Rw6G034250

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000509
Physical Location & Seq
Reverse (-)
58841751 .. 58844999
3249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G034250.1

Sequence Viewer

Length: 2394 bp
ATGGCCATGTCTCTTCCTCTAATTTGCTTGATATCATCATTCTTTAGCTTCTTTTCGTTTTCATCTCTTGCAACAGATATTCTTACACCTTCAGACACATTGAGAGATGGTCAAATCCTGGTTTCAGCTGGTGGAGTTTTTGAGCTTGGCTTCTTCGCTGAAAGCTTTTCAGGCTATCACTATCTGGGAATTTGGTTGAGAGCCGATGCAAGCAAGGTTGTCTGGGTTGATCGCGATACTCCCATAATGGATTCATCAGGCATGCTTCAAATCCTGTCTGGTAATTTGGTTCTCACAGACCGTCGACAAGTGCAATTGATAGTCAACACAGGAAGTCTTGCCACGGCCTCAACTGCTAATACAAGTGCAACACTTCTTGATACTGGAAATTTTGTCCTCAAGGAAGCAGATACAGGTAATACTATATGGCAAAGTTTTGATATACCGACTGATACTTATCTTCCTGGGATGAAACTTGGGCTGTTTGGCCGAAACATATTTCACAGTATTGTTTCTTGGGCAAGCCCTCAAAACCCAGCTCGTGGCATCTTCACCTTAAGCATCAATAGCAACGACACCAGAAAGCTTTCAGTTCAGCGAGGAAATAATGGAGAGAAAATAGGTATCAGTTTCTGGGATGGAAATGGCATCCGGTTTATATTTGAAAATTCAACTGCCACTATGTACAATTTTAGCTTCCAGTCCAATGCAACCGAAGCATTCTACACTTTTACTACCAGTAAGAATTATGACCTTGTGTGGTTTGTGATGAATTACACTGGAAACCTGGACCAATATTTTATGGTGGATGGGAAGATCTCGTATGTGAGTCATGCTTTGTGTGAGGAGTCAGCCGGTGGTAGTACTCGGACGTGTTTTAGTACAATACCTTCTAACTGTGAAGATGGTGCCGAATTTTCTCAGATTAATGGTTTGCTGCCAGATACATCTAATACCACAGGTTCCCTAAATATGGTGATTACTGATTGTGAAACTTTCTGCAAGAACAATTGTTCTTGTACCGCATTCGCTTCAGTTCAGAATGGTCAACCAGGATGTCAACTTTACTATGGGAGTAAACAGGATTTATTGAAGAACATAGAGAAGGGGGCAGGGCTTATTTACATCCGGGGTGGTGCTCCAAGTGATGCTAAGAAGTGGAAACTCAGGTTGGCTGTGGCGATTCTTGTAGCTTCTCTCTTGGTTCTTATTCCAATCTTCTTCACCTGCTACCTGTGCTGGAGAAAGCGACTGAAAGAAGAACAAAGTCAACAAGAAGCCATTTTCAATTCTAATCAAGTGAGATTATTCCATGCGTCGTCATTTCATCACCATGAAGTAGGAGGTGTGAATAACATGGAACTAGGCATACACAAGGATAAAGAATTGCCCCTTTTTAGTTTTGCCAACATAAAGAAGGCAACAAATTACTTTGCAGATTCTAATAAACTTGGAGAAGGTGGATATGGGCCTGTCTATAAGGGTACGTTGCTAGTTGAAGGACATGAAATTGCAGTGAAAAGGCTGTCCAAGATTTCCAGGCAAGGGTTGAGGGAGTTCAAAAACGAAGTCTCACTGATTTGTAAGCTTCAACATAGAAACTTGGTTAGGCTTCTGGGATGCTGCATTGAAGCAGAAGAAAGCATACTGATTTACGAGTACATGCCCAACAAAAGTCTAGATTTCTTCATTTTTGACTCAACCAAACAGGTACTTTTGGATTGGAGAAGGCGTGTTAACATTATTGAAGGGATTGCACAAGGTCTTCTATATCTTCACAAATACTCGAGATTAAGGATCATTCACCGTGATTTAAAGGCCAGCAATATTCTGCTGGACAGTGACATGAACCCCAAGATTTCTGATTTTGGCATGGCAAGAATTTTCAGGGACAATGACATTAGAGGAAAAACAAACCGGGTTGTTGGTACATTTGGTTATATGTCTCCTGAATACGCAATGGGCGGTCTCTTTTCTGAAAAATCCGATGTCTTTAGCTTTGGGGTGATCTTATTAGAGATCATAAGCGGCAAGAAGAATATTGCATTCTTTGAGGCTGATCACTCTCTGAACTTACTCAGCAAAGCTTGGCATTTGTGGAAAGAAGGAAGCAGCATGGAGTTAATGGATTCAACACTGCGTGCTTCGTGTTCAAGCAGTGAAGTTATCAGATGCATACAGATGGGTCTTCTGTGCGTACAAGAAAAAGCTATTGATCGACCAACCATGTCGGATGTTGTTTCTATGCTAAGCAATCAGCCATTTGCTCTGCCTCTTCCCAAAGAACCTGCATTTTTGAGTCATTCAAGCTCCACTGATGCAGATTCATCTTCCCGTAGGCAAAGATATTACTCTCAAAATGATATGACAATTTCAGAAGAATGTGGCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

