTGTTTGATATCATCCTATGCTTTCTTTTCATTTTCATCTCATGCATTAACTGATACACTTACACCTGAAGAAACACTAACAAACAATCAAACCTTGGTTTCAGCCGGTAAGATTTTTGAGCTTGGCTTCTTCAGTGATGACTTTTCAGGCAATCATTATATTGGCATTTGGTTCAAAGCCGATAAGAACAAGGTTGTCTGGGTTGGTAACCGCGAAATTCCCATATCAGATTCCTCAGGTATTCTTCAAATACGATCTGGGAATCTGGTTTTCATGGACAGACGTCAAGTTCCTTGGCTACTCAATTCTGGAGATGTTGCCACGGTCATAAACACAACTGCAACACTTCTTGATTCAGGAAATTTTGTCCTTAAAGAAGAATATACAGGTACTGTTCTATGGCAAAGTTTTGATAATCCAACTGATACTTATCTTCCTGGGATGAAACTTGGTTGGTCTGCACTAAACACTAACCAACGAAGCTTTCGCTTTTTTGTTTCTTGGGTAAGCCCCCAAAGCCCTACTCGTGGCCTTTTCACCTTAGCCATGGATAGGATCAACTTTACAAAGATTCAGGGTAGGCTCCTAGTAGAAGGACAAGAAATTGCAGTGAAAAGACTGTCCAAAATTTCCCGCCAAGGGTCAGGGGAATTCAAAAATGAAGTCTCAGTGATTTGTAAGCTCCAACACAGGAACTTGGTTAGGCTTTTGGGATGCTGCATTGAAGCAGAAGAAAGTATACTAATTTACGAATACATGCCCAACAAAAGCCTGGACTTTTTCATTTTTGATTCAACCAAACGGTTACTTTTGGACTGGAGAAAGCGTGTGAACATTGTGGAAGGGATTGCTCAAGGCCTTCTATATCTTCACAAATACTCCCGATTAAGGATCATTCACCGTGATTTAAAGACCAGCAATATCCTGTTGGACAGTGACATGAACCCAAAGATTTCTGATTTTGGCATGGCAAGGATTTTCGGGGACAATGAGACTAGAGGCAAAACAAACCGGGTTGTTGGTACATTTGGTTACATGTCACCGGAGTATGCCATGGACGGCCTCTTTTCTGAAAAATCTGATGTGTTTAGCTTTGGGGTGATCCTATTAGAGATTATTACTGGCAAGAAGAATATTGCCTTCTTTGAAGCTGATCATTCTCTGAATTTACTGGGCAAAGCTTGGACTTTGTGGAAAGAAGGTAATGGCATGGAGTTGATGGATTCGACACTGCGTGCTTCTTGTTCGAGCAGTGAAGTTATGAGATACATACAGATGGGTCTTCTGTGCGTGCAAGAAAGAGCTATTGATCGACCAGACATGTCTGATGTAGTTTCTCTGCTAAGCAATCAGTCATTTGCTCTGCCTTCTCCTAAAGAACCTGCATTTTTGAGTCAGCAAATTTCGACTGATGCAGATTCATCTTCAAGTAGGCAAAGACATTACTCTCAGAATGATCTAACCATTTCACAAGTGCATGGCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

508

Amino Acids

57.37

Weight (kDa)

7.12

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 77 - 180 5.5e-27 D-mannose binding lectin
PK_Tyr_Ser-Thr PF07714 210 - 459 3.4e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 210 - 390 7.1e-39 Protein kinase domain
DUF3403 PF11883 466 - 507 2.5e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000509)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29542 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29544 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29560
malus_domestica MD15G1121300.v1.1
prunus_persica Prupe.1G473900_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474100_v2.0.a1 Prupe.1G474300_v2.0.a1 Prupe.1G474500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298411 RchiOBHm_Chr6g0298421 RchiOBHm_Chr6g0298431 RchiOBHm_Chr6g0298441 RchiOBHm_Chr6g0298451 RchiOBHm_Chr6g0298461 RchiOBHm_Chr6g0298471 RchiOBHm_Chr6g0298481 RchiOBHm_Chr6g0298491 RchiOBHm_Chr6g0298501 RchiOBHm_Chr6g0298521
rosa_laevigata RLG00000011506 RLG00000011508 RLG00000011509 RLG00000011511 RLG00000011513 RLG00000011514 RLG00000011515
