MD15G1121300.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000509
Physical Location & Seq
Forward (+)
8711261 .. 8715823
4563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1121300.v1.1.491

Sequence Viewer

Length: 2427 bp
ATGGCCATGTTTCTTCCTCTTACAATTTTCTTGATCTCTTTCTCTTCCTTCTCTTCATCTCCATCTCTTGCAAAAGACACACTCACACCTGCAGACATGCTGACAGACAACCAAACCCTAGTCTCAGCCGGTGGAATTTTCGAGCTTGGCTTCTTCACCGAAACTATTTCGGGATATCACTACCTGGGAATTTGGTTCAAAGCTGATGCCACCAAGGTTGTCTGGGTTGGTAACCGCGATGTTGCTATATTGGATTCCTCCGGTGTGCTACAAATCCGGTCTGGGAATCTGCTTCTCTCTGACCGTCGACTAGTGCAAGTCATAGTCAACGCAGCAAATGTTGCCTCCTCACCCAACACAACTGCAACGCTTCTGGACACGGGAAATTTTGTGCTTAAAGAAGCAGATACAGGTACTGTTATATGGCAAAGTTTTGATGTACCAAGTGATACATATCTTCCTGGGATGGAACTAGGGTTGTTTGAACTAAACACAACCCAGCCAAGCTATAACATCCTTGTTTCTTGGGCAAGCCCCCAAAACCCAGCTCGCGGTCCCTTCACTTTAACCATGGACCGGATCAACTTCACAAAGCTTACTGTTTGGAGAGGAGATGGACGCCAGATGGATATCGCGTTCTGGGATGGACAACACCTAAGGTTTATTTTTGACAACACAACTGAAAATAATGACTACACTTTTAGGTACCAAACCATTGATGAAGAAGCTGCCTACTACACTTTTAGCAACAATAAGAAGTACGACCTCATATGGTTTGTCATGTCTTCAACTGGAAATTTGGACCAGTTTTTTTTGGCGAACGGGAATATTTGGTCTGTGAGTCATGGTTTGTGTGAGGACTCGAGCGGGGGTAATACTGGAAAATGTTTGGCGACTTTACCATCTATGTGTGACCATGGCGATGATTTTTCTGTGATGAATGGTTCGTTGCCATCTACGTCTAATAATGGATCCATTTATATGGGTACTAGTGATTGTGAAACTTTGTGCAAGAGCAATTGTTCTTGCACTGCATTTGTTGCGGTTCAGGATGGTCAACCAGTTTGTCAACTTTATTATCAGAGTAAGAAGGATCTTTTGAAGATTGTTGATGAGAAGGGCACTGGGATTATTTACATTCGTGGTGGTAGTACTTCTTCAAGTGATGGTAAAAAATTGAAACTCTGGCTGGCTATCGCAGTTCCTTTGGCTTTCCTCTTGATTCTTATGCCAATATCATTATGCTGCTATCTGCATTATGGAAATAGACTTCAAGGAGATGAAGCAAGGAAACAAGAAGAGATTATCAATTCGGATCAAGTGAGATTAGTCCAAATTGGTTCCACCAACGTGTCACCAATTCAATATAACGAAGATAGAATTGTGAATATGATGGAATTAGGCAGGCAAAAGGATCAAGAACTACCCTTGTTTAGTTTTTCTACCATACAAACTGCGACCAATGACTTTGCCAAGGCTAATAAACTGGGTGAAGGTGGATTTGGCCCTGTTTATAAGGGTTTGCTAGTGGATGGGAGGGAAATTGCAGTGAAAAGGTTGTCCGAAATTTCGAGGCAAGGTTTGGAGGAGTTCAAAAACGAAGTGTTAGTTATTTGTAAGCTTCAGCACAGGAATTTGGTTAGGCTTTTGGGATGCTGCATTGAAGGAGAAGAAAGTATATTAATTTATGAGTATATGCCCAACAAAAGCCTGGATTCTTTCATTTTTGATTCAACCAAACGGAAGCTTTTGGATTGGAGAAAGCGCATGAACATTATTGAAGGGATTGCTCAAGGCATCCTGTATCTTCACAAATACTCAAGATTAAGGATCATTCACCGCGATTTAAAGACAAGCAATATTCTCTTGGATAGTGACATGAACCCTAAAATATCGGATTTTGGCATGGCAAGAATTTTCGCAGACAGTGACACTAAAGGAAAAACAAGCCGGGTTGTTGGTACATTTGGTTATATGTCTCCTGAGTATGCCATGGGCGGCCTCTTTTCCGAAAAATCAGATGTGTTTAGCTTTGGAGTGATCTTATTAGAGATCATAAGTGGCAAGAAGAACATTGCCTTCTTTGAGGCTGATGATCCATTAAACTTACTAGGCAATGCCTGGAGCTTGTGGAAAGAAGGCAAGAGCAAGGAATTGATGGATTCGACATTGTCTGGTTCTTGTTCGAGTAATGAAGTTACCAGATGCATTCAGGTGGGTCTTTTGTGTGTGCAAGAAAGAGCTATGGATCGACCAAACATGTCGGATGTTGTTTCGATGCTAAGCAACGAAACATTTGCTCTGCCTCTTCCTAAAGAACCTGCATCTTGGAGTCAGCTAAGTTCCGCTGATGCAGATTCATCTTCAAGTAGGCAAAGACATCAATCTACAATAGATGTGACGACTTCAACAATACATGCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin PAN_2 Pkinase_Tyr