797

Amino Acids

88.97

Weight (kDa)

6.59

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 173 1.7e-23 D-mannose binding lectin
PAN_2 PF08276 300 - 356 6.2e-07 PAN-like domain
Pkinase PF00069 480 - 747 2.2e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 482 - 750 7.3e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 756 - 792 1.6e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000509)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g29542 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29543 FvH4_2g29544 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29545 FvH4_2g29560
malus_domestica MD15G1121300.v1.1
prunus_persica Prupe.1G473900_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474000_v2.0.a1 Prupe.1G474100_v2.0.a1 Prupe.1G474300_v2.0.a1 Prupe.1G474500_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0298411 RchiOBHm_Chr6g0298421 RchiOBHm_Chr6g0298431 RchiOBHm_Chr6g0298441 RchiOBHm_Chr6g0298451 RchiOBHm_Chr6g0298461 RchiOBHm_Chr6g0298471 RchiOBHm_Chr6g0298481 RchiOBHm_Chr6g0298491 RchiOBHm_Chr6g0298501 RchiOBHm_Chr6g0298521
rosa_laevigata RLG00000011506 RLG00000011508 RLG00000011509 RLG00000011511 RLG00000011513 RLG00000011514 RLG00000011515
rosa_multiflora Rmu_co8314937.1_g000001 Rmu_co8319199.1_g000001 Rmu_sc0004500.1_g000002 Rmu_sc0006543.1_g000001 Rmu_ssc0000289.1_g000039 Rmu_ssc0000289.1_g000040 Rmu_ssc0000289.1_g000047 Rmu_ssc0000289.1_g000048 Rmu_ssc0000289.1_g000049 Rmu_ssc0000289.1_g000051
rosa_roxburghii Rroxscaffold_7G00169570 Rroxscaffold_7G00169580 Rroxscaffold_7G00169600 Rroxscaffold_7G00169610 Rroxscaffold_7G00169620 Rroxscaffold_7G00169630 Rroxscaffold_7G00169650 Rroxscaffold_7G00169660 Rroxscaffold_7G00169670
rosa_rugosa Rorug06G0280700 Rorug06G0280800 Rorug06G0280900
rosa_samantha Rh6AG391700 Rh6AG391800 Rh6AG392000 Rh6AG392100 Rh6AG392200 Rh6BG399300 Rh6BG399400 Rh6BG399500 Rh6BG399700 Rh6BG400000 Rh6CG405400 Rh6CG405500 Rh6CG405600 Rh6CG405700 Rh6CG405800 Rh6DG391800 Rh6DG391900 Rh6DG392000 Rh6DG392100 Rh6DG392300
rosa_wichuraiana Rw6G034230 Rw6G034240 Rw6G034250 Rw6G034260 Rw6G034270 Rw6G034280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1235