rosa_multiflora Rmu_co8314937.1_g000001 Rmu_co8319199.1_g000001 Rmu_sc0004500.1_g000002 Rmu_sc0006543.1_g000001 Rmu_ssc0000289.1_g000039 Rmu_ssc0000289.1_g000040 Rmu_ssc0000289.1_g000047 Rmu_ssc0000289.1_g000048 Rmu_ssc0000289.1_g000049 Rmu_ssc0000289.1_g000051
rosa_roxburghii Rroxscaffold_7G00169570 Rroxscaffold_7G00169580 Rroxscaffold_7G00169600 Rroxscaffold_7G00169610 Rroxscaffold_7G00169620 Rroxscaffold_7G00169630 Rroxscaffold_7G00169650 Rroxscaffold_7G00169660 Rroxscaffold_7G00169670
rosa_rugosa Rorug06G0280700 Rorug06G0280800 Rorug06G0280900
rosa_samantha Rh6AG391700 Rh6AG391800 Rh6AG392000 Rh6AG392100 Rh6AG392200 Rh6BG399300 Rh6BG399400 Rh6BG399500 Rh6BG399700 Rh6BG400000 Rh6CG405400 Rh6CG405500 Rh6CG405600 Rh6CG405700 Rh6CG405800 Rh6DG391800 Rh6DG391900 Rh6DG392000 Rh6DG392100 Rh6DG392300
rosa_wichuraiana Rw6G034230 Rw6G034240 Rw6G034250 Rw6G034260 Rw6G034270 Rw6G034280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 322
Acc36I ACCTGC 2 cut(s) 1426, 1509
AccI GTMKAC 1 cut(s) 775
AccII CGCG 1 cut(s) 249
AciI CCGC 2 cut(s) 247, 670
AclWI GGATC 3 cut(s) 599, 935, 1132
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 7 cut(s) 20, 252, 397, 663, 686, 1201, 1437
AcuI CTGAAG 2 cut(s) 123, 151
AcyI GRCGYC 1 cut(s) 319
AdeI CACNNNGTG 1 cut(s) 1271
AfaI GTAC 2 cut(s) 427, 1060
AfiI CCNNNNNNNGG 4 cut(s) 563, 588, 675, 924
AflIII ACRYGT 2 cut(s) 1071, 1356
AgsI TTSAA 7 cut(s) 211, 284, 691, 761, 831, 1184, 1464
AjnI CCWGG 2 cut(s) 472, 807
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
AluBI AGCT 7 cut(s) 157, 519, 718, 1128, 1187, 1217, 1340
AluI AGCT 7 cut(s) 157, 519, 718, 1128, 1187, 1217, 1340
Alw26I GTCTC 2 cut(s) 706, 1022
AlwI GGATC 3 cut(s) 599, 935, 1132
AlwNI CAGNNNCTG 1 cut(s) 428
AoxI GGCC 4 cut(s) 3, 565, 892, 1096
ApeKI GCWGC 1 cut(s) 753
ApoI RAATTY 7 cut(s) 20, 252, 397, 663, 686, 1201, 1437
ArsI GACNNNNNNTTYG 2 cut(s) 684, 716
Asp700I GAANNNNTTC 1 cut(s) 24
AsuC2I CCSGG 1 cut(s) 1049
AsuHPI GGTGA 4 cut(s) 565, 926, 1068, 1147
AxyI CCTNAGG 1 cut(s) 271
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
BauI CACGAG 1 cut(s) 561
BbsI GAAGAC 1 cut(s) 1310
BbvI GCAGC 1 cut(s) 740
BccI CCATC 2 cut(s) 1249, 1306
BceAI ACGGC 1 cut(s) 1111
BciT130I CCWGG 2 cut(s) 474, 809
BclI TGATCA 1 cut(s) 1189
BcnI CCSGG 1 cut(s) 1049
BcoDI GTCTC 2 cut(s) 706, 1022
BfaI CTAG 2 cut(s) 623, 1032
BfuAI ACCTGC 2 cut(s) 1426, 1509
BisI GCNGC 1 cut(s) 754
BlpI GCTNAGC 1 cut(s) 1379
BlsI GCNGC 1 cut(s) 755
Bme1390I CCNGG 3 cut(s) 474, 809, 1049
BmiI GGNNCC 1 cut(s) 620
BmrFI CCNGG 3 cut(s) 474, 809, 1049
BmrI ACTGGG 1 cut(s) 1217
BmsI GCATC 3 cut(s) 18, 740, 1438
