RchiOBHm_Chr6g0298521

Domain of unknown function (DUF3403)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000509
Physical Location & Seq
Reverse (-)
59902101 .. 59906637
4537 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26799

Sequence Viewer

Length: 2313 bp
ATGGCAATGGCTCTTCCTCTTATTATTTGCTTGATATTATTCTCAAGCTTGTTTCCATTTCCATGCCGTGCAATCACAGACACGCTCACAGCTTCAGACTCGTTGAGCGACAACCAAACGCTGGTTTCTGCAGGCGGAGTCTTCGTCCTTGGCTTCTTCATCGATAGCTCTTCAGGTAATCACTATCTAGGTATATGGCTGAGAGCTGATGCAAAGAGAGTTGTTTGGGTTGCCTACCGTGAAATACCTATATTGGACTCTTCCGGCGTGCTTCAAATCCGGTCAGGGAATCTGGTTGTCTCCGACCGACGACAACTGCAGCTCATAGTCAACTCGGGAAACGTTGCCACCACCACCAACACGAGTGCAACACTTCTTGATACGGGAAATCTTGTGCTCAAGGATGAAAATACAGGTACCACTGTATGGCAAAGCTTCGATGCACCAACTGATACATATCTTCCGGGGATGAAAGTTGGGTTGTTTGGCCTAAACACAAGCCAACAAAGCTTTCGGATTCTCACTTCCTGGGAAAGCGCTCAAAACCCTAGTCGTGGCCTCTTCACCTTCTCGCTGGATAGAATAGACTTCACTAAAATTGATGTTTGGCGAGGAGATGGACTCCAAATGCATATCGCTTTTTGGGACGGACATGATCTCCGGTTCATTTTCGAGAACACAACCGCAAACAATGATTACAACTTTAGCTACACTTCCATTAATGGAAGTGAAGCCTACTACACTTTTAGCCACAACAAGAAGTATGACCTCATGTGGTTTGTAATGGCTTCAACAGGAAACCTAGACCAGTACTTCATGGTGAATGGGAAGATTACTTCTACGAGTCATGCTTTGTGTGAAGATTCAAGTGGTGGTAATAGTGGAAAGTGCTTGACTTCTTTACCACCTATGTGTGGTAATACTTTTGACCAGACGAATGGTTCGTTGCCGTCGACGGTTAAGGTTGACTCAATTAATATAGGGGCTAATGATTGTGAGACTTTGTGCAAGAGCAATTGTTCTTGTACTGCATATGTTTCAGTTCAGAATGATCAATCGGTTTGTCAGCTTTATTATGGGAGTAAACAGGAGTTGATGAAGATGATGGAGATAGGGCCAGGGACTATCAACGTGCGTGTTTCTGCTAATACAAGTGGAATTAGTAGGCGAGAAGGGATTATCATTTCGGATGAAGTGAGATTATTCCAAATTGGCTCCACCAATGCCTCACCAATTCAGTATGATCTAGCTAGGGTTGCCAATATGATGGAATTGAGCAGATCACAGAAGGATCAAGAACTGCCATTCTTTACTTTCTCTGCCATACAAACTGCAACAAATGACTTTGCAAAGGCTAATAAACTAGGGGAAGGTGGATTTGGGCCTGTCTACAAGGGGAAGCTATTAGAAGGACAGGAAATTGCAGTGAAAAGGTTGTCTGAACTGTCGAGGCAAGGGTTGGAGGAGTTCAAAAACGAGGTCTTAGTCATTTGTAAGCTCCAACACAGGAACCTGGTTAGGATCTTGGGGTGCTGCATTGAAGGAGAAGAAAGTATACTAGTTTATGAGTACATGCCCAACAAAAGCTTAGATTCCTTCATTTTTGATCCAGCCAAACGGGTGCTTTTGGATTGGAAAAAGCGCATGAACATTATTGATGGGATTTCTCAAGGCCTTCTATATCTTCACAAATACTCCAGATTACGGATCATTCACCGCGATCTAAAGACCAGCAATATTTTGCTAGACAGCGACATGAACCCCAAAATTTCTGATTTTGGCATGGCAAGGATTTTTGGGGACAATGACACTAGAGGAAAAACAAACCGTGTTGTTGGTACATTTGGTTACATGTCTCCGGAGTATGCCATGGGCGGCCTATTTTCTGAAAAATCAGATGTGTTTAGCTTCGGGGTGATCTTATTAGAGGTCATTAGTGGGAAGAAGAACATTCCATTTTTTGAGTCTGATCAAGCTAGTCTAAACTTACTTGGCAATGCCTGGAATTTATGGAATGAAGGCAAGAGCATGGAGTTACTGGATTCAGCACTGGTTGCATCTTGTTCCAGCAGTGAAGTTATGAGGTGCATTCAGATGGGTCTTTTGTGCGTGCAAGAAAGAGCCATGGATCGACCTAATATGTCGGATGTGGCTTCGATGCTAAGCAATCAATCAATTCCTCTGCCTCTTCCAAAAGAACCTGCATTTTGGAGCCAGCTAAGTTCTACTGATGCAGATACATCTTCAAGCAGGCAAAGACATTACTCTACAATTGAGTCAACTCTAACTATTTCAGAAGTGCATGCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