Acc36I ACCTGC 3 cut(s) 1235, 2296, 2379
AccB1I GGYRCC 1 cut(s) 908
AccB7I CCANNNNNTGG 1 cut(s) 542
AccI GTMKAC 1 cut(s) 304
AccII CGCG 1 cut(s) 234
AciI CCGC 3 cut(s) 1023, 1965, 2028
AclWI GGATC 1 cut(s) 1805
AcoI YGGCCR 2 cut(s) 3, 487
AcsI RAATTY 5 cut(s) 189, 388, 667, 914, 1881
AcuI CTGAAG 2 cut(s) 75, 1017
AdeI CACNNNGTG 1 cut(s) 2141
AfaI GTAC 9 cut(s) 686, 865, 883, 1021, 1486, 1661, 1713, 1930, 2199
AfiI CCNNNNNNNGG 3 cut(s) 542, 973, 1545
AflII CTTAAG 1 cut(s) 556
AflIII ACRYGT 1 cut(s) 872
AjiI CACGTC 1 cut(s) 873
AjnI CCWGG 5 cut(s) 117, 463, 786, 1051, 1538
AjuI GAANNNNNNNTTGG 2 cut(s) 1154, 1186
Alw21I GWGCWC 1 cut(s) 1141
Alw26I GTCTC 4 cut(s) 15, 1576, 1950, 1973
AlwI GGATC 1 cut(s) 1805
AlwNI CAGNNNCTG 1 cut(s) 633
Ama87I CYCGRG 1 cut(s) 1786
AoxI GGCC 5 cut(s) 3, 345, 487, 1469, 1818
ApeKI GCWGC 3 cut(s) 937, 1623, 2112
ApoI RAATTY 5 cut(s) 189, 388, 667, 914, 1881
ArsI GACNNNNNNTTYG 2 cut(s) 1554, 1586
AseI ATTAAT 1 cut(s) 927
Asp700I GAANNNNTTC 1 cut(s) 586
AspS9I GGNCC 2 cut(s) 790, 1469
AsuC2I CCSGG 2 cut(s) 1130, 1919
AsuHPI GGTGA 6 cut(s) 544, 988, 1216, 1322, 1796, 2017
AvaI CYCGRG 1 cut(s) 1786
AvaII GGWCC 1 cut(s) 790
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 908
BarI GAAGNNNNNNTAC 4 cut(s) 874, 906, 1629, 1661
BauI CACGAG 1 cut(s) 540
BbsI GAAGAC 2 cut(s) 1757, 2180
Bbv12I GWGCWC 1 cut(s) 1141
BbvI GCAGC 3 cut(s) 924, 1610, 2124
BccI CCATC 5 cut(s) 101, 632, 803, 899, 2176
BceAI ACGGC 1 cut(s) 360
BciT130I CCWGG 5 cut(s) 119, 465, 788, 1053, 1540
BclI TGATCA 1 cut(s) 2059
BcnI CCSGG 2 cut(s) 1130, 1919
BcoDI GTCTC 4 cut(s) 15, 1576, 1950, 1973
BfaI CTAG 3 cut(s) 1364, 1493, 1679
BfrI CTTAAG 1 cut(s) 556
BfuAI ACCTGC 3 cut(s) 1235, 2296, 2379
BglII AGATCT 1 cut(s) 816
BisI GCNGC 4 cut(s) 938, 1624, 2029, 2113
BlpI GCTNAGC 1 cut(s) 2249
BlsI GCNGC 4 cut(s) 939, 1625, 2030, 2114
BmcAI AGTACT 1 cut(s) 865
Bme1390I CCNGG 7 cut(s) 119, 465, 788, 1053, 1130, 1540, 1919
Bme18I GGWCC 1 cut(s) 790
BmeT110I CYCGRG 1 cut(s) 1786
BmgBI CACGTC 1 cut(s) 873
BmgT120I GGNCC 2 cut(s) 790, 1469
BmiI GGNNCC 2 cut(s) 910, 964
BmrFI CCNGG 7 cut(s) 119, 465, 788, 1053, 1130, 1540, 1919
BmsI GCATC 8 cut(s) 196, 555, 570, 657, 1138, 1610, 2162, 2308
BpiI GAAGAC 2 cut(s) 1757, 2180
BpmI CTGGAG 1 cut(s) 1261
Bpu1102I GCTNAGC 1 cut(s) 2249
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 2 cut(s) 1130, 1919
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 1973
