Rw6G040170

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000672
Physical Location & Seq
Forward (+)
64030476 .. 64032680
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040170.1

Sequence Viewer

Length: 759 bp
ATGACCACCATATTTGAGAGACGTGAATTTACCACCGCCGTTTATCAAAGGTATGAAAATATAACCTACCGAAGCAAATCCTGCGTGGACGTACTTCTGATCAAATTCTATCGGTGGAAGGCATATTCAGACCATAACCCAGATATGCAAGATTTTGACGCTAGACCTCCAATTTTGTCATCTACAAAAGTTCTCAAGTTGCGGTTCTCAAGGGAGAGGGCAAGTGGAAATGCAAAGACAACGATGGAGAGACTTGCTAGGTACCAAAGGGTCATAGCTGGTCCCTGCAGGGAAGCAAACAAGTTACAGAACAAGATGGGTAGGTACCTTACCACAACTATTGGAATTCACGAAGATGAGCAACCGTTTATTATTGTAAAAATATTGGAAATGCTCCGAAAAGCCGTCCCCGATAAAATTATAGAAGTGCACATGCTGGAAATGACATTTACAAGTACGAATTGGTCCTTTCTGTGGGGCTTCAACGAGGACGGGGACGATATATACTACAGGGCTAAGCTGGTTCCTGCTCCGAAATCATTCATCGAGTCTCTGGAGGAAGTGAGACTACTTGATGATGATAATCTGGAATTGCTTGGATTGGAGTGTCCTATTTGTATGCAGGTGTTTGCAGAGAAAAAAGATACCAGCATTACTCGGTTGCCATGCTCACACTATTATCATCGACATTGCATTGTCCAGTGGCTCCAGATCAATCATGTCTGTCCCACTTGTCGACACCCTAATGCTCCGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ zf-RING_2

Protein Analysis

252

Amino Acids

29.63

Weight (kDa)