770

Amino Acids

85.44

Weight (kDa)

5.49

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 74 - 177 8.1e-27 D-mannose binding lectin
Pkinase PF00069 450 - 658 6.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 452 - 720 9e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000509)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29542 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29544 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29560
malus_domestica MD15G1121300.v1.1
prunus_persica Prupe.1G473900_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474100_v2.0.a1 Prupe.1G474300_v2.0.a1 Prupe.1G474500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298411 RchiOBHm_Chr6g0298421 RchiOBHm_Chr6g0298431 RchiOBHm_Chr6g0298441 RchiOBHm_Chr6g0298451 RchiOBHm_Chr6g0298461 RchiOBHm_Chr6g0298471 RchiOBHm_Chr6g0298481 RchiOBHm_Chr6g0298491 RchiOBHm_Chr6g0298501 RchiOBHm_Chr6g0298521
rosa_laevigata RLG00000011506 RLG00000011508 RLG00000011509 RLG00000011511 RLG00000011513 RLG00000011514 RLG00000011515
rosa_multiflora Rmu_co8314937.1_g000001 Rmu_co8319199.1_g000001 Rmu_sc0004500.1_g000002 Rmu_sc0006543.1_g000001 Rmu_ssc0000289.1_g000039 Rmu_ssc0000289.1_g000040 Rmu_ssc0000289.1_g000047 Rmu_ssc0000289.1_g000048 Rmu_ssc0000289.1_g000049 Rmu_ssc0000289.1_g000051
rosa_roxburghii Rroxscaffold_7G00169570 Rroxscaffold_7G00169580 Rroxscaffold_7G00169600 Rroxscaffold_7G00169610 Rroxscaffold_7G00169620 Rroxscaffold_7G00169630 Rroxscaffold_7G00169650 Rroxscaffold_7G00169660 Rroxscaffold_7G00169670
rosa_rugosa Rorug06G0280700 Rorug06G0280800 Rorug06G0280900
rosa_samantha Rh6AG391700 Rh6AG391800 Rh6AG392000 Rh6AG392100 Rh6AG392200 Rh6BG399300 Rh6BG399400 Rh6BG399500 Rh6BG399700 Rh6BG400000 Rh6CG405400 Rh6CG405500 Rh6CG405600 Rh6CG405700 Rh6CG405800 Rh6DG391800 Rh6DG391900 Rh6DG392000 Rh6DG392100 Rh6DG392300
rosa_wichuraiana Rw6G034230 Rw6G034240 Rw6G034250 Rw6G034260 Rw6G034270 Rw6G034280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2209