BsaJI CCNNGG 3 cut(s) 342, 464, 1129
BsaWI WCCGGW 1 cut(s) 651
BsaXI ACNNNNNCTCC 2 cut(s) 1717, 1747
Bsc4I CCNNNNNNNGG 3 cut(s) 542, 973, 1545
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 4 cut(s) 388, 700, 738, 784
Bse3DI GCAATG 1 cut(s) 1965
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 5 cut(s) 119, 465, 788, 1053, 1540
BseDI CCNNGG 3 cut(s) 342, 464, 1129
BseGI GGATG 8 cut(s) 474, 643, 648, 814, 1061, 1125, 1625, 2239
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 3 cut(s) 542, 973, 1545
BseMI GCAATG 1 cut(s) 1965
BseMII CTCAG 3 cut(s) 935, 1180, 2092
BseNI ACTGG 4 cut(s) 388, 700, 738, 784
BseRI GAGGAG 1 cut(s) 860
BseXI GCAGC 3 cut(s) 924, 1610, 2124
BseYI CCCAGC 1 cut(s) 535
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 5 cut(s) 5, 347, 489, 1471, 1820
BshNI GGYRCC 1 cut(s) 908
BsiHKAI GWGCWC 1 cut(s) 1141
BsiHKCI CYCGRG 1 cut(s) 1786
BsiSI CCGG 4 cut(s) 652, 855, 1129, 1918
BslFI GGGAC 1 cut(s) 1904
BslI CCNNNNNNNGG 3 cut(s) 542, 973, 1545
BsmAI GTCTC 4 cut(s) 15, 1576, 1950, 1973
BsmFI GGGAC 1 cut(s) 1904
BsmI GAATGC 3 cut(s) 719, 1025, 2045
BsnI GGCC 5 cut(s) 5, 347, 489, 1471, 1820
Bso31I GGTCTC 1 cut(s) 1973
BsoBI CYCGRG 1 cut(s) 1786
Bsp1286I GDGCHC 1 cut(s) 1141
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 7 cut(s) 229, 816, 1797, 2007, 2019, 2059, 2215
Bsp1720I GCTNAGC 1 cut(s) 2249
Bsp68I TCGCGA 1 cut(s) 234
BspACI CCGC 3 cut(s) 1023, 1965, 2028
BspANI GGCC 5 cut(s) 5, 347, 489, 1471, 1820
BspCNI CTCAG 3 cut(s) 934, 1179, 2091
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 910, 964
BspMI ACCTGC 3 cut(s) 1235, 2296, 2379
BspPI GGATC 1 cut(s) 1805
BspT107I GGYRCC 1 cut(s) 908
BspTI CTTAAG 1 cut(s) 556
BspTNI GGTCTC 1 cut(s) 1973
BsrDI GCAATG 1 cut(s) 1965
BsrFI RCCGGY 1 cut(s) 854
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 4 cut(s) 388, 700, 738, 784
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 3 cut(s) 342, 464, 1129
BssMI GATC 7 cut(s) 229, 816, 1797, 2007, 2019, 2059, 2215
BssSI CACGAG 1 cut(s) 540
Bst2BI CACGAG 1 cut(s) 540
Bst2UI CCWGG 5 cut(s) 119, 465, 788, 1053, 1540
Bst4CI ACNGT 5 cut(s) 302, 506, 899, 1808, 1841
Bst6I CTCTTC 2 cut(s) 18, 2280
BstAFI CTTAAG 1 cut(s) 556
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 5 cut(s) 211, 263, 523, 1822, 2142
BstDEI CTNAG 5 cut(s) 921, 1152, 1166, 2078, 2249
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 8 cut(s) 474, 643, 648, 814, 1061, 1125, 1625, 2239
