RLG00000023229

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000672
Physical Location & Seq
Reverse (-)
20201097 .. 20202163
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023229

Sequence Viewer

Length: 546 bp
ATGGCCGCCGCTGACCAAGAATCCAGTGTTGAAGCAAGGATGAACAGCATCACCGAAACACGAGAGTTTGCAGTCCGAGTTCATCACAATAGAGCAAACTACTACCGCAGCAGTTCCGGCGTGGACATACTTCCGATCAGATTCAAATGGGGCACGGTAACTGCAAACTACAATCCAAGTATCCAAGGTTATGATTGTGAACCTCCAGATATTTTATCCAGACGTACTCTTAAGCTGCGGTTCTCGAGGGAGAGGGCGAGTGGGAGCGCAAAGATAACGGAGAGGAGGTTGGCCAAGTACAAAAGGGTCATAGCTAGGTCTCCGACGGAAATGGACAAGTTACAGATGAAGCTGGCTAGGTTTCTGCGCAGCCCTGTGGGTATTCCCGAAGATAAGCACCCGTTTATTATTGTACATATATTGGATATGATTGAATTAGCCGACCCAGATAAAATTATCGATGTACACTTGATGGACATAACTGTTAGAGCTGCGGGGGAATTCGCGCACTTAAACGGCCAGAAGAATCAGTATGTTATTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

182

Amino Acids

20.74

Weight (kDa)

