Rorug06G0352200

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000672
Physical Location & Seq
Reverse (-)
50132004 .. 50133036
1033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0352200.1

Sequence Viewer

Length: 423 bp
ATGAGCAAATACGGTGCTTTTTTAGCCGCTACGGGTCAAGGTGGAATCGAGGCAATCATCAGAGATGAGTTTGGTGTGTGTTTAGCTGCAATTGCACGCCCTTTTCCCCAGGTTAGATCTGCATTCCATATGGAGTGTGAGGCTATGAGAGCAGGACTCCTTCTAATTATTCACCAAGGCATGACAGAGGTCGACATTGAGACCGATTGTGTTGCGGTGGTTAATGCACTTAATGGAACCATGGAGGATTTCTTTGAGAAGAACTCAGGAAGATACGCGTACGATGCCAGACCACCGCCAACAGCCGTGCTCAGACCCATACTTGATCGGAACCCGACCTTCATCTACATCGACGACCTGAATTCGGAATCGTCCTCCGACACCCCAGAACCTGACTTAGGGTCCCGCGTCAAGGCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

140

Amino Acids

15.32

Weight (kDa)

4.63

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 5 - 82 9.8e-13 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 192
AccII CGCG 2 cut(s) 278, 408
AciI CCGC 4 cut(s) 27, 215, 296, 406
AcsI RAATTY 1 cut(s) 361
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 3 cut(s) 364, 398, 412
AflIII ACRYGT 1 cut(s) 276
AhdI GACNNNNNGTC 1 cut(s) 400
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
Alw21I GWGCWC 1 cut(s) 312
Alw26I GTCTC 1 cut(s) 194
AlwNI CAGNNNCTG 1 cut(s) 392
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 361
AspS9I GGNCC 1 cut(s) 402
AsuHPI GGTGA 1 cut(s) 164
AvaII GGWCC 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 290
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 1 cut(s) 421
BglII AGATCT 1 cut(s) 116
BisI GCNGC 2 cut(s) 27, 87
BlsI GCNGC 2 cut(s) 28, 88
Bme1390I CCNGG 1 cut(s) 110
Bme18I GGWCC 1 cut(s) 402
BmeRI GACNNNNNGTC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 402
BmiI GGNNCC 4 cut(s) 238, 332, 403, 404
BmrFI CCNGG 1 cut(s) 110
BmsI GCATC 1 cut(s) 274
BoxI GACNNNNGTC 1 cut(s) 188
BplI GAGNNNNNCTC 4 cut(s) 141, 173, 248, 280
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 3 cut(s) 108, 175, 240
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 398, 412
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 3 cut(s) 108, 175, 240
BseLI CCNNNNNNNGG 3 cut(s) 364, 398, 412
BseMII CTCAG 2 cut(s) 279, 325
BseXI GCAGC 1 cut(s) 73
Bsh1236I CGCG 2 cut(s) 278, 408
BsiHKAI GWGCWC 1 cut(s) 312
BsiWI CGTACG 1 cut(s) 279
BslFI GGGAC 1 cut(s) 388
BslI CCNNNNNNNGG 3 cut(s) 364, 398, 412
BsmAI GTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 388
BsmI GAATGC 1 cut(s) 122
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 2 cut(s) 116, 325
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 4 cut(s) 27, 215, 296, 406
BspCNI CTCAG 2 cut(s) 278, 324
BspFNI CGCG 2 cut(s) 278, 408
BspLI GGNNCC 4 cut(s) 238, 332, 403, 404
BspTNI GGTCTC 1 cut(s) 194
BssECI CCNNGG 3 cut(s) 108, 175, 240
BssMI GATC 2 cut(s) 116, 325
BssT1I CCWWGG 2 cut(s) 175, 240
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 3 cut(s) 265, 311, 397
BstDSI CCRYGG 1 cut(s) 240
BstENI CCTNNNNNAGG 1 cut(s) 396
BstFNI CGCG 2 cut(s) 278, 408
BstKTI GATC 2 cut(s) 119, 328
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 2 cut(s) 116, 325
BstMWI GCNNNNNNNGC 4 cut(s) 23, 92, 149, 284
BstNI CCWGG 1 cut(s) 110
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 108
BstUI CGCG 2 cut(s) 278, 408
BstV1I GCAGC 1 cut(s) 73
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BtgI CCRYGG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 97
CaiI CAGNNNCTG 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 402
CseI GACGC 1 cut(s) 397
Csp6I GTAC 1 cut(s) 280
CviAII CATG 2 cut(s) 181, 241
CviJI RGCY 5 cut(s) 26, 86, 143, 305, 416
