FvH4_2g35800

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000672
Physical Location & Seq
Forward (+)
26366014 .. 26370050
4037 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35800.t1

Sequence Viewer

Length: 1233 bp
ATGATCGGAAAATTGAACATCATCGAGATCAAGAAATTGAACCAGCTTAAGAAAGGGAAGCTGGAGAAATTGATCGGGATCAAGATCGATCAAAACAAGCTCACTATCCGAGGGATGAAGACGGACGATCTAGATCTCGGCCTACGATCTGAACCGCTTCGCCCTAACGATCTGGAGAAGGCAAATATGAGAAACGAGACTATTGCTAATGGAGGTGTTTTGTCAAATATTCATCGGAAATCTGTTGTGGAAGAAGACTTTGGAGGAAACAAGAAATTCCGGAGCACCGTTGACGTAATTCCGATCAGATTCCGCTGGAGTACGGGATATTCAAACTACAATCCAGAAACCGATCAGTACGATCGTGACCCACCAATTCCGATTCTGAAACGTATTAGAACTCTCAAGTTGCGGTTCTCGAGGGAGAAGGCGAGTGGAGATGCAAAGACAAGGATGAAGAGATTTGCAAGGTACAAGAGGATCATAGCTGGGTCTCCGAGAGAAGCGAGCGAGTTACGGTACAAGTTGGGCAGGTATCTCTGCACAGCTATGCGTCTTCCCGAAGATAAGCACTCGTCTATTATTGATCAAATAGTAAGAAAACTTCAAGATAAAGAAGCCGACCCCGATAAAGTTATCGAGGTGGCGATCGTGGAGGTAATATTTAGAGATACGGAGGAGTCGTTGCTGTTGGACTTTGAGGACAACGACGATTACTATAGGGCAAAGCTGATTCCGGCAACTGAATCATTCATCCAGGCTTTGGAGGAAGCGAGTCCACCTGATGATGATGATGAATTAGAGTGTGCTATTTGTATGGAAATCAGATGGTTCCGTAGTCTTGTTAGGGGTGCTAGATTGTCTATCATAAGTGCTATGAATTTGGCTAGGATTCCAGACCAGCTTGGCTTGGGCCTTGAGGAGACGTCTGGAATGCTCCGGAGCGTCCGTGCTCCGCTCCTGCAACTGAAGAGACAAACAATGGAAGATCTGTGGAAACGACCCAATAGCTTTGCCGAAGAAGCAACCAAGAAATCAGACCATCACCGTTTCACCACCTCCCGCGAGTTCGCCGCCGAGCCCTCCAAGGAAGCCAACGAGATCAAAACGGCCGCTCTTAAGCAAGCCGATCAGATCAAGGAGAGGGAGATAGATCGATCTCATTCATTGATATTCCCATTAGACTATTCAGTGACCAATTTACCCACATCCAACTCGTCTACCCTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

411

Amino Acids

47.05

Weight (kDa)

