RchiOBHm_Chr6g0305991

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000672
Physical Location & Seq
Reverse (-)
64783225 .. 64784113
889 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27501

Sequence Viewer

Length: 588 bp
ATGGATAAGTTGGTAGACAAGCTGGCAAGGTATCTTTCTGATACTGTGGGTGTTGAAGAGGATCAACACCCTTGTATTATTCTGAAAACGGTGGAGATGATTCAAGCCGCTGTCCACGACCGGGTTATCGATGTGCAGATATCGGACATCACATTTATCGAACAGGAACCTGAACCGGAGGAGGAATCTGAGCTGGAGGAGTCTGAACCGGAGGAGGAGTCGGAACAAGAGGAGGAGTCTGAAACGGAGGAGGATTCTGAACAGGAGGGATTCGAGGATTTGGACGACACATCGTATGGGGACAATCTGACTCCTGCATCTGAATCATTCATGGAGGATTTGAAGAAATTGAGAGTACTTGGTTCGGAATTGGAAATGCTTGGGCAGACGCCAAACTGTGCTATTTGTCTGGAGGTGCTTGCTGAAGAAGGATATGATGATCAACTGATCACCATTACTCGTTTGCCTTGCTTACACTATTATCATCGGGATTGCATTATCAAGTGGATGATGATCAATAATTTATGCCCAGTTTGTCGACGCCAGAATGTGGAAGCAGGGGAGATAAGAAATAACTTACCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

195

Amino Acids

22.4

Weight (kDa)

4.06

Isoelectric Point (pI)

