FvH4_7g27701

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000672
Physical Location & Seq
Reverse (-)
20658986 .. 20659372
387 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27701.t1

Sequence Viewer

Length: 387 bp
ATGAAGGAAGAGCTCAGAGATCTTCCGAAGGCTTACCATGGCTTCAGCTTTATCGAAGAAATGATGGAATCAGAGGAGAAAGAGAAGATCGAGAGAGAGAGAGGAGGTGGACCACGTCATCGGAATCGGTTTGACCTTCGCGTTGATGAGATGCAGTCGAGGCCGAAGCGGAGGTCGTCGAGGAGTCTTCGACGGTGGATCCAGAAGTCATCGTCGAGGTGGCGGCAACTTTGTCACGGTGGCGGCGAAATCGAAGTGGAAGAATGGATAGAGAGAGAAGGTGATATATGGTTTCCCATCCCGGTCTTCTTCTCCGCCGCCGACGACTGGATTGCCATGAAGGAGGTGCGAACCACGGCGGACGACGCTTGTGGAGCTCTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

129

Amino Acids

15.23

Weight (kDa)

5.99

Isoelectric Point (pI)

94.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 141
AciI CCGC 6 cut(s) 169, 223, 243, 315, 318, 359
AclWI GGATC 2 cut(s) 193, 206
AcuI CTGAAG 1 cut(s) 28
AfiI CCNNNNNNNGG 1 cut(s) 327
AjiI CACGTC 1 cut(s) 116
AluBI AGCT 3 cut(s) 13, 48, 377
AluI AGCT 3 cut(s) 13, 48, 377
Alw21I GWGCWC 2 cut(s) 15, 379
AlwI GGATC 2 cut(s) 193, 206
AoxI GGCC 1 cut(s) 161
ArsI GACNNNNNNTTYG 2 cut(s) 158, 190
AspS9I GGNCC 1 cut(s) 110
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 293
AvaII GGWCC 1 cut(s) 110
BamHI GGATCC 1 cut(s) 198
BanII GRGCYC 2 cut(s) 15, 379
BbsI GAAGAC 2 cut(s) 179, 298
Bbv12I GWGCWC 2 cut(s) 15, 379
BccI CCATC 2 cut(s) 58, 305
BceAI ACGGC 1 cut(s) 372
BcgI CGANNNNNNTGC 2 cut(s) 314, 348
BcnI CCSGG 1 cut(s) 302
BglII AGATCT 1 cut(s) 19
BisI GCNGC 3 cut(s) 224, 244, 318
BlsI GCNGC 3 cut(s) 225, 245, 319
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 1 cut(s) 110
BmgBI CACGTC 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 141
BpiI GAAGAC 2 cut(s) 179, 298
BpuMI CCSGG 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 37, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 327
Bse1I ACTGG 1 cut(s) 332
BseDI CCNNGG 2 cut(s) 37, 354
BseGI GGATG 1 cut(s) 297
BseLI CCNNNNNNNGG 1 cut(s) 327
BseMII CTCAG 1 cut(s) 28
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 3 cut(s) 89, 117, 196
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 1 cut(s) 163
BsiHKAI GWGCWC 2 cut(s) 15, 379
BsiSI CCGG 1 cut(s) 302
BslI CCNNNNNNNGG 1 cut(s) 327
BsnI GGCC 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 15, 379
Bsp143I GATC 3 cut(s) 19, 87, 198
Bsp19I CCATGG 1 cut(s) 37
BspACI CCGC 6 cut(s) 169, 223, 243, 315, 318, 359
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 1 cut(s) 27
BspFNI CGCG 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 200
BspPI GGATC 2 cut(s) 193, 206
BspQI GCTCTTC 1 cut(s) 3
BsrI ACTGG 1 cut(s) 332
BssECI CCNNGG 2 cut(s) 37, 354
BssMI GATC 3 cut(s) 19, 87, 198
BssT1I CCWWGG 1 cut(s) 37
Bst4CI ACNGT 2 cut(s) 195, 239
Bst6I CTCTTC 1 cut(s) 3
BstDEI CTNAG 1 cut(s) 14
BstDSI CCRYGG 2 cut(s) 37, 354
BstF5I GGATG 1 cut(s) 297
BstFNI CGCG 1 cut(s) 141
BstKTI GATC 3 cut(s) 22, 90, 201
BstMBI GATC 3 cut(s) 19, 87, 198
BstMWI GCNNNNNNNGC 3 cut(s) 160, 365, 374
BstSCI CCNGG 1 cut(s) 300
BstUI CGCG 1 cut(s) 141
BstV2I GAAGAC 2 cut(s) 179, 298
BstX2I RGATCY 2 cut(s) 19, 198
BstYI RGATCY 2 cut(s) 19, 198
BsuRI GGCC 1 cut(s) 163
BtgI CCRYGG 2 cut(s) 37, 354
BtrI CACGTC 1 cut(s) 116
BtsCI GGATG 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 110
CseI GACGC 1 cut(s) 374
CviAII CATG 2 cut(s) 38, 337
CviJI RGCY 6 cut(s) 13, 32, 42, 48, 163, 377