BstFNI CGCG 1 cut(s) 234
BstKTI GATC 7 cut(s) 232, 819, 1800, 2010, 2022, 2062, 2218
BstMAI GTCTC 4 cut(s) 15, 1576, 1950, 1973
BstMBI GATC 7 cut(s) 229, 816, 1797, 2007, 2019, 2059, 2215
BstMWI GCNNNNNNNGC 5 cut(s) 171, 353, 567, 716, 1236
BstNI CCWGG 5 cut(s) 119, 465, 788, 1053, 1540
BstNSI RCATGY 2 cut(s) 265, 1666
BstSCI CCNGG 7 cut(s) 117, 463, 786, 1051, 1128, 1538, 1917
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 3 cut(s) 924, 1610, 2124
BstV2I GAAGAC 2 cut(s) 1757, 2180
BstX2I RGATCY 1 cut(s) 816
BstYI RGATCY 1 cut(s) 816
BsuRI GGCC 5 cut(s) 5, 347, 489, 1471, 1820
BtgI CCRYGG 1 cut(s) 342
BtrI CACGTC 1 cut(s) 873
BtsCI GGATG 8 cut(s) 474, 643, 648, 814, 1061, 1125, 1625, 2239
BtsI GCAGTG 3 cut(s) 1521, 2135, 2164
BtsIMutI CAGTG 7 cut(s) 777, 1521, 1574, 1846, 2135, 2164, 2313
BtuMI TCGCGA 1 cut(s) 234
BveI ACCTGC 3 cut(s) 1235, 2296, 2379
Cac8I GCNNGC 5 cut(s) 211, 263, 523, 1822, 2142
CaiI CAGNNNCTG 1 cut(s) 633
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 2 cut(s) 790, 1469
CseI GACGC 1 cut(s) 1305
Csp6I GTAC 9 cut(s) 685, 864, 882, 1020, 1485, 1660, 1712, 1929, 2198
CviQI GTAC 9 cut(s) 685, 864, 882, 1020, 1485, 1660, 1712, 1929, 2198
DdeI CTNAG 5 cut(s) 921, 1152, 1166, 2078, 2249
DpnI GATC 7 cut(s) 231, 818, 1799, 2009, 2021, 2061, 2217
DpnII GATC 7 cut(s) 229, 816, 1797, 2007, 2019, 2059, 2215
DraI TTTAAA 1 cut(s) 1815
DraIII CACNNNGTG 1 cut(s) 2141
EaeI YGGCCR 2 cut(s) 3, 487
Eam1104I CTCTTC 2 cut(s) 18, 2280
EarI CTCTTC 2 cut(s) 18, 2280
Eco31I GGTCTC 1 cut(s) 1973
Eco32I GATATC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 790
Eco57I CTGAAG 2 cut(s) 75, 1017
Eco88I CYCGRG 1 cut(s) 1786
EcoRII CCWGG 5 cut(s) 117, 463, 786, 1051, 1538
EcoRV GATATC 1 cut(s) 33
EcoT22I ATGCAT 1 cut(s) 2177
FaqI GGGAC 1 cut(s) 1904
FbaI TGATCA 1 cut(s) 2059
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 4 cut(s) 938, 1624, 2029, 2113
FokI GGATG 8 cut(s) 481, 635, 650, 821, 1068, 1112, 1632, 2246
Fsp4HI GCNGC 4 cut(s) 938, 1624, 2029, 2113
FspBI CTAG 3 cut(s) 1364, 1493, 1679
GluI GCNGC 4 cut(s) 938, 1624, 2029, 2113
GsaI CCCAGC 1 cut(s) 539
GsuI CTGGAG 1 cut(s) 1261
HaeIII GGCC 5 cut(s) 5, 347, 489, 1471, 1820
HapII CCGG 4 cut(s) 652, 855, 1129, 1918
HgaI GACGC 1 cut(s) 1305
HincII GTYRAC 6 cut(s) 305, 325, 1049, 1061, 1271, 1738
HindII GTYRAC 6 cut(s) 305, 325, 1049, 1061, 1271, 1738
HindIII AAGCTT 4 cut(s) 163, 584, 1586, 2085