8.84

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 929
Acc36I ACCTGC 1 cut(s) 522
AccB7I CCANNNNNTGG 1 cut(s) 763
AccBSI CCGCTC 2 cut(s) 958, 1115
AccI GTMKAC 1 cut(s) 1220
AccII CGCG 1 cut(s) 1065
AccIII TCCGGA 2 cut(s) 279, 939
AciI CCGC 7 cut(s) 155, 313, 412, 956, 1063, 1074, 1113
AclWI GGATC 2 cut(s) 86, 488
AcoI YGGCCR 1 cut(s) 1110
AcsI RAATTY 2 cut(s) 275, 880
AcuI CTGAAG 1 cut(s) 989
AcyI GRCGYC 1 cut(s) 926
AfaI GTAC 4 cut(s) 322, 359, 473, 521
AfiI CCNNNNNNNGG 1 cut(s) 763
AflII CTTAAG 2 cut(s) 47, 1118
AgsI TTSAA 4 cut(s) 16, 40, 333, 608
AjnI CCWGG 1 cut(s) 756
AjuI GAANNNNNNNTTGG 2 cut(s) 243, 275
AluBI AGCT 8 cut(s) 46, 61, 100, 488, 548, 730, 904, 1011
AluI AGCT 8 cut(s) 46, 61, 100, 488, 548, 730, 904, 1011
Alw21I GWGCWC 2 cut(s) 287, 955
Alw26I GTCTC 4 cut(s) 191, 498, 917, 967
AlwI GGATC 2 cut(s) 86, 488
Ama87I CYCGRG 1 cut(s) 418
Aor13HI TCCGGA 2 cut(s) 279, 939
AoxI GGCC 3 cut(s) 139, 913, 1110
ApoI RAATTY 2 cut(s) 275, 880
Asp700I GAANNNNTTC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 913
AsuHPI GGTGA 2 cut(s) 1037, 1045
AvaI CYCGRG 1 cut(s) 418
BanII GRGCYC 1 cut(s) 1083
BarI GAAGNNNNNNTAC 2 cut(s) 588, 620
BbsI GAAGAC 3 cut(s) 125, 261, 548
Bbv12I GWGCWC 2 cut(s) 287, 955
BccI CCATC 2 cut(s) 822, 1050
BceAI ACGGC 1 cut(s) 1125
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 1 cut(s) 758
BclI TGATCA 1 cut(s) 586
BcoDI GTCTC 4 cut(s) 191, 498, 917, 967
BfaI CTAG 3 cut(s) 131, 855, 888
BfmI CTRYAG 1 cut(s) 718
BfrI CTTAAG 2 cut(s) 47, 1118
BfuAI ACCTGC 1 cut(s) 522
BglII AGATCT 2 cut(s) 133, 988
BisI GCNGC 2 cut(s) 1074, 1113
BlsI GCNGC 2 cut(s) 1075, 1114
Bme1390I CCNGG 1 cut(s) 758
BmeT110I CYCGRG 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 913
BmiI GGNNCC 1 cut(s) 833
BmrFI CCNGG 1 cut(s) 758
BmsI GCATC 1 cut(s) 430
BpiI GAAGAC 3 cut(s) 125, 261, 548
BpmI CTGGAG 3 cut(s) 83, 194, 337
BpuEI CTTGAG 2 cut(s) 389, 938
Bsa29I ATCGAT 2 cut(s) 87, 1156
BsaBI GATNNNNATC 3 cut(s) 77, 83, 132
BsaHI GRCGYC 1 cut(s) 926
BsaI GGTCTC 1 cut(s) 498
BsaJI CCNNGG 2 cut(s) 109, 1086
BsaWI WCCGGW 2 cut(s) 279, 939
BsaXI ACNNNNNCTCC 4 cut(s) 668, 698, 934, 964
Bsc4I CCNNNNNNNGG 1 cut(s) 763
Bse8I GATNNNNATC 3 cut(s) 77, 83, 132
BseAI TCCGGA 2 cut(s) 279, 939
BseBI CCWGG 1 cut(s) 758