Acc65I GGTACC 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 416
AccB7I CCANNNNNTGG 2 cut(s) 121, 426
AccI GTMKAC 3 cut(s) 953, 1389, 1555
AccII CGCG 1 cut(s) 1719
AccIII TCCGGA 1 cut(s) 1858
AciI CCGC 4 cut(s) 135, 684, 1717, 1875
AclI AACGTT 1 cut(s) 342
AclWI GGATC 5 cut(s) 1299, 1529, 1601, 1715, 2136
AcsI RAATTY 2 cut(s) 1767, 2005
AcuI CTGAAG 2 cut(s) 78, 156
AfaI GTAC 5 cut(s) 418, 812, 1027, 1571, 1840
AfeI AGCGCT 1 cut(s) 538
AfiI CCNNNNNNNGG 5 cut(s) 121, 426, 554, 914, 1704
AflIII ACRYGT 1 cut(s) 1851
AgsI TTSAA 6 cut(s) 275, 792, 867, 1471, 1541, 2247
AhlI ACTAGT 1 cut(s) 1558
AjnI CCWGG 4 cut(s) 527, 1117, 1512, 2000
AjuI GAANNNNNNNTTGG 4 cut(s) 495, 527, 1362, 1394
AleI CACNNNNGTG 1 cut(s) 910
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 3 cut(s) 304, 992, 1860
AlwI GGATC 5 cut(s) 1299, 1529, 1601, 1715, 2136
Ama87I CYCGRG 1 cut(s) 334
Aor13HI TCCGGA 1 cut(s) 1858
Aor51HI AGCGCT 1 cut(s) 538
AoxI GGCC 6 cut(s) 487, 556, 1115, 1382, 1672, 1876
ApeKI GCWGC 2 cut(s) 319, 1533
ApoI RAATTY 2 cut(s) 1767, 2005
ArsI GACNNNNNNTTYG 6 cut(s) 535, 567, 925, 957, 1464, 1496
AseI ATTAAT 2 cut(s) 720, 975
Asp718I GGTACC 1 cut(s) 416
AspLEI GCGC 2 cut(s) 539, 1644
AspS9I GGNCC 2 cut(s) 1115, 1382
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 5 cut(s) 556, 832, 1221, 1706, 1927
AvaI CYCGRG 1 cut(s) 334
BaeI ACNNNNGTAYC 2 cut(s) 408, 441
BanI GGYRCC 1 cut(s) 416
BarI GAAGNNNNNNTAC 2 cut(s) 1539, 1571
BauI CACGAG 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 2 cut(s) 331, 1520
BccI CCATC 5 cut(s) 611, 1099, 1261, 1652, 2089
BceAI ACGGC 2 cut(s) 51, 934
BciT130I CCWGG 4 cut(s) 529, 1119, 1514, 2002
BclI TGATCA 2 cut(s) 1051, 1969
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 3 cut(s) 304, 992, 1860
BcuI ACTAGT 1 cut(s) 1558
BfmI CTRYAG 2 cut(s) 129, 317
BfoI RGCGCY 1 cut(s) 540
BfuAI ACCTGC 1 cut(s) 2209
BisI GCNGC 3 cut(s) 320, 1534, 1876
BlpI GCTNAGC 1 cut(s) 2162
BlsI GCNGC 3 cut(s) 321, 1535, 1877
BmcAI AGTACT 1 cut(s) 812
Bme1390I CCNGG 5 cut(s) 465, 529, 1119, 1514, 2002
BmeT110I CYCGRG 1 cut(s) 334
BmgT120I GGNCC 2 cut(s) 1115, 1382
BmiI GGNNCC 4 cut(s) 418, 1216, 1511, 2213
BmrFI CCNGG 5 cut(s) 465, 529, 1119, 1514, 2002
BmsI GCATC 5 cut(s) 199, 430, 2066, 2148, 2221