Protein Analysis

809

Amino Acids

89.81

Weight (kDa)

5.29

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 176 5.2e-24 D-mannose binding lectin
Pkinase PF00069 491 - 691 1.8e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 493 - 761 3.5e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000509)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29542 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29544 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29560
malus_domestica MD15G1121300.v1.1
prunus_persica Prupe.1G473900_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474100_v2.0.a1 Prupe.1G474300_v2.0.a1 Prupe.1G474500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298411 RchiOBHm_Chr6g0298421 RchiOBHm_Chr6g0298431 RchiOBHm_Chr6g0298441 RchiOBHm_Chr6g0298451 RchiOBHm_Chr6g0298461 RchiOBHm_Chr6g0298471 RchiOBHm_Chr6g0298481 RchiOBHm_Chr6g0298491 RchiOBHm_Chr6g0298501 RchiOBHm_Chr6g0298521
rosa_laevigata RLG00000011506 RLG00000011508 RLG00000011509 RLG00000011511 RLG00000011513 RLG00000011514 RLG00000011515
rosa_multiflora Rmu_co8314937.1_g000001 Rmu_co8319199.1_g000001 Rmu_sc0004500.1_g000002 Rmu_sc0006543.1_g000001 Rmu_ssc0000289.1_g000039 Rmu_ssc0000289.1_g000040 Rmu_ssc0000289.1_g000047 Rmu_ssc0000289.1_g000048 Rmu_ssc0000289.1_g000049 Rmu_ssc0000289.1_g000051
rosa_roxburghii Rroxscaffold_7G00169570 Rroxscaffold_7G00169580 Rroxscaffold_7G00169600 Rroxscaffold_7G00169610 Rroxscaffold_7G00169620 Rroxscaffold_7G00169630 Rroxscaffold_7G00169650 Rroxscaffold_7G00169660 Rroxscaffold_7G00169670
rosa_rugosa Rorug06G0280700 Rorug06G0280800 Rorug06G0280900
rosa_samantha Rh6AG391700 Rh6AG391800 Rh6AG392000 Rh6AG392100 Rh6AG392200 Rh6BG399300 Rh6BG399400 Rh6BG399500 Rh6BG399700 Rh6BG400000 Rh6CG405400 Rh6CG405500 Rh6CG405600 Rh6CG405700 Rh6CG405800 Rh6DG391800 Rh6DG391900 Rh6DG392000 Rh6DG392100 Rh6DG392300
rosa_wichuraiana Rw6G034230 Rw6G034240 Rw6G034250 Rw6G034260 Rw6G034270 Rw6G034280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1515
AarI CACCTGC 1 cut(s) 97
Acc36I ACCTGC 2 cut(s) 97, 2329
Acc65I GGTACC 1 cut(s) 705
AccB1I GGYRCC 1 cut(s) 705
AccBSI CCGCTC 1 cut(s) 867
AccI GTMKAC 1 cut(s) 307
AccII CGCG 4 cut(s) 237, 552, 635, 1842