BseCI ATCGAT 2 cut(s) 87, 1156
BseDI CCNNGG 2 cut(s) 109, 1086
BseGI GGATG 4 cut(s) 120, 459, 753, 1208
BseJI GATNNNNATC 3 cut(s) 77, 83, 132
BseLI CCNNNNNNNGG 1 cut(s) 763
BseRI GAGGAG 2 cut(s) 692, 935
BseX3I CGGCCG 1 cut(s) 1110
BseYI CCCAGC 1 cut(s) 488
BsgI GTGCAG 1 cut(s) 526
Bsh1236I CGCG 1 cut(s) 1065
Bsh1285I CGRYCG 3 cut(s) 364, 651, 1113
BshFI GGCC 3 cut(s) 141, 915, 1112
BshVI ATCGAT 2 cut(s) 87, 1156
BsiEI CGRYCG 3 cut(s) 364, 651, 1113
BsiHKAI GWGCWC 2 cut(s) 287, 955
BsiHKCI CYCGRG 1 cut(s) 418
BsiSI CCGG 3 cut(s) 280, 737, 940
BslI CCNNNNNNNGG 1 cut(s) 763
BsmAI GTCTC 4 cut(s) 191, 498, 917, 967
BsmBI CGTCTC 1 cut(s) 917
BsmI GAATGC 1 cut(s) 939
BsnI GGCC 3 cut(s) 141, 915, 1112
Bso31I GGTCTC 1 cut(s) 498
BsoBI CYCGRG 1 cut(s) 418
Bsp1286I GDGCHC 3 cut(s) 287, 955, 1083
Bsp13I TCCGGA 2 cut(s) 279, 939
BspACI CCGC 7 cut(s) 155, 313, 412, 956, 1063, 1074, 1113
BspANI GGCC 3 cut(s) 141, 915, 1112
BspDI ATCGAT 2 cut(s) 87, 1156
BspEI TCCGGA 2 cut(s) 279, 939
BspFNI CGCG 1 cut(s) 1065
BspLI GGNNCC 1 cut(s) 833
BspMI ACCTGC 1 cut(s) 522
BspPI GGATC 2 cut(s) 86, 488
BspTI CTTAAG 2 cut(s) 47, 1118
BspTNI GGTCTC 1 cut(s) 498
BsrBI CCGCTC 2 cut(s) 958, 1115
BssECI CCNNGG 2 cut(s) 109, 1086
BssNI GRCGYC 1 cut(s) 926
BssT1I CCWWGG 1 cut(s) 1086
Bst2UI CCWGG 1 cut(s) 758
Bst4CI ACNGT 3 cut(s) 289, 519, 1049
Bst6I CTCTTC 2 cut(s) 452, 965
BstACI GRCGYC 1 cut(s) 926
BstAFI CTTAAG 2 cut(s) 47, 1118
BstC8I GCNNGC 2 cut(s) 508, 1125
BstF5I GGATG 4 cut(s) 120, 459, 753, 1208
BstFNI CGCG 1 cut(s) 1065
BstMAI GTCTC 4 cut(s) 191, 498, 917, 967
BstMCI CGRYCG 3 cut(s) 364, 651, 1113
BstMWI GCNNNNNNNGC 1 cut(s) 1022
BstNI CCWGG 1 cut(s) 758
BstSCI CCNGG 1 cut(s) 756
BstSFI CTRYAG 1 cut(s) 718
BstUI CGCG 1 cut(s) 1065
BstV2I GAAGAC 3 cut(s) 125, 261, 548
BstX2I RGATCY 2 cut(s) 133, 988
BstYI RGATCY 2 cut(s) 133, 988
BstZI CGGCCG 1 cut(s) 1110
Bsu15I ATCGAT 2 cut(s) 87, 1156
BsuRI GGCC 3 cut(s) 141, 915, 1112
BsuTUI ATCGAT 2 cut(s) 87, 1156
BtsCI GGATG 4 cut(s) 120, 459, 753, 1208
BtsIMutI CAGTG 1 cut(s) 1197
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 2 cut(s) 508, 1125
Cfr13I GGNCC 1 cut(s) 913
ClaI ATCGAT 2 cut(s) 87, 1156
CseI GACGC 2 cut(s) 542, 934
Csp6I GTAC 4 cut(s) 321, 358, 472, 520
CviQI GTAC 4 cut(s) 321, 358, 472, 520
EaeI YGGCCR 1 cut(s) 1110