BpiI GAAGAC 1 cut(s) 133
BplI GAGNNNNNCTC 2 cut(s) 606, 638
BpmI CTGGAG 1 cut(s) 1681
Bpu1102I GCTNAGC 1 cut(s) 2162
BpuEI CTTGAG 3 cut(s) 28, 383, 1653
BpuMI CCSGG 1 cut(s) 465
Bsa29I ATCGAT 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 456
BsaJI CCNNGG 6 cut(s) 148, 464, 528, 1118, 1869, 2124
BsaWI WCCGGW 3 cut(s) 279, 660, 1858
BsaXI ACNNNNNCTCC 6 cut(s) 129, 159, 642, 672, 1679, 1709
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 426, 554, 914, 1704
Bse1I ACTGG 3 cut(s) 808, 2043, 2055
Bse3DI GCAATG 2 cut(s) 12, 2002
Bse8I GATNNNNATC 1 cut(s) 456
BseAI TCCGGA 1 cut(s) 1858
BseBI CCWGG 4 cut(s) 529, 1119, 1514, 2002
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 6 cut(s) 148, 464, 528, 1118, 1869, 2124
BseGI GGATG 4 cut(s) 409, 474, 1195, 2152
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 5 cut(s) 121, 426, 554, 914, 1704
BseMI GCAATG 2 cut(s) 12, 2002
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 3 cut(s) 808, 2043, 2055
BseRI GAGGAG 2 cut(s) 627, 1478
BseXI GCAGC 2 cut(s) 331, 1520
Bsh1236I CGCG 1 cut(s) 1719
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 6 cut(s) 489, 558, 1117, 1384, 1674, 1878
BshNI GGYRCC 1 cut(s) 416
BshVI ATCGAT 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 399
BsiHKCI CYCGRG 1 cut(s) 334
BsiSI CCGG 5 cut(s) 264, 280, 464, 661, 1859
BslFI GGGAC 3 cut(s) 659, 1135, 1814
BslI CCNNNNNNNGG 5 cut(s) 121, 426, 554, 914, 1704
BsmAI GTCTC 3 cut(s) 304, 992, 1860
BsmFI GGGAC 3 cut(s) 659, 1135, 1814
BsmI GAATGC 1 cut(s) 2088
BsnI GGCC 6 cut(s) 489, 558, 1117, 1384, 1674, 1878
BsoBI CYCGRG 1 cut(s) 334
Bsp1286I GDGCHC 1 cut(s) 399
Bsp13I TCCGGA 1 cut(s) 1858
Bsp1720I GCTNAGC 1 cut(s) 2162
Bsp19I CCATGG 2 cut(s) 1869, 2124
BspACI CCGC 4 cut(s) 135, 684, 1717, 1875
BspANI GGCC 6 cut(s) 489, 558, 1117, 1384, 1674, 1878
BspCNI CTCAG 1 cut(s) 192
BspDI ATCGAT 1 cut(s) 162
BspEI TCCGGA 1 cut(s) 1858
BspFNI CGCG 1 cut(s) 1719
BspLI GGNNCC 4 cut(s) 418, 1216, 1511, 2213
BspMAI CTGCAG 2 cut(s) 133, 321
BspMI ACCTGC 1 cut(s) 2209
BspPI GGATC 5 cut(s) 1299, 1529, 1601, 1715, 2136
BspQI GCTCTTC 2 cut(s) 18, 175
BspT107I GGYRCC 1 cut(s) 416
BsrDI GCAATG 2 cut(s) 12, 2002
BsrI ACTGG 3 cut(s) 808, 2043, 2055
BssECI CCNNGG 6 cut(s) 148, 464, 528, 1118, 1869, 2124
BssNAI GTATAC 1 cut(s) 1556