AciI CCGC 7 cut(s) 235, 552, 867, 1043, 1840, 1998, 2346
AclWI GGATC 9 cut(s) 587, 966, 979, 1101, 1323, 1422, 1838, 2090, 2256
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 7 cut(s) 135, 189, 385, 796, 1566, 1633, 1914
AcuI CTGAAG 1 cut(s) 1607
AcyI GRCGYC 1 cut(s) 619
AfaI GTAC 7 cut(s) 415, 441, 707, 761, 988, 1153, 1963
AfiI CCNNNNNNNGG 2 cut(s) 551, 576
AflIII ACRYGT 2 cut(s) 1350, 2259
AhlI ACTAGT 2 cut(s) 310, 989
AjnI CCWGG 4 cut(s) 183, 460, 1710, 2120
AjuI GAANNNNNNNTTGG 2 cut(s) 1485, 1517
AleI CACNNNNGTG 1 cut(s) 1349
AloI GAACNNNNNNTCC 2 cut(s) 620, 652
Alw26I GTCTC 2 cut(s) 127, 1983
AlwI GGATC 9 cut(s) 587, 966, 979, 1101, 1323, 1422, 1838, 2090, 2256
AlwNI CAGNNNCTG 1 cut(s) 416
Ama87I CYCGRG 1 cut(s) 862
AoxI GGCC 3 cut(s) 3, 1504, 1999
ApeKI GCWGC 4 cut(s) 332, 728, 1245, 1656
ApoI RAATTY 7 cut(s) 135, 189, 385, 796, 1566, 1633, 1914
AseI ATTAAT 1 cut(s) 1682
Asp718I GGTACC 1 cut(s) 705
AspLEI GCGC 1 cut(s) 1767
AspS9I GGNCC 4 cut(s) 554, 574, 802, 1505
AsuC2I CCSGG 1 cut(s) 1952
AsuHPI GGTGA 5 cut(s) 148, 342, 1347, 1502, 1829
AvaI CYCGRG 1 cut(s) 862
AvaII GGWCC 3 cut(s) 554, 574, 802
AxyI CCTNAGG 1 cut(s) 656
BaeGI GKGCMC 1 cut(s) 1124
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 971
BanI GGYRCC 1 cut(s) 705
BarI GAAGNNNNNNTAC 2 cut(s) 1141, 1173
BbsI GAAGAC 1 cut(s) 777
BbvI GCAGC 4 cut(s) 344, 715, 1232, 1643
BcgI CGANNNNNNTGC 2 cut(s) 2279, 2313
BciT130I CCWGG 4 cut(s) 185, 462, 1712, 2122
BcnI CCSGG 1 cut(s) 1952
BcoDI GTCTC 2 cut(s) 127, 1983
BcuI ACTAGT 2 cut(s) 310, 989
BfaI CTAG 7 cut(s) 119, 311, 473, 990, 1526, 2111, 2421
BfmI CTRYAG 1 cut(s) 90
BfuAI ACCTGC 2 cut(s) 97, 2329
BisI GCNGC 5 cut(s) 333, 729, 1246, 1657, 1999
BlpI GCTNAGC 1 cut(s) 2282
BlsI GCNGC 5 cut(s) 334, 730, 1247, 1658, 2000
BmcAI AGTACT 1 cut(s) 1153
Bme1390I CCNGG 5 cut(s) 185, 462, 1712, 1952, 2122
Bme18I GGWCC 3 cut(s) 554, 574, 802
BmeT110I CYCGRG 1 cut(s) 862
BmgT120I GGNCC 4 cut(s) 554, 574, 802, 1505
BmiI GGNNCC 4 cut(s) 556, 707, 973, 1342
BmrFI CCNGG 5 cut(s) 185, 462, 1712, 1952, 2122
BmrI ACTGGG 2 cut(s) 1134, 1496
BmsI GCATC 7 cut(s) 196, 1643, 1806, 2195, 2268, 2333, 2341
BmuI ACTGGG 2 cut(s) 1134, 1496
BpiI GAAGAC 1 cut(s) 777
BpmI CTGGAG 1 cut(s) 2143
Bpu1102I GCTNAGC 1 cut(s) 2282
BpuEI CTTGAG 2 cut(s) 1776, 1804
BpuMI CCSGG 1 cut(s) 1952
BsaBI GATNNNNATC 2 cut(s) 453, 629
BsaHI GRCGYC 1 cut(s) 619
BsaJI CCNNGG 7 cut(s) 184, 213, 461, 570, 916, 1473, 1992