BmuI ACTGGG 1 cut(s) 1217
BoxI GACNNNNGTC 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 1310
BpmI CTGGAG 2 cut(s) 366, 874
Bpu10I CCTNAGC 1 cut(s) 577
Bpu1102I GCTNAGC 1 cut(s) 1379
BpuEI CTTGAG 1 cut(s) 873
BpuMI CCSGG 1 cut(s) 1049
BsaBI GATNNNNATC 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 319
BsaJI CCNNGG 7 cut(s) 129, 329, 357, 473, 582, 673, 1089
BsaWI WCCGGW 1 cut(s) 1078
BsaXI ACNNNNNCTCC 4 cut(s) 847, 877, 899, 929
Bsc4I CCNNNNNNNGG 4 cut(s) 563, 588, 675, 924
Bse118I RCCGGY 1 cut(s) 140
Bse1I ACTGG 3 cut(s) 857, 1162, 1212
Bse21I CCTNAGG 1 cut(s) 271
Bse8I GATNNNNATC 1 cut(s) 465
BseBI CCWGG 2 cut(s) 474, 809
BseDI CCNNGG 7 cut(s) 129, 329, 357, 473, 582, 673, 1089
BseGI GGATG 3 cut(s) 47, 483, 755
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 4 cut(s) 563, 588, 675, 924
BseMII CTCAG 3 cut(s) 285, 717, 1499
BseNI ACTGG 3 cut(s) 857, 1162, 1212
BseXI GCAGC 1 cut(s) 740
BsgI GTGCAG 1 cut(s) 480
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 4 cut(s) 5, 567, 894, 1098
BsiSI CCGG 3 cut(s) 141, 1048, 1079
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 4 cut(s) 563, 588, 675, 924
BsmAI GTCTC 2 cut(s) 706, 1022
BsmFI GGGAC 1 cut(s) 1034
BsnI GGCC 4 cut(s) 5, 567, 894, 1098
Bsp143I GATC 7 cut(s) 290, 591, 927, 1137, 1189, 1345, 1492
Bsp1720I GCTNAGC 1 cut(s) 1379
Bsp19I CCATGG 2 cut(s) 582, 1089
BspACI CCGC 2 cut(s) 247, 670
BspANI GGCC 4 cut(s) 5, 567, 894, 1098
BspCNI CTCAG 3 cut(s) 284, 716, 1498
BspFNI CGCG 1 cut(s) 249
BspLI GGNNCC 1 cut(s) 620
BspMI ACCTGC 2 cut(s) 1426, 1509
BspPI GGATC 3 cut(s) 599, 935, 1132
BsrFI RCCGGY 1 cut(s) 140
BsrI ACTGG 3 cut(s) 857, 1162, 1212
BssAI RCCGGY 1 cut(s) 140
BssECI CCNNGG 7 cut(s) 129, 329, 357, 473, 582, 673, 1089
BssMI GATC 7 cut(s) 290, 591, 927, 1137, 1189, 1345, 1492
BssNAI GTATAC 1 cut(s) 776
BssNI GRCGYC 1 cut(s) 319
BssSI CACGAG 1 cut(s) 561
BssT1I CCWWGG 5 cut(s) 129, 329, 582, 673, 1089
Bst1107I GTATAC 1 cut(s) 776
Bst2BI CACGAG 1 cut(s) 561
Bst2UI CCWGG 2 cut(s) 474, 809
Bst4CI ACNGT 6 cut(s) 361, 430, 657, 840, 938, 971
BstACI GRCGYC 1 cut(s) 319
BstC8I GCNNGC 3 cut(s) 7, 1272, 1328
BstDEI CTNAG 5 cut(s) 271, 577, 703, 1379, 1485
BstDSI CCRYGG 3 cut(s) 357, 582, 1089
BstEII GGTNACC 1 cut(s) 242
BstF5I GGATG 3 cut(s) 47, 483, 755
BstFNI CGCG 1 cut(s) 249
BstKTI GATC 7 cut(s) 293, 594, 930, 1140, 1192, 1348, 1495
BstMAI GTCTC 2 cut(s) 706, 1022
BstMBI GATC 7 cut(s) 290, 591, 927, 1137, 1189, 1345, 1492
BstMWI GCNNNNNNNGC 1 cut(s) 552
BstNI CCWGG 2 cut(s) 474, 809
BstNSI RCATGY 3 cut(s) 796, 1075, 1360
BstPAI GACNNNNGTC 1 cut(s) 318
BstPI GGTNACC 1 cut(s) 242
BstSCI CCNGG 3 cut(s) 472, 807, 1047