70.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 132 - 180 2e-11 Ring finger domain
zf-rbx1 PF12678 132 - 180 8.5e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 133 - 179 6.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 133 - 179 4.2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 550
AccI GTMKAC 2 cut(s) 15, 538
AciI CCGC 1 cut(s) 108
AclWI GGATC 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 444
AcyI GRCGYC 2 cut(s) 389, 541
AfaI GTAC 1 cut(s) 357
AfiI CCNNNNNNNGG 2 cut(s) 121, 550
AgsI TTSAA 3 cut(s) 56, 104, 343
AluBI AGCT 3 cut(s) 22, 193, 585
AluI AGCT 3 cut(s) 22, 193, 585
AlwI GGATC 1 cut(s) 69
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 1 cut(s) 442
BclI TGATCA 3 cut(s) 439, 447, 513
BcnI CCSGG 1 cut(s) 122
BfaI CTAG 2 cut(s) 582, 586
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
BmcAI AGTACT 1 cut(s) 357
Bme1390I CCNGG 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 122
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 1 cut(s) 326
BmuI ACTGGG 1 cut(s) 524
BpmI CTGGAG 2 cut(s) 215, 431
BpuMI CCSGG 1 cut(s) 122
Bsa29I ATCGAT 1 cut(s) 129
BsaBI GATNNNNATC 1 cut(s) 512
BsaHI GRCGYC 2 cut(s) 389, 541
BsaWI WCCGGW 2 cut(s) 175, 208
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 550
Bse1I ACTGG 1 cut(s) 530
Bse8I GATNNNNATC 1 cut(s) 512
BseCI ATCGAT 1 cut(s) 129
BseGI GGATG 1 cut(s) 513
BseJI GATNNNNATC 1 cut(s) 512
BseLI CCNNNNNNNGG 2 cut(s) 121, 550
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 1 cut(s) 530
BseRI GAGGAG 7 cut(s) 194, 212, 227, 230, 245, 248, 263
BsgI GTGCAG 1 cut(s) 155
Bsh1285I CGRYCG 1 cut(s) 121
BshVI ATCGAT 1 cut(s) 129
BsiEI CGRYCG 1 cut(s) 121
BsiSI CCGG 3 cut(s) 121, 176, 209
BslFI GGGAC 1 cut(s) 314
BslI CCNNNNNNNGG 2 cut(s) 121, 550
BsmFI GGGAC 1 cut(s) 314
Bsp143I GATC 4 cut(s) 61, 439, 447, 513
BspACI CCGC 1 cut(s) 108
BspCNI CTCAG 1 cut(s) 181
BspDI ATCGAT 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 1 cut(s) 69
BsrI ACTGG 1 cut(s) 530
BssMI GATC 4 cut(s) 61, 439, 447, 513
BssNI GRCGYC 2 cut(s) 389, 541
Bst4CI ACNGT 3 cut(s) 46, 91, 398
Bst6I CTCTTC 1 cut(s) 51
BstACI GRCGYC 2 cut(s) 389, 541
BstC8I GCNNGC 2 cut(s) 24, 420
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 513
BstKTI GATC 4 cut(s) 64, 442, 450, 516
BstMBI GATC 4 cut(s) 61, 439, 447, 513
BstMCI CGRYCG 1 cut(s) 121
BstSCI CCNGG 1 cut(s) 120
Bsu15I ATCGAT 1 cut(s) 129
BsuTUI ATCGAT 1 cut(s) 129
BtsCI GGATG 1 cut(s) 513
Cac8I GCNNGC 2 cut(s) 24, 420
ClaI ATCGAT 1 cut(s) 129
CseI GACGC 2 cut(s) 397, 549
Csp6I GTAC 1 cut(s) 356
CviAII CATG 1 cut(s) 331
CviJI RGCY 4 cut(s) 22, 107, 193, 585
CviKI_1 RGCY 4 cut(s) 22, 107, 193, 585
CviQI GTAC 1 cut(s) 356
DdeI CTNAG 1 cut(s) 189
DpnI GATC 4 cut(s) 63, 441, 449, 515
DpnII GATC 4 cut(s) 61, 439, 447, 513
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco32I GATATC 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 444
EcoRV GATATC 1 cut(s) 141
FaeI CATG 1 cut(s) 334
FaiI YATR 4 cut(s) 297, 332, 435, 526
FaqI GGGAC 1 cut(s) 314
FatI CATG 1 cut(s) 330
FbaI TGATCA 3 cut(s) 439, 447, 513
FblI GTMKAC 2 cut(s) 15, 538
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 1 cut(s) 520
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 2 cut(s) 582, 586
GluI GCNGC 1 cut(s) 108
GsuI CTGGAG 2 cut(s) 215, 431
HapII CCGG 3 cut(s) 121, 176, 209
HgaI GACGC 2 cut(s) 397, 549
Hin1I GRCGYC 2 cut(s) 389, 541
Hin1II CATG 1 cut(s) 334
HincII GTYRAC 1 cut(s) 539
HindII GTYRAC 1 cut(s) 539
HinfI GANTC 9 cut(s) 100, 185, 200, 218, 236, 254, 270, 310, 323
HpaII CCGG 3 cut(s) 121, 176, 209
HphI GGTGA 1 cut(s) 442
Hpy166II GTNNAC 3 cut(s) 16, 115, 539
Hpy188III TCNNGA 2 cut(s) 410, 488
Hpy8I GTNNAC 3 cut(s) 16, 115, 539
Hpy99I CGWCG 1 cut(s) 543
HpyAV CCTTC 1 cut(s) 422
HpyCH4III ACNGT 3 cut(s) 46, 91, 398
HpyCH4V TGCA 3 cut(s) 136, 317, 495
HpyF3I CTNAG 1 cut(s) 189
Hsp92I GRCGYC 2 cut(s) 389, 541
Hsp92II CATG 1 cut(s) 334
Ksp22I TGATCA 3 cut(s) 439, 447, 513
Kzo9I GATC 4 cut(s) 61, 439, 447, 513
LweI GCATC 1 cut(s) 326
MaeI CTAG 2 cut(s) 582, 586
MalI GATC 4 cut(s) 63, 441, 449, 515
MboI GATC 4 cut(s) 61, 439, 447, 513
MboII GAAGA 3 cut(s) 68, 355, 437
MluCI AATT 3 cut(s) 347, 368, 520
MlyI GAGTC 4 cut(s) 209, 227, 245, 304
MmeI TCCRAC 1 cut(s) 201
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 3 cut(s) 121, 176, 209
MspR9I CCNGG 1 cut(s) 122
NciI CCSGG 1 cut(s) 122
NdeII GATC 4 cut(s) 61, 439, 447, 513
NlaIII CATG 1 cut(s) 334
NlaIV GGNNCC 1 cut(s) 168
PfeI GAWTC 5 cut(s) 100, 185, 254, 270, 323
PflMI CCANNNNNTGG 1 cut(s) 550
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 4 cut(s) 208, 226, 244, 304
PpsI GAGTC 4 cut(s) 208, 226, 244, 304
PspN4I GGNNCC 1 cut(s) 168
RsaI GTAC 1 cut(s) 357
RsaNI GTAC 1 cut(s) 356
SalI GTCGAC 1 cut(s) 537
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 4 cut(s) 61, 439, 447, 513
ScaI AGTACT 1 cut(s) 357
SchI GAGTC 4 cut(s) 209, 227, 245, 304
ScrFI CCNGG 1 cut(s) 122
SetI ASST 7 cut(s) 24, 32, 172, 195, 417, 583, 587
SfaNI GCATC 1 cut(s) 326
Sse9I AATT 3 cut(s) 347, 368, 520
SsiI CCGC 1 cut(s) 108
SspMI CTAG 2 cut(s) 582, 586
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 3 cut(s) 46, 91, 398
TaqI TCGA 4 cut(s) 129, 159, 273, 538
TasI AATT 3 cut(s) 347, 368, 520
TatI WGTACW 1 cut(s) 355
TauI GCSGC 1 cut(s) 110
TfiI GAWTC 5 cut(s) 100, 185, 254, 270, 323
TspDTI ATGAA 1 cut(s) 319
TspGWI ACGGA 1 cut(s) 260
Van91I CCANNNNNTGG 1 cut(s) 550
XmiI GTMKAC 2 cut(s) 15, 538
XspI CTAG 2 cut(s) 582, 586
ZrmI AGTACT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.