BsaWI WCCGGW 3 cut(s) 260, 276, 576
Bsc4I CCNNNNNNNGG 2 cut(s) 551, 576
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 6 cut(s) 796, 805, 883, 1061, 1129, 1491
Bse21I CCTNAGG 1 cut(s) 656
Bse3DI GCAATG 2 cut(s) 2073, 2122
Bse8I GATNNNNATC 2 cut(s) 453, 629
BseBI CCWGG 4 cut(s) 185, 462, 1712, 2122
BseDI CCNNGG 7 cut(s) 184, 213, 461, 570, 916, 1473, 1992
BseGI GGATG 8 cut(s) 471, 513, 649, 1057, 1537, 1658, 1797, 2272
BseJI GATNNNNATC 2 cut(s) 453, 629
BseLI CCNNNNNNNGG 2 cut(s) 551, 576
BseMI GCAATG 2 cut(s) 2073, 2122
BseMII CTCAG 2 cut(s) 138, 1974
BseNI ACTGG 6 cut(s) 796, 805, 883, 1061, 1129, 1491
BseRI GAGGAG 3 cut(s) 337, 624, 1601
BseSI GKGCMC 1 cut(s) 1124
BseXI GCAGC 4 cut(s) 344, 715, 1232, 1643
BseYI CCCAGC 2 cut(s) 498, 544
Bsh1236I CGCG 4 cut(s) 237, 552, 635, 1842
BshFI GGCC 3 cut(s) 5, 1506, 2001
BshNI GGYRCC 1 cut(s) 705
BsiHKCI CYCGRG 1 cut(s) 862
BsiSI CCGG 5 cut(s) 129, 261, 277, 577, 1951
BslFI GGGAC 1 cut(s) 540
BslI CCNNNNNNNGG 2 cut(s) 551, 576
BsmAI GTCTC 2 cut(s) 127, 1983
BsmFI GGGAC 1 cut(s) 540
BsmI GAATGC 1 cut(s) 2208
BsnI GGCC 3 cut(s) 5, 1506, 2001
BsoBI CYCGRG 1 cut(s) 862
Bsp1286I GDGCHC 1 cut(s) 1124
Bsp1720I GCTNAGC 1 cut(s) 2282
Bsp19I CCATGG 3 cut(s) 570, 916, 1992
BspACI CCGC 7 cut(s) 235, 552, 867, 1043, 1840, 1998, 2346
BspANI GGCC 3 cut(s) 5, 1506, 2001
BspCNI CTCAG 2 cut(s) 137, 1975
BspFNI CGCG 4 cut(s) 237, 552, 635, 1842
BspLI GGNNCC 4 cut(s) 556, 707, 973, 1342
BspMAI CTGCAG 1 cut(s) 94
BspMI ACCTGC 2 cut(s) 97, 2329
BspPI GGATC 9 cut(s) 587, 966, 979, 1101, 1323, 1422, 1838, 2090, 2256
BspT107I GGYRCC 1 cut(s) 705
BsrBI CCGCTC 1 cut(s) 867
BsrDI GCAATG 2 cut(s) 2073, 2122
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 6 cut(s) 796, 805, 883, 1061, 1129, 1491
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 7 cut(s) 184, 213, 461, 570, 916, 1473, 1992
BssNI GRCGYC 1 cut(s) 619
BssT1I CCWWGG 5 cut(s) 213, 570, 916, 1473, 1992
Bst2UI CCWGG 4 cut(s) 185, 462, 1712, 2122
Bst4CI ACNGT 4 cut(s) 305, 418, 601, 1928
Bst6I CTCTTC 4 cut(s) 49, 58, 1293, 2313
BstACI GRCGYC 1 cut(s) 619
BstAPI GCANNNNNTGC 2 cut(s) 341, 1040
BstC8I GCNNGC 4 cut(s) 532, 550, 1191, 1406
BstDEI CTNAG 5 cut(s) 124, 656, 1983, 2282, 2339
BstDSI CCRYGG 3 cut(s) 570, 916, 1992
BstEII GGTNACC 1 cut(s) 230
BstF5I GGATG 8 cut(s) 471, 513, 649, 1057, 1537, 1658, 1797, 2272
BstFNI CGCG 4 cut(s) 237, 552, 635, 1842
BstHHI GCGC 1 cut(s) 1767
BstMAI GTCTC 2 cut(s) 127, 1983
BstMWI GCNNNNNNNGC 2 cut(s) 341, 1040