HinfI GANTC 9 cut(s) 251, 829, 848, 1183, 1439, 1697, 2129, 2299, 2324
HpaI GTTAAC 1 cut(s) 1738
HpaII CCGG 4 cut(s) 652, 855, 1129, 1918
HphI GGTGA 6 cut(s) 544, 988, 1216, 1322, 1796, 2017
Hpy166II GTNNAC 7 cut(s) 305, 325, 1049, 1061, 1079, 1271, 1738
Hpy188III TCNNGA 5 cut(s) 233, 377, 1679, 1788, 1949
Hpy8I GTNNAC 7 cut(s) 305, 325, 1049, 1061, 1079, 1271, 1738
Hpy99I CGWCG 2 cut(s) 306, 1321
HpyAV CCTTC 9 cut(s) 99, 900, 1099, 1411, 1451, 1493, 1722, 1742, 2099
HpyCH4III ACNGT 5 cut(s) 302, 506, 899, 1808, 1841
HpyCH4IV ACGT 2 cut(s) 872, 1487
HpyF10VI GCNNNNNNNGC 5 cut(s) 171, 353, 567, 716, 1236
HpyF3I CTNAG 5 cut(s) 921, 1152, 1166, 2078, 2249
HpySE526I ACGT 2 cut(s) 872, 1487
Ksp22I TGATCA 1 cut(s) 2059
KspAI GTTAAC 1 cut(s) 1738
Kzo9I GATC 7 cut(s) 229, 816, 1797, 2007, 2019, 2059, 2215
LmnI GCTCC 2 cut(s) 1144, 2315
Lsp1109I GCAGC 3 cut(s) 924, 1610, 2124
LweI GCATC 8 cut(s) 196, 555, 570, 657, 1138, 1610, 2162, 2308
MaeI CTAG 3 cut(s) 1364, 1493, 1679
MaeII ACGT 2 cut(s) 872, 1487
MaeIII GTNAC 1 cut(s) 1841
MalI GATC 7 cut(s) 231, 818, 1799, 2009, 2021, 2061, 2217
MboI GATC 7 cut(s) 229, 816, 1797, 2007, 2019, 2059, 2215
MfeI CAATTG 2 cut(s) 314, 1009
MflI RGATCY 1 cut(s) 816
MhlI GDGCHC 1 cut(s) 1141
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 838, 857, 1691, 2308
MmeI TCCRAC 1 cut(s) 2211
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 2177
MroXI GAANNNNTTC 1 cut(s) 586
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 557, 927, 1737, 1793, 1814, 2123
MslI CAYNNNNRTG 2 cut(s) 1332, 2180
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 128
MspCI CTTAAG 1 cut(s) 556
MspI CCGG 4 cut(s) 652, 855, 1129, 1918
MspR9I CCNGG 7 cut(s) 119, 465, 788, 1053, 1130, 1540, 1919
MunI CAATTG 2 cut(s) 314, 1009
Mva1269I GAATGC 3 cut(s) 719, 1025, 2045
MvaI CCWGG 5 cut(s) 119, 465, 788, 1053, 1540
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 5 cut(s) 171, 353, 567, 716, 1236
NciI CCSGG 2 cut(s) 1130, 1919
NdeII GATC 7 cut(s) 229, 816, 1797, 2007, 2019, 2059, 2215
NlaIV GGNNCC 2 cut(s) 910, 964
NmuCI GTSAC 1 cut(s) 1841
NruI TCGCGA 1 cut(s) 234
NsiI ATGCAT 1 cut(s) 2177
NspI RCATGY 2 cut(s) 265, 1666
PaeI GCATGC 1 cut(s) 265
PaeR7I CTCGAG 1 cut(s) 1786
PaqCI CACCTGC 1 cut(s) 1235
PctI GAATGC 3 cut(s) 719, 1025, 2045
PdmI GAANNNNTTC 1 cut(s) 586
PfeI GAWTC 5 cut(s) 251, 1183, 1439, 2129, 2324
PflMI CCANNNNNTGG 1 cut(s) 542
PkrI GCNGC 4 cut(s) 939, 1625, 2030, 2114