CviKI_1 RGCY 6 cut(s) 13, 32, 42, 48, 163, 377
DdeI CTNAG 1 cut(s) 14
DpnI GATC 3 cut(s) 21, 89, 200
DpnII GATC 3 cut(s) 19, 87, 198
Eam1104I CTCTTC 1 cut(s) 3
EarI CTCTTC 1 cut(s) 3
EciI GGCGGA 2 cut(s) 304, 374
Ecl136II GAGCTC 2 cut(s) 13, 377
Eco130I CCWWGG 1 cut(s) 37
Eco24I GRGCYC 2 cut(s) 15, 379
Eco47I GGWCC 1 cut(s) 110
Eco53kI GAGCTC 2 cut(s) 13, 377
Eco57I CTGAAG 1 cut(s) 28
EcoICRI GAGCTC 2 cut(s) 13, 377
EcoT14I CCWWGG 1 cut(s) 37
EcoT38I GRGCYC 2 cut(s) 15, 379
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 2 cut(s) 41, 340
FaiI YATR 4 cut(s) 39, 287, 289, 338
FatI CATG 2 cut(s) 37, 336
Fnu4HI GCNGC 3 cut(s) 224, 244, 318
FokI GGATG 1 cut(s) 284
FriOI GRGCYC 2 cut(s) 15, 379
Fsp4HI GCNGC 3 cut(s) 224, 244, 318
GluI GCNGC 3 cut(s) 224, 244, 318
HaeIII GGCC 1 cut(s) 163
HapII CCGG 1 cut(s) 302
HgaI GACGC 1 cut(s) 374
Hin1II CATG 2 cut(s) 41, 340
HinfI GANTC 3 cut(s) 68, 124, 184
HpaII CCGG 1 cut(s) 302
HphI GGTGA 1 cut(s) 293
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 4 cut(s) 17, 27, 73, 123
Hpy188III TCNNGA 2 cut(s) 91, 202
Hpy8I GTNNAC 1 cut(s) 110
Hpy99I CGWCG 5 cut(s) 181, 195, 217, 326, 368
HpyAV CCTTC 4 cut(s) 22, 146, 272, 334
HpyCH4III ACNGT 2 cut(s) 195, 239
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 1 cut(s) 154
HpyF10VI GCNNNNNNNGC 3 cut(s) 160, 365, 374
HpyF3I CTNAG 1 cut(s) 14
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 2 cut(s) 41, 340
Kzo9I GATC 3 cut(s) 19, 87, 198
LguI GCTCTTC 1 cut(s) 3
LmnI GCTCC 1 cut(s) 374
LpnPI CCDG 3 cut(s) 215, 313, 315
LweI GCATC 1 cut(s) 141
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 1 cut(s) 233
MalI GATC 3 cut(s) 21, 89, 200
MboI GATC 3 cut(s) 19, 87, 198
MboII GAAGA 8 cut(s) 14, 20, 68, 97, 179, 272, 298, 301
MflI RGATCY 2 cut(s) 19, 198
MhlI GDGCHC 2 cut(s) 15, 379
MlyI GAGTC 1 cut(s) 193
MnlI CCTC 8 cut(s) 67, 95, 98, 153, 165, 174, 210, 337
MspI CCGG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 302
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 3 cut(s) 160, 365, 374
NciI CCSGG 1 cut(s) 302
NcoI CCATGG 1 cut(s) 37
NdeII GATC 3 cut(s) 19, 87, 198
NlaIII CATG 2 cut(s) 41, 340
NlaIV GGNNCC 1 cut(s) 200
NmuCI GTSAC 1 cut(s) 233
PciSI GCTCTTC 1 cut(s) 3
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PfeI GAWTC 2 cut(s) 68, 124
PflFI GACNNNGTC 1 cut(s) 114
PkrI GCNGC 3 cut(s) 225, 245, 319
PleI GAGTC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 192
Psp124BI GAGCTC 2 cut(s) 15, 379
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 1 cut(s) 110
PsuI RGATCY 2 cut(s) 19, 198
PsyI GACNNNGTC 1 cut(s) 114
SacI GAGCTC 2 cut(s) 15, 379
SapI GCTCTTC 1 cut(s) 3
SatI GCNGC 3 cut(s) 224, 244, 318
Sau3AI GATC 3 cut(s) 19, 87, 198
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 1 cut(s) 193
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 2 cut(s) 15, 379
SfaNI GCATC 1 cut(s) 141
SinI GGWCC 1 cut(s) 110
SsiI CCGC 6 cut(s) 169, 223, 243, 315, 318, 359
SstI GAGCTC 2 cut(s) 15, 379
StyD4I CCNGG 1 cut(s) 300
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 2 cut(s) 195, 239
TaiI ACGT 1 cut(s) 118
TaqI TCGA 7 cut(s) 54, 90, 158, 179, 190, 215, 252
TauI GCSGC 3 cut(s) 226, 246, 320
TfiI GAWTC 2 cut(s) 68, 124
TseFI GTSAC 1 cut(s) 233
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 2 cut(s) 17, 353
Tth111I GACNNNGTC 1 cut(s) 114
VpaK11BI GGWCC 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.