8.36

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 202 - 246 5.8e-13 Ring finger domain
zf-RING_11 PF17123 202 - 232 3e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 202 - 245 1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 203 - 245 9.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 206 - 246 1e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 613
Acc36I ACCTGC 1 cut(s) 613
Acc65I GGTACC 2 cut(s) 261, 324
AccB1I GGYRCC 2 cut(s) 261, 324
AccI GTMKAC 1 cut(s) 736
AciI CCGC 2 cut(s) 36, 202
AcsI RAATTY 3 cut(s) 26, 104, 345
AfaI GTAC 4 cut(s) 93, 263, 326, 457
AfiI CCNNNNNNNGG 1 cut(s) 474
AgsI TTSAA 1 cut(s) 484
AjiI CACGTC 1 cut(s) 23
AluBI AGCT 2 cut(s) 278, 520
AluI AGCT 2 cut(s) 278, 520
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 4 cut(s) 13, 244, 555, 559
Alw44I GTGCAC 1 cut(s) 428
ApaLI GTGCAC 1 cut(s) 428
ApoI RAATTY 3 cut(s) 26, 104, 345
Asp700I GAANNNNTTC 1 cut(s) 539
Asp718I GGTACC 2 cut(s) 261, 324
AspS9I GGNCC 2 cut(s) 281, 465
AvaII GGWCC 2 cut(s) 281, 465
BaeGI GKGCMC 1 cut(s) 432
BanI GGYRCC 2 cut(s) 261, 324
BarI GAAGNNNNNNTAC 2 cut(s) 552, 584
Bbv12I GWGCWC 1 cut(s) 432
BccI CCATC 2 cut(s) 238, 310
BceAI ACGGC 2 cut(s) 23, 389
BclI TGATCA 1 cut(s) 99
BcoDI GTCTC 4 cut(s) 13, 244, 555, 559
BfaI CTAG 2 cut(s) 162, 258
BfmI CTRYAG 2 cut(s) 286, 508
BfuAI ACCTGC 1 cut(s) 613
BlpI GCTNAGC 1 cut(s) 516
Bme18I GGWCC 2 cut(s) 281, 465
BmgBI CACGTC 1 cut(s) 23
BmgT120I GGNCC 2 cut(s) 281, 465
BmiI GGNNCC 5 cut(s) 263, 283, 326, 525, 707
BpmI CTGGAG 2 cut(s) 575, 692
Bpu1102I GCTNAGC 1 cut(s) 516
BpuEI CTTGAG 2 cut(s) 179, 193
BsaBI GATNNNNATC 1 cut(s) 582
Bsc4I CCNNNNNNNGG 1 cut(s) 474
Bse1I ACTGG 1 cut(s) 700
Bse3DI GCAATG 1 cut(s) 688
Bse8I GATNNNNATC 1 cut(s) 582
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 1 cut(s) 474
BseMI GCAATG 1 cut(s) 688
BseNI ACTGG 1 cut(s) 700
BseSI GKGCMC 1 cut(s) 432
BshNI GGYRCC 2 cut(s) 261, 324
BsiHKAI GWGCWC 1 cut(s) 432
BslFI GGGAC 4 cut(s) 267, 392, 509, 711
BslI CCNNNNNNNGG 1 cut(s) 474
BsmAI GTCTC 4 cut(s) 13, 244, 555, 559
BsmBI CGTCTC 1 cut(s) 13
BsmFI GGGAC 4 cut(s) 267, 392, 509, 711
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 2 cut(s) 99, 711
Bsp1720I GCTNAGC 1 cut(s) 516
BspACI CCGC 2 cut(s) 36, 202
BspLI GGNNCC 5 cut(s) 263, 283, 326, 525, 707
BspMAI CTGCAG 1 cut(s) 290
BspMI ACCTGC 1 cut(s) 613
BspT107I GGYRCC 2 cut(s) 261, 324
BsrDI GCAATG 1 cut(s) 688
BsrI ACTGG 1 cut(s) 700
BssMI GATC 2 cut(s) 99, 711
Bst4CI ACNGT 1 cut(s) 366
BstAPI GCANNNNNTGC 1 cut(s) 81
BstDEI CTNAG 1 cut(s) 516
BstKTI GATC 2 cut(s) 102, 714
BstMAI GTCTC 4 cut(s) 13, 244, 555, 559
BstMBI GATC 2 cut(s) 99, 711
BstMWI GCNNNNNNNGC 1 cut(s) 81
BstNSI RCATGY 1 cut(s) 436
BstSFI CTRYAG 2 cut(s) 286, 508
BstSLI GKGCMC 1 cut(s) 432
BtrI CACGTC 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 707
BveI ACCTGC 1 cut(s) 613
Cfr13I GGNCC 2 cut(s) 281, 465
CseI GACGC 1 cut(s) 167
Csp6I GTAC 4 cut(s) 92, 262, 325, 456
CspCI CAANNNNNGTGG 3 cut(s) 30, 322, 357
CviAII CATG 3 cut(s) 433, 666, 719
CviJI RGCY 6 cut(s) 278, 404, 480, 515, 520, 706
CviKI_1 RGCY 6 cut(s) 278, 404, 480, 515, 520, 706