EagI CGGCCG 1 cut(s) 1110
Eam1104I CTCTTC 2 cut(s) 452, 965
EarI CTCTTC 2 cut(s) 452, 965
EclXI CGGCCG 1 cut(s) 1110
Eco130I CCWWGG 1 cut(s) 1086
Eco24I GRGCYC 1 cut(s) 1083
Eco31I GGTCTC 1 cut(s) 498
Eco52I CGGCCG 1 cut(s) 1110
Eco57I CTGAAG 1 cut(s) 989
Eco88I CYCGRG 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 756
EcoT14I CCWWGG 1 cut(s) 1086
EcoT38I GRGCYC 1 cut(s) 1083
ErhI CCWWGG 1 cut(s) 1086
Esp3I CGTCTC 1 cut(s) 917
FaiI YATR 7 cut(s) 188, 485, 551, 720, 818, 869, 878
FauI CCCGC 1 cut(s) 1070
FbaI TGATCA 1 cut(s) 586
FblI GTMKAC 1 cut(s) 1220
Fnu4HI GCNGC 2 cut(s) 1074, 1113
FokI GGATG 4 cut(s) 127, 466, 740, 1195
FriOI GRGCYC 1 cut(s) 1083
Fsp4HI GCNGC 2 cut(s) 1074, 1113
FspBI CTAG 3 cut(s) 131, 855, 888
GluI GCNGC 2 cut(s) 1074, 1113
GsaI CCCAGC 1 cut(s) 492
GsuI CTGGAG 3 cut(s) 83, 194, 337
HaeIII GGCC 3 cut(s) 141, 915, 1112
HapII CCGG 3 cut(s) 280, 737, 940
HgaI GACGC 2 cut(s) 542, 934
Hin1I GRCGYC 1 cut(s) 926
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 7 cut(s) 309, 382, 680, 733, 746, 775, 892
HpaII CCGG 3 cut(s) 280, 737, 940
HphI GGTGA 2 cut(s) 1037, 1045
Hpy166II GTNNAC 3 cut(s) 292, 779, 1221
Hpy8I GTNNAC 3 cut(s) 292, 779, 1221
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 2 cut(s) 172, 421
HpyCH4III ACNGT 3 cut(s) 289, 519, 1049
HpyCH4IV ACGT 3 cut(s) 294, 391, 926
HpyCH4V TGCA 4 cut(s) 443, 467, 543, 964
HpyF10VI GCNNNNNNNGC 1 cut(s) 1022
HpySE526I ACGT 3 cut(s) 294, 391, 926
Hsp92I GRCGYC 1 cut(s) 926
Kpn2I TCCGGA 2 cut(s) 279, 939
Ksp22I TGATCA 1 cut(s) 586
LmnI GCTCC 5 cut(s) 282, 942, 942, 958, 963
LweI GCATC 1 cut(s) 430
MaeI CTAG 3 cut(s) 131, 855, 888
MaeII ACGT 3 cut(s) 294, 391, 926
MaeIII GTNAC 3 cut(s) 365, 513, 1192
MbiI CCGCTC 2 cut(s) 958, 1115
MboII GAAGA 9 cut(s) 130, 263, 266, 469, 548, 575, 982, 998, 1031
MflI RGATCY 2 cut(s) 133, 988
MhlI GDGCHC 3 cut(s) 287, 955, 1083
MluCI AATT 9 cut(s) 11, 35, 68, 275, 297, 375, 797, 880, 1198
MlyI GAGTC 2 cut(s) 689, 784
MmeI TCCRAC 1 cut(s) 672
MroI TCCGGA 2 cut(s) 279, 939
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 2 cut(s) 48, 1119
MslI CAYNNNNRTG 1 cut(s) 548
MspA1I CMGCKG 1 cut(s) 315
MspCI CTTAAG 2 cut(s) 47, 1118
MspI CCGG 3 cut(s) 280, 737, 940
MspR9I CCNGG 1 cut(s) 758
Mva1269I GAATGC 1 cut(s) 939