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 740
BstV2I GAAGAC 1 cut(s) 1310
BstZ17I GTATAC 1 cut(s) 776
Bsu36I CCTNAGG 1 cut(s) 271
BsuRI GGCC 4 cut(s) 5, 567, 894, 1098
BtgI CCRYGG 3 cut(s) 357, 582, 1089
BtsCI GGATG 3 cut(s) 47, 483, 755
BtsI GCAGTG 3 cut(s) 651, 1265, 1294
BtsIMutI CAGTG 6 cut(s) 175, 651, 711, 976, 1265, 1294
BveI ACCTGC 2 cut(s) 1426, 1509
Cac8I GCNNGC 3 cut(s) 7, 1272, 1328
CaiI CAGNNNCTG 1 cut(s) 428
Cfr10I RCCGGY 1 cut(s) 140
Csp6I GTAC 2 cut(s) 426, 1059
CviQI GTAC 2 cut(s) 426, 1059
DdeI CTNAG 5 cut(s) 271, 577, 703, 1379, 1485
DpnI GATC 7 cut(s) 292, 593, 929, 1139, 1191, 1347, 1494
DpnII GATC 7 cut(s) 290, 591, 927, 1137, 1189, 1345, 1492
DraI TTTAAA 1 cut(s) 945
DraIII CACNNNGTG 1 cut(s) 1271
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 5 cut(s) 129, 329, 582, 673, 1089
Eco147I AGGCCT 1 cut(s) 894
Eco32I GATATC 1 cut(s) 45
Eco57I CTGAAG 2 cut(s) 123, 151
Eco81I CCTNAGG 1 cut(s) 271
Eco91I GGTNACC 1 cut(s) 242
EcoO65I GGTNACC 1 cut(s) 242
EcoRI GAATTC 1 cut(s) 686
EcoRII CCWGG 2 cut(s) 472, 807
EcoRV GATATC 1 cut(s) 45
EcoT14I CCWWGG 5 cut(s) 129, 329, 582, 673, 1089
EcoT22I ATGCAT 1 cut(s) 82
ErhI CCWWGG 5 cut(s) 129, 329, 582, 673, 1089
FaqI GGGAC 1 cut(s) 1034
FauI CCCGC 1 cut(s) 677
FbaI TGATCA 1 cut(s) 1189
FblI GTMKAC 1 cut(s) 775
Fnu4HI GCNGC 1 cut(s) 754
FokI GGATG 3 cut(s) 34, 490, 762
Fsp4HI GCNGC 1 cut(s) 754
FspBI CTAG 2 cut(s) 623, 1032
GluI GCNGC 1 cut(s) 754
GsuI CTGGAG 2 cut(s) 366, 874
HaeIII GGCC 4 cut(s) 5, 567, 894, 1098
HapII CCGG 3 cut(s) 141, 1048, 1079
Hin1I GRCGYC 1 cut(s) 319
HindIII AAGCTT 2 cut(s) 517, 1215
HinfI GANTC 8 cut(s) 266, 298, 389, 607, 827, 1259, 1429, 1454
HpaII CCGG 3 cut(s) 141, 1048, 1079
HphI GGTGA 4 cut(s) 565, 926, 1068, 1147
Hpy166II GTNNAC 2 cut(s) 776, 868
Hpy188I TCNGA 7 cut(s) 265, 994, 1108, 1117, 1200, 1363, 1488
Hpy188III TCNNGA 4 cut(s) 345, 386, 393, 918
Hpy8I GTNNAC 2 cut(s) 776, 868
HpyAV CCTTC 6 cut(s) 623, 872, 905, 1186, 1229, 1413
HpyCH4III ACNGT 6 cut(s) 361, 430, 657, 840, 938, 971
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 9 cut(s) 80, 377, 497, 644, 756, 1330, 1421, 1451, 1513
HpyF10VI GCNNNNNNNGC 1 cut(s) 552
HpyF3I CTNAG 5 cut(s) 271, 577, 703, 1379, 1485
HpySE526I ACGT 1 cut(s) 319
Hsp92I GRCGYC 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 1189
Kzo9I GATC 7 cut(s) 290, 591, 927, 1137, 1189, 1345, 1492
LmnI GCTCC 2 cut(s) 624, 723
Lsp1109I GCAGC 1 cut(s) 740
LweI GCATC 3 cut(s) 18, 740, 1438
MaeI CTAG 2 cut(s) 623, 1032
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 5 cut(s) 242, 840, 971, 1067, 1074
MalI GATC 7 cut(s) 292, 593, 929, 1139, 1191, 1347, 1494