CviQI GTAC 4 cut(s) 92, 262, 325, 456
DdeI CTNAG 1 cut(s) 516
DpnI GATC 2 cut(s) 101, 713
DpnII GATC 2 cut(s) 99, 711
Eco47I GGWCC 2 cut(s) 281, 465
EcoRI GAATTC 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 13
FaeI CATG 3 cut(s) 436, 669, 722
FaqI GGGAC 4 cut(s) 267, 392, 509, 711
FatI CATG 3 cut(s) 432, 665, 718
FbaI TGATCA 1 cut(s) 99
FblI GTMKAC 1 cut(s) 736
FspBI CTAG 2 cut(s) 162, 258
GsuI CTGGAG 2 cut(s) 575, 692
HgaI GACGC 1 cut(s) 167
Hin1II CATG 3 cut(s) 436, 669, 722
HincII GTYRAC 1 cut(s) 737
HindII GTYRAC 1 cut(s) 737
HinfI GANTC 1 cut(s) 548
Hpy166II GTNNAC 3 cut(s) 88, 430, 737
Hpy188I TCNGA 5 cut(s) 99, 130, 398, 534, 753
Hpy188III TCNNGA 4 cut(s) 350, 554, 587, 709
Hpy8I GTNNAC 3 cut(s) 88, 430, 737
HpyAV CCTTC 1 cut(s) 112
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4IV ACGT 2 cut(s) 22, 90
HpyCH4V TGCA 7 cut(s) 148, 233, 288, 430, 622, 632, 693
HpyF10VI GCNNNNNNNGC 1 cut(s) 81
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 2 cut(s) 22, 90
Hsp92II CATG 3 cut(s) 436, 669, 722
KpnI GGTACC 2 cut(s) 265, 328
Ksp22I TGATCA 1 cut(s) 99
Kzo9I GATC 2 cut(s) 99, 711
LmnI GCTCC 4 cut(s) 399, 535, 711, 754
MaeI CTAG 2 cut(s) 162, 258
MaeII ACGT 2 cut(s) 22, 90
MaeIII GTNAC 1 cut(s) 303
MalI GATC 2 cut(s) 101, 713
MboI GATC 2 cut(s) 99, 711
MboII GAAGA 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 7 cut(s) 26, 104, 171, 345, 417, 460, 590
MlyI GAGTC 1 cut(s) 557
MnlI CCTC 4 cut(s) 177, 210, 481, 550
MroXI GAANNNNTTC 1 cut(s) 539
MseI TTAA 1 cut(s) 757
MslI CAYNNNNRTG 2 cut(s) 354, 744
MwoI GCNNNNNNNGC 1 cut(s) 81
NdeII GATC 2 cut(s) 99, 711
NlaIII CATG 3 cut(s) 436, 669, 722
NlaIV GGNNCC 5 cut(s) 263, 283, 326, 525, 707
NspI RCATGY 1 cut(s) 436
PaqCI CACCTGC 1 cut(s) 613
PdmI GAANNNNTTC 1 cut(s) 539
PleI GAGTC 1 cut(s) 556
PpsI GAGTC 1 cut(s) 556
PspN4I GGNNCC 5 cut(s) 263, 283, 326, 525, 707
PspPI GGNCC 2 cut(s) 281, 465
PstI CTGCAG 1 cut(s) 290
RsaI GTAC 4 cut(s) 93, 263, 326, 457
RsaNI GTAC 4 cut(s) 92, 262, 325, 456
RseI CAYNNNNRTG 2 cut(s) 354, 744
SalI GTCGAC 1 cut(s) 735
SaqAI TTAA 1 cut(s) 757
Sau3AI GATC 2 cut(s) 99, 711
Sau96I GGNCC 2 cut(s) 281, 465
SbfI CCTGCAGG 1 cut(s) 290
SchI GAGTC 1 cut(s) 557
SdaI CCTGCAGG 1 cut(s) 290
SduI GDGCHC 1 cut(s) 432
SfcI CTRYAG 2 cut(s) 286, 508
SinI GGWCC 2 cut(s) 281, 465
SmiMI CAYNNNNRTG 2 cut(s) 354, 744
SmlI CTYRAG 2 cut(s) 194, 208
SmoI CTYRAG 2 cut(s) 194, 208
Sse8387I CCTGCAGG 1 cut(s) 290
Sse9I AATT 7 cut(s) 26, 104, 171, 345, 417, 460, 590
SsiI CCGC 2 cut(s) 36, 202
SspI AATATT 1 cut(s) 384
SspMI CTAG 2 cut(s) 162, 258
TaaI ACNGT 1 cut(s) 366
TaiI ACGT 2 cut(s) 25, 93
TaqI TCGA 3 cut(s) 546, 685, 736
TasI AATT 7 cut(s) 26, 104, 171, 345, 417, 460, 590
Tru1I TTAA 1 cut(s) 757
Tru9I TTAA 1 cut(s) 757
TscAI CASTG 1 cut(s) 707
TspDTI ATGAA 2 cut(s) 69, 532
TspRI CASTG 1 cut(s) 707
VneI GTGCAC 1 cut(s) 428
VpaK11BI GGWCC 2 cut(s) 281, 465
XapI RAATTY 3 cut(s) 26, 104, 345
XceI RCATGY 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 736
XmnI GAANNNNTTC 1 cut(s) 539
XspI CTAG 2 cut(s) 162, 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.