PleI GAGTC 4 cut(s) 837, 856, 1691, 2307
PpsI GAGTC 4 cut(s) 837, 856, 1691, 2307
PshBI ATTAAT 1 cut(s) 927
Psp6I CCWGG 5 cut(s) 117, 463, 786, 1051, 1538
PspFI CCCAGC 1 cut(s) 535
PspGI CCWGG 5 cut(s) 117, 463, 786, 1051, 1538
PspN4I GGNNCC 2 cut(s) 910, 964
PspPI GGNCC 2 cut(s) 790, 1469
PsrI GAACNNNNNNTAC 2 cut(s) 1353, 1385
PstNI CAGNNNCTG 1 cut(s) 633
PsuI RGATCY 1 cut(s) 816
PvuII CAGCTG 1 cut(s) 128
RruI TCGCGA 1 cut(s) 234
RsaI GTAC 9 cut(s) 686, 865, 883, 1021, 1486, 1661, 1713, 1930, 2199
RsaNI GTAC 9 cut(s) 685, 864, 882, 1020, 1485, 1660, 1712, 1929, 2198
RseI CAYNNNNRTG 2 cut(s) 1332, 2180
SalI GTCGAC 1 cut(s) 303
SaqAI TTAA 6 cut(s) 557, 927, 1737, 1793, 1814, 2123
SatI GCNGC 4 cut(s) 938, 1624, 2029, 2113
Sau3AI GATC 7 cut(s) 229, 816, 1797, 2007, 2019, 2059, 2215
Sau96I GGNCC 2 cut(s) 790, 1469
ScaI AGTACT 1 cut(s) 865
SchI GAGTC 4 cut(s) 838, 857, 1691, 2308
ScrFI CCNGG 7 cut(s) 119, 465, 788, 1053, 1130, 1540, 1919
SduI GDGCHC 1 cut(s) 1141
SfaNI GCATC 8 cut(s) 196, 555, 570, 657, 1138, 1610, 2162, 2308
Sfr274I CTCGAG 1 cut(s) 1786
SinI GGWCC 1 cut(s) 790
SlaI CTCGAG 1 cut(s) 1786
SmiMI CAYNNNNRTG 2 cut(s) 1332, 2180
SmlI CTYRAG 3 cut(s) 398, 556, 1786
SmoI CTYRAG 3 cut(s) 398, 556, 1786
SphI GCATGC 1 cut(s) 265
SsiI CCGC 3 cut(s) 1023, 1965, 2028
SspI AATATT 3 cut(s) 797, 1828, 2041
SspMI CTAG 3 cut(s) 1364, 1493, 1679
StyD4I CCNGG 7 cut(s) 117, 463, 786, 1051, 1128, 1538, 1917
TaaI ACNGT 5 cut(s) 302, 506, 899, 1808, 1841
TaiI ACGT 2 cut(s) 875, 1490
TaqI TCGA 3 cut(s) 304, 1787, 2218
TatI WGTACW 4 cut(s) 684, 863, 881, 1659
TauI GCSGC 1 cut(s) 2031
TfiI GAWTC 5 cut(s) 251, 1183, 1439, 2129, 2324
Tru1I TTAA 6 cut(s) 557, 927, 1737, 1793, 1814, 2123
Tru9I TTAA 6 cut(s) 557, 927, 1737, 1793, 1814, 2123
TscAI CASTG 7 cut(s) 784, 1521, 1581, 1846, 2142, 2164, 2320
TseFI GTSAC 1 cut(s) 1841
TseI GCWGC 3 cut(s) 937, 1623, 2112
Tsp45I GTSAC 1 cut(s) 1841
TspRI CASTG 7 cut(s) 784, 1521, 1581, 1846, 2142, 2164, 2320
Van91I CCANNNNNTGG 1 cut(s) 542
Vha464I CTTAAG 1 cut(s) 556
VpaK11BI GGWCC 1 cut(s) 790
VspI ATTAAT 1 cut(s) 927
XapI RAATTY 5 cut(s) 189, 388, 667, 914, 1881
XbaI TCTAGA 1 cut(s) 1678
XceI RCATGY 2 cut(s) 265, 1666
XhoI CTCGAG 1 cut(s) 1786
XmiI GTMKAC 1 cut(s) 304
XmnI GAANNNNTTC 1 cut(s) 586
XspI CTAG 3 cut(s) 1364, 1493, 1679
ZrmI AGTACT 1 cut(s) 865
Zsp2I ATGCAT 1 cut(s) 2177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.