MboI GATC 7 cut(s) 290, 591, 927, 1137, 1189, 1345, 1492
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 1438
MmeI TCCRAC 3 cut(s) 479, 745, 945
MnlI CCTC 3 cut(s) 280, 1028, 1109
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 24
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 83, 408, 923, 944
MslI CAYNNNNRTG 1 cut(s) 1310
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 141, 1048, 1079
MspR9I CCNGG 3 cut(s) 474, 809, 1049
MvaI CCWGG 2 cut(s) 474, 809
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 552
NciI CCSGG 1 cut(s) 1049
NcoI CCATGG 2 cut(s) 582, 1089
NdeII GATC 7 cut(s) 290, 591, 927, 1137, 1189, 1345, 1492
NlaIV GGNNCC 1 cut(s) 620
NmuCI GTSAC 2 cut(s) 971, 1074
NsiI ATGCAT 1 cut(s) 82
NspI RCATGY 3 cut(s) 796, 1075, 1360
PceI AGGCCT 1 cut(s) 894
PciI ACATGT 2 cut(s) 1071, 1356
PdmI GAANNNNTTC 1 cut(s) 24
PfeI GAWTC 7 cut(s) 266, 298, 389, 607, 827, 1259, 1454
PkrI GCNGC 1 cut(s) 755
PleI GAGTC 1 cut(s) 1437
PpsI GAGTC 1 cut(s) 1437
PscI ACATGT 2 cut(s) 1071, 1356
PshAI GACNNNNGTC 1 cut(s) 318
Psp6I CCWGG 2 cut(s) 472, 807
PspEI GGTNACC 1 cut(s) 242
PspGI CCWGG 2 cut(s) 472, 807
PspN4I GGNNCC 1 cut(s) 620
PstNI CAGNNNCTG 1 cut(s) 428
RsaI GTAC 2 cut(s) 427, 1060
RsaNI GTAC 2 cut(s) 426, 1059
RseI CAYNNNNRTG 1 cut(s) 1310
SaqAI TTAA 4 cut(s) 83, 408, 923, 944
SatI GCNGC 1 cut(s) 754
Sau3AI GATC 7 cut(s) 290, 591, 927, 1137, 1189, 1345, 1492
SchI GAGTC 1 cut(s) 1438
ScrFI CCNGG 3 cut(s) 474, 809, 1049
SfaNI GCATC 3 cut(s) 18, 740, 1438
SmiMI CAYNNNNRTG 1 cut(s) 1310
SmlI CTYRAG 1 cut(s) 888
SmoI CTYRAG 1 cut(s) 888
SseBI AGGCCT 1 cut(s) 894
SsiI CCGC 2 cut(s) 247, 670
SspI AATATT 1 cut(s) 1171
SspMI CTAG 2 cut(s) 623, 1032
StuI AGGCCT 1 cut(s) 894
StyD4I CCNGG 3 cut(s) 472, 807, 1047
StyI CCWWGG 5 cut(s) 129, 329, 582, 673, 1089
TaaI ACNGT 6 cut(s) 361, 430, 657, 840, 938, 971
TaiI ACGT 1 cut(s) 322
TaqI TCGA 4 cut(s) 1262, 1283, 1348, 1442
TfiI GAWTC 7 cut(s) 266, 298, 389, 607, 827, 1259, 1454
Tru1I TTAA 4 cut(s) 83, 408, 923, 944
Tru9I TTAA 4 cut(s) 83, 408, 923, 944
TscAI CASTG 6 cut(s) 175, 651, 711, 976, 1272, 1294
TseFI GTSAC 2 cut(s) 971, 1074
TseI GCWGC 1 cut(s) 753
Tsp45I GTSAC 2 cut(s) 971, 1074
TspDTI ATGAA 9 cut(s) 17, 54, 60, 298, 494, 711, 808, 992, 1446
TspRI CASTG 6 cut(s) 175, 651, 711, 976, 1272, 1294
XapI RAATTY 7 cut(s) 20, 252, 397, 663, 686, 1201, 1437
XceI RCATGY 3 cut(s) 796, 1075, 1360
XcmI CCANNNNNNNNNTGG 1 cut(s) 805
XmiI GTMKAC 1 cut(s) 775
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 2 cut(s) 623, 1032
ZraI GACGTC 1 cut(s) 320
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.