BssSI CACGAG 1 cut(s) 361
BssT1I CCWWGG 3 cut(s) 148, 1869, 2124
Bst1107I GTATAC 1 cut(s) 1556
Bst2BI CACGAG 1 cut(s) 361
Bst2UI CCWGG 4 cut(s) 529, 1119, 1514, 2002
Bst4CI ACNGT 5 cut(s) 239, 424, 958, 1446, 1829
Bst6I CTCTTC 5 cut(s) 18, 175, 265, 566, 2193
BstAPI GCANNNNNTGC 1 cut(s) 2054
BstC8I GCNNGC 6 cut(s) 133, 269, 2111, 2216, 2252, 2304
BstDEI CTNAG 5 cut(s) 200, 1483, 1588, 2162, 2219
BstDSI CCRYGG 2 cut(s) 1869, 2124
BstF5I GGATG 4 cut(s) 409, 474, 1195, 2152
BstFNI CGCG 1 cut(s) 1719
BstH2I RGCGCY 1 cut(s) 540
BstHHI GCGC 2 cut(s) 539, 1644
BstMAI GTCTC 3 cut(s) 304, 992, 1860
BstMCI CGRYCG 1 cut(s) 307
BstMWI GCNNNNNNNGC 3 cut(s) 507, 1256, 2054
BstNI CCWGG 4 cut(s) 529, 1119, 1514, 2002
BstNSI RCATGY 3 cut(s) 1576, 1855, 2306
BstSCI CCNGG 5 cut(s) 463, 527, 1117, 1512, 2000
BstSFI CTRYAG 2 cut(s) 129, 317
BstUI CGCG 1 cut(s) 1719
BstV1I GCAGC 2 cut(s) 331, 1520
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 1521
BstXI CCANNNNNNTGG 2 cut(s) 938, 1267
BstYI RGATCY 1 cut(s) 1521
BstZ17I GTATAC 1 cut(s) 1556
Bsu15I ATCGAT 1 cut(s) 162
BsuRI GGCC 6 cut(s) 489, 558, 1117, 1384, 1674, 1878
BsuTUI ATCGAT 1 cut(s) 162
BtgI CCRYGG 2 cut(s) 1869, 2124
BtsCI GGATG 4 cut(s) 409, 474, 1195, 2152
BtsI GCAGTG 2 cut(s) 1431, 2077
BtsIMutI CAGTG 4 cut(s) 420, 1431, 2048, 2077
BveI ACCTGC 1 cut(s) 2209
Cac8I GCNNGC 6 cut(s) 133, 269, 2111, 2216, 2252, 2304
CfoI GCGC 2 cut(s) 539, 1644
Cfr13I GGNCC 2 cut(s) 1115, 1382
ClaI ATCGAT 1 cut(s) 162
CsiI ACCWGGT 1 cut(s) 1512
Csp6I GTAC 5 cut(s) 417, 811, 1026, 1570, 1839
CviQI GTAC 5 cut(s) 417, 811, 1026, 1570, 1839
DdeI CTNAG 5 cut(s) 200, 1483, 1588, 2162, 2219
Eam1104I CTCTTC 5 cut(s) 18, 175, 265, 566, 2193
EarI CTCTTC 5 cut(s) 18, 175, 265, 566, 2193
EciI GGCGGA 1 cut(s) 150
Eco130I CCWWGG 3 cut(s) 148, 1869, 2124
Eco147I AGGCCT 1 cut(s) 1674
Eco47III AGCGCT 1 cut(s) 538
Eco57I CTGAAG 2 cut(s) 78, 156
Eco88I CYCGRG 1 cut(s) 334
EcoRII CCWGG 4 cut(s) 527, 1117, 1512, 2000
EcoT14I CCWWGG 3 cut(s) 148, 1869, 2124
EcoT22I ATGCAT 1 cut(s) 633
ErhI CCWWGG 3 cut(s) 148, 1869, 2124
FaqI GGGAC 3 cut(s) 659, 1135, 1814
FauNDI CATATG 1 cut(s) 1033
FbaI TGATCA 2 cut(s) 1051, 1969
FblI GTMKAC 3 cut(s) 953, 1389, 1555
Fnu4HI GCNGC 3 cut(s) 320, 1534, 1876
FokI GGATG 4 cut(s) 416, 481, 1202, 2159
Fsp4HI GCNGC 3 cut(s) 320, 1534, 1876