MvaI CCWGG 1 cut(s) 758
MvnI CGCG 1 cut(s) 1065
MwoI GCNNNNNNNGC 1 cut(s) 1022
NlaIV GGNNCC 1 cut(s) 833
NmeAIII GCCGAG 2 cut(s) 117, 1102
NmuCI GTSAC 2 cut(s) 365, 1192
PaeR7I CTCGAG 1 cut(s) 418
PcsI WCGNNNNNNNCGW 1 cut(s) 680
PctI GAATGC 1 cut(s) 939
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 5 cut(s) 309, 382, 733, 746, 892
PflMI CCANNNNNTGG 1 cut(s) 763
PkrI GCNGC 2 cut(s) 1075, 1114
Ple19I CGATCG 2 cut(s) 364, 651
PleI GAGTC 2 cut(s) 688, 783
PpsI GAGTC 2 cut(s) 688, 783
Psp6I CCWGG 1 cut(s) 756
PspFI CCCAGC 1 cut(s) 488
PspGI CCWGG 1 cut(s) 756
PspN4I GGNNCC 1 cut(s) 833
PspPI GGNCC 1 cut(s) 913
PsuI RGATCY 2 cut(s) 133, 988
PvuI CGATCG 2 cut(s) 364, 651
RsaI GTAC 4 cut(s) 322, 359, 473, 521
RsaNI GTAC 4 cut(s) 321, 358, 472, 520
RseI CAYNNNNRTG 1 cut(s) 548
SaqAI TTAA 2 cut(s) 48, 1119
SatI GCNGC 2 cut(s) 1074, 1113
Sau96I GGNCC 1 cut(s) 913
SchI GAGTC 2 cut(s) 689, 784
ScrFI CCNGG 1 cut(s) 758
SduI GDGCHC 3 cut(s) 287, 955, 1083
SfaNI GCATC 1 cut(s) 430
SfcI CTRYAG 1 cut(s) 718
Sfr274I CTCGAG 1 cut(s) 418
SlaI CTCGAG 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 5 cut(s) 47, 404, 418, 917, 1118
SmoI CTYRAG 5 cut(s) 47, 404, 418, 917, 1118
Sse9I AATT 9 cut(s) 11, 35, 68, 275, 297, 375, 797, 880, 1198
SsiI CCGC 7 cut(s) 155, 313, 412, 956, 1063, 1074, 1113
SspI AATATT 2 cut(s) 229, 663
SspMI CTAG 3 cut(s) 131, 855, 888
StyD4I CCNGG 1 cut(s) 756
StyI CCWWGG 1 cut(s) 1086
TaaI ACNGT 3 cut(s) 289, 519, 1049
TaiI ACGT 3 cut(s) 297, 394, 929
TaqI TCGA 5 cut(s) 24, 87, 419, 639, 1156
TasI AATT 9 cut(s) 11, 35, 68, 275, 297, 375, 797, 880, 1198
TauI GCSGC 2 cut(s) 1076, 1115
TfiI GAWTC 5 cut(s) 309, 382, 733, 746, 892
Tru1I TTAA 2 cut(s) 48, 1119
Tru9I TTAA 2 cut(s) 48, 1119
TscAI CASTG 1 cut(s) 1197
TseFI GTSAC 2 cut(s) 365, 1192
Tsp45I GTSAC 2 cut(s) 365, 1192
TspDTI ATGAA 7 cut(s) 131, 221, 470, 742, 810, 893, 1155
TspGWI ACGGA 4 cut(s) 137, 689, 824, 938
TspRI CASTG 1 cut(s) 1197
Van91I CCANNNNNTGG 1 cut(s) 763
Vha464I CTTAAG 2 cut(s) 47, 1118
XapI RAATTY 2 cut(s) 275, 880
XbaI TCTAGA 1 cut(s) 130
XhoI CTCGAG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 1220
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 3 cut(s) 131, 855, 888
ZraI GACGTC 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.