FvH4_3g15631

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000672
Physical Location & Seq
Reverse (-)
9813518 .. 9817124
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15631.t1

Sequence Viewer

Length: 462 bp
ATGGAATTCGAACTTCCCAATCCTCTTGCAAAACCTCGCCCTCAAACCCATATCTCAAATGACCTTACTGCTCAACCATCGGACATTCCCGAAGACCTCGACGACGACGTCCTCTGCTTCGCCCAACCCAAGTTAATCGTGGCTCCGATGGTGGAAAACTCCAAGGCATCTTCGAGAATCAAAGCACGACGAGGCGCAAAGGCGTGCACGCGTTTTAGTTTTTATTGGTTGAGGCCGAAGCGGAGGTCGTCTAGGAGTCTTCAACTGTGGATCCAGAAGTCATCGTTGAAGCGGCGGCAACTTTGTCACGACAGCGGCGGAATCGAAGTGGAAGAATGGATAGAGAGAGAAGGTTCAGATCTATGGTTTCCCATCCCGGTCTTCTTCTCCGCCGCCGACGACTGGACCGCCATGAAGGACGTGCCAACCACGGCGGACGACACTTGTGGAGCTCTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

17.41

Weight (kDa)

6.13

Isoelectric Point (pI)

73.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 107
AatII GACGTC 1 cut(s) 111
AccII CGCG 1 cut(s) 211
AciI CCGC 9 cut(s) 241, 292, 295, 315, 318, 390, 393, 408, 434
AclWI GGATC 2 cut(s) 265, 278
AcsI RAATTY 1 cut(s) 5
AcyI GRCGYC 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 402
AflIII ACRYGT 1 cut(s) 209
AgsI TTSAA 2 cut(s) 263, 289
AjiI CACGTC 1 cut(s) 421
AluBI AGCT 1 cut(s) 452
AluI AGCT 1 cut(s) 452
Alw21I GWGCWC 2 cut(s) 209, 454
Alw44I GTGCAC 1 cut(s) 205
AlwI GGATC 2 cut(s) 265, 278
AoxI GGCC 1 cut(s) 233
ApaLI GTGCAC 1 cut(s) 205
ApoI RAATTY 1 cut(s) 5
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AspLEI GCGC 1 cut(s) 197
AspS9I GGNCC 1 cut(s) 405
AsuC2I CCSGG 1 cut(s) 377
AsuII TTCGAA 1 cut(s) 9
AvaII GGWCC 1 cut(s) 405
BaeGI GKGCMC 1 cut(s) 209
BamHI GGATCC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 454
BbsI GAAGAC 3 cut(s) 99, 251, 373
Bbv12I GWGCWC 2 cut(s) 209, 454
BccI CCATC 3 cut(s) 85, 142, 380
BceAI ACGGC 1 cut(s) 447
BcnI CCSGG 1 cut(s) 377
BfaI CTAG 1 cut(s) 252
BglII AGATCT 1 cut(s) 358
BisI GCNGC 4 cut(s) 293, 296, 316, 393
BlsI GCNGC 4 cut(s) 294, 297, 317, 394
Bme1390I CCNGG 1 cut(s) 377
Bme18I GGWCC 1 cut(s) 405
BmgBI CACGTC 1 cut(s) 421
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 2 cut(s) 144, 272
BmrFI CCNGG 1 cut(s) 377
BmsI GCATC 1 cut(s) 176
BpiI GAAGAC 3 cut(s) 99, 251, 373
Bpu14I TTCGAA 1 cut(s) 9
BpuMI CCSGG 1 cut(s) 377
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 162, 429
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse1I ACTGG 1 cut(s) 407
BseDI CCNNGG 2 cut(s) 162, 429
BseGI GGATG 1 cut(s) 372
BseLI CCNNNNNNNGG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 407
BseSI GKGCMC 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 211
BshFI GGCC 1 cut(s) 235
BsiHKAI GWGCWC 2 cut(s) 209, 454
BsiSI CCGG 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 402
BsnI GGCC 1 cut(s) 235
Bsp119I TTCGAA 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 209, 454
Bsp143I GATC 2 cut(s) 270, 358
BspACI CCGC 9 cut(s) 241, 292, 295, 315, 318, 390, 393, 408, 434
BspANI GGCC 1 cut(s) 235
BspFNI CGCG 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 144, 272
BspPI GGATC 2 cut(s) 265, 278
BspT104I TTCGAA 1 cut(s) 9
BsrI ACTGG 1 cut(s) 407
BssECI CCNNGG 2 cut(s) 162, 429
BssMI GATC 2 cut(s) 270, 358
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 162
Bst4CI ACNGT 1 cut(s) 267
BstACI GRCGYC 1 cut(s) 108
BstBI TTCGAA 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 205, 209
BstDSI CCRYGG 1 cut(s) 429
BstF5I GGATG 1 cut(s) 372
BstFNI CGCG 1 cut(s) 211
BstHHI GCGC 1 cut(s) 197
BstKTI GATC 2 cut(s) 273, 361
BstMBI GATC 2 cut(s) 270, 358
BstSCI CCNGG 1 cut(s) 375
BstSLI GKGCMC 1 cut(s) 209
BstUI CGCG 1 cut(s) 211
BstV2I GAAGAC 3 cut(s) 99, 251, 373
BstX2I RGATCY 2 cut(s) 270, 358
BstYI RGATCY 2 cut(s) 270, 358
BsuRI GGCC 1 cut(s) 235
BtgI CCRYGG 1 cut(s) 429
BtrI CACGTC 1 cut(s) 421
BtsCI GGATG 1 cut(s) 372