BstNI CCWGG 4 cut(s) 185, 462, 1712, 2122
BstNSI RCATGY 3 cut(s) 100, 2263, 2420
BstPI GGTNACC 1 cut(s) 230
BstSCI CCNGG 5 cut(s) 183, 460, 1710, 1950, 2120
BstSFI CTRYAG 1 cut(s) 90
BstSLI GKGCMC 1 cut(s) 1124
BstUI CGCG 4 cut(s) 237, 552, 635, 1842
BstV1I GCAGC 4 cut(s) 344, 715, 1232, 1643
BstV2I GAAGAC 1 cut(s) 777
BstX2I RGATCY 2 cut(s) 971, 1093
BstXI CCANNNNNNTGG 1 cut(s) 982
BstYI RGATCY 2 cut(s) 971, 1093
Bsu36I CCTNAGG 1 cut(s) 656
BsuRI GGCC 3 cut(s) 5, 1506, 2001
BtgI CCRYGG 3 cut(s) 570, 916, 1992
BtgZI GCGATG 2 cut(s) 252, 936
BtsCI GGATG 8 cut(s) 471, 513, 649, 1057, 1537, 1658, 1797, 2272
BtsI GCAGTG 2 cut(s) 1029, 1554
BtsIMutI CAGTG 4 cut(s) 1029, 1122, 1554, 1933
BveI ACCTGC 2 cut(s) 97, 2329
Cac8I GCNNGC 4 cut(s) 532, 550, 1191, 1406
CaiI CAGNNNCTG 1 cut(s) 416
CfoI GCGC 1 cut(s) 1767
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 4 cut(s) 554, 574, 802, 1505
CseI GACGC 1 cut(s) 627
Csp6I GTAC 7 cut(s) 414, 440, 706, 760, 987, 1152, 1962
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviQI GTAC 7 cut(s) 414, 440, 706, 760, 987, 1152, 1962
DdeI CTNAG 5 cut(s) 124, 656, 1983, 2282, 2339
DraI TTTAAA 1 cut(s) 1848
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 49, 58, 1293, 2313
EarI CTCTTC 4 cut(s) 49, 58, 1293, 2313
Eco130I CCWWGG 5 cut(s) 213, 570, 916, 1473, 1992
Eco32I GATATC 2 cut(s) 176, 631
Eco47I GGWCC 3 cut(s) 554, 574, 802
Eco57I CTGAAG 1 cut(s) 1607
Eco81I CCTNAGG 1 cut(s) 656
Eco88I CYCGRG 1 cut(s) 862
Eco91I GGTNACC 1 cut(s) 230
EcoO65I GGTNACC 1 cut(s) 230
EcoRII CCWGG 4 cut(s) 183, 460, 1710, 2120
EcoRV GATATC 2 cut(s) 176, 631
EcoT14I CCWWGG 5 cut(s) 213, 570, 916, 1473, 1992
EcoT22I ATGCAT 1 cut(s) 2210
ErhI CCWWGG 5 cut(s) 213, 570, 916, 1473, 1992
FaqI GGGAC 1 cut(s) 540
FauI CCCGC 1 cut(s) 860
FauNDI CATATG 1 cut(s) 770
FblI GTMKAC 1 cut(s) 307
Fnu4HI GCNGC 5 cut(s) 333, 729, 1246, 1657, 1999
FokI GGATG 8 cut(s) 478, 500, 656, 1064, 1544, 1665, 1784, 2279
Fsp4HI GCNGC 5 cut(s) 333, 729, 1246, 1657, 1999
FspBI CTAG 7 cut(s) 119, 311, 473, 990, 1526, 2111, 2421
GlaI GCGC 1 cut(s) 1766
GluI GCNGC 5 cut(s) 333, 729, 1246, 1657, 1999
GsaI CCCAGC 2 cut(s) 502, 548
GsuI CTGGAG 1 cut(s) 2143
HaeIII GGCC 3 cut(s) 5, 1506, 2001
HapII CCGG 5 cut(s) 129, 261, 277, 577, 1951
HgaI GACGC 1 cut(s) 627
HhaI GCGC 1 cut(s) 1767
Hin1I GRCGYC 1 cut(s) 619
Hin6I GCGC 1 cut(s) 1765
HinP1I GCGC 1 cut(s) 1765
HincII GTYRAC 4 cut(s) 308, 328, 1058, 1070
HindII GTYRAC 4 cut(s) 308, 328, 1058, 1070