GlaI GCGC 2 cut(s) 538, 1643
GluI GCNGC 3 cut(s) 320, 1534, 1876
GsuI CTGGAG 1 cut(s) 1681
HaeII RGCGCY 1 cut(s) 540
HaeIII GGCC 6 cut(s) 489, 558, 1117, 1384, 1674, 1878
HapII CCGG 5 cut(s) 264, 280, 464, 661, 1859
HhaI GCGC 2 cut(s) 539, 1644
Hin6I GCGC 2 cut(s) 537, 1642
HinP1I GCGC 2 cut(s) 537, 1642
HincII GTYRAC 4 cut(s) 331, 954, 967, 2280
HindII GTYRAC 4 cut(s) 331, 954, 967, 2280
HindIII AAGCTT 4 cut(s) 46, 433, 508, 1585
HpaII CCGG 5 cut(s) 264, 280, 464, 661, 1859
HphI GGTGA 5 cut(s) 556, 832, 1221, 1706, 1927
Hpy166II GTNNAC 7 cut(s) 331, 954, 967, 1085, 1390, 1556, 2280
Hpy188III TCNNGA 6 cut(s) 336, 377, 673, 1295, 1698, 1859
Hpy8I GTNNAC 7 cut(s) 331, 954, 967, 1085, 1390, 1556, 2280
Hpy99I CGWCG 3 cut(s) 312, 955, 958
HpyAV CCTTC 9 cut(s) 577, 1166, 1282, 1364, 1403, 1535, 1606, 1685, 2012
HpyCH4III ACNGT 5 cut(s) 239, 424, 958, 1446, 1829
HpyCH4IV ACGT 2 cut(s) 342, 1131
HpyF10VI GCNNNNNNNGC 3 cut(s) 507, 1256, 2054
HpyF3I CTNAG 5 cut(s) 200, 1483, 1588, 2162, 2219
HpySE526I ACGT 2 cut(s) 342, 1131
HspAI GCGC 2 cut(s) 537, 1642
Kpn2I TCCGGA 1 cut(s) 1858
KpnI GGTACC 1 cut(s) 420
Ksp22I TGATCA 2 cut(s) 1051, 1969
LguI GCTCTTC 2 cut(s) 18, 175
LmnI GCTCC 3 cut(s) 1220, 1503, 2211
Lsp1109I GCAGC 2 cut(s) 331, 1520
LweI GCATC 5 cut(s) 199, 430, 2066, 2148, 2221
MabI ACCWGGT 1 cut(s) 1512
MaeII ACGT 2 cut(s) 342, 1131
MaeIII GTNAC 2 cut(s) 1847, 2034
MfeI CAATTG 2 cut(s) 1015, 2271
MflI RGATCY 1 cut(s) 1521
MhlI GDGCHC 1 cut(s) 399
MlyI GAGTC 8 cut(s) 92, 147, 251, 615, 853, 962, 1973, 2285
MmeI TCCRAC 4 cut(s) 327, 1440, 1525, 2124
Mph1103I ATGCAT 1 cut(s) 633
MroI TCCGGA 1 cut(s) 1858
MseI TTAA 3 cut(s) 720, 960, 975
MslI CAYNNNNRTG 3 cut(s) 61, 910, 2093
MspI CCGG 5 cut(s) 264, 280, 464, 661, 1859
MspR9I CCNGG 5 cut(s) 465, 529, 1119, 1514, 2002
MunI CAATTG 2 cut(s) 1015, 2271
Mva1269I GAATGC 1 cut(s) 2088
MvaI CCWGG 4 cut(s) 529, 1119, 1514, 2002
MvnI CGCG 1 cut(s) 1719
MwoI GCNNNNNNNGC 3 cut(s) 507, 1256, 2054
NciI CCSGG 1 cut(s) 465
NcoI CCATGG 2 cut(s) 1869, 2124
NdeI CATATG 1 cut(s) 1033
NlaIV GGNNCC 4 cut(s) 418, 1216, 1511, 2213
NsiI ATGCAT 1 cut(s) 633
NspI RCATGY 3 cut(s) 1576, 1855, 2306
OliI CACNNNNGTG 1 cut(s) 910
PaeI GCATGC 1 cut(s) 2306
PceI AGGCCT 1 cut(s) 1674
PciI ACATGT 1 cut(s) 1851
PciSI GCTCTTC 2 cut(s) 18, 175