Cac8I GCNNGC 2 cut(s) 205, 209
CfoI GCGC 1 cut(s) 197
Cfr13I GGNCC 1 cut(s) 405
CviAII CATG 1 cut(s) 412
CviJI RGCY 3 cut(s) 143, 235, 452
CviKI_1 RGCY 3 cut(s) 143, 235, 452
DpnI GATC 2 cut(s) 272, 360
DpnII GATC 2 cut(s) 270, 358
DrdI GACNNNNNNGTC 1 cut(s) 107
DseDI GACNNNNNNGTC 1 cut(s) 107
EciI GGCGGA 3 cut(s) 333, 379, 449
Ecl136II GAGCTC 1 cut(s) 452
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 405
Eco53kI GAGCTC 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 1 cut(s) 415
FaiI YATR 3 cut(s) 51, 364, 413
FatI CATG 1 cut(s) 411
Fnu4HI GCNGC 4 cut(s) 293, 296, 316, 393
FokI GGATG 1 cut(s) 359
FriOI GRGCYC 1 cut(s) 454
Fsp4HI GCNGC 4 cut(s) 293, 296, 316, 393
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 196
GluI GCNGC 4 cut(s) 293, 296, 316, 393
HaeIII GGCC 1 cut(s) 235
HapII CCGG 1 cut(s) 377
HhaI GCGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 1 cut(s) 415
Hin6I GCGC 1 cut(s) 195
HinP1I GCGC 1 cut(s) 195
HinfI GANTC 3 cut(s) 177, 256, 321
HpaII CCGG 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 207
Hpy188I TCNGA 3 cut(s) 82, 147, 358
Hpy188III TCNNGA 4 cut(s) 89, 174, 274, 308
Hpy8I GTNNAC 1 cut(s) 207
Hpy99I CGWCG 5 cut(s) 104, 107, 110, 192, 401
HpyAV CCTTC 2 cut(s) 344, 409
HpyCH4III ACNGT 1 cut(s) 267
HpyCH4IV ACGT 2 cut(s) 108, 420
HpyCH4V TGCA 2 cut(s) 29, 207
HpySE526I ACGT 2 cut(s) 108, 420
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 1 cut(s) 415
HspAI GCGC 1 cut(s) 195
Kzo9I GATC 2 cut(s) 270, 358
LmnI GCTCC 2 cut(s) 148, 449
LpnPI CCDG 3 cut(s) 287, 388, 390
LweI GCATC 1 cut(s) 176
MaeI CTAG 1 cut(s) 252
MaeII ACGT 2 cut(s) 108, 420
MaeIII GTNAC 1 cut(s) 305
MalI GATC 2 cut(s) 272, 360
MboI GATC 2 cut(s) 270, 358
MboII GAAGA 6 cut(s) 104, 162, 251, 344, 373, 376
MflI RGATCY 2 cut(s) 270, 358
MhlI GDGCHC 2 cut(s) 209, 454
MluCI AATT 1 cut(s) 5
MluI ACGCGT 1 cut(s) 209
MlyI GAGTC 1 cut(s) 265
MnlI CCTC 8 cut(s) 33, 45, 51, 107, 122, 185, 225, 237
MseI TTAA 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 315
MspI CCGG 1 cut(s) 377
MspR9I CCNGG 1 cut(s) 377
MvnI CGCG 1 cut(s) 211
NciI CCSGG 1 cut(s) 377
NdeII GATC 2 cut(s) 270, 358
NlaIII CATG 1 cut(s) 415
NlaIV GGNNCC 2 cut(s) 144, 272
NmuCI GTSAC 1 cut(s) 305
NspV TTCGAA 1 cut(s) 9
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PfeI GAWTC 2 cut(s) 177, 321
PflFI GACNNNGTC 1 cut(s) 107
PkrI GCNGC 4 cut(s) 294, 297, 317, 394
PleI GAGTC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 454
PspN4I GGNNCC 2 cut(s) 144, 272
PspPI GGNCC 1 cut(s) 405
PsuI RGATCY 2 cut(s) 270, 358
PsyI GACNNNGTC 1 cut(s) 107
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 1 cut(s) 134
SatI GCNGC 4 cut(s) 293, 296, 316, 393
Sau3AI GATC 2 cut(s) 270, 358
Sau96I GGNCC 1 cut(s) 405
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 2 cut(s) 209, 454
SetI ASST 8 cut(s) 37, 66, 99, 111, 248, 355, 423, 454
SfaNI GCATC 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 9
SinI GGWCC 1 cut(s) 405
Sse9I AATT 1 cut(s) 5
SsiI CCGC 9 cut(s) 241, 292, 295, 315, 318, 390, 393, 408, 434
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 454
StyD4I CCNGG 1 cut(s) 375
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 1 cut(s) 267
TaiI ACGT 2 cut(s) 111, 423
TaqI TCGA 4 cut(s) 9, 99, 173, 324
TasI AATT 1 cut(s) 5
TauI GCSGC 4 cut(s) 295, 298, 318, 395
TfiI GAWTC 2 cut(s) 177, 321
Tru1I TTAA 1 cut(s) 134
Tru9I TTAA 1 cut(s) 134
TseFI GTSAC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 1 cut(s) 428
Tth111I GACNNNGTC 1 cut(s) 107
VneI GTGCAC 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 405
XapI RAATTY 1 cut(s) 5
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XspI CTAG 1 cut(s) 252
ZraI GACGTC 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.