HindIII AAGCTT 3 cut(s) 593, 1619, 1745
HpaII CCGG 5 cut(s) 129, 261, 277, 577, 1951
HphI GGTGA 5 cut(s) 148, 342, 1347, 1502, 1829
Hpy166II GTNNAC 4 cut(s) 308, 328, 1058, 1070
Hpy188I TCNGA 8 cut(s) 301, 1083, 1315, 1564, 1897, 2011, 2020, 2266
Hpy188III TCNNGA 8 cut(s) 31, 171, 374, 1049, 1219, 1418, 1821, 1982
Hpy8I GTNNAC 4 cut(s) 308, 328, 1058, 1070
Hpy99I CGWCG 1 cut(s) 309
HpyAV CCTTC 9 cut(s) 58, 568, 1084, 1111, 1487, 1658, 1775, 2089, 2132
HpyCH4III ACNGT 4 cut(s) 305, 418, 601, 1928
HpyCH4IV ACGT 2 cut(s) 959, 1350
HpyF10VI GCNNNNNNNGC 2 cut(s) 341, 1040
HpyF3I CTNAG 5 cut(s) 124, 656, 1983, 2282, 2339
HpySE526I ACGT 2 cut(s) 959, 1350
Hsp92I GRCGYC 1 cut(s) 619
HspAI GCGC 1 cut(s) 1765
KpnI GGTACC 1 cut(s) 709
LmnI GCTCC 1 cut(s) 2124
Lsp1109I GCAGC 4 cut(s) 344, 715, 1232, 1643
LweI GCATC 7 cut(s) 196, 1643, 1806, 2195, 2268, 2333, 2341
MaeI CTAG 7 cut(s) 119, 311, 473, 990, 1526, 2111, 2421
MaeII ACGT 2 cut(s) 959, 1350
MaeIII GTNAC 7 cut(s) 230, 911, 1353, 1874, 1928, 2197, 2398
MbiI CCGCTC 1 cut(s) 867
MfeI CAATTG 1 cut(s) 1018
MflI RGATCY 2 cut(s) 971, 1093
MhlI GDGCHC 1 cut(s) 1124
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 850, 854, 2341
MmeI TCCRAC 1 cut(s) 2244
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 2210
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 396, 566, 1682, 1826, 1847, 2102
MslI CAYNNNNRTG 5 cut(s) 907, 921, 980, 1349, 2213
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 2348
MspI CCGG 5 cut(s) 129, 261, 277, 577, 1951
MspR9I CCNGG 5 cut(s) 185, 462, 1712, 1952, 2122
MunI CAATTG 1 cut(s) 1018
Mva1269I GAATGC 1 cut(s) 2208
MvaI CCWGG 4 cut(s) 185, 462, 1712, 2122
MvnI CGCG 4 cut(s) 237, 552, 635, 1842
MwoI GCNNNNNNNGC 2 cut(s) 341, 1040
NciI CCSGG 1 cut(s) 1952
NcoI CCATGG 3 cut(s) 570, 916, 1992
NdeI CATATG 1 cut(s) 770
NlaIV GGNNCC 4 cut(s) 556, 707, 973, 1342
NmuCI GTSAC 5 cut(s) 911, 1353, 1874, 1928, 2398
NsiI ATGCAT 1 cut(s) 2210
NspI RCATGY 3 cut(s) 100, 2263, 2420
OliI CACNNNNGTG 1 cut(s) 1349
PaeR7I CTCGAG 1 cut(s) 862
PaqCI CACCTGC 1 cut(s) 97
PciI ACATGT 1 cut(s) 2259
PctI GAATGC 1 cut(s) 2208
PfeI GAWTC 7 cut(s) 254, 286, 1222, 1715, 1730, 2162, 2357
PflFI GACNNNGTC 1 cut(s) 2170
PkrI GCNGC 5 cut(s) 334, 730, 1247, 1658, 2000
PleI GAGTC 3 cut(s) 849, 854, 2340
PpsI GAGTC 3 cut(s) 849, 854, 2340
PscI ACATGT 1 cut(s) 2259
PshBI ATTAAT 1 cut(s) 1682
PsiI TTATAA 1 cut(s) 1515
Psp6I CCWGG 4 cut(s) 183, 460, 1710, 2120