PcsI WCGNNNNNNNCGW 2 cut(s) 941, 950
PctI GAATGC 1 cut(s) 2088
PfeI GAWTC 5 cut(s) 289, 517, 863, 1592, 2042
PflMI CCANNNNNTGG 2 cut(s) 121, 426
PkrI GCNGC 3 cut(s) 321, 1535, 1877
PleI GAGTC 8 cut(s) 92, 146, 251, 615, 852, 962, 1972, 2284
PpsI GAGTC 8 cut(s) 92, 146, 251, 615, 852, 962, 1972, 2284
PscI ACATGT 1 cut(s) 1851
PshBI ATTAAT 2 cut(s) 720, 975
Psp1406I AACGTT 1 cut(s) 342
Psp6I CCWGG 4 cut(s) 527, 1117, 1512, 2000
PspGI CCWGG 4 cut(s) 527, 1117, 1512, 2000
PspN4I GGNNCC 4 cut(s) 418, 1216, 1511, 2213
PspPI GGNCC 2 cut(s) 1115, 1382
PstI CTGCAG 2 cut(s) 133, 321
PsuI RGATCY 1 cut(s) 1521
RsaI GTAC 5 cut(s) 418, 812, 1027, 1571, 1840
RsaNI GTAC 5 cut(s) 417, 811, 1026, 1570, 1839
RseI CAYNNNNRTG 3 cut(s) 61, 910, 2093
SalI GTCGAC 1 cut(s) 952
SapI GCTCTTC 2 cut(s) 18, 175
SaqAI TTAA 3 cut(s) 720, 960, 975
SatI GCNGC 3 cut(s) 320, 1534, 1876
Sau96I GGNCC 2 cut(s) 1115, 1382
ScaI AGTACT 1 cut(s) 812
SchI GAGTC 8 cut(s) 92, 147, 251, 615, 853, 962, 1973, 2285
ScrFI CCNGG 5 cut(s) 465, 529, 1119, 1514, 2002
SduI GDGCHC 1 cut(s) 399
SexAI ACCWGGT 1 cut(s) 1512
SfaNI GCATC 5 cut(s) 199, 430, 2066, 2148, 2221
SfcI CTRYAG 2 cut(s) 129, 317
SgrDI CGTCGACG 1 cut(s) 952
SmiMI CAYNNNNRTG 3 cut(s) 61, 910, 2093
SmlI CTYRAG 3 cut(s) 43, 398, 1668
SmoI CTYRAG 3 cut(s) 43, 398, 1668
SpeI ACTAGT 1 cut(s) 1558
SphI GCATGC 1 cut(s) 2306
SseBI AGGCCT 1 cut(s) 1674
SsiI CCGC 4 cut(s) 135, 684, 1717, 1875
SspI AATATT 1 cut(s) 1738
StuI AGGCCT 1 cut(s) 1674
StyD4I CCNGG 5 cut(s) 463, 527, 1117, 1512, 2000
StyI CCWWGG 3 cut(s) 148, 1869, 2124
TaaI ACNGT 5 cut(s) 239, 424, 958, 1446, 1829
TaiI ACGT 2 cut(s) 345, 1134
TaqI TCGA 7 cut(s) 162, 438, 672, 953, 1448, 2131, 2156
TaqII GACCGA 1 cut(s) 321
TatI WGTACW 3 cut(s) 810, 1025, 1569
TauI GCSGC 1 cut(s) 1878
TfiI GAWTC 5 cut(s) 289, 517, 863, 1592, 2042
Tru1I TTAA 3 cut(s) 720, 960, 975
Tru9I TTAA 3 cut(s) 720, 960, 975
TscAI CASTG 4 cut(s) 427, 1431, 2055, 2077
TseI GCWGC 2 cut(s) 319, 1533
TspGWI ACGGA 2 cut(s) 663, 1720
TspRI CASTG 4 cut(s) 427, 1431, 2055, 2077
Van91I CCANNNNNTGG 2 cut(s) 121, 426
VspI ATTAAT 2 cut(s) 720, 975
XapI RAATTY 2 cut(s) 1767, 2005
XceI RCATGY 3 cut(s) 1576, 1855, 2306
XmiI GTMKAC 3 cut(s) 953, 1389, 1555
ZrmI AGTACT 1 cut(s) 812
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.