9.41

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 368
AccII CGCG 1 cut(s) 506
AciI CCGC 5 cut(s) 6, 9, 106, 238, 494
AcoI YGGCCR 3 cut(s) 3, 291, 517
AcsI RAATTY 1 cut(s) 500
AfaI GTAC 4 cut(s) 226, 299, 414, 465
AflII CTTAAG 1 cut(s) 230
AgsI TTSAA 3 cut(s) 32, 145, 434
AluBI AGCT 4 cut(s) 235, 314, 352, 491
AluI AGCT 4 cut(s) 235, 314, 352, 491
Alw26I GTCTC 1 cut(s) 324
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 3 cut(s) 3, 291, 517
ApeKI GCWGC 4 cut(s) 108, 235, 369, 491
ApoI RAATTY 1 cut(s) 500
AspLEI GCGC 3 cut(s) 269, 369, 508
AsuHPI GGTGA 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 244
BaeGI GKGCMC 1 cut(s) 155
BalI TGGCCA 1 cut(s) 293
BauI CACGAG 1 cut(s) 60
BbvI GCAGC 4 cut(s) 120, 222, 381, 478
BccI CCATC 1 cut(s) 466
BceAI ACGGC 1 cut(s) 532
BciVI GTATCC 1 cut(s) 191
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 2 cut(s) 315, 357
BfrI CTTAAG 1 cut(s) 230
BfuI GTATCC 1 cut(s) 191
BisI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 492
BlsI GCNGC 6 cut(s) 7, 10, 110, 237, 371, 493
BmeT110I CYCGRG 1 cut(s) 244
BmsI GCATC 1 cut(s) 57
BpmI CTGGAG 1 cut(s) 189
Bsa29I ATCGAT 1 cut(s) 459
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 184
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bse1I ACTGG 1 cut(s) 24
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 184
BseGI GGATG 1 cut(s) 45
BseNI ACTGG 1 cut(s) 24
BseRI GAGGAG 1 cut(s) 298
BseSI GKGCMC 1 cut(s) 155
BseXI GCAGC 4 cut(s) 120, 222, 381, 478
Bsh1236I CGCG 1 cut(s) 506
BshFI GGCC 3 cut(s) 5, 293, 519
BshVI ATCGAT 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 1 cut(s) 117
BsmAI GTCTC 1 cut(s) 324
BsnI GGCC 3 cut(s) 5, 293, 519
Bso31I GGTCTC 1 cut(s) 324
BsoBI CYCGRG 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 155
Bsp1407I TGTACA 2 cut(s) 412, 463
Bsp143I GATC 1 cut(s) 135
BspACI CCGC 5 cut(s) 6, 9, 106, 238, 494
BspANI GGCC 3 cut(s) 5, 293, 519
BspDI ATCGAT 1 cut(s) 459
BspFNI CGCG 1 cut(s) 506
BspTI CTTAAG 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 324
BsrGI TGTACA 2 cut(s) 412, 463
BsrI ACTGG 1 cut(s) 24
BssECI CCNNGG 1 cut(s) 184
BssMI GATC 1 cut(s) 135
BssSI CACGAG 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 184
Bst2BI CACGAG 1 cut(s) 60
Bst4CI ACNGT 2 cut(s) 157, 484
BstAFI CTTAAG 1 cut(s) 230
BstAUI TGTACA 2 cut(s) 412, 463
BstC8I GCNNGC 1 cut(s) 354
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 1 cut(s) 506
BstHHI GCGC 3 cut(s) 269, 369, 508
BstKTI GATC 1 cut(s) 138
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 1 cut(s) 135
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstSLI GKGCMC 1 cut(s) 155
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 4 cut(s) 120, 222, 381, 478
Bsu15I ATCGAT 1 cut(s) 459
BsuI GTATCC 1 cut(s) 191
BsuRI GGCC 3 cut(s) 5, 293, 519
BsuTUI ATCGAT 1 cut(s) 459
BtsCI GGATG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 354
CfoI GCGC 3 cut(s) 269, 369, 508
ClaI ATCGAT 1 cut(s) 459
Csp6I GTAC 4 cut(s) 225, 298, 413, 464
CviQI GTAC 4 cut(s) 225, 298, 413, 464
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
EaeI YGGCCR 3 cut(s) 3, 291, 517
Eco130I CCWWGG 1 cut(s) 184
Eco31I GGTCTC 1 cut(s) 324
Eco88I CYCGRG 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 500
EcoT14I CCWWGG 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 184
FaiI YATR 8 cut(s) 128, 192, 311, 417, 419, 428, 479, 534
FauI CCCGC 1 cut(s) 487
Fnu4HI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 492
FokI GGATG 1 cut(s) 52
Fsp4HI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 492
FspBI CTAG 2 cut(s) 315, 357
FspI TGCGCA 1 cut(s) 368
GlaI GCGC 3 cut(s) 268, 368, 507
GluI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 492
GsuI CTGGAG 1 cut(s) 189
HaeIII GGCC 3 cut(s) 5, 293, 519
HapII CCGG 1 cut(s) 117
HhaI GCGC 3 cut(s) 269, 369, 508
Hin6I GCGC 3 cut(s) 267, 367, 506
HinP1I GCGC 3 cut(s) 267, 367, 506
HinfI GANTC 3 cut(s) 20, 141, 526
HpaII CCGG 1 cut(s) 117
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 3 cut(s) 124, 200, 466
Hpy188I TCNGA 4 cut(s) 77, 135, 140, 324
Hpy188III TCNNGA 4 cut(s) 206, 219, 244, 386
Hpy8I GTNNAC 3 cut(s) 124, 200, 466
Hpy99I CGWCG 1 cut(s) 328
HpyCH4III ACNGT 2 cut(s) 157, 484
HpyCH4IV ACGT 1 cut(s) 223
HpyCH4V TGCA 2 cut(s) 71, 164
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpySE526I ACGT 1 cut(s) 223
HspAI GCGC 3 cut(s) 267, 367, 506
Kzo9I GATC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 264
LpnPI CCDG 8 cut(s) 37, 130, 219, 232, 338, 387, 459, 533
Lsp1109I GCAGC 4 cut(s) 120, 222, 381, 478
LweI GCATC 1 cut(s) 57
MaeI CTAG 2 cut(s) 315, 357
MaeII ACGT 1 cut(s) 223
MaeIII GTNAC 2 cut(s) 157, 339
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MboII GAAGA 2 cut(s) 401, 535
MhlI GDGCHC 1 cut(s) 155
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 3 cut(s) 434, 453, 500
MluNI TGGCCA 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 347
MnlI CCTC 5 cut(s) 213, 240, 246, 276, 279
Mox20I TGGCCA 1 cut(s) 293
MscI TGGCCA 1 cut(s) 293
MseI TTAA 2 cut(s) 231, 512
Msp20I TGGCCA 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 11
MspCI CTTAAG 1 cut(s) 230
MspI CCGG 1 cut(s) 117
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 1 cut(s) 135
NsbI TGCGCA 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 244
PfeI GAWTC 3 cut(s) 20, 141, 526
PkrI GCNGC 6 cut(s) 7, 10, 110, 237, 371, 493
RsaI GTAC 4 cut(s) 226, 299, 414, 465
RsaNI GTAC 4 cut(s) 225, 298, 413, 464
SaqAI TTAA 2 cut(s) 231, 512
SatI GCNGC 6 cut(s) 6, 9, 109, 236, 370, 492
Sau3AI GATC 1 cut(s) 135
SduI GDGCHC 1 cut(s) 155
SfaNI GCATC 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 244
SlaI CTCGAG 1 cut(s) 244
SmlI CTYRAG 2 cut(s) 230, 244
SmoI CTYRAG 2 cut(s) 230, 244
Sse9I AATT 3 cut(s) 434, 453, 500
SsiI CCGC 5 cut(s) 6, 9, 106, 238, 494
SspMI CTAG 2 cut(s) 315, 357
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 2 cut(s) 157, 484
TaiI ACGT 1 cut(s) 226
TaqI TCGA 2 cut(s) 245, 459
TasI AATT 3 cut(s) 434, 453, 500
TatI WGTACW 3 cut(s) 297, 412, 463
TauI GCSGC 2 cut(s) 8, 11
TfiI GAWTC 3 cut(s) 20, 141, 526
Tru1I TTAA 2 cut(s) 231, 512
Tru9I TTAA 2 cut(s) 231, 512
TscAI CASTG 1 cut(s) 31
TseI GCWGC 4 cut(s) 108, 235, 369, 491
TspDTI ATGAA 3 cut(s) 56, 71, 362
TspGWI ACGGA 2 cut(s) 293, 341
TspRI CASTG 1 cut(s) 31
Vha464I CTTAAG 1 cut(s) 230
XapI RAATTY 1 cut(s) 500
XhoI CTCGAG 1 cut(s) 244
XspI CTAG 2 cut(s) 315, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.