CviKI_1 RGCY 5 cut(s) 26, 86, 143, 305, 416
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 3 cut(s) 265, 311, 397
DpnI GATC 2 cut(s) 118, 327
DpnII GATC 2 cut(s) 116, 325
DriI GACNNNNNGTC 1 cut(s) 400
Eam1105I GACNNNNNGTC 1 cut(s) 400
Eco130I CCWWGG 2 cut(s) 175, 240
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 1 cut(s) 108
EcoT14I CCWWGG 2 cut(s) 175, 240
ErhI CCWWGG 2 cut(s) 175, 240
FaeI CATG 2 cut(s) 184, 244
FaiI YATR 6 cut(s) 129, 131, 146, 182, 242, 320
FaqI GGGAC 1 cut(s) 388
FatI CATG 2 cut(s) 180, 240
FauI CCCGC 1 cut(s) 413
FauNDI CATATG 1 cut(s) 129
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 2 cut(s) 27, 87
Fsp4HI GCNGC 2 cut(s) 27, 87
FspBI CTAG 1 cut(s) 421
GluI GCNGC 2 cut(s) 27, 87
HgaI GACGC 1 cut(s) 397
Hin1II CATG 2 cut(s) 184, 244
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 3 cut(s) 45, 156, 368
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 5 cut(s) 62, 314, 330, 367, 379
Hpy188III TCNNGA 1 cut(s) 267
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 1 cut(s) 356
HpyAV CCTTC 2 cut(s) 170, 349
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4V TGCA 4 cut(s) 89, 95, 122, 227
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 92, 149, 284
HpyF3I CTNAG 3 cut(s) 265, 311, 397
Hsp92II CATG 2 cut(s) 184, 244
KflI GGGWCCC 1 cut(s) 402
Kzo9I GATC 2 cut(s) 116, 325
LpnPI CCDG 8 cut(s) 95, 122, 138, 252, 301, 371, 399, 405
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 1 cut(s) 274
MaeI CTAG 1 cut(s) 421
MalI GATC 2 cut(s) 118, 327
MboI GATC 2 cut(s) 116, 325
MboII GAAGA 2 cut(s) 271, 282
MfeI CAATTG 1 cut(s) 90
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 3 cut(s) 90, 165, 361
MluI ACGCGT 1 cut(s) 276
MlyI GAGTC 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 5 cut(s) 43, 133, 181, 238, 385
MseI TTAA 2 cut(s) 222, 231
MspR9I CCNGG 1 cut(s) 110
MunI CAATTG 1 cut(s) 90
Mva1269I GAATGC 1 cut(s) 122
MvaI CCWGG 1 cut(s) 110
MvnI CGCG 2 cut(s) 278, 408
MwoI GCNNNNNNNGC 4 cut(s) 23, 92, 149, 284
NcoI CCATGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 129
NdeII GATC 2 cut(s) 116, 325
NlaIII CATG 2 cut(s) 184, 244
NlaIV GGNNCC 4 cut(s) 238, 332, 403, 404
PctI GAATGC 1 cut(s) 122
PfeI GAWTC 2 cut(s) 45, 368
Pfl23II CGTACG 1 cut(s) 279
PkrI GCNGC 2 cut(s) 28, 88
PleI GAGTC 1 cut(s) 150
PpsI GAGTC 1 cut(s) 150
PpuMI RGGWCCY 1 cut(s) 402
PshAI GACNNNNGTC 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspLI CGTACG 1 cut(s) 279
PspN4I GGNNCC 4 cut(s) 238, 332, 403, 404
PspPI GGNCC 1 cut(s) 402
PspPPI RGGWCCY 1 cut(s) 402
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SalI GTCGAC 1 cut(s) 191
SaqAI TTAA 2 cut(s) 222, 231
SatI GCNGC 2 cut(s) 27, 87
Sau3AI GATC 2 cut(s) 116, 325
Sau96I GGNCC 1 cut(s) 402
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 1 cut(s) 110
SduI GDGCHC 1 cut(s) 312
SetI ASST 7 cut(s) 43, 88, 114, 192, 341, 360, 394
SfaNI GCATC 1 cut(s) 274
SinI GGWCC 1 cut(s) 402
Sse9I AATT 3 cut(s) 90, 165, 361
SsiI CCGC 4 cut(s) 27, 215, 296, 406
SspMI CTAG 1 cut(s) 421
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 2 cut(s) 175, 240
TaaI ACNGT 1 cut(s) 14
TaqI TCGA 3 cut(s) 48, 192, 351
TaqII GACCGA 1 cut(s) 218
TasI AATT 3 cut(s) 90, 165, 361
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 2 cut(s) 45, 368
Tru1I TTAA 2 cut(s) 222, 231
Tru9I TTAA 2 cut(s) 222, 231
TseI GCWGC 1 cut(s) 86
TspDTI ATGAA 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 402
XagI CCTNNNNNAGG 1 cut(s) 396
XapI RAATTY 1 cut(s) 361
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.