PspEI GGTNACC 1 cut(s) 230
PspFI CCCAGC 2 cut(s) 498, 544
PspGI CCWGG 4 cut(s) 183, 460, 1710, 2120
PspN4I GGNNCC 4 cut(s) 556, 707, 973, 1342
PspPI GGNCC 4 cut(s) 554, 574, 802, 1505
PspXI VCTCGAGB 1 cut(s) 862
PstI CTGCAG 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 2 cut(s) 971, 1093
PsyI GACNNNGTC 1 cut(s) 2170
RsaI GTAC 7 cut(s) 415, 441, 707, 761, 988, 1153, 1963
RsaNI GTAC 7 cut(s) 414, 440, 706, 760, 987, 1152, 1962
RseI CAYNNNNRTG 5 cut(s) 907, 921, 980, 1349, 2213
SalI GTCGAC 1 cut(s) 306
SaqAI TTAA 6 cut(s) 396, 566, 1682, 1826, 1847, 2102
SatI GCNGC 5 cut(s) 333, 729, 1246, 1657, 1999
Sau96I GGNCC 4 cut(s) 554, 574, 802, 1505
ScaI AGTACT 1 cut(s) 1153
SchI GAGTC 3 cut(s) 850, 854, 2341
ScrFI CCNGG 5 cut(s) 185, 462, 1712, 1952, 2122
SduI GDGCHC 1 cut(s) 1124
SfaNI GCATC 7 cut(s) 196, 1643, 1806, 2195, 2268, 2333, 2341
SfcI CTRYAG 1 cut(s) 90
Sfr274I CTCGAG 1 cut(s) 862
SinI GGWCC 3 cut(s) 554, 574, 802
SlaI CTCGAG 1 cut(s) 862
SmiMI CAYNNNNRTG 5 cut(s) 907, 921, 980, 1349, 2213
SmlI CTYRAG 3 cut(s) 862, 1791, 1819
SmoI CTYRAG 3 cut(s) 862, 1791, 1819
SpeI ACTAGT 2 cut(s) 310, 989
SsiI CCGC 7 cut(s) 235, 552, 867, 1043, 1840, 1998, 2346
SspI AATATT 2 cut(s) 829, 1861
SspMI CTAG 7 cut(s) 119, 311, 473, 990, 1526, 2111, 2421
StyD4I CCNGG 5 cut(s) 183, 460, 1710, 1950, 2120
StyI CCWWGG 5 cut(s) 213, 570, 916, 1473, 1992
TaaI ACNGT 4 cut(s) 305, 418, 601, 1928
TaiI ACGT 2 cut(s) 962, 1353
TaqI TCGA 8 cut(s) 141, 307, 863, 1571, 2165, 2186, 2251, 2276
TatI WGTACW 1 cut(s) 1151
TauI GCSGC 1 cut(s) 2001
TfiI GAWTC 7 cut(s) 254, 286, 1222, 1715, 1730, 2162, 2357
Tru1I TTAA 6 cut(s) 396, 566, 1682, 1826, 1847, 2102
Tru9I TTAA 6 cut(s) 396, 566, 1682, 1826, 1847, 2102
TscAI CASTG 4 cut(s) 1036, 1129, 1554, 1933
TseFI GTSAC 5 cut(s) 911, 1353, 1874, 1928, 2398
TseI GCWGC 4 cut(s) 332, 728, 1245, 1656
Tsp45I GTSAC 5 cut(s) 911, 1353, 1874, 1928, 2398
TspDTI ATGAA 9 cut(s) 45, 735, 953, 1296, 1711, 1784, 1895, 2208, 2349
TspGWI ACGGA 1 cut(s) 1756
TspRI CASTG 4 cut(s) 1036, 1129, 1554, 1933
Tth111I GACNNNGTC 1 cut(s) 2170
VpaK11BI GGWCC 3 cut(s) 554, 574, 802
VspI ATTAAT 1 cut(s) 1682
XapI RAATTY 7 cut(s) 135, 189, 385, 796, 1566, 1633, 1914
XceI RCATGY 3 cut(s) 100, 2263, 2420
XcmI CCANNNNNNNNNTGG 1 cut(s) 1708
XhoI CTCGAG 1 cut(s) 862
XmiI GTMKAC 1 cut(s) 307
XspI CTAG 7 cut(s) 119, 311, 473, 990, 1526, 2111, 2421
ZrmI AGTACT 1 cut(s) 1153
Zsp2I ATGCAT 1 cut(s) 2210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.